Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0007288

Physical Location & Seq

Genomic Coordinates Forward (+)

11450552 .. 11456007

Gene Structure 5456 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT4G21530.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2334 bp

ATGTCGGAAATGGCGAGTGACGAGGAAGAAAATATCATTCCTTTCCAGCTCCAGTTCGACAAGCCCATACCTTTTCAGATTAAGATAGCTGAGTGGAACCCGGAGAAGGATTTACTGGCAATGGTTACTGAGGACTCCAAGATTTTGCTGCATCGGTTCAATTGGCAGAGATTATGGACTATTTCGCCTGGAAAGCCTGTTACTTCTTTATGTTGGCGTCCTGATGGCAAAGCCATAGCTGTTGGGCTCGAGGATGGAACCATTTCACTTCACGATGTTGAGAATGGGAAGCTATTAAGGAACTTGAAGCCCCATGATGTAGCTGTCGTGTGTCTTAACTGGGAGGAGGATGGACAATCCAATACTGATGAAAGTGGTAATTTCTCAGTTTATGAGGACCGGACCTCCCGTTTTTTTCCTCCTGCTCCAAGACCTCCAAAGATGCCTGGACTGGTAGCTGGTGACTCCAGTTTCATGGATGACGGTGAAGACTCTTTGGCAGAGCTATCCAATACCTCATTCCGGAAGTTTAACATTCTTTGCACTGGTGATCGTGATGGGAACATATGCTTCAGTATATTTGGAATATTTCAGATAGGAAAGATTAACATACACGAGCTGTCTCTTCCTGTGCCTCATCTCGATGAGCATGCCTCTTGCAAACTATTTAATGCTTCCATTTACAAGGTTGCTCTGTCAAAAGACCTTTGCCGTCTGGTAGTAATGTGCACAGGAGAGCTTAAGGATTGTGATATCAAGCCAAGAGAGGAAAAAATAAATGTTCAAGATCTGCCTGGTTTACATTGCCTTGCAATGGACACATCTATATTTTGGAAAAGGAAATATGAGCTTCATCAGGTTGCTCAACAAGCTTCTAACATTGAAGATTTGACCGAGGTTATTCGAGCATCCCTGTCAGTTATGAACAAACAATGGGCGGATGCAATGAAAACTTTTCATGAAAAGTTTCATTCTCTTTCCACCTTGATCATTGATAATGGACTGGAATCATCTCCTCAAGAAGAATTTCTTAGCCTTTTGGGTGGTGCACGAATAAGCCCAGCGCTTAATCAGTTTTTAGTTAACTCTCTTGGCGAAGTGGGTGTGAAGCGTGTTTTAAAGTCAGTATGTGGTACAGGAAAAGAGCTTCAGCAGGTTGTCCTTGACCATCTTCAGCCTGCAGCAGAAATTATCGGATTCAGGATAGGTGAATTAAGAGGTCTATCGAGGTGGCGTGCACGGTACCAAGGCATTGGTTTGGATGAGATGCTCCTTAATGAAGCCACTGAAAATACTGGTCTGCTACTTGTACAAGTGCAACGGTTTATGATGGTACTATCTTCTGTTGTCCAACAGTTCTCAAATTTCTTCAACTGGCTCGTCAGAAGTATAAAGTATCTTATGCAAGAACCGAATGATCAGCTTTTGTCATACAACAGTGAACTCCTTGTGGTATTCTTGAAGTTCTTATATGACCAAGATCCTGTGAAAGACCTCTTGGAGCTATCTGAAGCTGGTGATGACATTGAAATTGATCTCAAAACCATCGGAAGAGTCAAAGAGTTACTTCAGTTTGGAGGGTTTTCAGAGTGTGATTTTCTGCAGAGGACATTGGCTAAGGAATTCCAACACATGGAGTCTAGCTTCAAGATGGCTTTTCAAATGCCCTTTACAACAATATCAAGAAAGATATCATGTATGAAGTTGTTGCCTCTTTGCCCTCTTCAGTTGTCAACAACGCAAACACCTACAACAATTCCCATGTCTCTTTCCTTCTATAAGAATGAACTTTCTGATGATACACCTTGTCAAAGTGGGTACACTGATTACATATCTTTTCAAGTCCCCGATGAGACCTTCCCAGAGATTTCAAACTGTATTGGCATAGCTAAGGGTTATAAGCAGAATTCCAACAATGAGAAAAATGGTTATACTTCTCTGGAAGCCGTTTTGTTATCTGTACCTAATGGCTACACTTGTGTTGATTTGTCTCTCTACAAGGATAAAGAACTGGTTTTATTATTGAATAAGACAAATACAGATTCCGAAGGCTCGGGAGAAGCATGCATGATGGTTGTACAAACTGGTGACCTCGCATTCATTTCCATTTCAGGATCTAGTTCTCTAAACCAGTGGGAGCTAGAGGATTTGAAGGGCTCGATTGTGAACCTGGAAATGGAGAATGAAAAGGTTCGCAAAGTACCACATTCTGTCATTGCTCCTCTAGCAGTAAGCGCTTCAAGAGGAGTGGCTTGTGTTTTTGCTGAAAGAAGACGAGCTTTGGTGTACATATTGGAAGAAGATGAAGATGAAGAAATCTCTGATGAAAAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0007275 GO:0008150 GO:0009790 GO:0009791 GO:0009793 GO:0009908 GO:0010154 GO:0022414 GO:0032501 GO:0032502 GO:0035670 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0048316 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048467 GO:0048481 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0061458 GO:0090567 GO:0099402

