AT4G22410

ubiquitin carboxyl-terminal hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0002617
Physical Location & Seq
Reverse (-)
11818033 .. 11826143
8111 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G22410.2

Sequence Viewer

Length: 1110 bp
ATGGTTGAATTTCAGGTGTTGGATTTTCATTTTGAGAGGTTTTGCTCTGTGTCCTTGTCCAATCTGAATGTATATGCATGCCTTGTTTGTGGAAAGTACTTCCAAGGAAGAAGCCAGAAGTCTCATGCTTATACGCATAGCTTAGAAGCAGGACACCACGTTTACATCAATCTTCTGACAGAGAAGGTTTATTGTCTTCCTGATAGTTACGAGATCAATGACCCTTCTTTGGATGACATTCGCCATGTTTTAAACCCAAGGTTTAGTAGAGCACAAGTAAACGAGCTTGACAAGAATAGGCAGTGGTCTAGGGCTCTTGATGGCTCCGACTATCTTCCAGGAATGGTGGGGTTGAACAACATACAAAAGACAGAGTTTGTGAATGTTACAATCCAGTCGTTGATGAGAGTTACTCCTTTAAGGAATTTCTTCCACATTCCTGAAAATTATCAGCACTGCAAGTCTCCTCTTGTTCACTGTTTTGGGGAACTAACTCGCAAGATTTGGCATGCTCGAAACTTTAAAGGACAGGTGAGTCCACATGAGTTCTTGCAAGCTGTCATGAAAGCCAGCAAGAAACGCTTTCGGATTGGCCAACAATCAGATCCAGTAGAGTTTATGTCGTGGCTCCTCAACACTTTGCACATGGATCTTAGAACTTCAAAGGACGCCAGCAGTATCATCCACAAGTGCTTCCAGGGTGAGTTGGAGGTTGTGAAAGAGTTTCAAGGCAATGAAAACAAAGAAATCTCCAGGATGTCTTTTCTGATGCTCGGTCTAGATTTGCCACCGCCTCCTCTTTTCAAAGATGTCATGGAGAAAAACATTATTCCGCAGGTTGCTCTATTTGATTTATTGAAGAAGTTTGATGGAGAAACTGTGACAGAGGTGGTTCGGCCTAAGCTGGCCAGAATGAGATACCGTGTAATCAAATCTCCTCGTTACTTGATGTTCCATATGGTTCGGTTCAAGAAGAATAACTTCTTCAAAGAGAAGAACCCTACATTGGTTAACTTCCCAGTGAAGGACATGGAGCTTAGGGATTACATACCATCATTGCCTAGAGCACCAGAAGGGGAGAAGGTGTGTTCTTCTTTGTTTGTCTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
UCH zf-UBP

Protein Analysis

369

Amino Acids

43.15

Weight (kDa)

9.11

Isoelectric Point (pI)

