Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0002617

Physical Location & Seq

Genomic Coordinates Reverse (-)

40942168 .. 40946504

Gene Structure 4337 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1331700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1680 bp

ATGAGCTCTAAGAGGGAACATGAAGGTAATGCTAGTGTGAACACGGAAGGGTTTGAATTGCATCCGAAGAGGCAAAAGCTGAATGAGCATTCATCGCCGTCACCTGCCTCTGAAGGCACTCTACTTCCGGGTTTTAATTACGGAGATGTGGACGACGAGGACAACCACAGAAGGCCACCTGTGAGTGGCTTTAACGGGGACGGCGGCGATAATCAAAAGGGTGTACAAAATGGGGGTCAAGTTGAAGAACTAGAGGATGATGATGATGATGACCTTGAACAAGGACTTTACCAAAGGCGGGGACGTGAAATCGAGGTCAGGAGGGATTGCCCCTATCTTGACACTGTCAATCGCCAGGTTTTGGATTTCGATTTTGAGAAGTTCTGCTCTCTGTCACTGTCAAATTTGAATGTGTATGCATGCTTGGTTTGTGGGAAGTATTACCAAGGCAGAGGGAAGAAGTCCCATGCATATACTCATAGCCTTGAAGCTGGGCACCATGTTTATATCAATCTCCGAACAGAGAAAGTTTATTGCCTTCCCGATGGATATGAAATAAGTGACCCATCGTTGGATGATATTCGACACGTCCTAAACCCAAGGTTTACTGATGAACAAGTTGAGCAGATTGACAAGAACAAGCAATGGTCTAGAGCACTTGATGGTTCTGATTACCTTCCTGGAATGGTGGGGCTCAATAACATCAAGGAGACTGATTTTGTGAATGTCACGATTCAATCTTTAATGAGAGTCACTCCTCTAAGAAACTTTTTCCTTATCCCTAAGAATTATCAACATTGCAAGTCTCCGCTTGTTCATCGATTTGGGGAGCTAACGAGAAAGATATGGCATGCACGAAACTTCAAAGGACAGGTCAGCCCACATGAGTTCCTACAGGCAGTTATGAAAGCCAGTAAGAAAAGGTTTCGAATTGGAGCACAGTCTGACCCAGTCGAATTCATATCATGGCTGCTTAATACGCTGCATGCGGCTCTTAGAACTTCAAAGAAAAATAGCAGCATCATATATGAGTGCTTTCAGGGGGAACTGGAGGTTATGAAGGAGATTCCTAATAAAGGTATTGAGAAGATAGAAAATGGTGATGACCAGAACTCAGGTGCTGCAACTGAAAATAAGGGCATTGTCAAGGAAACTTGCAAAATGCCTTTCTTGATGCTTGGATTGGATTTGCCCCCACCACCGCTTTTCAAAGATGTGATGGAGAAAAATATAATACCCCAGGTCCCACTCTTCAACATTTTGAAGAAATTTGATGGGGAGACAGTCACTGAAGTGGTCCGACCTCGCATATCAAGGATGAGGTATCGAGTCACCCGATTACCTCAGTATCTTATTCTCCACATGCAACGTTTTACAAAGAACAATTTCTTTGTGGAGAAGAATCCCACACTAGTGAACTTTCCTGTGAAGAACCTCGAATTGAAAGATTACATCCCGTTGTCAACCACCAAAGAGAACGAGAGGCTGCGCTCAAAGTATGATCTCATTGCCAACATTGTCCATGACGGAAAACCTGGTGAAGGTTCCTACAGGGTGTTTGTACAAAGAAAATCTGAAGAGCTATGGTATGAGATGCAGGACCTTCATGTCTCCGAAACCCTTCCTCAAATGGTTGCGCTGTCAGAGACATATATGCAGATATACGAGCAGCAGCAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0031974 GO:0031981 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0070013

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

UCH zf-UBP

Protein Analysis

560

Amino Acids

64.48

Weight (kDa)

6.22

Isoelectric Point (pI)

