Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0007288

Physical Location & Seq

Genomic Coordinates Reverse (-)

47188347 .. 47192571

Gene Structure 4225 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000034652

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1305 bp

ATGGTAACTGAGGACTCCAAGATATTACTGCACCGCTTCAATTGGCAGAGGCTCGGGACGATCTCCCCAGGGAAGAGCATAACGTCTTTGTGTTGGAGACCTGATGGTAAAGCAATCGCCGTCGGGCTTGAAGATGGAACAGTCTCATTGCATGATGTTGAAAATGGAAAGTTGTTGAGGAGCTTGAAATCACATACTGTGGCTGTTGTTTCTCTTAATTGGGAGGAGGATGGACAGATGACTAGAGTATGTGCCAAATTGATTCTGTTGGCCACTCGGTATATGAAGACCGTACTTCTCATTTCTTTCCACCACCTCCAAGAGTTCCGCGAATGCCTGGACTGTGGAGATAAAGATGGATTCATTTGCTTTAGTATATTTGGGATCTTTCCGATAGGGAAAATTAACATACACAACTTTGGTGCTGCTAGTCCACTTAAGGATACTGAACCTGAGTGTAGACTTATGAATGCTTCTGCTTACAAGGTTGCTTTGTCAAAAGATCTTTGTCATTTGATAGTCGTGTGTTCAGGAGAATTAATTGAAGATACGGAAGAATCAGAAAACAGAAAAAGTGAGCTTCATCAAGTGGCGCAACAAGCTTCTAACATTGAGGAATTGGCAGAGGTTATTCGGTCATCAATTTCAGTTATGCACAAGGAGTGGTGTGATGCTATGCGCACTTTTCATGACAAGTTTGATTCTCTGTCTAATCTGATCATAGACCATGGAGTTTTGAGTCTTTTAGGCGGTGCAAGGACTAGTCCAGCAGTTCATCAGTTTCTAGTTAACTCTCTCGTTGAAGTGGGTGTGAAGCGCGTGTCAAAGGCTGTTTGTGGTGCTGGGAAAGAACTGCAGCTTATAATCCTAATCCATCTCCAGCCTGCTGCAGAAATTATTGCATTCAGGATGGGAGAACTAGGGGGCCTTTCACGATGGAGAGCATGCTATCAGGGCATCGGTTTGGATGAGTCGCTTATCAATAATGCAACAGAAAAAGCTGGAATGATACTTGTACAAGTTGAATGGTTTATCAGGGTGCTTTCATCTGTGGTGCAGCAGTTTTCAAACTTCTTCAATTGGCTCTTTAAGTGTATAAAGTTTCTCAATTCAGAATCCAGTGATCATCATCTTCTACTGTACAATAGTGAACTTGTTATATTCTTGAAGTTTTTATATGACCAAGATCCTGTTAAGCAGTTGCTGGAGGCGTCAGAAGCTGATGAGAGTATAGAAGTTGGATTATGGAGTCTAGGAGGCTTTCCAGATGCCCTTTACTACAATTTCAAGAAAGATAGTCTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0007275 GO:0008150 GO:0009790 GO:0009791 GO:0009793 GO:0009908 GO:0010154 GO:0022414 GO:0032501 GO:0032502 GO:0035670 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0048316 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048467 GO:0048481 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0061458 GO:0090567 GO:0099402

KEGG Pathways

Metabolic & Signaling

ko04110 ko04111 ko04113 ko04114 ko04120 ko04914 ko05166 map04110 map04111 map04113 map04114 map04120 map04914 map05166

Pfam Domains

Protein Families

ANAPC4 ANAPC4_WD40

Protein Analysis

435

Amino Acids

48.64

Weight (kDa)

5.7

Isoelectric Point (pI)