KEGG Pathways

Metabolic & Signaling

ko04110 ko04111 ko04113 ko04114 ko04120 ko04914 ko05166 map04110 map04111 map04113 map04114 map04120 map04914 map05166

Pfam Domains

Protein Families

ANAPC4 ANAPC4_WD40

Protein Analysis

777

Amino Acids

87.42

Weight (kDa)

4.86

Isoelectric Point (pI)

44.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_WDR19_1st	PF23389	26 - 118	8.2e-07	WDR19 first beta-propeller
WD40_CDC20-Fz	PF24807	27 - 137	1e-11	CDC20/Fizzy WD40 domain
Beta-prop_EML_2	PF23414	28 - 120	8.5e-08	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR35_TULP_N	PF24797	28 - 120	2.8e-06	WDR35/TULP4 N-terminal
Beta-prop_THOC3	PF25174	30 - 120	2.3e-09	THOC3 beta-propeller domain
WD40_WDHD1_1st	PF24817	30 - 128	4.5e-09	WDHD1 first WD40 domain
Beta-prop_WDR5	PF25175	30 - 118	9.6e-08	WDR5 beta-propeller domain
ANAPC4_WD40	PF12894	31 - 116	1e-29	Anaphase-promoting complex subunit 4 WD40 domain
WDR55	PF24796	31 - 136	2.3e-06	WDR55
WD40_Prp19	PF24814	32 - 118	2.5e-06	Prp19 WD40 domain
Beta-prop_WDR3_1st	PF25173	65 - 119	1.8e-08	WDR3 first beta-propeller domain
ANAPC4	PF12896	268 - 470	9.8e-57	Anaphase-promoting complex, cyclosome, subunit 4

