AT4G22260

Belongs to the alternative oxidase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0014010
Physical Location & Seq
Reverse (-)
11769668 .. 11772599
2932 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G22260.1

Sequence Viewer

Length: 1056 bp
ATGGCGGCGATTTCAGGCATCTCCTCTGGTACGTTGACGATTTCACGGCCTTTGGTTACTCTTCGACGCTCTAGAGCCGCCGTTTCGTACAGCTCCTCTCACCGATTGCTTCATCATCTTCCTCTCTCTTCTCGTCGTCTGCTATTAAGGAACAATCATCGAGTCCAAGCAACGATTTTGCAAGACGATGAAGAGAAAGTGGTGGTGGAGGAATCGTTTAAAGCCGAGACTTCTACTGGTACAGAACCACTTGAGGAGCCAAATATGAGTTCTTCTTCAACTAGTGCTTTTGAGACATGGATCATCAAGCTTGAGCAAGGAGTGAATGTTTTCCTTACAGACTCGGTTATTAAGATACTTGACACTTTGTATCGTGACCGAACATATGCAAGGTTCTTTGTTCTTGAGACAATTGCTAGAGTGCCTTATTTTGCGTTTATGTCTGTGCTACATATGTATGAGACCTTTGGTTGGTGGAGGAGAGCAGATTATTTGAAAGTACACTTTGCTGAGAGCTGGAATGAAATGCATCACTTGCTCATAATGGAAGAATTGGGTGGAAATTCTTGGTGGTTTGATCGTTTTCTGGCTCAGCACATAGCAACCTTCTACTACTTCATGACAGTGTTCTTGTATATCTTAAGCCCTAGAATGGCATATCACTTTTCGGAATGTGTGGAGAGTCATGCATATGAGACTTATGATAAATTTCTCAAGGCCAGTGGAGAGGAGTTGAAGAATATGCCTGCACCGGATATCGCAGTAAAATACTATACGGGAGGTGACTTGTACTTATTTGATGAGTTCCAAACATCAAGAACTCCCAATACTCGAAGACCAGTAATAGAAAATCTATACGATGTGTTTGTGAACATAAGAGATGATGAAGCAGAACACTGCAAGACAATGAGAGCTTGTCAGACTCTAGGCAGTCTGCGTTCTCCACACTCCATTTTAGAAGATGATGATACTGAAGAAGAATCAGGGTGTGTTGTTCCTGAGGAGGCTCATTGCGAAGGTATTGTAGACTGCCTCAAGAAATCCATTACAAGTTAA

Protein Analysis

351

Amino Acids

40.57

Weight (kDa)

5.41

Isoelectric Point (pI)

