Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0002994

Physical Location & Seq

Genomic Coordinates Forward (+)

33630939 .. 33637606

Gene Structure 6668 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1240800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2541 bp

ATGGGAAGCTTTCATGGGGTGCTTTTAGTGGGATTGTTTTTTTTGGGGATGCTTGTGAGTTCTTTGTGTCAAGACGAAGATTCTGATAACTTCTCGGCTGTTTACATCGTCACTCTCAGAGAAGCTCCGGCCGTTCACTACGATGCCGAGTTGAGAAGGAGCAGTATAAATGGTGTCAGACATGGCGGTGCTTCTGAGAGGTTGAACATTCACAAACCAAGGTTTAGAAATATTTCGAGAACAGATCGTAGGTATAGCTCTTATATCGCTCGGGTTCATGATTCATTATTGAGGAGAGTGTTCAGAGGGGAAAAATATCTGAAGCTGTACAGCTACCACTACTTGATAAACGGGTTTGCTGTGTTTGTTACCCAAGATCAGGTTGATAAGCTTTCGAGGAGGAGAGAAGTAGCAAATGTGGTTTTGGACTTTTCTGTTAGAACTGCAACCACGCATACTCCTCGGTTCTTGGGTTTACCACAAGGAGCATGGGTTCAAGGAGGAGGATATGAATCAGCTGGAGAAGGGGTAGTTATTGGGTTTATTGATACTGGCATTGATCCAACTCACAGTAGTTTTGATGACCATACATCTGAACGTCCATATCCAGTTCCAGCCCATTTTTCTGGCATCTGTGAGGTTACTCCGGATTTCCCATCTGGGTCCTGCAATAGGAAGCTTATTGCAGCTCGCCATTTTGCATCATCTGCTATAACCAGAGGCGTTTTCAATTCATCTCAGGACTACGCTTCTCCCTTTGACGGAGAAGGTCATGGCACGCACACCGCTTCCATTGCTGCAGGAAACCATGGAATTCGAGTGGTTGTTGCTGGGCATCATTTCGGAAATGCAAGTGGGATGGCTCCTCGTTCACACATCGCTGTTTACAAGGCATTGTACAAAAGCTTTGGAGGCTTTGCTGCTGATGTTGTTGCTGCCATAGACCAGGCTGCTCAGGATGGGGTTGACATAATAAGCTTATCAATCACTCCCAACCGGCGTCCTCCCGGAATTGCAACATTTTTCAATCCCATAGACATGGCATTACTATCAGCTGTAAAGGCTGGTATATTTGTTGTCCAAGCAGCAGGAAATACGGGACCATCACCAAAGAGCATGTCTTCCTTCAGTCCATGGATATTCACCGTTGGCTCTTCCTCTCATGACAGAAACTACAGCAATTCTATAATGCTTGGCAACAATGTCACCATTACTGGAGTTGGACTTGCACCGGGAACAGATACGATGTACACTCTGATTTCTGCCACACATGCTTTGAACAATGGCACAACTGTGTCAGATGATATGTATGTTGGTGAATGCCAAGACTCGAGCAACTTTAATCGGGATTTGGTCCGGGGGAACCTCTTGATCTGCAGCTACTCAATCCGATTTGTGCTAGGGATCTCCACAATCCATAAATCCTTAGAAACAGCCAAAAACCTAAGTGCAATTGGTGTTGTGTTCACCATGGATGCTTTTGTCATTGGTTTTCAGCTTAACCCTACTCCAATGAAATTACCCGGCATCATAATTCCATCCCCAGAGGATTCCAAGGTTTTACTCAAATACTACAATCTTTCCTTGGAGAGAGATATCATGACAAAGAGAATTACCAAATTTGGGGCACTTGCAACCATTTGTGGAGGTTTGAAAGCAAACTACAGTACTTCTGCTCCAAAGATCATGTATTACTCTGCTAGAGGTCCAGATCCGGAAGACAATTTTCTAGACGATGCAGATATAATGAAACCCAACTTGGTAGCTCCTGGAAATTCTATATGGGCTGCTTGGAGTTCTGTTGGGCAAGACTCGATTGAATTTCAAGGTGAGCACTTCGCAATGATGTCCGGGACAAGCATGGCTGCTCCTCATGTTGCTGGGCTTGCTGCACTGGTTAGGCAGAAGTTCCCCAATTTCAGTCCTTCTGCCATTGCATCTGCACTTTCCACAACAGCTTCTCTATATGACAGAAATGGTGGGCCCATAATGGCTCAGCGTGCTTATGCATTTCCAGATCAAAACCAGTCTCCAGCAACACCCTTTGACATGGGCAGTGGTTTTGTAAATGCTACAGCAGCGCTGAATCCAGGGTTGATTTTTGATACCAGTTATGACAATTATATGTCGTTTCTTTGTGGCATCAATGGATCGGCTCCTGTGGTCTTGAATTACACCGGTGAGAGCTGTTGGACGTATAATTCTACCATCAATGGCGGTGACCTGAACTTGCCATCAATAACAATAGCAAAACTAAACCAGTCTAGAACGGTGCTGAGAACAGTGAGGAACGTTGCTGCAAATGAAACCTATAGCGTTGGCTGGAGTGCTCCTTTCGGAGTTTCATTGAAGGTGTCTCCACTTCATTTTTCGATTGCCAGTGGGGAGACACAGGTCTTAAGTGTATTCGTTAATGCTACGAGTAACAGCACAACTGCTAGCTATGGGAGGATCGGACTATTTGGACATCTAAGTCATGTTGTCAACATTCCATTGTCAGTCATTGTCAAAATCACATATAATACTACTGCCAACGGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004252 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0016787 GO:0017171 GO:0019538 GO:0043170 GO:0044238 GO:0070011 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 Peptidase_S8

