Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0007288

Physical Location & Seq

Genomic Coordinates Reverse (-)

45088283 .. 45094030

Gene Structure 5748 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00031790.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2100 bp

ATGCAAACCGACGAAGAAGAACAACGCGTCACTCCATTCCAGCTCCAGTTCGAGAAGCCAGTCGCTTCGCAGATTAAGATCGCGGAGTGGAATCCCGAGAAGGATTTACTCGCCATGGTAACTGAAGACTCCAAGATATTACTGCACCGCTTCAATTGGCAGAGGCTCTGGACGATCTCCCCAGGGAAGAGCATAACGTCTTTGTGTTGGAGACCTGATGGTAATGCAATTGCCGTCGGGCTCGAAGATGGAACAGTCTCATTGCATGATGTTGAAGAGGATGGACAGATGACTAGAGATGAGTATGGCAGCCACTCGGTATATGAAGACCGTACTTCTCGTTTGTTTCCACCACCTCCAAGAGTTCCGCGAATGCCTGGACTGGAACGTTTTAATATCCTCTGCAGTGGAGATAAAGATGGATTCATTTGCTTTAGTATATTTGGGATCTTTCCGATAGGGAAAATTAACATACACAACTTTGGTGCTGCTAGTCCACTTAAGGATACTGAACCTGAGTGTAGACTTCTGCATGCTTCTGTTTACAAGGTTGCTTTGTCAAAACATCTTTTTTATTTGATAGTCGTGTCTTCAGGAGAATTAATTGAAGAGAGGGAAGAATCAGAAAACAGGCAGATGACTGAACCTGGTATGCATGGCTTACATTGCATAGTGCTTGATACATTTATATTTTGGAAGAGAAAAAGTGAGCTTCATCAAGTGGCGCAACAAGCTTCTAACATTGAGGAATTGGCAGAGACCATGGACTTCCAGTCCTCTCCCCAAGAGGAGTTTTTGAGTCTTTTAGGCAGTGCAAGGACTAGTCCAGCAGTTCATCAGTTTCTAGTTAACTCTCTCGGTGAAGTGGGTGTGAAGCGTGTGTCAAAGGCTGTTTGTGGTGCTGGGAAAGAACTGCAGCTTATAATCCTAAATCATCTGCAGCCTGCTGCAGAAATTATTGCATTCAGGATGGGAGAACTAAGGGGCCTTTCACGATGGAGAGCACGCTATCAGGGCATCGGTTTGGATGAGTCGCTTATCAATAATGCAACACAAAATGCTGGAATGATACTTGTACAAGTTGAATGGTTTATCAGGGTGCTTTCATCTGTGGTGCAGCAGTTTTTGAACTTCTTCAATTGGCTCTTGAAGTGTATAAAGTTACTCAATTCAGAACCCAGTGATCATCATCTTCTACTGTACAATAGGTCTGTGCCTGGTATTCATGTGTTTAATCTGGTTATCAGCATCTATTCACTTACTTACACATATGAGTTTGGCAGTGAACTTCTTGTTATATTCTTGAAGTTTCTATATGACCAAGATCCTGTTAAGCAGTTGCTGGAGGCGTCGGAAGCTGATGAGAGTATAGAAGTTGGATTGGAAACAGTGCAGAGAGTTAGAGAATTAGTTCAGTTTGGGGGATTTTCAGATTGTGAGCACCTGCAGAGGATGTTAGCCAAGGAATTTCAACAGATGGAGTCTAGTTTTAAGGAGGCTTTCCAGATGCCCTTTACTACAATTTCAAGAAAGATAGTCTGCGAAGATTCATTGCCGCTTTGCCCACTTCCACCTTTGTCAGCATCTTTGTCTACCAGTATTCCTATGTCAGTATCATGTTATGAGGATGCTTCCCATTCCGTCCCTCATCAGACTCGTCAACATATGTTTCTTGACTACATATCTTTCCATATACCAGATAAGTCCTTCTCCGGTATGGAAAATTGTATAGGCATAGTGAGGGGGATTATGCATGACTCAAGCATTGTAAAGAGGGGTTATACTTCAGTAGAAGCTGTGCTGTTGTGCATCCCTGTTGGTTATTACTGTGTGGATCTTTCTTTATATAAGGAAAGTCAGATCGTTTTATTAATAAATGAAACAACTACCACTTCTGAAGGCTCTGGTGATGCCTGTATGATGATTGTGCAAGCAAATGACCTTCCATTTGTATGTATCACAAGATCCACTAGTCTAAACTGTTGGAACTTGTATCAACTGAAGGACTCTGTTGTTCACTTGCAAATGGAGAACGAGAAGGTTCTCAGTGTACCCCATTCTGTTATTGCTCCTCTAGCAGTTAGCTGTAAGTCGTTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0007275 GO:0008150 GO:0009790 GO:0009791 GO:0009793 GO:0009908 GO:0010154 GO:0022414 GO:0032501 GO:0032502 GO:0035670 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0048316 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048467 GO:0048481 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0061458 GO:0090567 GO:0099402

