Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5C

Orthogroup ID

OG0007288

Physical Location & Seq

Genomic Coordinates Forward (+)

4196169 .. 4202346

Gene Structure 6178 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5CG057000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2220 bp

ATGGTAACTGAAGACTCCAAGATTTTACTGCACCGCTTCAATTGGCAGAGGCTCTGGACAATCTCCCCAGGGAAGAGCATAACGTCTTTGTGTTGGAGACCTGATGGTAAAGCCATCGCCGTCGGGCTCGAAGATGGAACAGTCTCATTGCATGATGTTGAAAATGGAAAGTTGTTGAGGAGCTTGAAATCACATACTGTGGCTGTTGTTTCTCTTAATTGGGAGGAGGATGGACAGATGACCAGAGATGAGTATGGCAGCCACTCGGTATATGAAGACCGTACTTCTCGTTTCTTTCCACCACCTCCAAGAGTTCCGCGAATGCCTGGACTGGTATCTGGAGAGACCGGTTTCATGGACGATAGTGAAGATTCATTTCAAGAGCTGTCAAATTCTTCACAGCAACGTTTTAATATCCTCTGCAGTGGAGATAAAGATGGATTCATTTGCTTTAGTATATTTGGGATCTTTCCGATAGGGAAAATTAACATACACAACTTTGGAGCTGCTAGTCCACTTAAGGATACTGAAGCTGAGTGTAGACTTCTGAATGCTTCTGTTTACAAGGTTGCTTTGTCAAAAGATCTTTGTCATTTGATAGTCATGTGTTCAGGAGAATTAATTGAAGATAGGGAAGAATCAGAAAACAGGCAGATGACTGAACCTGGTATGCATGGCTTACATTGCATAGTGCTTGATACATCTATATTTTGGACGAGAAAAAGTGAGCTTCATCAAGTGGCGCAACAAGCTTCTAACATTGAGGAATTGGCAGAGGTTATTCGGTCATCAATTTCAGTTATGCACAAGGAGTGGTCTGATGCTATGCGCACTTTTCATGACAAGTTTGATTCTCTGTCCAATCTGATCATAGACCATGGACTCCAGTCCTCTCCCCAAGAGGAGTTTTTGAGTCTTTTAGGCGGTGCAAGGACTAGTCCAGCAGTTCATCAGTTTCTAGTTAACTCTCTCGGTGAAGTGGGCGTGAAGCGCGTGTCAAAGGCTGTTTGTGGTGCTGGGAAAGAACTGCAGCTTATAATCCTAAATCATCTCCAGCCTGCTGCAGAAATTATCGCATTCAGGATGGGAGAACTAAGAGGCCTTTCAAGATGGAGAGCACGCTATCAGGGCATCGGTTTGGATGAGACACTTATCAATAATGCAACAGAAAAAGCTGGAATGATACTTGTACAAGTTGAACGGTTTATCAGGGTGCTTTCATCTGTGGTGCAGCAGTTTTCGAACTTCTTCAATTGGCTCTTGAAGTGTATAAAGTTACTCAATTCAGAACCCAGTGATCATCATCTTCTACTGTACAATAGTGAACTTGTTGTTATATTCTTGAAGTTCCTATATGACCAAGATCCTGTTAAGCAGTTGCTGGAGGCGTCGGAAGCTGATGAGAGTATCGAAGTTGGATTGGAAACAGTGCAGAGAGTCAGAGAATTAGTTCAGTTTGGGGGATTTTCAGATTGTGAGCACCTGCAGAGGACGTTAGCCAAGGAATTTCAGCAGATGGAGTCTAGTTTTAAGGAGGCTTTTCAGATGCCCTTTATTACAATTTCAAGAAAGATAGTCTGTGAAGATTCATTGCCACTTTGCCCACTGCCACCTTCGTCAGCATCTTTGTCTACCAGTATTCCTATGTCAGTATCATATTATGAGGATGCCTCCCATTCCGTCCCTCATCAGACTCGTCAACATATGTTTCTTGACTACATATCTTTCCATATACCAGATGAGTCCTTCTCTGGTATGGAAAATTGTATAGGCATAGTGAGGGGGATTATGCATGACTCAAGCATTGTAAAGAGGGGTTATACTTCAGTAGAAGCTGTACTGTTGTGCATCCCTGTTGGTTATTACTGTGTGGATCTTTCTTTATATAAGGAAAGTCAGATTGTTTTATTATTAAATGAAACAACTACCACTTCTGAAAGCTCTGGTGATGCCTGTATGATGGTTGTGCAAGCAAATGACCTTCCATTTGTATCTATCACAAGATCCACTAGTCTAACCTACTGGAACTTGCATCAACTGAAGGACTCTGTTGTTCACTTGCAAATGGAGAACGAGAAGGTTCGCAGTATACCCCATTCTGTTATTGCTCCTCTAGCAGTTAGCTCATCAAGAGGTGTGGCTTGTGTTTTTGCTGCAAGAAAGCGTGCCTTGGTCTACATTCTGGAGGAAGATGAAGATGAAGTTTCTGATTCAGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0007275 GO:0008150 GO:0009790 GO:0009791 GO:0009793 GO:0009908 GO:0010154 GO:0022414 GO:0032501 GO:0032502 GO:0035670 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0048316 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048467 GO:0048481 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0061458 GO:0090567 GO:0099402