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0007288)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G21530
fragaria_vesca	FvH4_3g04050 FvH4_3g04050 FvH4_3g04050
malus_domestica	MD10G1303000.v1.1
prunus_persica	Prupe.4G036500_v2.0.a1 Prupe.4G036500_v2.0.a1 Prupe.4G036500_v2.0.a1 Prupe.4G036500_v2.0.a1 Prupe.4G036500_v2.0.a1 Prupe.4G036500_v2.0.a1
pyrus_communis	pycom10g25560
rosa_chinensis	RchiOBHm_Chr5g0006531
rosa_laevigata	RLG00000031396 RLG00000034652
rosa_multiflora	Rmu_sc0008991.1_g000016
rosa_roxburghii	Rroxscaffold_1G00031790 Rroxscaffold_1G00069530
rosa_rugosa	Rorug04G0421700
rosa_samantha	Rh5AG049400 Rh5BG048700 Rh5CG057000 Rh5DG048000
rosa_wichuraiana	Rw5G004950 Rw5G030980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1899
Acc36I	ACCTGC	1 cut(s)	1144
Acc65I	GGTACC	1 cut(s)	1242
AccB1I	GGYRCC	1 cut(s)	1242
AccB7I	CCANNNNNTGG	1 cut(s)	1633
AccIII	TCCGGA	1 cut(s)	522
AciI	CCGC	1 cut(s)	938
AclWI	GGATC	2 cut(s)	1475, 2122
AcsI	RAATTY	4 cut(s)	1025, 1363, 1622, 1906
AcuI	CTGAAG	6 cut(s)	556, 1133, 1157, 1530, 1553, 1709
AcyI	GRCGYC	1 cut(s)	217
AfaI	GTAC	9 cut(s)	1135, 1244, 1311, 1335, 1820, 1962, 2079, 2202, 2288
AfeI	AGCGCT	2 cut(s)	1065, 2236
AfiI	CCNNNNNNNGG	5 cut(s)	106, 522, 814, 1633, 2176
AflII	CTTAAG	1 cut(s)	740
AjnI	CCWGG	4 cut(s)	187, 445, 793, 2169
AjuI	GAANNNNNNNTTGG	4 cut(s)	503, 535, 1481, 1513
Alw21I	GWGCWC	3 cut(s)	731, 1051, 1240
Alw26I	GTCTC	4 cut(s)	627, 1770, 1847, 1995
Alw44I	GTGCAC	3 cut(s)	727, 1047, 1236
AlwI	GGATC	2 cut(s)	1475, 2122
Ama87I	CYCGRG	2 cut(s)	248, 2053
Aor13HI	TCCGGA	1 cut(s)	522
Aor51HI	AGCGCT	2 cut(s)	1065, 2236
ApaLI	GTGCAC	3 cut(s)	727, 1047, 1236
ApeKI	GCWGC	2 cut(s)	148, 1181
ApoI	RAATTY	4 cut(s)	1025, 1363, 1622, 1906
Asp700I	GAANNNNTTC	4 cut(s)	262, 966, 1026, 2190
Asp718I	GGTACC	1 cut(s)	1242
AspLEI	GCGC	2 cut(s)	1066, 2237
AspS9I	GGNCC	2 cut(s)	397, 402
AsuC2I	CCSGG	1 cut(s)	101
AsuHPI	GGTGA	6 cut(s)	473, 497, 560, 1220, 1529, 2099
AvaI	CYCGRG	2 cut(s)	248, 2053
AvaII	GGWCC	2 cut(s)	397, 402
BaeGI	GKGCMC	3 cut(s)	731, 1051, 1240
BanI	GGYRCC	1 cut(s)	1242
BanII	GRGCYC	2 cut(s)	249, 2159
BauI	CACGAG	1 cut(s)	614
BbsI	GAAGAC	2 cut(s)	495, 2278
