Rh5DG015800

Belongs to the alternative oxidase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0014010
Physical Location & Seq
Forward (+)
1189742 .. 1192998
3257 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG015800.1

Sequence Viewer

Length: 1128 bp
ATGGCAGCGGCGACTCTGTCTTCGACGGTGTTTGCAATCCCAACTTCCTGCTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCGTCTCTCAAAGCAAGGACCTTGAAGACTTCAGCTTTTTCTTCTTCGTCCTCGCTCTGCTCTCTGAATCGTTTTCCTTGCAATCCCATCTCAGCTCGTCCATCGTGTTCCAGAAATTTGTATAGGGTGCATGCAACTATTCTCCAAGATGAAGAAGAGAAAGTGGTGGTGGAGGAGTCCTTTGAGTTCAAGGCAACTACGTCTGATGAAGTGAAAGCCAGCAGTGGGGAGGCCTCTGAGAGTGAGCCTTCTAGTGATTTTGAGAAATGGGTTATCAAGGGTGAACAATCCATCAACGTTTTCCTTACGGATACAGTGATTAAGGTACTTGATACTCTTTACCACGACCGTGATTATGCGAGGTTCTTTGTACTGGAAACTATTGCAAGGGTTCCCTACTTCGCCTTTATGTCTGTTCTACACATGTATGAGACTTTTGGGTGGTGGAGAAGAAGAGATTATTTGAAAGTGCATTTTGCTGAGAGCTGGAATGAGATGCACCACTTGCTTATCATGGAAGAATTGGGAGGAAATGCTTGGTGGTTTGATCGCTTTCTTGCTCAGCACATTGCGGTCTTCTATTACTTTATGACAGTTTTTATGTATTTAATAAGCCCACGAATGGCATATCACTTCTCTGAATGTGTAGAGGGCCATGCATTTTCAACATATGACAAGTTTATCAAGGCTGAAGGAGAGAATCTGAAAAAATTGCCTGCTCCTGAGGTTGCTGTAAAATACTACACTGGAGGTGACTTGTACTTGTTTGATGAATTTCAAACTTCCAGATCTCCCAATTCTCGAAGGCCAAAAATAGAGAATTTGTTCGATGTATTTCTGAACATAAGAGATGATGAAGCTGAGCACTGTAAGACGATGAAGGCCTGCCAAACTCACGGGAACCTCTTTTCTCCTCATTCAATTGTAGAAGATCCCTTGGATCCCTTGGAAGATGAGTCTTTCTGTGTCGCTCCTCAAACAGATTGTGAAGGTATTGTAGATTGTATTACAAAATCCGTTGCAACGAAACCAGCAGAGTGA

Protein Analysis

375

Amino Acids

42.62

Weight (kDa)

5.24

Isoelectric Point (pI)