Protein Analysis

847

Amino Acids

90.95

Weight (kDa)

7.7

Isoelectric Point (pI)

36.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I9	PF05922	34 - 148	1.5e-12	Peptidase inhibitor I9
Peptidase_S8	PF00082	174 - 666	1.7e-45	Subtilase family
fn3_6	PF17766	741 - 834	1.2e-14	Fibronectin type-III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002994)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G30600 AT4G20430 AT4G20430 AT4G20430 AT5G44530 AT5G44530 AT5G44530
fragaria_vesca	FvH4_3g11550 FvH4_3g11550 FvH4_3g11550 FvH4_3g11550 FvH4_3g11550 FvH4_3g11550
malus_domestica	MD00G1096900.v1.1 MD10G1240800.v1.1
prunus_persica	Prupe.4G102800_v2.0.a1 Prupe.4G102800_v2.0.a1 Prupe.4G102800_v2.0.a1 Prupe.4G102800_v2.0.a1 Prupe.4G102800_v2.0.a1 Prupe.4G102800_v2.0.a1 Prupe.4G102800_v2.0.a1
pyrus_communis	pycom05g24650 pycom05g24660 pycom10g20150
rosa_chinensis	RchiOBHm_Chr5g0018491
rosa_laevigata	RLG00000032371
rosa_multiflora	Rmu_sc0002579.1_g000001 Rmu_sc0004464.1_g000018 Rmu_sc0038967.1_g000001
rosa_roxburghii	Rroxscaffold_1G00059240
rosa_rugosa	Rorug05G0044600 Rorug05G0044700
rosa_samantha	Rh5AG135800 Rh5BG134700 Rh5CG146800 Rh5DG135900
rosa_wichuraiana	Rw5G012030

