Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0007288

Physical Location & Seq

Genomic Coordinates Forward (+)

4754602 .. 4760818

Gene Structure 6217 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000031396

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2274 bp

ATGGAAACCGACAAAGAAGAACAACGCGTCACTCCATTCCAGCTCCAGTTCGACAAGCCAGTCGCTTCGCAGGTTAAGATCGCAGAGTGGAATCCCGAGAAGGATTTACTCGCCATGGTAACTGAAGACTCCAAGATTTTACTGCACCGCTTCAATTGGCAGAGGCTCTGGACAATCTCCCCAGGGAAGAGCATAACGTCTTTGTGTTGGAGACCTGATGGTAAAGCCATCGCTGTCGGGCTGGAAGATGGAACAGTCTCATTGCATGATGTTGAAAATGGAAAGTTGTTGAGGAGCTTGAAATCGCATACTGTGGCTGTTGTTTCTCTTAATTGGGAGGAGGATGGACAGATGACTAGAGATGAGTATGGCAGCCACTTGGTATATGAAGACCGTACTTCTCGTTTCTTTCCACCACCTCCAAGAGTTCCGCGAATGCCTGGACTGGTATCTGGAGAGACCGGTTTCATGGATGATAGTGAAGATTCATTTCGAGAGCTGTCAAATTCTTCACAGCAACGTTTTAATATCCTCTGCAGTGGAGATAAAGATGGATTCATTTGCTTTAGTATATTTGGGATCTTTCCGATAGGGAAAATTAACATACACAACTTTGGTGCTGCTAGTCCACTTAAGGATACTGAAGCTGAGTGTAGACTTCTGAATGCTTCTGTTTACAAGGTTGCTTTGTCAAAAGATCTTTGTCATTTGATAGTCATGTGTTCAGGAGAATTAATTGAAGATAGGGAAGAATCAGAAAACAGGCAGATGACTGAACCTGGTATGCATGGCTTACATTGCATAGTGCTTGATACATCTATATTTTGGAAGAGAAAAAGTGAACTTCATCAAGTGGCGCAACAAGCTTCTAACATTGAGGAATTGGCAGAGGTTATTCGGTCATCAATTTCAGTTATGCACAAGGAGTGGTCTGACGCTATGCGCACTTTTCATGACAAGTTTGATTCTCTGTCTAATCTGATCATAGACCATGGACTCCAGTCCTCTCCCCAAGAGGAATTTTTGAGTCTTTTAGGTGGTGCAAGGACTAGTCCAGCAGTTCATCAGTTTCTAGTTAACTCTCTCGGTGAAGTGGGCGTGAAGCGCGTGTCAAAGGCTGTTTGTGGTGCTGGGAAAGAACTGCAGCTTATAATCCTAAATCATCTCCAGCCTGCTGCAGAAATTATCGCATTCAGGATGGGAGAACTAAAAGGCCTTTCACGATGGAGAGCACGCTATCAGGGCATCGGTTTGGATGAGACGCTTATCAATAATGCAACAGAAAAGGCTGGAATGATACTTGTACAAGTTGAACGGTTTATCAGGGTGCTTTCATCTGTGGTGCAGCAGTTTTCGAACTTCTTCAATTGGCTCTTGAAGTGTATAAAGTTACTCAATTCAGAACCCAGTGATCATCATCTTCTACTGTACAATAGTGAACTTGTTGTTATATTCTTGAAGTTTCTATATGACCAAGATCCTGTTAAGCAGTTGCTGGAGGCATCGGAAGCTGATGAGAGTATCGAAGTTGGATTCCTGCAAAGGACGTTAGCCAAGGAATTTCAGCAGATGGAGTCTAGTTTTAAGGAGGCTTTTCAGATGCCCTTTACTACAATTTCAAGAAAGATAGTCTGTGAAGATTCATTGCCACTTTGCCCACTGCCACCTTTGTCAGCATCTTTGTCTACCAGTATTCCTATGTCAGTATCATATTATGAGGATGCCTCCCATTCCGTCCCTCGTCAGACTCGTCAACATATGTTTCTTGACTACATATCTTTCCATATACCAGATGAGTCCTTCTCTGGTATGGAAAATTGTATAGGCATAGTGAGGGGGATTATGCATGACTCGAGCATTGTAAAGAGGGGTTATACTTCAGTAGAAGCTGTACTGTTGTGCATCCCTGTTGGTTATTACTGTGTGGATCTTTCTTTATATAAGGAAAGTCAGATTGTTTTATTATTAAATGAAACAACTACCACTTCTGAAAGCTCTGGTGATGCTTGTATGATGATTGTGCAAGCAAATGACCTTCCATTTGTATCTATCACAAGATCCACTAGTCTAAACTATTGGAACTTTCATCAACTGAAGGACTCTGTTGTTCACTTGCAAATGGAGAACGAGAAGGTTCGCAGTATACCCCATTCTGTTATTGCTCCTCTAGCAGTTAGCTCATCAAGAGGTGTGGCTTGTGTTTTTGCTGCAAGAAAGCGTGCCTTGGTCTACATTCTGGAGGAAGATGAAGATGAAGTTTCTGATTCAGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0007275 GO:0008150 GO:0009790 GO:0009791 GO:0009793 GO:0009908 GO:0010154 GO:0022414 GO:0032501 GO:0032502 GO:0035670 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0048316 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048467 GO:0048481 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0061458 GO:0090567 GO:0099402

