Rw5G004950

Anaphase-promoting complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0007288
Physical Location & Seq
Forward (+)
5143575 .. 5149927
6353 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G004950.1

Sequence Viewer

Length: 2334 bp
ATGGAAACCGACGAAGAAGAACAACGCGTCACTCCGTTCCAGCTCCAGTTCGACAAGCCAGTCGCTTCGCAGATTAAGATCGCGGAGTGGAATCCCGAGAAGGATTTACTCGCCATGGTAACTGAAGACTCCAAGATTTTACTGCACCGCTTCAATTGGCAGAGGCTCTGGACAATCTCCCCAGGGAAGAGCATAACGTCTTTGTGTTGGAGACCTGATGGTAAAGCCATCGCGGTCGGGCTCGAAGATGGAACAGTCTCATTGCATGATGTTGAAAATGGAAAGTTGTTGAGGAGCTTGAAATCACATACTGTGGCTGTTGTTTCTCTTAATTGGGAGGAGGATGGACAGATGACCAGAGATGAGTATGGCAGCCACTCGGTATATGAAGACCGTACTTCTCGTTTCTTTCCACCACCTCCAAGAGTTCCGCGAATGCCTGGACTGGTATCTGGAGAGACCGGTTTCATGGACGATAGTGAAGATTCATTTCGAGAGCTGTCAAATTCTTCACAGCAACGTTTTAATATCCTCTGCAGTGGAGATAAAGATGGATTCATTTGCTTTAGTATATTTGGGATCTTTCCGATAGGGAAAATTAACATACACAACTTTGGTGCTGCTAGTCCACTTAAGGATACTGAAGCTGAGTGTAGACTTCTGAATGCTTCTGTTTACAAGGTTGCTTTGTCAAAAGATCTTTGTCATTTGATAGTCATGTGTTCAGGAGAATTAATTGAAGATAGGGAAGAATCAGAAAACAGGCAGATGACTGAACCTGGTATGCATGGCTTACATTGCATAGTGCTTGATACATCTATATTTTGGACGAGAAAAAGTGAGCTTCATCAAGTGGCGCAACAAGCTTCTAACATTGAGGAATTGGCAGAGGTTATTCGATCATCAATTTCAGTTATGCACAAGGAGTGGTCTGATGCTATGCGCACTTTTCATGACAAGTTTGATTCTCTGTCCAATCTGATCATAGACCATGGACTCCAGTCCTCTCCCCAAGAGGAGTTTTTGAGTCTTTTAGGCGGTGCAAGGACTAGTCCAGCAGTTCATCAGTTTCTAGTTAACTCTCTCGGTGAAGTGGGCGTGAAGCGCGTGTCAAAGGCTGTTTGTGGTGCTGGGAAAGAACTGCAGCTTATAATCCTAAATCATCTCCAGCCTGCTGCAGAAATTATCGCATTCAGGATGGGAGAACTAAGAGGCCTTTCAAGATGGAGAGCACGCTATCAGGGCATCGGTTTGGATGAGACGCTTATCAATAATGCAACAGAAAAAGCTGGAATGATACTTGTACAAGTTGAACGGTTTATCAGGGTGCTTTCATCTGTGGTGCAGCAGTTTTCGAACTTCTTCAATTGGCTCTTGAAGTGTATAAAGTTACTCAATTCAGAACCCAGTGATCATCATCTTCTACTGTACAATAGTGAACTTGTTGTTATATTCTTGAAGTTCCTATATGACCAAGATCCTGTTAAGCAGTTGCTGGAGGCGTCGGAAGCTGATGAGAGTATCGAAGTTGGATTGGAAACAGTGCAGAGAGTTAGAGAATTAGTTCAGTTTGGGGGATTTTCAGATTGTGAGCACCTGCAGAGGACGTTAGCCAAGGAATTTCAGCAGATGGAGTCTAGTTTTAAGGAGGCTTTTCAGATGCCCTTTATTACAATTTCAAGAAAGATAGTCTGTGAAGATTCATTGCCACTTTGCCCACTGCCACCTTCGTCAGCATCTTTGTCTACCAGTATTCCTATGTCAGTATCATATTATGAGGATGCCTCCCATTCCGTCCCTCATCAGACTCGTCAACATATGTTTCTTGACTACATATCTTTCCATATACCAGATGAGTCCTTCTCTGGTATGGAAAATTGTATAGGCATAGTGAGGGGGATTATGCATGACTCAAGCATTGTAAAGAGGGGTTATACTTCAGTAGAAGCTGTACTGTTGTGCATCCCTGTTGGTTATTACTGTGTGGATCTTTCTTTATATAAGGAAAGTCAGATTGTTTTATTATTAAATGAAACAACTACCACTTCTGAAAGCTCTGGTGATGCCTGTATGATGGTTGTGCAAGCAAATGACCTTCCATTTGTATCTATCACAAGATCCACTAGTCTATACTATTGGAACTTGCATCAACTGAAGGACTCTGTTGTTCACTTGCAAATGGAGAACGAGAAGGTTCGCAGTATACCCCATTCTGTTATTGCTCCTCTAGCAGTTAGCTCATCAAGAGGTGTGGCTTGTGTTTTTGCTGCAAGAAAGCGTGCCTTGGTCTACATTCTGGAGGAAGATGAAGATGAAGTTTCTGATTCAGAGTGA

Protein Analysis

777

Amino Acids

87.5

Weight (kDa)

5.18

Isoelectric Point (pI)

49.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_EML_2 PF23414 20 - 120 1.2e-08 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 20 - 112 4.8e-06 WDR36/Utp21 first beta-propeller
WD40_WDHD1_1st PF24817 22 - 115 8e-09 WDHD1 first WD40 domain
Beta-prop_WDR19_1st PF23389 22 - 118 1.8e-07 WDR19 first beta-propeller
WD40_Prp19 PF24814 23 - 116 2.9e-08 Prp19 WD40 domain
Beta-prop_WDR35_TULP_N PF24797 23 - 114 4.1e-07 WDR35/TULP4 N-terminal
Beta-prop_THOC3 PF25174 24 - 118 1e-10 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 25 - 118 7e-09 WDR5 beta-propeller domain
WD40_CDC20-Fz PF24807 27 - 132 5.6e-11 CDC20/Fizzy WD40 domain
WDR55 PF24796 27 - 134 3.4e-06 WDR55
ANAPC4_WD40 PF12894 29 - 114 3.9e-29 Anaphase-promoting complex subunit 4 WD40 domain
Beta-prop_WDR3_2nd PF25172 34 - 118 8.6e-06 WDR3 second beta-propeller domain
Beta-prop_WDR3_1st PF25173 63 - 118 3.3e-08 WDR3 first beta-propeller domain
ANAPC4 PF12896 266 - 468 4.9e-57 Anaphase-promoting complex, cyclosome, subunit 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1151
AarI CACCTGC 1 cut(s) 1605
AasI GACNNNNNNGTC 1 cut(s) 59
Acc16I TGCGCA 1 cut(s) 944
Acc36I ACCTGC 1 cut(s) 1605
AccI GTMKAC 4 cut(s) 655, 1745, 2203, 2289
AccII CGCG 5 cut(s) 27, 83, 233, 433, 1107
AciI CCGC 5 cut(s) 83, 148, 233, 431, 1038
AclI AACGTT 1 cut(s) 520
AclWI GGATC 4 cut(s) 587, 1472, 1995, 2112
AcsI RAATTY 2 cut(s) 505, 1619
