Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0002994

Physical Location & Seq

Genomic Coordinates Reverse (-)

11016809 .. 11021388

Gene Structure 4580 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT4G20430.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2571 bp

ATGAGGAGAGTTTTAATGGTGAATTTTGGGGTTTTACTTCTGTTCTGTTTTGGGGTTTTGTCGAATTCATTTGGACAAGATAATGGTGGTGATTCTGATATAAACTCTACTACGGCTGTTTACATTGTTACCCTGAGACAAGCTTCTTCTCTTCACCTTTTCCAACAAGAAGCAGAAGAAGTGAAAAGGGTCAGAGATCAATCCAAACATGGAGACACTTCCAAGTTTACTAGACCAAAACTCCAACCAAGGAACATTTCAAGGTCACGTTATTGGAGATCAAGAAGATCAGCAATTGCTCAAGCTCATGACTCTTTGCTAAGAAATGCATTGAAGGGAGAGAAGTATATAAAGCTCTATAGCTTCCATTATCTCATCAATGGTTTTGCTGTGTTTGTTAGCTCACAACAGGCTGAGACTCTTTCAAGAAGAAGGGAAGTAGCAAACATAGTGTTGGATTTTTCTGTTAGGACGGCAACAACTTATACACCTCAATTCATGGGTTTACCAAAAGGTGCATGGGTTAAAGAAGGTGGATATGAAACTGCTGGAGAAGGAATTGTTATCGGGTTTATCGACACTGGCATTGATCCAACTCACCCTAGTTTCAATGGCACTGACACTTCCCAGCGTCAGTATCCGATCCCTAATCATTTCTCAGGTGTTTGTGAAGTTACTCCGGATTTTCCATCAGGCTCCTGTAATAGGAAGCTTGTTGGAGCTCGCCATTTCGCGCAGTCAGCTATTACTAGAGGGATTTTCAATTCATCTGAAGACTATGCTTCACCTTTTGACGGCGATGGCCATGGCACGCACACAGCTTCCATCGCAGCGGGTAACCATGGTGTCTCAGCAGTAGTTTCTGGCCATAACTTTGGAAGTGCTAGTGGAATCGCTCCTCGTGCGCATATTTCTGTTTACAAGGCATTGTATAAGAGTTTTGGGGGATTTGCTGCAGATGTTGTTGCTGCTATAGACCAGGCAGCTCAAGATGGAGTTGACATATTAAGTCTATCCATCACACCTAATCGACGTCCTCCCGGTGTAGCCACGTTCTTTAACCCGTTAGATATGGCCATGCTTTCCGCTGTTAAGGCCGGTATATTCGTTGTACAAGCTGCAGGAAACACAGGTCCTTCTCCTAAGAGCATGTCCTCCTTCAGTCCATGGATTTTCACTGTTGGTGCTGCTTCTCACGATCGAGACTACTCTAACTCGATAGTCCTAGGCAACAATGTATCTATTCCAGGCGTAGGACTTGCACTTCGTACAGATGAAGGCAAGAAGTACACAATGATCTCTGCTCTTGATGCGTTGAAGAACAAAAGTTCAGTAGTAGACAAAGACATGTATGTAGGTGAATGCCAAGACTATGGCAGCTTCGATAAAGATGTTATCCGTGGGAATCTACTGATATGTAGCTACTCTATCCGTTTCGTCCTCGGGCTTTCCACTATTAAACAAGCTTTAGCTGTTGCCAAGAATCTATCTGCAAAGGGTGTTGTGTTCTACATGGACCCTTATGTTCTTGGTTTTCAGATTAATCCTACACCGATGGACATGCCCGGGATCATAATTCCATCCGCAGAAGATTCCAAGGTTTTACTCAAGTACTATAACTCTTCTCTTGTAAGAGATGGAACAACCAAGGAGATTGTTAGATTTGGAGCAGTTGCAGCCATTGCAGGTGGACAAAATGCTAACTTCAGTAACAGAGCTCCTAAGATTATGTACTACTCAGCAAGAGGACCTGATCCACAAGACAGCCTTTTTAACGACGCCGACATTTTGAAACCAAATCTTGTAGCTCCTGGAAATTCCATTTGGGGCGCTTGGAGTTCCGCTGCCACTGAATCCACTGAGTTTGAAGGAGAAAGTTTTGCGATGATGTCTGGTACGAGTATGGCTGCTCCTCATGTAGCTGGTGTGGCTGCATTGGTCAAGCAGAAATTCAGAAAGTTTAGTCCTTCAGCTATTGCATCTGCACTTTCAACAACCTCTGTTCTATTTGACAATAAAGGCGAGGCGATAATGGCTCAACGTGCTTATGCTAATCCTGATCAAACCATATCTCCTGCTACCCCATTTGATATGGGGAATGGCTTTGTGAATGCAACCGCAGCTTTGGATCCTGGCCTAATCTTTGATACTAGTTTTGAAGACTATATGTCATTTCTTTGTGGCATCAATGGATCAGCTCCAGTGGTATTCAACTACACCGGCACAAATTGCTTGCGGAACAATGCAACAATCAGCGGATCTGACCTTAATTTGCCATCCATCACTGTATCGAAGCTTAACAACACTCGAACGGTCCAAAGACTAATGACAAACATTGCTGGAAATGAGACCTATACTGTCAGTTTGATTACTCCTTTTGATGTTTTGATAAATGTATCTCCTACTCAATTCTCTATAGCCAGTGGAGAGACAAAGCTACTTAGTGTAATCCTCACAGCAAAGAGGAACAGCTCTATTTCTAGTTTTGGAGGGATCAAGCTGCTAGGAAACGCGGGACACATTGTTCGTATTCCGGTGTCTGTCACAGTGAAAATCGCCTCGAAACAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004252 GO:0005575 GO:0005618 GO:0005623 GO:0005911 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0009505 GO:0009506 GO:0016787 GO:0017171 GO:0019538 GO:0030054 GO:0030312 GO:0043170 GO:0044238 GO:0044464 GO:0055044 GO:0070011 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 Peptidase_S8