KEGG Pathways

Metabolic & Signaling

ko04110 ko04111 ko04113 ko04114 ko04120 ko04914 ko05166 map04110 map04111 map04113 map04114 map04120 map04914 map05166

Pfam Domains

Protein Families

ANAPC4 ANAPC4_WD40

Protein Analysis

739

Amino Acids

82.95

Weight (kDa)

5.27

Isoelectric Point (pI)

49.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ANAPC4_WD40	PF12894	1 - 76	1.7e-22	Anaphase-promoting complex subunit 4 WD40 domain
WD40_CDC20-Fz	PF24807	17 - 94	4.7e-10	CDC20/Fizzy WD40 domain
Beta-prop_THOC3	PF25174	19 - 80	7.1e-08	THOC3 beta-propeller domain
Beta-prop_EML_2	PF23414	24 - 81	1.7e-06	Echinoderm microtubule-associated protein second beta-propeller
WD40_Prp19	PF24814	24 - 78	2.1e-06	Prp19 WD40 domain
Beta-prop_WDR3_1st	PF25173	25 - 80	3.1e-08	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	25 - 80	6.6e-07	WDR5 beta-propeller domain
ANAPC4	PF12896	228 - 430	4.5e-57	Anaphase-promoting complex, cyclosome, subunit 4