46.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ANAPC4_WD40	PF12894	1 - 76	2e-20	Anaphase-promoting complex subunit 4 WD40 domain
WD40_WDHD1_1st	PF24817	8 - 79	9.5e-06	WDHD1 first WD40 domain
Beta-prop_WDR36-Utp21_1st	PF25171	9 - 81	3.7e-06	WDR36/Utp21 first beta-propeller
Beta-prop_THOC3	PF25174	18 - 84	1.2e-08	THOC3 beta-propeller domain
WD40_CDC20-Fz	PF24807	22 - 78	9.3e-10	CDC20/Fizzy WD40 domain
WD40_Prp19	PF24814	24 - 78	8.2e-07	Prp19 WD40 domain
Beta-prop_WDR3_1st	PF25173	25 - 81	8.7e-09	WDR3 first beta-propeller domain
Beta-prop_EML_2	PF23414	25 - 85	4e-07	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5	PF25175	26 - 80	2.4e-07	WDR5 beta-propeller domain
ANAPC4	PF12896	183 - 372	9.2e-47	Anaphase-promoting complex, cyclosome, subunit 4

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0007288)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G21530
fragaria_vesca	FvH4_3g04050 FvH4_3g04050 FvH4_3g04050
malus_domestica	MD10G1303000.v1.1
prunus_persica	Prupe.4G036500_v2.0.a1 Prupe.4G036500_v2.0.a1 Prupe.4G036500_v2.0.a1 Prupe.4G036500_v2.0.a1 Prupe.4G036500_v2.0.a1 Prupe.4G036500_v2.0.a1
pyrus_communis	pycom10g25560
rosa_chinensis	RchiOBHm_Chr5g0006531
rosa_laevigata	RLG00000031396 RLG00000034652
rosa_multiflora	Rmu_sc0008991.1_g000016
rosa_roxburghii	Rroxscaffold_1G00031790 Rroxscaffold_1G00069530
rosa_rugosa	Rorug04G0421700
rosa_samantha	Rh5AG049400 Rh5BG048700 Rh5CG057000 Rh5DG048000
rosa_wichuraiana	Rw5G004950 Rw5G030980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	863
Acc16I	TGCGCA	1 cut(s)	680
AccI	GTMKAC	1 cut(s)	460
AccII	CGCG	2 cut(s)	330, 819
AciI	CCGC	3 cut(s)	34, 328, 750
AclWI	GGATC	2 cut(s)	392, 1181
AcoI	YGGCCR	1 cut(s)	270
AcyI	GRCGYC	1 cut(s)	1211
AfaI	GTAC	3 cut(s)	294, 1017, 1142
AflII	CTTAAG	1 cut(s)	437
AhlI	ACTAGT	1 cut(s)	761
AjnI	CCWGG	2 cut(s)	67, 336
AluBI	AGCT	6 cut(s)	183, 580, 602, 859, 1001, 1220
AluI	AGCT	6 cut(s)	183, 580, 602, 859, 1001, 1220
Alw26I	GTCTC	2 cut(s)	91, 148
AlwI	GGATC	2 cut(s)	392, 1181
AlwNI	CAGNNNCTG	2 cut(s)	1204, 1220
Ama87I	CYCGRG	1 cut(s)	53
AoxI	GGCC	2 cut(s)	270, 925
ApeKI	GCWGC	4 cut(s)	425, 856, 887, 1057
AseI	ATTAAT	1 cut(s)	539