KEGG Pathways

Metabolic & Signaling

ko04110 ko04111 ko04113 ko04114 ko04120 ko04914 ko05166 map04110 map04111 map04113 map04114 map04120 map04914 map05166

Pfam Domains

Protein Families

ANAPC4 ANAPC4_WD40

Protein Analysis

758

Amino Acids

85.25

Weight (kDa)

5.3

Isoelectric Point (pI)

49.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_EML_2	PF23414	20 - 120	9.9e-09	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR36-Utp21_1st	PF25171	20 - 112	5.1e-06	WDR36/Utp21 first beta-propeller
WD40_WDHD1_1st	PF24817	22 - 116	8e-09	WDHD1 first WD40 domain
WD40_Prp19	PF24814	22 - 116	1.9e-08	Prp19 WD40 domain
Beta-prop_WDR19_1st	PF23389	22 - 118	9.1e-08	WDR19 first beta-propeller
Beta-prop_THOC3	PF25174	23 - 118	7.3e-11	THOC3 beta-propeller domain
Beta-prop_WDR35_TULP_N	PF24797	23 - 116	1.3e-07	WDR35/TULP4 N-terminal
Beta-prop_WDR5	PF25175	25 - 118	6.6e-09	WDR5 beta-propeller domain
WDR55	PF24796	26 - 132	6.4e-06	WDR55
WD40_CDC20-Fz	PF24807	27 - 134	3.6e-11	CDC20/Fizzy WD40 domain
ANAPC4_WD40	PF12894	29 - 114	3.7e-29	Anaphase-promoting complex subunit 4 WD40 domain
Beta-prop_WDR3_2nd	PF25172	33 - 117	9.3e-06	WDR3 second beta-propeller domain
Beta-prop_WDR3_1st	PF25173	63 - 118	3.4e-08	WDR3 first beta-propeller domain
ANAPC4	PF12896	266 - 468	3.3e-57	Anaphase-promoting complex, cyclosome, subunit 4

