RLG00000003926

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0006371
Physical Location & Seq
Forward (+)
54347440 .. 54348994
1555 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003926

Sequence Viewer

Length: 489 bp
ATGGAGGAAGACGGAGGAGATCAAGCTCCGCTGCCGCATTCGCCGAGCTCCGAAACCCCAATCTCCGACCCAAACCTCGATCCACCGTCCACGTGTCCCGATTCCACACTCGCTCATACTCAGGACGACGGCTCCGACTCAAAACCCCAGCTGAACCAGACCGATCTATTCAGAGCTCTCGAAGTCGTTGAGAGGGACTCCCTCGCCATCGCGGAGAGCTTCACCTCTCTCTTCTCCTCCCTCCGCCTCGCCCTCTCCGAGGTTACTAGCAACTCGGTTGATCACATGCATTGCTTTGGCGACGCCGCCGGTCGGCTTCAAGAATCCGTGCTGGATGCTGCTACTAAGGGGAATCGGTACATAAATTCATGTCTGAGATTAAATGAAGAAATGAAGGGTGTTGATAGTCTAGCCCTGCAGCTAAAAATCTTGAGAAGAAATGTTGATGCTCTAGACTCCGGAGTTAACAGGCTCCTTCGCCTTCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BORCS6

Protein Analysis

163

Amino Acids

17.59

Weight (kDa)

4.57

Isoelectric Point (pI)

