RchiOBHm_Chr5g0001501

U4 U6.U5 tri-snRNP-associated protein 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0002617
Physical Location & Seq
Forward (+)
787695 .. 788973
1279 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ28292

Sequence Viewer

Length: 714 bp
ATGAGTTCGAAGAGAGAGCATGAAGATAATGGTGGTGGGGGTATTGAAGGGGAAGGGAGTTTATTGGAGGCAAAGAGGCAGAAAGTGAGTGAGGAAGTATCGCCGGACTCTTCGCCGCCTGCCTCCCTTCTTCCTGGTTTTAATTATGGGGACGAGGATGAGGAGGAGAGAAGGGCACCTGTTAATGGAGGGGGTGATGGTAAGGAGCTACAGGCTGGAAAAAATGGGGTTCGAGTTGAAGAAGAAGAAGAAGAGGATGATGGTGAACAAGGACTTTATCAAGGACACAAGAGCCGCGAAATTGAAGTTAGGAGGGATTGCCCGTATCTTGATGCTGTTAATCGCCAGGTTTTGGATTTTGATTTTGAGAAGTTTTGCTCGCTTTCTCTATCAAATTTGAACGTGTATGCATGCTTGGTTTGTGGGAAGTATTACCAAGGAAGAGGAAGGAAGTCCCACGCATATACTCATAGCCTTGAAGCAGGACACCATGTGTATATCAATCTTCGAACAGAGAAGGTTTATTGCCTTCCTGATGGATATGAAATTAGTGACCCATCATTGGATGACATTCGACATGTTCTGAACCCAAGGTTTACTTATTTTGGTGTATTGTTTGTACTTCCACCTTCTCTTTCCCTTGCATCTTGTCTTTCTTTCACTTTATGTCTTATCTCAGGGAAGTGTGTGCAAACCTGCATCTTAAGTCGATAA

Protein Analysis

237

Amino Acids

26.35

Weight (kDa)

4.96

Isoelectric Point (pI)