44.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-UBP	PF02148	129 - 190	2.4e-19	Zn-finger in ubiquitin-hydrolases and other protein
UCH	PF00443	230 - 555	1.5e-43	Ubiquitin carboxyl-terminal hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002617)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G22285 AT4G22285 AT4G22350 AT4G22350 AT4G22350 AT4G22350 AT4G22410 AT4G22410
fragaria_vesca	FvH4_3g00980 FvH4_3g00980 FvH4_3g00980 FvH4_3g00980 FvH4_3g00980 FvH4_3g00980 FvH4_3g00980 FvH4_3g00980 FvH4_3g00980 FvH4_3g00980 FvH4_6g03150
malus_domestica	MD05G1357700.v1.1 MD10G1331700.v1.1
prunus_persica	Prupe.4G010400_v2.0.a1 Prupe.4G010400_v2.0.a1 Prupe.4G010400_v2.0.a1 Prupe.4G010400_v2.0.a1 Prupe.4G010400_v2.0.a1
pyrus_communis	pycom05g32360 pycom10g28130
rosa_chinensis	RchiOBHm_Chr4g0397451 RchiOBHm_Chr5g0001501 RchiOBHm_Chr5g0001511
rosa_laevigata	RLG00000030934
rosa_multiflora	Rmu_sc0003749.1_g000009
rosa_roxburghii	Rroxscaffold_1G00074880
rosa_rugosa	Rorug04G0390700
rosa_samantha	Rh5AG011700 Rh5BG014200 Rh5CG012700 Rh5DG012000
rosa_wichuraiana	Rw5G001110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	112
AasI	GACNNNNNNGTC	1 cut(s)	1607
Acc36I	ACCTGC	1 cut(s)	112
AccB1I	GGYRCC	1 cut(s)	495
AccB7I	CCANNNNNTGG	1 cut(s)	361
AciI	CCGC	5 cut(s)	204, 298, 809, 989, 1202
AclI	AACGTT	1 cut(s)	1369
AcsI	RAATTY	3 cut(s)	403, 956, 1268
AcuI	CTGAAG	3 cut(s)	132, 1311, 1596
AfaI	GTAC	2 cut(s)	225, 1563
AfiI	CCNNNNNNNGG	6 cut(s)	185, 298, 361, 571, 1076, 1541
AflIII	ACRYGT	1 cut(s)	586
AhlI	ACTAGT	1 cut(s)	1411
AjiI	CACGTC	2 cut(s)	305, 589
AjnI	CCWGG	4 cut(s)	354, 679, 1239, 1534
AleI	CACNNNNGTG	2 cut(s)	1292, 1412
AluBI	AGCT	5 cut(s)	6, 79, 491, 832, 1582
AluI	AGCT	5 cut(s)	6, 79, 491, 832, 1582
Alw21I	GWGCWC	3 cut(s)	8, 658, 940
Alw26I	GTCTC	5 cut(s)	704, 810, 1274, 1615, 1640
AlwNI	CAGNNNCTG	2 cut(s)	1121, 1289
AoxI	GGCC	1 cut(s)	173
ApeKI	GCWGC	7 cut(s)	970, 982, 1017, 1121, 1486, 1669, 1672
ApoI	RAATTY	3 cut(s)	403, 956, 1268
ArsI	GACNNNNNNTTYG	4 cut(s)	220, 252, 858, 890
Asp700I	GAANNNNTTC	2 cut(s)	1385, 1620
AspLEI	GCGC	2 cut(s)	1491, 1639
