Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0002994

Physical Location & Seq

Genomic Coordinates Forward (+)

17937622 .. 17941292

Gene Structure 3671 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G44530.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2523 bp

ATGGTTAGAGTAATGTTGGTGAGGTTTGGGTTTTTATTACTTATGATCTCTTTTGTGTTTTTGTCTAATAACACTTTGGGACAACAACAAGACGATGATGATGATTCTGCTGTGTACATTGTCACACTTAAACAACCTCCTATTGTCCATCTCTTTGAAGAACAAGAGCTTAAACACAAAAAGTCAAAGTTTACACCAAAATTGCGACCAAGGAACAATTCAAGGAAACGTCATGGGAAGTCAAAGATACCATCTGTTGTTCAATCTCATGACTCTTTCTTGAGAAAGACTCTAAAAGGAGAGAAGTATATAAAGCTTTATAGTTACCATTATCTTATCAATGGATTTGCTCTGTTTATTAACTCACAACAGGCTGAGAAGCTTTCAATGAGAAAAGAAGTAGCAAACATAGTGTTGGATTACTCTGTTAGGACAGCAACAACTTATACTCCACAGTTTATGGGTTTACCACAAGGAGCATGGGTCAAAGAAGGTGGATTTGAGATTGCTGGAGAAGGAGTTATAATCGGTTTTATCGATACTGGGATCGATCCGAATCATCCTAGTTTCAATGACAATGACTCTAAGCGTTCATATCCAATCCCTAAGCATTTCTCAGGTGTTTGTGAAGTTACACCGGATTTTCCATCAGGATCTTGCAATAAGAAGCTTATTGGAGCACGGCATTTCGCGCAATCCGCTGTAACCAGAGGAATCTTTAACTCATCTGAAGATTACGCTTCTCCTTTCGATGGAGATGGACATGGAACACACACAGCTTCGGTTGCAGCGGGTAACCACGGAGTTCCAGTGATAGTTTCTAACCATAACTTTGGATACGCTAGTGGAATCGCTCCTCGTGCATTTATTTCTGTTTACAAGGCATTGTATAAGAGTTTTGGAGGTTTTGCTGCAGATGTTGTAGCAGCTATAGATCAGGCAGCTCAAGATGGAGTAGATATATTAAGCCTATCAATTACACCGAATCGGAAACCTCCCGGTGTTGCCACTTTCTTTAATCCTATCGACATGGCATTACTTTCCGCTGTAAAAGCCGGAATCTTCGTAGTCCAAGCTGCGGGAAATACCGGTCCAGCGCCTAAAACCATGTCTTCTTTTAGTCCTTGGATATTCACCGTTGGTGCTTCTTCTCATGATAGAGTTTACTCTAATTCTTTAACCTTAGGAAACAATGTAACTATTCCAGGCATGGGATTCGCAATTCCTACAGATAGTGGAAAAATGTACAAGATGATATCTGCTTTTCATGCCTTGAACAATAGTACTTCTGTAGATAAAGATATGTATGTAGGTGAATGTCAAGATTATGAAAACTTCGATCAAGATCGTGTCTCAGGGAAACTTTTGATATGTAGCTACTCTGCTCGTTTTGTTCTCGGGCTTTCGACTATAAAACAAGCTTTAGATGTTGCCAAGAATCTATCCGCGACTGGTGTGATATTCTATATAGACCCGTATGTTCTTGGTTTTGAGATCAATCCAACACCGATGGATATGCCCGGGATCATAATTCCATCTGTAGAAGATTCCAAGACTTTACTCAAGTACTATAACTCTTCTATTCAAAGAGATGTAACCACTAAAGAAATTGTTAGTTTTGGAGCGGTTGCAGCCATAGAAGGCGGTTTAAATGCTAACTTTAGTAACAGAGCTCCTAAGGTAATGTATTACTCAGCAAGAGGACCTGATCCTGAAGACAACTCTTTTAACGACGCAGACGTATTAAAACCGAACCTCGTAGCACCTGGAAACTCTATTTGGGGTGCTTGGAGTTCTGCTTCAACGGATTCTACCGAGTTTGAAGGTGAGAAATTTGCGATGATGTCCGGTACAAGTATGGCTGCTCCTCATGTAGCTGGTGTGGCTGCATTAATCAAACAAAGTTATCCACAGTTTACTCCTTCAACAATCTCATCCGCGCTTTCAACAACGGCTCTCCTAAATGATAATAAAGGCAGTCCGATAATGGCTCAGCGAACTTATTCCAATCCCGATCAAAGCCTCTATACCGCAACACCGTCTGATATGGGGAGCGGTTTTGTTAACGCAACGGCAGCTTTAGACCCTGGTCTAGTTTTTGATACAAGTTTTGAAGATTATATATCATTTCTTTGTGGGATCAACGGCTCGGATACGGTGGTATTCAACTACACCGGATTCCGCTGTCCCGCTAACAACACACCAGTCAGTGGTTTTGACCTCAATTTGCCATCAATTACAGTATCGACACTTAGTGGCACACAAACTTTCCAAAGATCGATGAGAAACATAGCCGGCAATGAGACATACAATGTTGGTTGGAGTCCTCCTTATGGTGTTTCAATGAAAGTATCACCTACTCAATTTTCTATTGCTATGGGAGAAAATCAAGTACTTAGCGTAACCCTCACGGTGACAAAGAACAGTTCCAGTTCTAGTTTTGGCAGAATCGGATTGTTTGGAAATACAGGACACATTGTTAATATTCCTGTAACTGTCATAGCGAAAATCGCTTCGAGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004252 GO:0005575 GO:0005618 GO:0005623 GO:0005911 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0009505 GO:0009506 GO:0016787 GO:0017171 GO:0019538 GO:0030054 GO:0030312 GO:0043170 GO:0044238 GO:0044464 GO:0055044 GO:0070011 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 Peptidase_S8