KEGG Pathways

Metabolic & Signaling

ko04110 ko04111 ko04113 ko04114 ko04120 ko04914 ko05166 map04110 map04111 map04113 map04114 map04120 map04914 map05166

Pfam Domains

Protein Families

ANAPC4 ANAPC4_WD40

Protein Analysis

699

Amino Acids

78.95

Weight (kDa)

5.33

Isoelectric Point (pI)

46.94

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_THOC3	PF25174	24 - 100	4.5e-07	THOC3 beta-propeller domain
ANAPC4_WD40	PF12894	29 - 94	5.2e-23	Anaphase-promoting complex subunit 4 WD40 domain
ANAPC4	PF12896	247 - 392	1.2e-39	Anaphase-promoting complex, cyclosome, subunit 4

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007288)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G21530
fragaria_vesca	FvH4_3g04050 FvH4_3g04050 FvH4_3g04050
malus_domestica	MD10G1303000.v1.1
prunus_persica	Prupe.4G036500_v2.0.a1 Prupe.4G036500_v2.0.a1 Prupe.4G036500_v2.0.a1 Prupe.4G036500_v2.0.a1 Prupe.4G036500_v2.0.a1 Prupe.4G036500_v2.0.a1
pyrus_communis	pycom10g25560
rosa_chinensis	RchiOBHm_Chr5g0006531
rosa_laevigata	RLG00000031396 RLG00000034652
rosa_multiflora	Rmu_sc0008991.1_g000016
rosa_roxburghii	Rroxscaffold_1G00031790 Rroxscaffold_1G00069530
rosa_rugosa	Rorug04G0421700
rosa_samantha	Rh5AG049400 Rh5BG048700 Rh5CG057000 Rh5DG048000
rosa_wichuraiana	Rw5G004950 Rw5G030980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	923
AarI	CACCTGC	1 cut(s)	1452
Acc36I	ACCTGC	1 cut(s)	1452
AccI	GTMKAC	2 cut(s)	523, 1592
AccII	CGCG	3 cut(s)	27, 83, 370
AciI	CCGC	4 cut(s)	83, 148, 368, 1556
AclI	AACGTT	1 cut(s)	388
AclWI	GGATC	4 cut(s)	455, 1319, 1842, 1959
AcsI	RAATTY	1 cut(s)	1466
AcuI	CTGAAG	5 cut(s)	144, 576, 1770, 1917, 2021
AcyI	GRCGYC	1 cut(s)	1349
AfaI	GTAC	4 cut(s)	334, 1077, 1202, 2052
AflII	CTTAAG	1 cut(s)	500
AflIII	ACRYGT	1 cut(s)	25
AhdI	GACNNNNNGTC	1 cut(s)	772
AhlI	ACTAGT	2 cut(s)	821, 1970
AjnI	CCWGG	4 cut(s)	181, 376, 646, 1216
AluBI	AGCT	7 cut(s)	43, 712, 734, 919, 1358, 1796, 2085
AluI	AGCT	7 cut(s)	43, 712, 734, 919, 1358, 1796, 2085
Alw21I	GWGCWC	2 cut(s)	1006, 1443
Alw26I	GTCTC	3 cut(s)	205, 262, 752
AlwI	GGATC	4 cut(s)	455, 1319, 1842, 1959
AlwNI	CAGNNNCTG	1 cut(s)	1342
Ama87I	CYCGRG	1 cut(s)	95
AoxI	GGCC	1 cut(s)	985
ApeKI	GCWGC	6 cut(s)	309, 488, 916, 940, 947, 1117
