Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0002994

Physical Location & Seq

Genomic Coordinates Forward (+)

20350835 .. 20356067

Gene Structure 5233 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1096900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2184 bp

ATGGGAAGCTTCCATGGCGTGCTTTTGATGGGGGTGCTTTTTCTGGGGATGCTTATGACTTCTTTTTGTCAAGACGAAGATTCTGATAACTTCGCGGCTGTGTATATCGTTACTCTCAGAGAAGCTCCGGCCGTTCACTACGATGCAGAGTTGAGAAGGAGCAGCAAAAATGGTGTTAGACCTGGCAGTGCTTCTGAGAGGTTGAACATTCACAAACCAAGGTTTAGAAATATTTCGAGAAGGGATGGTAGGTATAGCTCTTATATCGCTCGAACTCATGATTCATTATTGAGGAGGGTGTTGAGAGGGGAGAAATATCTGAAGCTATACAGCTACCACTACTTGATAAACGGATTTGCTGTGTTTGTTACCCAAGATCAGGTTGATAAGCTTTCAAGGAGGAGAGAAGTAGCAAATGTGGTTTTGGACTTTTCTGTTAGAACTGCAACCACCCATACTCCTCAGTTCTTGGGTTTACCACAAGGAGCATGGGTTCAAGGAGGGGGATATGAATCAGCTGGAGAAGGGGTCGTTATTGGGTTTATCGATACTGGTATTGATCCAACTCACATTAGTTTTGATGACCATGCATCTGAACGTCCATATCCGGTTCCTCCCCATTTCTGTGGCATCTGCGAGGTTACTCCGGATTTCCCATCTGGGTCCTGCAATAGAAAGCTTATTGCAGCCCGCCATTTTGCATCATCCGCTATAACCAGAGGTGTTTTCAATTCATCTCAGGACTACGCTTCTCCATTTGACGGTGATGGTCATGGCACGCACACCGCTTCCATTGCTGCAGGAAACCATGGAATCCCAGTGGTTGTAGCTGGGCATAGTTTCGGAAATGCAAGTGGGATGGCTCCTCATGCACACATCGCTGTTTACAAGGCATTGTACAAAGGTTTTGGAGGCTTTGCGGCTGATGTTGTTGCTGCCATAGATCAGGCTGCTCAGGACGGGGTTGACATAATAAGCTTATCAATCACTCCCAACCGGCGTCCTCCCGGAGTTGCAACATTTTTCAATCCGATAGACATGGCATTACTATCAGCTGTAAAGGCTGGTATATTTGTTGTCCAAGCAGCAGGAAATACTGGACCATCACCAAAGAGCATGTCTTCCTTTAGTCCATGGATATTCACTGTTGGCTCTGCCTCTCATGACAGAAACTACAGCAATTCTATAATGCTTGGCAACAATGTCACCATTACTGGAGTTGGACTTGCATCGGGAACAGATTCAATGTACACAATGATCTCTGCCACACATGCTTTGAACAATGGCACACCAGTGTCAGATGATATGTATGTGGGTGAATGCCAAGACCCGAGCAACTTTAATCAGGATTTGATCCGGGGAAACCTCTTGATCTGTAGCTACTCAATTCGATTTGTGCTAGGAATCTCTACCATCAATAAATCCTTAGAAACAGCCAAAAACCTAAGTGCAGTTGGTGTTGTGTTCACCATGGATGCTTTTGTAATCGGTTTTCAGCTTAACCCAACTCCAATGAAAATACCTGGCATCATAATTCCATCCCCAGAAGATTCCAAGGTTCTAATGAAATACTACAATCTTTCCTTGGAGAGAGATATTGTGACAAAGAGAATTACCAAATTTGGGGCACTTGCAACCATTTGTGGAGGTTTGAAAGCAAATTACAGTGGTTCTGCCCCAAAGATCATGTATTACTCTGCTAGAGGTCCAGATCCGGAAGACAATTTTCTTGATGATGCAGACATAATGAAACCCAACTTGGTAGCTCCTGGAAATTCTATATGGGCTGCTTGGAGTTCTGTTGGGCAAGACTCGATTGAATTTCAAGGCGAGAACTTCGCAATTATGTCTGGGACAAGCATGGCTGCTCCTCATATTACTGGGCTTGCTGCACTGGTTAGGCAGAAGTTCCCCAATTTCAGTCCTTCAGCGATTGCATCTGCACTTTCCACAACTGCTTCTCTATATGACAAGAATGGTGGGCCCATAATGGCTCAGCGTGCATATGCATTTCCAGATCAAAACCAGTCTCCAGCAACGCCCTTTGATATGGGCAGTGGTTTTGTGAATGCCACAGCAGCGCTGAATCCAGGATTGATTTTTGATGCCAGTAGTTCGTATTACTCATGTTCATGTACTTTCTCAAACTGGATTAAAGGTTGGATACCATCAGATTCGCAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004252 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0016787 GO:0017171 GO:0019538 GO:0043170 GO:0044238 GO:0070011 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 Peptidase_S8