Protein Analysis

840

Amino Acids

90.62

Weight (kDa)

6.77

Isoelectric Point (pI)

33.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I9	PF05922	38 - 145	6.1e-14	Peptidase inhibitor I9
Peptidase_S8	PF00082	171 - 657	3.7e-48	Subtilase family
fn3_6	PF17766	765 - 858	4.3e-16	Fibronectin type-III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002994)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G30600 AT4G20430 AT4G20430 AT4G20430 AT5G44530 AT5G44530 AT5G44530
fragaria_vesca	FvH4_3g11550 FvH4_3g11550 FvH4_3g11550 FvH4_3g11550 FvH4_3g11550 FvH4_3g11550
malus_domestica	MD00G1096900.v1.1 MD10G1240800.v1.1
prunus_persica	Prupe.4G102800_v2.0.a1 Prupe.4G102800_v2.0.a1 Prupe.4G102800_v2.0.a1 Prupe.4G102800_v2.0.a1 Prupe.4G102800_v2.0.a1 Prupe.4G102800_v2.0.a1 Prupe.4G102800_v2.0.a1
pyrus_communis	pycom05g24650 pycom05g24660 pycom10g20150
rosa_chinensis	RchiOBHm_Chr5g0018491
rosa_laevigata	RLG00000032371
rosa_multiflora	Rmu_sc0002579.1_g000001 Rmu_sc0004464.1_g000018 Rmu_sc0038967.1_g000001
rosa_roxburghii	Rroxscaffold_1G00059240
rosa_rugosa	Rorug05G0044600 Rorug05G0044700
rosa_samantha	Rh5AG135800 Rh5BG134700 Rh5CG146800 Rh5DG135900
rosa_wichuraiana	Rw5G012030

