Rh5CG589900

Alpha-ketoglutarate-dependent dioxygenase alkB homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0005744
Physical Location & Seq
Forward (+)
83513848 .. 83519220
5373 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG589900.1

Sequence Viewer

Length: 801 bp
ATGGAAGGAAGCCAGGCAGTAAATAATTTCAATGTTGGATCTCTGCCCACTTTAATATACATTCCTGACTTCATTACAGAAAATGAAGAAACTATACTTCAAAAAAATATTTATGATGCCCCTGTATCGAAGTGGAAATCTTTAAAGAATAGGAGGCTACAAAACTGGGGTGGTGTAGTTCATGAAAAGGGTCTGATATCACAGGATTTGCCCTCATGGTTAACAAAGATCACATGCAGGATATATGAAGAATCTGGGCTATTCCCATCGCCAATTAACCATGTCCTTATCAATGAATATCTTCCTAACCAAGGCATAATGGGACACCAGGATGGGCCAGCTTACTATCCAGTTGTAGCAATTCTGTCTCTTGGGTCACCTGTTGTTATGAATTTCACTCCCCATTCAAGATTGACATTGGGCGCAGGCACATGTACACATAATGCTATGTATTCAAATTCTGATGTGGGACTTTTCAAGAGTGATACAGATAAGTCGTTGGATGAACACCACCCATTTCCTGTCTTATTGATGCCACGCAGTTTGCTTATATTCAAAGATAAAGCATACTCAGATTACTTGCATGCTATAAACGACAGTGAGGTACACAGTTATGATAAGGTTGTAAACGAAGCACAAACTCGACCTTGGCAAGGCCTAGATCATCCATCTTCACAATTGGATGGAACTGATGAAACATTGAATACTGTGGACCTCAAGAGTATTCATAGAACTACCACTAGAGTTTCTTTAACATGTCGATTAGTTTTGAAGGTTCATAAAAATTTGTTCAGGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy_2

Protein Analysis

266

Amino Acids

30.02

Weight (kDa)

6.3

Isoelectric Point (pI)