55.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BORCS6 PF10157 58 - 152 1.4e-20 BLOC-1-related complex sub-unit 6 C-terminal helix
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 212
AccIII TCCGGA 1 cut(s) 458
AciI CCGC 5 cut(s) 29, 35, 212, 244, 306
AclWI GGATC 1 cut(s) 74
AcsI RAATTY 1 cut(s) 364
AcvI CACGTG 1 cut(s) 93
AcyI GRCGYC 1 cut(s) 303
AfaI GTAC 1 cut(s) 359
AfiI CCNNNNNNNGG 2 cut(s) 259, 312
AflIII ACRYGT 1 cut(s) 92
AgsI TTSAA 1 cut(s) 320
AluBI AGCT 6 cut(s) 26, 48, 151, 176, 219, 421
AluI AGCT 6 cut(s) 26, 48, 151, 176, 219, 421
Alw21I GWGCWC 2 cut(s) 50, 178
AlwI GGATC 1 cut(s) 74
Aor13HI TCCGGA 1 cut(s) 458
ApeKI GCWGC 3 cut(s) 31, 338, 418
ApoI RAATTY 1 cut(s) 364
AsuHPI GGTGA 1 cut(s) 214
BanII GRGCYC 2 cut(s) 50, 178
BbrPI CACGTG 1 cut(s) 93
BbsI GAAGAC 1 cut(s) 15
Bbv12I GWGCWC 2 cut(s) 50, 178
BbvI GCAGC 3 cut(s) 18, 325, 430
BccI CCATC 1 cut(s) 215
BceAI ACGGC 1 cut(s) 145
BclI TGATCA 1 cut(s) 280
BfaI CTAG 3 cut(s) 267, 410, 452
BfmI CTRYAG 1 cut(s) 416
BisI GCNGC 5 cut(s) 32, 35, 306, 339, 419
BlsI GCNGC 5 cut(s) 33, 36, 307, 340, 420
BmiI GGNNCC 2 cut(s) 133, 473
BmsI GCATC 2 cut(s) 325, 436
BpiI GAAGAC 1 cut(s) 15
BplI GAGNNNNNCTC 2 cut(s) 182, 214
BpuEI CTTGAG 1 cut(s) 451
BsaAI YACGTR 1 cut(s) 93
BsaHI GRCGYC 1 cut(s) 303
BsaJI CCNNGG 1 cut(s) 258
BsaWI WCCGGW 1 cut(s) 458
BsaXI ACNNNNNCTCC 2 cut(s) 116, 146
Bsc4I CCNNNNNNNGG 2 cut(s) 259, 312
Bse118I RCCGGY 1 cut(s) 308
Bse3DI GCAATG 1 cut(s) 289
BseAI TCCGGA 1 cut(s) 458
BseDI CCNNGG 1 cut(s) 258
BseGI GGATG 1 cut(s) 340
BseLI CCNNNNNNNGG 2 cut(s) 259, 312
BseMI GCAATG 1 cut(s) 289
BseMII CTCAG 2 cut(s) 134, 365
BseRI GAGGAG 2 cut(s) 30, 226
BseXI GCAGC 3 cut(s) 18, 325, 430
BseYI CCCAGC 1 cut(s) 147
Bsh1236I CGCG 1 cut(s) 212
Bsh1285I CGRYCG 1 cut(s) 313
BsiEI CGRYCG 1 cut(s) 313
BsiHKAI GWGCWC 2 cut(s) 50, 178
BsiSI CCGG 2 cut(s) 309, 459
BslFI GGGAC 2 cut(s) 81, 209
BslI CCNNNNNNNGG 2 cut(s) 259, 312
BsmFI GGGAC 2 cut(s) 81, 209
BsmI GAATGC 1 cut(s) 37
Bsp1286I GDGCHC 2 cut(s) 50, 178
Bsp13I TCCGGA 1 cut(s) 458
Bsp143I GATC 4 cut(s) 19, 79, 163, 280
BspACI CCGC 5 cut(s) 29, 35, 212, 244, 306
BspCNI CTCAG 2 cut(s) 133, 366
BspEI TCCGGA 1 cut(s) 458
BspFNI CGCG 1 cut(s) 212
BspLI GGNNCC 2 cut(s) 133, 473
BspMAI CTGCAG 1 cut(s) 420
BspPI GGATC 1 cut(s) 74
BsrDI GCAATG 1 cut(s) 289
BsrFI RCCGGY 1 cut(s) 308
BssAI RCCGGY 1 cut(s) 308
BssECI CCNNGG 1 cut(s) 258
BssMI GATC 4 cut(s) 19, 79, 163, 280
BssNI GRCGYC 1 cut(s) 303
Bst4CI ACNGT 1 cut(s) 87
Bst6I CTCTTC 1 cut(s) 236
BstACI GRCGYC 1 cut(s) 303
BstBAI YACGTR 1 cut(s) 93
BstDEI CTNAG 3 cut(s) 120, 345, 374
BstENI CCTNNNNNAGG 1 cut(s) 257
BstF5I GGATG 1 cut(s) 340
BstFNI CGCG 1 cut(s) 212
BstKTI GATC 4 cut(s) 22, 82, 166, 283
BstMBI GATC 4 cut(s) 19, 79, 163, 280
BstMCI CGRYCG 1 cut(s) 313
BstMWI GCNNNNNNNGC 1 cut(s) 40
BstNSI RCATGY 1 cut(s) 289
BstSFI CTRYAG 1 cut(s) 416
BstUI CGCG 1 cut(s) 212
BstV1I GCAGC 3 cut(s) 18, 325, 430
BstV2I GAAGAC 1 cut(s) 15
BtgZI GCGATG 1 cut(s) 193
BtsCI GGATG 1 cut(s) 340
Cfr10I RCCGGY 1 cut(s) 308
CseI GACGC 1 cut(s) 311
Csp6I GTAC 1 cut(s) 358
CviAII CATG 3 cut(s) 286, 369, 486
CviQI GTAC 1 cut(s) 358
DdeI CTNAG 3 cut(s) 120, 345, 374
DpnI GATC 4 cut(s) 21, 81, 165, 282
DpnII GATC 4 cut(s) 19, 79, 163, 280
Eam1104I CTCTTC 1 cut(s) 236
EarI CTCTTC 1 cut(s) 236
EciI GGCGGA 1 cut(s) 233
Ecl136II GAGCTC 2 cut(s) 48, 176
Eco24I GRGCYC 2 cut(s) 50, 178
Eco53kI GAGCTC 2 cut(s) 48, 176
Eco72I CACGTG 1 cut(s) 93
EcoICRI GAGCTC 2 cut(s) 48, 176
EcoNI CCTNNNNNAGG 1 cut(s) 257
EcoT22I ATGCAT 1 cut(s) 291
EcoT38I GRGCYC 2 cut(s) 50, 178
FaeI CATG 3 cut(s) 289, 372, 489