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0007288)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G21530
fragaria_vesca	FvH4_3g04050 FvH4_3g04050 FvH4_3g04050
malus_domestica	MD10G1303000.v1.1
prunus_persica	Prupe.4G036500_v2.0.a1 Prupe.4G036500_v2.0.a1 Prupe.4G036500_v2.0.a1 Prupe.4G036500_v2.0.a1 Prupe.4G036500_v2.0.a1 Prupe.4G036500_v2.0.a1
pyrus_communis	pycom10g25560
rosa_chinensis	RchiOBHm_Chr5g0006531
rosa_laevigata	RLG00000031396 RLG00000034652
rosa_multiflora	Rmu_sc0008991.1_g000016
rosa_roxburghii	Rroxscaffold_1G00031790 Rroxscaffold_1G00069530
rosa_rugosa	Rorug04G0421700
rosa_samantha	Rh5AG049400 Rh5BG048700 Rh5CG057000 Rh5DG048000
rosa_wichuraiana	Rw5G004950 Rw5G030980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1151
AasI	GACNNNNNNGTC	1 cut(s)	59
Acc16I	TGCGCA	1 cut(s)	944
Acc36I	ACCTGC	1 cut(s)	61
AccI	GTMKAC	4 cut(s)	655, 1685, 2143, 2229
AccII	CGCG	3 cut(s)	27, 433, 1107
AciI	CCGC	2 cut(s)	148, 431
AclI	AACGTT	1 cut(s)	520
AclWI	GGATC	4 cut(s)	587, 1472, 1935, 2052
AcsI	RAATTY	3 cut(s)	505, 1019, 1559
AcuI	CTGAAG	4 cut(s)	144, 663, 1863, 2114
AfaI	GTAC	4 cut(s)	397, 1305, 1430, 1893
AflII	CTTAAG	1 cut(s)	632
AflIII	ACRYGT	1 cut(s)	25
AgeI	ACCGGT	1 cut(s)	461
AgsI	TTSAA	9 cut(s)	154, 275, 301, 740, 1313, 1366, 1378, 1459, 1620
AhlI	ACTAGT	2 cut(s)	1049, 2063
AjnI	CCWGG	3 cut(s)	181, 439, 778
Alw21I	GWGCWC	1 cut(s)	1234
Alw26I	GTCTC	4 cut(s)	205, 262, 452, 1253
AlwI	GGATC	4 cut(s)	587, 1472, 1935, 2052
AlwNI	CAGNNNCTG	1 cut(s)	1495
Ama87I	CYCGRG	2 cut(s)	95, 1852
AoxI	GGCC	1 cut(s)	1213
ApeKI	GCWGC	6 cut(s)	372, 620, 1144, 1175, 1345, 2207
ApoI	RAATTY	3 cut(s)	505, 1019, 1559
ArsI	GACNNNNNNTTYG	2 cut(s)	2024, 2056
AseI	ATTAAT	1 cut(s)	734
AsiGI	ACCGGT	1 cut(s)	461
Asp700I	GAANNNNTTC	1 cut(s)	1361
AspLEI	GCGC	3 cut(s)	859, 945, 1107
AsuHPI	GGTGA	2 cut(s)	1100, 2012
AsuII	TTCGAA	1 cut(s)	1355
AvaI	CYCGRG	2 cut(s)	95, 1852
BbsI	GAAGAC	2 cut(s)	132, 396
Bbv12I	GWGCWC	1 cut(s)	1234
BbvI	GCAGC	6 cut(s)	384, 607, 1156, 1162, 1357, 2194
BccI	CCATC	8 cut(s)	212, 236, 242, 338, 545, 1192, 1218, 1564
BciT130I	CCWGG	3 cut(s)	183, 441, 780
BciVI	GTATCC	1 cut(s)	631
BclI	TGATCA	2 cut(s)	981, 1411