AspS9I	GGNCC	3 cut(s)	1243, 1297, 1600
AsuC2I	CCSGG	1 cut(s)	129
AsuHPI	GGTGA	4 cut(s)	93, 1112, 1324, 1550
AsuII	TTCGAA	1 cut(s)	928
AvaII	GGWCC	3 cut(s)	1243, 1297, 1600
BaeGI	GKGCMC	1 cut(s)	498
BaeI	ACNNNNGTAYC	2 cut(s)	1331, 1364
BanI	GGYRCC	1 cut(s)	495
BanII	GRGCYC	2 cut(s)	8, 696
BarI	GAAGNNNNNNTAC	2 cut(s)	105, 137
Bbv12I	GWGCWC	3 cut(s)	8, 658, 940
BbvI	GCAGC	5 cut(s)	957, 969, 1029, 1108, 1473
BccI	CCATC	5 cut(s)	539, 574, 656, 1213, 1268
BceAI	ACGGC	2 cut(s)	82, 217
BciT130I	CCWGG	4 cut(s)	356, 681, 1241, 1536
BcnI	CCSGG	1 cut(s)	129
BcoDI	GTCTC	5 cut(s)	704, 810, 1274, 1615, 1640
BcuI	ACTAGT	1 cut(s)	1411
BfaI	CTAG	4 cut(s)	33, 251, 651, 1412
BfmI	CTRYAG	2 cut(s)	893, 1549
BfuAI	ACCTGC	1 cut(s)	112
BisI	GCNGC	9 cut(s)	205, 971, 983, 990, 1018, 1122, 1487, 1670, 1673
BlsI	GCNGC	9 cut(s)	206, 972, 984, 991, 1019, 1123, 1488, 1671, 1674
Bme1390I	CCNGG	5 cut(s)	129, 356, 681, 1241, 1536
Bme18I	GGWCC	3 cut(s)	1243, 1297, 1600
BmgBI	CACGTC	2 cut(s)	305, 589
BmgT120I	GGNCC	3 cut(s)	1243, 1297, 1600
BmiI	GGNNCC	3 cut(s)	497, 1245, 1546
BmrFI	CCNGG	5 cut(s)	129, 356, 681, 1241, 1536
BmrI	ACTGGG	1 cut(s)	944
BmsI	GCATC	4 cut(s)	70, 1029, 1164, 1584
BmuI	ACTGGG	1 cut(s)	944
BplI	GAGNNNNNCTC	2 cut(s)	739, 771
BpmI	CTGGAG	1 cut(s)	1070
Bpu14I	TTCGAA	1 cut(s)	928
BpuMI	CCSGG	1 cut(s)	129
Bsa29I	ATCGAT	1 cut(s)	820
BsaJI	CCNNGG	3 cut(s)	445, 599, 1239
Bsc4I	CCNNNNNNNGG	6 cut(s)	185, 298, 361, 571, 1076, 1541
Bse1I	ACTGG	3 cut(s)	912, 950, 1053
Bse3DI	GCAATG	3 cut(s)	650, 796, 1506
BseBI	CCWGG	4 cut(s)	356, 681, 1241, 1536
BseCI	ATCGAT	1 cut(s)	820
BseDI	CCNNGG	3 cut(s)	445, 599, 1239
BseGI	GGATG	5 cut(s)	61, 262, 580, 1323, 1452
BseLI	CCNNNNNNNGG	6 cut(s)	185, 298, 361, 571, 1076, 1541
BseMI	GCAATG	3 cut(s)	650, 796, 1506
BseMII	CTCAG	2 cut(s)	1128, 1358
BseNI	ACTGG	3 cut(s)	912, 950, 1053
BseRI	GAGGAG	1 cut(s)	747
BseSI	GKGCMC	1 cut(s)	498
BseXI	GCAGC	5 cut(s)	957, 969, 1029, 1108, 1473
BseYI	CCCAGC	1 cut(s)	491
BshFI	GGCC	1 cut(s)	175
BshNI	GGYRCC	1 cut(s)	495
BshVI	ATCGAT	1 cut(s)	820
BsiHKAI	GWGCWC	3 cut(s)	8, 658, 940
