RchiOBHm_Chr5g0001511

U4 U6.U5 tri-snRNP-associated protein 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0002617
Physical Location & Seq
Forward (+)
788975 .. 792249
3275 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ28293

Sequence Viewer

Length: 1038 bp
ATGCTTATTAGTCATGTTGAGCAGATTGACAAAAACAAGCAGTGGTCAAGGGCCCTTGATGGTTCTGATTACCTTCCTGGAATGGTGGGGCTAAATAACATTAAGGAGACTGATTTTGTGAATGTCACTATTCAATCCTTAATGAGAGTTATGCCTTTAAGAAACTTTTTTCTTATCCCAAAGAACTATCAACATTGCAAGTCTCCGCTTGTTCATCGATTTGGGGAGCTTACAAGAAAGATATGGCATGCACGCAACTTTAAAGGACAGGTGAGCCCGCATGAGTTCCTGCAGGCAGTTATGAAAGCCAGTAAGAAACGGTTTCGAATAGGAGCTCAGTCTGACCCTGTTGAGTTCATGTCATGGATGCTCAATACACTTCATGCAGATCTTAAAACTAAGAAGAATACCAGCATCATTTATGACTGCTTTCAGGGGGAACTAGAGGTTGTCAAGGAGACCCACAAGAATACAGATGGAGGAACTGAATACAAGGGCCTTGTCACAGAAACTTACAGAATGCCTTTCTTAATGCTTGGATTGGATTTGCCCCCACCACCTCTTTTTAAAGATGTGATGGAGAAAAACATCATACCACAGGTCCCGCTGTTCAACATTTTGAAGAAATTTGATGGTGAGACTGTTACTGAAATCGTCCGGCCTCGCATAGCAAGGACGAGGTATCGCGTTACTAGATTACCACGGTATCTAATTCTGCACATGCAACGATTCACAAAGAACAACTTCTTTGTGGAGAAGAATCCGACTCTAGTCAACTTTCCTGTGAAGAACCTGGAATTGAGAGATTACATTCCTTTGCCAACCCCCAAGGAGAATGAGAGATTGCGTTCAAAGTATGATTTGATTGCCAATATTGTTCATGATGGTAAACCTGGTGAAGGGTCCTACAGAGTATTTGTACAGCGAAAGTCAGAAGAACTATGGTATGAGATGCAGGATCTTCATGTCTCAGAAACACTTCCTCAGATGGTTGCTCTTTCCGAGGCTTATATGCAGATATATGAGCAGCAACAGTAA

Protein Analysis

345

Amino Acids

40.53

Weight (kDa)

9.37

Isoelectric Point (pI)