ApoI	RAATTY	1 cut(s)	1466
ArsI	GACNNNNNNTTYG	2 cut(s)	1931, 1963
AseI	ATTAAT	2 cut(s)	602, 1871
Asp700I	GAANNNNTTC	2 cut(s)	1133, 1410
AspLEI	GCGC	1 cut(s)	727
AspS9I	GGNCC	1 cut(s)	985
AsuHPI	GGTGA	2 cut(s)	872, 1919
AvaI	CYCGRG	1 cut(s)	95
BanII	GRGCYC	1 cut(s)	243
BbsI	GAAGAC	3 cut(s)	132, 333, 582
Bbv12I	GWGCWC	2 cut(s)	1006, 1443
BbvI	GCAGC	6 cut(s)	321, 475, 928, 934, 952, 1129
BccI	CCATC	7 cut(s)	212, 242, 275, 413, 964, 990, 1471
BceAI	ACGGC	1 cut(s)	218
BciT130I	CCWGG	4 cut(s)	183, 378, 648, 1218
BciVI	GTATCC	1 cut(s)	499
BclI	TGATCA	1 cut(s)	1183
BcoDI	GTCTC	3 cut(s)	205, 262, 752
BcuI	ACTAGT	2 cut(s)	821, 1970
BfaI	CTAG	7 cut(s)	294, 492, 822, 845, 1485, 1971, 2075
BfmI	CTRYAG	5 cut(s)	403, 914, 938, 948, 1445
BfrI	CTTAAG	1 cut(s)	500
BfuAI	ACCTGC	1 cut(s)	1452
BfuI	GTATCC	1 cut(s)	499
BisI	GCNGC	7 cut(s)	310, 489, 917, 941, 948, 1118, 1556
BlsI	GCNGC	7 cut(s)	311, 490, 918, 942, 949, 1119, 1557
Bme1390I	CCNGG	4 cut(s)	183, 378, 648, 1218
BmeRI	GACNNNNNGTC	1 cut(s)	772
BmeT110I	CYCGRG	1 cut(s)	95
BmgT120I	GGNCC	1 cut(s)	985
BmiI	GGNNCC	1 cut(s)	986
BmrFI	CCNGG	4 cut(s)	183, 378, 648, 1218
BmrI	ACTGGG	1 cut(s)	1173
BmsI	GCATC	7 cut(s)	1026, 1257, 1497, 1592, 1618, 1818, 1900
BmuI	ACTGGG	1 cut(s)	1173
BpiI	GAAGAC	3 cut(s)	132, 333, 582
BpmI	CTGGAG	2 cut(s)	29, 1364
BpuEI	CTTGAG	1 cut(s)	1744
BsaBI	GATNNNNATC	2 cut(s)	77, 1188
BsaHI	GRCGYC	1 cut(s)	1349
BsaI	GGTCTC	2 cut(s)	205, 752
BsaJI	CCNNGG	5 cut(s)	114, 181, 182, 762, 1461
BsaWI	WCCGGW	1 cut(s)	1712
Bse1I	ACTGG	6 cut(s)	46, 59, 387, 772, 1179, 1596
Bse3DI	GCAATG	3 cut(s)	260, 664, 1550
Bse8I	GATNNNNATC	2 cut(s)	77, 1188
BseBI	CCWGG	4 cut(s)	183, 378, 648, 1218
BseDI	CCNNGG	5 cut(s)	114, 181, 182, 762, 1461
BseGI	GGATG	6 cut(s)	286, 975, 1033, 1458, 1633, 1809
BseJI	GATNNNNATC	2 cut(s)	77, 1188
BseMI	GCAATG	3 cut(s)	260, 664, 1550
BseMII	CTCAG	2 cut(s)	507, 2059
BseNI	ACTGG	6 cut(s)	46, 59, 387, 772, 1179, 1596
BseRI	GAGGAG	2 cut(s)	803, 2061
BseXI	GCAGC	6 cut(s)	321, 475, 928, 934, 952, 1129
BseYI	CCCAGC	1 cut(s)	902
BsgI	GTGCAG	3 cut(s)	128, 1136, 1412
Bsh1236I	CGCG	3 cut(s)	27, 83, 370
BshFI	GGCC	1 cut(s)	987
BsiHKAI	GWGCWC	2 cut(s)	1006, 1443
BsiHKCI	CYCGRG	1 cut(s)	95
BsiSI	CCGG	1 cut(s)	1713
BslFI	GGGAC	1 cut(s)	1628