Protein Analysis

728

Amino Acids

78.05

Weight (kDa)

6.55

Isoelectric Point (pI)

40.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I9	PF05922	34 - 148	4.4e-13	Peptidase inhibitor I9
Peptidase_S8	PF00082	174 - 665	3.9e-45	Subtilase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002994)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G30600 AT4G20430 AT4G20430 AT4G20430 AT5G44530 AT5G44530 AT5G44530
fragaria_vesca	FvH4_3g11550 FvH4_3g11550 FvH4_3g11550 FvH4_3g11550 FvH4_3g11550 FvH4_3g11550
malus_domestica	MD00G1096900.v1.1 MD10G1240800.v1.1
prunus_persica	Prupe.4G102800_v2.0.a1 Prupe.4G102800_v2.0.a1 Prupe.4G102800_v2.0.a1 Prupe.4G102800_v2.0.a1 Prupe.4G102800_v2.0.a1 Prupe.4G102800_v2.0.a1 Prupe.4G102800_v2.0.a1
pyrus_communis	pycom05g24650 pycom05g24660 pycom10g20150
rosa_chinensis	RchiOBHm_Chr5g0018491
rosa_laevigata	RLG00000032371
rosa_multiflora	Rmu_sc0002579.1_g000001 Rmu_sc0004464.1_g000018 Rmu_sc0038967.1_g000001
rosa_roxburghii	Rroxscaffold_1G00059240
rosa_rugosa	Rorug05G0044600 Rorug05G0044700
rosa_samantha	Rh5AG135800 Rh5BG134700 Rh5CG146800 Rh5DG135900
rosa_wichuraiana	Rw5G012030

