Rmu_sc0024523.1_g000002

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0024523.1
OG0006371
Physical Location & Seq
Forward (+)
1975 .. 2557
583 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0024523.1_g000002.1.cds

Sequence Viewer

Length: 381 bp
atggaggaagacggaggagatcaagctccgctgccgcatccgccgagctccgaaaccccaatctccgacccaaacctcgatccaccgtccacgtgtcccgattccacagtcgctcatactgaggacgacggctccgactcaaaatctcagctgaaccagaccgatctattcagagctgtcgaagtcgtcgagagggactccctcgccatcgcggagagcttcaactctctcttctcctccctccgcctcgccctctccgaagttactagcaactcggttgatcacatgcattgctttggcgacgccgccggccggcttcaagaatccgtgcttgatgctgctacgaaggggaatcgctacataaattcatgtctgaggtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BORCS6

Protein Analysis

126

Amino Acids

13.53

Weight (kDa)

4.3

Isoelectric Point (pI)

53.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 212
AciI CCGC 6 cut(s) 29, 35, 41, 212, 244, 306
AclWI GGATC 1 cut(s) 74
AcoI YGGCCR 1 cut(s) 310
AcsI RAATTY 1 cut(s) 364
AcvI CACGTG 1 cut(s) 93
AcyI GRCGYC 1 cut(s) 303
AfiI CCNNNNNNNGG 1 cut(s) 312
AflIII ACRYGT 1 cut(s) 92
AgsI TTSAA 2 cut(s) 223, 320
AluBI AGCT 5 cut(s) 26, 48, 151, 176, 219
AluI AGCT 5 cut(s) 26, 48, 151, 176, 219
Alw21I GWGCWC 1 cut(s) 50
AlwI GGATC 1 cut(s) 74
AoxI GGCC 1 cut(s) 310
ApeKI GCWGC 2 cut(s) 31, 338
ApoI RAATTY 1 cut(s) 364
BanII GRGCYC 1 cut(s) 50
BbrPI CACGTG 1 cut(s) 93
BbsI GAAGAC 1 cut(s) 15
Bbv12I GWGCWC 1 cut(s) 50
BbvI GCAGC 2 cut(s) 18, 325
BccI CCATC 1 cut(s) 215
BceAI ACGGC 1 cut(s) 145
BclI TGATCA 1 cut(s) 280
BfaI CTAG 1 cut(s) 267
BisI GCNGC 4 cut(s) 32, 35, 306, 339
BlsI GCNGC 4 cut(s) 33, 36, 307, 340
BmiI GGNNCC 1 cut(s) 133
BmsI GCATC 2 cut(s) 46, 325
BpiI GAAGAC 1 cut(s) 15
BplI GAGNNNNNCTC 2 cut(s) 182, 214
BsaAI YACGTR 1 cut(s) 93
BsaHI GRCGYC 1 cut(s) 303
BsaXI ACNNNNNCTCC 2 cut(s) 116, 146
Bsc4I CCNNNNNNNGG 1 cut(s) 312
Bse118I RCCGGY 2 cut(s) 308, 312
Bse3DI GCAATG 1 cut(s) 289
BseGI GGATG 1 cut(s) 37
BseLI CCNNNNNNNGG 1 cut(s) 312
BseMI GCAATG 1 cut(s) 289
BseMII CTCAG 3 cut(s) 111, 161, 365
BseRI GAGGAG 2 cut(s) 30, 226
BseX3I CGGCCG 1 cut(s) 310
BseXI GCAGC 2 cut(s) 18, 325
Bsh1236I CGCG 1 cut(s) 212
Bsh1285I CGRYCG 1 cut(s) 313
BshFI GGCC 1 cut(s) 312
BsiEI CGRYCG 1 cut(s) 313
BsiHKAI GWGCWC 1 cut(s) 50
BsiSI CCGG 2 cut(s) 309, 313
BslFI GGGAC 2 cut(s) 81, 209
BslI CCNNNNNNNGG 1 cut(s) 312
BsmFI GGGAC 2 cut(s) 81, 209
BsnI GGCC 1 cut(s) 312