BsmAI	GTCTC	3 cut(s)	205, 262, 752
BsmFI	GGGAC	1 cut(s)	1628
BsmI	GAATGC	2 cut(s)	378, 962
BsnI	GGCC	1 cut(s)	987
Bso31I	GGTCTC	2 cut(s)	205, 752
BsoBI	CYCGRG	1 cut(s)	95
Bsp1286I	GDGCHC	3 cut(s)	243, 1006, 1443
Bsp1407I	TGTACA	2 cut(s)	1075, 1200
Bsp143I	GATC	8 cut(s)	78, 174, 447, 1183, 1324, 1834, 1860, 1964
Bsp19I	CCATGG	2 cut(s)	114, 762
BspACI	CCGC	4 cut(s)	83, 148, 368, 1556
BspANI	GGCC	1 cut(s)	987
BspCNI	CTCAG	2 cut(s)	508, 2058
BspFNI	CGCG	3 cut(s)	27, 83, 370
BspLI	GGNNCC	1 cut(s)	986
BspMAI	CTGCAG	5 cut(s)	407, 918, 942, 952, 1449
BspMI	ACCTGC	1 cut(s)	1452
BspPI	GGATC	4 cut(s)	455, 1319, 1842, 1959
BspQI	GCTCTTC	1 cut(s)	182
BspTI	CTTAAG	1 cut(s)	500
BspTNI	GGTCTC	2 cut(s)	205, 752
BsrDI	GCAATG	3 cut(s)	260, 664, 1550
BsrGI	TGTACA	2 cut(s)	1075, 1200
BsrI	ACTGG	6 cut(s)	46, 59, 387, 772, 1179, 1596
BssECI	CCNNGG	5 cut(s)	114, 181, 182, 762, 1461
BssMI	GATC	8 cut(s)	78, 174, 447, 1183, 1324, 1834, 1860, 1964
BssNI	GRCGYC	1 cut(s)	1349
BssT1I	CCWWGG	3 cut(s)	114, 762, 1461
Bst2UI	CCWGG	4 cut(s)	183, 378, 648, 1218
Bst4CI	ACNGT	6 cut(s)	256, 332, 1200, 1390, 1829, 1982
Bst6I	CTCTTC	4 cut(s)	182, 270, 603, 692
BstACI	GRCGYC	1 cut(s)	1349
BstAFI	CTTAAG	1 cut(s)	500
BstAUI	TGTACA	2 cut(s)	1075, 1200
BstC8I	GCNNGC	4 cut(s)	534, 945, 1006, 1932
BstDEI	CTNAG	3 cut(s)	516, 980, 2045
BstDSI	CCRYGG	2 cut(s)	114, 762
BstF5I	GGATG	6 cut(s)	286, 975, 1033, 1458, 1633, 1809
BstFNI	CGCG	3 cut(s)	27, 83, 370
BstHHI	GCGC	1 cut(s)	727
BstKTI	GATC	8 cut(s)	81, 177, 450, 1186, 1327, 1837, 1863, 1967
BstMAI	GTCTC	3 cut(s)	205, 262, 752
BstMBI	GATC	8 cut(s)	78, 174, 447, 1183, 1324, 1834, 1860, 1964
BstMWI	GCNNNNNNNGC	5 cut(s)	666, 731, 1014, 1355, 2075
BstNI	CCWGG	4 cut(s)	183, 378, 648, 1218
BstNSI	RCATGY	1 cut(s)	536
BstSCI	CCNGG	4 cut(s)	181, 376, 646, 1216
BstSFI	CTRYAG	5 cut(s)	403, 914, 938, 948, 1445
BstUI	CGCG	3 cut(s)	27, 83, 370
BstV1I	GCAGC	6 cut(s)	321, 475, 928, 934, 952, 1129
BstV2I	GAAGAC	3 cut(s)	132, 333, 582
BstX2I	RGATCY	4 cut(s)	447, 1324, 1834, 1964
BstYI	RGATCY	4 cut(s)	447, 1324, 1834, 1964
BsuI	GTATCC	1 cut(s)	499
BsuRI	GGCC	1 cut(s)	987
BtgI	CCRYGG	2 cut(s)	114, 762
BtsCI	GGATG	6 cut(s)	286, 975, 1033, 1458, 1633, 1809
BtsI	GCAGTG	3 cut(s)	412, 817, 1288