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	1 cut(s)	95
AccIII	TCCGGA	2 cut(s)	646, 1714
AciI	CCGC	5 cut(s)	95, 691, 708, 786, 920
AclWI	GGATC	3 cut(s)	554, 1348, 1706
AcoI	YGGCCR	1 cut(s)	129
AcsI	RAATTY	3 cut(s)	1619, 1774, 1820
AcuI	CTGAAG	2 cut(s)	341, 1911
AcyI	GRCGYC	1 cut(s)	1000
AfaI	GTAC	3 cut(s)	899, 1250, 2137
AfeI	AGCGCT	1 cut(s)	2082
AfiI	CCNNNNNNNGG	3 cut(s)	379, 761, 1623
AjnI	CCWGG	4 cut(s)	181, 1522, 1768, 2089
AjuI	GAANNNNNNNTTGG	2 cut(s)	1742, 1774
AleI	CACNNNNGTG	1 cut(s)	1292
AloI	GAACNNNNNNTCC	2 cut(s)	477, 509
Alw26I	GTCTC	1 cut(s)	2034
AlwI	GGATC	3 cut(s)	554, 1348, 1706
Ama87I	CYCGRG	1 cut(s)	1330
Aor13HI	TCCGGA	2 cut(s)	646, 1714
Aor51HI	AGCGCT	1 cut(s)	2082
AoxI	GGCC	2 cut(s)	129, 1982
ApaI	GGGCCC	1 cut(s)	1986
ApoI	RAATTY	3 cut(s)	1619, 1774, 1820
ArsI	GACNNNNNNTTYG	2 cut(s)	1103, 1135
Asp700I	GAANNNNTTC	2 cut(s)	232, 1240
AspLEI	GCGC	1 cut(s)	2083
AspS9I	GGNCC	5 cut(s)	663, 1100, 1706, 1982, 1983
AsuC2I	CCSGG	2 cut(s)	1008, 1358
AsuHPI	GGTGA	5 cut(s)	776, 1098, 1198, 1328, 1459
AvaI	CYCGRG	1 cut(s)	1330
AvaII	GGWCC	3 cut(s)	663, 1100, 1706
BaeGI	GKGCMC	2 cut(s)	1630, 1986
BanII	GRGCYC	1 cut(s)	1986
BbsI	GAAGAC	2 cut(s)	1113, 1725
BccI	CCATC	9 cut(s)	22, 239, 664, 761, 853, 1111, 1421, 1546, 2176
BceAI	ACGGC	1 cut(s)	116
BciT130I	CCWGG	4 cut(s)	183, 1524, 1770, 2091
BciVI	GTATCC	1 cut(s)	2157
BcnI	CCSGG	2 cut(s)	1008, 1358
BcoDI	GTCTC	1 cut(s)	2034
BfaI	CTAG	2 cut(s)	1400, 1701
BfmI	CTRYAG	3 cut(s)	798, 1174, 1375
BfoI	RGCGCY	1 cut(s)	2084
BfuI	GTATCC	1 cut(s)	2157
BlpI	GCTNAGC	1 cut(s)	1995
Bme1390I	CCNGG	6 cut(s)	183, 1008, 1358, 1524, 1770, 2091
Bme18I	GGWCC	3 cut(s)	663, 1100, 1706
BmeT110I	CYCGRG	1 cut(s)	1330
BmgT120I	GGNCC	5 cut(s)	663, 1100, 1706, 1982, 1983
BmiI	GGNNCC	4 cut(s)	612, 664, 864, 1984
BmrFI	CCNGG	6 cut(s)	183, 1008, 1358, 1524, 1770, 2091
BmrI	ACTGGG	2 cut(s)	812, 1890
BmuI	ACTGGG	2 cut(s)	812, 1890
BpiI	GAAGAC	2 cut(s)	1113, 1725
BpmI	CTGGAG	3 cut(s)	540, 1236, 2016
Bpu10I	CCTNAGC	1 cut(s)	954
Bpu1102I	GCTNAGC	1 cut(s)	1995
BpuMI	CCSGG	2 cut(s)	1008, 1358
Bsa29I	ATCGAT	1 cut(s)	546
BsaBI	GATNNNNATC	1 cut(s)	511
BsaHI	GRCGYC	1 cut(s)	1000
BsaJI	CCNNGG	8 cut(s)	13, 218, 808, 1133, 1357, 1470, 1554, 1584
BsaWI	WCCGGW	3 cut(s)	607, 646, 1714
BsaXI	ACNNNNNCTCC	6 cut(s)	442, 472, 477, 507, 513, 543
Bsc4I	CCNNNNNNNGG	3 cut(s)	379, 761, 1623