37.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UCH PF00443 2 - 235 3.7e-28 Ubiquitin carboxyl-terminal hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 534
Acc36I ACCTGC 1 cut(s) 826
AciI CCGC 2 cut(s) 791, 833
AclWI GGATC 2 cut(s) 599, 657
AcoI YGGCCR 2 cut(s) 592, 906
AcsI RAATTY 2 cut(s) 8, 424
AcyI GRCGYC 1 cut(s) 669
AfaI GTAC 1 cut(s) 98
AfiI CCNNNNNNNGG 3 cut(s) 229, 1006, 1024
AgsI TTSAA 8 cut(s) 8, 355, 663, 728, 805, 859, 970, 988
AjnI CCWGG 3 cut(s) 337, 696, 752
AjuI GAANNNNNNNTTGG 2 cut(s) 989, 1021
AluBI AGCT 5 cut(s) 141, 286, 557, 904, 1036
AluI AGCT 5 cut(s) 141, 286, 557, 904, 1036
Alw21I GWGCWC 2 cut(s) 274, 1069
Alw26I GTCTC 2 cut(s) 126, 468
AlwI GGATC 2 cut(s) 599, 657
AoxI GGCC 3 cut(s) 592, 896, 906
ApoI RAATTY 2 cut(s) 8, 424
Asp700I GAANNNNTTC 2 cut(s) 428, 980
AsuHPI GGTGA 2 cut(s) 544, 713
BalI TGGCCA 2 cut(s) 594, 908
BanII GRGCYC 1 cut(s) 316
BbsI GAAGAC 1 cut(s) 188
Bbv12I GWGCWC 2 cut(s) 274, 1069
BccI CCATC 3 cut(s) 314, 863, 1060
BciT130I CCWGG 3 cut(s) 339, 698, 754
BcoDI GTCTC 2 cut(s) 126, 468
BfaI CTAG 3 cut(s) 309, 779, 1062
BfuAI ACCTGC 1 cut(s) 826
BmcAI AGTACT 1 cut(s) 98
Bme1390I CCNGG 3 cut(s) 339, 698, 754
BmiI GGNNCC 2 cut(s) 325, 629
BmrFI CCNGG 3 cut(s) 339, 698, 754
BmrI ACTGGG 1 cut(s) 1013
BmsI GCATC 1 cut(s) 759
BmuI ACTGGG 1 cut(s) 1013
BpiI GAAGAC 1 cut(s) 188
BplI GAGNNNNNCTC 2 cut(s) 397, 429
BpmI CTGGAG 1 cut(s) 736
Bpu10I CCTNAGC 2 cut(s) 900, 1037
BsaHI GRCGYC 1 cut(s) 669
BsaJI CCNNGG 3 cut(s) 103, 257, 697
BsaXI ACNNNNNCTCC 4 cut(s) 864, 894, 1070, 1100
Bsc4I CCNNNNNNNGG 3 cut(s) 229, 1006, 1024
Bse1I ACTGG 3 cut(s) 394, 608, 1019
Bse3DI GCAATG 2 cut(s) 739, 1055
BseBI CCWGG 3 cut(s) 339, 698, 754
BseDI CCNNGG 3 cut(s) 103, 257, 697
BseGI GGATG 3 cut(s) 238, 681, 762
BseLI CCNNNNNNNGG 3 cut(s) 229, 1006, 1024
BseMI GCAATG 2 cut(s) 739, 1055
BseNI ACTGG 3 cut(s) 394, 608, 1019
BseRI GAGGAG 4 cut(s) 456, 620, 786, 927
BshFI GGCC 3 cut(s) 594, 898, 908
BsiHKAI GWGCWC 2 cut(s) 274, 1069
BslI CCNNNNNNNGG 3 cut(s) 229, 1006, 1024
BsmAI GTCTC 2 cut(s) 126, 468
BsnI GGCC 3 cut(s) 594, 898, 908
Bsp1286I GDGCHC 3 cut(s) 274, 316, 1069
Bsp143I GATC 3 cut(s) 213, 604, 649
BspACI CCGC 2 cut(s) 791, 833
BspANI GGCC 3 cut(s) 594, 898, 908
BspHI TCATGA 1 cut(s) 561
BspLI GGNNCC 2 cut(s) 325, 629
BspMI ACCTGC 1 cut(s) 826
BspPI GGATC 2 cut(s) 599, 657
BsrDI GCAATG 2 cut(s) 739, 1055
BsrI ACTGG 3 cut(s) 394, 608, 1019
BssECI CCNNGG 3 cut(s) 103, 257, 697
BssMI GATC 3 cut(s) 213, 604, 649
BssNI GRCGYC 1 cut(s) 669