BsiSI	CCGG	1 cut(s)	128
BslFI	GGGAC	4 cut(s)	212, 315, 448, 1229
BslI	CCNNNNNNNGG	6 cut(s)	185, 298, 361, 571, 1076, 1541
BsmAI	GTCTC	5 cut(s)	704, 810, 1274, 1615, 1640
BsmFI	GGGAC	4 cut(s)	212, 315, 448, 1229
BsmI	GAATGC	1 cut(s)	88
BsnI	GGCC	1 cut(s)	175
Bsp119I	TTCGAA	1 cut(s)	928
Bsp1286I	GDGCHC	5 cut(s)	8, 498, 658, 696, 940
Bsp1407I	TGTACA	2 cut(s)	223, 1561
Bsp143I	GATC	1 cut(s)	1501
BspACI	CCGC	5 cut(s)	204, 298, 809, 989, 1202
BspANI	GGCC	1 cut(s)	175
BspCNI	CTCAG	2 cut(s)	1127, 1357
BspDI	ATCGAT	1 cut(s)	820
BspLI	GGNNCC	3 cut(s)	497, 1245, 1546
BspMI	ACCTGC	1 cut(s)	112
BspQI	GCTCTTC	1 cut(s)	1572
BspT104I	TTCGAA	1 cut(s)	928
BspT107I	GGYRCC	1 cut(s)	495
BsrDI	GCAATG	3 cut(s)	650, 796, 1506
BsrGI	TGTACA	2 cut(s)	223, 1561
BsrI	ACTGG	3 cut(s)	912, 950, 1053
BssECI	CCNNGG	3 cut(s)	445, 599, 1239
BssMI	GATC	1 cut(s)	1501
BssT1I	CCWWGG	2 cut(s)	445, 599
Bst2UI	CCWGG	4 cut(s)	356, 681, 1241, 1536
Bst4CI	ACNGT	4 cut(s)	346, 399, 942, 1285
Bst6I	CTCTTC	3 cut(s)	62, 1256, 1572
BstAUI	TGTACA	2 cut(s)	223, 1561
BstBI	TTCGAA	1 cut(s)	928
BstC8I	GCNNGC	3 cut(s)	421, 852, 987
BstDEI	CTNAG	6 cut(s)	9, 761, 783, 995, 1114, 1344
BstENI	CCTNNNNNAGG	2 cut(s)	1074, 1539
BstF5I	GGATG	5 cut(s)	61, 262, 580, 1323, 1452
BstHHI	GCGC	2 cut(s)	1491, 1639
BstKTI	GATC	1 cut(s)	1504
BstMAI	GTCTC	5 cut(s)	704, 810, 1274, 1615, 1640
BstMBI	GATC	1 cut(s)	1501
BstMWI	GCNNNNNNNGC	3 cut(s)	85, 94, 979
BstNI	CCWGG	4 cut(s)	356, 681, 1241, 1536
BstNSI	RCATGY	4 cut(s)	423, 854, 989, 1366
BstSCI	CCNGG	5 cut(s)	127, 354, 679, 1239, 1534
BstSFI	CTRYAG	2 cut(s)	893, 1549
BstSLI	GKGCMC	1 cut(s)	498
BstV1I	GCAGC	5 cut(s)	957, 969, 1029, 1108, 1473
Bsu15I	ATCGAT	1 cut(s)	820
BsuRI	GGCC	1 cut(s)	175
BsuTUI	ATCGAT	1 cut(s)	820
BtgZI	GCGATG	1 cut(s)	78
BtrI	CACGTC	2 cut(s)	305, 589
BtsCI	GGATG	5 cut(s)	61, 262, 580, 1323, 1452
BtsIMutI	CAGTG	3 cut(s)	342, 395, 1287
BveI	ACCTGC	1 cut(s)	112
Cac8I	GCNNGC	3 cut(s)	421, 852, 987
CaiI	CAGNNNCTG	2 cut(s)	1121, 1289
CfoI	GCGC	2 cut(s)	1491, 1639
Cfr13I	GGNCC	3 cut(s)	1243, 1297, 1600
ClaI	ATCGAT	1 cut(s)	820
CsiI	ACCWGGT	1 cut(s)	1534