AcuI CTGAAG 4 cut(s) 144, 663, 1923, 2174
AcyI GRCGYC 1 cut(s) 1502
AfaI GTAC 4 cut(s) 397, 1305, 1430, 1953
AflII CTTAAG 1 cut(s) 632
AflIII ACRYGT 1 cut(s) 25
AgeI ACCGGT 1 cut(s) 461
AhlI ACTAGT 2 cut(s) 1049, 2123
AjnI CCWGG 3 cut(s) 181, 439, 778
Alw21I GWGCWC 2 cut(s) 1234, 1596
Alw26I GTCTC 4 cut(s) 205, 262, 452, 1253
AlwI GGATC 4 cut(s) 587, 1472, 1995, 2112
AlwNI CAGNNNCTG 1 cut(s) 1495
Ama87I CYCGRG 1 cut(s) 95
AoxI GGCC 1 cut(s) 1213
ApeKI GCWGC 6 cut(s) 372, 620, 1144, 1175, 1345, 2267
ApoI RAATTY 2 cut(s) 505, 1619
ArsI GACNNNNNNTTYG 2 cut(s) 2084, 2116
AseI ATTAAT 1 cut(s) 734
AsiGI ACCGGT 1 cut(s) 461
Asp700I GAANNNNTTC 2 cut(s) 1361, 1563
AspLEI GCGC 3 cut(s) 859, 945, 1107
AsuHPI GGTGA 2 cut(s) 1100, 2072
AsuII TTCGAA 1 cut(s) 1355
AvaI CYCGRG 1 cut(s) 95
BanII GRGCYC 1 cut(s) 243
BbsI GAAGAC 2 cut(s) 132, 396
Bbv12I GWGCWC 2 cut(s) 1234, 1596
BbvI GCAGC 6 cut(s) 384, 607, 1156, 1162, 1357, 2254
BccI CCATC 9 cut(s) 212, 236, 242, 338, 545, 1192, 1218, 1624, 2068
BciT130I CCWGG 3 cut(s) 183, 441, 780
BciVI GTATCC 1 cut(s) 631
BclI TGATCA 2 cut(s) 981, 1411
BcoDI GTCTC 4 cut(s) 205, 262, 452, 1253
BcuI ACTAGT 2 cut(s) 1049, 2123
BfaI CTAG 6 cut(s) 624, 1050, 1073, 1638, 2124, 2228
BfmI CTRYAG 4 cut(s) 535, 1142, 1176, 1598
BfrI CTTAAG 1 cut(s) 632
BfuAI ACCTGC 1 cut(s) 1605
BfuI GTATCC 1 cut(s) 631
BglII AGATCT 1 cut(s) 697
BisI GCNGC 6 cut(s) 373, 621, 1145, 1176, 1346, 2268
BlsI GCNGC 6 cut(s) 374, 622, 1146, 1177, 1347, 2269
Bme1390I CCNGG 3 cut(s) 183, 441, 780
BmeT110I CYCGRG 1 cut(s) 95
BmrFI CCNGG 3 cut(s) 183, 441, 780
BmrI ACTGGG 1 cut(s) 1401
BmsI GCATC 8 cut(s) 925, 1254, 1650, 1745, 1771, 1971, 2053, 2155
BmuI ACTGGG 1 cut(s) 1401
BoxI GACNNNNGTC 1 cut(s) 1000
BpiI GAAGAC 2 cut(s) 132, 396
BplI GAGNNNNNCTC 4 cut(s) 1769, 1801, 1847, 1879
BpmI CTGGAG 6 cut(s) 29, 474, 983, 1151, 1517, 2318
Bpu14I TTCGAA 1 cut(s) 1355
BpuEI CTTGAG 1 cut(s) 1897
BsaBI GATNNNNATC 2 cut(s) 77, 1416
BsaHI GRCGYC 1 cut(s) 1502
BsaI GGTCTC 2 cut(s) 205, 452
BsaJI CCNNGG 6 cut(s) 114, 181, 182, 991, 1614, 2283