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007288)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G21530
fragaria_vesca	FvH4_3g04050 FvH4_3g04050 FvH4_3g04050
malus_domestica	MD10G1303000.v1.1
prunus_persica	Prupe.4G036500_v2.0.a1 Prupe.4G036500_v2.0.a1 Prupe.4G036500_v2.0.a1 Prupe.4G036500_v2.0.a1 Prupe.4G036500_v2.0.a1 Prupe.4G036500_v2.0.a1
pyrus_communis	pycom10g25560
rosa_chinensis	RchiOBHm_Chr5g0006531
rosa_laevigata	RLG00000031396 RLG00000034652
rosa_multiflora	Rmu_sc0008991.1_g000016
rosa_roxburghii	Rroxscaffold_1G00031790 Rroxscaffold_1G00069530
rosa_rugosa	Rorug04G0421700
rosa_samantha	Rh5AG049400 Rh5BG048700 Rh5CG057000 Rh5DG048000
rosa_wichuraiana	Rw5G004950 Rw5G030980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1037
AarI	CACCTGC	1 cut(s)	1491
Acc16I	TGCGCA	1 cut(s)	830
Acc36I	ACCTGC	1 cut(s)	1491
AccI	GTMKAC	4 cut(s)	541, 1631, 2089, 2175
AccII	CGCG	2 cut(s)	319, 993
AciI	CCGC	3 cut(s)	34, 317, 924
AclI	AACGTT	1 cut(s)	406
AclWI	GGATC	4 cut(s)	473, 1358, 1881, 1998
AcsI	RAATTY	2 cut(s)	391, 1505
AcuI	CTGAAG	4 cut(s)	30, 549, 1809, 2060
AcyI	GRCGYC	1 cut(s)	1388
AfaI	GTAC	4 cut(s)	283, 1191, 1316, 1839
AflII	CTTAAG	1 cut(s)	518
AgeI	ACCGGT	1 cut(s)	347
AhlI	ACTAGT	2 cut(s)	935, 2009
AjnI	CCWGG	3 cut(s)	67, 325, 664
Alw21I	GWGCWC	2 cut(s)	1120, 1482
Alw26I	GTCTC	4 cut(s)	91, 148, 338, 1139
AlwI	GGATC	4 cut(s)	473, 1358, 1881, 1998
AlwNI	CAGNNNCTG	1 cut(s)	1381
AoxI	GGCC	1 cut(s)	1099
ApeKI	GCWGC	6 cut(s)	258, 506, 1030, 1061, 1231, 2153
ApoI	RAATTY	2 cut(s)	391, 1505
ArsI	GACNNNNNNTTYG	2 cut(s)	1970, 2002
AseI	ATTAAT	1 cut(s)	620
AsiGI	ACCGGT	1 cut(s)	347
Asp700I	GAANNNNTTC	2 cut(s)	1247, 1449
AspLEI	GCGC	3 cut(s)	745, 831, 993
AsuHPI	GGTGA	2 cut(s)	986, 1958
AsuII	TTCGAA	1 cut(s)	1241
BanII	GRGCYC	1 cut(s)	129
BbsI	GAAGAC	2 cut(s)	18, 282
Bbv12I	GWGCWC	2 cut(s)	1120, 1482
BbvI	GCAGC	6 cut(s)	270, 493, 1042, 1048, 1243, 2140
BccI	CCATC	9 cut(s)	98, 122, 128, 224, 431, 1078, 1104, 1510, 1954
BceAI	ACGGC	1 cut(s)	104
BciT130I	CCWGG	3 cut(s)	69, 327, 666
BciVI	GTATCC	1 cut(s)	517
BclI	TGATCA	2 cut(s)	867, 1297
BcoDI	GTCTC	4 cut(s)	91, 148, 338, 1139