Protein Analysis

856

Amino Acids

91.81

Weight (kDa)

8.98

Isoelectric Point (pI)

35.43

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I9	PF05922	41 - 158	2.5e-11	Peptidase inhibitor I9
Peptidase_S8	PF00082	184 - 672	1.4e-45	Subtilase family
fn3_6	PF17766	755 - 848	5.8e-15	Fibronectin type-III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002994)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G30600 AT4G20430 AT4G20430 AT4G20430 AT5G44530 AT5G44530 AT5G44530
fragaria_vesca	FvH4_3g11550 FvH4_3g11550 FvH4_3g11550 FvH4_3g11550 FvH4_3g11550 FvH4_3g11550
malus_domestica	MD00G1096900.v1.1 MD10G1240800.v1.1
prunus_persica	Prupe.4G102800_v2.0.a1 Prupe.4G102800_v2.0.a1 Prupe.4G102800_v2.0.a1 Prupe.4G102800_v2.0.a1 Prupe.4G102800_v2.0.a1 Prupe.4G102800_v2.0.a1 Prupe.4G102800_v2.0.a1
pyrus_communis	pycom05g24650 pycom05g24660 pycom10g20150
rosa_chinensis	RchiOBHm_Chr5g0018491
rosa_laevigata	RLG00000032371
rosa_multiflora	Rmu_sc0002579.1_g000001 Rmu_sc0004464.1_g000018 Rmu_sc0038967.1_g000001
rosa_roxburghii	Rroxscaffold_1G00059240
rosa_rugosa	Rorug05G0044600 Rorug05G0044700
rosa_samantha	Rh5AG135800 Rh5BG134700 Rh5CG146800 Rh5DG135900
rosa_wichuraiana	Rw5G012030