BsaWI WCCGGW 1 cut(s) 461
Bse118I RCCGGY 1 cut(s) 461
Bse1I ACTGG 6 cut(s) 46, 59, 450, 1000, 1407, 1749
Bse3DI GCAATG 3 cut(s) 260, 796, 1703
Bse8I GATNNNNATC 2 cut(s) 77, 1416
BseBI CCWGG 3 cut(s) 183, 441, 780
BseDI CCNNGG 6 cut(s) 114, 181, 182, 991, 1614, 2283
BseGI GGATG 5 cut(s) 349, 1203, 1261, 1786, 1962
BseJI GATNNNNATC 2 cut(s) 77, 1416
BseMI GCAATG 3 cut(s) 260, 796, 1703
BseMII CTCAG 1 cut(s) 639
BseNI ACTGG 6 cut(s) 46, 59, 450, 1000, 1407, 1749
BseRI GAGGAG 4 cut(s) 307, 353, 1031, 2214
BseXI GCAGC 6 cut(s) 384, 607, 1156, 1162, 1357, 2254
BseYI CCCAGC 1 cut(s) 1130
BsgI GTGCAG 3 cut(s) 128, 1364, 1565
Bsh1236I CGCG 5 cut(s) 27, 83, 233, 433, 1107
Bsh1285I CGRYCG 1 cut(s) 237
BshFI GGCC 1 cut(s) 1215
BshTI ACCGGT 1 cut(s) 461
BsiEI CGRYCG 1 cut(s) 237
BsiHKAI GWGCWC 2 cut(s) 1234, 1596
BsiHKCI CYCGRG 1 cut(s) 95
BsiSI CCGG 1 cut(s) 462
BslFI GGGAC 1 cut(s) 1781
BsmAI GTCTC 4 cut(s) 205, 262, 452, 1253
BsmBI CGTCTC 1 cut(s) 1253
BsmFI GGGAC 1 cut(s) 1781
BsmI GAATGC 3 cut(s) 441, 670, 1190
BsnI GGCC 1 cut(s) 1215
Bso31I GGTCTC 2 cut(s) 205, 452
BsoBI CYCGRG 1 cut(s) 95
Bsp119I TTCGAA 1 cut(s) 1355
Bsp1286I GDGCHC 3 cut(s) 243, 1234, 1596
Bsp1407I TGTACA 2 cut(s) 1303, 1428
Bsp143I GATC 9 cut(s) 78, 579, 697, 899, 981, 1411, 1477, 1987, 2117
Bsp19I CCATGG 2 cut(s) 114, 991
BspACI CCGC 5 cut(s) 83, 148, 233, 431, 1038
BspANI GGCC 1 cut(s) 1215
BspCNI CTCAG 1 cut(s) 640
BspFNI CGCG 5 cut(s) 27, 83, 233, 433, 1107
BspHI TCATGA 1 cut(s) 952
BspMAI CTGCAG 4 cut(s) 539, 1146, 1180, 1602
BspMI ACCTGC 1 cut(s) 1605
BspPI GGATC 4 cut(s) 587, 1472, 1995, 2112
BspQI GCTCTTC 1 cut(s) 182
BspT104I TTCGAA 1 cut(s) 1355
BspTI CTTAAG 1 cut(s) 632
BspTNI GGTCTC 2 cut(s) 205, 452
BsrDI GCAATG 3 cut(s) 260, 796, 1703
BsrFI RCCGGY 1 cut(s) 461
BsrGI TGTACA 2 cut(s) 1303, 1428
BsrI ACTGG 6 cut(s) 46, 59, 450, 1000, 1407, 1749
BssAI RCCGGY 1 cut(s) 461
BssECI CCNNGG 6 cut(s) 114, 181, 182, 991, 1614, 2283
BssMI GATC 9 cut(s) 78, 579, 697, 899, 981, 1411, 1477, 1987, 2117
BssNAI GTATAC 1 cut(s) 2204
