Rw5G001380

Belongs to the alternative oxidase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0014010
Physical Location & Seq
Forward (+)
1126958 .. 1130774
3817 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G001380.1

Sequence Viewer

Length: 1110 bp
ATGGCAGCGGCGACTCTGTCTTCGACGGTGTCTGCAATCCCAACTTCCTGCTCTTCTTCTTCTTCTTCTTCGTCTCTCAAAGCAAGGACCTTGAAGACTTCAGCTTTTTCTTCTTCGTCCTCGCTCTGCTCTCTGAATCGTTTTCCTTGCAATCCCATCTCAGCTCGTCCATCGTGTTCCAGAAATTTGTATAGGGTACATGCAACTATTCTCCAAGATGAAGAAGAGAAAGTGGTGGTGGAGGAGTCCTTTGAGTTCAAGGCAACTACCTCTGATGAAGTGAAAGCCAGCAGTGGGGAGGCCTCTGAGAGTGAGCCTTCTAGTGATTTTGAGAAATGGGTTATCAAGGGTGAACAATCCATCAACGTTTTCCTTACGGATACAGTGATTAAGGTACTTGATACTCTTTACCGCGACCGTAATTATGCCAGGTTCTTTGTACTGGAAACTATTGCAAGGGTTCCCTACTTCGCCTTTATGTCTGTTCTGCACTTGTATGAGACTTTTGGCTGGTGGAGGAGAAGAGATTATTTGAAAGTGCATTTTGCTGAGAGCTGGAATGAGATGCACCACTTGCTTATCATGGAAGAATTGGGAGGAAATGCTTGGTGGTTTGATCGCTTTCTTGCTCAGCACATTGCGGTCTTCTATTACTTTATGACAGTTTTTATGTATTTAATAAGCCCACGAATGGCATATCACTTCTCTGAATGTGTAGAGGGCCATGCATTTTCGACATATGACAAGTTTATCAAGGCTGAAGGAGAGAATCTGAAAAAATTGCCTGCTCCTGAGGTTGCTGTAAAATACTACACTGGAGGTGACTTGTACTTGTTTGATGAATTTCAAACTTCCAGACCTCCCAATTCTCGAAGGCCGAAAATAGAGAATTTGTTCGATGTATTTCTGAACATAAGAGATGATGAAGCTGAGCACTGTAAGACGATGAAGGCCTGCCAAACTGACGGGAACCTCTTTTCTCCTCATTCAATTGTAGAAGATCCCTTGGATCCTGTGGAAGATGAGTCTATCTGTGTCGCTCCTCAAACAGATTCTGAAGGTTTTGTAGATTCTATTACAAAATCCGTTGCAACGAAACCAGCAGAGTGA

Protein Analysis

369

Amino Acids

41.98

Weight (kDa)

5.2

Isoelectric Point (pI)