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	524
AccB7I	CCANNNNNTGG	1 cut(s)	2210
AccBSI	CCGCTC	2 cut(s)	1625, 2055
AccII	CGCG	3 cut(s)	692, 1448, 1940
AclWI	GGATC	6 cut(s)	545, 554, 661, 1532, 1703, 2147
AcsI	RAATTY	1 cut(s)	1832
AcuI	CTGAAG	2 cut(s)	750, 1734
AdeI	CACNNNGTG	1 cut(s)	2255
AfaI	GTAC	6 cut(s)	116, 1247, 1285, 1568, 1852, 2394
AfiI	CCNNNNNNNGG	5 cut(s)	752, 1078, 1211, 2210, 2333
AgeI	ACCGGT	1 cut(s)	1088
AjnI	CCWGG	3 cut(s)	1204, 1765, 2086
AjuI	GAANNNNNNNTTGG	4 cut(s)	202, 234, 1762, 1794
Alw21I	GWGCWC	2 cut(s)	682, 1675
Alw26I	GTCTC	2 cut(s)	1357, 2297
AlwI	GGATC	6 cut(s)	545, 554, 661, 1532, 1703, 2147
Ama87I	CYCGRG	2 cut(s)	1397, 1520
ApeKI	GCWGC	9 cut(s)	788, 911, 926, 941, 1076, 1631, 1862, 1886, 2075
ApoI	RAATTY	1 cut(s)	1832
AseI	ATTAAT	1 cut(s)	1892
AsiGI	ACCGGT	1 cut(s)	1088
Asp700I	GAANNNNTTC	1 cut(s)	2002
AspLEI	GCGC	3 cut(s)	694, 1099, 1942
AspS9I	GGNCC	2 cut(s)	1091, 1703
AsuC2I	CCSGG	3 cut(s)	999, 1521, 1522
AsuHPI	GGTGA	6 cut(s)	31, 1126, 1325, 1838, 2346, 2425
AvaI	CYCGRG	2 cut(s)	1397, 1520
AvaII	GGWCC	2 cut(s)	1091, 1703
AxyI	CCTNAGG	2 cut(s)	1183, 1677
BaeI	ACNNNNGTAYC	4 cut(s)	829, 862, 2145, 2178
BanII	GRGCYC	1 cut(s)	1675
BauI	CACGAG	1 cut(s)	858
BbsI	GAAGAC	2 cut(s)	1104, 1722
Bbv12I	GWGCWC	2 cut(s)	682, 1675
BbvI	GCAGC	9 cut(s)	800, 898, 938, 953, 1063, 1643, 1849, 1873, 2087
BccI	CCATC	9 cut(s)	156, 259, 655, 746, 752, 944, 1504, 1543, 2239
BceAI	ACGGC	4 cut(s)	698, 1968, 2088, 2161
BcgI	CGANNNNNNTGC	4 cut(s)	1198, 1232, 1498, 1532
BciT130I	CCWGG	3 cut(s)	1206, 1767, 2088
BciVI	GTATCC	2 cut(s)	830, 2146
BcnI	CCSGG	3 cut(s)	999, 1521, 1522
BcoDI	GTCTC	2 cut(s)	1357, 2297
BfaI	CTAG	4 cut(s)	564, 843, 2093, 2436
BfmI	CTRYAG	5 cut(s)	912, 930, 1227, 1290, 1539
BfoI	RGCGCY	1 cut(s)	1100
BfuI	GTATCC	2 cut(s)	830, 2146
BisI	GCNGC	9 cut(s)	789, 912, 927, 942, 1077, 1632, 1863, 1887, 2076
BlpI	GCTNAGC	1 cut(s)	1992
BlsI	GCNGC	9 cut(s)	790, 913, 928, 943, 1078, 1633, 1864, 1888, 2077
BmcAI	AGTACT	3 cut(s)	1285, 1568, 2394
Bme1390I	CCNGG	6 cut(s)	999, 1206, 1521, 1522, 1767, 2088
Bme18I	GGWCC	2 cut(s)	1091, 1703
BmeT110I	CYCGRG	2 cut(s)	1397, 1520
BmgT120I	GGNCC	2 cut(s)	1091, 1703
BmrFI	CCNGG	6 cut(s)	999, 1206, 1521, 1522, 1767, 2088
BmrI	ACTGGG	1 cut(s)	552
BmuI	ACTGGG	1 cut(s)	552
BoxI	GACNNNNGTC	1 cut(s)	2088