Bbv12I	GWGCWC	3 cut(s)	731, 1051, 1240
BbvI	GCAGC	2 cut(s)	135, 1193
BccI	CCATC	9 cut(s)	218, 248, 344, 551, 1176, 1324, 1553, 1645, 2065
BceAI	ACGGC	2 cut(s)	696, 1931
BcgI	CGANNNNNNTGC	2 cut(s)	632, 666
BciT130I	CCWGG	4 cut(s)	189, 447, 795, 2171
BclI	TGATCA	2 cut(s)	987, 1417
BcnI	CCSGG	1 cut(s)	101
BcoDI	GTCTC	4 cut(s)	627, 1770, 1847, 1995
BfaI	CTAG	4 cut(s)	1641, 2118, 2141, 2225
BfmI	CTRYAG	2 cut(s)	1179, 1601
BfoI	RGCGCY	2 cut(s)	1067, 2238
BfrI	CTTAAG	1 cut(s)	740
BfuAI	ACCTGC	1 cut(s)	1144
BglII	AGATCT	1 cut(s)	787
BisI	GCNGC	2 cut(s)	149, 1182
BlsI	GCNGC	2 cut(s)	150, 1183
Bme1390I	CCNGG	5 cut(s)	101, 189, 447, 795, 2171
Bme18I	GGWCC	2 cut(s)	397, 402
BmeT110I	CYCGRG	2 cut(s)	248, 2053
BmgT120I	GGNCC	2 cut(s)	397, 402
BmiI	GGNNCC	3 cut(s)	98, 259, 1244
BmrFI	CCNGG	5 cut(s)	101, 189, 447, 795, 2171
BmrI	ACTGGG	1 cut(s)	349
BmsI	GCATC	5 cut(s)	160, 432, 917, 931, 1257
BmuI	ACTGGG	1 cut(s)	349
BpiI	GAAGAC	2 cut(s)	495, 2278
BplI	GAGNNNNNCTC	2 cut(s)	638, 670
BpmI	CTGGAG	2 cut(s)	35, 451
Bpu10I	CCTNAGC	2 cut(s)	1617, 1890
BpuEI	CTTGAG	1 cut(s)	1002
BpuMI	CCSGG	1 cut(s)	101
BsaHI	GRCGYC	1 cut(s)	217
BsaI	GGTCTC	1 cut(s)	1847
BsaJI	CCNNGG	2 cut(s)	894, 1246
BsaWI	WCCGGW	2 cut(s)	399, 522
BsaXI	ACNNNNNCTCC	2 cut(s)	389, 419
Bsc4I	CCNNNNNNNGG	5 cut(s)	106, 522, 814, 1633, 2176
Bse3DI	GCAATG	5 cut(s)	126, 802, 819, 951, 2214
BseAI	TCCGGA	1 cut(s)	522
BseBI	CCWGG	4 cut(s)	189, 447, 795, 2171
BseDI	CCNNGG	2 cut(s)	894, 1246
BseGI	GGATG	6 cut(s)	259, 355, 484, 908, 946, 1267
BseLI	CCNNNNNNNGG	5 cut(s)	106, 522, 814, 1633, 2176
BseMI	GCAATG	5 cut(s)	126, 802, 819, 951, 2214
BseMII	CTCAG	3 cut(s)	81, 120, 399
BseRI	GAGGAG	4 cut(s)	359, 1005, 2211, 2259
BseSI	GKGCMC	3 cut(s)	731, 1051, 1240
BseXI	GCAGC	2 cut(s)	135, 1193
BseYI	CCCAGC	1 cut(s)	1060
BshNI	GGYRCC	1 cut(s)	1242
BsiHKAI	GWGCWC	3 cut(s)	731, 1051, 1240
BsiHKCI	CYCGRG	2 cut(s)	248, 2053
BsiSI	CCGG	3 cut(s)	101, 400, 523
BslFI	GGGAC	1 cut(s)	1829
BslI	CCNNNNNNNGG	5 cut(s)	106, 522, 814, 1633, 2176
BsmAI	GTCTC	4 cut(s)	627, 1770, 1847, 1995
BsmFI	GGGAC	1 cut(s)	1829
BsmI	GAATGC	1 cut(s)	2096
Bso31I	GGTCTC	1 cut(s)	1847