55.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AOX PF01786 125 - 320 2.4e-42 Alternative oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014010)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G22260
fragaria_vesca FvH4_3g01250 FvH4_3g01250 FvH4_3g01250
malus_domestica MD05G1354300.v1.1 MD10G1328600.v1.1
prunus_persica Prupe.4G013400_v2.0.a1
pyrus_communis pycom10g27840
rosa_chinensis RchiOBHm_Chr5g0001781
rosa_laevigata RLG00000030972
rosa_multiflora Rmu_ssc0000237.1_g000013
rosa_roxburghii Rroxscaffold_1G00071240 Rroxscaffold_1G00074550
rosa_rugosa Rorug04G0393000
rosa_samantha Rh5BG017700 Rh5DG015800
rosa_wichuraiana Rw5G001380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 414
AciI CCGC 3 cut(s) 8, 412, 641
AclI AACGTT 1 cut(s) 366
AclWI GGATC 3 cut(s) 995, 1004, 1017
AcsI RAATTY 3 cut(s) 184, 842, 889
AcuI CTGAAG 3 cut(s) 84, 780, 1077
AfaI GTAC 4 cut(s) 198, 396, 441, 830
AfiI CCNNNNNNNGG 2 cut(s) 294, 691
AgsI TTSAA 5 cut(s) 94, 259, 535, 848, 990
AjnI CCWGG 1 cut(s) 428
AluBI AGCT 4 cut(s) 104, 164, 555, 929
AluI AGCT 4 cut(s) 104, 164, 555, 929
Alw21I GWGCWC 1 cut(s) 936
Alw26I GTCTC 2 cut(s) 78, 494
AlwI GGATC 3 cut(s) 995, 1004, 1017
AlwNI CAGNNNCTG 1 cut(s) 1055
AoxI GGCC 4 cut(s) 300, 721, 875, 951
ApeKI GCWGC 1 cut(s) 5
ApoI RAATTY 3 cut(s) 184, 842, 889
ArsI GACNNNNNNTTYG 1 cut(s) 36
Asp700I GAANNNNTTC 1 cut(s) 893
AspS9I GGNCC 2 cut(s) 87, 721
AsuHPI GGTGA 2 cut(s) 362, 833
AvaII GGWCC 1 cut(s) 87
AxyI CCTNAGG 1 cut(s) 792
BamHI GGATCC 1 cut(s) 1009
BbsI GAAGAC 3 cut(s) 12, 101, 637
Bbv12I GWGCWC 1 cut(s) 936
BbvI GCAGC 1 cut(s) 17
BccI CCATC 3 cut(s) 164, 178, 368
BciT130I CCWGG 1 cut(s) 430
BciVI GTATCC 1 cut(s) 373
BcoDI GTCTC 2 cut(s) 78, 494
BfaI CTAG 1 cut(s) 321
BfuI GTATCC 1 cut(s) 373
BisI GCNGC 2 cut(s) 6, 9
BlpI GCTNAGC 2 cut(s) 630, 930
BlsI GCNGC 2 cut(s) 7, 10
Bme1390I CCNGG 1 cut(s) 430
Bme18I GGWCC 1 cut(s) 87
BmgT120I GGNCC 2 cut(s) 87, 721
BmiI GGNNCC 3 cut(s) 462, 971, 1011
BmrFI CCNGG 1 cut(s) 430
BmsI GCATC 1 cut(s) 555
BpiI GAAGAC 3 cut(s) 12, 101, 637
BpmI CTGGAG 1 cut(s) 837
Bpu1102I GCTNAGC 2 cut(s) 630, 930
BsaJI CCNNGG 1 cut(s) 1005
Bsc4I CCNNNNNNNGG 2 cut(s) 294, 691
Bse1I ACTGG 2 cut(s) 447, 820
Bse21I CCTNAGG 1 cut(s) 792
Bse3DI GCAATG 1 cut(s) 636
BseBI CCWGG 1 cut(s) 430
BseDI CCNNGG 1 cut(s) 1005
BseLI CCNNNNNNNGG 2 cut(s) 294, 691
BseMI GCAATG 1 cut(s) 636
BseMII CTCAG 6 cut(s) 174, 297, 540, 644, 783, 921
BseNI ACTGG 2 cut(s) 447, 820
BseRI GAGGAG 4 cut(s) 257, 532, 972, 1032
BseXI GCAGC 1 cut(s) 17
BsgI GTGCAG 1 cut(s) 473
Bsh1236I CGCG 1 cut(s) 414
Bsh1285I CGRYCG 1 cut(s) 418
BshFI GGCC 4 cut(s) 302, 723, 877, 953
BsiEI CGRYCG 1 cut(s) 418
BsiHKAI GWGCWC 1 cut(s) 936
BslI CCNNNNNNNGG 2 cut(s) 294, 691
BsmAI GTCTC 2 cut(s) 78, 494
BsmBI CGTCTC 1 cut(s) 78
BsnI GGCC 4 cut(s) 302, 723, 877, 953
Bsp1286I GDGCHC 1 cut(s) 936
Bsp143I GATC 3 cut(s) 616, 1000, 1009