Bse118I	RCCGGY	1 cut(s)	996
Bse3DI	GCAATG	1 cut(s)	792
Bse8I	GATNNNNATC	1 cut(s)	511
BseAI	TCCGGA	2 cut(s)	646, 1714
BseBI	CCWGG	4 cut(s)	183, 1524, 1770, 2091
BseCI	ATCGAT	1 cut(s)	546
BseDI	CCNNGG	8 cut(s)	13, 218, 808, 1133, 1357, 1470, 1554, 1584
BseGI	GGATG	6 cut(s)	54, 250, 704, 864, 1480, 1538
BseJI	GATNNNNATC	1 cut(s)	511
BseLI	CCNNNNNNNGG	3 cut(s)	379, 761, 1623
BseMI	GCAATG	1 cut(s)	792
BseMII	CTCAG	6 cut(s)	130, 186, 476, 752, 968, 2009
BseRI	GAGGAG	5 cut(s)	307, 415, 450, 855, 1860
BseSI	GKGCMC	2 cut(s)	1630, 1986
BseX3I	CGGCCG	1 cut(s)	129
BseYI	CCCAGC	1 cut(s)	830
BsgI	GTGCAG	3 cut(s)	1470, 1875, 1926
Bsh1236I	CGCG	1 cut(s)	95
Bsh1285I	CGRYCG	1 cut(s)	132
BshFI	GGCC	2 cut(s)	131, 1984
BshVI	ATCGAT	1 cut(s)	546
BsiEI	CGRYCG	1 cut(s)	132
BsiHKCI	CYCGRG	1 cut(s)	1330
BsiSI	CCGG	7 cut(s)	128, 608, 647, 997, 1008, 1357, 1715
BslFI	GGGAC	1 cut(s)	1867
BslI	CCNNNNNNNGG	3 cut(s)	379, 761, 1623
BsmAI	GTCTC	1 cut(s)	2034
BsmFI	GGGAC	1 cut(s)	1867
BsmI	GAATGC	2 cut(s)	1325, 2074
BsnI	GGCC	2 cut(s)	131, 1984
BsoBI	CYCGRG	1 cut(s)	1330
Bsp120I	GGGCCC	1 cut(s)	1982
Bsp1286I	GDGCHC	2 cut(s)	1630, 1986
Bsp13I	TCCGGA	2 cut(s)	646, 1714
Bsp1407I	TGTACA	2 cut(s)	897, 1248
Bsp143I	GATC	9 cut(s)	376, 559, 943, 1257, 1353, 1371, 1683, 1711, 2017
Bsp1720I	GCTNAGC	1 cut(s)	1995
Bsp19I	CCATGG	4 cut(s)	13, 808, 1133, 1470
BspACI	CCGC	5 cut(s)	95, 691, 708, 786, 920
BspANI	GGCC	2 cut(s)	131, 1984
BspCNI	CTCAG	6 cut(s)	129, 187, 475, 751, 967, 2008
BspDI	ATCGAT	1 cut(s)	546
BspEI	TCCGGA	2 cut(s)	646, 1714
BspFNI	CGCG	1 cut(s)	95
BspHI	TCATGA	2 cut(s)	277, 1162
BspLI	GGNNCC	4 cut(s)	612, 664, 864, 1984
BspMAI	CTGCAG	1 cut(s)	802
BspPI	GGATC	3 cut(s)	554, 1348, 1706
BsrDI	GCAATG	1 cut(s)	792
BsrFI	RCCGGY	1 cut(s)	996
BsrGI	TGTACA	2 cut(s)	897, 1248
BssAI	RCCGGY	1 cut(s)	996
BssECI	CCNNGG	8 cut(s)	13, 218, 808, 1133, 1357, 1470, 1554, 1584
BssMI	GATC	9 cut(s)	376, 559, 943, 1257, 1353, 1371, 1683, 1711, 2017
BssNI	GRCGYC	1 cut(s)	1000
BssT1I	CCWWGG	7 cut(s)	13, 218, 808, 1133, 1470, 1554, 1584
Bst2UI	CCWGG	4 cut(s)	183, 1524, 1770, 2091
Bst4CI	ACNGT	3 cut(s)	764, 1147, 1667
BstACI	GRCGYC	1 cut(s)	1000
BstAUI	TGTACA	2 cut(s)	897, 1248
BstC8I	GCNNGC	5 cut(s)	20, 691, 779, 1887, 2001
BstDEI	CTNAG	8 cut(s)	116, 195, 462, 738, 954, 1426, 1445, 1995
BstDSI	CCRYGG	4 cut(s)	13, 808, 1133, 1470
BstF5I	GGATG	6 cut(s)	54, 250, 704, 864, 1480, 1538