BcuI	ACTAGT	2 cut(s)	935, 2009
BfaI	CTAG	6 cut(s)	510, 936, 959, 1524, 2010, 2114
BfmI	CTRYAG	4 cut(s)	421, 1028, 1062, 1484
BfrI	CTTAAG	1 cut(s)	518
BfuAI	ACCTGC	1 cut(s)	1491
BfuI	GTATCC	1 cut(s)	517
BglII	AGATCT	1 cut(s)	583
BisI	GCNGC	6 cut(s)	259, 507, 1031, 1062, 1232, 2154
BlsI	GCNGC	6 cut(s)	260, 508, 1032, 1063, 1233, 2155
Bme1390I	CCNGG	3 cut(s)	69, 327, 666
BmrFI	CCNGG	3 cut(s)	69, 327, 666
BmrI	ACTGGG	1 cut(s)	1287
BmsI	GCATC	8 cut(s)	811, 1140, 1536, 1631, 1657, 1857, 1939, 2041
BmuI	ACTGGG	1 cut(s)	1287
BoxI	GACNNNNGTC	1 cut(s)	886
BpiI	GAAGAC	2 cut(s)	18, 282
BplI	GAGNNNNNCTC	4 cut(s)	1655, 1687, 1733, 1765
BpmI	CTGGAG	5 cut(s)	360, 869, 1037, 1403, 2204
Bpu14I	TTCGAA	1 cut(s)	1241
BpuEI	CTTGAG	1 cut(s)	1783
BsaBI	GATNNNNATC	1 cut(s)	1302
BsaHI	GRCGYC	1 cut(s)	1388
BsaI	GGTCTC	2 cut(s)	91, 338
BsaJI	CCNNGG	5 cut(s)	67, 68, 877, 1500, 2169
BsaWI	WCCGGW	1 cut(s)	347
Bse118I	RCCGGY	1 cut(s)	347
Bse1I	ACTGG	5 cut(s)	336, 886, 1293, 1635, 2027
Bse3DI	GCAATG	3 cut(s)	146, 682, 1589
Bse8I	GATNNNNATC	1 cut(s)	1302
BseBI	CCWGG	3 cut(s)	69, 327, 666
BseDI	CCNNGG	5 cut(s)	67, 68, 877, 1500, 2169
BseGI	GGATG	5 cut(s)	235, 1089, 1147, 1672, 1848
BseJI	GATNNNNATC	1 cut(s)	1302
BseMI	GCAATG	3 cut(s)	146, 682, 1589
BseMII	CTCAG	1 cut(s)	525
BseNI	ACTGG	5 cut(s)	336, 886, 1293, 1635, 2027
BseRI	GAGGAG	4 cut(s)	193, 239, 917, 2100
BseXI	GCAGC	6 cut(s)	270, 493, 1042, 1048, 1243, 2140
BseYI	CCCAGC	1 cut(s)	1016
BsgI	GTGCAG	3 cut(s)	14, 1250, 1451
Bsh1236I	CGCG	2 cut(s)	319, 993
BshFI	GGCC	1 cut(s)	1101
BshTI	ACCGGT	1 cut(s)	347
BsiHKAI	GWGCWC	2 cut(s)	1120, 1482
BsiSI	CCGG	1 cut(s)	348
BslFI	GGGAC	1 cut(s)	1667
BsmAI	GTCTC	4 cut(s)	91, 148, 338, 1139
BsmFI	GGGAC	1 cut(s)	1667
BsmI	GAATGC	3 cut(s)	327, 556, 1076
BsnI	GGCC	1 cut(s)	1101
Bso31I	GGTCTC	2 cut(s)	91, 338
Bsp119I	TTCGAA	1 cut(s)	1241
Bsp1286I	GDGCHC	3 cut(s)	129, 1120, 1482
Bsp1407I	TGTACA	2 cut(s)	1189, 1314
Bsp143I	GATC	7 cut(s)	465, 583, 867, 1297, 1363, 1873, 2003
Bsp19I	CCATGG	1 cut(s)	877
BspACI	CCGC	3 cut(s)	34, 317, 924
BspANI	GGCC	1 cut(s)	1101
BspCNI	CTCAG	1 cut(s)	526
BspFNI	CGCG	2 cut(s)	319, 993
BspHI	TCATGA	1 cut(s)	838
