Rroxscaffold_3G00258360

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0006371
Physical Location & Seq
Forward (+)
52684642 .. 52689609
4968 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00258360.1

Sequence Viewer

Length: 477 bp
ATGGAGGAAGACGGAGGAGATCAAGCTCCGCTGCCGCATCCGCCGAGCTCCGAAACCCCAAACGTCGATCCACCTTCCACGTGTCACGATTCCACAGTCGCTCATACTGAGGACGACGGCTCCGACTCAAAACCCCAGCTGAACCAGACCGATCTATTCAGAGCTCTCGAAGTCGTTGAGAGGGACTCCCTCGCCATCGCGGAGAGCTTCACCTCTCTCTTTTCCTCCCTCCGCCTCGCCCTCTCGGAGGTTACTAGCAACTCGGTTGATCACATGCATTGCTTTGGCGACGCCGCTGGCCTGCTTCAAGAATCCGTGCTTGATGCTGCTACGAAGGGGAATCGGTACATAAATTCATGTCTGAGATTAAATGAAGAAATGAAGGGTGTTGATAGTCTAGCCCTGCAGCTAAAACTCTTGAGAAGAAATGTTGATGCTCTAGACTCCGGAGTTAACAGGCTCCTTCGCCTTCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BORCS6

Protein Analysis

158

Amino Acids

17.16

Weight (kDa)

4.58

Isoelectric Point (pI)