BtsIMutI	CAGTG	6 cut(s)	412, 817, 1186, 1288, 1395, 2053
BveI	ACCTGC	1 cut(s)	1452
Cac8I	GCNNGC	4 cut(s)	534, 945, 1006, 1932
CaiI	CAGNNNCTG	1 cut(s)	1342
CfoI	GCGC	1 cut(s)	727
Cfr13I	GGNCC	1 cut(s)	985
CseI	GACGC	2 cut(s)	16, 1338
CsiI	ACCWGGT	1 cut(s)	646
Csp6I	GTAC	4 cut(s)	333, 1076, 1201, 2051
CviAII	CATG	8 cut(s)	115, 266, 533, 656, 763, 1226, 1617, 1754
CviQI	GTAC	4 cut(s)	333, 1076, 1201, 2051
DdeI	CTNAG	3 cut(s)	516, 980, 2045
DpnI	GATC	8 cut(s)	80, 176, 449, 1185, 1326, 1836, 1862, 1966
DpnII	GATC	8 cut(s)	78, 174, 447, 1183, 1324, 1834, 1860, 1964
DriI	GACNNNNNGTC	1 cut(s)	772
Eam1104I	CTCTTC	4 cut(s)	182, 270, 603, 692
Eam1105I	GACNNNNNGTC	1 cut(s)	772
EarI	CTCTTC	4 cut(s)	182, 270, 603, 692
Eco130I	CCWWGG	3 cut(s)	114, 762, 1461
Eco24I	GRGCYC	1 cut(s)	243
Eco31I	GGTCTC	2 cut(s)	205, 752
Eco57I	CTGAAG	5 cut(s)	144, 576, 1770, 1917, 2021
Eco88I	CYCGRG	1 cut(s)	95
EcoO109I	RGGNCCY	1 cut(s)	985
EcoRII	CCWGG	4 cut(s)	181, 376, 646, 1216
EcoT14I	CCWWGG	3 cut(s)	114, 762, 1461
EcoT22I	ATGCAT	2 cut(s)	657, 1755
EcoT38I	GRGCYC	1 cut(s)	243
ErhI	CCWWGG	3 cut(s)	114, 762, 1461
FaeI	CATG	8 cut(s)	118, 269, 536, 659, 766, 1229, 1620, 1757
FaqI	GGGAC	1 cut(s)	1628
FatI	CATG	8 cut(s)	114, 265, 532, 655, 762, 1225, 1616, 1753
FauNDI	CATATG	2 cut(s)	1270, 1665
FbaI	TGATCA	1 cut(s)	1183
FblI	GTMKAC	2 cut(s)	523, 1592
Fnu4HI	GCNGC	7 cut(s)	310, 489, 917, 941, 948, 1118, 1556
FokI	GGATG	6 cut(s)	293, 982, 1040, 1465, 1640, 1796
FriOI	GRGCYC	1 cut(s)	243
Fsp4HI	GCNGC	7 cut(s)	310, 489, 917, 941, 948, 1118, 1556
FspBI	CTAG	7 cut(s)	294, 492, 822, 845, 1485, 1971, 2075
GlaI	GCGC	1 cut(s)	726
GluI	GCNGC	7 cut(s)	310, 489, 917, 941, 948, 1118, 1556
GsaI	CCCAGC	1 cut(s)	906
GsuI	CTGGAG	2 cut(s)	29, 1364
HaeIII	GGCC	1 cut(s)	987
HapII	CCGG	1 cut(s)	1713
HgaI	GACGC	2 cut(s)	16, 1338
HhaI	GCGC	1 cut(s)	727
Hin1I	GRCGYC	1 cut(s)	1349
Hin1II	CATG	8 cut(s)	118, 269, 536, 659, 766, 1229, 1620, 1757
Hin6I	GCGC	1 cut(s)	725
HinP1I	GCGC	1 cut(s)	725
HincII	GTYRAC	2 cut(s)	850, 1661
HindII	GTYRAC	2 cut(s)	850, 1661
HindIII	AAGCTT	1 cut(s)	732
HpaI	GTTAAC	1 cut(s)	850
HpaII	CCGG	1 cut(s)	1713
HphI	GGTGA	2 cut(s)	872, 1919
Hpy166II	GTNNAC	9 cut(s)	497, 524, 544, 850, 1286, 1593, 1661, 2017, 2051
Hpy188I	TCNGA	8 cut(s)	456, 625, 1174, 1354, 1432, 1653, 1860, 1897