BssNI GRCGYC 1 cut(s) 1502
BssT1I CCWWGG 4 cut(s) 114, 991, 1614, 2283
Bst1107I GTATAC 1 cut(s) 2204
Bst2UI CCWGG 3 cut(s) 183, 441, 780
Bst4CI ACNGT 8 cut(s) 256, 313, 395, 1317, 1428, 1543, 1956, 1982
Bst6I CTCTTC 1 cut(s) 182
BstACI GRCGYC 1 cut(s) 1502
BstAFI CTTAAG 1 cut(s) 632
BstAUI TGTACA 2 cut(s) 1303, 1428
BstBI TTCGAA 1 cut(s) 1355
BstC8I GCNNGC 4 cut(s) 1173, 1234, 2085, 2280
BstDEI CTNAG 2 cut(s) 648, 1208
BstDSI CCRYGG 2 cut(s) 114, 991
BstF5I GGATG 5 cut(s) 349, 1203, 1261, 1786, 1962
BstFNI CGCG 5 cut(s) 27, 83, 233, 433, 1107
BstHHI GCGC 3 cut(s) 859, 945, 1107
BstKTI GATC 9 cut(s) 81, 582, 700, 902, 984, 1414, 1480, 1990, 2120
BstMAI GTCTC 4 cut(s) 205, 262, 452, 1253
BstMBI GATC 9 cut(s) 78, 579, 697, 899, 981, 1411, 1477, 1987, 2117
BstMCI CGRYCG 1 cut(s) 237
BstMWI GCNNNNNNNGC 6 cut(s) 798, 863, 1104, 1242, 1508, 2228
BstNI CCWGG 3 cut(s) 183, 441, 780
BstPAI GACNNNNGTC 1 cut(s) 1000
BstSCI CCNGG 3 cut(s) 181, 439, 778
BstSFI CTRYAG 4 cut(s) 535, 1142, 1176, 1598
BstUI CGCG 5 cut(s) 27, 83, 233, 433, 1107
BstV1I GCAGC 6 cut(s) 384, 607, 1156, 1162, 1357, 2254
BstV2I GAAGAC 2 cut(s) 132, 396
BstX2I RGATCY 5 cut(s) 579, 697, 1477, 1987, 2117
BstYI RGATCY 5 cut(s) 579, 697, 1477, 1987, 2117
BstZ17I GTATAC 1 cut(s) 2204
BsuI GTATCC 1 cut(s) 631
BsuRI GGCC 1 cut(s) 1215
BtgI CCRYGG 2 cut(s) 114, 991
BtgZI GCGATG 1 cut(s) 214
BtsCI GGATG 5 cut(s) 349, 1203, 1261, 1786, 1962
BtsI GCAGTG 2 cut(s) 544, 1718
BtsIMutI CAGTG 4 cut(s) 544, 1414, 1548, 1718
BveI ACCTGC 1 cut(s) 1605
Cac8I GCNNGC 4 cut(s) 1173, 1234, 2085, 2280
CaiI CAGNNNCTG 1 cut(s) 1495
CciI TCATGA 1 cut(s) 952
CfoI GCGC 3 cut(s) 859, 945, 1107
Cfr10I RCCGGY 1 cut(s) 461
CseI GACGC 3 cut(s) 16, 1270, 1491
CsiI ACCWGGT 1 cut(s) 778
Csp6I GTAC 4 cut(s) 396, 1304, 1429, 1952
CspAI ACCGGT 1 cut(s) 461
CspCI CAANNNNNGTGG 2 cut(s) 2232, 2267
CviAII CATG 8 cut(s) 115, 266, 469, 718, 788, 953, 992, 1907
CviQI GTAC 4 cut(s) 396, 1304, 1429, 1952
DdeI CTNAG 2 cut(s) 648, 1208
DpnI GATC 9 cut(s) 80, 581, 699, 901, 983, 1413, 1479, 1989, 2119