BspMAI	CTGCAG	4 cut(s)	425, 1032, 1066, 1488
BspMI	ACCTGC	1 cut(s)	1491
BspPI	GGATC	4 cut(s)	473, 1358, 1881, 1998
BspQI	GCTCTTC	1 cut(s)	68
BspT104I	TTCGAA	1 cut(s)	1241
BspTI	CTTAAG	1 cut(s)	518
BspTNI	GGTCTC	2 cut(s)	91, 338
BsrDI	GCAATG	3 cut(s)	146, 682, 1589
BsrFI	RCCGGY	1 cut(s)	347
BsrGI	TGTACA	2 cut(s)	1189, 1314
BsrI	ACTGG	5 cut(s)	336, 886, 1293, 1635, 2027
BssAI	RCCGGY	1 cut(s)	347
BssECI	CCNNGG	5 cut(s)	67, 68, 877, 1500, 2169
BssMI	GATC	7 cut(s)	465, 583, 867, 1297, 1363, 1873, 2003
BssNAI	GTATAC	1 cut(s)	2090
BssNI	GRCGYC	1 cut(s)	1388
BssT1I	CCWWGG	3 cut(s)	877, 1500, 2169
Bst1107I	GTATAC	1 cut(s)	2090
Bst2UI	CCWGG	3 cut(s)	69, 327, 666
Bst4CI	ACNGT	8 cut(s)	142, 199, 281, 1203, 1314, 1429, 1842, 1868
Bst6I	CTCTTC	1 cut(s)	68
BstACI	GRCGYC	1 cut(s)	1388
BstAFI	CTTAAG	1 cut(s)	518
BstAUI	TGTACA	2 cut(s)	1189, 1314
BstBI	TTCGAA	1 cut(s)	1241
BstC8I	GCNNGC	4 cut(s)	1059, 1120, 1971, 2166
BstDEI	CTNAG	2 cut(s)	534, 1094
BstDSI	CCRYGG	1 cut(s)	877
BstF5I	GGATG	5 cut(s)	235, 1089, 1147, 1672, 1848
BstFNI	CGCG	2 cut(s)	319, 993
BstHHI	GCGC	3 cut(s)	745, 831, 993
BstKTI	GATC	7 cut(s)	468, 586, 870, 1300, 1366, 1876, 2006
BstMAI	GTCTC	4 cut(s)	91, 148, 338, 1139
BstMBI	GATC	7 cut(s)	465, 583, 867, 1297, 1363, 1873, 2003
BstMWI	GCNNNNNNNGC	6 cut(s)	684, 749, 990, 1128, 1394, 2114
BstNI	CCWGG	3 cut(s)	69, 327, 666
BstPAI	GACNNNNGTC	1 cut(s)	886
BstSCI	CCNGG	3 cut(s)	67, 325, 664
BstSFI	CTRYAG	4 cut(s)	421, 1028, 1062, 1484
BstUI	CGCG	2 cut(s)	319, 993
BstV1I	GCAGC	6 cut(s)	270, 493, 1042, 1048, 1243, 2140
BstV2I	GAAGAC	2 cut(s)	18, 282
BstX2I	RGATCY	5 cut(s)	465, 583, 1363, 1873, 2003
BstYI	RGATCY	5 cut(s)	465, 583, 1363, 1873, 2003
BstZ17I	GTATAC	1 cut(s)	2090
BsuI	GTATCC	1 cut(s)	517
BsuRI	GGCC	1 cut(s)	1101
BtgI	CCRYGG	1 cut(s)	877
BtgZI	GCGATG	1 cut(s)	100
BtsCI	GGATG	5 cut(s)	235, 1089, 1147, 1672, 1848
BtsI	GCAGTG	2 cut(s)	430, 1604
BtsIMutI	CAGTG	4 cut(s)	430, 1300, 1434, 1604
BveI	ACCTGC	1 cut(s)	1491
Cac8I	GCNNGC	4 cut(s)	1059, 1120, 1971, 2166
CaiI	CAGNNNCTG	1 cut(s)	1381
CciI	TCATGA	1 cut(s)	838
CfoI	GCGC	3 cut(s)	745, 831, 993
Cfr10I	RCCGGY	1 cut(s)	347