BsoBI	CYCGRG	2 cut(s)	248, 2053
Bsp1286I	GDGCHC	5 cut(s)	249, 731, 1051, 1240, 2159
Bsp13I	TCCGGA	1 cut(s)	522
Bsp1407I	TGTACA	3 cut(s)	1309, 2077, 2286
Bsp143I	GATC	7 cut(s)	550, 787, 987, 1417, 1480, 1534, 2114
BspACI	CCGC	1 cut(s)	938
BspCNI	CTCAG	3 cut(s)	82, 121, 398
BspEI	TCCGGA	1 cut(s)	522
BspHI	TCATGA	1 cut(s)	958
BspLI	GGNNCC	3 cut(s)	98, 259, 1244
BspMAI	CTGCAG	2 cut(s)	1183, 1605
BspMI	ACCTGC	1 cut(s)	1144
BspPI	GGATC	2 cut(s)	1475, 2122
BspT107I	GGYRCC	1 cut(s)	1242
BspTI	CTTAAG	1 cut(s)	740
BspTNI	GGTCTC	1 cut(s)	1847
BsrDI	GCAATG	5 cut(s)	126, 802, 819, 951, 2214
BsrGI	TGTACA	3 cut(s)	1309, 2077, 2286
BssECI	CCNNGG	2 cut(s)	894, 1246
BssMI	GATC	7 cut(s)	550, 787, 987, 1417, 1480, 1534, 2114
BssNI	GRCGYC	1 cut(s)	217
BssSI	CACGAG	1 cut(s)	614
BssT1I	CCWWGG	1 cut(s)	1246
Bst2BI	CACGAG	1 cut(s)	614
Bst2UI	CCWGG	4 cut(s)	189, 447, 795, 2171
Bst4CI	ACNGT	6 cut(s)	485, 1242, 1323, 1356, 1439, 1877
Bst6I	CTCTTC	3 cut(s)	630, 1546, 1728
BstACI	GRCGYC	1 cut(s)	217
BstAFI	CTTAAG	1 cut(s)	740
BstAUI	TGTACA	3 cut(s)	1309, 2077, 2286
BstC8I	GCNNGC	4 cut(s)	651, 1179, 1236, 2065
BstDEI	CTNAG	6 cut(s)	90, 129, 385, 1031, 1617, 1890
BstEII	GGTNACC	1 cut(s)	2087
BstF5I	GGATG	6 cut(s)	259, 355, 484, 908, 946, 1267
BstH2I	RGCGCY	2 cut(s)	1067, 2238
BstHHI	GCGC	2 cut(s)	1066, 2237
BstKTI	GATC	7 cut(s)	553, 790, 990, 1420, 1483, 1537, 2117
BstMAI	GTCTC	4 cut(s)	627, 1770, 1847, 1995
BstMBI	GATC	7 cut(s)	550, 787, 987, 1417, 1480, 1534, 2114
BstMWI	GCNNNNNNNGC	4 cut(s)	193, 869, 2225, 2234
BstNI	CCWGG	4 cut(s)	189, 447, 795, 2171
BstNSI	RCATGY	2 cut(s)	653, 2067
BstPI	GGTNACC	1 cut(s)	2087
BstSCI	CCNGG	5 cut(s)	99, 187, 445, 793, 2169
BstSFI	CTRYAG	2 cut(s)	1179, 1601
BstSLI	GKGCMC	3 cut(s)	731, 1051, 1240
BstV1I	GCAGC	2 cut(s)	135, 1193
BstV2I	GAAGAC	2 cut(s)	495, 2278
BstX2I	RGATCY	3 cut(s)	787, 1480, 2114
BstXI	CCANNNNNNTGG	2 cut(s)	475, 1253
BstYI	RGATCY	3 cut(s)	787, 1480, 2114
BtsCI	GGATG	6 cut(s)	259, 355, 484, 908, 946, 1267
BtsIMutI	CAGTG	5 cut(s)	543, 1284, 1444, 1821, 2138
BveI	ACCTGC	1 cut(s)	1144
Cac8I	GCNNGC	4 cut(s)	651, 1179, 1236, 2065
CciI	TCATGA	1 cut(s)	958
CfoI	GCGC	2 cut(s)	1066, 2237