Bsp1720I GCTNAGC 2 cut(s) 630, 930
BspACI CCGC 3 cut(s) 8, 412, 641
BspANI GGCC 4 cut(s) 302, 723, 877, 953
BspCNI CTCAG 6 cut(s) 173, 298, 541, 643, 784, 922
BspFNI CGCG 1 cut(s) 414
BspLI GGNNCC 3 cut(s) 462, 971, 1011
BspPI GGATC 3 cut(s) 995, 1004, 1017
BspQI GCTCTTC 1 cut(s) 58
BsrDI GCAATG 1 cut(s) 636
BsrI ACTGG 2 cut(s) 447, 820
BssECI CCNNGG 1 cut(s) 1005
BssMI GATC 3 cut(s) 616, 1000, 1009
BssT1I CCWWGG 1 cut(s) 1005
Bst2UI CCWGG 1 cut(s) 430
Bst4CI ACNGT 5 cut(s) 28, 385, 419, 664, 938
Bst6I CTCTTC 3 cut(s) 58, 219, 517
BstAPI GCANNNNNTGC 1 cut(s) 574
BstC8I GCNNGC 3 cut(s) 289, 786, 955
BstDEI CTNAG 6 cut(s) 160, 306, 549, 630, 792, 930
BstFNI CGCG 1 cut(s) 414
BstKTI GATC 3 cut(s) 619, 1003, 1012
BstMAI GTCTC 2 cut(s) 78, 494
BstMBI GATC 3 cut(s) 616, 1000, 1009
BstMCI CGRYCG 1 cut(s) 418
BstMWI GCNNNNNNNGC 1 cut(s) 574
BstNI CCWGG 1 cut(s) 430
BstNSI RCATGY 1 cut(s) 203
BstSCI CCNGG 1 cut(s) 428
BstUI CGCG 1 cut(s) 414
BstV1I GCAGC 1 cut(s) 17
BstV2I GAAGAC 3 cut(s) 12, 101, 637
BstX2I RGATCY 2 cut(s) 1000, 1009
BstYI RGATCY 2 cut(s) 1000, 1009
Bsu36I CCTNAGG 1 cut(s) 792
BsuI GTATCC 1 cut(s) 373
BsuRI GGCC 4 cut(s) 302, 723, 877, 953
BtsI GCAGTG 1 cut(s) 298
BtsIMutI CAGTG 4 cut(s) 298, 390, 813, 934
Cac8I GCNNGC 3 cut(s) 289, 786, 955
CaiI CAGNNNCTG 1 cut(s) 1055
Cfr13I GGNCC 2 cut(s) 87, 721
Csp6I GTAC 4 cut(s) 197, 395, 440, 829
CviAII CATG 3 cut(s) 200, 583, 725
CviQI GTAC 4 cut(s) 197, 395, 440, 829
DdeI CTNAG 6 cut(s) 160, 306, 549, 630, 792, 930
DpnI GATC 3 cut(s) 618, 1002, 1011
DpnII GATC 3 cut(s) 616, 1000, 1009
Eam1104I CTCTTC 3 cut(s) 58, 219, 517
EarI CTCTTC 3 cut(s) 58, 219, 517
Eco130I CCWWGG 1 cut(s) 1005
Eco147I AGGCCT 2 cut(s) 302, 953
Eco47I GGWCC 1 cut(s) 87
Eco57I CTGAAG 3 cut(s) 84, 780, 1077
Eco81I CCTNAGG 1 cut(s) 792
EcoO109I RGGNCCY 1 cut(s) 87
EcoRII CCWGG 1 cut(s) 428
EcoT14I CCWWGG 1 cut(s) 1005
EcoT22I ATGCAT 1 cut(s) 730
ErhI CCWWGG 1 cut(s) 1005
Esp3I CGTCTC 1 cut(s) 78
FaeI CATG 3 cut(s) 203, 586, 728
FatI CATG 3 cut(s) 199, 582, 724
FauNDI CATATG 1 cut(s) 739
Fnu4HI GCNGC 2 cut(s) 6, 9
Fsp4HI GCNGC 2 cut(s) 6, 9
FspBI CTAG 1 cut(s) 321
GluI GCNGC 2 cut(s) 6, 9
GsuI CTGGAG 1 cut(s) 837
HaeIII GGCC 4 cut(s) 302, 723, 877, 953
Hin1II CATG 3 cut(s) 203, 586, 728
HinfI GANTC 7 cut(s) 13, 136, 245, 769, 1025, 1052, 1070
HphI GGTGA 2 cut(s) 362, 833
Hpy166II GTNNAC 1 cut(s) 353
Hpy188I TCNGA 7 cut(s) 135, 274, 307, 709, 774, 909, 1057
Hpy188III TCNNGA 4 cut(s) 180, 791, 855, 870
Hpy8I GTNNAC 1 cut(s) 353
Hpy99I CGWCG 1 cut(s) 28
HpyAV CCTTC 5 cut(s) 327, 755, 867, 943, 1052
HpyCH4III ACNGT 5 cut(s) 28, 385, 419, 664, 938
HpyCH4IV ACGT 1 cut(s) 366
HpyCH4V TGCA 9 cut(s) 35, 150, 203, 455, 490, 541, 568, 728, 1091
HpyF10VI GCNNNNNNNGC 1 cut(s) 574
HpyF3I CTNAG 6 cut(s) 160, 306, 549, 630, 792, 930
HpySE526I ACGT 1 cut(s) 366
Hsp92II CATG 3 cut(s) 203, 586, 728
Kzo9I GATC 3 cut(s) 616, 1000, 1009
LguI GCTCTTC 1 cut(s) 58