BpiI	GAAGAC	2 cut(s)	1104, 1722
BplI	GAGNNNNNCTC	2 cut(s)	274, 306
BpmI	CTGGAG	1 cut(s)	531
Bpu10I	CCTNAGC	1 cut(s)	606
Bpu1102I	GCTNAGC	1 cut(s)	1992
BpuEI	CTTGAG	3 cut(s)	301, 930, 1547
BpuMI	CCSGG	3 cut(s)	999, 1521, 1522
Bsa29I	ATCGAT	3 cut(s)	537, 549, 2279
BsaBI	GATNNNNATC	2 cut(s)	555, 1344
BsaJI	CCNNGG	5 cut(s)	209, 799, 1124, 1520, 2086
BsaWI	WCCGGW	4 cut(s)	637, 1088, 1847, 2174
BsaXI	ACNNNNNCTCC	8 cut(s)	433, 463, 468, 498, 504, 534, 795, 825
Bsc4I	CCNNNNNNNGG	5 cut(s)	752, 1078, 1211, 2210, 2333
Bse118I	RCCGGY	2 cut(s)	1088, 2294
Bse1I	ACTGG	5 cut(s)	547, 809, 1456, 2204, 2430
Bse21I	CCTNAGG	2 cut(s)	1183, 1677
Bse3DI	GCAATG	1 cut(s)	2305
Bse8I	GATNNNNATC	2 cut(s)	555, 1344
BseBI	CCWGG	3 cut(s)	1206, 1767, 2088
BseCI	ATCGAT	3 cut(s)	537, 549, 2279
BseDI	CCNNGG	5 cut(s)	209, 799, 1124, 1520, 2086
BseGI	GGATG	2 cut(s)	559, 1934
BseJI	GATNNNNATC	2 cut(s)	555, 1344
BseLI	CCNNNNNNNGG	5 cut(s)	752, 1078, 1211, 2210, 2333
BseMI	GCAATG	1 cut(s)	2305
BseMII	CTCAG	5 cut(s)	366, 630, 1368, 1707, 2006
BseNI	ACTGG	5 cut(s)	547, 809, 1456, 2204, 2430
BseRI	GAGGAG	2 cut(s)	846, 1857
BseXI	GCAGC	9 cut(s)	800, 898, 938, 953, 1063, 1643, 1849, 1873, 2087
Bsh1236I	CGCG	3 cut(s)	692, 1448, 1940
BshTI	ACCGGT	1 cut(s)	1088
BshVI	ATCGAT	3 cut(s)	537, 549, 2279
BsiHKAI	GWGCWC	2 cut(s)	682, 1675
BsiHKCI	CYCGRG	2 cut(s)	1397, 1520
BsiSI	CCGG	8 cut(s)	638, 999, 1056, 1089, 1521, 1848, 2175, 2295
BslFI	GGGAC	2 cut(s)	93, 2172
BslI	CCNNNNNNNGG	5 cut(s)	752, 1078, 1211, 2210, 2333
BsmAI	GTCTC	2 cut(s)	1357, 2297
BsmFI	GGGAC	2 cut(s)	93, 2172
BsoBI	CYCGRG	2 cut(s)	1397, 1520
Bsp1286I	GDGCHC	2 cut(s)	682, 1675
Bsp1407I	TGTACA	2 cut(s)	114, 1245
Bsp1720I	GCTNAGC	1 cut(s)	1992
BspCNI	CTCAG	5 cut(s)	367, 629, 1367, 1706, 2005
BspDI	ATCGAT	3 cut(s)	537, 549, 2279
BspFNI	CGCG	3 cut(s)	692, 1448, 1940
BspHI	TCATGA	2 cut(s)	268, 1153
BspMAI	CTGCAG	1 cut(s)	916
BspPI	GGATC	6 cut(s)	545, 554, 661, 1532, 1703, 2147
BsrBI	CCGCTC	2 cut(s)	1625, 2055
BsrDI	GCAATG	1 cut(s)	2305
BsrFI	RCCGGY	2 cut(s)	1088, 2294
BsrGI	TGTACA	2 cut(s)	114, 1245
BsrI	ACTGG	5 cut(s)	547, 809, 1456, 2204, 2430
BssAI	RCCGGY	2 cut(s)	1088, 2294
BssECI	CCNNGG	5 cut(s)	209, 799, 1124, 1520, 2086
BssSI	CACGAG	1 cut(s)	858
BssT1I	CCWWGG	2 cut(s)	209, 1124
Bst2BI	CACGAG	1 cut(s)	858
Bst2UI	CCWGG	3 cut(s)	1206, 1767, 2088
