AT4G23330

BLOC-1-related complex sub-unit 6

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0006371
Physical Location & Seq
Forward (+)
12191851 .. 12195159
3309 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G23330.3

Sequence Viewer

Length: 813 bp
ATGGATGCCGGAGCTGAAATTGATGTGGTTCGGCCGGTGGAGGCAGATTCGGCGGCGGAAATTAGTCCGGAACAAGACCACGAAGGAAGATCGGAGCCTTCGTCGTTGAACCAAGGGGAGATTTTAAAAACACTTGCTACGGTGGAGAAGGATTCGCAGGCAATCGCGGAGAGCTTTTCGTCTCTCTTTGTTTCCCTCCGATCAACTCTTTCTGAGGCTACTGGTAGCTCGGTTGATCACATGGCTTGCTTTGGAGAGGCAGCGGGACGGCTTCAGGAAAATGCTCTAGATGCAGCCACCAAAGGGAACCGGTACATCAACTCTTGCCTCAGACTTTGGATTACGTATTTCTCGTACATAAAGATTTTGATTATAAAACTGATGATGCCAAAAAATTCTAAACTTACGTTTCGCTCCCTCTCTAAGTCCCTCTCGCTCTTTGGTTCTTCTTCTCCCGCAAGATTGAATCTTTGGTTTCTTTGGATTGGTTTCGTAAGTGTTCGCACCGAATCATCAACCATTTCACCGCTGGCGGATGCTCTGGAGGAGGAGGCCCTTGATGTCGATGACTTTGAAGCAGATAAAAAGCCTGAAGACTTGCAGCCAAGTGTTGTATATCAGCAACGACTGCTAGAGGAAAGGAAAATTTATGAAAATGAGCAACAGCGTGAGGTTGAGCGTCTTAAGAGTGACCTGAAGGAGAAGAAAAGGTTGGCCAGAATGTTGGATAATAAGGAAATATTCAGGGTGATTCGTCTTCGACAACCTGAGTTTGAGAGGCTAAGAAGCGAGAGGAGCATATTGGTCAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BORCS6

Protein Analysis

270

Amino Acids

30.67

Weight (kDa)

5.3

Isoelectric Point (pI)