57.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AOX PF01786 130 - 326 7.6e-42 Alternative oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014010)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G22260
fragaria_vesca FvH4_3g01250 FvH4_3g01250 FvH4_3g01250
malus_domestica MD05G1354300.v1.1 MD10G1328600.v1.1
prunus_persica Prupe.4G013400_v2.0.a1
pyrus_communis pycom10g27840
rosa_chinensis RchiOBHm_Chr5g0001781
rosa_laevigata RLG00000030972
rosa_multiflora Rmu_ssc0000237.1_g000013
rosa_roxburghii Rroxscaffold_1G00071240 Rroxscaffold_1G00074550
rosa_rugosa Rorug04G0393000
rosa_samantha Rh5BG017700 Rh5DG015800
rosa_wichuraiana Rw5G001380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 8, 659
AclI AACGTT 1 cut(s) 384
AclWI GGATC 3 cut(s) 1013, 1022, 1035
AcsI RAATTY 3 cut(s) 202, 860, 907
AcuI CTGAAG 2 cut(s) 102, 798
AfaI GTAC 3 cut(s) 414, 459, 848
AfiI CCNNNNNNNGG 2 cut(s) 312, 709
AflIII ACRYGT 1 cut(s) 510
AgsI TTSAA 6 cut(s) 112, 277, 553, 753, 866, 1008
AleI CACNNNNGTG 1 cut(s) 435
AluBI AGCT 4 cut(s) 122, 182, 573, 947
AluI AGCT 4 cut(s) 122, 182, 573, 947
Alw21I GWGCWC 1 cut(s) 954
Alw26I GTCTC 2 cut(s) 96, 512
AlwI GGATC 3 cut(s) 1013, 1022, 1035
AoxI GGCC 4 cut(s) 318, 739, 893, 969
ApeKI GCWGC 1 cut(s) 5
ApoI RAATTY 3 cut(s) 202, 860, 907
ArsI GACNNNNNNTTYG 1 cut(s) 36
Asp700I GAANNNNTTC 1 cut(s) 911
AspS9I GGNCC 2 cut(s) 105, 739
AsuHPI GGTGA 2 cut(s) 380, 851
AvaII GGWCC 1 cut(s) 105
AxyI CCTNAGG 1 cut(s) 810
BamHI GGATCC 1 cut(s) 1027
BbsI GAAGAC 3 cut(s) 12, 119, 655
Bbv12I GWGCWC 1 cut(s) 954
BbvI GCAGC 1 cut(s) 17
BccI CCATC 3 cut(s) 182, 196, 386
BciVI GTATCC 1 cut(s) 391
BcoDI GTCTC 2 cut(s) 96, 512
BfaI CTAG 1 cut(s) 339
BfuI GTATCC 1 cut(s) 391
BglII AGATCT 1 cut(s) 875
BisI GCNGC 2 cut(s) 6, 9
BlpI GCTNAGC 2 cut(s) 648, 948
BlsI GCNGC 2 cut(s) 7, 10
Bme18I GGWCC 1 cut(s) 105
BmgT120I GGNCC 2 cut(s) 105, 739
BmiI GGNNCC 3 cut(s) 480, 989, 1029
BmsI GCATC 1 cut(s) 573
BpiI GAAGAC 3 cut(s) 12, 119, 655
BpmI CTGGAG 1 cut(s) 855
Bpu1102I GCTNAGC 2 cut(s) 648, 948
BsaJI CCNNGG 2 cut(s) 1023, 1032
Bsc4I CCNNNNNNNGG 2 cut(s) 312, 709
Bse1I ACTGG 2 cut(s) 465, 838
Bse21I CCTNAGG 1 cut(s) 810
Bse3DI GCAATG 1 cut(s) 654
BseDI CCNNGG 2 cut(s) 1023, 1032
BseLI CCNNNNNNNGG 2 cut(s) 312, 709
BseMI GCAATG 1 cut(s) 654
BseMII CTCAG 6 cut(s) 192, 315, 558, 662, 801, 939
BseNI ACTGG 2 cut(s) 465, 838
BseRI GAGGAG 3 cut(s) 275, 990, 1050
BseXI GCAGC 1 cut(s) 17
Bsh1285I CGRYCG 1 cut(s) 436
BshFI GGCC 4 cut(s) 320, 741, 895, 971
BsiEI CGRYCG 1 cut(s) 436
BsiHKAI GWGCWC 1 cut(s) 954
BslI CCNNNNNNNGG 2 cut(s) 312, 709
BsmAI GTCTC 2 cut(s) 96, 512
BsmBI CGTCTC 1 cut(s) 96
BsnI GGCC 4 cut(s) 320, 741, 895, 971
Bsp1286I GDGCHC 1 cut(s) 954
Bsp143I GATC 4 cut(s) 634, 875, 1018, 1027
Bsp1720I GCTNAGC 2 cut(s) 648, 948