Csp6I	GTAC	2 cut(s)	224, 1562
CviQI	GTAC	2 cut(s)	224, 1562
DdeI	CTNAG	6 cut(s)	9, 761, 783, 995, 1114, 1344
DpnI	GATC	1 cut(s)	1503
DpnII	GATC	1 cut(s)	1501
DrdI	GACNNNNNNGTC	1 cut(s)	1607
DseDI	GACNNNNNNGTC	1 cut(s)	1607
Eam1104I	CTCTTC	3 cut(s)	62, 1256, 1572
EarI	CTCTTC	3 cut(s)	62, 1256, 1572
Ecl136II	GAGCTC	1 cut(s)	6
Eco130I	CCWWGG	2 cut(s)	445, 599
Eco24I	GRGCYC	2 cut(s)	8, 696
Eco47I	GGWCC	3 cut(s)	1243, 1297, 1600
Eco53kI	GAGCTC	1 cut(s)	6
Eco57I	CTGAAG	3 cut(s)	132, 1311, 1596
EcoICRI	GAGCTC	1 cut(s)	6
EcoNI	CCTNNNNNAGG	2 cut(s)	1074, 1539
EcoO109I	RGGNCCY	2 cut(s)	1243, 1600
EcoRI	GAATTC	1 cut(s)	956
EcoRII	CCWGG	4 cut(s)	354, 679, 1239, 1534
EcoT14I	CCWWGG	2 cut(s)	445, 599
EcoT22I	ATGCAT	2 cut(s)	421, 472
EcoT38I	GRGCYC	2 cut(s)	8, 696
ErhI	CCWWGG	2 cut(s)	445, 599
FaqI	GGGAC	4 cut(s)	212, 315, 448, 1229
FauI	CCCGC	1 cut(s)	291
Fnu4HI	GCNGC	9 cut(s)	205, 971, 983, 990, 1018, 1122, 1487, 1670, 1673
FokI	GGATG	5 cut(s)	48, 269, 587, 1330, 1439
FriOI	GRGCYC	2 cut(s)	8, 696
Fsp4HI	GCNGC	9 cut(s)	205, 971, 983, 990, 1018, 1122, 1487, 1670, 1673
FspBI	CTAG	4 cut(s)	33, 251, 651, 1412
GlaI	GCGC	2 cut(s)	1490, 1638
GluI	GCNGC	9 cut(s)	205, 971, 983, 990, 1018, 1122, 1487, 1670, 1673
GsaI	CCCAGC	1 cut(s)	495
GsuI	CTGGAG	1 cut(s)	1070
HaeIII	GGCC	1 cut(s)	175
HapII	CCGG	1 cut(s)	128
HhaI	GCGC	2 cut(s)	1491, 1639
Hin6I	GCGC	2 cut(s)	1489, 1637
HinP1I	GCGC	2 cut(s)	1489, 1637
HincII	GTYRAC	1 cut(s)	1464
HindII	GTYRAC	1 cut(s)	1464
HinfI	GANTC	5 cut(s)	733, 750, 1066, 1329, 1402
HpaII	CCGG	1 cut(s)	128
HphI	GGTGA	4 cut(s)	93, 1112, 1324, 1550
Hpy166II	GTNNAC	6 cut(s)	40, 151, 224, 606, 1417, 1464
Hpy188I	TCNGA	9 cut(s)	66, 112, 518, 670, 946, 1301, 1576, 1615, 1645
Hpy188III	TCNNGA	6 cut(s)	319, 338, 542, 651, 730, 1171
Hpy8I	GTNNAC	6 cut(s)	40, 151, 224, 606, 1417, 1464
Hpy99I	CGWCG	1 cut(s)	158
HpyCH4III	ACNGT	4 cut(s)	346, 399, 942, 1285
HpyCH4IV	ACGT	3 cut(s)	304, 588, 1369
HpyF10VI	GCNNNNNNNGC	3 cut(s)	85, 94, 979
HpyF3I	CTNAG	6 cut(s)	9, 761, 783, 995, 1114, 1344
HpySE526I	ACGT	3 cut(s)	304, 588, 1369
HspAI	GCGC	2 cut(s)	1489, 1637