41.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UCH PF00443 29 - 341 9.2e-45 Ubiquitin carboxyl-terminal hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 687
AciI CCGC 3 cut(s) 206, 278, 605
AclWI GGATC 1 cut(s) 966
AcsI RAATTY 1 cut(s) 626
AfaI GTAC 1 cut(s) 921
AfiI CCNNNNNNNGG 1 cut(s) 899
AgsI TTSAA 4 cut(s) 134, 613, 622, 852
AjnI CCWGG 3 cut(s) 76, 792, 892
AluBI AGCT 2 cut(s) 229, 335
AluI AGCT 2 cut(s) 229, 335
Alw21I GWGCWC 1 cut(s) 337
Alw26I GTCTC 5 cut(s) 101, 207, 452, 632, 973
AlwI GGATC 1 cut(s) 966
AoxI GGCC 3 cut(s) 51, 496, 659
ApaI GGGCCC 1 cut(s) 55
ApeKI GCWGC 1 cut(s) 1027
ApoI RAATTY 1 cut(s) 626
Asp700I GAANNNNTTC 2 cut(s) 743, 978
AspS9I GGNCC 5 cut(s) 51, 52, 496, 601, 903
AsuHPI GGTGA 3 cut(s) 283, 647, 908
AsuII TTCGAA 1 cut(s) 325
AvaII GGWCC 2 cut(s) 601, 903
BaeGI GKGCMC 1 cut(s) 55
BaeI ACNNNNGTAYC 2 cut(s) 689, 722
BanII GRGCYC 3 cut(s) 55, 278, 337
Bbv12I GWGCWC 1 cut(s) 337
BccI CCATC 6 cut(s) 53, 470, 571, 626, 878, 982
BciT130I CCWGG 3 cut(s) 78, 794, 894
BcoDI GTCTC 5 cut(s) 101, 207, 452, 632, 973
BfaI CTAG 3 cut(s) 443, 693, 770
BfmI CTRYAG 2 cut(s) 290, 907
BglII AGATCT 1 cut(s) 388
BisI GCNGC 1 cut(s) 1028
BlsI GCNGC 1 cut(s) 1029
Bme1390I CCNGG 3 cut(s) 78, 794, 894
Bme18I GGWCC 2 cut(s) 601, 903
BmgT120I GGNCC 5 cut(s) 51, 52, 496, 601, 903
BmiI GGNNCC 3 cut(s) 53, 603, 904
BmrFI CCNGG 3 cut(s) 78, 794, 894
BmsI GCATC 3 cut(s) 357, 423, 942
BoxI GACNNNNGTC 1 cut(s) 770
Bpu14I TTCGAA 1 cut(s) 325
Bsa29I ATCGAT 1 cut(s) 217
BsaI GGTCTC 1 cut(s) 452
BsaJI CCNNGG 3 cut(s) 701, 828, 1002
Bsc4I CCNNNNNNNGG 1 cut(s) 899
Bse1I ACTGG 1 cut(s) 309
Bse3DI GCAATG 1 cut(s) 193
BseBI CCWGG 3 cut(s) 78, 794, 894
BseCI ATCGAT 1 cut(s) 217
BseDI CCNNGG 3 cut(s) 701, 828, 1002
BseGI GGATG 1 cut(s) 372
BseLI CCNNNNNNNGG 1 cut(s) 899
BseMI GCAATG 1 cut(s) 193
BseMII CTCAG 3 cut(s) 350, 984, 998
BseNI ACTGG 1 cut(s) 309
BseSI GKGCMC 1 cut(s) 55
BsgI GTGCAG 1 cut(s) 701
Bsh1236I CGCG 1 cut(s) 687
BshFI GGCC 3 cut(s) 53, 498, 661
BshVI ATCGAT 1 cut(s) 217
BsiHKAI GWGCWC 1 cut(s) 337
BsiSI CCGG 1 cut(s) 658
BslFI GGGAC 1 cut(s) 587
BslI CCNNNNNNNGG 1 cut(s) 899
BsmAI GTCTC 5 cut(s) 101, 207, 452, 632, 973
BsmFI GGGAC 1 cut(s) 587
BsmI GAATGC 1 cut(s) 525
