MD11G1000200.v1.1

Alpha-ketoglutarate-dependent dioxygenase alkB homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0005744
Physical Location & Seq
Reverse (-)
37245 .. 42991
5747 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1000200.v1.1.491

Sequence Viewer

Length: 804 bp
ATGGAAAAAAAAGTGACCTTAGATGATTTCAAAGTTGGATCTGTTCCGACTTTAATATACATCCCGGACTTCATTACGGATAATGAACAAACCATGCTTCTAAATAAGATTTATGAAGGCCCAGTATCAAAGTGGAAATCATTAAAGAATAGGAGGTTACAAAACTGGGGCGGTTTTGTCCATGAAAAGGGTCTTCTGCCACAGGATTTGCCATCATGGCTAACCAAGATTACATGCAAGATATATGAAGAATCAGGGCTGTTCCCATTGCCAATTAATCATGTCCTTATCAATGAATACCTTCCTAACCAAGGCATAATGGCACACCAGGACGGGCCGGCTTATTATCCAGTTGTAGCAATTCTGTCCCTCGAGTCACCTGTTGTTATGGACTTTACTCCCCATTCAAGACTGACTTTGTGCAAAAGCACTTGTGCAAATGCTGTTGAGGATACAAATTCTGATGCGGGGGTCATTAATATTAATACAGATAAGTCAATGAATGAACTTCATCCATTTCCTGTCATATTAATGCCTCGCAGTTTGCTGATATTCAAAGATAAAGCATACTCAGATTACTTGCATGCAATAAAAGACTGTGAGGTACAATGTTATGATGGGGCTGTAAACGAAGTACAAGCTCTACAGCGTGGTCAAGCTATAGATGATGATGCTTCACAATTTGATGGAGCTGTTGGTGTAATGAAAACTGGGGATTTTAAGAGAATTCATAGAACTACCCGTAGAATTTCATTGACATGTCGATTAGTTTCAAAAGTTCATAAAAATTTGCTCAGGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy_2

Protein Analysis

268

Amino Acids

30.2

Weight (kDa)

6.7

Isoelectric Point (pI)