60.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AOX PF01786 121 - 307 3.7e-43 Alternative oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014010)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G22260
fragaria_vesca FvH4_3g01250 FvH4_3g01250 FvH4_3g01250
malus_domestica MD05G1354300.v1.1 MD10G1328600.v1.1
prunus_persica Prupe.4G013400_v2.0.a1
pyrus_communis pycom10g27840
rosa_chinensis RchiOBHm_Chr5g0001781
rosa_laevigata RLG00000030972
rosa_multiflora Rmu_ssc0000237.1_g000013
rosa_roxburghii Rroxscaffold_1G00071240 Rroxscaffold_1G00074550
rosa_rugosa Rorug04G0393000
rosa_samantha Rh5BG017700 Rh5DG015800
rosa_wichuraiana Rw5G001380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1026
AciI CCGC 2 cut(s) 5, 78
AclWI GGATC 1 cut(s) 308
AcsI RAATTY 2 cut(s) 562, 707
AcuI CTGAAG 1 cut(s) 993
AfaI GTAC 5 cut(s) 31, 89, 241, 501, 791
AfiI CCNNNNNNNGG 2 cut(s) 471, 652
AflII CTTAAG 1 cut(s) 640
AgsI TTSAA 3 cut(s) 279, 496, 736
AhlI ACTAGT 1 cut(s) 281
AjuI GAANNNNNNNTTGG 2 cut(s) 253, 285
AluBI AGCT 4 cut(s) 93, 310, 516, 914
AluI AGCT 4 cut(s) 93, 310, 516, 914
Alw26I GTCTC 5 cut(s) 221, 287, 401, 455, 689
AlwI GGATC 1 cut(s) 308
AoxI GGCC 2 cut(s) 47, 717
ApoI RAATTY 2 cut(s) 562, 707
Asp700I GAANNNNTTC 1 cut(s) 329
AsuHPI GGTGA 2 cut(s) 92, 794
AxyI CCTNAGG 1 cut(s) 999
BbsI GAAGAC 1 cut(s) 841
BceAI ACGGC 2 cut(s) 62, 65
BcoDI GTCTC 5 cut(s) 221, 287, 401, 455, 689
BcuI ACTAGT 1 cut(s) 281
BfaI CTAG 5 cut(s) 72, 282, 417, 648, 926
BfrI CTTAAG 1 cut(s) 640
BisI GCNGC 2 cut(s) 6, 78
BlpI GCTNAGC 1 cut(s) 591
BlsI GCNGC 2 cut(s) 7, 79
BmiI GGNNCC 1 cut(s) 258
BmsI GCATC 2 cut(s) 27, 538
BpiI GAAGAC 1 cut(s) 841
Bpu1102I GCTNAGC 1 cut(s) 591
BpuEI CTTGAG 5 cut(s) 272, 332, 425, 698, 1019
BsaI GGTCTC 1 cut(s) 455
BsaWI WCCGGW 1 cut(s) 751
BsaXI ACNNNNNCTCC 4 cut(s) 200, 230, 312, 342
Bsc4I CCNNNNNNNGG 2 cut(s) 471, 652
Bse1I ACTGG 3 cut(s) 241, 720, 839
Bse21I CCTNAGG 1 cut(s) 999
Bse3DI GCAATG 1 cut(s) 1009
BseLI CCNNNNNNNGG 2 cut(s) 471, 652
BseMI GCAATG 1 cut(s) 1009
BseMII CTCAG 3 cut(s) 501, 605, 990
BseNI ACTGG 3 cut(s) 241, 720, 839
BseRI GAGGAG 6 cut(s) 13, 85, 269, 493, 743, 1016
BsgI GTGCAG 1 cut(s) 732
BshFI GGCC 2 cut(s) 49, 719
BsiSI CCGG 1 cut(s) 752
BslI CCNNNNNNNGG 2 cut(s) 471, 652
BsmAI GTCTC 5 cut(s) 221, 287, 401, 455, 689
BsnI GGCC 2 cut(s) 49, 719
Bso31I GGTCTC 1 cut(s) 455
Bsp143I GATC 2 cut(s) 300, 577