CseI	GACGC	1 cut(s)	1377
CsiI	ACCWGGT	1 cut(s)	664
Csp6I	GTAC	4 cut(s)	282, 1190, 1315, 1838
CspAI	ACCGGT	1 cut(s)	347
CspCI	CAANNNNNGTGG	2 cut(s)	2118, 2153
CviAII	CATG	7 cut(s)	152, 355, 604, 674, 839, 878, 1793
CviQI	GTAC	4 cut(s)	282, 1190, 1315, 1838
DdeI	CTNAG	2 cut(s)	534, 1094
DpnI	GATC	7 cut(s)	467, 585, 869, 1299, 1365, 1875, 2005
DpnII	GATC	7 cut(s)	465, 583, 867, 1297, 1363, 1873, 2003
Eam1104I	CTCTTC	1 cut(s)	68
EarI	CTCTTC	1 cut(s)	68
Eco130I	CCWWGG	3 cut(s)	877, 1500, 2169
Eco147I	AGGCCT	1 cut(s)	1101
Eco24I	GRGCYC	1 cut(s)	129
Eco31I	GGTCTC	2 cut(s)	91, 338
Eco57I	CTGAAG	4 cut(s)	30, 549, 1809, 2060
EcoRII	CCWGG	3 cut(s)	67, 325, 664
EcoT14I	CCWWGG	3 cut(s)	877, 1500, 2169
EcoT22I	ATGCAT	2 cut(s)	675, 1794
EcoT38I	GRGCYC	1 cut(s)	129
ErhI	CCWWGG	3 cut(s)	877, 1500, 2169
FaeI	CATG	7 cut(s)	155, 358, 607, 677, 842, 881, 1796
FalI	AAGNNNNNCTT	2 cut(s)	2153, 2185
FaqI	GGGAC	1 cut(s)	1667
FatI	CATG	7 cut(s)	151, 354, 603, 673, 838, 877, 1792
FauNDI	CATATG	1 cut(s)	1704
FbaI	TGATCA	2 cut(s)	867, 1297
FblI	GTMKAC	4 cut(s)	541, 1631, 2089, 2175
Fnu4HI	GCNGC	6 cut(s)	259, 507, 1031, 1062, 1232, 2154
FokI	GGATG	5 cut(s)	242, 1096, 1154, 1679, 1835
FriOI	GRGCYC	1 cut(s)	129
Fsp4HI	GCNGC	6 cut(s)	259, 507, 1031, 1062, 1232, 2154
FspAI	RTGCGCAY	1 cut(s)	830
FspBI	CTAG	6 cut(s)	510, 936, 959, 1524, 2010, 2114
FspI	TGCGCA	1 cut(s)	830
GlaI	GCGC	3 cut(s)	744, 830, 992
GluI	GCNGC	6 cut(s)	259, 507, 1031, 1062, 1232, 2154
GsaI	CCCAGC	1 cut(s)	1020
GsuI	CTGGAG	5 cut(s)	360, 869, 1037, 1403, 2204
HaeIII	GGCC	1 cut(s)	1101
HapII	CCGG	1 cut(s)	348
HgaI	GACGC	1 cut(s)	1377
HhaI	GCGC	3 cut(s)	745, 831, 993
Hin1I	GRCGYC	1 cut(s)	1388
Hin1II	CATG	7 cut(s)	155, 358, 607, 677, 842, 881, 1796
Hin6I	GCGC	3 cut(s)	743, 829, 991
HinP1I	GCGC	3 cut(s)	743, 829, 991
HincII	GTYRAC	2 cut(s)	964, 1700
HindII	GTYRAC	2 cut(s)	964, 1700
HindIII	AAGCTT	1 cut(s)	750
HpaI	GTTAAC	1 cut(s)	964
HpaII	CCGG	1 cut(s)	348
HphI	GGTGA	2 cut(s)	986, 1958
Hpy99I	CGWCG	2 cut(s)	125, 1393
HpyAV	CCTTC	5 cut(s)	1623, 1756, 1991, 2035, 2071
HpyCH4III	ACNGT	8 cut(s)	142, 199, 281, 1203, 1314, 1429, 1842, 1868