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2472
AccIII	TCCGGA	2 cut(s)	646, 1714
AciI	CCGC	3 cut(s)	186, 786, 2217
AclI	AACGTT	1 cut(s)	2292
AclWI	GGATC	5 cut(s)	554, 1412, 1706, 2158, 2459
AcoI	YGGCCR	1 cut(s)	129
AcsI	RAATTY	4 cut(s)	813, 1619, 1774, 1820
AcuI	CTGAAG	2 cut(s)	341, 1111
AcyI	GRCGYC	1 cut(s)	1000
AfaI	GTAC	4 cut(s)	329, 899, 1250, 1669
AfeI	AGCGCT	1 cut(s)	2082
AfiI	CCNNNNNNNGG	4 cut(s)	379, 672, 761, 1623
AflII	CTTAAG	1 cut(s)	2398
AgeI	ACCGGT	1 cut(s)	2177
AjnI	CCWGG	3 cut(s)	945, 1768, 2089
AjuI	GAANNNNNNNTTGG	2 cut(s)	1742, 1774
AleI	CACNNNNGTG	1 cut(s)	1292
AloI	GAACNNNNNNTCC	2 cut(s)	477, 509
Alw21I	GWGCWC	2 cut(s)	1836, 2332
Alw26I	GTCTC	3 cut(s)	2034, 2361, 2381
AlwI	GGATC	5 cut(s)	554, 1412, 1706, 2158, 2459
Ama87I	CYCGRG	2 cut(s)	270, 1330
Aor13HI	TCCGGA	2 cut(s)	646, 1714
Aor51HI	AGCGCT	1 cut(s)	2082
AoxI	GGCC	2 cut(s)	129, 1982
ApaI	GGGCCC	1 cut(s)	1986
ApoI	RAATTY	4 cut(s)	813, 1619, 1774, 1820
ArsI	GACNNNNNNTTYG	2 cut(s)	1103, 1135
AsiGI	ACCGGT	1 cut(s)	2177
Asp700I	GAANNNNTTC	1 cut(s)	232
AspLEI	GCGC	1 cut(s)	2083
AspS9I	GGNCC	6 cut(s)	663, 1100, 1354, 1706, 1982, 1983
AsuC2I	CCSGG	5 cut(s)	1008, 1233, 1358, 1524, 1852
AsuHPI	GGTGA	8 cut(s)	1098, 1135, 1198, 1328, 1459, 1841, 2192, 2231
AsuNHI	GCTAGC	1 cut(s)	2438
AvaI	CYCGRG	2 cut(s)	270, 1330
AvaII	GGWCC	4 cut(s)	663, 1100, 1354, 1706
BaeGI	GKGCMC	2 cut(s)	1630, 1986
BanII	GRGCYC	1 cut(s)	1986
BbsI	GAAGAC	2 cut(s)	1113, 1725
Bbv12I	GWGCWC	2 cut(s)	1836, 2332
BccI	CCATC	7 cut(s)	664, 853, 953, 1111, 1546, 2216, 2242
BceAI	ACGGC	1 cut(s)	116
BcgI	CGANNNNNNTGC	2 cut(s)	443, 477
BciT130I	CCWGG	3 cut(s)	947, 1770, 2091
BcnI	CCSGG	5 cut(s)	1008, 1233, 1358, 1524, 1852
BcoDI	GTCTC	3 cut(s)	2034, 2361, 2381
BfaI	CTAG	5 cut(s)	1400, 1701, 1730, 2265, 2439
BfmI	CTRYAG	6 cut(s)	798, 1174, 1375, 1663, 2073, 2311
BfoI	RGCGCY	1 cut(s)	2084
BfrI	CTTAAG	1 cut(s)	2398
BlpI	GCTNAGC	1 cut(s)	1995
BmcAI	AGTACT	1 cut(s)	1669
Bme1390I	CCNGG	8 cut(s)	947, 1008, 1233, 1358, 1524, 1770, 1852, 2091
Bme18I	GGWCC	4 cut(s)	663, 1100, 1354, 1706
BmeT110I	CYCGRG	2 cut(s)	270, 1330
BmgT120I	GGNCC	6 cut(s)	663, 1100, 1354, 1706, 1982, 1983