BsnI GGCC 3 cut(s) 53, 498, 661
Bso31I GGTCTC 1 cut(s) 452
Bsp119I TTCGAA 1 cut(s) 325
Bsp120I GGGCCC 1 cut(s) 51
Bsp1286I GDGCHC 3 cut(s) 55, 278, 337
Bsp1407I TGTACA 1 cut(s) 919
Bsp143I GATC 2 cut(s) 388, 958
BspACI CCGC 3 cut(s) 206, 278, 605
BspANI GGCC 3 cut(s) 53, 498, 661
BspCNI CTCAG 3 cut(s) 349, 983, 997
BspDI ATCGAT 1 cut(s) 217
BspFNI CGCG 1 cut(s) 687
BspHI TCATGA 1 cut(s) 880
BspLI GGNNCC 3 cut(s) 53, 603, 904
BspMAI CTGCAG 1 cut(s) 294
BspPI GGATC 1 cut(s) 966
BspT104I TTCGAA 1 cut(s) 325
BspTNI GGTCTC 1 cut(s) 452
BsrDI GCAATG 1 cut(s) 193
BsrGI TGTACA 1 cut(s) 919
BsrI ACTGG 1 cut(s) 309
BssECI CCNNGG 3 cut(s) 701, 828, 1002
BssMI GATC 2 cut(s) 388, 958
BssT1I CCWWGG 1 cut(s) 828
Bst2UI CCWGG 3 cut(s) 78, 794, 894
Bst4CI ACNGT 4 cut(s) 321, 643, 705, 1035
BstAUI TGTACA 1 cut(s) 919
BstBI TTCGAA 1 cut(s) 325
BstC8I GCNNGC 4 cut(s) 249, 253, 278, 294
BstDEI CTNAG 4 cut(s) 336, 399, 970, 984
BstDSI CCRYGG 1 cut(s) 701
BstENI CCTNNNNNAGG 1 cut(s) 897
BstF5I GGATG 1 cut(s) 372
BstFNI CGCG 1 cut(s) 687
BstKTI GATC 2 cut(s) 391, 961
BstMAI GTCTC 5 cut(s) 101, 207, 452, 632, 973
BstMBI GATC 2 cut(s) 388, 958
BstNI CCWGG 3 cut(s) 78, 794, 894
BstNSI RCATGY 2 cut(s) 251, 724
BstPAI GACNNNNGTC 1 cut(s) 770
BstSCI CCNGG 3 cut(s) 76, 792, 892
BstSFI CTRYAG 2 cut(s) 290, 907
BstSLI GKGCMC 1 cut(s) 55
BstUI CGCG 1 cut(s) 687
BstX2I RGATCY 2 cut(s) 388, 958
BstYI RGATCY 2 cut(s) 388, 958
Bsu15I ATCGAT 1 cut(s) 217
BsuRI GGCC 3 cut(s) 53, 498, 661
BsuTUI ATCGAT 1 cut(s) 217
BtgI CCRYGG 1 cut(s) 701
BtsCI GGATG 1 cut(s) 372
BtsI GCAGTG 1 cut(s) 47
BtsIMutI CAGTG 1 cut(s) 47
Cac8I GCNNGC 4 cut(s) 249, 253, 278, 294
CciI TCATGA 1 cut(s) 880
Cfr13I GGNCC 5 cut(s) 51, 52, 496, 601, 903
ClaI ATCGAT 1 cut(s) 217
CsiI ACCWGGT 1 cut(s) 892
Csp6I GTAC 1 cut(s) 920
CviAII CATG 9 cut(s) 14, 248, 281, 358, 363, 383, 721, 881, 965
CviJI RGCY 9 cut(s) 53, 91, 229, 276, 308, 335, 498, 661, 1007
CviKI_1 RGCY 9 cut(s) 53, 91, 229, 276, 308, 335, 498, 661, 1007
CviQI GTAC 1 cut(s) 920
DdeI CTNAG 4 cut(s) 336, 399, 970, 984
DpnI GATC 2 cut(s) 390, 960
DpnII GATC 2 cut(s) 388, 958
DraI TTTAAA 2 cut(s) 262, 568
Ecl136II GAGCTC 1 cut(s) 335
Eco130I CCWWGG 1 cut(s) 828
Eco24I GRGCYC 3 cut(s) 55, 278, 337