Bst4CI	ACNGT	9 cut(s)	456, 1138, 1914, 2040, 2158, 2242, 2413, 2426, 2497
Bst6I	CTCTTC	1 cut(s)	1582
BstAUI	TGTACA	2 cut(s)	114, 1245
BstC8I	GCNNGC	1 cut(s)	2296
BstDSI	CCRYGG	1 cut(s)	799
BstEII	GGTNACC	1 cut(s)	794
BstF5I	GGATG	2 cut(s)	559, 1934
BstFNI	CGCG	3 cut(s)	692, 1448, 1940
BstH2I	RGCGCY	1 cut(s)	1100
BstHHI	GCGC	3 cut(s)	694, 1099, 1942
BstMAI	GTCTC	2 cut(s)	1357, 2297
BstNI	CCWGG	3 cut(s)	1206, 1767, 2088
BstPAI	GACNNNNGTC	1 cut(s)	2088
BstPI	GGTNACC	1 cut(s)	794
BstSCI	CCNGG	6 cut(s)	997, 1204, 1519, 1520, 1765, 2086
BstSFI	CTRYAG	5 cut(s)	912, 930, 1227, 1290, 1539
BstUI	CGCG	3 cut(s)	692, 1448, 1940
BstV1I	GCAGC	9 cut(s)	800, 898, 938, 953, 1063, 1643, 1849, 1873, 2087
BstV2I	GAAGAC	2 cut(s)	1104, 1722
BstX2I	RGATCY	1 cut(s)	653
BstXI	CCANNNNNNTGG	1 cut(s)	833
BstYI	RGATCY	1 cut(s)	653
Bsu15I	ATCGAT	3 cut(s)	537, 549, 2279
Bsu36I	CCTNAGG	2 cut(s)	1183, 1677
BsuI	GTATCC	2 cut(s)	830, 2146
BsuTUI	ATCGAT	3 cut(s)	537, 549, 2279
BtgI	CCRYGG	1 cut(s)	799
BtgZI	GCGATG	1 cut(s)	1853
BtsCI	GGATG	2 cut(s)	559, 1934
BtsIMutI	CAGTG	2 cut(s)	816, 2215
Cac8I	GCNNGC	1 cut(s)	2296
CciI	TCATGA	2 cut(s)	268, 1153
CfoI	GCGC	3 cut(s)	694, 1099, 1942
Cfr10I	RCCGGY	2 cut(s)	1088, 2294
Cfr13I	GGNCC	2 cut(s)	1091, 1703
Cfr9I	CCCGGG	1 cut(s)	1520
ClaI	ATCGAT	3 cut(s)	537, 549, 2279
CseI	GACGC	1 cut(s)	1742
Csp6I	GTAC	6 cut(s)	115, 1246, 1284, 1567, 1851, 2393
CspAI	ACCGGT	1 cut(s)	1088
CspCI	CAANNNNNGTGG	2 cut(s)	475, 510
CviQI	GTAC	6 cut(s)	115, 1246, 1284, 1567, 1851, 2393
DraI	TTTAAA	1 cut(s)	1650
DraIII	CACNNNGTG	1 cut(s)	2255
Eam1104I	CTCTTC	1 cut(s)	1582
EarI	CTCTTC	1 cut(s)	1582
Ecl136II	GAGCTC	1 cut(s)	1673
Eco130I	CCWWGG	2 cut(s)	209, 1124
Eco24I	GRGCYC	1 cut(s)	1675
Eco32I	GATATC	1 cut(s)	1257
Eco47I	GGWCC	2 cut(s)	1091, 1703
Eco53kI	GAGCTC	1 cut(s)	1673
Eco57I	CTGAAG	2 cut(s)	750, 1734
Eco81I	CCTNAGG	2 cut(s)	1183, 1677
Eco88I	CYCGRG	2 cut(s)	1397, 1520
Eco91I	GGTNACC	1 cut(s)	794
EcoICRI	GAGCTC	1 cut(s)	1673
EcoO109I	RGGNCCY	1 cut(s)	1703
EcoO65I	GGTNACC	1 cut(s)	794
EcoRII	CCWGG	3 cut(s)	1204, 1765, 2086
EcoRV	GATATC	1 cut(s)	1257
EcoT14I	CCWWGG	2 cut(s)	209, 1124
EcoT38I	GRGCYC	1 cut(s)	1675
ErhI	CCWWGG	2 cut(s)	209, 1124
FaqI	GGGAC	2 cut(s)	93, 2172
FauI	CCCGC	3 cut(s)	784, 1072, 2197
Fnu4HI	GCNGC	9 cut(s)	789, 912, 927, 942, 1077, 1632, 1863, 1887, 2076