BmiI	GGNNCC	6 cut(s)	664, 864, 1101, 1364, 1984, 2157
BmrFI	CCNGG	8 cut(s)	947, 1008, 1233, 1358, 1524, 1770, 1852, 2091
BmtI	GCTAGC	1 cut(s)	2442
BoxI	GACNNNNGTC	1 cut(s)	2393
BpiI	GAAGAC	2 cut(s)	1113, 1725
BpmI	CTGGAG	4 cut(s)	540, 1236, 2016, 2344
Bpu10I	CCTNAGC	1 cut(s)	954
Bpu1102I	GCTNAGC	1 cut(s)	1995
BpuMI	CCSGG	5 cut(s)	1008, 1233, 1358, 1524, 1852
BsaBI	GATNNNNATC	1 cut(s)	511
BsaHI	GRCGYC	1 cut(s)	1000
BsaJI	CCNNGG	9 cut(s)	218, 461, 808, 1133, 1357, 1470, 1554, 1584, 2090
BsaWI	WCCGGW	3 cut(s)	646, 1714, 2177
BsaXI	ACNNNNNCTCC	8 cut(s)	442, 472, 477, 507, 513, 543, 1660, 1690
Bsc4I	CCNNNNNNNGG	4 cut(s)	379, 672, 761, 1623
Bse118I	RCCGGY	2 cut(s)	996, 2177
Bse1I	ACTGG	8 cut(s)	556, 608, 1219, 1899, 2026, 2109, 2260, 2379
Bse3DI	GCAATG	3 cut(s)	792, 1848, 1932
Bse8I	GATNNNNATC	1 cut(s)	511
BseAI	TCCGGA	2 cut(s)	646, 1714
BseBI	CCWGG	3 cut(s)	947, 1770, 2091
BseDI	CCNNGG	9 cut(s)	218, 461, 808, 1133, 1357, 1470, 1554, 1584, 2090
BseGI	GGATG	5 cut(s)	54, 864, 964, 1480, 1538
BseJI	GATNNNNATC	1 cut(s)	511
BseLI	CCNNNNNNNGG	4 cut(s)	379, 672, 761, 1623
BseMI	GCAATG	3 cut(s)	792, 1848, 1932
BseMII	CTCAG	6 cut(s)	130, 186, 752, 968, 2009, 2267
BseNI	ACTGG	8 cut(s)	556, 608, 1219, 1899, 2026, 2109, 2260, 2379
BseRI	GAGGAG	7 cut(s)	307, 412, 415, 450, 516, 855, 1860
BseSI	GKGCMC	2 cut(s)	1630, 1986
BseX3I	CGGCCG	1 cut(s)	129
BseYI	CCCAGC	2 cut(s)	830, 1880
BsgI	GTGCAG	2 cut(s)	1875, 1926
Bsh1285I	CGRYCG	1 cut(s)	132
BshFI	GGCC	2 cut(s)	131, 1984
BshTI	ACCGGT	1 cut(s)	2177
BsiEI	CGRYCG	1 cut(s)	132
BsiHKAI	GWGCWC	2 cut(s)	1836, 2332
BsiHKCI	CYCGRG	2 cut(s)	270, 1330
BslFI	GGGAC	2 cut(s)	1113, 1867
BslI	CCNNNNNNNGG	4 cut(s)	379, 672, 761, 1623
BsmAI	GTCTC	3 cut(s)	2034, 2361, 2381
BsmFI	GGGAC	2 cut(s)	1113, 1867
BsmI	GAATGC	1 cut(s)	1325
BsnI	GGCC	2 cut(s)	131, 1984
BsoBI	CYCGRG	2 cut(s)	270, 1330
Bsp120I	GGGCCC	1 cut(s)	1982
Bsp1286I	GDGCHC	4 cut(s)	1630, 1836, 1986, 2332
Bsp13I	TCCGGA	2 cut(s)	646, 1714
Bsp1407I	TGTACA	3 cut(s)	327, 897, 1248
Bsp1720I	GCTNAGC	1 cut(s)	1995
Bsp19I	CCATGG	3 cut(s)	808, 1133, 1470
BspACI	CCGC	3 cut(s)	186, 786, 2217
BspANI	GGCC	2 cut(s)	131, 1984
BspCNI	CTCAG	6 cut(s)	129, 187, 751, 967, 2008, 2268