62.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BORCS6 PF10157 43 - 137 1.4e-20 BLOC-1-related complex sub-unit 6 C-terminal helix
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 374
AccII CGCG 1 cut(s) 167
AccIII TCCGGA 1 cut(s) 67
AciI CCGC 7 cut(s) 53, 56, 167, 263, 456, 527, 533
AcoI YGGCCR 2 cut(s) 32, 714
AcsI RAATTY 2 cut(s) 394, 645
AcuI CTGAAG 3 cut(s) 257, 612, 716
AfaI GTAC 2 cut(s) 314, 356
AfiI CCNNNNNNNGG 1 cut(s) 303
AflII CTTAAG 1 cut(s) 683
AgeI ACCGGT 1 cut(s) 309
AgsI TTSAA 3 cut(s) 109, 466, 575
AjuI GAANNNNNNNTTGG 2 cut(s) 695, 727
AluBI AGCT 3 cut(s) 14, 174, 228
AluI AGCT 3 cut(s) 14, 174, 228
Alw26I GTCTC 1 cut(s) 186
Aor13HI TCCGGA 1 cut(s) 67
AoxI GGCC 3 cut(s) 32, 552, 714
ApeKI GCWGC 3 cut(s) 260, 293, 601
ApoI RAATTY 2 cut(s) 394, 645
AsiGI ACCGGT 1 cut(s) 309
AspS9I GGNCC 1 cut(s) 553
AsuHPI GGTGA 2 cut(s) 516, 760
BalI TGGCCA 1 cut(s) 716
BbsI GAAGAC 2 cut(s) 600, 749
BbvI GCAGC 3 cut(s) 272, 305, 613
BceAI ACGGC 1 cut(s) 284
BclI TGATCA 1 cut(s) 235
BcoDI GTCTC 1 cut(s) 186
BfaI CTAG 2 cut(s) 287, 632
BfrI CTTAAG 1 cut(s) 683
BisI GCNGC 4 cut(s) 54, 261, 294, 602
BlsI GCNGC 4 cut(s) 55, 262, 295, 603
BmgT120I GGNCC 1 cut(s) 553
BmiI GGNNCC 2 cut(s) 96, 308
BmsI GCATC 3 cut(s) 280, 375, 526
BpiI GAAGAC 2 cut(s) 600, 749
BpmI CTGGAG 1 cut(s) 563
BsaAI YACGTR 1 cut(s) 345
BsaJI CCNNGG 1 cut(s) 112
BsaWI WCCGGW 2 cut(s) 67, 309
BsaXI ACNNNNNCTCC 2 cut(s) 86, 116
Bsc4I CCNNNNNNNGG 1 cut(s) 303
Bse118I RCCGGY 2 cut(s) 34, 309
Bse1I ACTGG 1 cut(s) 226
BseAI TCCGGA 1 cut(s) 67
BseDI CCNNGG 1 cut(s) 112
BseGI GGATG 2 cut(s) 10, 541
BseLI CCNNNNNNNGG 1 cut(s) 303
BseMII CTCAG 3 cut(s) 204, 343, 759
BseNI ACTGG 1 cut(s) 226
BseRI GAGGAG 3 cut(s) 560, 563, 808
BseX3I CGGCCG 1 cut(s) 32
BseXI GCAGC 3 cut(s) 272, 305, 613
Bsh1236I CGCG 1 cut(s) 167
Bsh1285I CGRYCG 1 cut(s) 35
BshFI GGCC 3 cut(s) 34, 554, 716
BshTI ACCGGT 1 cut(s) 309
BsiEI CGRYCG 1 cut(s) 35
BsiSI CCGG 4 cut(s) 9, 35, 68, 310
BslFI GGGAC 2 cut(s) 279, 412
BslI CCNNNNNNNGG 1 cut(s) 303
BsmAI GTCTC 1 cut(s) 186
BsmBI CGTCTC 1 cut(s) 186
BsmFI GGGAC 2 cut(s) 279, 412
BsnI GGCC 3 cut(s) 34, 554, 716
Bsp13I TCCGGA 1 cut(s) 67
Bsp143I GATC 3 cut(s) 89, 200, 235
BspACI CCGC 7 cut(s) 53, 56, 167, 263, 456, 527, 533
BspANI GGCC 3 cut(s) 34, 554, 716
BspCNI CTCAG 3 cut(s) 205, 342, 760
BspEI TCCGGA 1 cut(s) 67
BspFNI CGCG 1 cut(s) 167
BspLI GGNNCC 2 cut(s) 96, 308