Hpy8I	GTNNAC	9 cut(s)	497, 524, 544, 850, 1286, 1593, 1661, 2017, 2051
Hpy99I	CGWCG	3 cut(s)	14, 239, 1354
HpyAV	CCTTC	6 cut(s)	94, 1717, 1892, 1952, 1996, 2032
HpyCH4III	ACNGT	6 cut(s)	256, 332, 1200, 1390, 1829, 1982
HpyCH4IV	ACGT	2 cut(s)	197, 388
HpyF10VI	GCNNNNNNNGC	5 cut(s)	666, 731, 1014, 1355, 2075
HpyF3I	CTNAG	3 cut(s)	516, 980, 2045
HpySE526I	ACGT	2 cut(s)	197, 388
Hsp92I	GRCGYC	1 cut(s)	1349
Hsp92II	CATG	8 cut(s)	118, 269, 536, 659, 766, 1229, 1620, 1757
HspAI	GCGC	1 cut(s)	725
Ksp22I	TGATCA	1 cut(s)	1183
KspAI	GTTAAC	1 cut(s)	850
Kzo9I	GATC	8 cut(s)	78, 174, 447, 1183, 1324, 1834, 1860, 1964
LguI	GCTCTTC	1 cut(s)	182
LmnI	GCTCC	2 cut(s)	48, 2074
Lsp1109I	GCAGC	6 cut(s)	321, 475, 928, 934, 952, 1129
LweI	GCATC	7 cut(s)	1026, 1257, 1497, 1592, 1618, 1818, 1900
MabI	ACCWGGT	1 cut(s)	646
MaeI	CTAG	7 cut(s)	294, 492, 822, 845, 1485, 1971, 2075
MaeII	ACGT	2 cut(s)	197, 388
MaeIII	GTNAC	3 cut(s)	28, 118, 1161
MalI	GATC	8 cut(s)	80, 176, 449, 1185, 1326, 1836, 1862, 1966
MboI	GATC	8 cut(s)	78, 174, 447, 1183, 1324, 1834, 1860, 1964
MfeI	CAATTG	3 cut(s)	154, 228, 1138
MflI	RGATCY	4 cut(s)	447, 1324, 1834, 1964
MhlI	GDGCHC	3 cut(s)	243, 1006, 1443
MluI	ACGCGT	1 cut(s)	25
MlyI	GAGTC	7 cut(s)	122, 808, 1040, 1490, 1648, 1751, 2000
MmeI	TCCRAC	4 cut(s)	188, 1332, 1357, 1964
Mph1103I	ATGCAT	2 cut(s)	657, 1755
MroXI	GAANNNNTTC	2 cut(s)	1133, 1410
MslI	CAYNNNNRTG	1 cut(s)	1951
MspCI	CTTAAG	1 cut(s)	500
MspI	CCGG	1 cut(s)	1713
MspR9I	CCNGG	4 cut(s)	183, 378, 648, 1218
MunI	CAATTG	3 cut(s)	154, 228, 1138
Mva1269I	GAATGC	2 cut(s)	378, 962
MvaI	CCWGG	4 cut(s)	183, 378, 648, 1218
MvnI	CGCG	3 cut(s)	27, 83, 370
MwoI	GCNNNNNNNGC	5 cut(s)	666, 731, 1014, 1355, 2075
NcoI	CCATGG	2 cut(s)	114, 762
NdeI	CATATG	2 cut(s)	1270, 1665
NdeII	GATC	8 cut(s)	78, 174, 447, 1183, 1324, 1834, 1860, 1964
NlaIII	CATG	8 cut(s)	118, 269, 536, 659, 766, 1229, 1620, 1757
NlaIV	GGNNCC	1 cut(s)	986
NmuCI	GTSAC	1 cut(s)	28
NsiI	ATGCAT	2 cut(s)	657, 1755
NspI	RCATGY	1 cut(s)	536
PaeI	GCATGC	1 cut(s)	536
PaqCI	CACCTGC	1 cut(s)	1452
PasI	CCCWGGG	1 cut(s)	182
PciSI	GCTCTTC	1 cut(s)	182
PctI	GAATGC	2 cut(s)	378, 962
PdmI	GAANNNNTTC	2 cut(s)	1133, 1410
PfeI	GAWTC	4 cut(s)	91, 423, 620, 1547