HpyCH4IV	ACGT	3 cut(s)	83, 406, 1493
HpyF10VI	GCNNNNNNNGC	6 cut(s)	684, 749, 990, 1128, 1394, 2114
HpyF3I	CTNAG	2 cut(s)	534, 1094
HpySE526I	ACGT	3 cut(s)	83, 406, 1493
Hsp92I	GRCGYC	1 cut(s)	1388
Hsp92II	CATG	7 cut(s)	155, 358, 607, 677, 842, 881, 1796
HspAI	GCGC	3 cut(s)	743, 829, 991
Ksp22I	TGATCA	2 cut(s)	867, 1297
KspAI	GTTAAC	1 cut(s)	964
Kzo9I	GATC	7 cut(s)	465, 583, 867, 1297, 1363, 1873, 2003
LguI	GCTCTTC	1 cut(s)	68
LmnI	GCTCC	3 cut(s)	180, 503, 2113
Lsp1109I	GCAGC	6 cut(s)	270, 493, 1042, 1048, 1243, 2140
LweI	GCATC	8 cut(s)	811, 1140, 1536, 1631, 1657, 1857, 1939, 2041
MabI	ACCWGGT	1 cut(s)	664
MaeI	CTAG	6 cut(s)	510, 936, 959, 1524, 2010, 2114
MaeII	ACGT	3 cut(s)	83, 406, 1493
MaeIII	GTNAC	2 cut(s)	4, 1275
MalI	GATC	7 cut(s)	467, 585, 869, 1299, 1365, 1875, 2005
MboI	GATC	7 cut(s)	465, 583, 867, 1297, 1363, 1873, 2003
MfeI	CAATTG	2 cut(s)	40, 1252
MflI	RGATCY	5 cut(s)	465, 583, 1363, 1873, 2003
MhlI	GDGCHC	3 cut(s)	129, 1120, 1482
MlyI	GAGTC	9 cut(s)	8, 876, 922, 1446, 1529, 1687, 1751, 1790, 2039
MmeI	TCCRAC	3 cut(s)	74, 1371, 1396
Mph1103I	ATGCAT	2 cut(s)	675, 1794
MroXI	GAANNNNTTC	2 cut(s)	1247, 1449
MseI	TTAA	9 cut(s)	216, 411, 486, 519, 620, 963, 1371, 1530, 1913
MspCI	CTTAAG	1 cut(s)	518
MspI	CCGG	1 cut(s)	348
MspR9I	CCNGG	3 cut(s)	69, 327, 666
MunI	CAATTG	2 cut(s)	40, 1252
Mva1269I	GAATGC	3 cut(s)	327, 556, 1076
MvaI	CCWGG	3 cut(s)	69, 327, 666
MvnI	CGCG	2 cut(s)	319, 993
MwoI	GCNNNNNNNGC	6 cut(s)	684, 749, 990, 1128, 1394, 2114
NcoI	CCATGG	1 cut(s)	877
NdeI	CATATG	1 cut(s)	1704
NdeII	GATC	7 cut(s)	465, 583, 867, 1297, 1363, 1873, 2003
NlaIII	CATG	7 cut(s)	155, 358, 607, 677, 842, 881, 1796
NsbI	TGCGCA	1 cut(s)	830
NsiI	ATGCAT	2 cut(s)	675, 1794
NspV	TTCGAA	1 cut(s)	1241
PagI	TCATGA	1 cut(s)	838
PaqCI	CACCTGC	1 cut(s)	1491
PasI	CCCWGGG	1 cut(s)	68
PceI	AGGCCT	1 cut(s)	1101
PciSI	GCTCTTC	1 cut(s)	68
PctI	GAATGC	3 cut(s)	327, 556, 1076
PdmI	GAANNNNTTC	2 cut(s)	1247, 1449
PfeI	GAWTC	6 cut(s)	371, 441, 638, 851, 1586, 2210
PinAI	ACCGGT	1 cut(s)	347
PkrI	GCNGC	6 cut(s)	260, 508, 1032, 1063, 1233, 2155
PleI	GAGTC	9 cut(s)	8, 876, 921, 1445, 1528, 1687, 1750, 1790, 2039