AspLEI	GCGC	3 cut(s)	595, 681, 819
AspS9I	GGNCC	1 cut(s)	925
AvaI	CYCGRG	1 cut(s)	53
BalI	TGGCCA	1 cut(s)	272
BbsI	GAAGAC	1 cut(s)	293
BbvI	GCAGC	4 cut(s)	412, 868, 874, 1069
BccI	CCATC	7 cut(s)	98, 128, 224, 350, 882, 904, 930
BceAI	ACGGC	1 cut(s)	104
BciT130I	CCWGG	2 cut(s)	69, 338
BciVI	GTATCC	1 cut(s)	436
BclI	TGATCA	2 cut(s)	717, 1123
BcoDI	GTCTC	2 cut(s)	91, 148
BcuI	ACTAGT	1 cut(s)	761
BfaI	CTAG	6 cut(s)	243, 429, 762, 785, 920, 1253
BfmI	CTRYAG	2 cut(s)	854, 888
BfrI	CTTAAG	1 cut(s)	437
BfuI	GTATCC	1 cut(s)	436
BglII	AGATCT	1 cut(s)	502
BisI	GCNGC	4 cut(s)	426, 857, 888, 1058
BlsI	GCNGC	4 cut(s)	427, 858, 889, 1059
Bme1390I	CCNGG	2 cut(s)	69, 338
BmeT110I	CYCGRG	1 cut(s)	53
BmgT120I	GGNCC	1 cut(s)	925
BmiI	GGNNCC	1 cut(s)	926
BmrFI	CCNGG	2 cut(s)	69, 338
BmsI	GCATC	3 cut(s)	661, 966, 1258
BpiI	GAAGAC	1 cut(s)	293
BpmI	CTGGAG	2 cut(s)	863, 1226
BsaBI	GATNNNNATC	1 cut(s)	1128
BsaHI	GRCGYC	1 cut(s)	1211
BsaI	GGTCTC	1 cut(s)	91
BsaJI	CCNNGG	3 cut(s)	67, 68, 727
Bse1I	ACTGG	1 cut(s)	1119
Bse3DI	GCAATG	1 cut(s)	146
Bse8I	GATNNNNATC	1 cut(s)	1128
BseBI	CCWGG	2 cut(s)	69, 338
BseDI	CCNNGG	3 cut(s)	67, 68, 727
BseGI	GGATG	3 cut(s)	235, 915, 973
BseJI	GATNNNNATC	1 cut(s)	1128
BseMI	GCAATG	1 cut(s)	146
BseMII	CTCAG	1 cut(s)	444
BseNI	ACTGG	1 cut(s)	1119
BseRI	GAGGAG	2 cut(s)	193, 239
BseXI	GCAGC	4 cut(s)	412, 868, 874, 1069
BseYI	CCCAGC	1 cut(s)	842
BsgI	GTGCAG	2 cut(s)	14, 1076
Bsh1236I	CGCG	2 cut(s)	330, 819
BshFI	GGCC	2 cut(s)	272, 927
BsiHKCI	CYCGRG	1 cut(s)	53
BslFI	GGGAC	1 cut(s)	70
BsmAI	GTCTC	2 cut(s)	91, 148
BsmFI	GGGAC	1 cut(s)	70
BsmI	GAATGC	3 cut(s)	338, 475, 902
BsnI	GGCC	2 cut(s)	272, 927
Bso31I	GGTCTC	1 cut(s)	91
BsoBI	CYCGRG	1 cut(s)	53
Bsp1407I	TGTACA	2 cut(s)	1015, 1140
Bsp143I	GATC	6 cut(s)	60, 384, 502, 717, 1123, 1186
Bsp19I	CCATGG	1 cut(s)	727
BspACI	CCGC	3 cut(s)	34, 328, 750
BspANI	GGCC	2 cut(s)	272, 927
BspCNI	CTCAG	1 cut(s)	445
BspFNI	CGCG	2 cut(s)	330, 819
BspHI	TCATGA	1 cut(s)	688
BspLI	GGNNCC	1 cut(s)	926
BspMAI	CTGCAG	2 cut(s)	858, 892
BspPI	GGATC	2 cut(s)	392, 1181
BspQI	GCTCTTC	1 cut(s)	68
BspTI	CTTAAG	1 cut(s)	437
BspTNI	GGTCTC	1 cut(s)	91
BsrDI	GCAATG	1 cut(s)	146
