Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0007288

Physical Location & Seq

Genomic Coordinates Reverse (-)

38962813 .. 38976156

Gene Structure 13344 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1303000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2331 bp

ATGGAAACGGACGAAGCCCAGCGAGTTCTTCCCTTTCAGCTCCAGTTCGACAAGCCACTCGCGTCTCAGATTAAAATTGCAGAATGGAACCCTGAGAAGGATTTGCTGGCGATGGTCACCGAAGACTCCAAGATTTTGCTGCACCGTTTCAATTGGCAGAGGCTCTGGACAATCTCTCCCGCAAGGAGCATATCGTCGTTGTGTTGGCGTCCCGATGGAAAAGCAATTGCTGTTGGGCTTGAAGATGGAACAGTTTCATTGCATGATGTTGAAAATGGAAAGTTGTTGAGAAGCTTAAAATCACATTCCGTTGCTGTTGTTTCACTTAACTGGGAGGAGGATGGACACACGACTAGAGATGAGCACGGCAGTATATCAACATATGAAGACCGTACTTCTCGTTTCTTCCCACCTCCTCCAAGAGTTCCGCGGATGCCTGGACTGGTATCTGGAGATACTGGTTTCATAGATGATAGTGAAGATTCATTTCAAGAGCTGTCAAATTCTTCACAGCAACGTTTTAACATCCTGTGTAGTGGAGACAAAGATGGAGTCATTTGCTTTAGTATATTTGGGATTTTTCCAATAGGGAAAATTAACATACACAATTTTTGTGTTCCTAATCCACTTACGGATGCAAAAGCTGAGTGCAGACTTCTTAATGCTTCTGTTTGCAAGGTTGCTTTGTCGAAAGATCTTTGTCATTTGATAGTCACATGTTCAGGAGAATTGACTGATGATAGGGAGGAATCAGAAAGCAGACGAGTGACTCAACATGGTATTCATGGCTTACACTGCATGGTGCTTGATACATCTATATTTTGGAAGAGGAAAAATGAGCTTCACCAAGTGGCTCAACAAGCGTCTAACATTGAGGAATTGGTGGAGGTTATTCGGGCATCACTTTCAGTTATGCACAAGCAGTGGTCTGATGCTATGCACACTTTCCATGAAAAGTTTGATTCTCTATCTAATCTGATTGTTGACCATGGACTTGACTCTTGTCCCCAGGAGGAGTTTTTGAGTCTTTTAGGTGGTGCAAGGACTAGTCCACCTGTTCATCAATTTCTTGTTAACTCTCTTGGTGAAGTGGGCGTGAAGCGTGTGTCAAAGGCTGTTTGTGGTGCCGGAAAAGAACTCCAGCTTATTGTCCTAAATCATCTCCAGCCTGCTGCAGAAATTATTGCATTCAGGATGGGAGAATTAAGAGGCCTTTCAAGATGGCGAGCACGATATCAGGGAATTGGTTTGGATGAGACACTCATCAATAATGCAACAGAAAAAGCTGGGATGATACTTTTACAAGTTGAACGATTTATCAGGGTGCTGTCAACTGTTGTGCAGCAGTTTTCAAATTTCTTCAACTGGCTCTTGAAATGTATAAAGTTACTTATGTCAGAACCAAGCGATCACCATCTTCTACTGTACAATAGTGAACTTGTCGTTATATTCTTGAAGTTTTTATATGACCAAGATCCTGTCAAGCAGTTGCTGGAAGCATCAGAAGCCGATCACCATATCGATATTGGTTTGGAAACAATGCAAAGAGTTAAAGAACTGGTTCAGTTTGGGGGATTTTCAGATTTTGGATATCTGCAAAGGACATTAGCAAAGGAATCCCAGCAGATGGAGTCTAGTTTCAAGGAGGCTTTTCTGATGCCCTTTACAACAATATCAAGAAAGATACTGTGCAAGGATTTATTGCCGCTTTGCCCTCTTCCACCTTTGTCATCATCTTTGTCAACCACCATTCCTGTTTCAGTATCGTATTATGAGGATGCCTCCCAATCGGTCTCTTCTTGTCAGAGTGAACACTTGTTTCTCGATTACATATCTTTCCAAATACCAGATGAGTCTCTCTCCGGAATTGCAAATTGTATAGGAATAGTGAAAGGGTTTATGCATGACTCGAGCAGTGTAAACAAGGGCTATACTTCATTAGAAGCTGTATTGTTGGGGGTCCCTGCTGGTTATTACTGCATGGATCTTTCCTTATATAAGGAAAGTCAGATTGTTTTGTTATTAAATGAAACAACCGCCGCTTCTGAAAGTTCTGGAGACGCCTCTATGATGATTGTGCAAACAGATGACCTTCCATTTGTATCGATCTCAAGATCCACAGGTCTAAACTATTGGACGTTGCATCAACTAAAGGACTCTGTTGTTCACCTGGAAATGGAGAATGAGAAGGTGCGCAATATACCTCACTCCATCGTTGCACCCCTCGCAGTCAGCTCATCAAGAGGCGTGGCCTGTGTTTTCGCTGCAAGAAAACGTGCCTTGGTCTACATTCTGGATGAAGACGAAGATGAAGTCTCAGATGCAGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0007275 GO:0008150 GO:0009790 GO:0009791 GO:0009793 GO:0009908 GO:0010154 GO:0022414 GO:0032501 GO:0032502 GO:0035670 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0048316 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048467 GO:0048481 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0061458 GO:0090567 GO:0099402