Cfr13I	GGNCC	2 cut(s)	397, 402
CseI	GACGC	1 cut(s)	206
Csp6I	GTAC	9 cut(s)	1134, 1243, 1310, 1334, 1819, 1961, 2078, 2201, 2287
CspCI	CAANNNNNGTGG	2 cut(s)	2229, 2264
CviAII	CATG	9 cut(s)	314, 475, 650, 959, 1633, 1695, 1762, 2064, 2068
CviQI	GTAC	9 cut(s)	1134, 1243, 1310, 1334, 1819, 1961, 2078, 2201, 2287
DdeI	CTNAG	6 cut(s)	90, 129, 385, 1031, 1617, 1890
DpnI	GATC	7 cut(s)	552, 789, 989, 1419, 1482, 1536, 2116
DpnII	GATC	7 cut(s)	550, 787, 987, 1417, 1480, 1534, 2114
DraI	TTTAAA	1 cut(s)	1119
Eam1104I	CTCTTC	3 cut(s)	630, 1546, 1728
EarI	CTCTTC	3 cut(s)	630, 1546, 1728
EciI	GGCGGA	1 cut(s)	953
Eco130I	CCWWGG	1 cut(s)	1246
Eco24I	GRGCYC	2 cut(s)	249, 2159
Eco31I	GGTCTC	1 cut(s)	1847
Eco32I	GATATC	2 cut(s)	754, 1692
Eco47I	GGWCC	2 cut(s)	397, 402
Eco47III	AGCGCT	2 cut(s)	1065, 2236
Eco57I	CTGAAG	6 cut(s)	556, 1133, 1157, 1530, 1553, 1709
Eco88I	CYCGRG	2 cut(s)	248, 2053
Eco91I	GGTNACC	1 cut(s)	2087
EcoO65I	GGTNACC	1 cut(s)	2087
EcoRI	GAATTC	2 cut(s)	1622, 1906
EcoRII	CCWGG	4 cut(s)	187, 445, 793, 2169
EcoRV	GATATC	2 cut(s)	754, 1692
EcoT14I	CCWWGG	1 cut(s)	1246
EcoT22I	ATGCAT	1 cut(s)	2069
EcoT38I	GRGCYC	2 cut(s)	249, 2159
ErhI	CCWWGG	1 cut(s)	1246
FaeI	CATG	9 cut(s)	317, 478, 653, 962, 1636, 1698, 1765, 2067, 2071
FalI	AAGNNNNNCTT	6 cut(s)	1014, 1046, 1551, 1583, 2263, 2295
FaqI	GGGAC	1 cut(s)	1829
FatI	CATG	9 cut(s)	313, 474, 649, 958, 1632, 1694, 1761, 2063, 2067
FauNDI	CATATG	1 cut(s)	566
FbaI	TGATCA	2 cut(s)	987, 1417
Fnu4HI	GCNGC	2 cut(s)	149, 1182
FokI	GGATG	6 cut(s)	266, 362, 491, 895, 953, 1274
FriOI	GRGCYC	2 cut(s)	249, 2159
Fsp4HI	GCNGC	2 cut(s)	149, 1182
FspBI	CTAG	4 cut(s)	1641, 2118, 2141, 2225
GlaI	GCGC	2 cut(s)	1065, 2236
GluI	GCNGC	2 cut(s)	149, 1182
GsaI	CCCAGC	1 cut(s)	1064
GsuI	CTGGAG	2 cut(s)	35, 451
HaeII	RGCGCY	2 cut(s)	1067, 2238
HapII	CCGG	3 cut(s)	101, 400, 523
HgaI	GACGC	1 cut(s)	206
HhaI	GCGC	2 cut(s)	1066, 2237
Hin1I	GRCGYC	1 cut(s)	217
Hin1II	CATG	9 cut(s)	317, 478, 653, 962, 1636, 1698, 1765, 2067, 2071
Hin6I	GCGC	2 cut(s)	1064, 2235
HinP1I	GCGC	2 cut(s)	1064, 2235
HincII	GTYRAC	2 cut(s)	1084, 1734
HindII	GTYRAC	2 cut(s)	1084, 1734
HindIII	AAGCTT	1 cut(s)	870