Bsp1286I GDGCHC 1 cut(s) 50
Bsp143I GATC 4 cut(s) 19, 79, 163, 280
BspACI CCGC 6 cut(s) 29, 35, 41, 212, 244, 306
BspANI GGCC 1 cut(s) 312
BspCNI CTCAG 3 cut(s) 112, 160, 366
BspFNI CGCG 1 cut(s) 212
BspLI GGNNCC 1 cut(s) 133
BspPI GGATC 1 cut(s) 74
BsrDI GCAATG 1 cut(s) 289
BsrFI RCCGGY 2 cut(s) 308, 312
BssAI RCCGGY 2 cut(s) 308, 312
BssMI GATC 4 cut(s) 19, 79, 163, 280
BssNI GRCGYC 1 cut(s) 303
Bst4CI ACNGT 2 cut(s) 87, 109
Bst6I CTCTTC 1 cut(s) 236
BstACI GRCGYC 1 cut(s) 303
BstBAI YACGTR 1 cut(s) 93
BstC8I GCNNGC 2 cut(s) 310, 314
BstDEI CTNAG 3 cut(s) 120, 147, 374
BstF5I GGATG 1 cut(s) 37
BstFNI CGCG 1 cut(s) 212
BstKTI GATC 4 cut(s) 22, 82, 166, 283
BstMBI GATC 4 cut(s) 19, 79, 163, 280
BstMCI CGRYCG 1 cut(s) 313
BstMWI GCNNNNNNNGC 1 cut(s) 40
BstNSI RCATGY 1 cut(s) 289
BstUI CGCG 1 cut(s) 212
BstV1I GCAGC 2 cut(s) 18, 325
BstV2I GAAGAC 1 cut(s) 15
BstZI CGGCCG 1 cut(s) 310
BsuRI GGCC 1 cut(s) 312
BtgZI GCGATG 1 cut(s) 193
BtsCI GGATG 1 cut(s) 37
Cac8I GCNNGC 2 cut(s) 310, 314
Cfr10I RCCGGY 2 cut(s) 308, 312
CseI GACGC 1 cut(s) 311
CviAII CATG 2 cut(s) 286, 369
CviJI RGCY 8 cut(s) 26, 48, 132, 151, 176, 219, 312, 316
CviKI_1 RGCY 8 cut(s) 26, 48, 132, 151, 176, 219, 312, 316
DdeI CTNAG 3 cut(s) 120, 147, 374
DpnI GATC 4 cut(s) 21, 81, 165, 282
DpnII GATC 4 cut(s) 19, 79, 163, 280
EaeI YGGCCR 1 cut(s) 310
EagI CGGCCG 1 cut(s) 310
Eam1104I CTCTTC 1 cut(s) 236
EarI CTCTTC 1 cut(s) 236
EciI GGCGGA 2 cut(s) 30, 233
Ecl136II GAGCTC 1 cut(s) 48
EclXI CGGCCG 1 cut(s) 310
Eco24I GRGCYC 1 cut(s) 50
Eco52I CGGCCG 1 cut(s) 310
Eco53kI GAGCTC 1 cut(s) 48
Eco72I CACGTG 1 cut(s) 93
EcoICRI GAGCTC 1 cut(s) 48
EcoT22I ATGCAT 1 cut(s) 291
EcoT38I GRGCYC 1 cut(s) 50
FaeI CATG 2 cut(s) 289, 372
FaiI YATR 4 cut(s) 117, 287, 362, 370
FaqI GGGAC 2 cut(s) 81, 209
FatI CATG 2 cut(s) 285, 368
FbaI TGATCA 1 cut(s) 280
Fnu4HI GCNGC 4 cut(s) 32, 35, 306, 339
FokI GGATG 1 cut(s) 24
FriOI GRGCYC 1 cut(s) 50
Fsp4HI GCNGC 4 cut(s) 32, 35, 306, 339
FspBI CTAG 1 cut(s) 267
GluI GCNGC 4 cut(s) 32, 35, 306, 339
HaeIII GGCC 1 cut(s) 312
HapII CCGG 2 cut(s) 309, 313
HgaI GACGC 1 cut(s) 311
Hin1I GRCGYC 1 cut(s) 303
Hin1II CATG 2 cut(s) 289, 372
HinfI GANTC 5 cut(s) 101, 137, 197, 323, 352
HpaII CCGG 2 cut(s) 309, 313
Hpy166II GTNNAC 1 cut(s) 90
Hpy188I TCNGA 6 cut(s) 52, 67, 136, 173, 259, 375
Hpy188III TCNNGA 3 cut(s) 98, 190, 320
Hpy8I GTNNAC 1 cut(s) 90