64.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-UBP PF02148 126 - 187 8.3e-20 Zn-finger in ubiquitin-hydrolases and other protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 704
AccB1I GGYRCC 1 cut(s) 175
AccB7I CCANNNNNTGG 1 cut(s) 352
AccII CGCG 1 cut(s) 297
AciI CCGC 2 cut(s) 116, 295
AcsI RAATTY 1 cut(s) 394
AdeI CACNNNGTG 1 cut(s) 493
AfaI GTAC 1 cut(s) 621
AfiI CCNNNNNNNGG 3 cut(s) 185, 352, 562
AflII CTTAAG 1 cut(s) 703
AflIII ACRYGT 2 cut(s) 402, 577
AgsI TTSAA 5 cut(s) 47, 239, 305, 400, 479
AjnI CCWGG 2 cut(s) 133, 345
AluBI AGCT 1 cut(s) 208
AluI AGCT 1 cut(s) 208
ApoI RAATTY 1 cut(s) 394
AsuHPI GGTGA 2 cut(s) 206, 275
AsuII TTCGAA 2 cut(s) 8, 508
BaeGI GKGCMC 1 cut(s) 178
BanI GGYRCC 1 cut(s) 175
BccI CCATC 4 cut(s) 191, 254, 530, 565
BciT130I CCWGG 2 cut(s) 135, 347
BfmI CTRYAG 1 cut(s) 209
BfrI CTTAAG 1 cut(s) 703
BfuAI ACCTGC 1 cut(s) 704
BisI GCNGC 2 cut(s) 116, 295
BlsI GCNGC 2 cut(s) 117, 296
Bme1390I CCNGG 2 cut(s) 135, 347
BmiI GGNNCC 1 cut(s) 177
BmrFI CCNGG 2 cut(s) 135, 347
BmsI GCATC 3 cut(s) 322, 653, 708
Bpu14I TTCGAA 2 cut(s) 8, 508
BsaJI CCNNGG 2 cut(s) 436, 590
Bsc4I CCNNNNNNNGG 3 cut(s) 185, 352, 562
BseBI CCWGG 2 cut(s) 135, 347
BseDI CCNNGG 2 cut(s) 436, 590
BseGI GGATG 3 cut(s) 163, 262, 571
BseLI CCNNNNNNNGG 3 cut(s) 185, 352, 562
BseMII CTCAG 1 cut(s) 690
BseRI GAGGAG 2 cut(s) 176, 179
BseSI GKGCMC 1 cut(s) 178
Bsh1236I CGCG 1 cut(s) 297
BshNI GGYRCC 1 cut(s) 175
BsiSI CCGG 1 cut(s) 104
BslFI GGGAC 2 cut(s) 164, 439
BslI CCNNNNNNNGG 3 cut(s) 185, 352, 562
BsmFI GGGAC 2 cut(s) 164, 439
Bsp119I TTCGAA 2 cut(s) 8, 508
Bsp1286I GDGCHC 1 cut(s) 178
BspACI CCGC 2 cut(s) 116, 295
BspCNI CTCAG 1 cut(s) 689
BspFNI CGCG 1 cut(s) 297
BspLI GGNNCC 1 cut(s) 177
BspMI ACCTGC 1 cut(s) 704
BspT104I TTCGAA 2 cut(s) 8, 508
BspT107I GGYRCC 1 cut(s) 175
BspTI CTTAAG 1 cut(s) 703
BssECI CCNNGG 2 cut(s) 436, 590
BssT1I CCWWGG 2 cut(s) 436, 590
Bst2UI CCWGG 2 cut(s) 135, 347
Bst6I CTCTTC 4 cut(s) 5, 115, 246, 436
BstAFI CTTAAG 1 cut(s) 703
BstBI TTCGAA 2 cut(s) 8, 508
BstC8I GCNNGC 3 cut(s) 120, 380, 412
BstDEI CTNAG 1 cut(s) 676
BstF5I GGATG 3 cut(s) 163, 262, 571
BstFNI CGCG 1 cut(s) 297
BstNI CCWGG 2 cut(s) 135, 347
BstNSI RCATGY 2 cut(s) 414, 581
BstSCI CCNGG 2 cut(s) 133, 345
BstSFI CTRYAG 1 cut(s) 209
BstSLI GKGCMC 1 cut(s) 178
BstUI CGCG 1 cut(s) 297
BtsCI GGATG 3 cut(s) 163, 262, 571
BveI ACCTGC 1 cut(s) 704
Cac8I GCNNGC 3 cut(s) 120, 380, 412
Csp6I GTAC 1 cut(s) 620
CviAII CATG 4 cut(s) 20, 411, 491, 578
CviJI RGCY 4 cut(s) 208, 215, 294, 474
CviKI_1 RGCY 4 cut(s) 208, 215, 294, 474
CviQI GTAC 1 cut(s) 620
DdeI CTNAG 1 cut(s) 676
DraIII CACNNNGTG 1 cut(s) 493
Eam1104I CTCTTC 4 cut(s) 5, 115, 246, 436
EarI CTCTTC 4 cut(s) 5, 115, 246, 436
Eco130I CCWWGG 2 cut(s) 436, 590