BcoDI	GTCTC	4 cut(s)	205, 262, 452, 1253
BcuI	ACTAGT	2 cut(s)	1049, 2063
BfaI	CTAG	7 cut(s)	357, 624, 1050, 1073, 1578, 2064, 2168
BfmI	CTRYAG	3 cut(s)	535, 1142, 1176
BfrI	CTTAAG	1 cut(s)	632
BfuAI	ACCTGC	1 cut(s)	61
BfuI	GTATCC	1 cut(s)	631
BglII	AGATCT	1 cut(s)	697
BisI	GCNGC	6 cut(s)	373, 621, 1145, 1176, 1346, 2208
BlsI	GCNGC	6 cut(s)	374, 622, 1146, 1177, 1347, 2209
Bme1390I	CCNGG	3 cut(s)	183, 441, 780
BmeT110I	CYCGRG	2 cut(s)	95, 1852
BmrFI	CCNGG	3 cut(s)	183, 441, 780
BmrI	ACTGGG	1 cut(s)	1401
BmsI	GCATC	7 cut(s)	1254, 1511, 1590, 1685, 1711, 1911, 1993
BmuI	ACTGGG	1 cut(s)	1401
BoxI	GACNNNNGTC	1 cut(s)	1000
BpiI	GAAGAC	2 cut(s)	132, 396
BplI	GAGNNNNNCTC	4 cut(s)	1709, 1741, 1787, 1819
BpmI	CTGGAG	6 cut(s)	29, 474, 983, 1151, 1517, 2258
Bpu14I	TTCGAA	1 cut(s)	1355
BsaBI	GATNNNNATC	1 cut(s)	1416
BsaI	GGTCTC	2 cut(s)	205, 452
BsaJI	CCNNGG	6 cut(s)	114, 181, 182, 991, 1554, 2223
BsaWI	WCCGGW	1 cut(s)	461
Bse118I	RCCGGY	1 cut(s)	461
Bse1I	ACTGG	6 cut(s)	46, 59, 450, 1000, 1407, 1689
Bse3DI	GCAATG	3 cut(s)	260, 796, 1643
Bse8I	GATNNNNATC	1 cut(s)	1416
BseBI	CCWGG	3 cut(s)	183, 441, 780
BseDI	CCNNGG	6 cut(s)	114, 181, 182, 991, 1554, 2223
BseGI	GGATG	6 cut(s)	349, 478, 1203, 1261, 1726, 1902
BseJI	GATNNNNATC	1 cut(s)	1416
BseMI	GCAATG	3 cut(s)	260, 796, 1643
BseMII	CTCAG	1 cut(s)	639
BseNI	ACTGG	6 cut(s)	46, 59, 450, 1000, 1407, 1689
BseRI	GAGGAG	3 cut(s)	307, 353, 2154
BseXI	GCAGC	6 cut(s)	384, 607, 1156, 1162, 1357, 2194
BseYI	CCCAGC	1 cut(s)	1130
BsgI	GTGCAG	2 cut(s)	128, 1364
Bsh1236I	CGCG	3 cut(s)	27, 433, 1107
BshFI	GGCC	1 cut(s)	1215
BshTI	ACCGGT	1 cut(s)	461
BsiHKAI	GWGCWC	1 cut(s)	1234
BsiHKCI	CYCGRG	2 cut(s)	95, 1852
BsiSI	CCGG	1 cut(s)	462
BslFI	GGGAC	1 cut(s)	1721
BsmAI	GTCTC	4 cut(s)	205, 262, 452, 1253
BsmBI	CGTCTC	1 cut(s)	1253
BsmFI	GGGAC	1 cut(s)	1721
BsmI	GAATGC	3 cut(s)	441, 670, 1190
BsnI	GGCC	1 cut(s)	1215
Bso31I	GGTCTC	2 cut(s)	205, 452
BsoBI	CYCGRG	2 cut(s)	95, 1852
Bsp119I	TTCGAA	1 cut(s)	1355
Bsp1286I	GDGCHC	1 cut(s)	1234
Bsp1407I	TGTACA	2 cut(s)	1303, 1428
Bsp143I	GATC	8 cut(s)	78, 579, 697, 981, 1411, 1477, 1927, 2057
Bsp19I	CCATGG	2 cut(s)	114, 991
BspACI	CCGC	2 cut(s)	148, 431