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1676
AatII	GACGTC	1 cut(s)	1036
Acc16I	TGCGCA	1 cut(s)	906
Acc36I	ACCTGC	1 cut(s)	1676
AccI	GTMKAC	1 cut(s)	1338
AccII	CGCG	2 cut(s)	734, 2513
AccIII	TCCGGA	1 cut(s)	679
AciI	CCGC	8 cut(s)	833, 1086, 1584, 1842, 2118, 2236, 2256, 2513
AclWI	GGATC	9 cut(s)	584, 637, 1577, 1748, 2123, 2136, 2200, 2266, 2501
AcoI	YGGCCR	3 cut(s)	802, 865, 1074
AcsI	RAATTY	4 cut(s)	22, 64, 1816, 1949
AcuI	CTGAAG	4 cut(s)	792, 1144, 1690, 1953
AcyI	GRCGYC	2 cut(s)	1033, 1779
AfaI	GTAC	6 cut(s)	1113, 1270, 1289, 1613, 1733, 1897
AfiI	CCNNNNNNNGG	3 cut(s)	705, 794, 1253
AflIII	ACRYGT	1 cut(s)	1347
AhdI	GACNNNNNGTC	1 cut(s)	2167
AhlI	ACTAGT	1 cut(s)	2150
AjnI	CCWGG	4 cut(s)	978, 1246, 1810, 2131
AjuI	GAANNNNNNNTTGG	4 cut(s)	241, 273, 1807, 1839
AloI	GAACNNNNNNTCC	2 cut(s)	2508, 2540
Alw21I	GWGCWC	2 cut(s)	724, 1720
Alw26I	GTCTC	7 cut(s)	130, 207, 410, 853, 1197, 2342, 2423
AlwI	GGATC	9 cut(s)	584, 637, 1577, 1748, 2123, 2136, 2200, 2266, 2501
Ama87I	CYCGRG	2 cut(s)	1442, 1565
Aor13HI	TCCGGA	1 cut(s)	679
AoxI	GGCC	5 cut(s)	802, 865, 1074, 1095, 2134
ApoI	RAATTY	4 cut(s)	22, 64, 1816, 1949
ArsI	GACNNNNNNTTYG	4 cut(s)	1248, 1280, 2508, 2540
AseI	ATTAAT	1 cut(s)	1542
AspA2I	CCTAGG	1 cut(s)	1225
AspLEI	GCGC	3 cut(s)	736, 907, 1832
AspS9I	GGNCC	4 cut(s)	1133, 1516, 1748, 2314
AsuC2I	CCSGG	3 cut(s)	1041, 1566, 1567
AsuHPI	GGTGA	6 cut(s)	31, 101, 146, 590, 777, 1370
AvaI	CYCGRG	2 cut(s)	1442, 1565
AvaII	GGWCC	4 cut(s)	1133, 1516, 1748, 2314
AvrII	CCTAGG	1 cut(s)	1225
BalI	TGGCCA	3 cut(s)	804, 867, 1076
BamHI	GGATCC	1 cut(s)	2128
BanII	GRGCYC	2 cut(s)	724, 1720
BauI	CACGAG	1 cut(s)	900
BbsI	GAAGAC	2 cut(s)	780, 2166
Bbv12I	GWGCWC	2 cut(s)	724, 1720
BceAI	ACGGC	3 cut(s)	129, 489, 811
BcgI	CGANNNNNNTGC	2 cut(s)	1543, 1577
BciT130I	CCWGG	4 cut(s)	980, 1248, 1812, 2133
BciVI	GTATCC	1 cut(s)	648
BclI	TGATCA	1 cut(s)	2059
BcnI	CCSGG	3 cut(s)	1041, 1566, 1567
BcoDI	GTCTC	7 cut(s)	130, 207, 410, 853, 1197, 2342, 2423
BcuI	ACTAGT	1 cut(s)	2150
BfaI	CTAG	8 cut(s)	231, 603, 750, 885, 1226, 2151, 2481, 2504
BfmI	CTRYAG	5 cut(s)	358, 954, 972, 1119, 2415
BfoI	RGCGCY	1 cut(s)	1833
BfuAI	ACCTGC	1 cut(s)	1676