55.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BORCS6 PF10157 54 - 148 4.2e-20 BLOC-1-related complex sub-unit 6 C-terminal helix
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 200
AccIII TCCGGA 1 cut(s) 446
AciI CCGC 6 cut(s) 29, 35, 41, 200, 232, 294
AclWI GGATC 1 cut(s) 62
AcsI RAATTY 1 cut(s) 352
AcvI CACGTG 1 cut(s) 81
AcyI GRCGYC 1 cut(s) 291
AfaI GTAC 1 cut(s) 347
AfiI CCNNNNNNNGG 1 cut(s) 247
AflIII ACRYGT 1 cut(s) 80
AgsI TTSAA 1 cut(s) 308
AluBI AGCT 6 cut(s) 26, 48, 139, 164, 207, 409
AluI AGCT 6 cut(s) 26, 48, 139, 164, 207, 409
Alw21I GWGCWC 2 cut(s) 50, 166
AlwI GGATC 1 cut(s) 62
Aor13HI TCCGGA 1 cut(s) 446
AoxI GGCC 1 cut(s) 298
ApeKI GCWGC 3 cut(s) 31, 326, 406
ApoI RAATTY 1 cut(s) 352
AsuHPI GGTGA 1 cut(s) 202
BanII GRGCYC 2 cut(s) 50, 166
BbrPI CACGTG 1 cut(s) 81
BbsI GAAGAC 1 cut(s) 15
Bbv12I GWGCWC 2 cut(s) 50, 166
BbvI GCAGC 3 cut(s) 18, 313, 418
BccI CCATC 1 cut(s) 203
BceAI ACGGC 1 cut(s) 133
BclI TGATCA 1 cut(s) 268
BfaI CTAG 3 cut(s) 255, 398, 440
BfmI CTRYAG 1 cut(s) 404
BisI GCNGC 5 cut(s) 32, 35, 294, 327, 407
BlsI GCNGC 5 cut(s) 33, 36, 295, 328, 408
BmiI GGNNCC 2 cut(s) 121, 461
BmsI GCATC 3 cut(s) 46, 313, 424
BpiI GAAGAC 1 cut(s) 15
BplI GAGNNNNNCTC 2 cut(s) 170, 202
BpuEI CTTGAG 1 cut(s) 439
BsaAI YACGTR 1 cut(s) 81
BsaHI GRCGYC 1 cut(s) 291
BsaWI WCCGGW 1 cut(s) 446
BsaXI ACNNNNNCTCC 2 cut(s) 104, 134
Bsc4I CCNNNNNNNGG 1 cut(s) 247
Bse3DI GCAATG 1 cut(s) 277
BseAI TCCGGA 1 cut(s) 446
BseGI GGATG 1 cut(s) 37
BseLI CCNNNNNNNGG 1 cut(s) 247
BseMI GCAATG 1 cut(s) 277
BseMII CTCAG 2 cut(s) 99, 353
BseRI GAGGAG 1 cut(s) 30
BseXI GCAGC 3 cut(s) 18, 313, 418
BseYI CCCAGC 1 cut(s) 135
Bsh1236I CGCG 1 cut(s) 200
BshFI GGCC 1 cut(s) 300
BsiHKAI GWGCWC 2 cut(s) 50, 166
BsiSI CCGG 1 cut(s) 447
BslFI GGGAC 1 cut(s) 197
BslI CCNNNNNNNGG 1 cut(s) 247
BsmFI GGGAC 1 cut(s) 197
BsnI GGCC 1 cut(s) 300
Bsp1286I GDGCHC 2 cut(s) 50, 166
Bsp13I TCCGGA 1 cut(s) 446
Bsp143I GATC 4 cut(s) 19, 67, 151, 268
BspACI CCGC 6 cut(s) 29, 35, 41, 200, 232, 294
BspANI GGCC 1 cut(s) 300
BspCNI CTCAG 2 cut(s) 100, 354
BspEI TCCGGA 1 cut(s) 446
BspFNI CGCG 1 cut(s) 200
BspLI GGNNCC 2 cut(s) 121, 461
BspMAI CTGCAG 1 cut(s) 408
BspPI GGATC 1 cut(s) 62
BsrDI GCAATG 1 cut(s) 277
BssMI GATC 4 cut(s) 19, 67, 151, 268
BssNI GRCGYC 1 cut(s) 291
Bst4CI ACNGT 1 cut(s) 97
BstACI GRCGYC 1 cut(s) 291
BstBAI YACGTR 1 cut(s) 81
BstC8I GCNNGC 2 cut(s) 298, 302
BstDEI CTNAG 2 cut(s) 108, 362
BstENI CCTNNNNNAGG 1 cut(s) 245
BstF5I GGATG 1 cut(s) 37
BstFNI CGCG 1 cut(s) 200
BstKTI GATC 4 cut(s) 22, 70, 154, 271
BstMBI GATC 4 cut(s) 19, 67, 151, 268
BstMWI GCNNNNNNNGC 1 cut(s) 40
BstNSI RCATGY 1 cut(s) 277
BstSFI CTRYAG 1 cut(s) 404
BstUI CGCG 1 cut(s) 200
BstV1I GCAGC 3 cut(s) 18, 313, 418
BstV2I GAAGAC 1 cut(s) 15
BsuRI GGCC 1 cut(s) 300
BtgZI GCGATG 1 cut(s) 181
BtsCI GGATG 1 cut(s) 37
Cac8I GCNNGC 2 cut(s) 298, 302
CseI GACGC 1 cut(s) 299
Csp6I GTAC 1 cut(s) 346
CviAII CATG 3 cut(s) 274, 357, 474
CviQI GTAC 1 cut(s) 346
DdeI CTNAG 2 cut(s) 108, 362
DpnI GATC 4 cut(s) 21, 69, 153, 270
DpnII GATC 4 cut(s) 19, 67, 151, 268
EciI GGCGGA 2 cut(s) 30, 221
Ecl136II GAGCTC 2 cut(s) 48, 164
Eco24I GRGCYC 2 cut(s) 50, 166
Eco53kI GAGCTC 2 cut(s) 48, 164
Eco72I CACGTG 1 cut(s) 81
EcoICRI GAGCTC 2 cut(s) 48, 164
EcoNI CCTNNNNNAGG 1 cut(s) 245
EcoT22I ATGCAT 1 cut(s) 279
EcoT38I GRGCYC 2 cut(s) 50, 166
FaeI CATG 3 cut(s) 277, 360, 477
FaiI YATR 5 cut(s) 105, 275, 350, 358, 475
FaqI GGGAC 1 cut(s) 197
FatI CATG 3 cut(s) 273, 356, 473
FbaI TGATCA 1 cut(s) 268