BspEI	TCCGGA	2 cut(s)	646, 1714
BspHI	TCATGA	3 cut(s)	277, 1162, 1599
BspLI	GGNNCC	6 cut(s)	664, 864, 1101, 1364, 1984, 2157
BspMAI	CTGCAG	2 cut(s)	802, 1379
BspOI	GCTAGC	1 cut(s)	2442
BspPI	GGATC	5 cut(s)	554, 1412, 1706, 2158, 2459
BspQI	GCTCTTC	1 cut(s)	1159
BspTI	CTTAAG	1 cut(s)	2398
BsrDI	GCAATG	3 cut(s)	792, 1848, 1932
BsrFI	RCCGGY	2 cut(s)	996, 2177
BsrGI	TGTACA	3 cut(s)	327, 897, 1248
BsrI	ACTGG	8 cut(s)	556, 608, 1219, 1899, 2026, 2109, 2260, 2379
BssAI	RCCGGY	2 cut(s)	996, 2177
BssECI	CCNNGG	9 cut(s)	218, 461, 808, 1133, 1357, 1470, 1554, 1584, 2090
BssNI	GRCGYC	1 cut(s)	1000
BssT1I	CCWWGG	6 cut(s)	218, 808, 1133, 1470, 1554, 1584
Bst2UI	CCWGG	3 cut(s)	947, 1770, 2091
Bst4CI	ACNGT	7 cut(s)	572, 1147, 1294, 1667, 2272, 2284, 2537
Bst6I	CTCTTC	1 cut(s)	1159
BstACI	GRCGYC	1 cut(s)	1000
BstAFI	CTTAAG	1 cut(s)	2398
BstAPI	GCANNNNNTGC	1 cut(s)	707
BstAUI	TGTACA	3 cut(s)	327, 897, 1248
BstC8I	GCNNGC	5 cut(s)	691, 779, 1887, 2001, 2440
BstDEI	CTNAG	9 cut(s)	116, 195, 738, 954, 1426, 1445, 1995, 2276, 2471
BstDSI	CCRYGG	3 cut(s)	808, 1133, 1470
BstEII	GGTNACC	1 cut(s)	2219
BstENI	CCTNNNNNAGG	1 cut(s)	670
BstF5I	GGATG	5 cut(s)	54, 864, 964, 1480, 1538
BstH2I	RGCGCY	1 cut(s)	2084
BstHHI	GCGC	1 cut(s)	2083
BstMAI	GTCTC	3 cut(s)	2034, 2361, 2381
BstMCI	CGRYCG	1 cut(s)	132
BstMWI	GCNNNNNNNGC	8 cut(s)	707, 794, 912, 1061, 1271, 1886, 2000, 2078
BstNI	CCWGG	3 cut(s)	947, 1770, 2091
BstNSI	RCATGY	2 cut(s)	1120, 1274
BstPAI	GACNNNNGTC	1 cut(s)	2393
BstPI	GGTNACC	1 cut(s)	2219
BstSCI	CCNGG	8 cut(s)	945, 1006, 1231, 1356, 1522, 1768, 1850, 2089
BstSFI	CTRYAG	6 cut(s)	798, 1174, 1375, 1663, 2073, 2311
BstSLI	GKGCMC	2 cut(s)	1630, 1986
BstV2I	GAAGAC	2 cut(s)	1113, 1725
BstX2I	RGATCY	2 cut(s)	1404, 1711
BstXI	CCANNNNNNTGG	2 cut(s)	626, 1039
BstYI	RGATCY	2 cut(s)	1404, 1711
BstZI	CGGCCG	1 cut(s)	129
BsuRI	GGCC	2 cut(s)	131, 1984
BtgI	CCRYGG	3 cut(s)	808, 1133, 1470
BtgZI	GCGATG	1 cut(s)	862
BtsCI	GGATG	5 cut(s)	54, 864, 964, 1480, 1538
BtsI	GCAGTG	1 cut(s)	2062
BtsIMutI	CAGTG	4 cut(s)	1892, 2062, 2289, 2386
Cac8I	GCNNGC	5 cut(s)	691, 779, 1887, 2001, 2440
CciI	TCATGA	3 cut(s)	277, 1162, 1599
CfoI	GCGC	1 cut(s)	2083
Cfr10I	RCCGGY	2 cut(s)	996, 2177