HinfI	GANTC	8 cut(s)	134, 464, 491, 1007, 1197, 1554, 1637, 2042
HpaI	GTTAAC	1 cut(s)	1084
HpaII	CCGG	3 cut(s)	101, 400, 523
HphI	GGTGA	6 cut(s)	473, 497, 560, 1220, 1529, 2099
HpyAV	CCTTC	5 cut(s)	100, 1783, 1867, 2042, 2146
HpyCH4III	ACNGT	6 cut(s)	485, 1242, 1323, 1356, 1439, 1877
HpyF10VI	GCNNNNNNNGC	4 cut(s)	193, 869, 2225, 2234
HpyF3I	CTNAG	6 cut(s)	90, 129, 385, 1031, 1617, 1890
Hsp92I	GRCGYC	1 cut(s)	217
Hsp92II	CATG	9 cut(s)	317, 478, 653, 962, 1636, 1698, 1765, 2067, 2071
HspAI	GCGC	2 cut(s)	1064, 2235
Kpn2I	TCCGGA	1 cut(s)	522
KpnI	GGTACC	1 cut(s)	1246
Ksp22I	TGATCA	2 cut(s)	987, 1417
KspAI	GTTAAC	1 cut(s)	1084
Kzo9I	GATC	7 cut(s)	550, 787, 987, 1417, 1480, 1534, 2114
LmnI	GCTCC	6 cut(s)	54, 430, 1275, 1501, 2137, 2224
Lsp1109I	GCAGC	2 cut(s)	135, 1193
LweI	GCATC	5 cut(s)	160, 432, 917, 931, 1257
MaeI	CTAG	4 cut(s)	1641, 2118, 2141, 2225
MaeIII	GTNAC	6 cut(s)	17, 124, 199, 461, 1563, 2087
MalI	GATC	7 cut(s)	552, 789, 989, 1419, 1482, 1536, 2116
MboI	GATC	7 cut(s)	550, 787, 987, 1417, 1480, 1534, 2114
MfeI	CAATTG	1 cut(s)	160
MflI	RGATCY	3 cut(s)	787, 1480, 2114
MhlI	GDGCHC	5 cut(s)	249, 731, 1051, 1240, 2159
MlyI	GAGTC	5 cut(s)	128, 458, 485, 1563, 1646
MmeI	TCCRAC	3 cut(s)	1375, 1651, 1935
Mph1103I	ATGCAT	1 cut(s)	2069
MroI	TCCGGA	1 cut(s)	522
MroXI	GAANNNNTTC	4 cut(s)	262, 966, 1026, 2190
MslI	CAYNNNNRTG	1 cut(s)	642
MspCI	CTTAAG	1 cut(s)	740
MspI	CCGG	3 cut(s)	101, 400, 523
MspR9I	CCNGG	5 cut(s)	101, 189, 447, 795, 2171
MunI	CAATTG	1 cut(s)	160
Mva1269I	GAATGC	1 cut(s)	2096
MvaI	CCWGG	4 cut(s)	189, 447, 795, 2171
MwoI	GCNNNNNNNGC	4 cut(s)	193, 869, 2225, 2234
NciI	CCSGG	1 cut(s)	101
NdeI	CATATG	1 cut(s)	566
NdeII	GATC	7 cut(s)	550, 787, 987, 1417, 1480, 1534, 2114
NlaIII	CATG	9 cut(s)	317, 478, 653, 962, 1636, 1698, 1765, 2067, 2071
NlaIV	GGNNCC	3 cut(s)	98, 259, 1244
NmuCI	GTSAC	3 cut(s)	17, 461, 2087
NsiI	ATGCAT	1 cut(s)	2069
NspI	RCATGY	2 cut(s)	653, 2067
PaeI	GCATGC	2 cut(s)	653, 2067
PaeR7I	CTCGAG	1 cut(s)	248
PagI	TCATGA	1 cut(s)	958
PcsI	WCGNNNNNNNCGW	1 cut(s)	11
PctI	GAATGC	1 cut(s)	2096
PdmI	GAANNNNTTC	4 cut(s)	262, 966, 1026, 2190
PfeI	GAWTC	3 cut(s)	1007, 1197, 2042
PflMI	CCANNNNNTGG	1 cut(s)	1633