KEGG Pathways

Metabolic & Signaling

ko04110 ko04111 ko04113 ko04114 ko04120 ko04914 ko05166 map04110 map04111 map04113 map04114 map04120 map04914 map05166

Pfam Domains

Protein Families

ANAPC4 ANAPC4_WD40

Protein Analysis

777

Amino Acids

86.76

Weight (kDa)

5.3

Isoelectric Point (pI)

48.25

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_EML_2	PF23414	19 - 119	1.4e-08	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR19_1st	PF23389	20 - 116	1.8e-07	WDR19 first beta-propeller
WD40_WDHD1_1st	PF24817	22 - 116	4.3e-09	WDHD1 first WD40 domain
Beta-prop_WDR35_TULP_N	PF24797	22 - 113	5.6e-07	WDR35/TULP4 N-terminal
Beta-prop_THOC3	PF25174	23 - 117	3.9e-10	THOC3 beta-propeller domain
WD40_Prp19	PF24814	23 - 116	5.9e-08	Prp19 WD40 domain
Beta-prop_WDR5	PF25175	24 - 116	1.4e-08	WDR5 beta-propeller domain
WD40_CDC20-Fz	PF24807	26 - 134	5.8e-12	CDC20/Fizzy WD40 domain
WDR55	PF24796	26 - 133	5.9e-07	WDR55
ANAPC4_WD40	PF12894	28 - 113	1.4e-28	Anaphase-promoting complex subunit 4 WD40 domain
Beta-prop_WDR3_1st	PF25173	61 - 117	8.4e-08	WDR3 first beta-propeller domain
ANAPC4	PF12896	265 - 467	2.4e-57	Anaphase-promoting complex, cyclosome, subunit 4