BstFNI	CGCG	1 cut(s)	95
BstH2I	RGCGCY	1 cut(s)	2084
BstHHI	GCGC	1 cut(s)	2083
BstKTI	GATC	9 cut(s)	379, 562, 946, 1260, 1356, 1374, 1686, 1714, 2020
BstMAI	GTCTC	1 cut(s)	2034
BstMBI	GATC	9 cut(s)	376, 559, 943, 1257, 1353, 1371, 1683, 1711, 2017
BstMCI	CGRYCG	1 cut(s)	132
BstMWI	GCNNNNNNNGC	9 cut(s)	15, 707, 794, 869, 878, 1061, 1271, 2000, 2078
BstNI	CCWGG	4 cut(s)	183, 1524, 1770, 2091
BstNSI	RCATGY	2 cut(s)	1120, 1274
BstSCI	CCNGG	6 cut(s)	181, 1006, 1356, 1522, 1768, 2089
BstSFI	CTRYAG	3 cut(s)	798, 1174, 1375
BstSLI	GKGCMC	2 cut(s)	1630, 1986
BstUI	CGCG	1 cut(s)	95
BstV2I	GAAGAC	2 cut(s)	1113, 1725
BstX2I	RGATCY	1 cut(s)	1711
BstXI	CCANNNNNNTGG	1 cut(s)	626
BstYI	RGATCY	1 cut(s)	1711
BstZI	CGGCCG	1 cut(s)	129
Bsu15I	ATCGAT	1 cut(s)	546
BsuI	GTATCC	1 cut(s)	2157
BsuRI	GGCC	2 cut(s)	131, 1984
BsuTUI	ATCGAT	1 cut(s)	546
BtgI	CCRYGG	4 cut(s)	13, 808, 1133, 1470
BtgZI	GCGATG	1 cut(s)	862
BtsCI	GGATG	6 cut(s)	54, 250, 704, 864, 1480, 1538
BtsI	GCAGTG	2 cut(s)	193, 2062
BtsIMutI	CAGTG	7 cut(s)	193, 825, 1143, 1299, 1672, 1892, 2062
Cac8I	GCNNGC	5 cut(s)	20, 691, 779, 1887, 2001
CciI	TCATGA	2 cut(s)	277, 1162
CfoI	GCGC	1 cut(s)	2083
Cfr10I	RCCGGY	1 cut(s)	996
Cfr13I	GGNCC	5 cut(s)	663, 1100, 1706, 1982, 1983
ClaI	ATCGAT	1 cut(s)	546
CseI	GACGC	1 cut(s)	989
Csp6I	GTAC	3 cut(s)	898, 1249, 2136
CspCI	CAANNNNNGTGG	2 cut(s)	1960, 1995
CviQI	GTAC	3 cut(s)	898, 1249, 2136
DdeI	CTNAG	8 cut(s)	116, 195, 462, 738, 954, 1426, 1445, 1995
DpnI	GATC	9 cut(s)	378, 561, 945, 1259, 1355, 1373, 1685, 1713, 2019
DpnII	GATC	9 cut(s)	376, 559, 943, 1257, 1353, 1371, 1683, 1711, 2017
EaeI	YGGCCR	1 cut(s)	129
EagI	CGGCCG	1 cut(s)	129
EclXI	CGGCCG	1 cut(s)	129
Eco130I	CCWWGG	7 cut(s)	13, 218, 808, 1133, 1470, 1554, 1584
Eco24I	GRGCYC	1 cut(s)	1986
Eco47I	GGWCC	3 cut(s)	663, 1100, 1706
Eco47III	AGCGCT	1 cut(s)	2082
Eco52I	CGGCCG	1 cut(s)	129
Eco57I	CTGAAG	2 cut(s)	341, 1911
Eco88I	CYCGRG	1 cut(s)	1330
EcoO109I	RGGNCCY	1 cut(s)	663
EcoRII	CCWGG	4 cut(s)	181, 1522, 1768, 2089
EcoT14I	CCWWGG	7 cut(s)	13, 218, 808, 1133, 1470, 1554, 1584
EcoT22I	ATGCAT	2 cut(s)	592, 2011
EcoT38I	GRGCYC	1 cut(s)	1986
ErhI	CCWWGG	7 cut(s)	13, 218, 808, 1133, 1470, 1554, 1584
FaqI	GGGAC	1 cut(s)	1867
FauI	CCCGC	1 cut(s)	698
FauNDI	CATATG	1 cut(s)	2005
FokI	GGATG	6 cut(s)	61, 257, 691, 871, 1487, 1525
FriOI	GRGCYC	1 cut(s)	1986
FspBI	CTAG	2 cut(s)	1400, 1701