Bsp1720I GCTNAGC 1 cut(s) 591
BspACI CCGC 2 cut(s) 5, 78
BspANI GGCC 2 cut(s) 49, 719
BspCNI CTCAG 3 cut(s) 502, 604, 991
BspHI TCATGA 1 cut(s) 618
BspLI GGNNCC 1 cut(s) 258
BspPI GGATC 1 cut(s) 308
BspTI CTTAAG 1 cut(s) 640
BspTNI GGTCTC 1 cut(s) 455
BsrDI GCAATG 1 cut(s) 1009
BsrI ACTGG 3 cut(s) 241, 720, 839
BssMI GATC 2 cut(s) 300, 577
Bst4CI ACNGT 1 cut(s) 625
Bst6I CTCTTC 3 cut(s) 66, 133, 186
BstAFI CTTAAG 1 cut(s) 640
BstAPI GCANNNNNTGC 1 cut(s) 535
BstC8I GCNNGC 1 cut(s) 747
BstDEI CTNAG 3 cut(s) 510, 591, 999
BstKTI GATC 2 cut(s) 303, 580
BstMAI GTCTC 5 cut(s) 221, 287, 401, 455, 689
BstMBI GATC 2 cut(s) 300, 577
BstMWI GCNNNNNNNGC 1 cut(s) 535
BstV2I GAAGAC 1 cut(s) 841
Bsu36I CCTNAGG 1 cut(s) 999
BsuRI GGCC 2 cut(s) 49, 719
BtsI GCAGTG 1 cut(s) 895
BtsIMutI CAGTG 3 cut(s) 630, 727, 895
Cac8I GCNNGC 1 cut(s) 747
CciI TCATGA 1 cut(s) 618
CseI GACGC 1 cut(s) 75
Csp6I GTAC 5 cut(s) 30, 88, 240, 500, 790
CspCI CAANNNNNGTGG 2 cut(s) 703, 738
CviAII CATG 3 cut(s) 297, 619, 686
CviQI GTAC 5 cut(s) 30, 88, 240, 500, 790
DdeI CTNAG 3 cut(s) 510, 591, 999
DpnI GATC 2 cut(s) 302, 579
DpnII GATC 2 cut(s) 300, 577
DraI TTTAAA 1 cut(s) 220
Eam1104I CTCTTC 3 cut(s) 66, 133, 186
EarI CTCTTC 3 cut(s) 66, 133, 186
Eco31I GGTCTC 1 cut(s) 455
Eco32I GATATC 1 cut(s) 757
Eco57I CTGAAG 1 cut(s) 993
Eco81I CCTNAGG 1 cut(s) 999
EcoRV GATATC 1 cut(s) 757
EcoT22I ATGCAT 2 cut(s) 531, 691
FaeI CATG 3 cut(s) 300, 622, 689
FatI CATG 3 cut(s) 296, 618, 685
FauNDI CATATG 3 cut(s) 385, 453, 691
FblI GTMKAC 1 cut(s) 1026
Fnu4HI GCNGC 2 cut(s) 6, 78
Fsp4HI GCNGC 2 cut(s) 6, 78
FspBI CTAG 5 cut(s) 72, 282, 417, 648, 926
GluI GCNGC 2 cut(s) 6, 78
HaeIII GGCC 2 cut(s) 49, 719
HapII CCGG 1 cut(s) 752
HgaI GACGC 1 cut(s) 75
Hin1II CATG 3 cut(s) 300, 622, 689
HincII GTYRAC 1 cut(s) 36
HindII GTYRAC 1 cut(s) 36
HindIII AAGCTT 1 cut(s) 308
HinfI GANTC 6 cut(s) 162, 212, 341, 682, 922, 980
HpaII CCGG 1 cut(s) 752
HphI GGTGA 2 cut(s) 92, 794
Hpy166II GTNNAC 4 cut(s) 36, 502, 871, 1027
Hpy188I TCNGA 2 cut(s) 670, 921
Hpy188III TCNNGA 7 cut(s) 72, 374, 404, 619, 816, 998, 1036
Hpy8I GTNNAC 4 cut(s) 36, 502, 871, 1027
Hpy99I CGWCG 2 cut(s) 69, 138
HpyAV CCTTC 2 cut(s) 616, 1010
HpyCH4III ACNGT 1 cut(s) 625
HpyCH4IV ACGT 1 cut(s) 32
HpyCH4V TGCA 6 cut(s) 181, 389, 529, 689, 749, 900
HpyF10VI GCNNNNNNNGC 1 cut(s) 535
HpyF3I CTNAG 3 cut(s) 510, 591, 999
HpySE526I ACGT 1 cut(s) 32
Hsp92II CATG 3 cut(s) 300, 622, 689