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0007288)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G21530
fragaria_vesca	FvH4_3g04050 FvH4_3g04050 FvH4_3g04050
malus_domestica	MD10G1303000.v1.1
prunus_persica	Prupe.4G036500_v2.0.a1 Prupe.4G036500_v2.0.a1 Prupe.4G036500_v2.0.a1 Prupe.4G036500_v2.0.a1 Prupe.4G036500_v2.0.a1 Prupe.4G036500_v2.0.a1
pyrus_communis	pycom10g25560
rosa_chinensis	RchiOBHm_Chr5g0006531
rosa_laevigata	RLG00000031396 RLG00000034652
rosa_multiflora	Rmu_sc0008991.1_g000016
rosa_roxburghii	Rroxscaffold_1G00031790 Rroxscaffold_1G00069530
rosa_rugosa	Rorug04G0421700
rosa_samantha	Rh5AG049400 Rh5BG048700 Rh5CG057000 Rh5DG048000
rosa_wichuraiana	Rw5G004950 Rw5G030980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	2195
AccB1I	GGYRCC	1 cut(s)	1124
AccB7I	CCANNNNNTGG	1 cut(s)	1627
AccI	GTMKAC	1 cut(s)	2286
AccII	CGCG	2 cut(s)	62, 430
AccIII	TCCGGA	1 cut(s)	1862
AciI	CCGC	6 cut(s)	180, 428, 430, 1706, 2037, 2040
AclI	AACGTT	1 cut(s)	517
AclWI	GGATC	3 cut(s)	1469, 1992, 2109
AcsI	RAATTY	2 cut(s)	502, 1354
AcyI	GRCGYC	2 cut(s)	208, 2061
AdeI	CACNNNGTG	1 cut(s)	850
AfaI	GTAC	2 cut(s)	394, 1427
AfiI	CCNNNNNNNGG	3 cut(s)	97, 1627, 2176
AflIII	ACRYGT	1 cut(s)	716
AhlI	ACTAGT	1 cut(s)	1046
AjnI	CCWGG	3 cut(s)	436, 1008, 2169
AluBI	AGCT	9 cut(s)	40, 294, 496, 644, 841, 1144, 1286, 1946, 2235
AluI	AGCT	9 cut(s)	40, 294, 496, 644, 841, 1144, 1286, 1946, 2235
Alw21I	GWGCWC	2 cut(s)	366, 1231
Alw26I	GTCTC	7 cut(s)	69, 534, 1250, 1798, 1860, 2052, 2320
AlwI	GGATC	3 cut(s)	1469, 1992, 2109
AlwNI	CAGNNNCTG	1 cut(s)	1492
Ama87I	CYCGRG	1 cut(s)	1909
Aor13HI	TCCGGA	1 cut(s)	1862
AoxI	GGCC	2 cut(s)	1210, 2250
ApeKI	GCWGC	4 cut(s)	139, 1172, 1342, 2264
ApoI	RAATTY	2 cut(s)	502, 1354
ArsI	GACNNNNNNTTYG	6 cut(s)	537, 569, 2081, 2113, 2298, 2330
Asp700I	GAANNNNTTC	2 cut(s)	253, 1560
AspLEI	GCGC	1 cut(s)	2196
AspS9I	GGNCC	1 cut(s)	1960
AsuHPI	GGTGA	6 cut(s)	109, 836, 1097, 1403, 1505, 2159
AvaI	CYCGRG	1 cut(s)	1909
AvaII	GGWCC	1 cut(s)	1960
BanI	GGYRCC	1 cut(s)	1124
BbsI	GAAGAC	3 cut(s)	129, 393, 2307
Bbv12I	GWGCWC	2 cut(s)	366, 1231
BbvI	GCAGC	4 cut(s)	126, 1159, 1354, 2251
BceAI	ACGGC	1 cut(s)	382
BciT130I	CCWGG	3 cut(s)	438, 1010, 2171
BcoDI	GTCTC	7 cut(s)	69, 534, 1250, 1798, 1860, 2052, 2320