BsrGI	TGTACA	2 cut(s)	1015, 1140
BsrI	ACTGG	1 cut(s)	1119
BssECI	CCNNGG	3 cut(s)	67, 68, 727
BssMI	GATC	6 cut(s)	60, 384, 502, 717, 1123, 1186
BssNI	GRCGYC	1 cut(s)	1211
BssT1I	CCWWGG	1 cut(s)	727
Bst2UI	CCWGG	2 cut(s)	69, 338
Bst4CI	ACNGT	5 cut(s)	142, 199, 292, 344, 1140
Bst6I	CTCTTC	1 cut(s)	68
BstACI	GRCGYC	1 cut(s)	1211
BstAFI	CTTAAG	1 cut(s)	437
BstAUI	TGTACA	2 cut(s)	1015, 1140
BstC8I	GCNNGC	2 cut(s)	885, 946
BstDEI	CTNAG	2 cut(s)	9, 453
BstDSI	CCRYGG	1 cut(s)	727
BstF5I	GGATG	3 cut(s)	235, 915, 973
BstFNI	CGCG	2 cut(s)	330, 819
BstHHI	GCGC	3 cut(s)	595, 681, 819
BstKTI	GATC	6 cut(s)	63, 387, 505, 720, 1126, 1189
BstMAI	GTCTC	2 cut(s)	91, 148
BstMBI	GATC	6 cut(s)	60, 384, 502, 717, 1123, 1186
BstMWI	GCNNNNNNNGC	3 cut(s)	599, 954, 1217
BstNI	CCWGG	2 cut(s)	69, 338
BstNSI	RCATGY	1 cut(s)	948
BstSCI	CCNGG	2 cut(s)	67, 336
BstSFI	CTRYAG	2 cut(s)	854, 888
BstUI	CGCG	2 cut(s)	330, 819
BstV1I	GCAGC	4 cut(s)	412, 868, 874, 1069
BstV2I	GAAGAC	1 cut(s)	293
BstX2I	RGATCY	3 cut(s)	384, 502, 1186
BstYI	RGATCY	3 cut(s)	384, 502, 1186
BsuI	GTATCC	1 cut(s)	436
BsuRI	GGCC	2 cut(s)	272, 927
BtgI	CCRYGG	1 cut(s)	727
BtsCI	GGATG	3 cut(s)	235, 915, 973
BtsIMutI	CAGTG	1 cut(s)	1126
Cac8I	GCNNGC	2 cut(s)	885, 946
CaiI	CAGNNNCTG	2 cut(s)	1204, 1220
CciI	TCATGA	1 cut(s)	688
CfoI	GCGC	3 cut(s)	595, 681, 819
Cfr13I	GGNCC	1 cut(s)	925
CseI	GACGC	1 cut(s)	1200
Csp6I	GTAC	3 cut(s)	293, 1016, 1141
CviAII	CATG	4 cut(s)	152, 689, 728, 945
CviQI	GTAC	3 cut(s)	293, 1016, 1141
DdeI	CTNAG	2 cut(s)	9, 453
DpnI	GATC	6 cut(s)	62, 386, 504, 719, 1125, 1188
DpnII	GATC	6 cut(s)	60, 384, 502, 717, 1123, 1186
EaeI	YGGCCR	1 cut(s)	270
Eam1104I	CTCTTC	1 cut(s)	68
EarI	CTCTTC	1 cut(s)	68
Eco130I	CCWWGG	1 cut(s)	727
Eco31I	GGTCTC	1 cut(s)	91
Eco88I	CYCGRG	1 cut(s)	53
EcoO109I	RGGNCCY	1 cut(s)	925
EcoRII	CCWGG	2 cut(s)	67, 336
EcoT14I	CCWWGG	1 cut(s)	727
ErhI	CCWWGG	1 cut(s)	727
FaeI	CATG	4 cut(s)	155, 692, 731, 948
FaqI	GGGAC	1 cut(s)	70
FatI	CATG	4 cut(s)	151, 688, 727, 944
FbaI	TGATCA	2 cut(s)	717, 1123
FblI	GTMKAC	1 cut(s)	460
Fnu4HI	GCNGC	4 cut(s)	426, 857, 888, 1058
FokI	GGATG	3 cut(s)	242, 922, 980
Fsp4HI	GCNGC	4 cut(s)	426, 857, 888, 1058
FspAI	RTGCGCAY	1 cut(s)	680