EcoRII CCWGG 2 cut(s) 133, 345
EcoT14I CCWWGG 2 cut(s) 436, 590
EcoT22I ATGCAT 1 cut(s) 412
ErhI CCWWGG 2 cut(s) 436, 590
FaeI CATG 4 cut(s) 23, 414, 494, 581
FalI AAGNNNNNCTT 2 cut(s) 583, 615
FaqI GGGAC 2 cut(s) 164, 439
FatI CATG 4 cut(s) 19, 410, 490, 577
Fnu4HI GCNGC 2 cut(s) 116, 295
FokI GGATG 3 cut(s) 170, 269, 578
Fsp4HI GCNGC 2 cut(s) 116, 295
GluI GCNGC 2 cut(s) 116, 295
HapII CCGG 1 cut(s) 104
Hin1II CATG 4 cut(s) 23, 414, 494, 581
HinfI GANTC 1 cut(s) 107
HpaII CCGG 1 cut(s) 104
HphI GGTGA 2 cut(s) 206, 275
Hpy166II GTNNAC 2 cut(s) 266, 597
Hpy188I TCNGA 1 cut(s) 585
Hpy188III TCNNGA 2 cut(s) 329, 533
Hpy8I GTNNAC 2 cut(s) 266, 597
HpyAV CCTTC 8 cut(s) 41, 47, 137, 165, 441, 511, 539, 639
HpyCH4IV ACGT 1 cut(s) 402
HpyCH4V TGCA 4 cut(s) 410, 644, 691, 699
HpyF3I CTNAG 1 cut(s) 676
HpySE526I ACGT 1 cut(s) 402
Hsp92II CATG 4 cut(s) 23, 414, 494, 581
LmnI GCTCC 1 cut(s) 205
LweI GCATC 3 cut(s) 322, 653, 708
MaeII ACGT 1 cut(s) 402
MaeIII GTNAC 1 cut(s) 551
MhlI GDGCHC 1 cut(s) 178
MluCI AATT 4 cut(s) 142, 300, 394, 546
MlyI GAGTC 1 cut(s) 101
Mph1103I ATGCAT 1 cut(s) 412
MseI TTAA 4 cut(s) 141, 183, 339, 704
MspCI CTTAAG 1 cut(s) 703
MspI CCGG 1 cut(s) 104
MspR9I CCNGG 2 cut(s) 135, 347
MvaI CCWGG 2 cut(s) 135, 347
MvnI CGCG 1 cut(s) 297
NlaIII CATG 4 cut(s) 23, 414, 494, 581
NlaIV GGNNCC 1 cut(s) 177
NmuCI GTSAC 1 cut(s) 551
NsiI ATGCAT 1 cut(s) 412
NspI RCATGY 2 cut(s) 414, 581
NspV TTCGAA 2 cut(s) 8, 508
PaeI GCATGC 1 cut(s) 414
PciI ACATGT 1 cut(s) 577
PflMI CCANNNNNTGG 1 cut(s) 352
PkrI GCNGC 2 cut(s) 117, 296
PleI GAGTC 1 cut(s) 101
PpsI GAGTC 1 cut(s) 101
PscI ACATGT 1 cut(s) 577
Psp6I CCWGG 2 cut(s) 133, 345
PspGI CCWGG 2 cut(s) 133, 345
PspN4I GGNNCC 1 cut(s) 177
RsaI GTAC 1 cut(s) 621
RsaNI GTAC 1 cut(s) 620
SaqAI TTAA 4 cut(s) 141, 183, 339, 704
SatI GCNGC 2 cut(s) 116, 295
SchI GAGTC 1 cut(s) 101
ScrFI CCNGG 2 cut(s) 135, 347
SduI GDGCHC 1 cut(s) 178
SetI ASST 8 cut(s) 181, 210, 351, 405, 522, 596, 631, 698
SfaNI GCATC 3 cut(s) 322, 653, 708
SfcI CTRYAG 1 cut(s) 209
SfuI TTCGAA 2 cut(s) 8, 508
SmlI CTYRAG 1 cut(s) 703
SmoI CTYRAG 1 cut(s) 703
SphI GCATGC 1 cut(s) 414
Sse9I AATT 4 cut(s) 142, 300, 394, 546
SsiI CCGC 2 cut(s) 116, 295
StyD4I CCNGG 2 cut(s) 133, 345
StyI CCWWGG 2 cut(s) 436, 590
TaiI ACGT 1 cut(s) 405
TaqI TCGA 5 cut(s) 8, 232, 508, 574, 709
TasI AATT 4 cut(s) 142, 300, 394, 546
TatI WGTACW 1 cut(s) 619
TauI GCSGC 2 cut(s) 118, 297
Tru1I TTAA 4 cut(s) 141, 183, 339, 704
Tru9I TTAA 4 cut(s) 141, 183, 339, 704
TseFI GTSAC 1 cut(s) 551
Tsp45I GTSAC 1 cut(s) 551
TspDTI ATGAA 2 cut(s) 36, 558
Van91I CCANNNNNTGG 1 cut(s) 352
Vha464I CTTAAG 1 cut(s) 703
XapI RAATTY 1 cut(s) 394
XceI RCATGY 2 cut(s) 414, 581
Zsp2I ATGCAT 1 cut(s) 412
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.