GlaI	GCGC	1 cut(s)	2082
GsaI	CCCAGC	1 cut(s)	834
GsuI	CTGGAG	3 cut(s)	540, 1236, 2016
HaeII	RGCGCY	1 cut(s)	2084
HaeIII	GGCC	2 cut(s)	131, 1984
HapII	CCGG	7 cut(s)	128, 608, 647, 997, 1008, 1357, 1715
HgaI	GACGC	1 cut(s)	989
HhaI	GCGC	1 cut(s)	2083
Hin1I	GRCGYC	1 cut(s)	1000
Hin6I	GCGC	1 cut(s)	2081
HinP1I	GCGC	1 cut(s)	2081
HincII	GTYRAC	1 cut(s)	967
HindII	GTYRAC	1 cut(s)	967
HindIII	AAGCTT	4 cut(s)	7, 389, 677, 976
HpaII	CCGG	7 cut(s)	128, 608, 647, 997, 1008, 1357, 1715
HphI	GGTGA	5 cut(s)	776, 1098, 1198, 1328, 1459
Hpy166II	GTNNAC	6 cut(s)	136, 476, 886, 967, 1251, 1467
Hpy188I	TCNGA	9 cut(s)	85, 119, 196, 321, 595, 845, 1032, 1300, 2173
Hpy8I	GTNNAC	6 cut(s)	136, 476, 886, 967, 1251, 1467
HpyAV	CCTTC	4 cut(s)	150, 234, 518, 1935
HpyCH4III	ACNGT	3 cut(s)	764, 1147, 1667
HpyCH4IV	ACGT	1 cut(s)	598
HpyF10VI	GCNNNNNNNGC	9 cut(s)	15, 707, 794, 869, 878, 1061, 1271, 2000, 2078
HpyF3I	CTNAG	8 cut(s)	116, 195, 462, 738, 954, 1426, 1445, 1995
HpySE526I	ACGT	1 cut(s)	598
Hsp92I	GRCGYC	1 cut(s)	1000
HspAI	GCGC	1 cut(s)	2081
Kpn2I	TCCGGA	2 cut(s)	646, 1714
Kzo9I	GATC	9 cut(s)	376, 559, 943, 1257, 1353, 1371, 1683, 1711, 2017
LmnI	GCTCC	6 cut(s)	130, 159, 485, 868, 1771, 1873
MaeI	CTAG	2 cut(s)	1400, 1701
MaeII	ACGT	1 cut(s)	598
MaeIII	GTNAC	5 cut(s)	109, 367, 640, 1204, 1600
MalI	GATC	9 cut(s)	378, 561, 945, 1259, 1355, 1373, 1685, 1713, 2019
MboI	GATC	9 cut(s)	376, 559, 943, 1257, 1353, 1371, 1683, 1711, 2017
MboII	GAAGA	4 cut(s)	89, 1113, 1559, 1730
MflI	RGATCY	1 cut(s)	1711
MhlI	GDGCHC	2 cut(s)	1630, 1986
MlyI	GAGTC	1 cut(s)	1805
MmeI	TCCRAC	3 cut(s)	587, 1201, 2141
Mph1103I	ATGCAT	2 cut(s)	592, 2011
MroI	TCCGGA	2 cut(s)	646, 1714
MroXI	GAANNNNTTC	2 cut(s)	232, 1240
MseI	TTAA	3 cut(s)	1341, 1500, 2154
MslI	CAYNNNNRTG	4 cut(s)	141, 624, 1292, 2131
MspA1I	CMGCKG	2 cut(s)	518, 1055
MspI	CCGG	7 cut(s)	128, 608, 647, 997, 1008, 1357, 1715
MspR9I	CCNGG	6 cut(s)	183, 1008, 1358, 1524, 1770, 2091
Mva1269I	GAATGC	2 cut(s)	1325, 2074
MvaI	CCWGG	4 cut(s)	183, 1524, 1770, 2091
MvnI	CGCG	1 cut(s)	95
MwoI	GCNNNNNNNGC	9 cut(s)	15, 707, 794, 869, 878, 1061, 1271, 2000, 2078
NciI	CCSGG	2 cut(s)	1008, 1358
NcoI	CCATGG	4 cut(s)	13, 808, 1133, 1470
NdeI	CATATG	1 cut(s)	2005
NdeII	GATC	9 cut(s)	376, 559, 943, 1257, 1353, 1371, 1683, 1711, 2017
NlaIV	GGNNCC	4 cut(s)	612, 664, 864, 1984
NmuCI	GTSAC	2 cut(s)	1204, 1600