PpsI	GAGTC	9 cut(s)	8, 876, 921, 1445, 1528, 1687, 1750, 1790, 2039
PshAI	GACNNNNGTC	1 cut(s)	886
PshBI	ATTAAT	1 cut(s)	620
PsiI	TTATAA	1 cut(s)	1037
Psp1406I	AACGTT	1 cut(s)	406
Psp6I	CCWGG	3 cut(s)	67, 325, 664
PspFI	CCCAGC	1 cut(s)	1016
PspGI	CCWGG	3 cut(s)	67, 325, 664
PstI	CTGCAG	4 cut(s)	425, 1032, 1066, 1488
PstNI	CAGNNNCTG	1 cut(s)	1381
PsuI	RGATCY	5 cut(s)	465, 583, 1363, 1873, 2003
RsaI	GTAC	4 cut(s)	283, 1191, 1316, 1839
RsaNI	GTAC	4 cut(s)	282, 1190, 1315, 1838
SapI	GCTCTTC	1 cut(s)	68
SaqAI	TTAA	9 cut(s)	216, 411, 486, 519, 620, 963, 1371, 1530, 1913
SatI	GCNGC	6 cut(s)	259, 507, 1031, 1062, 1232, 2154
Sau3AI	GATC	7 cut(s)	465, 583, 867, 1297, 1363, 1873, 2003
SchI	GAGTC	9 cut(s)	8, 876, 922, 1446, 1529, 1687, 1751, 1790, 2039
ScrFI	CCNGG	3 cut(s)	69, 327, 666
SduI	GDGCHC	3 cut(s)	129, 1120, 1482
SexAI	ACCWGGT	1 cut(s)	664
SfaNI	GCATC	8 cut(s)	811, 1140, 1536, 1631, 1657, 1857, 1939, 2041
SfcI	CTRYAG	4 cut(s)	421, 1028, 1062, 1484
SfuI	TTCGAA	1 cut(s)	1241
SmlI	CTYRAG	2 cut(s)	518, 1798
SmoI	CTYRAG	2 cut(s)	518, 1798
SpeI	ACTAGT	2 cut(s)	935, 2009
SseBI	AGGCCT	1 cut(s)	1101
SsiI	CCGC	3 cut(s)	34, 317, 924
SspMI	CTAG	6 cut(s)	510, 936, 959, 1524, 2010, 2114
StuI	AGGCCT	1 cut(s)	1101
StyD4I	CCNGG	3 cut(s)	67, 325, 664
StyI	CCWWGG	3 cut(s)	877, 1500, 2169
TaaI	ACNGT	8 cut(s)	142, 199, 281, 1203, 1314, 1429, 1842, 1868
TaiI	ACGT	3 cut(s)	86, 409, 1496
TaqI	TCGA	3 cut(s)	129, 1241, 1410
TaqII	GACCGA	1 cut(s)	774
TatI	WGTACW	3 cut(s)	1189, 1314, 1837
TfiI	GAWTC	6 cut(s)	371, 441, 638, 851, 1586, 2210
Tru1I	TTAA	9 cut(s)	216, 411, 486, 519, 620, 963, 1371, 1530, 1913
Tru9I	TTAA	9 cut(s)	216, 411, 486, 519, 620, 963, 1371, 1530, 1913
TscAI	CASTG	4 cut(s)	430, 1300, 1434, 1611
TseI	GCWGC	6 cut(s)	258, 506, 1030, 1061, 1231, 2153
TspGWI	ACGGA	1 cut(s)	1669
TspRI	CASTG	4 cut(s)	430, 1300, 1434, 1611
Vha464I	CTTAAG	1 cut(s)	518
VspI	ATTAAT	1 cut(s)	620
XapI	RAATTY	2 cut(s)	391, 1505
XmiI	GTMKAC	4 cut(s)	541, 1631, 2089, 2175
XmnI	GAANNNNTTC	2 cut(s)	1247, 1449
XspI	CTAG	6 cut(s)	510, 936, 959, 1524, 2010, 2114
Zsp2I	ATGCAT	2 cut(s)	675, 1794

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.