Fnu4HI GCNGC 5 cut(s) 32, 35, 294, 327, 407
FokI GGATG 1 cut(s) 24
FriOI GRGCYC 2 cut(s) 50, 166
Fsp4HI GCNGC 5 cut(s) 32, 35, 294, 327, 407
FspBI CTAG 3 cut(s) 255, 398, 440
GluI GCNGC 5 cut(s) 32, 35, 294, 327, 407
GsaI CCCAGC 1 cut(s) 139
HaeIII GGCC 1 cut(s) 300
HapII CCGG 1 cut(s) 447
HgaI GACGC 1 cut(s) 299
Hin1I GRCGYC 1 cut(s) 291
Hin1II CATG 3 cut(s) 277, 360, 477
HincII GTYRAC 1 cut(s) 454
HindII GTYRAC 1 cut(s) 454
HinfI GANTC 6 cut(s) 89, 125, 185, 311, 340, 443
HpaI GTTAAC 1 cut(s) 454
HpaII CCGG 1 cut(s) 447
HphI GGTGA 1 cut(s) 202
Hpy166II GTNNAC 1 cut(s) 454
Hpy188I TCNGA 5 cut(s) 52, 124, 161, 247, 363
Hpy188III TCNNGA 6 cut(s) 86, 167, 308, 418, 440, 447
Hpy8I GTNNAC 1 cut(s) 454
Hpy99I CGWCG 3 cut(s) 68, 119, 293
HpyAV CCTTC 4 cut(s) 84, 328, 376, 473
HpyCH4III ACNGT 1 cut(s) 97
HpyCH4IV ACGT 2 cut(s) 63, 80
HpyCH4V TGCA 2 cut(s) 277, 406
HpyF10VI GCNNNNNNNGC 1 cut(s) 40
HpyF3I CTNAG 2 cut(s) 108, 362
HpySE526I ACGT 2 cut(s) 63, 80
Hsp92I GRCGYC 1 cut(s) 291
Hsp92II CATG 3 cut(s) 277, 360, 477
Kpn2I TCCGGA 1 cut(s) 446
Ksp22I TGATCA 1 cut(s) 268
KspAI GTTAAC 1 cut(s) 454
Kzo9I GATC 4 cut(s) 19, 67, 151, 268
LmnI GCTCC 4 cut(s) 31, 53, 125, 465
LpnPI CCDG 7 cut(s) 149, 158, 282, 314, 416, 442, 460
Lsp1109I GCAGC 3 cut(s) 18, 313, 418
LweI GCATC 3 cut(s) 46, 313, 424
MaeI CTAG 3 cut(s) 255, 398, 440
MaeII ACGT 2 cut(s) 63, 80
MaeIII GTNAC 2 cut(s) 83, 250
MalI GATC 4 cut(s) 21, 69, 153, 270
MboI GATC 4 cut(s) 19, 67, 151, 268
MboII GAAGA 3 cut(s) 20, 386, 435
MhlI GDGCHC 2 cut(s) 50, 166
MluCI AATT 1 cut(s) 352
MlyI GAGTC 3 cut(s) 119, 179, 437
MmeI TCCRAC 1 cut(s) 147
Mph1103I ATGCAT 1 cut(s) 279
MroI TCCGGA 1 cut(s) 446
MseI TTAA 2 cut(s) 368, 453
MspA1I CMGCKG 3 cut(s) 31, 139, 296
MspI CCGG 1 cut(s) 447
MvnI CGCG 1 cut(s) 200
MwoI GCNNNNNNNGC 1 cut(s) 40
NdeII GATC 4 cut(s) 19, 67, 151, 268
NlaIII CATG 3 cut(s) 277, 360, 477
NlaIV GGNNCC 2 cut(s) 121, 461
NmeAIII GCCGAG 1 cut(s) 69
NmuCI GTSAC 1 cut(s) 83
NsiI ATGCAT 1 cut(s) 279
NspI RCATGY 1 cut(s) 277
PcsI WCGNNNNNNNCGW 1 cut(s) 120
PfeI GAWTC 3 cut(s) 89, 311, 340
PkrI GCNGC 5 cut(s) 33, 36, 295, 328, 408
PleI GAGTC 3 cut(s) 119, 179, 437
PmaCI CACGTG 1 cut(s) 81
PmlI CACGTG 1 cut(s) 81
PpsI GAGTC 3 cut(s) 119, 179, 437
Ppu21I YACGTR 1 cut(s) 81
Psp124BI GAGCTC 2 cut(s) 50, 166
PspCI CACGTG 1 cut(s) 81
PspFI CCCAGC 1 cut(s) 135
PspN4I GGNNCC 2 cut(s) 121, 461
PstI CTGCAG 1 cut(s) 408
PvuII CAGCTG 1 cut(s) 139
RsaI GTAC 1 cut(s) 347
RsaNI GTAC 1 cut(s) 346
SacI GAGCTC 2 cut(s) 50, 166
SaqAI TTAA 2 cut(s) 368, 453
SatI GCNGC 5 cut(s) 32, 35, 294, 327, 407
Sau3AI GATC 4 cut(s) 19, 67, 151, 268
SchI GAGTC 3 cut(s) 119, 179, 437
SduI GDGCHC 2 cut(s) 50, 166
SfaNI GCATC 3 cut(s) 46, 313, 424
SfcI CTRYAG 1 cut(s) 404
SmlI CTYRAG 1 cut(s) 418
SmoI CTYRAG 1 cut(s) 418
Sse9I AATT 1 cut(s) 352
SsiI CCGC 6 cut(s) 29, 35, 41, 200, 232, 294
SspMI CTAG 3 cut(s) 255, 398, 440
SstI GAGCTC 2 cut(s) 50, 166
TaaI ACNGT 1 cut(s) 97
TaiI ACGT 2 cut(s) 66, 83
TaqI TCGA 2 cut(s) 66, 168
TaqII GACCGA 1 cut(s) 164
TasI AATT 1 cut(s) 352
TauI GCSGC 2 cut(s) 37, 296
TfiI GAWTC 3 cut(s) 89, 311, 340
Tru1I TTAA 2 cut(s) 368, 453
Tru9I TTAA 2 cut(s) 368, 453
TseFI GTSAC 1 cut(s) 83
TseI GCWGC 3 cut(s) 31, 326, 406
Tsp45I GTSAC 1 cut(s) 83
TspDTI ATGAA 3 cut(s) 345, 387, 395
TspGWI ACGGA 2 cut(s) 27, 304
XagI CCTNNNNNAGG 1 cut(s) 245
XapI RAATTY 1 cut(s) 352
XbaI TCTAGA 1 cut(s) 439
XceI RCATGY 1 cut(s) 277
XspI CTAG 3 cut(s) 255, 398, 440
Zsp2I ATGCAT 1 cut(s) 279
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.