DpnII GATC 9 cut(s) 78, 579, 697, 899, 981, 1411, 1477, 1987, 2117
DrdI GACNNNNNNGTC 1 cut(s) 59
DseDI GACNNNNNNGTC 1 cut(s) 59
Eam1104I CTCTTC 1 cut(s) 182
EarI CTCTTC 1 cut(s) 182
Eco130I CCWWGG 4 cut(s) 114, 991, 1614, 2283
Eco147I AGGCCT 1 cut(s) 1215
Eco24I GRGCYC 1 cut(s) 243
Eco31I GGTCTC 2 cut(s) 205, 452
Eco57I CTGAAG 4 cut(s) 144, 663, 1923, 2174
Eco88I CYCGRG 1 cut(s) 95
EcoRII CCWGG 3 cut(s) 181, 439, 778
EcoT14I CCWWGG 4 cut(s) 114, 991, 1614, 2283
EcoT22I ATGCAT 2 cut(s) 789, 1908
EcoT38I GRGCYC 1 cut(s) 243
ErhI CCWWGG 4 cut(s) 114, 991, 1614, 2283
Esp3I CGTCTC 1 cut(s) 1253
FaeI CATG 8 cut(s) 118, 269, 472, 721, 791, 956, 995, 1910
FalI AAGNNNNNCTT 2 cut(s) 2267, 2299
FaqI GGGAC 1 cut(s) 1781
FatI CATG 8 cut(s) 114, 265, 468, 717, 787, 952, 991, 1906
FauNDI CATATG 1 cut(s) 1818
FbaI TGATCA 2 cut(s) 981, 1411
FblI GTMKAC 4 cut(s) 655, 1745, 2203, 2289
Fnu4HI GCNGC 6 cut(s) 373, 621, 1145, 1176, 1346, 2268
FokI GGATG 5 cut(s) 356, 1210, 1268, 1793, 1949
FriOI GRGCYC 1 cut(s) 243
Fsp4HI GCNGC 6 cut(s) 373, 621, 1145, 1176, 1346, 2268
FspAI RTGCGCAY 1 cut(s) 944
FspBI CTAG 6 cut(s) 624, 1050, 1073, 1638, 2124, 2228
FspI TGCGCA 1 cut(s) 944
GlaI GCGC 3 cut(s) 858, 944, 1106
GluI GCNGC 6 cut(s) 373, 621, 1145, 1176, 1346, 2268
GsaI CCCAGC 1 cut(s) 1134
GsuI CTGGAG 6 cut(s) 29, 474, 983, 1151, 1517, 2318
HaeIII GGCC 1 cut(s) 1215
HapII CCGG 1 cut(s) 462
HgaI GACGC 3 cut(s) 16, 1270, 1491
HhaI GCGC 3 cut(s) 859, 945, 1107
Hin1I GRCGYC 1 cut(s) 1502
Hin1II CATG 8 cut(s) 118, 269, 472, 721, 791, 956, 995, 1910
Hin6I GCGC 3 cut(s) 857, 943, 1105
HinP1I GCGC 3 cut(s) 857, 943, 1105
HincII GTYRAC 2 cut(s) 1078, 1814
HindII GTYRAC 2 cut(s) 1078, 1814
HindIII AAGCTT 1 cut(s) 864
HpaI GTTAAC 1 cut(s) 1078
HpaII CCGG 1 cut(s) 462
HphI GGTGA 2 cut(s) 1100, 2072
Hpy99I CGWCG 2 cut(s) 14, 1507
HpyAV CCTTC 6 cut(s) 94, 1737, 1870, 2105, 2149, 2185
HpyCH4III ACNGT 8 cut(s) 256, 313, 395, 1317, 1428, 1543, 1956, 1982
HpyCH4IV ACGT 3 cut(s) 197, 520, 1607
HpyF10VI GCNNNNNNNGC 6 cut(s) 798, 863, 1104, 1242, 1508, 2228