Cfr13I	GGNCC	6 cut(s)	663, 1100, 1354, 1706, 1982, 1983
CseI	GACGC	1 cut(s)	989
Csp6I	GTAC	4 cut(s)	328, 898, 1249, 1668
CspAI	ACCGGT	1 cut(s)	2177
CviQI	GTAC	4 cut(s)	328, 898, 1249, 1668
DdeI	CTNAG	9 cut(s)	116, 195, 738, 954, 1426, 1445, 1995, 2276, 2471
DrdI	GACNNNNNNGTC	1 cut(s)	2472
DseDI	GACNNNNNNGTC	1 cut(s)	2472
EaeI	YGGCCR	1 cut(s)	129
EagI	CGGCCG	1 cut(s)	129
Eam1104I	CTCTTC	1 cut(s)	1159
EarI	CTCTTC	1 cut(s)	1159
EclXI	CGGCCG	1 cut(s)	129
Eco130I	CCWWGG	6 cut(s)	218, 808, 1133, 1470, 1554, 1584
Eco24I	GRGCYC	1 cut(s)	1986
Eco32I	GATATC	1 cut(s)	1597
Eco47I	GGWCC	4 cut(s)	663, 1100, 1354, 1706
Eco47III	AGCGCT	1 cut(s)	2082
Eco52I	CGGCCG	1 cut(s)	129
Eco57I	CTGAAG	2 cut(s)	341, 1111
Eco88I	CYCGRG	2 cut(s)	270, 1330
Eco91I	GGTNACC	1 cut(s)	2219
EcoNI	CCTNNNNNAGG	1 cut(s)	670
EcoO109I	RGGNCCY	1 cut(s)	663
EcoO65I	GGTNACC	1 cut(s)	2219
EcoRI	GAATTC	1 cut(s)	813
EcoRII	CCWGG	3 cut(s)	945, 1768, 2089
EcoRV	GATATC	1 cut(s)	1597
EcoT14I	CCWWGG	6 cut(s)	218, 808, 1133, 1470, 1554, 1584
EcoT22I	ATGCAT	1 cut(s)	2011
EcoT38I	GRGCYC	1 cut(s)	1986
ErhI	CCWWGG	6 cut(s)	218, 808, 1133, 1470, 1554, 1584
FaqI	GGGAC	2 cut(s)	1113, 1867
FokI	GGATG	5 cut(s)	61, 871, 971, 1487, 1525
FriOI	GRGCYC	1 cut(s)	1986
FspBI	CTAG	5 cut(s)	1400, 1701, 1730, 2265, 2439
GlaI	GCGC	1 cut(s)	2082
GsaI	CCCAGC	2 cut(s)	834, 1884
GsuI	CTGGAG	4 cut(s)	540, 1236, 2016, 2344
HaeII	RGCGCY	1 cut(s)	2084
HaeIII	GGCC	2 cut(s)	131, 1984
HgaI	GACGC	1 cut(s)	989
HhaI	GCGC	1 cut(s)	2083
Hin1I	GRCGYC	1 cut(s)	1000
Hin6I	GCGC	1 cut(s)	2081
HinP1I	GCGC	1 cut(s)	2081
HincII	GTYRAC	2 cut(s)	967, 2485
HindII	GTYRAC	2 cut(s)	967, 2485
HindIII	AAGCTT	5 cut(s)	7, 389, 677, 904, 976
HinfI	GANTC	7 cut(s)	80, 281, 512, 1328, 1550, 1811, 2086
HphI	GGTGA	8 cut(s)	1098, 1135, 1198, 1328, 1459, 1841, 2192, 2231
Hpy166II	GTNNAC	9 cut(s)	103, 136, 476, 872, 886, 967, 1251, 1467, 2485
Hpy8I	GTNNAC	9 cut(s)	103, 136, 476, 872, 886, 967, 1251, 1467, 2485
HpyAV	CCTTC	6 cut(s)	150, 518, 761, 1135, 1935, 2344
HpyCH4III	ACNGT	7 cut(s)	572, 1147, 1294, 1667, 2272, 2284, 2537
HpyCH4IV	ACGT	3 cut(s)	598, 2195, 2292
HpyF10VI	GCNNNNNNNGC	8 cut(s)	707, 794, 912, 1061, 1271, 1886, 2000, 2078