BfuI	GTATCC	1 cut(s)	648
BlnI	CCTAGG	1 cut(s)	1225
BmcAI	AGTACT	1 cut(s)	1613
Bme1390I	CCNGG	7 cut(s)	980, 1041, 1248, 1566, 1567, 1812, 2133
Bme18I	GGWCC	4 cut(s)	1133, 1516, 1748, 2314
BmeRI	GACNNNNNGTC	1 cut(s)	2167
BmeT110I	CYCGRG	2 cut(s)	1442, 1565
BmgT120I	GGNCC	4 cut(s)	1133, 1516, 1748, 2314
BmiI	GGNNCC	3 cut(s)	697, 1518, 2130
BmrFI	CCNGG	7 cut(s)	980, 1041, 1248, 1566, 1567, 1812, 2133
BmsI	GCATC	3 cut(s)	1300, 1988, 2193
BpiI	GAAGAC	2 cut(s)	780, 2166
BpmI	CTGGAG	2 cut(s)	570, 2184
BpuEI	CTTGAG	3 cut(s)	285, 972, 1592
BpuMI	CCSGG	3 cut(s)	1041, 1566, 1567
BsaHI	GRCGYC	2 cut(s)	1033, 1779
BsaI	GGTCTC	1 cut(s)	2342
BsaWI	WCCGGW	2 cut(s)	679, 2533
BsaXI	ACNNNNNCTCC	4 cut(s)	225, 255, 2056, 2086
Bsc4I	CCNNNNNNNGG	3 cut(s)	705, 794, 1253
Bse118I	RCCGGY	2 cut(s)	1097, 2219
Bse1I	ACTGG	3 cut(s)	586, 2201, 2421
Bse3DI	GCAATG	2 cut(s)	1680, 2334
BseAI	TCCGGA	1 cut(s)	679
BseBI	CCWGG	4 cut(s)	980, 1248, 1812, 2133
BseGI	GGATG	2 cut(s)	1580, 2276
BseLI	CCNNNNNNNGG	3 cut(s)	705, 794, 1253
BseMI	GCAATG	2 cut(s)	1680, 2334
BseMII	CTCAG	6 cut(s)	125, 405, 672, 864, 1752, 1851
BseNI	ACTGG	3 cut(s)	586, 2201, 2421
BseRI	GAGGAG	3 cut(s)	19, 888, 1902
BseYI	CCCAGC	1 cut(s)	627
BsgI	GTGCAG	1 cut(s)	1968
Bsh1236I	CGCG	2 cut(s)	734, 2513
Bsh1285I	CGRYCG	1 cut(s)	1201
BshFI	GGCC	5 cut(s)	804, 867, 1076, 1097, 2136
BsiEI	CGRYCG	1 cut(s)	1201
BsiHKAI	GWGCWC	2 cut(s)	724, 1720
BsiHKCI	CYCGRG	2 cut(s)	1442, 1565
BsiSI	CCGG	6 cut(s)	680, 1041, 1098, 1566, 2220, 2534
BslFI	GGGAC	1 cut(s)	2529
BslI	CCNNNNNNNGG	3 cut(s)	705, 794, 1253
BsmAI	GTCTC	7 cut(s)	130, 207, 410, 853, 1197, 2342, 2423
BsmFI	GGGAC	1 cut(s)	2529
BsmI	GAATGC	2 cut(s)	1367, 2116
BsnI	GGCC	5 cut(s)	804, 867, 1076, 1097, 2136
Bso31I	GGTCTC	1 cut(s)	2342
BsoBI	CYCGRG	2 cut(s)	1442, 1565
Bsp1286I	GDGCHC	2 cut(s)	724, 1720
Bsp13I	TCCGGA	1 cut(s)	679
Bsp1407I	TGTACA	1 cut(s)	1111
Bsp19I	CCATGG	3 cut(s)	805, 841, 1166
BspACI	CCGC	8 cut(s)	833, 1086, 1584, 1842, 2118, 2236, 2256, 2513
BspANI	GGCC	5 cut(s)	804, 867, 1076, 1097, 2136
BspCNI	CTCAG	6 cut(s)	126, 406, 671, 863, 1751, 1852
BspEI	TCCGGA	1 cut(s)	679
BspFNI	CGCG	2 cut(s)	734, 2513
BspHI	TCATGA	1 cut(s)	307