HpyF3I CTNAG 2 cut(s) 648, 1208
HpySE526I ACGT 3 cut(s) 197, 520, 1607
Hsp92I GRCGYC 1 cut(s) 1502
Hsp92II CATG 8 cut(s) 118, 269, 472, 721, 791, 956, 995, 1910
HspAI GCGC 3 cut(s) 857, 943, 1105
Ksp22I TGATCA 2 cut(s) 981, 1411
KspAI GTTAAC 1 cut(s) 1078
Kzo9I GATC 9 cut(s) 78, 579, 697, 899, 981, 1411, 1477, 1987, 2117
LguI GCTCTTC 1 cut(s) 182
LmnI GCTCC 3 cut(s) 48, 294, 2227
Lsp1109I GCAGC 6 cut(s) 384, 607, 1156, 1162, 1357, 2254
LweI GCATC 8 cut(s) 925, 1254, 1650, 1745, 1771, 1971, 2053, 2155
MabI ACCWGGT 1 cut(s) 778
MaeI CTAG 6 cut(s) 624, 1050, 1073, 1638, 2124, 2228
MaeII ACGT 3 cut(s) 197, 520, 1607
MaeIII GTNAC 3 cut(s) 28, 118, 1389
MalI GATC 9 cut(s) 80, 581, 699, 901, 983, 1413, 1479, 1989, 2119
MboI GATC 9 cut(s) 78, 579, 697, 899, 981, 1411, 1477, 1987, 2117
MfeI CAATTG 2 cut(s) 154, 1366
MflI RGATCY 5 cut(s) 579, 697, 1477, 1987, 2117
MhlI GDGCHC 3 cut(s) 243, 1234, 1596
MluI ACGCGT 1 cut(s) 25
MlyI GAGTC 8 cut(s) 122, 990, 1036, 1643, 1801, 1865, 1904, 2153
MmeI TCCRAC 3 cut(s) 188, 1485, 1510
Mph1103I ATGCAT 2 cut(s) 789, 1908
MroXI GAANNNNTTC 2 cut(s) 1361, 1563
MspCI CTTAAG 1 cut(s) 632
MspI CCGG 1 cut(s) 462
MspR9I CCNGG 3 cut(s) 183, 441, 780
MunI CAATTG 2 cut(s) 154, 1366
Mva1269I GAATGC 3 cut(s) 441, 670, 1190
MvaI CCWGG 3 cut(s) 183, 441, 780
MvnI CGCG 5 cut(s) 27, 83, 233, 433, 1107
MwoI GCNNNNNNNGC 6 cut(s) 798, 863, 1104, 1242, 1508, 2228
NcoI CCATGG 2 cut(s) 114, 991
NdeI CATATG 1 cut(s) 1818
NdeII GATC 9 cut(s) 78, 579, 697, 899, 981, 1411, 1477, 1987, 2117
NlaIII CATG 8 cut(s) 118, 269, 472, 721, 791, 956, 995, 1910
NmuCI GTSAC 1 cut(s) 28
NsbI TGCGCA 1 cut(s) 944
NsiI ATGCAT 2 cut(s) 789, 1908
NspV TTCGAA 1 cut(s) 1355
PagI TCATGA 1 cut(s) 952
PaqCI CACCTGC 1 cut(s) 1605
PasI CCCWGGG 1 cut(s) 182
PceI AGGCCT 1 cut(s) 1215
PciSI GCTCTTC 1 cut(s) 182
PctI GAATGC 3 cut(s) 441, 670, 1190
PdmI GAANNNNTTC 2 cut(s) 1361, 1563
PfeI GAWTC 7 cut(s) 91, 485, 555, 752, 965, 1700, 2324
PinAI ACCGGT 1 cut(s) 461
PkrI GCNGC 6 cut(s) 374, 622, 1146, 1177, 1347, 2269