BspTI CTTAAG 1 cut(s) 683
BsrFI RCCGGY 2 cut(s) 34, 309
BsrI ACTGG 1 cut(s) 226
BssAI RCCGGY 2 cut(s) 34, 309
BssECI CCNNGG 1 cut(s) 112
BssMI GATC 3 cut(s) 89, 200, 235
BssT1I CCWWGG 1 cut(s) 112
Bst4CI ACNGT 1 cut(s) 142
BstAFI CTTAAG 1 cut(s) 683
BstAPI GCANNNNNTGC 1 cut(s) 628
BstBAI YACGTR 1 cut(s) 345
BstC8I GCNNGC 3 cut(s) 159, 247, 531
BstDEI CTNAG 5 cut(s) 213, 329, 423, 768, 782
BstF5I GGATG 2 cut(s) 10, 541
BstFNI CGCG 1 cut(s) 167
BstKTI GATC 3 cut(s) 92, 203, 238
BstMAI GTCTC 1 cut(s) 186
BstMBI GATC 3 cut(s) 89, 200, 235
BstMCI CGRYCG 1 cut(s) 35
BstMWI GCNNNNNNNGC 4 cut(s) 50, 290, 628, 795
BstSNI TACGTA 1 cut(s) 345
BstUI CGCG 1 cut(s) 167
BstV1I GCAGC 3 cut(s) 272, 305, 613
BstV2I GAAGAC 2 cut(s) 600, 749
BstXI CCANNNNNNTGG 1 cut(s) 724
BstZI CGGCCG 1 cut(s) 32
BsuRI GGCC 3 cut(s) 34, 554, 716
BtsCI GGATG 2 cut(s) 10, 541
Cac8I GCNNGC 3 cut(s) 159, 247, 531
Cfr10I RCCGGY 2 cut(s) 34, 309
Cfr13I GGNCC 1 cut(s) 553
CseI GACGC 1 cut(s) 668
Csp6I GTAC 2 cut(s) 313, 355
CspAI ACCGGT 1 cut(s) 309
CviAII CATG 1 cut(s) 241
CviQI GTAC 2 cut(s) 313, 355
DdeI CTNAG 5 cut(s) 213, 329, 423, 768, 782
DpnI GATC 3 cut(s) 91, 202, 237
DpnII GATC 3 cut(s) 89, 200, 235
DraI TTTAAA 1 cut(s) 126
EaeI YGGCCR 2 cut(s) 32, 714
EagI CGGCCG 1 cut(s) 32
EciI GGCGGA 2 cut(s) 71, 548
EclXI CGGCCG 1 cut(s) 32
Eco105I TACGTA 1 cut(s) 345
Eco130I CCWWGG 1 cut(s) 112
Eco52I CGGCCG 1 cut(s) 32
Eco57I CTGAAG 3 cut(s) 257, 612, 716
EcoO109I RGGNCCY 1 cut(s) 553
EcoT14I CCWWGG 1 cut(s) 112
ErhI CCWWGG 1 cut(s) 112
Esp3I CGTCTC 1 cut(s) 186
FaeI CATG 1 cut(s) 244
FaiI YATR 6 cut(s) 242, 359, 374, 616, 651, 800
FaqI GGGAC 2 cut(s) 279, 412
FatI CATG 1 cut(s) 240
FauI CCCGC 2 cut(s) 256, 463
FbaI TGATCA 1 cut(s) 235
Fnu4HI GCNGC 4 cut(s) 54, 261, 294, 602
FokI GGATG 2 cut(s) 17, 548
Fsp4HI GCNGC 4 cut(s) 54, 261, 294, 602
FspBI CTAG 2 cut(s) 287, 632
GluI GCNGC 4 cut(s) 54, 261, 294, 602
GsuI CTGGAG 1 cut(s) 563
HaeIII GGCC 3 cut(s) 34, 554, 716
HapII CCGG 4 cut(s) 9, 35, 68, 310
HgaI GACGC 1 cut(s) 668
Hin1II CATG 1 cut(s) 244
HinfI GANTC 5 cut(s) 47, 152, 466, 509, 751
HpaII CCGG 4 cut(s) 9, 35, 68, 310
HphI GGTGA 2 cut(s) 516, 760
Hpy188I TCNGA 4 cut(s) 94, 200, 214, 332
Hpy188III TCNNGA 4 cut(s) 68, 275, 287, 542
Hpy99I CGWCG 1 cut(s) 106
HpyAV CCTTC 4 cut(s) 77, 108, 142, 691
HpyCH4III ACNGT 1 cut(s) 142
HpyCH4IV ACGT 2 cut(s) 344, 407
HpyCH4V TGCA 2 cut(s) 293, 601