Eco31I GGTCTC 1 cut(s) 452
Eco47I GGWCC 2 cut(s) 601, 903
Eco53kI GAGCTC 1 cut(s) 335
EcoICRI GAGCTC 1 cut(s) 335
EcoNI CCTNNNNNAGG 1 cut(s) 897
EcoO109I RGGNCCY 5 cut(s) 51, 52, 496, 601, 903
EcoRII CCWGG 3 cut(s) 76, 792, 892
EcoT14I CCWWGG 1 cut(s) 828
EcoT38I GRGCYC 3 cut(s) 55, 278, 337
ErhI CCWWGG 1 cut(s) 828
FaeI CATG 9 cut(s) 17, 251, 284, 361, 366, 386, 724, 884, 968
FalI AAGNNNNNCTT 2 cut(s) 728, 760
FaqI GGGAC 1 cut(s) 587
FatI CATG 9 cut(s) 13, 247, 280, 357, 362, 382, 720, 880, 964
FauI CCCGC 2 cut(s) 285, 612
Fnu4HI GCNGC 1 cut(s) 1028
FokI GGATG 1 cut(s) 379
FriOI GRGCYC 3 cut(s) 55, 278, 337
Fsp4HI GCNGC 1 cut(s) 1028
FspBI CTAG 3 cut(s) 443, 693, 770
GluI GCNGC 1 cut(s) 1028
HaeIII GGCC 3 cut(s) 53, 498, 661
HapII CCGG 1 cut(s) 658
Hin1II CATG 9 cut(s) 17, 251, 284, 361, 366, 386, 724, 884, 968
HincII GTYRAC 1 cut(s) 775
HindII GTYRAC 1 cut(s) 775
HinfI GANTC 3 cut(s) 729, 760, 766
HpaII CCGG 1 cut(s) 658
HphI GGTGA 3 cut(s) 283, 647, 908
Hpy166II GTNNAC 2 cut(s) 775, 890
Hpy188I TCNGA 7 cut(s) 67, 343, 765, 934, 973, 987, 1003
Hpy188III TCNNGA 1 cut(s) 881
Hpy8I GTNNAC 2 cut(s) 775, 890
HpyAV CCTTC 2 cut(s) 83, 893
HpyCH4III ACNGT 4 cut(s) 321, 643, 705, 1035
HpyCH4V TGCA 8 cut(s) 198, 251, 292, 386, 718, 724, 955, 1015
HpyF3I CTNAG 4 cut(s) 336, 399, 970, 984
Hsp92II CATG 9 cut(s) 17, 251, 284, 361, 366, 386, 724, 884, 968
Kzo9I GATC 2 cut(s) 388, 958
LmnI GCTCC 2 cut(s) 226, 332
LweI GCATC 3 cut(s) 357, 423, 942
MabI ACCWGGT 1 cut(s) 892
MaeI CTAG 3 cut(s) 443, 693, 770
MaeIII GTNAC 4 cut(s) 124, 502, 643, 688
MalI GATC 2 cut(s) 390, 960
MboI GATC 2 cut(s) 388, 958
MboII GAAGA 6 cut(s) 415, 634, 769, 799, 947, 953
MflI RGATCY 2 cut(s) 388, 958
MhlI GDGCHC 3 cut(s) 55, 278, 337
MluCI AATT 3 cut(s) 626, 711, 797
MlyI GAGTC 1 cut(s) 760
MmeI TCCRAC 1 cut(s) 788
MnlI CCTC 7 cut(s) 439, 473, 570, 672, 672, 993, 997
MroXI GAANNNNTTC 2 cut(s) 743, 978
MseI TTAA 7 cut(s) 102, 140, 158, 261, 393, 530, 567
MspA1I CMGCKG 1 cut(s) 607
MspI CCGG 1 cut(s) 658
MspR9I CCNGG 3 cut(s) 78, 794, 894
Mva1269I GAATGC 1 cut(s) 525
MvaI CCWGG 3 cut(s) 78, 794, 894
MvnI CGCG 1 cut(s) 687
NdeII GATC 2 cut(s) 388, 958
NlaIII CATG 9 cut(s) 17, 251, 284, 361, 366, 386, 724, 884, 968