BspACI CCGC 2 cut(s) 8, 659
BspANI GGCC 4 cut(s) 320, 741, 895, 971
BspCNI CTCAG 6 cut(s) 191, 316, 559, 661, 802, 940
BspLI GGNNCC 3 cut(s) 480, 989, 1029
BspPI GGATC 3 cut(s) 1013, 1022, 1035
BspQI GCTCTTC 1 cut(s) 58
BsrDI GCAATG 1 cut(s) 654
BsrI ACTGG 2 cut(s) 465, 838
BssECI CCNNGG 2 cut(s) 1023, 1032
BssMI GATC 4 cut(s) 634, 875, 1018, 1027
BssT1I CCWWGG 2 cut(s) 1023, 1032
Bst4CI ACNGT 5 cut(s) 28, 403, 437, 682, 956
Bst6I CTCTTC 3 cut(s) 58, 237, 535
BstAPI GCANNNNNTGC 1 cut(s) 592
BstC8I GCNNGC 4 cut(s) 219, 307, 804, 973
BstDEI CTNAG 6 cut(s) 178, 324, 567, 648, 810, 948
BstKTI GATC 4 cut(s) 637, 878, 1021, 1030
BstMAI GTCTC 2 cut(s) 96, 512
BstMBI GATC 4 cut(s) 634, 875, 1018, 1027
BstMCI CGRYCG 1 cut(s) 436
BstMWI GCNNNNNNNGC 1 cut(s) 592
BstNSI RCATGY 2 cut(s) 221, 514
BstV1I GCAGC 1 cut(s) 17
BstV2I GAAGAC 3 cut(s) 12, 119, 655
BstX2I RGATCY 3 cut(s) 875, 1018, 1027
BstYI RGATCY 3 cut(s) 875, 1018, 1027
Bsu36I CCTNAGG 1 cut(s) 810
BsuI GTATCC 1 cut(s) 391
BsuRI GGCC 4 cut(s) 320, 741, 895, 971
BtsI GCAGTG 1 cut(s) 316
BtsIMutI CAGTG 4 cut(s) 316, 408, 831, 952
Cac8I GCNNGC 4 cut(s) 219, 307, 804, 973
Cfr13I GGNCC 2 cut(s) 105, 739
Csp6I GTAC 3 cut(s) 413, 458, 847
CviAII CATG 4 cut(s) 218, 511, 601, 743
CviQI GTAC 3 cut(s) 413, 458, 847
DdeI CTNAG 6 cut(s) 178, 324, 567, 648, 810, 948
DpnI GATC 4 cut(s) 636, 877, 1020, 1029
DpnII GATC 4 cut(s) 634, 875, 1018, 1027
Eam1104I CTCTTC 3 cut(s) 58, 237, 535
EarI CTCTTC 3 cut(s) 58, 237, 535
Eco130I CCWWGG 2 cut(s) 1023, 1032
Eco147I AGGCCT 2 cut(s) 320, 971
Eco47I GGWCC 1 cut(s) 105
Eco57I CTGAAG 2 cut(s) 102, 798
Eco81I CCTNAGG 1 cut(s) 810
EcoO109I RGGNCCY 1 cut(s) 105
EcoT14I CCWWGG 2 cut(s) 1023, 1032
EcoT22I ATGCAT 1 cut(s) 748
ErhI CCWWGG 2 cut(s) 1023, 1032
Esp3I CGTCTC 1 cut(s) 96
FaeI CATG 4 cut(s) 221, 514, 604, 746
FatI CATG 4 cut(s) 217, 510, 600, 742
FauNDI CATATG 1 cut(s) 757
Fnu4HI GCNGC 2 cut(s) 6, 9
Fsp4HI GCNGC 2 cut(s) 6, 9
FspBI CTAG 1 cut(s) 339
GluI GCNGC 2 cut(s) 6, 9
GsuI CTGGAG 1 cut(s) 855
HaeIII GGCC 4 cut(s) 320, 741, 895, 971
Hin1II CATG 4 cut(s) 221, 514, 604, 746
HinfI GANTC 5 cut(s) 13, 154, 263, 787, 1043
HphI GGTGA 2 cut(s) 380, 851
Hpy166II GTNNAC 1 cut(s) 371
Hpy188I TCNGA 6 cut(s) 153, 292, 325, 727, 792, 927
Hpy188III TCNNGA 4 cut(s) 198, 809, 873, 888
Hpy8I GTNNAC 1 cut(s) 371
Hpy99I CGWCG 1 cut(s) 28
HpyAV CCTTC 5 cut(s) 345, 773, 885, 961, 1070
HpyCH4III ACNGT 5 cut(s) 28, 403, 437, 682, 956
HpyCH4IV ACGT 2 cut(s) 287, 384
HpyCH4V TGCA 9 cut(s) 35, 168, 217, 221, 473, 559, 586, 746, 1109
HpyF10VI GCNNNNNNNGC 1 cut(s) 592
HpyF3I CTNAG 6 cut(s) 178, 324, 567, 648, 810, 948
HpySE526I ACGT 2 cut(s) 287, 384
Hsp92II CATG 4 cut(s) 221, 514, 604, 746
Kzo9I GATC 4 cut(s) 634, 875, 1018, 1027
LguI GCTCTTC 1 cut(s) 58
LmnI GCTCC 2 cut(s) 811, 1063