FokI	GGATG	2 cut(s)	546, 1921
FriOI	GRGCYC	1 cut(s)	1675
Fsp4HI	GCNGC	9 cut(s)	789, 912, 927, 942, 1077, 1632, 1863, 1887, 2076
FspBI	CTAG	4 cut(s)	564, 843, 2093, 2436
GlaI	GCGC	3 cut(s)	693, 1098, 1941
GluI	GCNGC	9 cut(s)	789, 912, 927, 942, 1077, 1632, 1863, 1887, 2076
GsuI	CTGGAG	1 cut(s)	531
HaeII	RGCGCY	1 cut(s)	1100
HapII	CCGG	8 cut(s)	638, 999, 1056, 1089, 1521, 1848, 2175, 2295
HgaI	GACGC	1 cut(s)	1742
HhaI	GCGC	3 cut(s)	694, 1099, 1942
Hin6I	GCGC	3 cut(s)	692, 1097, 1940
HinP1I	GCGC	3 cut(s)	692, 1097, 1940
HincII	GTYRAC	1 cut(s)	2065
HindII	GTYRAC	1 cut(s)	2065
HindIII	AAGCTT	4 cut(s)	314, 380, 668, 1419
HpaI	GTTAAC	1 cut(s)	2065
HpaII	CCGG	8 cut(s)	638, 999, 1056, 1089, 1521, 1848, 2175, 2295
HphI	GGTGA	6 cut(s)	31, 1126, 1325, 1838, 2346, 2425
Hpy166II	GTNNAC	7 cut(s)	115, 192, 467, 877, 1165, 1917, 2065
Hpy188I	TCNGA	7 cut(s)	555, 730, 990, 1983, 2044, 2152, 2453
Hpy188III	TCNNGA	9 cut(s)	269, 280, 651, 947, 1154, 1322, 1343, 1712, 2012
Hpy8I	GTNNAC	7 cut(s)	115, 192, 467, 877, 1165, 1917, 2065
Hpy99I	CGWCG	1 cut(s)	1736
HpyAV	CCTTC	5 cut(s)	485, 509, 1634, 1817, 1932
HpyCH4III	ACNGT	9 cut(s)	456, 1138, 1914, 2040, 2158, 2242, 2413, 2426, 2497
HpyCH4IV	ACGT	2 cut(s)	229, 1740
HpyCH4V	TGCA	6 cut(s)	660, 788, 863, 914, 1631, 1889
HpySE526I	ACGT	2 cut(s)	229, 1740
HspAI	GCGC	3 cut(s)	692, 1097, 1940
KroI	GCCGGC	1 cut(s)	2294
KroNI	GCCGGC	1 cut(s)	2296
KspAI	GTTAAC	1 cut(s)	2065
LmnI	GCTCC	7 cut(s)	476, 677, 859, 1622, 1678, 1870, 2052
Lsp1109I	GCAGC	9 cut(s)	800, 898, 938, 953, 1063, 1643, 1849, 1873, 2087
MaeI	CTAG	4 cut(s)	564, 843, 2093, 2436
MaeII	ACGT	2 cut(s)	229, 1740
MbiI	CCGCTC	2 cut(s)	1625, 2055
MboII	GAAGA	9 cut(s)	170, 743, 1054, 1104, 1140, 1556, 1569, 1727, 2126
MflI	RGATCY	1 cut(s)	653
MhlI	GDGCHC	2 cut(s)	682, 1675
MlyI	GAGTC	4 cut(s)	266, 283, 575, 2332
MmeI	TCCRAC	3 cut(s)	396, 1526, 2300
MroNI	GCCGGC	1 cut(s)	2294
MroXI	GAANNNNTTC	1 cut(s)	2002
MspA1I	CMGCKG	4 cut(s)	701, 791, 1046, 2184
MspI	CCGG	8 cut(s)	638, 999, 1056, 1089, 1521, 1848, 2175, 2295
MspR9I	CCNGG	6 cut(s)	999, 1206, 1521, 1522, 1767, 2088
MvaI	CCWGG	3 cut(s)	1206, 1767, 2088
MvnI	CGCG	3 cut(s)	692, 1448, 1940
NaeI	GCCGGC	1 cut(s)	2296
NciI	CCSGG	3 cut(s)	999, 1521, 1522
NgoMIV	GCCGGC	1 cut(s)	2294
NmuCI	GTSAC	2 cut(s)	121, 2413
PagI	TCATGA	2 cut(s)	268, 1153