BcuI	ACTAGT	1 cut(s)	1046
BfaI	CTAG	3 cut(s)	354, 1047, 1635
BfmI	CTRYAG	1 cut(s)	1173
BglII	AGATCT	1 cut(s)	694
BisI	GCNGC	6 cut(s)	140, 1173, 1343, 1706, 2040, 2265
BlsI	GCNGC	6 cut(s)	141, 1174, 1344, 1707, 2041, 2266
Bme1390I	CCNGG	3 cut(s)	438, 1010, 2171
Bme18I	GGWCC	1 cut(s)	1960
BmeT110I	CYCGRG	1 cut(s)	1909
BmgT120I	GGNCC	1 cut(s)	1960
BmiI	GGNNCC	4 cut(s)	89, 1126, 1961, 1962
BmrFI	CCNGG	3 cut(s)	438, 1010, 2171
BmrI	ACTGGG	1 cut(s)	340
BmsI	GCATC	9 cut(s)	423, 625, 908, 922, 1508, 1647, 1768, 2152, 2311
BmuI	ACTGGG	1 cut(s)	340
BoxI	GACNNNNGTC	1 cut(s)	1002
BpiI	GAAGAC	3 cut(s)	129, 393, 2307
BplI	GAGNNNNNCTC	4 cut(s)	1766, 1798, 1844, 1876
BpmI	CTGGAG	5 cut(s)	26, 471, 1124, 1148, 2076
BpuEI	CTTGAG	1 cut(s)	2095
Bsa29I	ATCGAT	2 cut(s)	1521, 2105
BsaBI	GATNNNNATC	1 cut(s)	1413
BsaHI	GRCGYC	2 cut(s)	208, 2061
BsaI	GGTCTC	1 cut(s)	1798
BsaJI	CCNNGG	4 cut(s)	428, 988, 1008, 2280
BsaWI	WCCGGW	1 cut(s)	1862
BsaXI	ACNNNNNCTCC	2 cut(s)	160, 190
Bsc4I	CCNNNNNNNGG	3 cut(s)	97, 1627, 2176
Bse1I	ACTGG	6 cut(s)	43, 335, 447, 463, 1370, 1563
Bse3DI	GCAATG	1 cut(s)	257
Bse8I	GATNNNNATC	1 cut(s)	1413
BseAI	TCCGGA	1 cut(s)	1862
BseBI	CCWGG	3 cut(s)	438, 1010, 2171
BseCI	ATCGAT	2 cut(s)	1521, 2105
BseDI	CCNNGG	4 cut(s)	428, 988, 1008, 2280
BseGI	GGATG	9 cut(s)	346, 438, 525, 640, 1200, 1258, 1296, 1783, 2302
BseJI	GATNNNNATC	1 cut(s)	1413
BseLI	CCNNNNNNNGG	3 cut(s)	97, 1627, 2176
BseMI	GCAATG	1 cut(s)	257
BseMII	CTCAG	4 cut(s)	80, 84, 636, 2331
BseNI	ACTGG	6 cut(s)	43, 335, 447, 463, 1370, 1563
BseRI	GAGGAG	3 cut(s)	350, 405, 1028
BseXI	GCAGC	4 cut(s)	126, 1159, 1354, 2251
BseYI	CCCAGC	3 cut(s)	18, 1286, 1620
BsgI	GTGCAG	3 cut(s)	125, 670, 1361
Bsh1236I	CGCG	2 cut(s)	62, 430
BshFI	GGCC	2 cut(s)	1212, 2252
BshNI	GGYRCC	1 cut(s)	1124
BshVI	ATCGAT	2 cut(s)	1521, 2105
BsiHKAI	GWGCWC	2 cut(s)	366, 1231
BsiHKCI	CYCGRG	1 cut(s)	1909
BsiSI	CCGG	2 cut(s)	1128, 1863
BslFI	GGGAC	3 cut(s)	195, 990, 1946
BslI	CCNNNNNNNGG	3 cut(s)	97, 1627, 2176
BsmAI	GTCTC	7 cut(s)	69, 534, 1250, 1798, 1860, 2052, 2320
BsmBI	CGTCTC	2 cut(s)	69, 2052
BsmFI	GGGAC	3 cut(s)	195, 990, 1946
BsmI	GAATGC	1 cut(s)	1187
BsnI	GGCC	2 cut(s)	1212, 2252
Bso31I	GGTCTC	1 cut(s)	1798
BsoBI	CYCGRG	1 cut(s)	1909
Bsp1286I	GDGCHC	2 cut(s)	366, 1231