HpyF10VI GCNNNNNNNGC 4 cut(s) 50, 290, 628, 795
HpyF3I CTNAG 5 cut(s) 213, 329, 423, 768, 782
HpySE526I ACGT 2 cut(s) 344, 407
Hsp92II CATG 1 cut(s) 244
Kpn2I TCCGGA 1 cut(s) 67
Ksp22I TGATCA 1 cut(s) 235
Kzo9I GATC 3 cut(s) 89, 200, 235
LmnI GCTCC 4 cut(s) 11, 94, 419, 795
Lsp1109I GCAGC 3 cut(s) 272, 305, 613
LweI GCATC 3 cut(s) 280, 375, 526
MaeI CTAG 2 cut(s) 287, 632
MaeII ACGT 2 cut(s) 344, 407
MaeIII GTNAC 1 cut(s) 689
MalI GATC 3 cut(s) 91, 202, 237
MboI GATC 3 cut(s) 89, 200, 235
MboII GAAGA 6 cut(s) 99, 438, 441, 605, 715, 749
MlsI TGGCCA 1 cut(s) 716
MluCI AATT 4 cut(s) 18, 60, 394, 645
MluNI TGGCCA 1 cut(s) 716
MmeI TCCRAC 1 cut(s) 705
Mox20I TGGCCA 1 cut(s) 716
MroI TCCGGA 1 cut(s) 67
MscI TGGCCA 1 cut(s) 716
MseI TTAA 2 cut(s) 125, 684
Msp20I TGGCCA 1 cut(s) 716
MspA1I CMGCKG 2 cut(s) 263, 529
MspCI CTTAAG 1 cut(s) 683
MspI CCGG 4 cut(s) 9, 35, 68, 310
MvnI CGCG 1 cut(s) 167
MwoI GCNNNNNNNGC 4 cut(s) 50, 290, 628, 795
NdeII GATC 3 cut(s) 89, 200, 235
NlaIII CATG 1 cut(s) 244
NlaIV GGNNCC 2 cut(s) 96, 308
NmuCI GTSAC 1 cut(s) 689
PcsI WCGNNNNNNNCGW 2 cut(s) 98, 350
PfeI GAWTC 5 cut(s) 47, 152, 466, 509, 751
PinAI ACCGGT 1 cut(s) 309
PkrI GCNGC 4 cut(s) 55, 262, 295, 603
Ppu21I YACGTR 1 cut(s) 345
PsiI TTATAA 1 cut(s) 374
PspN4I GGNNCC 2 cut(s) 96, 308
PspPI GGNCC 1 cut(s) 553
RsaI GTAC 2 cut(s) 314, 356
RsaNI GTAC 2 cut(s) 313, 355
SaqAI TTAA 2 cut(s) 125, 684
SatI GCNGC 4 cut(s) 54, 261, 294, 602
Sau3AI GATC 3 cut(s) 89, 200, 235
Sau96I GGNCC 1 cut(s) 553
SetI ASST 9 cut(s) 16, 176, 230, 347, 410, 675, 696, 713, 769
SfaNI GCATC 3 cut(s) 280, 375, 526
SmlI CTYRAG 1 cut(s) 683
SmoI CTYRAG 1 cut(s) 683
SnaBI TACGTA 1 cut(s) 345
Sse9I AATT 4 cut(s) 18, 60, 394, 645
SsiI CCGC 7 cut(s) 53, 56, 167, 263, 456, 527, 533
SspI AATATT 1 cut(s) 741
SspMI CTAG 2 cut(s) 287, 632
StyI CCWWGG 1 cut(s) 112
TaaI ACNGT 1 cut(s) 142
TaiI ACGT 2 cut(s) 347, 410
TaqI TCGA 2 cut(s) 564, 760
TasI AATT 4 cut(s) 18, 60, 394, 645
TauI GCSGC 1 cut(s) 56
TfiI GAWTC 5 cut(s) 47, 152, 466, 509, 751
Tru1I TTAA 2 cut(s) 125, 684
Tru9I TTAA 2 cut(s) 125, 684
TseFI GTSAC 1 cut(s) 689
TseI GCWGC 3 cut(s) 260, 293, 601
Tsp45I GTSAC 1 cut(s) 689
TspDTI ATGAA 1 cut(s) 666
Vha464I CTTAAG 1 cut(s) 683
XapI RAATTY 2 cut(s) 394, 645
XbaI TCTAGA 1 cut(s) 286
XcmI CCANNNNNNNNNTGG 1 cut(s) 526
XspI CTAG 2 cut(s) 287, 632
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.