BspLI	GGNNCC	3 cut(s)	697, 1518, 2130
BspMAI	CTGCAG	2 cut(s)	958, 1123
BspMI	ACCTGC	1 cut(s)	1676
BspPI	GGATC	9 cut(s)	584, 637, 1577, 1748, 2123, 2136, 2200, 2266, 2501
BspTNI	GGTCTC	1 cut(s)	2342
BsrDI	GCAATG	2 cut(s)	1680, 2334
BsrFI	RCCGGY	2 cut(s)	1097, 2219
BsrGI	TGTACA	1 cut(s)	1111
BsrI	ACTGG	3 cut(s)	586, 2201, 2421
BssAI	RCCGGY	2 cut(s)	1097, 2219
BssNI	GRCGYC	2 cut(s)	1033, 1779
BssSI	CACGAG	1 cut(s)	900
BssT1I	CCWWGG	7 cut(s)	248, 805, 841, 1166, 1225, 1596, 1647
Bst2BI	CACGAG	1 cut(s)	900
Bst2UI	CCWGG	4 cut(s)	980, 1248, 1812, 2133
Bst4CI	ACNGT	5 cut(s)	1180, 2287, 2314, 2359, 2548
Bst6I	CTCTTC	2 cut(s)	156, 1627
BstACI	GRCGYC	2 cut(s)	1033, 1779
BstAPI	GCANNNNNTGC	2 cut(s)	1682, 2229
BstAUI	TGTACA	1 cut(s)	1111
BstC8I	GCNNGC	3 cut(s)	724, 812, 2234
BstDSI	CCRYGG	4 cut(s)	805, 841, 1166, 1399
BstEII	GGTNACC	1 cut(s)	836
BstENI	CCTNNNNNAGG	1 cut(s)	703
BstF5I	GGATG	2 cut(s)	1580, 2276
BstFNI	CGCG	2 cut(s)	734, 2513
BstH2I	RGCGCY	1 cut(s)	1833
BstHHI	GCGC	3 cut(s)	736, 907, 1832
BstMAI	GTCTC	7 cut(s)	130, 207, 410, 853, 1197, 2342, 2423
BstMCI	CGRYCG	1 cut(s)	1201
BstNI	CCWGG	4 cut(s)	980, 1248, 1812, 2133
BstNSI	RCATGY	3 cut(s)	1153, 1351, 1564
BstPI	GGTNACC	1 cut(s)	836
BstSCI	CCNGG	7 cut(s)	978, 1039, 1246, 1564, 1565, 1810, 2131
BstSFI	CTRYAG	5 cut(s)	358, 954, 972, 1119, 2415
BstUI	CGCG	2 cut(s)	734, 2513
BstV2I	GAAGAC	2 cut(s)	780, 2166
BstX2I	RGATCY	2 cut(s)	2128, 2258
BstXI	CCANNNNNNTGG	2 cut(s)	875, 1373
BstYI	RGATCY	2 cut(s)	2128, 2258
BsuI	GTATCC	1 cut(s)	648
BsuRI	GGCC	5 cut(s)	804, 867, 1076, 1097, 2136
BtgI	CCRYGG	4 cut(s)	805, 841, 1166, 1399
BtgZI	GCGATG	3 cut(s)	811, 813, 1898
BtsCI	GGATG	2 cut(s)	1580, 2276
BtsIMutI	CAGTG	9 cut(s)	579, 615, 1176, 1848, 1857, 2208, 2283, 2428, 2553
BveI	ACCTGC	1 cut(s)	1676
Cac8I	GCNNGC	3 cut(s)	724, 812, 2234
CciI	TCATGA	1 cut(s)	307
CfoI	GCGC	3 cut(s)	736, 907, 1832
Cfr10I	RCCGGY	2 cut(s)	1097, 2219
Cfr13I	GGNCC	4 cut(s)	1133, 1516, 1748, 2314
Cfr9I	CCCGGG	1 cut(s)	1565
CseI	GACGC	2 cut(s)	620, 1787
Csp6I	GTAC	6 cut(s)	1112, 1269, 1288, 1612, 1732, 1896
CspCI	CAANNNNNGTGG	2 cut(s)	1846, 1881
CviQI	GTAC	6 cut(s)	1112, 1269, 1288, 1612, 1732, 1896