HpyF3I	CTNAG	9 cut(s)	116, 195, 738, 954, 1426, 1445, 1995, 2276, 2471
HpySE526I	ACGT	3 cut(s)	598, 2195, 2292
Hsp92I	GRCGYC	1 cut(s)	1000
HspAI	GCGC	1 cut(s)	2081
Kpn2I	TCCGGA	2 cut(s)	646, 1714
LguI	GCTCTTC	1 cut(s)	1159
LmnI	GCTCC	9 cut(s)	130, 159, 485, 868, 1681, 1771, 1873, 2161, 2335
MaeI	CTAG	5 cut(s)	1400, 1701, 1730, 2265, 2439
MaeII	ACGT	3 cut(s)	598, 2195, 2292
MaeIII	GTNAC	6 cut(s)	109, 367, 640, 1204, 2219, 2423
MboII	GAAGA	4 cut(s)	89, 1113, 1146, 1730
MfeI	CAATTG	1 cut(s)	1452
MflI	RGATCY	2 cut(s)	1404, 1711
MhlI	GDGCHC	4 cut(s)	1630, 1836, 1986, 2332
MlyI	GAGTC	2 cut(s)	1322, 1805
MmeI	TCCRAC	3 cut(s)	587, 1201, 2171
Mph1103I	ATGCAT	1 cut(s)	2011
MroI	TCCGGA	2 cut(s)	646, 1714
MroXI	GAANNNNTTC	1 cut(s)	232
MseI	TTAA	5 cut(s)	1341, 1500, 2399, 2412, 2539
MslI	CAYNNNNRTG	4 cut(s)	141, 186, 1037, 1292
MspA1I	CMGCKG	2 cut(s)	518, 1055
MspCI	CTTAAG	1 cut(s)	2398
MspR9I	CCNGG	8 cut(s)	947, 1008, 1233, 1358, 1524, 1770, 1852, 2091
MunI	CAATTG	1 cut(s)	1452
Mva1269I	GAATGC	1 cut(s)	1325
MvaI	CCWGG	3 cut(s)	947, 1770, 2091
MwoI	GCNNNNNNNGC	8 cut(s)	707, 794, 912, 1061, 1271, 1886, 2000, 2078
NciI	CCSGG	5 cut(s)	1008, 1233, 1358, 1524, 1852
NcoI	CCATGG	3 cut(s)	808, 1133, 1470
NheI	GCTAGC	1 cut(s)	2438
NlaIV	GGNNCC	6 cut(s)	664, 864, 1101, 1364, 1984, 2157
NmeAIII	GCCGAG	2 cut(s)	74, 172
NmuCI	GTSAC	3 cut(s)	109, 1204, 2219
NsiI	ATGCAT	1 cut(s)	2011
NspI	RCATGY	2 cut(s)	1120, 1274
OliI	CACNNNNGTG	1 cut(s)	1292
PaeR7I	CTCGAG	1 cut(s)	1330
PagI	TCATGA	3 cut(s)	277, 1162, 1599
PciSI	GCTCTTC	1 cut(s)	1159
PctI	GAATGC	1 cut(s)	1325
PdmI	GAANNNNTTC	1 cut(s)	232
PfeI	GAWTC	5 cut(s)	80, 281, 512, 1550, 2086
PfoI	TCCNGGA	3 cut(s)	1006, 1768, 1850
PinAI	ACCGGT	1 cut(s)	2177
PleI	GAGTC	2 cut(s)	1322, 1805
PpsI	GAGTC	2 cut(s)	1322, 1805
PpuMI	RGGWCCY	1 cut(s)	663
PshAI	GACNNNNGTC	1 cut(s)	2393
Psp1406I	AACGTT	1 cut(s)	2292
Psp5II	RGGWCCY	1 cut(s)	663
Psp6I	CCWGG	3 cut(s)	945, 1768, 2089
PspEI	GGTNACC	1 cut(s)	2219
PspFI	CCCAGC	2 cut(s)	830, 1880
PspGI	CCWGG	3 cut(s)	945, 1768, 2089
PspN4I	GGNNCC	6 cut(s)	664, 864, 1101, 1364, 1984, 2157
PspOMI	GGGCCC	1 cut(s)	1982
PspPI	GGNCC	6 cut(s)	663, 1100, 1354, 1706, 1982, 1983