BssT1I CCWWGG 2 cut(s) 103, 257
Bst2UI CCWGG 3 cut(s) 339, 698, 754
Bst4CI ACNGT 3 cut(s) 479, 880, 923
BstACI GRCGYC 1 cut(s) 669
BstC8I GCNNGC 6 cut(s) 79, 510, 555, 571, 673, 906
BstDEI CTNAG 4 cut(s) 142, 653, 900, 1037
BstF5I GGATG 3 cut(s) 238, 681, 762
BstKTI GATC 3 cut(s) 216, 607, 652
BstMAI GTCTC 2 cut(s) 126, 468
BstMBI GATC 3 cut(s) 213, 604, 649
BstMWI GCNNNNNNNGC 1 cut(s) 579
BstNI CCWGG 3 cut(s) 339, 698, 754
BstNSI RCATGY 2 cut(s) 81, 512
BstSCI CCNGG 3 cut(s) 337, 696, 752
BstV2I GAAGAC 1 cut(s) 188
BstX2I RGATCY 2 cut(s) 604, 649
BstYI RGATCY 2 cut(s) 604, 649
BsuRI GGCC 3 cut(s) 594, 898, 908
BtsCI GGATG 3 cut(s) 238, 681, 762
BtsI GCAGTG 2 cut(s) 308, 454
BtsIMutI CAGTG 4 cut(s) 308, 454, 475, 1026
BveI ACCTGC 1 cut(s) 826
Cac8I GCNNGC 6 cut(s) 79, 510, 555, 571, 673, 906
CciI TCATGA 1 cut(s) 561
CseI GACGC 1 cut(s) 677
Csp6I GTAC 1 cut(s) 97
CviAII CATG 9 cut(s) 78, 125, 245, 509, 542, 562, 646, 814, 1030
CviQI GTAC 1 cut(s) 97
DdeI CTNAG 4 cut(s) 142, 653, 900, 1037
DpnI GATC 3 cut(s) 215, 606, 651
DpnII GATC 3 cut(s) 213, 604, 649
DraI TTTAAA 2 cut(s) 252, 523
DrdI GACNNNNNNGTC 1 cut(s) 534
DseDI GACNNNNNNGTC 1 cut(s) 534
EaeI YGGCCR 2 cut(s) 592, 906
Eco130I CCWWGG 2 cut(s) 103, 257
Eco24I GRGCYC 1 cut(s) 316
EcoRII CCWGG 3 cut(s) 337, 696, 752
EcoT14I CCWWGG 2 cut(s) 103, 257
EcoT22I ATGCAT 1 cut(s) 79
EcoT38I GRGCYC 1 cut(s) 316
ErhI CCWWGG 2 cut(s) 103, 257
FaeI CATG 9 cut(s) 81, 128, 248, 512, 545, 565, 649, 817, 1033
FalI AAGNNNNNCTT 4 cut(s) 566, 598, 965, 997
FatI CATG 9 cut(s) 77, 124, 244, 508, 541, 561, 645, 813, 1029
FauNDI CATATG 1 cut(s) 957
FokI GGATG 3 cut(s) 245, 668, 769
FriOI GRGCYC 1 cut(s) 316
FspBI CTAG 3 cut(s) 309, 779, 1062
GsuI CTGGAG 1 cut(s) 736
HaeIII GGCC 3 cut(s) 594, 898, 908
HgaI GACGC 1 cut(s) 677
Hin1I GRCGYC 1 cut(s) 669
Hin1II CATG 9 cut(s) 81, 128, 248, 512, 545, 565, 649, 817, 1033
HincII GTYRAC 1 cut(s) 1012
HindII GTYRAC 1 cut(s) 1012
HinfI GANTC 1 cut(s) 535
HpaI GTTAAC 1 cut(s) 1012
HphI GGTGA 2 cut(s) 544, 713
Hpy166II GTNNAC 5 cut(s) 163, 280, 475, 539, 1012
Hpy188I TCNGA 6 cut(s) 66, 177, 328, 588, 604, 768
Hpy188III TCNNGA 6 cut(s) 200, 317, 440, 562, 779, 970
Hpy8I GTNNAC 5 cut(s) 163, 280, 475, 539, 1012
HpyAV CCTTC 5 cut(s) 178, 234, 1018, 1067, 1075
HpyCH4III ACNGT 3 cut(s) 479, 880, 923
HpyCH4IV ACGT 1 cut(s) 159
HpyCH4V TGCA 4 cut(s) 77, 459, 553, 643
HpyF10VI GCNNNNNNNGC 1 cut(s) 579
HpyF3I CTNAG 4 cut(s) 142, 653, 900, 1037
HpySE526I ACGT 1 cut(s) 159
Hsp92I GRCGYC 1 cut(s) 669
Hsp92II CATG 9 cut(s) 81, 128, 248, 512, 545, 565, 649, 817, 1033