BspANI	GGCC	1 cut(s)	1215
BspCNI	CTCAG	1 cut(s)	640
BspFNI	CGCG	3 cut(s)	27, 433, 1107
BspHI	TCATGA	1 cut(s)	952
BspMAI	CTGCAG	3 cut(s)	539, 1146, 1180
BspMI	ACCTGC	1 cut(s)	61
BspPI	GGATC	4 cut(s)	587, 1472, 1935, 2052
BspQI	GCTCTTC	1 cut(s)	182
BspT104I	TTCGAA	1 cut(s)	1355
BspTI	CTTAAG	1 cut(s)	632
BspTNI	GGTCTC	2 cut(s)	205, 452
BsrDI	GCAATG	3 cut(s)	260, 796, 1643
BsrFI	RCCGGY	1 cut(s)	461
BsrGI	TGTACA	2 cut(s)	1303, 1428
BsrI	ACTGG	6 cut(s)	46, 59, 450, 1000, 1407, 1689
BssAI	RCCGGY	1 cut(s)	461
BssECI	CCNNGG	6 cut(s)	114, 181, 182, 991, 1554, 2223
BssMI	GATC	8 cut(s)	78, 579, 697, 981, 1411, 1477, 1927, 2057
BssNAI	GTATAC	1 cut(s)	2144
BssT1I	CCWWGG	4 cut(s)	114, 991, 1554, 2223
Bst1107I	GTATAC	1 cut(s)	2144
Bst2UI	CCWGG	3 cut(s)	183, 441, 780
Bst4CI	ACNGT	7 cut(s)	256, 313, 395, 1317, 1428, 1896, 1922
Bst6I	CTCTTC	2 cut(s)	182, 824
BstAFI	CTTAAG	1 cut(s)	632
BstAUI	TGTACA	2 cut(s)	1303, 1428
BstBI	TTCGAA	1 cut(s)	1355
BstC8I	GCNNGC	4 cut(s)	1173, 1234, 2025, 2220
BstDEI	CTNAG	1 cut(s)	648
BstDSI	CCRYGG	2 cut(s)	114, 991
BstF5I	GGATG	6 cut(s)	349, 478, 1203, 1261, 1726, 1902
BstFNI	CGCG	3 cut(s)	27, 433, 1107
BstHHI	GCGC	3 cut(s)	859, 945, 1107
BstKTI	GATC	8 cut(s)	81, 582, 700, 984, 1414, 1480, 1930, 2060
BstMAI	GTCTC	4 cut(s)	205, 262, 452, 1253
BstMBI	GATC	8 cut(s)	78, 579, 697, 981, 1411, 1477, 1927, 2057
BstMWI	GCNNNNNNNGC	6 cut(s)	798, 863, 1104, 1242, 1508, 2168
BstNI	CCWGG	3 cut(s)	183, 441, 780
BstPAI	GACNNNNGTC	1 cut(s)	1000
BstSCI	CCNGG	3 cut(s)	181, 439, 778
BstSFI	CTRYAG	3 cut(s)	535, 1142, 1176
BstUI	CGCG	3 cut(s)	27, 433, 1107
BstV1I	GCAGC	6 cut(s)	384, 607, 1156, 1162, 1357, 2194
BstV2I	GAAGAC	2 cut(s)	132, 396
BstX2I	RGATCY	5 cut(s)	579, 697, 1477, 1927, 2057
BstYI	RGATCY	5 cut(s)	579, 697, 1477, 1927, 2057
BstZ17I	GTATAC	1 cut(s)	2144
BsuI	GTATCC	1 cut(s)	631
BsuRI	GGCC	1 cut(s)	1215
BtgI	CCRYGG	2 cut(s)	114, 991
BtgZI	GCGATG	1 cut(s)	214
BtsCI	GGATG	6 cut(s)	349, 478, 1203, 1261, 1726, 1902
BtsI	GCAGTG	2 cut(s)	544, 1658
BtsIMutI	CAGTG	3 cut(s)	544, 1414, 1658
BveI	ACCTGC	1 cut(s)	61
Cac8I	GCNNGC	4 cut(s)	1173, 1234, 2025, 2220
CaiI	CAGNNNCTG	1 cut(s)	1495
CciI	TCATGA	1 cut(s)	952