FaiI YATR 5 cut(s) 117, 287, 362, 370, 487
FaqI GGGAC 2 cut(s) 81, 209
FatI CATG 3 cut(s) 285, 368, 485
FbaI TGATCA 1 cut(s) 280
Fnu4HI GCNGC 5 cut(s) 32, 35, 306, 339, 419
FokI GGATG 1 cut(s) 347
FriOI GRGCYC 2 cut(s) 50, 178
Fsp4HI GCNGC 5 cut(s) 32, 35, 306, 339, 419
FspBI CTAG 3 cut(s) 267, 410, 452
GluI GCNGC 5 cut(s) 32, 35, 306, 339, 419
GsaI CCCAGC 1 cut(s) 151
HapII CCGG 2 cut(s) 309, 459
HgaI GACGC 1 cut(s) 311
Hin1I GRCGYC 1 cut(s) 303
Hin1II CATG 3 cut(s) 289, 372, 489
HincII GTYRAC 1 cut(s) 466
HindII GTYRAC 1 cut(s) 466
HinfI GANTC 6 cut(s) 101, 137, 197, 323, 352, 455
HpaI GTTAAC 1 cut(s) 466
HpaII CCGG 2 cut(s) 309, 459
HphI GGTGA 1 cut(s) 214
Hpy166II GTNNAC 2 cut(s) 90, 466
Hpy188I TCNGA 6 cut(s) 52, 67, 136, 173, 259, 375
Hpy188III TCNNGA 7 cut(s) 98, 122, 179, 320, 430, 452, 459
Hpy8I GTNNAC 2 cut(s) 90, 466
Hpy99I CGWCG 2 cut(s) 131, 305
HpyAV CCTTC 2 cut(s) 388, 485
HpyCH4III ACNGT 1 cut(s) 87
HpyCH4IV ACGT 1 cut(s) 92
HpyCH4V TGCA 2 cut(s) 289, 418
HpyF10VI GCNNNNNNNGC 1 cut(s) 40
HpyF3I CTNAG 3 cut(s) 120, 345, 374
HpySE526I ACGT 1 cut(s) 92
Hsp92I GRCGYC 1 cut(s) 303
Hsp92II CATG 3 cut(s) 289, 372, 489
Kpn2I TCCGGA 1 cut(s) 458
Ksp22I TGATCA 1 cut(s) 280
KspAI GTTAAC 1 cut(s) 466
Kzo9I GATC 4 cut(s) 19, 79, 163, 280
LmnI GCTCC 4 cut(s) 31, 53, 137, 477
LpnPI CCDG 8 cut(s) 107, 161, 170, 317, 322, 428, 454, 472
Lsp1109I GCAGC 3 cut(s) 18, 325, 430
LweI GCATC 2 cut(s) 325, 436
MaeI CTAG 3 cut(s) 267, 410, 452
MaeII ACGT 1 cut(s) 92
MaeIII GTNAC 1 cut(s) 262
MalI GATC 4 cut(s) 21, 81, 165, 282
MboI GATC 4 cut(s) 19, 79, 163, 280
MboII GAAGA 4 cut(s) 20, 223, 398, 447
MhlI GDGCHC 2 cut(s) 50, 178
MluCI AATT 1 cut(s) 364
MlyI GAGTC 3 cut(s) 131, 191, 449
MmeI TCCRAC 2 cut(s) 90, 159
Mph1103I ATGCAT 1 cut(s) 291
MroI TCCGGA 1 cut(s) 458
MseI TTAA 2 cut(s) 380, 465
MspA1I CMGCKG 2 cut(s) 31, 151
MspI CCGG 2 cut(s) 309, 459
Mva1269I GAATGC 1 cut(s) 37
MvnI CGCG 1 cut(s) 212
MwoI GCNNNNNNNGC 1 cut(s) 40
NdeII GATC 4 cut(s) 19, 79, 163, 280
NlaIII CATG 3 cut(s) 289, 372, 489
NlaIV GGNNCC 2 cut(s) 133, 473
NmeAIII GCCGAG 1 cut(s) 69
NsiI ATGCAT 1 cut(s) 291
NspI RCATGY 1 cut(s) 289
PcsI WCGNNNNNNNCGW 2 cut(s) 132, 255
PctI GAATGC 1 cut(s) 37
PfeI GAWTC 3 cut(s) 101, 323, 352
PkrI GCNGC 5 cut(s) 33, 36, 307, 340, 420
PleI GAGTC 3 cut(s) 131, 191, 449
PmaCI CACGTG 1 cut(s) 93
PmlI CACGTG 1 cut(s) 93
PpsI GAGTC 3 cut(s) 131, 191, 449
Ppu21I YACGTR 1 cut(s) 93
Psp124BI GAGCTC 2 cut(s) 50, 178
PspCI CACGTG 1 cut(s) 93
PspFI CCCAGC 1 cut(s) 147
PspN4I GGNNCC 2 cut(s) 133, 473
PstI CTGCAG 1 cut(s) 420
PvuII CAGCTG 1 cut(s) 151
RsaI GTAC 1 cut(s) 359
RsaNI GTAC 1 cut(s) 358
SacI GAGCTC 2 cut(s) 50, 178
SaqAI TTAA 2 cut(s) 380, 465
SatI GCNGC 5 cut(s) 32, 35, 306, 339, 419
Sau3AI GATC 4 cut(s) 19, 79, 163, 280
SchI GAGTC 3 cut(s) 131, 191, 449
SduI GDGCHC 2 cut(s) 50, 178
SfaNI GCATC 2 cut(s) 325, 436
SfcI CTRYAG 1 cut(s) 416
SmlI CTYRAG 1 cut(s) 430
SmoI CTYRAG 1 cut(s) 430
Sse9I AATT 1 cut(s) 364
SsiI CCGC 5 cut(s) 29, 35, 212, 244, 306
SspMI CTAG 3 cut(s) 267, 410, 452
SstI GAGCTC 2 cut(s) 50, 178
TaaI ACNGT 1 cut(s) 87
TaiI ACGT 1 cut(s) 95
TaqI TCGA 2 cut(s) 78, 180
TaqII GACCGA 1 cut(s) 176
TasI AATT 1 cut(s) 364
TauI GCSGC 2 cut(s) 37, 308
TfiI GAWTC 3 cut(s) 101, 323, 352
Tru1I TTAA 2 cut(s) 380, 465
Tru9I TTAA 2 cut(s) 380, 465
TseI GCWGC 3 cut(s) 31, 338, 418
TspDTI ATGAA 3 cut(s) 357, 399, 407
TspGWI ACGGA 2 cut(s) 27, 316
XagI CCTNNNNNAGG 1 cut(s) 257
XapI RAATTY 1 cut(s) 364
XbaI TCTAGA 1 cut(s) 451
XceI RCATGY 1 cut(s) 289
XspI CTAG 3 cut(s) 267, 410, 452
Zsp2I ATGCAT 1 cut(s) 291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.