36.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy_2 PF13532 68 - 255 5.1e-08 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 171, 467
AclWI GGATC 1 cut(s) 46
AcsI RAATTY 4 cut(s) 457, 726, 747, 787
AfaI GTAC 2 cut(s) 606, 636
AfiI CCNNNNNNNGG 2 cut(s) 187, 311
AflIII ACRYGT 1 cut(s) 758
AgsI TTSAA 4 cut(s) 31, 408, 556, 774
AjnI CCWGG 1 cut(s) 327
AluBI AGCT 3 cut(s) 641, 659, 692
AluI AGCT 3 cut(s) 641, 659, 692
AlwI GGATC 1 cut(s) 46
Ama87I CYCGRG 1 cut(s) 371
AoxI GGCC 2 cut(s) 118, 335
ApoI RAATTY 4 cut(s) 457, 726, 747, 787
AseI ATTAAT 4 cut(s) 276, 477, 483, 530
Asp700I GAANNNNTTC 1 cut(s) 300
AspS9I GGNCC 2 cut(s) 119, 335
AsuC2I CCSGG 1 cut(s) 65
AsuHPI GGTGA 1 cut(s) 369
AvaI CYCGRG 1 cut(s) 371
BbsI GAAGAC 1 cut(s) 185
BccI CCATC 3 cut(s) 220, 611, 680
BciT130I CCWGG 1 cut(s) 329
BciVI GTATCC 1 cut(s) 445
BcnI CCSGG 1 cut(s) 65
BfmI CTRYAG 2 cut(s) 644, 660
BfuI GTATCC 1 cut(s) 445
BglI GCCNNNNNGGC 1 cut(s) 217
Bme1390I CCNGG 2 cut(s) 65, 329
BmeT110I CYCGRG 1 cut(s) 371
BmgT120I GGNCC 2 cut(s) 119, 335
BmrFI CCNGG 2 cut(s) 65, 329
BmrI ACTGGG 3 cut(s) 116, 175, 720
BmsI GCATC 2 cut(s) 454, 661
BmuI ACTGGG 3 cut(s) 116, 175, 720
BpiI GAAGAC 1 cut(s) 185
Bpu10I CCTNAGC 1 cut(s) 794
BpuMI CCSGG 1 cut(s) 65
BsaJI CCNNGG 1 cut(s) 310
Bsc4I CCNNNNNNNGG 2 cut(s) 187, 311
Bse118I RCCGGY 1 cut(s) 337
Bse1I ACTGG 4 cut(s) 122, 170, 350, 715
Bse3DI GCAATG 1 cut(s) 266
BseBI CCWGG 1 cut(s) 329
BseDI CCNNGG 1 cut(s) 310
BseGI GGATG 2 cut(s) 60, 511
BseLI CCNNNNNNNGG 2 cut(s) 187, 311
BseMI GCAATG 1 cut(s) 266
BseMII CTCAG 1 cut(s) 585
BseNI ACTGG 4 cut(s) 122, 170, 350, 715
BshFI GGCC 2 cut(s) 120, 337
BsiHKCI CYCGRG 1 cut(s) 371
BsiSI CCGG 2 cut(s) 65, 338
BslFI GGGAC 1 cut(s) 352
BslI CCNNNNNNNGG 2 cut(s) 187, 311
BsmFI GGGAC 1 cut(s) 352
BsnI GGCC 2 cut(s) 120, 337
BsoBI CYCGRG 1 cut(s) 371
Bsp143I GATC 1 cut(s) 38
BspACI CCGC 2 cut(s) 171, 467
BspANI GGCC 2 cut(s) 120, 337
BspCNI CTCAG 1 cut(s) 584
BspPI GGATC 1 cut(s) 46
BsrDI GCAATG 1 cut(s) 266
BsrFI RCCGGY 1 cut(s) 337
BsrI ACTGG 4 cut(s) 122, 170, 350, 715
BssAI RCCGGY 1 cut(s) 337
BssECI CCNNGG 1 cut(s) 310
BssMI GATC 1 cut(s) 38
BssT1I CCWWGG 1 cut(s) 310
Bst2UI CCWGG 1 cut(s) 329
Bst4CI ACNGT 1 cut(s) 599
BstC8I GCNNGC 2 cut(s) 339, 585
BstDEI CTNAG 3 cut(s) 19, 571, 794
BstENI CCTNNNNNAGG 1 cut(s) 309
BstF5I GGATG 2 cut(s) 60, 511
BstKTI GATC 1 cut(s) 41
BstMBI GATC 1 cut(s) 38
BstMWI GCNNNNNNNGC 1 cut(s) 217
BstNI CCWGG 1 cut(s) 329
BstNSI RCATGY 3 cut(s) 237, 587, 762
BstSCI CCNGG 2 cut(s) 63, 327
BstSFI CTRYAG 2 cut(s) 644, 660
BstV2I GAAGAC 1 cut(s) 185
BstX2I RGATCY 1 cut(s) 38
BstYI RGATCY 1 cut(s) 38
BsuI GTATCC 1 cut(s) 445
BsuRI GGCC 2 cut(s) 120, 337
BtsCI GGATG 2 cut(s) 60, 511
Cac8I GCNNGC 2 cut(s) 339, 585
Cfr10I RCCGGY 1 cut(s) 337
Cfr13I GGNCC 2 cut(s) 119, 335
Csp6I GTAC 2 cut(s) 605, 635
CspCI CAANNNNNGTGG 2 cut(s) 189, 224
CviAII CATG 7 cut(s) 94, 182, 216, 234, 281, 584, 759
CviJI RGCY 9 cut(s) 120, 220, 259, 337, 341, 623, 641, 659, 692
CviKI_1 RGCY 9 cut(s) 120, 220, 259, 337, 341, 623, 641, 659, 692
CviQI GTAC 2 cut(s) 605, 635
DdeI CTNAG 3 cut(s) 19, 571, 794
DpnI GATC 1 cut(s) 40
DpnII GATC 1 cut(s) 38
Eco130I CCWWGG 1 cut(s) 310
Eco88I CYCGRG 1 cut(s) 371
EcoNI CCTNNNNNAGG 1 cut(s) 309
EcoRI GAATTC 1 cut(s) 726
EcoRII CCWGG 1 cut(s) 327
EcoT14I CCWWGG 1 cut(s) 310
ErhI CCWWGG 1 cut(s) 310