Bsp13I	TCCGGA	1 cut(s)	1862
Bsp1407I	TGTACA	1 cut(s)	1425
Bsp143I	GATC	7 cut(s)	694, 1408, 1474, 1510, 1984, 2106, 2114
Bsp19I	CCATGG	1 cut(s)	988
BspACI	CCGC	6 cut(s)	180, 428, 430, 1706, 2037, 2040
BspANI	GGCC	2 cut(s)	1212, 2252
BspCNI	CTCAG	4 cut(s)	79, 85, 637, 2330
BspDI	ATCGAT	2 cut(s)	1521, 2105
BspEI	TCCGGA	1 cut(s)	1862
BspFNI	CGCG	2 cut(s)	62, 430
BspLI	GGNNCC	4 cut(s)	89, 1126, 1961, 1962
BspMAI	CTGCAG	1 cut(s)	1177
BspPI	GGATC	3 cut(s)	1469, 1992, 2109
BspT107I	GGYRCC	1 cut(s)	1124
BspTNI	GGTCTC	1 cut(s)	1798
BsrDI	GCAATG	1 cut(s)	257
BsrGI	TGTACA	1 cut(s)	1425
BsrI	ACTGG	6 cut(s)	43, 335, 447, 463, 1370, 1563
BssECI	CCNNGG	4 cut(s)	428, 988, 1008, 2280
BssMI	GATC	7 cut(s)	694, 1408, 1474, 1510, 1984, 2106, 2114
BssNI	GRCGYC	2 cut(s)	208, 2061
BssT1I	CCWWGG	2 cut(s)	988, 2280
Bst2UI	CCWGG	3 cut(s)	438, 1010, 2171
Bst4CI	ACNGT	6 cut(s)	146, 253, 392, 1336, 1425, 1689
Bst6I	CTCTTC	3 cut(s)	821, 1722, 1801
BstACI	GRCGYC	2 cut(s)	208, 2061
BstAUI	TGTACA	1 cut(s)	1425
BstC8I	GCNNGC	3 cut(s)	108, 1170, 1227
BstDEI	CTNAG	4 cut(s)	66, 93, 645, 2317
BstDSI	CCRYGG	2 cut(s)	428, 988
BstEII	GGTNACC	1 cut(s)	115
BstF5I	GGATG	9 cut(s)	346, 438, 525, 640, 1200, 1258, 1296, 1783, 2302
BstFNI	CGCG	2 cut(s)	62, 430
BstHHI	GCGC	1 cut(s)	2196
BstKTI	GATC	7 cut(s)	697, 1411, 1477, 1513, 1987, 2109, 2117
BstMAI	GTCTC	7 cut(s)	69, 534, 1250, 1798, 1860, 2052, 2320
BstMBI	GATC	7 cut(s)	694, 1408, 1474, 1510, 1984, 2106, 2114
BstMWI	GCNNNNNNNGC	4 cut(s)	795, 860, 1505, 2225
BstNI	CCWGG	3 cut(s)	438, 1010, 2171
BstNSI	RCATGY	1 cut(s)	720
BstPAI	GACNNNNGTC	1 cut(s)	1002
BstPI	GGTNACC	1 cut(s)	115
BstSCI	CCNGG	3 cut(s)	436, 1008, 2169
BstSFI	CTRYAG	1 cut(s)	1173
BstUI	CGCG	2 cut(s)	62, 430
BstV1I	GCAGC	4 cut(s)	126, 1159, 1354, 2251
BstV2I	GAAGAC	3 cut(s)	129, 393, 2307
BstX2I	RGATCY	4 cut(s)	694, 1474, 1984, 2114
BstYI	RGATCY	4 cut(s)	694, 1474, 1984, 2114
Bsu15I	ATCGAT	2 cut(s)	1521, 2105
BsuRI	GGCC	2 cut(s)	1212, 2252
BsuTUI	ATCGAT	2 cut(s)	1521, 2105
BtgI	CCRYGG	2 cut(s)	428, 988
BtgZI	GCGATG	1 cut(s)	125
BtsCI	GGATG	9 cut(s)	346, 438, 525, 640, 1200, 1258, 1296, 1783, 2302
BtsI	GCAGTG	3 cut(s)	793, 929, 1921
BtsIMutI	CAGTG	3 cut(s)	793, 929, 1921
Cac8I	GCNNGC	3 cut(s)	108, 1170, 1227