Hpy99I CGWCG 3 cut(s) 131, 191, 305
HpyAV CCTTC 1 cut(s) 340
HpyCH4III ACNGT 2 cut(s) 87, 109
HpyCH4IV ACGT 1 cut(s) 92
HpyCH4V TGCA 1 cut(s) 289
HpyF10VI GCNNNNNNNGC 1 cut(s) 40
HpyF3I CTNAG 3 cut(s) 120, 147, 374
HpySE526I ACGT 1 cut(s) 92
Hsp92I GRCGYC 1 cut(s) 303
Hsp92II CATG 2 cut(s) 289, 372
KroI GCCGGC 2 cut(s) 308, 312
KroNI GCCGGC 2 cut(s) 310, 314
Ksp22I TGATCA 1 cut(s) 280
Kzo9I GATC 4 cut(s) 19, 79, 163, 280
LmnI GCTCC 3 cut(s) 31, 53, 137
LpnPI CCDG 3 cut(s) 170, 322, 326
Lsp1109I GCAGC 2 cut(s) 18, 325
LweI GCATC 2 cut(s) 46, 325
MaeI CTAG 1 cut(s) 267
MaeII ACGT 1 cut(s) 92
MaeIII GTNAC 1 cut(s) 262
MalI GATC 4 cut(s) 21, 81, 165, 282
MboI GATC 4 cut(s) 19, 79, 163, 280
MboII GAAGA 2 cut(s) 20, 223
MhlI GDGCHC 1 cut(s) 50
MluCI AATT 1 cut(s) 364
MlyI GAGTC 2 cut(s) 131, 191
MmeI TCCRAC 2 cut(s) 90, 159
Mph1103I ATGCAT 1 cut(s) 291
MroNI GCCGGC 2 cut(s) 308, 312
MspA1I CMGCKG 2 cut(s) 31, 151
MspI CCGG 2 cut(s) 309, 313
MvnI CGCG 1 cut(s) 212
MwoI GCNNNNNNNGC 1 cut(s) 40
NaeI GCCGGC 2 cut(s) 310, 314
NdeII GATC 4 cut(s) 19, 79, 163, 280
NgoMIV GCCGGC 2 cut(s) 308, 312
NlaIII CATG 2 cut(s) 289, 372
NlaIV GGNNCC 1 cut(s) 133
NmeAIII GCCGAG 1 cut(s) 69
NsiI ATGCAT 1 cut(s) 291
NspI RCATGY 1 cut(s) 289
PcsI WCGNNNNNNNCGW 3 cut(s) 132, 186, 255
PdiI GCCGGC 2 cut(s) 310, 314
PfeI GAWTC 3 cut(s) 101, 323, 352
PkrI GCNGC 4 cut(s) 33, 36, 307, 340
PleI GAGTC 2 cut(s) 131, 191
PmaCI CACGTG 1 cut(s) 93
PmlI CACGTG 1 cut(s) 93
PpsI GAGTC 2 cut(s) 131, 191
Ppu21I YACGTR 1 cut(s) 93
Psp124BI GAGCTC 1 cut(s) 50
PspCI CACGTG 1 cut(s) 93
PspN4I GGNNCC 1 cut(s) 133
PvuII CAGCTG 1 cut(s) 151
SacI GAGCTC 1 cut(s) 50
SatI GCNGC 4 cut(s) 32, 35, 306, 339
Sau3AI GATC 4 cut(s) 19, 79, 163, 280
SchI GAGTC 2 cut(s) 131, 191
SduI GDGCHC 1 cut(s) 50
SetI ASST 8 cut(s) 28, 50, 78, 95, 153, 178, 221, 380
SfaNI GCATC 2 cut(s) 46, 325
Sse9I AATT 1 cut(s) 364
SsiI CCGC 6 cut(s) 29, 35, 41, 212, 244, 306
SspMI CTAG 1 cut(s) 267
SstI GAGCTC 1 cut(s) 50
TaaI ACNGT 2 cut(s) 87, 109
TaiI ACGT 1 cut(s) 95
TaqI TCGA 3 cut(s) 78, 180, 189
TaqII GACCGA 1 cut(s) 176
TasI AATT 1 cut(s) 364
TauI GCSGC 2 cut(s) 37, 308
TfiI GAWTC 3 cut(s) 101, 323, 352
TseI GCWGC 2 cut(s) 31, 338
TspDTI ATGAA 1 cut(s) 357
TspGWI ACGGA 2 cut(s) 27, 316
XapI RAATTY 1 cut(s) 364
XceI RCATGY 1 cut(s) 289
XspI CTAG 1 cut(s) 267
Zsp2I ATGCAT 1 cut(s) 291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.