DriI	GACNNNNNGTC	1 cut(s)	2167
EaeI	YGGCCR	3 cut(s)	802, 865, 1074
Eam1104I	CTCTTC	2 cut(s)	156, 1627
Eam1105I	GACNNNNNGTC	1 cut(s)	2167
EarI	CTCTTC	2 cut(s)	156, 1627
Ecl136II	GAGCTC	2 cut(s)	722, 1718
Eco130I	CCWWGG	7 cut(s)	248, 805, 841, 1166, 1225, 1596, 1647
Eco24I	GRGCYC	2 cut(s)	724, 1720
Eco31I	GGTCTC	1 cut(s)	2342
Eco47I	GGWCC	4 cut(s)	1133, 1516, 1748, 2314
Eco53kI	GAGCTC	2 cut(s)	722, 1718
Eco57I	CTGAAG	4 cut(s)	792, 1144, 1690, 1953
Eco88I	CYCGRG	2 cut(s)	1442, 1565
Eco91I	GGTNACC	1 cut(s)	836
EcoICRI	GAGCTC	2 cut(s)	722, 1718
EcoNI	CCTNNNNNAGG	1 cut(s)	703
EcoO109I	RGGNCCY	2 cut(s)	1133, 1748
EcoO65I	GGTNACC	1 cut(s)	836
EcoRI	GAATTC	1 cut(s)	64
EcoRII	CCWGG	4 cut(s)	978, 1246, 1810, 2131
EcoT14I	CCWWGG	7 cut(s)	248, 805, 841, 1166, 1225, 1596, 1647
EcoT22I	ATGCAT	1 cut(s)	331
EcoT38I	GRGCYC	2 cut(s)	724, 1720
ErhI	CCWWGG	7 cut(s)	248, 805, 841, 1166, 1225, 1596, 1647
FalI	AAGNNNNNCTT	2 cut(s)	1752, 1784
FaqI	GGGAC	1 cut(s)	2529
FauI	CCCGC	2 cut(s)	826, 2506
FbaI	TGATCA	1 cut(s)	2059
FblI	GTMKAC	1 cut(s)	1338
FokI	GGATG	2 cut(s)	1567, 2263
FriOI	GRGCYC	2 cut(s)	724, 1720
FspAI	RTGCGCAY	1 cut(s)	906
FspBI	CTAG	8 cut(s)	231, 603, 750, 885, 1226, 2151, 2481, 2504
FspI	TGCGCA	1 cut(s)	906
GlaI	GCGC	3 cut(s)	735, 906, 1831
GsaI	CCCAGC	1 cut(s)	631
GsuI	CTGGAG	2 cut(s)	570, 2184
HaeII	RGCGCY	1 cut(s)	1833
HaeIII	GGCC	5 cut(s)	804, 867, 1076, 1097, 2136
HapII	CCGG	6 cut(s)	680, 1041, 1098, 1566, 2220, 2534
HgaI	GACGC	2 cut(s)	620, 1787
HhaI	GCGC	3 cut(s)	736, 907, 1832
Hin1I	GRCGYC	2 cut(s)	1033, 1779
Hin6I	GCGC	3 cut(s)	734, 905, 1830
HinP1I	GCGC	3 cut(s)	734, 905, 1830
HincII	GTYRAC	1 cut(s)	1000
HindII	GTYRAC	1 cut(s)	1000
HindIII	AAGCTT	4 cut(s)	141, 710, 1464, 2294
HinfI	GANTC	8 cut(s)	92, 311, 418, 891, 1405, 1483, 1592, 1853
HpaII	CCGG	6 cut(s)	680, 1041, 1098, 1566, 2220, 2534
HphI	GGTGA	6 cut(s)	31, 101, 146, 590, 777, 1370
Hpy166II	GTNNAC	8 cut(s)	121, 228, 506, 919, 1000, 1290, 1339, 1691
Hpy188I	TCNGA	7 cut(s)	97, 194, 642, 772, 1539, 1955, 2263
Hpy188III	TCNNGA	9 cut(s)	282, 308, 426, 680, 989, 1196, 1202, 1307, 2057
Hpy8I	GTNNAC	8 cut(s)	121, 228, 506, 919, 1000, 1290, 1339, 1691