PkrI	GCNGC	2 cut(s)	150, 1183
PleI	GAGTC	5 cut(s)	128, 458, 485, 1562, 1645
PpsI	GAGTC	5 cut(s)	128, 458, 485, 1562, 1645
PsiI	TTATAA	1 cut(s)	1899
Psp6I	CCWGG	4 cut(s)	187, 445, 793, 2169
PspEI	GGTNACC	1 cut(s)	2087
PspFI	CCCAGC	1 cut(s)	1060
PspGI	CCWGG	4 cut(s)	187, 445, 793, 2169
PspN4I	GGNNCC	3 cut(s)	98, 259, 1244
PspPI	GGNCC	2 cut(s)	397, 402
PspXI	VCTCGAGB	1 cut(s)	248
PstI	CTGCAG	2 cut(s)	1183, 1605
PsuI	RGATCY	3 cut(s)	787, 1480, 2114
RsaI	GTAC	9 cut(s)	1135, 1244, 1311, 1335, 1820, 1962, 2079, 2202, 2288
RsaNI	GTAC	9 cut(s)	1134, 1243, 1310, 1334, 1819, 1961, 2078, 2201, 2287
RseI	CAYNNNNRTG	1 cut(s)	642
SatI	GCNGC	2 cut(s)	149, 1182
Sau3AI	GATC	7 cut(s)	550, 787, 987, 1417, 1480, 1534, 2114
Sau96I	GGNCC	2 cut(s)	397, 402
SchI	GAGTC	5 cut(s)	128, 458, 485, 1563, 1646
ScrFI	CCNGG	5 cut(s)	101, 189, 447, 795, 2171
SduI	GDGCHC	5 cut(s)	249, 731, 1051, 1240, 2159
SfaNI	GCATC	5 cut(s)	160, 432, 917, 931, 1257
SfcI	CTRYAG	2 cut(s)	1179, 1601
Sfr274I	CTCGAG	1 cut(s)	248
SinI	GGWCC	2 cut(s)	397, 402
SlaI	CTCGAG	1 cut(s)	248
SmiMI	CAYNNNNRTG	1 cut(s)	642
SmlI	CTYRAG	3 cut(s)	248, 740, 1017
SmoI	CTYRAG	3 cut(s)	248, 740, 1017
SphI	GCATGC	2 cut(s)	653, 2067
SsiI	CCGC	1 cut(s)	938
SspI	AATATT	1 cut(s)	588
SspMI	CTAG	4 cut(s)	1641, 2118, 2141, 2225
StyD4I	CCNGG	5 cut(s)	99, 187, 445, 793, 2169
StyI	CCWWGG	1 cut(s)	1246
TaaI	ACNGT	6 cut(s)	485, 1242, 1323, 1356, 1439, 1877
TaqI	TCGA	6 cut(s)	57, 249, 642, 904, 1226, 2159
TaqII	GACCGA	1 cut(s)	908
TatI	WGTACW	3 cut(s)	1309, 2077, 2286
TfiI	GAWTC	3 cut(s)	1007, 1197, 2042
TscAI	CASTG	5 cut(s)	550, 1291, 1444, 1828, 2138
TseFI	GTSAC	3 cut(s)	17, 461, 2087
TseI	GCWGC	2 cut(s)	148, 1181
Tsp45I	GTSAC	3 cut(s)	17, 461, 2087
TspRI	CASTG	5 cut(s)	550, 1291, 1444, 1828, 2138
Van91I	CCANNNNNTGG	1 cut(s)	1633
Vha464I	CTTAAG	1 cut(s)	740
VneI	GTGCAC	3 cut(s)	727, 1047, 1236
VpaK11BI	GGWCC	2 cut(s)	397, 402
XapI	RAATTY	4 cut(s)	1025, 1363, 1622, 1906
XceI	RCATGY	2 cut(s)	653, 2067
XhoI	CTCGAG	1 cut(s)	248
XmnI	GAANNNNTTC	4 cut(s)	262, 966, 1026, 2190
XspI	CTAG	4 cut(s)	1641, 2118, 2141, 2225
Zsp2I	ATGCAT	1 cut(s)	2069

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.