PleI GAGTC 8 cut(s) 122, 990, 1035, 1642, 1801, 1864, 1904, 2153
PpsI GAGTC 8 cut(s) 122, 990, 1035, 1642, 1801, 1864, 1904, 2153
PshAI GACNNNNGTC 1 cut(s) 1000
PshBI ATTAAT 1 cut(s) 734
PsiI TTATAA 1 cut(s) 1151
Psp1406I AACGTT 1 cut(s) 520
Psp6I CCWGG 3 cut(s) 181, 439, 778
PspFI CCCAGC 1 cut(s) 1130
PspGI CCWGG 3 cut(s) 181, 439, 778
PstI CTGCAG 4 cut(s) 539, 1146, 1180, 1602
PstNI CAGNNNCTG 1 cut(s) 1495
PsuI RGATCY 5 cut(s) 579, 697, 1477, 1987, 2117
RsaI GTAC 4 cut(s) 397, 1305, 1430, 1953
RsaNI GTAC 4 cut(s) 396, 1304, 1429, 1952
SapI GCTCTTC 1 cut(s) 182
SatI GCNGC 6 cut(s) 373, 621, 1145, 1176, 1346, 2268
Sau3AI GATC 9 cut(s) 78, 579, 697, 899, 981, 1411, 1477, 1987, 2117
SchI GAGTC 8 cut(s) 122, 990, 1036, 1643, 1801, 1865, 1904, 2153
ScrFI CCNGG 3 cut(s) 183, 441, 780
SduI GDGCHC 3 cut(s) 243, 1234, 1596
SexAI ACCWGGT 1 cut(s) 778
SfaNI GCATC 8 cut(s) 925, 1254, 1650, 1745, 1771, 1971, 2053, 2155
SfcI CTRYAG 4 cut(s) 535, 1142, 1176, 1598
SfuI TTCGAA 1 cut(s) 1355
SmlI CTYRAG 2 cut(s) 632, 1912
SmoI CTYRAG 2 cut(s) 632, 1912
SpeI ACTAGT 2 cut(s) 1049, 2123
SseBI AGGCCT 1 cut(s) 1215
SsiI CCGC 5 cut(s) 83, 148, 233, 431, 1038
SspMI CTAG 6 cut(s) 624, 1050, 1073, 1638, 2124, 2228
StuI AGGCCT 1 cut(s) 1215
StyD4I CCNGG 3 cut(s) 181, 439, 778
StyI CCWWGG 4 cut(s) 114, 991, 1614, 2283
TaaI ACNGT 8 cut(s) 256, 313, 395, 1317, 1428, 1543, 1956, 1982
TaiI ACGT 3 cut(s) 200, 523, 1610
TaqI TCGA 6 cut(s) 51, 243, 493, 898, 1355, 1524
TatI WGTACW 3 cut(s) 1303, 1428, 1951
TfiI GAWTC 7 cut(s) 91, 485, 555, 752, 965, 1700, 2324
TscAI CASTG 4 cut(s) 544, 1414, 1548, 1725
TseFI GTSAC 1 cut(s) 28
TseI GCWGC 6 cut(s) 372, 620, 1144, 1175, 1345, 2267
Tsp45I GTSAC 1 cut(s) 28
TspGWI ACGGA 2 cut(s) 24, 1783
TspRI CASTG 4 cut(s) 544, 1414, 1548, 1725
Vha464I CTTAAG 1 cut(s) 632
VspI ATTAAT 1 cut(s) 734
XapI RAATTY 2 cut(s) 505, 1619
XmiI GTMKAC 4 cut(s) 655, 1745, 2203, 2289
XmnI GAANNNNTTC 2 cut(s) 1361, 1563
XspI CTAG 6 cut(s) 624, 1050, 1073, 1638, 2124, 2228
Zsp2I ATGCAT 2 cut(s) 789, 1908
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.