CaiI	CAGNNNCTG	1 cut(s)	1492
CfoI	GCGC	1 cut(s)	2196
Cfr13I	GGNCC	1 cut(s)	1960
Cfr42I	CCGCGG	1 cut(s)	431
ClaI	ATCGAT	2 cut(s)	1521, 2105
CseI	GACGC	4 cut(s)	51, 197, 852, 2069
Csp6I	GTAC	2 cut(s)	393, 1426
CspCI	CAANNNNNGTGG	2 cut(s)	2229, 2264
CviAII	CATG	9 cut(s)	263, 717, 776, 785, 799, 950, 989, 1904, 1981
CviQI	GTAC	2 cut(s)	393, 1426
DdeI	CTNAG	4 cut(s)	66, 93, 645, 2317
DpnI	GATC	7 cut(s)	696, 1410, 1476, 1512, 1986, 2108, 2116
DpnII	GATC	7 cut(s)	694, 1408, 1474, 1510, 1984, 2106, 2114
DraIII	CACNNNGTG	1 cut(s)	850
Eam1104I	CTCTTC	3 cut(s)	821, 1722, 1801
EarI	CTCTTC	3 cut(s)	821, 1722, 1801
Eco130I	CCWWGG	2 cut(s)	988, 2280
Eco147I	AGGCCT	1 cut(s)	1212
Eco31I	GGTCTC	1 cut(s)	1798
Eco32I	GATATC	2 cut(s)	1235, 1592
Eco47I	GGWCC	1 cut(s)	1960
Eco88I	CYCGRG	1 cut(s)	1909
Eco91I	GGTNACC	1 cut(s)	115
EcoO109I	RGGNCCY	1 cut(s)	1960
EcoO65I	GGTNACC	1 cut(s)	115
EcoRII	CCWGG	3 cut(s)	436, 1008, 2169
EcoRV	GATATC	2 cut(s)	1235, 1592
EcoT14I	CCWWGG	2 cut(s)	988, 2280
EcoT22I	ATGCAT	1 cut(s)	1905
ErhI	CCWWGG	2 cut(s)	988, 2280
Esp3I	CGTCTC	2 cut(s)	69, 2052
FaeI	CATG	9 cut(s)	266, 720, 779, 788, 802, 953, 992, 1907, 1984
FaqI	GGGAC	3 cut(s)	195, 990, 1946
FatI	CATG	9 cut(s)	262, 716, 775, 784, 798, 949, 988, 1903, 1980
FauI	CCCGC	1 cut(s)	187
FauNDI	CATATG	1 cut(s)	382
FblI	GTMKAC	1 cut(s)	2286
Fnu4HI	GCNGC	6 cut(s)	140, 1173, 1343, 1706, 2040, 2265
FokI	GGATG	9 cut(s)	353, 445, 512, 647, 1207, 1265, 1303, 1790, 2309
Fsp4HI	GCNGC	6 cut(s)	140, 1173, 1343, 1706, 2040, 2265
FspBI	CTAG	3 cut(s)	354, 1047, 1635
FspI	TGCGCA	1 cut(s)	2195
GlaI	GCGC	1 cut(s)	2195
GluI	GCNGC	6 cut(s)	140, 1173, 1343, 1706, 2040, 2265
GsaI	CCCAGC	3 cut(s)	22, 1290, 1624
GsuI	CTGGAG	5 cut(s)	26, 471, 1124, 1148, 2076
HaeIII	GGCC	2 cut(s)	1212, 2252
HapII	CCGG	2 cut(s)	1128, 1863
HgaI	GACGC	4 cut(s)	51, 197, 852, 2069
HhaI	GCGC	1 cut(s)	2196
Hin1I	GRCGYC	2 cut(s)	208, 2061
Hin1II	CATG	9 cut(s)	266, 720, 779, 788, 802, 953, 992, 1907, 1984
Hin6I	GCGC	1 cut(s)	2194
HinP1I	GCGC	1 cut(s)	2194
HincII	GTYRAC	4 cut(s)	985, 1075, 1332, 1743
HindII	GTYRAC	4 cut(s)	985, 1075, 1332, 1743
HindIII	AAGCTT	1 cut(s)	292
HpaI	GTTAAC	1 cut(s)	1075
HpaII	CCGG	2 cut(s)	1128, 1863
HphI	GGTGA	6 cut(s)	109, 836, 1097, 1403, 1505, 2159
Hpy99I	CGWCG	1 cut(s)	199