CfoI	GCGC	3 cut(s)	859, 945, 1107
Cfr10I	RCCGGY	1 cut(s)	461
CseI	GACGC	3 cut(s)	16, 944, 1270
CsiI	ACCWGGT	1 cut(s)	778
Csp6I	GTAC	4 cut(s)	396, 1304, 1429, 1892
CspAI	ACCGGT	1 cut(s)	461
CspCI	CAANNNNNGTGG	2 cut(s)	2172, 2207
CviAII	CATG	8 cut(s)	115, 266, 469, 718, 788, 953, 992, 1847
CviQI	GTAC	4 cut(s)	396, 1304, 1429, 1892
DdeI	CTNAG	1 cut(s)	648
DpnI	GATC	8 cut(s)	80, 581, 699, 983, 1413, 1479, 1929, 2059
DpnII	GATC	8 cut(s)	78, 579, 697, 981, 1411, 1477, 1927, 2057
DrdI	GACNNNNNNGTC	1 cut(s)	59
DseDI	GACNNNNNNGTC	1 cut(s)	59
Eam1104I	CTCTTC	2 cut(s)	182, 824
EarI	CTCTTC	2 cut(s)	182, 824
Eco130I	CCWWGG	4 cut(s)	114, 991, 1554, 2223
Eco147I	AGGCCT	1 cut(s)	1215
Eco31I	GGTCTC	2 cut(s)	205, 452
Eco57I	CTGAAG	4 cut(s)	144, 663, 1863, 2114
Eco88I	CYCGRG	2 cut(s)	95, 1852
EcoRII	CCWGG	3 cut(s)	181, 439, 778
EcoT14I	CCWWGG	4 cut(s)	114, 991, 1554, 2223
EcoT22I	ATGCAT	2 cut(s)	789, 1848
ErhI	CCWWGG	4 cut(s)	114, 991, 1554, 2223
Esp3I	CGTCTC	1 cut(s)	1253
FaeI	CATG	8 cut(s)	118, 269, 472, 721, 791, 956, 995, 1850
FalI	AAGNNNNNCTT	2 cut(s)	2207, 2239
FaqI	GGGAC	1 cut(s)	1721
FatI	CATG	8 cut(s)	114, 265, 468, 717, 787, 952, 991, 1846
FauNDI	CATATG	1 cut(s)	1758
FbaI	TGATCA	2 cut(s)	981, 1411
FblI	GTMKAC	4 cut(s)	655, 1685, 2143, 2229
Fnu4HI	GCNGC	6 cut(s)	373, 621, 1145, 1176, 1346, 2208
FokI	GGATG	6 cut(s)	356, 485, 1210, 1268, 1733, 1889
Fsp4HI	GCNGC	6 cut(s)	373, 621, 1145, 1176, 1346, 2208
FspAI	RTGCGCAY	1 cut(s)	944
FspBI	CTAG	7 cut(s)	357, 624, 1050, 1073, 1578, 2064, 2168
FspI	TGCGCA	1 cut(s)	944
GlaI	GCGC	3 cut(s)	858, 944, 1106
GluI	GCNGC	6 cut(s)	373, 621, 1145, 1176, 1346, 2208
GsaI	CCCAGC	1 cut(s)	1134
GsuI	CTGGAG	6 cut(s)	29, 474, 983, 1151, 1517, 2258
HaeIII	GGCC	1 cut(s)	1215
HapII	CCGG	1 cut(s)	462
HgaI	GACGC	3 cut(s)	16, 944, 1270
HhaI	GCGC	3 cut(s)	859, 945, 1107
Hin1II	CATG	8 cut(s)	118, 269, 472, 721, 791, 956, 995, 1850
Hin6I	GCGC	3 cut(s)	857, 943, 1105
HinP1I	GCGC	3 cut(s)	857, 943, 1105
HincII	GTYRAC	2 cut(s)	1078, 1754
HindII	GTYRAC	2 cut(s)	1078, 1754
HindIII	AAGCTT	1 cut(s)	864
HpaI	GTTAAC	1 cut(s)	1078
HpaII	CCGG	1 cut(s)	462
HphI	GGTGA	2 cut(s)	1100, 2012
HpyAV	CCTTC	5 cut(s)	94, 1810, 2045, 2089, 2125