FaeI CATG 7 cut(s) 97, 185, 219, 237, 284, 587, 762
FalI AAGNNNNNCTT 2 cut(s) 400, 432
FaqI GGGAC 1 cut(s) 352
FatI CATG 7 cut(s) 93, 181, 215, 233, 280, 583, 758
FauI CCCGC 1 cut(s) 460
FokI GGATG 2 cut(s) 47, 498
HaeIII GGCC 2 cut(s) 120, 337
HapII CCGG 2 cut(s) 65, 338
Hin1II CATG 7 cut(s) 97, 185, 219, 237, 284, 587, 762
HinfI GANTC 2 cut(s) 251, 374
HpaII CCGG 2 cut(s) 65, 338
HphI GGTGA 1 cut(s) 369
Hpy166II GTNNAC 1 cut(s) 628
Hpy188I TCNGA 3 cut(s) 48, 463, 574
Hpy188III TCNNGA 1 cut(s) 408
Hpy8I GTNNAC 1 cut(s) 628
HpyAV CCTTC 2 cut(s) 110, 311
HpyCH4III ACNGT 1 cut(s) 599
HpyCH4V TGCA 5 cut(s) 237, 423, 437, 583, 587
HpyF10VI GCNNNNNNNGC 1 cut(s) 217
HpyF3I CTNAG 3 cut(s) 19, 571, 794
Hsp92II CATG 7 cut(s) 97, 185, 219, 237, 284, 587, 762
KroI GCCGGC 1 cut(s) 337
KroNI GCCGGC 1 cut(s) 339
Kzo9I GATC 1 cut(s) 38
LmnI GCTCC 1 cut(s) 689
LweI GCATC 2 cut(s) 454, 661
MaeIII GTNAC 3 cut(s) 13, 156, 375
MalI GATC 1 cut(s) 40
MboI GATC 1 cut(s) 38
MboII GAAGA 2 cut(s) 185, 260
MflI RGATCY 1 cut(s) 38
MluCI AATT 7 cut(s) 273, 360, 457, 680, 726, 747, 787
MlyI GAGTC 1 cut(s) 383
MmeI TCCRAC 2 cut(s) 16, 71
MnlI CCTC 5 cut(s) 147, 380, 442, 546, 595
MroNI GCCGGC 1 cut(s) 337
MroXI GAANNNNTTC 1 cut(s) 300
MseI TTAA 7 cut(s) 53, 143, 276, 477, 483, 530, 720
MslI CAYNNNNRTG 2 cut(s) 530, 757
MspI CCGG 2 cut(s) 65, 338
MspR9I CCNGG 2 cut(s) 65, 329
MvaI CCWGG 1 cut(s) 329
MwoI GCNNNNNNNGC 1 cut(s) 217
NaeI GCCGGC 1 cut(s) 339
NciI CCSGG 1 cut(s) 65
NdeII GATC 1 cut(s) 38
NgoMIV GCCGGC 1 cut(s) 337
NlaIII CATG 7 cut(s) 97, 185, 219, 237, 284, 587, 762
NmuCI GTSAC 2 cut(s) 13, 375
NspI RCATGY 3 cut(s) 237, 587, 762
PaeI GCATGC 1 cut(s) 587
PaeR7I CTCGAG 1 cut(s) 371
PciI ACATGT 1 cut(s) 758
PdiI GCCGGC 1 cut(s) 339
PdmI GAANNNNTTC 1 cut(s) 300
PfeI GAWTC 1 cut(s) 251
PfoI TCCNGGA 1 cut(s) 63
PleI GAGTC 1 cut(s) 382
PpsI GAGTC 1 cut(s) 382
PscI ACATGT 1 cut(s) 758
PshBI ATTAAT 4 cut(s) 276, 477, 483, 530
Psp6I CCWGG 1 cut(s) 327
PspGI CCWGG 1 cut(s) 327
PspPI GGNCC 2 cut(s) 119, 335
PspXI VCTCGAGB 1 cut(s) 371
PsuI RGATCY 1 cut(s) 38
RsaI GTAC 2 cut(s) 606, 636
RsaNI GTAC 2 cut(s) 605, 635
RseI CAYNNNNRTG 2 cut(s) 530, 757
SaqAI TTAA 7 cut(s) 53, 143, 276, 477, 483, 530, 720
Sau3AI GATC 1 cut(s) 38
Sau96I GGNCC 2 cut(s) 119, 335
SchI GAGTC 1 cut(s) 383
ScrFI CCNGG 2 cut(s) 65, 329
SetI ASST 9 cut(s) 20, 158, 303, 382, 606, 643, 661, 694, 800
SfaNI GCATC 2 cut(s) 454, 661
SfcI CTRYAG 2 cut(s) 644, 660
Sfr274I CTCGAG 1 cut(s) 371
SlaI CTCGAG 1 cut(s) 371
SmiMI CAYNNNNRTG 2 cut(s) 530, 757
SmlI CTYRAG 1 cut(s) 371
SmoI CTYRAG 1 cut(s) 371
SphI GCATGC 1 cut(s) 587
Sse9I AATT 7 cut(s) 273, 360, 457, 680, 726, 747, 787
SsiI CCGC 2 cut(s) 171, 467
SspI AATATT 1 cut(s) 481
StyD4I CCNGG 2 cut(s) 63, 327
StyI CCWWGG 1 cut(s) 310
TaaI ACNGT 1 cut(s) 599
TaqI TCGA 2 cut(s) 372, 763
TasI AATT 7 cut(s) 273, 360, 457, 680, 726, 747, 787
TatI WGTACW 1 cut(s) 634
TfiI GAWTC 1 cut(s) 251
Tru1I TTAA 7 cut(s) 53, 143, 276, 477, 483, 530, 720
Tru9I TTAA 7 cut(s) 53, 143, 276, 477, 483, 530, 720
TseFI GTSAC 2 cut(s) 13, 375
Tsp45I GTSAC 2 cut(s) 13, 375
TspGWI ACGGA 1 cut(s) 92
VspI ATTAAT 4 cut(s) 276, 477, 483, 530
XagI CCTNNNNNAGG 1 cut(s) 309
XapI RAATTY 4 cut(s) 457, 726, 747, 787
XceI RCATGY 3 cut(s) 237, 587, 762
XcmI CCANNNNNNNNNTGG 1 cut(s) 129
XhoI CTCGAG 1 cut(s) 371
XmnI GAANNNNTTC 1 cut(s) 300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.