HpyAV	CCTTC	3 cut(s)	91, 2102, 2182
HpyCH4III	ACNGT	6 cut(s)	146, 253, 392, 1336, 1425, 1689
HpyCH4IV	ACGT	3 cut(s)	517, 2138, 2275
HpyF10VI	GCNNNNNNNGC	4 cut(s)	795, 860, 1505, 2225
HpyF3I	CTNAG	4 cut(s)	66, 93, 645, 2317
HpySE526I	ACGT	3 cut(s)	517, 2138, 2275
Hsp92I	GRCGYC	2 cut(s)	208, 2061
Hsp92II	CATG	9 cut(s)	266, 720, 779, 788, 802, 953, 992, 1907, 1984
HspAI	GCGC	1 cut(s)	2194
KflI	GGGWCCC	1 cut(s)	1960
Kpn2I	TCCGGA	1 cut(s)	1862
KspAI	GTTAAC	1 cut(s)	1075
KspI	CCGCGG	1 cut(s)	431
Kzo9I	GATC	7 cut(s)	694, 1408, 1474, 1510, 1984, 2106, 2114
LmnI	GCTCC	2 cut(s)	45, 186
Lsp1109I	GCAGC	4 cut(s)	126, 1159, 1354, 2251
LweI	GCATC	9 cut(s)	423, 625, 908, 922, 1508, 1647, 1768, 2152, 2311
MaeI	CTAG	3 cut(s)	354, 1047, 1635
MaeII	ACGT	3 cut(s)	517, 2138, 2275
MaeIII	GTNAC	4 cut(s)	115, 712, 766, 1386
MalI	GATC	7 cut(s)	696, 1410, 1476, 1512, 1986, 2108, 2116
MboI	GATC	7 cut(s)	694, 1408, 1474, 1510, 1984, 2106, 2114
MfeI	CAATTG	2 cut(s)	151, 225
MflI	RGATCY	4 cut(s)	694, 1474, 1984, 2114
MhlI	GDGCHC	2 cut(s)	366, 1231
MlyI	GAGTC	9 cut(s)	119, 561, 763, 992, 1033, 1640, 1862, 1901, 2150
Mph1103I	ATGCAT	1 cut(s)	1905
MroI	TCCGGA	1 cut(s)	1862
MroXI	GAANNNNTTC	2 cut(s)	253, 1560
MspA1I	CMGCKG	1 cut(s)	430
MspI	CCGG	2 cut(s)	1128, 1863
MspR9I	CCNGG	3 cut(s)	438, 1010, 2171
MunI	CAATTG	2 cut(s)	151, 225
Mva1269I	GAATGC	1 cut(s)	1187
MvaI	CCWGG	3 cut(s)	438, 1010, 2171
MvnI	CGCG	2 cut(s)	62, 430
MwoI	GCNNNNNNNGC	4 cut(s)	795, 860, 1505, 2225
NcoI	CCATGG	1 cut(s)	988
NdeI	CATATG	1 cut(s)	382
NdeII	GATC	7 cut(s)	694, 1408, 1474, 1510, 1984, 2106, 2114
NlaIII	CATG	9 cut(s)	266, 720, 779, 788, 802, 953, 992, 1907, 1984
NlaIV	GGNNCC	4 cut(s)	89, 1126, 1961, 1962
NmuCI	GTSAC	3 cut(s)	115, 712, 766
NsbI	TGCGCA	1 cut(s)	2195
NsiI	ATGCAT	1 cut(s)	1905
NspI	RCATGY	1 cut(s)	720
PaeR7I	CTCGAG	1 cut(s)	1909
PceI	AGGCCT	1 cut(s)	1212
PciI	ACATGT	1 cut(s)	716
PctI	GAATGC	1 cut(s)	1187
PdmI	GAANNNNTTC	2 cut(s)	253, 1560
PfeI	GAWTC	4 cut(s)	482, 749, 962, 1616
PflMI	CCANNNNNTGG	1 cut(s)	1627
PkrI	GCNGC	6 cut(s)	141, 1174, 1344, 1707, 2041, 2266
PleI	GAGTC	9 cut(s)	119, 560, 763, 992, 1032, 1639, 1861, 1901, 2150
PpsI	GAGTC	9 cut(s)	119, 560, 763, 992, 1032, 1639, 1861, 1901, 2150
PpuMI	RGGWCCY	1 cut(s)	1960