Kzo9I GATC 2 cut(s) 300, 577
LmnI GCTCC 2 cut(s) 98, 256
LweI GCATC 2 cut(s) 27, 538
MaeI CTAG 5 cut(s) 72, 282, 417, 648, 926
MaeII ACGT 1 cut(s) 32
MaeIII GTNAC 3 cut(s) 55, 374, 782
MalI GATC 2 cut(s) 302, 579
MboI GATC 2 cut(s) 300, 577
MfeI CAATTG 1 cut(s) 411
MluCI AATT 4 cut(s) 411, 551, 562, 707
MlyI GAGTC 4 cut(s) 171, 335, 691, 916
Mph1103I ATGCAT 2 cut(s) 531, 691
MroXI GAANNNNTTC 1 cut(s) 329
MseI TTAA 5 cut(s) 146, 219, 351, 641, 1054
MslI CAYNNNNRTG 3 cut(s) 456, 623, 690
MspCI CTTAAG 1 cut(s) 640
MspI CCGG 1 cut(s) 752
MunI CAATTG 1 cut(s) 411
MwoI GCNNNNNNNGC 1 cut(s) 535
NdeI CATATG 3 cut(s) 385, 453, 691
NdeII GATC 2 cut(s) 300, 577
NlaIII CATG 3 cut(s) 300, 622, 689
NlaIV GGNNCC 1 cut(s) 258
NmeAIII GCCGAG 1 cut(s) 250
NmuCI GTSAC 2 cut(s) 374, 782
NsiI ATGCAT 2 cut(s) 531, 691
PagI TCATGA 1 cut(s) 618
PdmI GAANNNNTTC 1 cut(s) 329
PfeI GAWTC 2 cut(s) 212, 980
PkrI GCNGC 2 cut(s) 7, 79
PleI GAGTC 4 cut(s) 170, 335, 690, 916
PpsI GAGTC 4 cut(s) 170, 335, 690, 916
PspN4I GGNNCC 1 cut(s) 258
PsrI GAACNNNNNNTAC 2 cut(s) 811, 843
RsaI GTAC 5 cut(s) 31, 89, 241, 501, 791
RsaNI GTAC 5 cut(s) 30, 88, 240, 500, 790
RseI CAYNNNNRTG 3 cut(s) 456, 623, 690
SaqAI TTAA 5 cut(s) 146, 219, 351, 641, 1054
SatI GCNGC 2 cut(s) 6, 78
Sau3AI GATC 2 cut(s) 300, 577
SchI GAGTC 4 cut(s) 171, 335, 691, 916
SfaNI GCATC 2 cut(s) 27, 538
SmiMI CAYNNNNRTG 3 cut(s) 456, 623, 690
SmlI CTYRAG 6 cut(s) 251, 311, 404, 640, 713, 1034
SmoI CTYRAG 6 cut(s) 251, 311, 404, 640, 713, 1034
SpeI ACTAGT 1 cut(s) 281
Sse9I AATT 4 cut(s) 411, 551, 562, 707
SsiI CCGC 2 cut(s) 5, 78
SspMI CTAG 5 cut(s) 72, 282, 417, 648, 926
TaaI ACNGT 1 cut(s) 625
TaiI ACGT 1 cut(s) 35
TaqI TCGA 3 cut(s) 64, 160, 832
TaqII GACCGA 1 cut(s) 393
TasI AATT 4 cut(s) 411, 551, 562, 707
TatI WGTACW 2 cut(s) 499, 789
TauI GCSGC 2 cut(s) 8, 80
TfiI GAWTC 2 cut(s) 212, 980
Tru1I TTAA 5 cut(s) 146, 219, 351, 641, 1054
Tru9I TTAA 5 cut(s) 146, 219, 351, 641, 1054
TscAI CASTG 3 cut(s) 630, 727, 902
TseFI GTSAC 2 cut(s) 374, 782
Tsp45I GTSAC 2 cut(s) 374, 782
TspDTI ATGAA 5 cut(s) 101, 204, 537, 607, 900
TspRI CASTG 3 cut(s) 630, 727, 902
Vha464I CTTAAG 1 cut(s) 640
XapI RAATTY 2 cut(s) 562, 707
XbaI TCTAGA 1 cut(s) 71
XmiI GTMKAC 1 cut(s) 1026
XmnI GAANNNNTTC 1 cut(s) 329
XspI CTAG 5 cut(s) 72, 282, 417, 648, 926
Zsp2I ATGCAT 2 cut(s) 531, 691
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.