Hpy99I	CGWCG	2 cut(s)	1035, 1781
HpyAV	CCTTC	9 cut(s)	328, 426, 524, 548, 1146, 1168, 1271, 1862, 1977
HpyCH4III	ACNGT	5 cut(s)	1180, 2287, 2314, 2359, 2548
HpyCH4IV	ACGT	4 cut(s)	268, 1033, 1052, 2041
HpySE526I	ACGT	4 cut(s)	268, 1033, 1052, 2041
Hsp92I	GRCGYC	2 cut(s)	1033, 1779
HspAI	GCGC	3 cut(s)	734, 905, 1830
Kpn2I	TCCGGA	1 cut(s)	679
Ksp22I	TGATCA	1 cut(s)	2059
LmnI	GCTCC	8 cut(s)	701, 719, 901, 1667, 1723, 1813, 1915, 2203
LweI	GCATC	3 cut(s)	1300, 1988, 2193
MaeI	CTAG	8 cut(s)	231, 603, 750, 885, 1226, 2151, 2481, 2504
MaeII	ACGT	4 cut(s)	268, 1033, 1052, 2041
MaeIII	GTNAC	6 cut(s)	127, 264, 673, 836, 1709, 2542
MfeI	CAATTG	1 cut(s)	294
MflI	RGATCY	2 cut(s)	2128, 2258
MhlI	GDGCHC	2 cut(s)	724, 1720
MlsI	TGGCCA	3 cut(s)	804, 867, 1076
MluNI	TGGCCA	3 cut(s)	804, 867, 1076
MlyI	GAGTC	2 cut(s)	305, 412
MmeI	TCCRAC	5 cut(s)	187, 268, 435, 617, 697
Mox20I	TGGCCA	3 cut(s)	804, 867, 1076
Mph1103I	ATGCAT	1 cut(s)	331
MroI	TCCGGA	1 cut(s)	679
MscI	TGGCCA	3 cut(s)	804, 867, 1076
Msp20I	TGGCCA	3 cut(s)	804, 867, 1076
MspA1I	CMGCKG	4 cut(s)	833, 1088, 1844, 2256
MspI	CCGG	6 cut(s)	680, 1041, 1098, 1566, 2220, 2534
MspR9I	CCNGG	7 cut(s)	980, 1041, 1248, 1566, 1567, 1812, 2133
MunI	CAATTG	1 cut(s)	294
Mva1269I	GAATGC	2 cut(s)	1367, 2116
MvaI	CCWGG	4 cut(s)	980, 1248, 1812, 2133
MvnI	CGCG	2 cut(s)	734, 2513
NciI	CCSGG	3 cut(s)	1041, 1566, 1567
NcoI	CCATGG	3 cut(s)	805, 841, 1166
NlaIV	GGNNCC	3 cut(s)	697, 1518, 2130
NmuCI	GTSAC	2 cut(s)	264, 2542
NsbI	TGCGCA	1 cut(s)	906
NsiI	ATGCAT	1 cut(s)	331
NspI	RCATGY	3 cut(s)	1153, 1351, 1564
PagI	TCATGA	1 cut(s)	307
PaqCI	CACCTGC	1 cut(s)	1676
PciI	ACATGT	1 cut(s)	1347
PcsI	WCGNNNNNNNCGW	1 cut(s)	573
PctI	GAATGC	2 cut(s)	1367, 2116
PfeI	GAWTC	6 cut(s)	92, 891, 1405, 1483, 1592, 1853
PfoI	TCCNGGA	1 cut(s)	1810
Ple19I	CGATCG	1 cut(s)	1201
PleI	GAGTC	2 cut(s)	305, 412
PpsI	GAGTC	2 cut(s)	305, 412
PpuMI	RGGWCCY	2 cut(s)	1133, 1748
PscI	ACATGT	1 cut(s)	1347
PshBI	ATTAAT	1 cut(s)	1542
Psp124BI	GAGCTC	2 cut(s)	724, 1720
Psp5II	RGGWCCY	2 cut(s)	1133, 1748
Psp6I	CCWGG	4 cut(s)	978, 1246, 1810, 2131
PspEI	GGTNACC	1 cut(s)	836
PspFI	CCCAGC	1 cut(s)	627
PspGI	CCWGG	4 cut(s)	978, 1246, 1810, 2131