PscI	ACATGT	1 cut(s)	716
PshAI	GACNNNNGTC	1 cut(s)	1002
Psp1406I	AACGTT	1 cut(s)	517
Psp5II	RGGWCCY	1 cut(s)	1960
Psp6I	CCWGG	3 cut(s)	436, 1008, 2169
PspEI	GGTNACC	1 cut(s)	115
PspFI	CCCAGC	3 cut(s)	18, 1286, 1620
PspGI	CCWGG	3 cut(s)	436, 1008, 2169
PspN4I	GGNNCC	4 cut(s)	89, 1126, 1961, 1962
PspPI	GGNCC	1 cut(s)	1960
PspPPI	RGGWCCY	1 cut(s)	1960
PspXI	VCTCGAGB	1 cut(s)	1909
PstI	CTGCAG	1 cut(s)	1177
PstNI	CAGNNNCTG	1 cut(s)	1492
PsuI	RGATCY	4 cut(s)	694, 1474, 1984, 2114
RsaI	GTAC	2 cut(s)	394, 1427
RsaNI	GTAC	2 cut(s)	393, 1426
SacII	CCGCGG	1 cut(s)	431
SatI	GCNGC	6 cut(s)	140, 1173, 1343, 1706, 2040, 2265
Sau3AI	GATC	7 cut(s)	694, 1408, 1474, 1510, 1984, 2106, 2114
Sau96I	GGNCC	1 cut(s)	1960
SchI	GAGTC	9 cut(s)	119, 561, 763, 992, 1033, 1640, 1862, 1901, 2150
ScrFI	CCNGG	3 cut(s)	438, 1010, 2171
SduI	GDGCHC	2 cut(s)	366, 1231
SfaNI	GCATC	9 cut(s)	423, 625, 908, 922, 1508, 1647, 1768, 2152, 2311
SfcI	CTRYAG	1 cut(s)	1173
Sfr274I	CTCGAG	1 cut(s)	1909
Sfr303I	CCGCGG	1 cut(s)	431
SgrBI	CCGCGG	1 cut(s)	431
SinI	GGWCC	1 cut(s)	1960
SlaI	CTCGAG	1 cut(s)	1909
SmlI	CTYRAG	2 cut(s)	1909, 2110
SmoI	CTYRAG	2 cut(s)	1909, 2110
SpeI	ACTAGT	1 cut(s)	1046
SseBI	AGGCCT	1 cut(s)	1212
SsiI	CCGC	6 cut(s)	180, 428, 430, 1706, 2037, 2040
SspMI	CTAG	3 cut(s)	354, 1047, 1635
StuI	AGGCCT	1 cut(s)	1212
StyD4I	CCNGG	3 cut(s)	436, 1008, 2169
StyI	CCWWGG	2 cut(s)	988, 2280
TaaI	ACNGT	6 cut(s)	146, 253, 392, 1336, 1425, 1689
TaiI	ACGT	3 cut(s)	520, 2141, 2278
TaqI	TCGA	6 cut(s)	48, 689, 1521, 1824, 1910, 2105
TaqII	GACCGA	1 cut(s)	1780
TatI	WGTACW	1 cut(s)	1425
TauI	GCSGC	2 cut(s)	1708, 2042
TfiI	GAWTC	4 cut(s)	482, 749, 962, 1616
TscAI	CASTG	3 cut(s)	800, 929, 1921
TseFI	GTSAC	3 cut(s)	115, 712, 766
TseI	GCWGC	4 cut(s)	139, 1172, 1342, 2264
Tsp45I	GTSAC	3 cut(s)	115, 712, 766
TspGWI	ACGGA	3 cut(s)	23, 298, 647
TspRI	CASTG	3 cut(s)	800, 929, 1921
Van91I	CCANNNNNTGG	1 cut(s)	1627
VpaK11BI	GGWCC	1 cut(s)	1960
XapI	RAATTY	2 cut(s)	502, 1354
XceI	RCATGY	1 cut(s)	720
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1523
XhoI	CTCGAG	1 cut(s)	1909
XmiI	GTMKAC	1 cut(s)	2286
XmnI	GAANNNNTTC	2 cut(s)	253, 1560
XspI	CTAG	3 cut(s)	354, 1047, 1635
Zsp2I	ATGCAT	1 cut(s)	1905

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.