PkrI	GCNGC	7 cut(s)	311, 490, 918, 942, 949, 1119, 1557
PleI	GAGTC	7 cut(s)	122, 807, 1039, 1489, 1648, 1751, 2000
PpsI	GAGTC	7 cut(s)	122, 807, 1039, 1489, 1648, 1751, 2000
PshBI	ATTAAT	2 cut(s)	602, 1871
PsiI	TTATAA	1 cut(s)	923
Psp1406I	AACGTT	1 cut(s)	388
Psp6I	CCWGG	4 cut(s)	181, 376, 646, 1216
PspFI	CCCAGC	1 cut(s)	902
PspGI	CCWGG	4 cut(s)	181, 376, 646, 1216
PspN4I	GGNNCC	1 cut(s)	986
PspPI	GGNCC	1 cut(s)	985
PstI	CTGCAG	5 cut(s)	407, 918, 942, 952, 1449
PstNI	CAGNNNCTG	1 cut(s)	1342
PsuI	RGATCY	4 cut(s)	447, 1324, 1834, 1964
RsaI	GTAC	4 cut(s)	334, 1077, 1202, 2052
RsaNI	GTAC	4 cut(s)	333, 1076, 1201, 2051
RseI	CAYNNNNRTG	1 cut(s)	1951
SapI	GCTCTTC	1 cut(s)	182
SatI	GCNGC	7 cut(s)	310, 489, 917, 941, 948, 1118, 1556
Sau3AI	GATC	8 cut(s)	78, 174, 447, 1183, 1324, 1834, 1860, 1964
Sau96I	GGNCC	1 cut(s)	985
SchI	GAGTC	7 cut(s)	122, 808, 1040, 1490, 1648, 1751, 2000
ScrFI	CCNGG	4 cut(s)	183, 378, 648, 1218
SduI	GDGCHC	3 cut(s)	243, 1006, 1443
SexAI	ACCWGGT	1 cut(s)	646
SfaNI	GCATC	7 cut(s)	1026, 1257, 1497, 1592, 1618, 1818, 1900
SfcI	CTRYAG	5 cut(s)	403, 914, 938, 948, 1445
SmiMI	CAYNNNNRTG	1 cut(s)	1951
SmlI	CTYRAG	2 cut(s)	500, 1759
SmoI	CTYRAG	2 cut(s)	500, 1759
SpeI	ACTAGT	2 cut(s)	821, 1970
SphI	GCATGC	1 cut(s)	536
SsiI	CCGC	4 cut(s)	83, 148, 368, 1556
SspMI	CTAG	7 cut(s)	294, 492, 822, 845, 1485, 1971, 2075
StyD4I	CCNGG	4 cut(s)	181, 376, 646, 1216
StyI	CCWWGG	3 cut(s)	114, 762, 1461
TaaI	ACNGT	6 cut(s)	256, 332, 1200, 1390, 1829, 1982
TaiI	ACGT	2 cut(s)	200, 391
TaqI	TCGA	2 cut(s)	51, 243
TatI	WGTACW	2 cut(s)	1075, 1200
TauI	GCSGC	1 cut(s)	1558
TfiI	GAWTC	4 cut(s)	91, 423, 620, 1547
TscAI	CASTG	6 cut(s)	412, 817, 1186, 1288, 1395, 2053
TseFI	GTSAC	1 cut(s)	28
TseI	GCWGC	6 cut(s)	309, 488, 916, 940, 947, 1117
Tsp45I	GTSAC	1 cut(s)	28
TspDTI	ATGAA	8 cut(s)	339, 415, 704, 824, 1095, 1214, 1539, 1893
TspGWI	ACGGA	1 cut(s)	1630
TspRI	CASTG	6 cut(s)	412, 817, 1186, 1288, 1395, 2053
Vha464I	CTTAAG	1 cut(s)	500
VspI	ATTAAT	2 cut(s)	602, 1871
XapI	RAATTY	1 cut(s)	1466
XceI	RCATGY	1 cut(s)	536
XmiI	GTMKAC	2 cut(s)	523, 1592
XmnI	GAANNNNTTC	2 cut(s)	1133, 1410
XspI	CTAG	7 cut(s)	294, 492, 822, 845, 1485, 1971, 2075
Zsp2I	ATGCAT	2 cut(s)	657, 1755

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.