Kzo9I	GATC	1 cut(s)	1501
LguI	GCTCTTC	1 cut(s)	1572
LmnI	GCTCC	2 cut(s)	829, 935
Lsp1109I	GCAGC	5 cut(s)	957, 969, 1029, 1108, 1473
LweI	GCATC	4 cut(s)	70, 1029, 1164, 1584
MabI	ACCWGGT	1 cut(s)	1534
MaeI	CTAG	4 cut(s)	33, 251, 651, 1412
MaeII	ACGT	3 cut(s)	304, 588, 1369
MaeIII	GTNAC	7 cut(s)	99, 393, 560, 727, 751, 1285, 1330
MalI	GATC	1 cut(s)	1503
MboI	GATC	1 cut(s)	1501
MboII	GAAGA	9 cut(s)	79, 257, 469, 1099, 1243, 1276, 1411, 1441, 1589
MhlI	GDGCHC	5 cut(s)	8, 498, 658, 696, 940
MluCI	AATT	9 cut(s)	56, 136, 403, 787, 930, 956, 1268, 1384, 1439
MlyI	GAGTC	2 cut(s)	759, 1338
MmeI	TCCRAC	2 cut(s)	552, 1324
Mph1103I	ATGCAT	2 cut(s)	421, 472
MroXI	GAANNNNTTC	2 cut(s)	1385, 1620
MseI	TTAA	4 cut(s)	135, 192, 743, 975
MslI	CAYNNNNRTG	2 cut(s)	1292, 1412
MspI	CCGG	1 cut(s)	128
MspR9I	CCNGG	5 cut(s)	129, 356, 681, 1241, 1536
Mva1269I	GAATGC	1 cut(s)	88
MvaI	CCWGG	4 cut(s)	356, 681, 1241, 1536
MwoI	GCNNNNNNNGC	3 cut(s)	85, 94, 979
NciI	CCSGG	1 cut(s)	129
NdeII	GATC	1 cut(s)	1501
NlaIV	GGNNCC	3 cut(s)	497, 1245, 1546
NmuCI	GTSAC	7 cut(s)	99, 393, 560, 727, 751, 1285, 1330
NsiI	ATGCAT	2 cut(s)	421, 472
NspI	RCATGY	4 cut(s)	423, 854, 989, 1366
NspV	TTCGAA	1 cut(s)	928
OliI	CACNNNNGTG	2 cut(s)	1292, 1412
PaeI	GCATGC	3 cut(s)	423, 854, 989
PaqCI	CACCTGC	1 cut(s)	112
PciSI	GCTCTTC	1 cut(s)	1572
PcsI	WCGNNNNNNNCGW	1 cut(s)	1333
PctI	GAATGC	1 cut(s)	88
PdmI	GAANNNNTTC	2 cut(s)	1385, 1620
PfeI	GAWTC	3 cut(s)	733, 1066, 1402
PflFI	GACNNNGTC	2 cut(s)	344, 950
PflMI	CCANNNNNTGG	1 cut(s)	361
PfoI	TCCNGGA	1 cut(s)	679
PkrI	GCNGC	9 cut(s)	206, 972, 984, 991, 1019, 1123, 1488, 1671, 1674
PleI	GAGTC	2 cut(s)	758, 1337
PpsI	GAGTC	2 cut(s)	758, 1337
PpuMI	RGGWCCY	2 cut(s)	1243, 1600
Psp124BI	GAGCTC	1 cut(s)	8
Psp1406I	AACGTT	1 cut(s)	1369
Psp5II	RGGWCCY	2 cut(s)	1243, 1600
Psp6I	CCWGG	4 cut(s)	354, 679, 1239, 1534
PspFI	CCCAGC	1 cut(s)	491
PspGI	CCWGG	4 cut(s)	354, 679, 1239, 1534
PspN4I	GGNNCC	3 cut(s)	497, 1245, 1546
PspPI	GGNCC	3 cut(s)	1243, 1297, 1600
PspPPI	RGGWCCY	2 cut(s)	1243, 1600
PstNI	CAGNNNCTG	2 cut(s)	1121, 1289
PsyI	GACNNNGTC	2 cut(s)	344, 950