KspAI GTTAAC 1 cut(s) 1012
Kzo9I GATC 3 cut(s) 213, 604, 649
LmnI GCTCC 3 cut(s) 329, 633, 1033
LweI GCATC 1 cut(s) 759
MaeI CTAG 3 cut(s) 309, 779, 1062
MaeII ACGT 1 cut(s) 159
MaeIII GTNAC 5 cut(s) 206, 385, 409, 880, 941
MalI GATC 3 cut(s) 215, 606, 651
MboI GATC 3 cut(s) 213, 604, 649
MflI RGATCY 2 cut(s) 604, 649
MhlI GDGCHC 3 cut(s) 274, 316, 1069
MlsI TGGCCA 2 cut(s) 594, 908
MluCI AATT 3 cut(s) 8, 424, 445
MluNI TGGCCA 2 cut(s) 594, 908
MlyI GAGTC 1 cut(s) 544
MmeI TCCRAC 2 cut(s) 351, 687
MnlI CCTC 8 cut(s) 30, 477, 641, 703, 804, 807, 880, 948
Mox20I TGGCCA 2 cut(s) 594, 908
Mph1103I ATGCAT 1 cut(s) 79
MroXI GAANNNNTTC 2 cut(s) 428, 980
MscI TGGCCA 2 cut(s) 594, 908
MseI TTAA 4 cut(s) 251, 419, 522, 1011
Msp20I TGGCCA 2 cut(s) 594, 908
MspR9I CCNGG 3 cut(s) 339, 698, 754
MvaI CCWGG 3 cut(s) 339, 698, 754
MwoI GCNNNNNNNGC 1 cut(s) 579
NdeI CATATG 1 cut(s) 957
NdeII GATC 3 cut(s) 213, 604, 649
NlaIII CATG 9 cut(s) 81, 128, 248, 512, 545, 565, 649, 817, 1033
NlaIV GGNNCC 2 cut(s) 325, 629
NmuCI GTSAC 1 cut(s) 880
NsiI ATGCAT 1 cut(s) 79
NspI RCATGY 2 cut(s) 81, 512
PaeI GCATGC 2 cut(s) 81, 512
PagI TCATGA 1 cut(s) 561
PdmI GAANNNNTTC 2 cut(s) 428, 980
PfoI TCCNGGA 2 cut(s) 337, 752
PleI GAGTC 1 cut(s) 543
PpsI GAGTC 1 cut(s) 543
Psp6I CCWGG 3 cut(s) 337, 696, 752
PspGI CCWGG 3 cut(s) 337, 696, 752
PspN4I GGNNCC 2 cut(s) 325, 629
PsuI RGATCY 2 cut(s) 604, 649
RsaI GTAC 1 cut(s) 98
RsaNI GTAC 1 cut(s) 97
SaqAI TTAA 4 cut(s) 251, 419, 522, 1011
Sau3AI GATC 3 cut(s) 213, 604, 649
ScaI AGTACT 1 cut(s) 98
SchI GAGTC 1 cut(s) 544
ScrFI CCNGG 3 cut(s) 339, 698, 754
SduI GDGCHC 3 cut(s) 274, 316, 1069
SfaNI GCATC 1 cut(s) 759
SphI GCATGC 2 cut(s) 81, 512
Sse9I AATT 3 cut(s) 8, 424, 445
SsiI CCGC 2 cut(s) 791, 833
SspMI CTAG 3 cut(s) 309, 779, 1062
StyD4I CCNGG 3 cut(s) 337, 696, 752
StyI CCWWGG 2 cut(s) 103, 257
TaaI ACNGT 3 cut(s) 479, 880, 923
TaiI ACGT 1 cut(s) 162
TaqI TCGA 1 cut(s) 514
TaqII GACCGA 1 cut(s) 764
TasI AATT 3 cut(s) 8, 424, 445
TatI WGTACW 1 cut(s) 96
Tru1I TTAA 4 cut(s) 251, 419, 522, 1011
Tru9I TTAA 4 cut(s) 251, 419, 522, 1011
TscAI CASTG 4 cut(s) 308, 461, 482, 1026
TseFI GTSAC 1 cut(s) 880
Tsp45I GTSAC 1 cut(s) 880
TspDTI ATGAA 3 cut(s) 17, 578, 750
TspRI CASTG 4 cut(s) 308, 461, 482, 1026
XapI RAATTY 2 cut(s) 8, 424
XbaI TCTAGA 1 cut(s) 778
XceI RCATGY 2 cut(s) 81, 512
XmnI GAANNNNTTC 2 cut(s) 428, 980
XspI CTAG 3 cut(s) 309, 779, 1062
ZrmI AGTACT 1 cut(s) 98
Zsp2I ATGCAT 1 cut(s) 79
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.