HpyCH4III	ACNGT	7 cut(s)	256, 313, 395, 1317, 1428, 1896, 1922
HpyCH4IV	ACGT	3 cut(s)	197, 520, 1547
HpyF10VI	GCNNNNNNNGC	6 cut(s)	798, 863, 1104, 1242, 1508, 2168
HpyF3I	CTNAG	1 cut(s)	648
HpySE526I	ACGT	3 cut(s)	197, 520, 1547
Hsp92II	CATG	8 cut(s)	118, 269, 472, 721, 791, 956, 995, 1850
HspAI	GCGC	3 cut(s)	857, 943, 1105
Ksp22I	TGATCA	2 cut(s)	981, 1411
KspAI	GTTAAC	1 cut(s)	1078
Kzo9I	GATC	8 cut(s)	78, 579, 697, 981, 1411, 1477, 1927, 2057
LguI	GCTCTTC	1 cut(s)	182
LmnI	GCTCC	3 cut(s)	48, 294, 2167
Lsp1109I	GCAGC	6 cut(s)	384, 607, 1156, 1162, 1357, 2194
LweI	GCATC	7 cut(s)	1254, 1511, 1590, 1685, 1711, 1911, 1993
MabI	ACCWGGT	1 cut(s)	778
MaeI	CTAG	7 cut(s)	357, 624, 1050, 1073, 1578, 2064, 2168
MaeII	ACGT	3 cut(s)	197, 520, 1547
MaeIII	GTNAC	3 cut(s)	28, 118, 1389
MalI	GATC	8 cut(s)	80, 581, 699, 983, 1413, 1479, 1929, 2059
MboI	GATC	8 cut(s)	78, 579, 697, 981, 1411, 1477, 1927, 2057
MfeI	CAATTG	2 cut(s)	154, 1366
MflI	RGATCY	5 cut(s)	579, 697, 1477, 1927, 2057
MhlI	GDGCHC	1 cut(s)	1234
MluI	ACGCGT	1 cut(s)	25
MlyI	GAGTC	8 cut(s)	122, 990, 1036, 1583, 1741, 1805, 1844, 2093
MmeI	TCCRAC	2 cut(s)	188, 1510
Mph1103I	ATGCAT	2 cut(s)	789, 1848
MroXI	GAANNNNTTC	1 cut(s)	1361
MspCI	CTTAAG	1 cut(s)	632
MspI	CCGG	1 cut(s)	462
MspR9I	CCNGG	3 cut(s)	183, 441, 780
MunI	CAATTG	2 cut(s)	154, 1366
Mva1269I	GAATGC	3 cut(s)	441, 670, 1190
MvaI	CCWGG	3 cut(s)	183, 441, 780
MvnI	CGCG	3 cut(s)	27, 433, 1107
MwoI	GCNNNNNNNGC	6 cut(s)	798, 863, 1104, 1242, 1508, 2168
NcoI	CCATGG	2 cut(s)	114, 991
NdeI	CATATG	1 cut(s)	1758
NdeII	GATC	8 cut(s)	78, 579, 697, 981, 1411, 1477, 1927, 2057
NlaIII	CATG	8 cut(s)	118, 269, 472, 721, 791, 956, 995, 1850
NmuCI	GTSAC	1 cut(s)	28
NsbI	TGCGCA	1 cut(s)	944
NsiI	ATGCAT	2 cut(s)	789, 1848
NspV	TTCGAA	1 cut(s)	1355
PaeR7I	CTCGAG	1 cut(s)	1852
PagI	TCATGA	1 cut(s)	952
PasI	CCCWGGG	1 cut(s)	182
PceI	AGGCCT	1 cut(s)	1215
PciSI	GCTCTTC	1 cut(s)	182
PcsI	WCGNNNNNNNCGW	1 cut(s)	1747
PctI	GAATGC	3 cut(s)	441, 670, 1190
PdmI	GAANNNNTTC	1 cut(s)	1361
PfeI	GAWTC	8 cut(s)	91, 485, 555, 752, 965, 1533, 1640, 2264
PinAI	ACCGGT	1 cut(s)	461
PkrI	GCNGC	6 cut(s)	374, 622, 1146, 1177, 1347, 2209