FspBI	CTAG	6 cut(s)	243, 429, 762, 785, 920, 1253
FspI	TGCGCA	1 cut(s)	680
GlaI	GCGC	3 cut(s)	594, 680, 818
GluI	GCNGC	4 cut(s)	426, 857, 888, 1058
GsaI	CCCAGC	1 cut(s)	846
GsuI	CTGGAG	2 cut(s)	863, 1226
HaeIII	GGCC	2 cut(s)	272, 927
HgaI	GACGC	1 cut(s)	1200
HhaI	GCGC	3 cut(s)	595, 681, 819
Hin1I	GRCGYC	1 cut(s)	1211
Hin1II	CATG	4 cut(s)	155, 692, 731, 948
Hin6I	GCGC	3 cut(s)	593, 679, 817
HinP1I	GCGC	3 cut(s)	593, 679, 817
HincII	GTYRAC	1 cut(s)	790
HindII	GTYRAC	1 cut(s)	790
HindIII	AAGCTT	1 cut(s)	600
HinfI	GANTC	9 cut(s)	14, 262, 360, 557, 701, 739, 971, 1115, 1249
HpaI	GTTAAC	1 cut(s)	790
Hpy166II	GTNNAC	4 cut(s)	434, 461, 790, 1151
Hpy188I	TCNGA	5 cut(s)	393, 562, 717, 1114, 1216
Hpy188III	TCNNGA	8 cut(s)	55, 531, 689, 907, 933, 1165, 1265, 1288
Hpy8I	GTNNAC	4 cut(s)	434, 461, 790, 1151
Hpy99I	CGWCG	1 cut(s)	125
HpyCH4III	ACNGT	5 cut(s)	142, 199, 292, 344, 1140
HpyCH4IV	ACGT	1 cut(s)	83
HpyCH4V	TGCA	9 cut(s)	31, 151, 655, 755, 856, 890, 902, 989, 1057
HpyF10VI	GCNNNNNNNGC	3 cut(s)	599, 954, 1217
HpyF3I	CTNAG	2 cut(s)	9, 453
HpySE526I	ACGT	1 cut(s)	83
Hsp92I	GRCGYC	1 cut(s)	1211
Hsp92II	CATG	4 cut(s)	155, 692, 731, 948
HspAI	GCGC	3 cut(s)	593, 679, 817
Ksp22I	TGATCA	2 cut(s)	717, 1123
KspAI	GTTAAC	1 cut(s)	790
Kzo9I	GATC	6 cut(s)	60, 384, 502, 717, 1123, 1186
LguI	GCTCTTC	1 cut(s)	68
LmnI	GCTCC	1 cut(s)	180
Lsp1109I	GCAGC	4 cut(s)	412, 868, 874, 1069
LweI	GCATC	3 cut(s)	661, 966, 1258
MaeI	CTAG	6 cut(s)	243, 429, 762, 785, 920, 1253
MaeII	ACGT	1 cut(s)	83
MaeIII	GTNAC	1 cut(s)	4
MalI	GATC	6 cut(s)	62, 386, 504, 719, 1125, 1188
MboI	GATC	6 cut(s)	60, 384, 502, 717, 1123, 1186
MboII	GAAGA	7 cut(s)	85, 143, 298, 557, 566, 1066, 1124
MfeI	CAATTG	2 cut(s)	40, 1078
MflI	RGATCY	3 cut(s)	384, 502, 1186
MlsI	TGGCCA	1 cut(s)	272
MluNI	TGGCCA	1 cut(s)	272
MlyI	GAGTC	4 cut(s)	8, 748, 980, 1258
MmeI	TCCRAC	2 cut(s)	74, 1219
Mox20I	TGGCCA	1 cut(s)	272
MscI	TGGCCA	1 cut(s)	272
MseI	TTAA	7 cut(s)	216, 405, 438, 539, 789, 1089, 1194
Msp20I	TGGCCA	1 cut(s)	272
MspCI	CTTAAG	1 cut(s)	437
MspR9I	CCNGG	2 cut(s)	69, 338
MunI	CAATTG	2 cut(s)	40, 1078
Mva1269I	GAATGC	3 cut(s)	338, 475, 902
MvaI	CCWGG	2 cut(s)	69, 338