42.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy_2 PF13532 52 - 254 7.5e-11 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 46
AcsI RAATTY 3 cut(s) 391, 457, 784
AfaI GTAC 2 cut(s) 436, 606
AfiI CCNNNNNNNGG 2 cut(s) 311, 653
AflIII ACRYGT 2 cut(s) 431, 755
AgsI TTSAA 8 cut(s) 31, 101, 408, 456, 478, 556, 703, 772
AjnI CCWGG 2 cut(s) 12, 327
AluBI AGCT 1 cut(s) 341
AluI AGCT 1 cut(s) 341
Alw26I GTCTC 1 cut(s) 372
AlwI GGATC 1 cut(s) 46
AoxI GGCC 2 cut(s) 335, 655
ApoI RAATTY 3 cut(s) 391, 457, 784
Asp700I GAANNNNTTC 1 cut(s) 300
AspLEI GCGC 1 cut(s) 425
AspS9I GGNCC 2 cut(s) 335, 712
AsuHPI GGTGA 1 cut(s) 369
AvaII GGWCC 1 cut(s) 712
BarI GAAGNNNNNNTAC 2 cut(s) 78, 110
BccI CCATC 4 cut(s) 274, 326, 676, 677
BciT130I CCWGG 2 cut(s) 14, 329
BcoDI GTCTC 1 cut(s) 372
BfaI CTAG 2 cut(s) 659, 741
Bme1390I CCNGG 2 cut(s) 14, 329
Bme18I GGWCC 1 cut(s) 712
BmgT120I GGNCC 2 cut(s) 335, 712
BmrFI CCNGG 2 cut(s) 14, 329
BmrI ACTGGG 1 cut(s) 175
BmsI GCATC 2 cut(s) 106, 522
BmuI ACTGGG 1 cut(s) 175
BpuEI CTTGAG 1 cut(s) 701
BsaJI CCNNGG 2 cut(s) 310, 647
Bsc4I CCNNNNNNNGG 2 cut(s) 311, 653
Bse1I ACTGG 2 cut(s) 170, 350
BseBI CCWGG 2 cut(s) 14, 329
BseDI CCNNGG 2 cut(s) 310, 647
BseGI GGATG 4 cut(s) 337, 508, 664, 688
BseLI CCNNNNNNNGG 2 cut(s) 311, 653
BseMII CTCAG 1 cut(s) 585
BseNI ACTGG 2 cut(s) 170, 350
BshFI GGCC 2 cut(s) 337, 657
BslFI GGGAC 2 cut(s) 336, 483
BslI CCNNNNNNNGG 2 cut(s) 311, 653
BsmAI GTCTC 1 cut(s) 372
BsmFI GGGAC 2 cut(s) 336, 483
BsnI GGCC 2 cut(s) 337, 657
Bsp1407I TGTACA 1 cut(s) 434
Bsp143I GATC 3 cut(s) 38, 228, 661
BspANI GGCC 2 cut(s) 337, 657
BspCNI CTCAG 1 cut(s) 584
BspHI TCATGA 1 cut(s) 181
BspPI GGATC 1 cut(s) 46
BsrGI TGTACA 1 cut(s) 434
BsrI ACTGG 2 cut(s) 170, 350
BssECI CCNNGG 2 cut(s) 310, 647
BssMI GATC 3 cut(s) 38, 228, 661
BssT1I CCWWGG 2 cut(s) 310, 647
Bst2UI CCWGG 2 cut(s) 14, 329
Bst4CI ACNGT 3 cut(s) 599, 611, 709
BstAUI TGTACA 1 cut(s) 434
BstC8I GCNNGC 3 cut(s) 339, 427, 585
BstDEI CTNAG 1 cut(s) 571
BstEII GGTNACC 1 cut(s) 375
BstENI CCTNNNNNAGG 2 cut(s) 309, 651
BstF5I GGATG 4 cut(s) 337, 508, 664, 688
BstHHI GCGC 1 cut(s) 425
BstKTI GATC 3 cut(s) 41, 231, 664
BstMAI GTCTC 1 cut(s) 372
BstMBI GATC 3 cut(s) 38, 228, 661
BstNI CCWGG 2 cut(s) 14, 329
BstNSI RCATGY 4 cut(s) 237, 435, 587, 759
BstPI GGTNACC 1 cut(s) 375
BstSCI CCNGG 2 cut(s) 12, 327
BstX2I RGATCY 1 cut(s) 38
BstYI RGATCY 1 cut(s) 38
BsuRI GGCC 2 cut(s) 337, 657
BtgZI GCGATG 1 cut(s) 252
BtsCI GGATG 4 cut(s) 337, 508, 664, 688
BtsIMutI CAGTG 1 cut(s) 604
Cac8I GCNNGC 3 cut(s) 339, 427, 585
CciI TCATGA 1 cut(s) 181
CfoI GCGC 1 cut(s) 425
Cfr13I GGNCC 2 cut(s) 335, 712
Csp6I GTAC 2 cut(s) 435, 605
CspCI CAANNNNNGTGG 2 cut(s) 525, 560
CviAII CATG 7 cut(s) 182, 216, 234, 281, 432, 584, 756
CviJI RGCY 6 cut(s) 12, 157, 259, 337, 341, 657
CviKI_1 RGCY 6 cut(s) 12, 157, 259, 337, 341, 657
CviQI GTAC 2 cut(s) 435, 605
DdeI CTNAG 1 cut(s) 571
DpnI GATC 3 cut(s) 40, 230, 663
DpnII GATC 3 cut(s) 38, 228, 661
DraI TTTAAA 1 cut(s) 144
Eco130I CCWWGG 2 cut(s) 310, 647
Eco147I AGGCCT 1 cut(s) 657
Eco32I GATATC 1 cut(s) 198
Eco47I GGWCC 1 cut(s) 712
Eco91I GGTNACC 1 cut(s) 375
EcoNI CCTNNNNNAGG 2 cut(s) 309, 651
EcoO65I GGTNACC 1 cut(s) 375
EcoRII CCWGG 2 cut(s) 12, 327
EcoRV GATATC 1 cut(s) 198
EcoT14I CCWWGG 2 cut(s) 310, 647