PleI	GAGTC	8 cut(s)	122, 990, 1035, 1582, 1741, 1804, 1844, 2093
PpsI	GAGTC	8 cut(s)	122, 990, 1035, 1582, 1741, 1804, 1844, 2093
PshAI	GACNNNNGTC	1 cut(s)	1000
PshBI	ATTAAT	1 cut(s)	734
PsiI	TTATAA	1 cut(s)	1151
Psp1406I	AACGTT	1 cut(s)	520
Psp6I	CCWGG	3 cut(s)	181, 439, 778
PspFI	CCCAGC	1 cut(s)	1130
PspGI	CCWGG	3 cut(s)	181, 439, 778
PspXI	VCTCGAGB	1 cut(s)	1852
PstI	CTGCAG	3 cut(s)	539, 1146, 1180
PstNI	CAGNNNCTG	1 cut(s)	1495
PsuI	RGATCY	5 cut(s)	579, 697, 1477, 1927, 2057
RsaI	GTAC	4 cut(s)	397, 1305, 1430, 1893
RsaNI	GTAC	4 cut(s)	396, 1304, 1429, 1892
SapI	GCTCTTC	1 cut(s)	182
SatI	GCNGC	6 cut(s)	373, 621, 1145, 1176, 1346, 2208
Sau3AI	GATC	8 cut(s)	78, 579, 697, 981, 1411, 1477, 1927, 2057
SchI	GAGTC	8 cut(s)	122, 990, 1036, 1583, 1741, 1805, 1844, 2093
ScrFI	CCNGG	3 cut(s)	183, 441, 780
SduI	GDGCHC	1 cut(s)	1234
SexAI	ACCWGGT	1 cut(s)	778
SfaNI	GCATC	7 cut(s)	1254, 1511, 1590, 1685, 1711, 1911, 1993
SfcI	CTRYAG	3 cut(s)	535, 1142, 1176
Sfr274I	CTCGAG	1 cut(s)	1852
SfuI	TTCGAA	1 cut(s)	1355
SlaI	CTCGAG	1 cut(s)	1852
SmlI	CTYRAG	2 cut(s)	632, 1852
SmoI	CTYRAG	2 cut(s)	632, 1852
SpeI	ACTAGT	2 cut(s)	1049, 2063
SseBI	AGGCCT	1 cut(s)	1215
SsiI	CCGC	2 cut(s)	148, 431
SspMI	CTAG	7 cut(s)	357, 624, 1050, 1073, 1578, 2064, 2168
StuI	AGGCCT	1 cut(s)	1215
StyD4I	CCNGG	3 cut(s)	181, 439, 778
StyI	CCWWGG	4 cut(s)	114, 991, 1554, 2223
TaaI	ACNGT	7 cut(s)	256, 313, 395, 1317, 1428, 1896, 1922
TaiI	ACGT	3 cut(s)	200, 523, 1550
TaqI	TCGA	5 cut(s)	51, 493, 1355, 1524, 1853
TaqII	GACCGA	1 cut(s)	888
TatI	WGTACW	3 cut(s)	1303, 1428, 1891
TfiI	GAWTC	8 cut(s)	91, 485, 555, 752, 965, 1533, 1640, 2264
TscAI	CASTG	3 cut(s)	544, 1414, 1665
TseFI	GTSAC	1 cut(s)	28
TseI	GCWGC	6 cut(s)	372, 620, 1144, 1175, 1345, 2207
Tsp45I	GTSAC	1 cut(s)	28
TspGWI	ACGGA	1 cut(s)	1723
TspRI	CASTG	3 cut(s)	544, 1414, 1665
Vha464I	CTTAAG	1 cut(s)	632
VspI	ATTAAT	1 cut(s)	734
XapI	RAATTY	3 cut(s)	505, 1019, 1559
XhoI	CTCGAG	1 cut(s)	1852
XmiI	GTMKAC	4 cut(s)	655, 1685, 2143, 2229
XmnI	GAANNNNTTC	1 cut(s)	1361
XspI	CTAG	7 cut(s)	357, 624, 1050, 1073, 1578, 2064, 2168
Zsp2I	ATGCAT	2 cut(s)	789, 1848

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.