Lsp1109I GCAGC 1 cut(s) 17
LweI GCATC 1 cut(s) 573
MaeI CTAG 1 cut(s) 339
MaeII ACGT 2 cut(s) 287, 384
MaeIII GTNAC 1 cut(s) 839
MalI GATC 4 cut(s) 636, 877, 1020, 1029
MboI GATC 4 cut(s) 634, 875, 1018, 1027
MfeI CAATTG 1 cut(s) 1008
MflI RGATCY 3 cut(s) 875, 1018, 1027
MhlI GDGCHC 1 cut(s) 954
MluCI AATT 7 cut(s) 202, 608, 797, 860, 883, 907, 1008
MlyI GAGTC 3 cut(s) 7, 272, 1052
Mph1103I ATGCAT 1 cut(s) 748
MroXI GAANNNNTTC 1 cut(s) 911
MseI TTAA 2 cut(s) 408, 695
MslI CAYNNNNRTG 2 cut(s) 435, 513
MspA1I CMGCKG 1 cut(s) 8
MunI CAATTG 1 cut(s) 1008
MwoI GCNNNNNNNGC 1 cut(s) 592
NdeI CATATG 1 cut(s) 757
NdeII GATC 4 cut(s) 634, 875, 1018, 1027
NlaIII CATG 4 cut(s) 221, 514, 604, 746
NlaIV GGNNCC 3 cut(s) 480, 989, 1029
NmuCI GTSAC 1 cut(s) 839
NsiI ATGCAT 1 cut(s) 748
NspI RCATGY 2 cut(s) 221, 514
OliI CACNNNNGTG 1 cut(s) 435
PaeI GCATGC 1 cut(s) 221
PceI AGGCCT 2 cut(s) 320, 971
PciI ACATGT 1 cut(s) 510
PciSI GCTCTTC 1 cut(s) 58
PdmI GAANNNNTTC 1 cut(s) 911
PfeI GAWTC 2 cut(s) 154, 787
PflFI GACNNNGTC 1 cut(s) 16
PkrI GCNGC 2 cut(s) 7, 10
PleI GAGTC 3 cut(s) 7, 271, 1051
PpsI GAGTC 3 cut(s) 7, 271, 1051
PpuMI RGGWCCY 1 cut(s) 105
PscI ACATGT 1 cut(s) 510
Psp1406I AACGTT 1 cut(s) 384
Psp5II RGGWCCY 1 cut(s) 105
PspN4I GGNNCC 3 cut(s) 480, 989, 1029
PspPI GGNCC 2 cut(s) 105, 739
PspPPI RGGWCCY 1 cut(s) 105
PsuI RGATCY 3 cut(s) 875, 1018, 1027
PsyI GACNNNGTC 1 cut(s) 16
RsaI GTAC 3 cut(s) 414, 459, 848
RsaNI GTAC 3 cut(s) 413, 458, 847
RseI CAYNNNNRTG 2 cut(s) 435, 513
SapI GCTCTTC 1 cut(s) 58
SaqAI TTAA 2 cut(s) 408, 695
SatI GCNGC 2 cut(s) 6, 9
Sau3AI GATC 4 cut(s) 634, 875, 1018, 1027
Sau96I GGNCC 2 cut(s) 105, 739
SchI GAGTC 3 cut(s) 7, 272, 1052
SduI GDGCHC 1 cut(s) 954
SfaNI GCATC 1 cut(s) 573
SinI GGWCC 1 cut(s) 105
SmiMI CAYNNNNRTG 2 cut(s) 435, 513
SphI GCATGC 1 cut(s) 221
Sse9I AATT 7 cut(s) 202, 608, 797, 860, 883, 907, 1008
SseBI AGGCCT 2 cut(s) 320, 971
SsiI CCGC 2 cut(s) 8, 659
SspMI CTAG 1 cut(s) 339
StuI AGGCCT 2 cut(s) 320, 971
StyI CCWWGG 2 cut(s) 1023, 1032
TaaI ACNGT 5 cut(s) 28, 403, 437, 682, 956
TaiI ACGT 2 cut(s) 290, 387
TaqI TCGA 3 cut(s) 23, 889, 915
TasI AATT 7 cut(s) 202, 608, 797, 860, 883, 907, 1008
TatI WGTACW 2 cut(s) 457, 846
TauI GCSGC 1 cut(s) 11
TfiI GAWTC 2 cut(s) 154, 787
Tru1I TTAA 2 cut(s) 408, 695
Tru9I TTAA 2 cut(s) 408, 695
TscAI CASTG 4 cut(s) 316, 408, 838, 959
TseFI GTSAC 1 cut(s) 839
TseI GCWGC 1 cut(s) 5
Tsp45I GTSAC 1 cut(s) 839
TspDTI ATGAA 5 cut(s) 252, 309, 873, 957, 980
TspGWI ACGGA 2 cut(s) 410, 1093
TspRI CASTG 4 cut(s) 316, 408, 838, 959
Tth111I GACNNNGTC 1 cut(s) 16
VpaK11BI GGWCC 1 cut(s) 105
XapI RAATTY 3 cut(s) 202, 860, 907
XceI RCATGY 2 cut(s) 221, 514
XmnI GAANNNNTTC 1 cut(s) 911
XspI CTAG 1 cut(s) 339
Zsp2I ATGCAT 1 cut(s) 748
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.