PspN4I	GGNNCC	3 cut(s)	697, 1518, 2130
PspPI	GGNCC	4 cut(s)	1133, 1516, 1748, 2314
PspPPI	RGGWCCY	2 cut(s)	1133, 1748
PstI	CTGCAG	2 cut(s)	958, 1123
PsuI	RGATCY	2 cut(s)	2128, 2258
PvuI	CGATCG	1 cut(s)	1201
RsaI	GTAC	6 cut(s)	1113, 1270, 1289, 1613, 1733, 1897
RsaNI	GTAC	6 cut(s)	1112, 1269, 1288, 1612, 1732, 1896
SacI	GAGCTC	2 cut(s)	724, 1720
Sau96I	GGNCC	4 cut(s)	1133, 1516, 1748, 2314
ScaI	AGTACT	1 cut(s)	1613
SchI	GAGTC	2 cut(s)	305, 412
ScrFI	CCNGG	7 cut(s)	980, 1041, 1248, 1566, 1567, 1812, 2133
SduI	GDGCHC	2 cut(s)	724, 1720
SfaNI	GCATC	3 cut(s)	1300, 1988, 2193
SfcI	CTRYAG	5 cut(s)	358, 954, 972, 1119, 2415
SinI	GGWCC	4 cut(s)	1133, 1516, 1748, 2314
SmaI	CCCGGG	1 cut(s)	1567
SmlI	CTYRAG	3 cut(s)	300, 987, 1607
SmoI	CTYRAG	3 cut(s)	300, 987, 1607
SpeI	ACTAGT	1 cut(s)	2150
SsiI	CCGC	8 cut(s)	833, 1086, 1584, 1842, 2118, 2236, 2256, 2513
SspMI	CTAG	8 cut(s)	231, 603, 750, 885, 1226, 2151, 2481, 2504
SstI	GAGCTC	2 cut(s)	724, 1720
StyD4I	CCNGG	7 cut(s)	978, 1039, 1246, 1564, 1565, 1810, 2131
StyI	CCWWGG	7 cut(s)	248, 805, 841, 1166, 1225, 1596, 1647
TaaI	ACNGT	5 cut(s)	1180, 2287, 2314, 2359, 2548
TaiI	ACGT	4 cut(s)	271, 1036, 1055, 2044
TaqI	TCGA	9 cut(s)	62, 576, 1030, 1201, 1217, 1383, 2291, 2308, 2561
TatI	WGTACW	4 cut(s)	1111, 1287, 1611, 1731
TfiI	GAWTC	6 cut(s)	92, 891, 1405, 1483, 1592, 1853
TscAI	CASTG	9 cut(s)	586, 622, 1183, 1855, 1864, 2208, 2290, 2428, 2553
TseFI	GTSAC	2 cut(s)	264, 2542
Tsp45I	GTSAC	2 cut(s)	264, 2542
TspDTI	ATGAA	5 cut(s)	57, 487, 555, 756, 1290
TspGWI	ACGGA	2 cut(s)	1388, 1421
TspMI	CCCGGG	1 cut(s)	1565
TspRI	CASTG	9 cut(s)	586, 622, 1183, 1855, 1864, 2208, 2290, 2428, 2553
VpaK11BI	GGWCC	4 cut(s)	1133, 1516, 1748, 2314
VspI	ATTAAT	1 cut(s)	1542
XagI	CCTNNNNNAGG	1 cut(s)	703
XapI	RAATTY	4 cut(s)	22, 64, 1816, 1949
XceI	RCATGY	3 cut(s)	1153, 1351, 1564
XcmI	CCANNNNNNNNNTGG	1 cut(s)	516
XmaI	CCCGGG	1 cut(s)	1565
XmaJI	CCTAGG	1 cut(s)	1225
XmiI	GTMKAC	1 cut(s)	1338
XspI	CTAG	8 cut(s)	231, 603, 750, 885, 1226, 2151, 2481, 2504
ZraI	GACGTC	1 cut(s)	1034
ZrmI	AGTACT	1 cut(s)	1613
Zsp2I	ATGCAT	1 cut(s)	331

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.