LmnI GCTCC 2 cut(s) 793, 1045
Lsp1109I GCAGC 1 cut(s) 17
LweI GCATC 1 cut(s) 555
MaeI CTAG 1 cut(s) 321
MaeII ACGT 1 cut(s) 366
MaeIII GTNAC 1 cut(s) 821
MalI GATC 3 cut(s) 618, 1002, 1011
MboI GATC 3 cut(s) 616, 1000, 1009
MfeI CAATTG 1 cut(s) 990
MflI RGATCY 2 cut(s) 1000, 1009
MhlI GDGCHC 1 cut(s) 936
MluCI AATT 8 cut(s) 184, 421, 590, 779, 842, 865, 889, 990
MlyI GAGTC 3 cut(s) 7, 254, 1034
Mph1103I ATGCAT 1 cut(s) 730
MroXI GAANNNNTTC 1 cut(s) 893
MseI TTAA 2 cut(s) 390, 677
MslI CAYNNNNRTG 1 cut(s) 495
MspA1I CMGCKG 1 cut(s) 8
MspR9I CCNGG 1 cut(s) 430
MunI CAATTG 1 cut(s) 990
MvaI CCWGG 1 cut(s) 430
MvnI CGCG 1 cut(s) 414
MwoI GCNNNNNNNGC 1 cut(s) 574
NdeI CATATG 1 cut(s) 739
NdeII GATC 3 cut(s) 616, 1000, 1009
NlaIII CATG 3 cut(s) 203, 586, 728
NlaIV GGNNCC 3 cut(s) 462, 971, 1011
NmuCI GTSAC 1 cut(s) 821
NsiI ATGCAT 1 cut(s) 730
NspI RCATGY 1 cut(s) 203
PceI AGGCCT 2 cut(s) 302, 953
PciSI GCTCTTC 1 cut(s) 58
PdmI GAANNNNTTC 1 cut(s) 893
PfeI GAWTC 4 cut(s) 136, 769, 1052, 1070
PflFI GACNNNGTC 2 cut(s) 16, 28
PkrI GCNGC 2 cut(s) 7, 10
PleI GAGTC 3 cut(s) 7, 253, 1033
PpsI GAGTC 3 cut(s) 7, 253, 1033
PpuMI RGGWCCY 1 cut(s) 87
Psp1406I AACGTT 1 cut(s) 366
Psp5II RGGWCCY 1 cut(s) 87
Psp6I CCWGG 1 cut(s) 428
PspGI CCWGG 1 cut(s) 428
PspN4I GGNNCC 3 cut(s) 462, 971, 1011
PspPI GGNCC 2 cut(s) 87, 721
PspPPI RGGWCCY 1 cut(s) 87
PstNI CAGNNNCTG 1 cut(s) 1055
PsuI RGATCY 2 cut(s) 1000, 1009
PsyI GACNNNGTC 2 cut(s) 16, 28
RsaI GTAC 4 cut(s) 198, 396, 441, 830
RsaNI GTAC 4 cut(s) 197, 395, 440, 829
RseI CAYNNNNRTG 1 cut(s) 495
SapI GCTCTTC 1 cut(s) 58
SaqAI TTAA 2 cut(s) 390, 677
SatI GCNGC 2 cut(s) 6, 9
Sau3AI GATC 3 cut(s) 616, 1000, 1009
Sau96I GGNCC 2 cut(s) 87, 721
SchI GAGTC 3 cut(s) 7, 254, 1034
ScrFI CCNGG 1 cut(s) 430
SduI GDGCHC 1 cut(s) 936
SfaNI GCATC 1 cut(s) 555
SinI GGWCC 1 cut(s) 87
SmiMI CAYNNNNRTG 1 cut(s) 495
Sse9I AATT 8 cut(s) 184, 421, 590, 779, 842, 865, 889, 990
SseBI AGGCCT 2 cut(s) 302, 953
SsiI CCGC 3 cut(s) 8, 412, 641
SspMI CTAG 1 cut(s) 321
StuI AGGCCT 2 cut(s) 302, 953
StyD4I CCNGG 1 cut(s) 428
StyI CCWWGG 1 cut(s) 1005
TaaI ACNGT 5 cut(s) 28, 385, 419, 664, 938
TaiI ACGT 1 cut(s) 369
TaqI TCGA 4 cut(s) 23, 734, 871, 897
TasI AATT 8 cut(s) 184, 421, 590, 779, 842, 865, 889, 990
TatI WGTACW 2 cut(s) 439, 828
TauI GCSGC 1 cut(s) 11
TfiI GAWTC 4 cut(s) 136, 769, 1052, 1070
Tru1I TTAA 2 cut(s) 390, 677
Tru9I TTAA 2 cut(s) 390, 677
TscAI CASTG 4 cut(s) 298, 390, 820, 941
TseFI GTSAC 1 cut(s) 821
TseI GCWGC 1 cut(s) 5
Tsp45I GTSAC 1 cut(s) 821
TspDTI ATGAA 5 cut(s) 234, 291, 855, 939, 962
TspGWI ACGGA 2 cut(s) 392, 1075
TspRI CASTG 4 cut(s) 298, 390, 820, 941
Tth111I GACNNNGTC 2 cut(s) 16, 28
VpaK11BI GGWCC 1 cut(s) 87
XapI RAATTY 3 cut(s) 184, 842, 889
XceI RCATGY 1 cut(s) 203
XmnI GAANNNNTTC 1 cut(s) 893
XspI CTAG 1 cut(s) 321
Zsp2I ATGCAT 1 cut(s) 730
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.