PcsI	WCGNNNNNNNCGW	2 cut(s)	534, 1737
PdiI	GCCGGC	1 cut(s)	2296
PdmI	GAANNNNTTC	1 cut(s)	2002
PflMI	CCANNNNNTGG	1 cut(s)	2210
PinAI	ACCGGT	1 cut(s)	1088
PkrI	GCNGC	9 cut(s)	790, 913, 928, 943, 1078, 1633, 1864, 1888, 2077
PleI	GAGTC	4 cut(s)	266, 283, 575, 2331
PpsI	GAGTC	4 cut(s)	266, 283, 575, 2331
PpuMI	RGGWCCY	1 cut(s)	1703
PshAI	GACNNNNGTC	1 cut(s)	2088
PshBI	ATTAAT	1 cut(s)	1892
PsiI	TTATAA	1 cut(s)	524
Psp124BI	GAGCTC	1 cut(s)	1675
Psp5II	RGGWCCY	1 cut(s)	1703
Psp6I	CCWGG	3 cut(s)	1204, 1765, 2086
PspEI	GGTNACC	1 cut(s)	794
PspGI	CCWGG	3 cut(s)	1204, 1765, 2086
PspPI	GGNCC	2 cut(s)	1091, 1703
PspPPI	RGGWCCY	1 cut(s)	1703
PstI	CTGCAG	1 cut(s)	916
PsuI	RGATCY	1 cut(s)	653
RsaI	GTAC	6 cut(s)	116, 1247, 1285, 1568, 1852, 2394
RsaNI	GTAC	6 cut(s)	115, 1246, 1284, 1567, 1851, 2393
SacI	GAGCTC	1 cut(s)	1675
SatI	GCNGC	9 cut(s)	789, 912, 927, 942, 1077, 1632, 1863, 1887, 2076
Sau96I	GGNCC	2 cut(s)	1091, 1703
ScaI	AGTACT	3 cut(s)	1285, 1568, 2394
SchI	GAGTC	4 cut(s)	266, 283, 575, 2332
ScrFI	CCNGG	6 cut(s)	999, 1206, 1521, 1522, 1767, 2088
SduI	GDGCHC	2 cut(s)	682, 1675
SfcI	CTRYAG	5 cut(s)	912, 930, 1227, 1290, 1539
SinI	GGWCC	2 cut(s)	1091, 1703
SmaI	CCCGGG	1 cut(s)	1522
SmlI	CTYRAG	3 cut(s)	280, 945, 1562
SmoI	CTYRAG	3 cut(s)	280, 945, 1562
SspI	AATATT	1 cut(s)	2485
SspMI	CTAG	4 cut(s)	564, 843, 2093, 2436
SstI	GAGCTC	1 cut(s)	1675
StyD4I	CCNGG	6 cut(s)	997, 1204, 1519, 1520, 1765, 2086
StyI	CCWWGG	2 cut(s)	209, 1124
TaaI	ACNGT	9 cut(s)	456, 1138, 1914, 2040, 2158, 2242, 2413, 2426, 2497
TaiI	ACGT	2 cut(s)	232, 1743
TaqI	TCGA	9 cut(s)	537, 549, 750, 1026, 1338, 1406, 2246, 2279, 2516
TatI	WGTACW	5 cut(s)	114, 1245, 1283, 1566, 2392
TscAI	CASTG	2 cut(s)	816, 2215
TseFI	GTSAC	2 cut(s)	121, 2413
TseI	GCWGC	9 cut(s)	788, 911, 926, 941, 1076, 1631, 1862, 1886, 2075
Tsp45I	GTSAC	2 cut(s)	121, 2413
TspDTI	ATGAA	4 cut(s)	582, 1256, 1344, 2360
TspGWI	ACGGA	2 cut(s)	816, 1820
TspMI	CCCGGG	1 cut(s)	1520
TspRI	CASTG	2 cut(s)	816, 2215
Van91I	CCANNNNNTGG	1 cut(s)	2210
VpaK11BI	GGWCC	2 cut(s)	1091, 1703
VspI	ATTAAT	1 cut(s)	1892
XapI	RAATTY	1 cut(s)	1832
XcmI	CCANNNNNNNNNTGG	1 cut(s)	477
XmaI	CCCGGG	1 cut(s)	1520
XmnI	GAANNNNTTC	1 cut(s)	2002
XspI	CTAG	4 cut(s)	564, 843, 2093, 2436
ZrmI	AGTACT	3 cut(s)	1285, 1568, 2394

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.