NsiI	ATGCAT	2 cut(s)	592, 2011
NspI	RCATGY	2 cut(s)	1120, 1274
OliI	CACNNNNGTG	1 cut(s)	1292
PagI	TCATGA	2 cut(s)	277, 1162
PctI	GAATGC	2 cut(s)	1325, 2074
PdmI	GAANNNNTTC	2 cut(s)	232, 1240
PfeI	GAWTC	9 cut(s)	80, 281, 512, 813, 1241, 1404, 1550, 2086, 2174
PfoI	TCCNGGA	3 cut(s)	1006, 1768, 2089
PleI	GAGTC	1 cut(s)	1805
PpsI	GAGTC	1 cut(s)	1805
PpuMI	RGGWCCY	1 cut(s)	663
Psp5II	RGGWCCY	1 cut(s)	663
Psp6I	CCWGG	4 cut(s)	181, 1522, 1768, 2089
PspFI	CCCAGC	1 cut(s)	830
PspGI	CCWGG	4 cut(s)	181, 1522, 1768, 2089
PspN4I	GGNNCC	4 cut(s)	612, 664, 864, 1984
PspOMI	GGGCCC	1 cut(s)	1982
PspPI	GGNCC	5 cut(s)	663, 1100, 1706, 1982, 1983
PspPPI	RGGWCCY	1 cut(s)	663
PstI	CTGCAG	1 cut(s)	802
PsuI	RGATCY	1 cut(s)	1711
PvuII	CAGCTG	2 cut(s)	518, 1055
RsaI	GTAC	3 cut(s)	899, 1250, 2137
RsaNI	GTAC	3 cut(s)	898, 1249, 2136
RseI	CAYNNNNRTG	4 cut(s)	141, 624, 1292, 2131
SaqAI	TTAA	3 cut(s)	1341, 1500, 2154
Sau3AI	GATC	9 cut(s)	376, 559, 943, 1257, 1353, 1371, 1683, 1711, 2017
Sau96I	GGNCC	5 cut(s)	663, 1100, 1706, 1982, 1983
SchI	GAGTC	1 cut(s)	1805
ScrFI	CCNGG	6 cut(s)	183, 1008, 1358, 1524, 1770, 2091
SduI	GDGCHC	2 cut(s)	1630, 1986
SfcI	CTRYAG	3 cut(s)	798, 1174, 1375
SinI	GGWCC	3 cut(s)	663, 1100, 1706
SmiMI	CAYNNNNRTG	4 cut(s)	141, 624, 1292, 2131
SsiI	CCGC	5 cut(s)	95, 691, 708, 786, 920
SspI	AATATT	1 cut(s)	232
SspMI	CTAG	2 cut(s)	1400, 1701
StyD4I	CCNGG	6 cut(s)	181, 1006, 1356, 1522, 1768, 2089
StyI	CCWWGG	7 cut(s)	13, 218, 808, 1133, 1470, 1554, 1584
TaaI	ACNGT	3 cut(s)	764, 1147, 1667
TaiI	ACGT	1 cut(s)	601
TaqI	TCGA	5 cut(s)	236, 271, 546, 1390, 1814
TatI	WGTACW	3 cut(s)	897, 1248, 2135
TauI	GCSGC	2 cut(s)	98, 923
TfiI	GAWTC	9 cut(s)	80, 281, 512, 813, 1241, 1404, 1550, 2086, 2174
Tru1I	TTAA	3 cut(s)	1341, 1500, 2154
Tru9I	TTAA	3 cut(s)	1341, 1500, 2154
TscAI	CASTG	7 cut(s)	193, 825, 1150, 1299, 1672, 1899, 2062
TseFI	GTSAC	2 cut(s)	1204, 1600
Tsp45I	GTSAC	2 cut(s)	1204, 1600
TspDTI	ATGAA	7 cut(s)	273, 525, 723, 1529, 1580, 1763, 2121
TspGWI	ACGGA	1 cut(s)	366
TspRI	CASTG	7 cut(s)	193, 825, 1150, 1299, 1672, 1899, 2062
VpaK11BI	GGWCC	3 cut(s)	663, 1100, 1706
XapI	RAATTY	3 cut(s)	1619, 1774, 1820
XceI	RCATGY	2 cut(s)	1120, 1274
XcmI	CCANNNNNNNNNTGG	1 cut(s)	486
XmnI	GAANNNNTTC	2 cut(s)	232, 1240
XspI	CTAG	2 cut(s)	1400, 1701
Zsp2I	ATGCAT	2 cut(s)	592, 2011

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.