RsaI	GTAC	2 cut(s)	225, 1563
RsaNI	GTAC	2 cut(s)	224, 1562
RseI	CAYNNNNRTG	2 cut(s)	1292, 1412
SacI	GAGCTC	1 cut(s)	8
SapI	GCTCTTC	1 cut(s)	1572
SaqAI	TTAA	4 cut(s)	135, 192, 743, 975
SatI	GCNGC	9 cut(s)	205, 971, 983, 990, 1018, 1122, 1487, 1670, 1673
Sau3AI	GATC	1 cut(s)	1501
Sau96I	GGNCC	3 cut(s)	1243, 1297, 1600
SchI	GAGTC	2 cut(s)	759, 1338
ScrFI	CCNGG	5 cut(s)	129, 356, 681, 1241, 1536
SduI	GDGCHC	5 cut(s)	8, 498, 658, 696, 940
SexAI	ACCWGGT	1 cut(s)	1534
SfaNI	GCATC	4 cut(s)	70, 1029, 1164, 1584
SfcI	CTRYAG	2 cut(s)	893, 1549
SfuI	TTCGAA	1 cut(s)	928
SinI	GGWCC	3 cut(s)	1243, 1297, 1600
SmiMI	CAYNNNNRTG	2 cut(s)	1292, 1412
SpeI	ACTAGT	1 cut(s)	1411
SphI	GCATGC	3 cut(s)	423, 854, 989
Sse9I	AATT	9 cut(s)	56, 136, 403, 787, 930, 956, 1268, 1384, 1439
SsiI	CCGC	5 cut(s)	204, 298, 809, 989, 1202
SspMI	CTAG	4 cut(s)	33, 251, 651, 1412
SstI	GAGCTC	1 cut(s)	8
StyD4I	CCNGG	5 cut(s)	127, 354, 679, 1239, 1534
StyI	CCWWGG	2 cut(s)	445, 599
TaaI	ACNGT	4 cut(s)	346, 399, 942, 1285
TaiI	ACGT	3 cut(s)	307, 591, 1372
TaqI	TCGA	8 cut(s)	312, 369, 583, 820, 928, 954, 1327, 1437
TasI	AATT	9 cut(s)	56, 136, 403, 787, 930, 956, 1268, 1384, 1439
TatI	WGTACW	2 cut(s)	223, 1561
TauI	GCSGC	2 cut(s)	207, 992
TfiI	GAWTC	3 cut(s)	733, 1066, 1402
Tru1I	TTAA	4 cut(s)	135, 192, 743, 975
Tru9I	TTAA	4 cut(s)	135, 192, 743, 975
TscAI	CASTG	3 cut(s)	349, 402, 1294
TseFI	GTSAC	7 cut(s)	99, 393, 560, 727, 751, 1285, 1330
TseI	GCWGC	7 cut(s)	970, 982, 1017, 1121, 1486, 1669, 1672
Tsp45I	GTSAC	7 cut(s)	99, 393, 560, 727, 751, 1285, 1330
TspDTI	ATGAA	9 cut(s)	36, 81, 567, 627, 806, 920, 949, 1073, 1595
TspGWI	ACGGA	3 cut(s)	59, 156, 1542
TspRI	CASTG	3 cut(s)	349, 402, 1294
Tth111I	GACNNNGTC	2 cut(s)	344, 950
Van91I	CCANNNNNTGG	1 cut(s)	361
VpaK11BI	GGWCC	3 cut(s)	1243, 1297, 1600
XagI	CCTNNNNNAGG	2 cut(s)	1074, 1539
XapI	RAATTY	3 cut(s)	403, 956, 1268
XbaI	TCTAGA	1 cut(s)	650
XceI	RCATGY	4 cut(s)	423, 854, 989, 1366
XmnI	GAANNNNTTC	2 cut(s)	1385, 1620
XspI	CTAG	4 cut(s)	33, 251, 651, 1412
Zsp2I	ATGCAT	2 cut(s)	421, 472

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.