ErhI CCWWGG 2 cut(s) 310, 647
FaeI CATG 7 cut(s) 185, 219, 237, 284, 435, 587, 759
FaqI GGGAC 2 cut(s) 336, 483
FatI CATG 7 cut(s) 181, 215, 233, 280, 431, 583, 755
FokI GGATG 4 cut(s) 344, 515, 651, 695
FspBI CTAG 2 cut(s) 659, 741
GlaI GCGC 1 cut(s) 424
HaeIII GGCC 2 cut(s) 337, 657
HhaI GCGC 1 cut(s) 425
Hin1II CATG 7 cut(s) 185, 219, 237, 284, 435, 587, 759
Hin6I GCGC 1 cut(s) 423
HinP1I GCGC 1 cut(s) 423
HincII GTYRAC 1 cut(s) 222
HindII GTYRAC 1 cut(s) 222
HinfI GANTC 1 cut(s) 251
HpaI GTTAAC 1 cut(s) 222
HphI GGTGA 1 cut(s) 369
Hpy166II GTNNAC 5 cut(s) 222, 437, 607, 628, 712
Hpy188I TCNGA 3 cut(s) 195, 463, 574
Hpy188III TCNNGA 5 cut(s) 65, 182, 408, 478, 718
Hpy8I GTNNAC 5 cut(s) 222, 437, 607, 628, 712
HpyAV CCTTC 1 cut(s) 766
HpyCH4III ACNGT 3 cut(s) 599, 611, 709
HpyCH4V TGCA 2 cut(s) 237, 583
HpyF3I CTNAG 1 cut(s) 571
Hsp92II CATG 7 cut(s) 185, 219, 237, 284, 435, 587, 759
HspAI GCGC 1 cut(s) 423
KspAI GTTAAC 1 cut(s) 222
Kzo9I GATC 3 cut(s) 38, 228, 661
LweI GCATC 2 cut(s) 106, 522
MaeI CTAG 2 cut(s) 659, 741
MaeIII GTNAC 1 cut(s) 375
MalI GATC 3 cut(s) 40, 230, 663
MboI GATC 3 cut(s) 38, 228, 661
MboII GAAGA 4 cut(s) 98, 260, 293, 663
MfeI CAATTG 1 cut(s) 677
MflI RGATCY 1 cut(s) 38
MluCI AATT 7 cut(s) 25, 273, 360, 391, 457, 677, 784
MmeI TCCRAC 2 cut(s) 16, 480
MnlI CCTC 4 cut(s) 147, 223, 595, 725
MroXI GAANNNNTTC 1 cut(s) 300
MseI TTAA 5 cut(s) 53, 143, 221, 276, 752
MslI CAYNNNNRTG 2 cut(s) 330, 612
MspR9I CCNGG 2 cut(s) 14, 329
MunI CAATTG 1 cut(s) 677
MvaI CCWGG 2 cut(s) 14, 329
NdeII GATC 3 cut(s) 38, 228, 661
NlaIII CATG 7 cut(s) 185, 219, 237, 284, 435, 587, 759
NmuCI GTSAC 1 cut(s) 375
NspI RCATGY 4 cut(s) 237, 435, 587, 759
PaeI GCATGC 1 cut(s) 587
PagI TCATGA 1 cut(s) 181
PceI AGGCCT 1 cut(s) 657
PciI ACATGT 2 cut(s) 431, 755
PdmI GAANNNNTTC 1 cut(s) 300
PfeI GAWTC 1 cut(s) 251
PscI ACATGT 2 cut(s) 431, 755
Psp6I CCWGG 2 cut(s) 12, 327
PspEI GGTNACC 1 cut(s) 375
PspGI CCWGG 2 cut(s) 12, 327
PspPI GGNCC 2 cut(s) 335, 712
PsuI RGATCY 1 cut(s) 38
RsaI GTAC 2 cut(s) 436, 606
RsaNI GTAC 2 cut(s) 435, 605
RseI CAYNNNNRTG 2 cut(s) 330, 612
SaqAI TTAA 5 cut(s) 53, 143, 221, 276, 752
Sau3AI GATC 3 cut(s) 38, 228, 661
Sau96I GGNCC 2 cut(s) 335, 712
ScrFI CCNGG 2 cut(s) 14, 329
SetI ASST 8 cut(s) 343, 382, 606, 624, 649, 717, 777, 797
SfaNI GCATC 2 cut(s) 106, 522
SinI GGWCC 1 cut(s) 712
SmiMI CAYNNNNRTG 2 cut(s) 330, 612
SmlI CTYRAG 1 cut(s) 716
SmoI CTYRAG 1 cut(s) 716
SphI GCATGC 1 cut(s) 587
Sse9I AATT 7 cut(s) 25, 273, 360, 391, 457, 677, 784
SseBI AGGCCT 1 cut(s) 657
SspI AATATT 1 cut(s) 109
SspMI CTAG 2 cut(s) 659, 741
StuI AGGCCT 1 cut(s) 657
StyD4I CCNGG 2 cut(s) 12, 327
StyI CCWWGG 2 cut(s) 310, 647
TaaI ACNGT 3 cut(s) 599, 611, 709
TaqI TCGA 3 cut(s) 128, 643, 760
TasI AATT 7 cut(s) 25, 273, 360, 391, 457, 677, 784
TatI WGTACW 1 cut(s) 434
TfiI GAWTC 1 cut(s) 251
Tru1I TTAA 5 cut(s) 53, 143, 221, 276, 752
Tru9I TTAA 5 cut(s) 53, 143, 221, 276, 752
TscAI CASTG 1 cut(s) 604
TseFI GTSAC 1 cut(s) 375
Tsp45I GTSAC 1 cut(s) 375
TspRI CASTG 1 cut(s) 604
VpaK11BI GGWCC 1 cut(s) 712
XagI CCTNNNNNAGG 2 cut(s) 309, 651
XapI RAATTY 3 cut(s) 391, 457, 784
XceI RCATGY 4 cut(s) 237, 435, 587, 759
XmnI GAANNNNTTC 1 cut(s) 300
XspI CTAG 2 cut(s) 659, 741
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.