NlaIV GGNNCC 3 cut(s) 53, 603, 904
NmuCI GTSAC 2 cut(s) 124, 502
NspI RCATGY 2 cut(s) 251, 724
NspV TTCGAA 1 cut(s) 325
PaeI GCATGC 1 cut(s) 251
PagI TCATGA 1 cut(s) 880
PctI GAATGC 1 cut(s) 525
PdmI GAANNNNTTC 2 cut(s) 743, 978
PfeI GAWTC 2 cut(s) 729, 760
PfoI TCCNGGA 1 cut(s) 76
PkrI GCNGC 1 cut(s) 1029
PleI GAGTC 1 cut(s) 760
PpsI GAGTC 1 cut(s) 760
PpuMI RGGWCCY 2 cut(s) 601, 903
PshAI GACNNNNGTC 1 cut(s) 770
Psp124BI GAGCTC 1 cut(s) 337
Psp5II RGGWCCY 2 cut(s) 601, 903
Psp6I CCWGG 3 cut(s) 76, 792, 892
PspGI CCWGG 3 cut(s) 76, 792, 892
PspN4I GGNNCC 3 cut(s) 53, 603, 904
PspOMI GGGCCC 1 cut(s) 51
PspPI GGNCC 5 cut(s) 51, 52, 496, 601, 903
PspPPI RGGWCCY 2 cut(s) 601, 903
PstI CTGCAG 1 cut(s) 294
PsuI RGATCY 2 cut(s) 388, 958
RsaI GTAC 1 cut(s) 921
RsaNI GTAC 1 cut(s) 920
SacI GAGCTC 1 cut(s) 337
SaqAI TTAA 7 cut(s) 102, 140, 158, 261, 393, 530, 567
SatI GCNGC 1 cut(s) 1028
Sau3AI GATC 2 cut(s) 388, 958
Sau96I GGNCC 5 cut(s) 51, 52, 496, 601, 903
SbfI CCTGCAGG 1 cut(s) 294
SchI GAGTC 1 cut(s) 760
ScrFI CCNGG 3 cut(s) 78, 794, 894
SdaI CCTGCAGG 1 cut(s) 294
SduI GDGCHC 3 cut(s) 55, 278, 337
SexAI ACCWGGT 1 cut(s) 892
SfaNI GCATC 3 cut(s) 357, 423, 942
SfcI CTRYAG 2 cut(s) 290, 907
SfuI TTCGAA 1 cut(s) 325
SinI GGWCC 2 cut(s) 601, 903
SphI GCATGC 1 cut(s) 251
Sse8387I CCTGCAGG 1 cut(s) 294
Sse9I AATT 3 cut(s) 626, 711, 797
SsiI CCGC 3 cut(s) 206, 278, 605
SspI AATATT 1 cut(s) 874
SspMI CTAG 3 cut(s) 443, 693, 770
SstI GAGCTC 1 cut(s) 337
StyD4I CCNGG 3 cut(s) 76, 792, 892
StyI CCWWGG 1 cut(s) 828
TaaI ACNGT 4 cut(s) 321, 643, 705, 1035
TaqI TCGA 2 cut(s) 217, 325
TasI AATT 3 cut(s) 626, 711, 797
TatI WGTACW 1 cut(s) 919
TfiI GAWTC 2 cut(s) 729, 760
Tru1I TTAA 7 cut(s) 102, 140, 158, 261, 393, 530, 567
Tru9I TTAA 7 cut(s) 102, 140, 158, 261, 393, 530, 567
TscAI CASTG 1 cut(s) 47
TseFI GTSAC 2 cut(s) 124, 502
TseI GCWGC 1 cut(s) 1027
Tsp45I GTSAC 2 cut(s) 124, 502
TspDTI ATGAA 6 cut(s) 203, 317, 346, 371, 869, 953
TspRI CASTG 1 cut(s) 47
VpaK11BI GGWCC 2 cut(s) 601, 903
XagI CCTNNNNNAGG 1 cut(s) 897
XapI RAATTY 1 cut(s) 626
XceI RCATGY 2 cut(s) 251, 724
XmnI GAANNNNTTC 2 cut(s) 743, 978
XspI CTAG 3 cut(s) 443, 693, 770
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.