MvnI	CGCG	2 cut(s)	330, 819
MwoI	GCNNNNNNNGC	3 cut(s)	599, 954, 1217
NcoI	CCATGG	1 cut(s)	727
NdeII	GATC	6 cut(s)	60, 384, 502, 717, 1123, 1186
NlaIII	CATG	4 cut(s)	155, 692, 731, 948
NlaIV	GGNNCC	1 cut(s)	926
NsbI	TGCGCA	1 cut(s)	680
NspI	RCATGY	1 cut(s)	948
PaeI	GCATGC	1 cut(s)	948
PagI	TCATGA	1 cut(s)	688
PasI	CCCWGGG	1 cut(s)	68
PciSI	GCTCTTC	1 cut(s)	68
PctI	GAATGC	3 cut(s)	338, 475, 902
PfeI	GAWTC	5 cut(s)	262, 360, 557, 701, 1115
PkrI	GCNGC	4 cut(s)	427, 858, 889, 1059
PleI	GAGTC	4 cut(s)	8, 747, 979, 1257
PpsI	GAGTC	4 cut(s)	8, 747, 979, 1257
PshBI	ATTAAT	1 cut(s)	539
PsiI	TTATAA	1 cut(s)	863
Psp6I	CCWGG	2 cut(s)	67, 336
PspFI	CCCAGC	1 cut(s)	842
PspGI	CCWGG	2 cut(s)	67, 336
PspN4I	GGNNCC	1 cut(s)	926
PspPI	GGNCC	1 cut(s)	925
PstI	CTGCAG	2 cut(s)	858, 892
PstNI	CAGNNNCTG	2 cut(s)	1204, 1220
PsuI	RGATCY	3 cut(s)	384, 502, 1186
RsaI	GTAC	3 cut(s)	294, 1017, 1142
RsaNI	GTAC	3 cut(s)	293, 1016, 1141
SapI	GCTCTTC	1 cut(s)	68
SaqAI	TTAA	7 cut(s)	216, 405, 438, 539, 789, 1089, 1194
SatI	GCNGC	4 cut(s)	426, 857, 888, 1058
Sau3AI	GATC	6 cut(s)	60, 384, 502, 717, 1123, 1186
Sau96I	GGNCC	1 cut(s)	925
SchI	GAGTC	4 cut(s)	8, 748, 980, 1258
ScrFI	CCNGG	2 cut(s)	69, 338
SfaNI	GCATC	3 cut(s)	661, 966, 1258
SfcI	CTRYAG	2 cut(s)	854, 888
SmlI	CTYRAG	1 cut(s)	437
SmoI	CTYRAG	1 cut(s)	437
SpeI	ACTAGT	1 cut(s)	761
SphI	GCATGC	1 cut(s)	948
SsiI	CCGC	3 cut(s)	34, 328, 750
SspMI	CTAG	6 cut(s)	243, 429, 762, 785, 920, 1253
StyD4I	CCNGG	2 cut(s)	67, 336
StyI	CCWWGG	1 cut(s)	727
TaaI	ACNGT	5 cut(s)	142, 199, 292, 344, 1140
TaiI	ACGT	1 cut(s)	86
TaqII	GACCGA	1 cut(s)	624
TatI	WGTACW	2 cut(s)	1015, 1140
TfiI	GAWTC	5 cut(s)	262, 360, 557, 701, 1115
Tru1I	TTAA	7 cut(s)	216, 405, 438, 539, 789, 1089, 1194
Tru9I	TTAA	7 cut(s)	216, 405, 438, 539, 789, 1089, 1194
TscAI	CASTG	1 cut(s)	1126
TseI	GCWGC	4 cut(s)	425, 856, 887, 1057
TspDTI	ATGAA	7 cut(s)	299, 352, 482, 572, 677, 764, 1035
TspGWI	ACGGA	1 cut(s)	566
TspRI	CASTG	1 cut(s)	1126
Vha464I	CTTAAG	1 cut(s)	437
VspI	ATTAAT	1 cut(s)	539
XceI	RCATGY	1 cut(s)	948
XmiI	GTMKAC	1 cut(s)	460
XspI	CTAG	6 cut(s)	243, 429, 762, 785, 920, 1253

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.