PspPPI	RGGWCCY	1 cut(s)	663
PspXI	VCTCGAGB	1 cut(s)	1330
PstI	CTGCAG	2 cut(s)	802, 1379
PsuI	RGATCY	2 cut(s)	1404, 1711
PvuII	CAGCTG	2 cut(s)	518, 1055
RsaI	GTAC	4 cut(s)	329, 899, 1250, 1669
RsaNI	GTAC	4 cut(s)	328, 898, 1249, 1668
RseI	CAYNNNNRTG	4 cut(s)	141, 186, 1037, 1292
SapI	GCTCTTC	1 cut(s)	1159
SaqAI	TTAA	5 cut(s)	1341, 1500, 2399, 2412, 2539
Sau96I	GGNCC	6 cut(s)	663, 1100, 1354, 1706, 1982, 1983
ScaI	AGTACT	1 cut(s)	1669
SchI	GAGTC	2 cut(s)	1322, 1805
ScrFI	CCNGG	8 cut(s)	947, 1008, 1233, 1358, 1524, 1770, 1852, 2091
SduI	GDGCHC	4 cut(s)	1630, 1836, 1986, 2332
SfcI	CTRYAG	6 cut(s)	798, 1174, 1375, 1663, 2073, 2311
Sfr274I	CTCGAG	1 cut(s)	1330
SgrAI	CRCCGGYG	1 cut(s)	2177
SinI	GGWCC	4 cut(s)	663, 1100, 1354, 1706
SlaI	CTCGAG	1 cut(s)	1330
SmiMI	CAYNNNNRTG	4 cut(s)	141, 186, 1037, 1292
SmlI	CTYRAG	2 cut(s)	1330, 2398
SmoI	CTYRAG	2 cut(s)	1330, 2398
SsiI	CCGC	3 cut(s)	186, 786, 2217
SspI	AATATT	1 cut(s)	232
SspMI	CTAG	5 cut(s)	1400, 1701, 1730, 2265, 2439
StyD4I	CCNGG	8 cut(s)	945, 1006, 1231, 1356, 1522, 1768, 1850, 2089
StyI	CCWWGG	6 cut(s)	218, 808, 1133, 1470, 1554, 1584
TaaI	ACNGT	7 cut(s)	572, 1147, 1294, 1667, 2272, 2284, 2537
TaiI	ACGT	3 cut(s)	601, 2198, 2295
TaqI	TCGA	6 cut(s)	236, 395, 817, 1331, 1814, 2372
TatI	WGTACW	4 cut(s)	327, 897, 1248, 1667
TfiI	GAWTC	5 cut(s)	80, 281, 512, 1550, 2086
Tru1I	TTAA	5 cut(s)	1341, 1500, 2399, 2412, 2539
Tru9I	TTAA	5 cut(s)	1341, 1500, 2399, 2412, 2539
TscAI	CASTG	4 cut(s)	1899, 2062, 2289, 2386
TseFI	GTSAC	3 cut(s)	109, 1204, 2219
Tsp45I	GTSAC	3 cut(s)	109, 1204, 2219
TspDTI	ATGAA	9 cut(s)	266, 273, 525, 723, 1529, 1763, 2319, 2334, 2354
TspGWI	ACGGA	1 cut(s)	777
TspRI	CASTG	4 cut(s)	1899, 2062, 2289, 2386
Vha464I	CTTAAG	1 cut(s)	2398
VpaK11BI	GGWCC	4 cut(s)	663, 1100, 1354, 1706
XagI	CCTNNNNNAGG	1 cut(s)	670
XapI	RAATTY	4 cut(s)	813, 1619, 1774, 1820
XbaI	TCTAGA	2 cut(s)	1729, 2264
XceI	RCATGY	2 cut(s)	1120, 1274
XcmI	CCANNNNNNNNNTGG	1 cut(s)	486
XhoI	CTCGAG	1 cut(s)	1330
XmnI	GAANNNNTTC	1 cut(s)	232
XspI	CTAG	5 cut(s)	1400, 1701, 1730, 2265, 2439
ZrmI	AGTACT	1 cut(s)	1669
Zsp2I	ATGCAT	1 cut(s)	2011

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.