MD14G1105400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0006371
Physical Location & Seq
Forward (+)
16353762 .. 16355892
2131 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1105400.v1.1.491

Sequence Viewer

Length: 492 bp
ATGGAGGAAGACGGAGGAGAACCACCTCAACTGCCCCGTCCGACGAGCTCCAAAGCTCCAATCTCAGTGCCACGCCTCGATCCGCAGTACGCTTGTCCTGAATCCACAGTCGACCCCAGTGAGGACGATCGCACCGATTCGAAACCCACCTTGCTGAACCAGACCGAGTTATTCCGAGCTCTCGAAGTGGTCGAGAGAGACTCCCTGGCCATCGCCGATAGCTTCACGTCCCTCTTCGCCTCCCTCCGCCTGGCCCTCTCAGAGGTTACTAGCAACTCAGCTGATCACATGCATTGCTTTGGTGAAGCTGCTGGCCGCCTTCAAGAATCTGTTCTTGATGCTGCTACCAAGGGAAACCGGTATATAAACTCATGTCTCAGATTAAACGAAGAAATGAAGAGCGCTGGCAGTCTAGCCTTGCAGCTAAAAATCTTGAGGAGAAATGTTGACGCTCTCGACTCAGGGGTGAACAAGCTCCTTCGCCTTCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BORCS6

Protein Analysis

164

Amino Acids

17.81

Weight (kDa)

4.88

Isoelectric Point (pI)

60.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BORCS6 PF10157 59 - 153 2.7e-18 BLOC-1-related complex sub-unit 6 C-terminal helix
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 111
AciI CCGC 3 cut(s) 83, 247, 316
AclWI GGATC 1 cut(s) 74
AcoI YGGCCR 2 cut(s) 207, 313
AfaI GTAC 1 cut(s) 89
AfeI AGCGCT 1 cut(s) 403
AfiI CCNNNNNNNGG 3 cut(s) 121, 250, 262
AgeI ACCGGT 1 cut(s) 357
AgsI TTSAA 1 cut(s) 323
AjiI CACGTC 1 cut(s) 228
AjnI CCWGG 2 cut(s) 204, 249
AluBI AGCT 8 cut(s) 48, 56, 179, 222, 281, 308, 424, 475
AluI AGCT 8 cut(s) 48, 56, 179, 222, 281, 308, 424, 475
Alw21I GWGCWC 2 cut(s) 50, 181
Alw26I GTCTC 2 cut(s) 192, 380
AlwI GGATC 1 cut(s) 74
Aor51HI AGCGCT 1 cut(s) 403
AoxI GGCC 3 cut(s) 207, 252, 313
ApeKI GCWGC 3 cut(s) 308, 341, 421
AsiGI ACCGGT 1 cut(s) 357
Asp700I GAANNNNTTC 1 cut(s) 330
AspLEI GCGC 1 cut(s) 404
AspS9I GGNCC 1 cut(s) 253
AsuHPI GGTGA 2 cut(s) 314, 478
AsuII TTCGAA 1 cut(s) 140
BalI TGGCCA 1 cut(s) 209
BanII GRGCYC 2 cut(s) 50, 181
BbsI GAAGAC 1 cut(s) 15
Bbv12I GWGCWC 2 cut(s) 50, 181
BbvI GCAGC 3 cut(s) 295, 328, 433
BccI CCATC 1 cut(s) 218
BciT130I CCWGG 2 cut(s) 206, 251
BclI TGATCA 1 cut(s) 283
BcoDI GTCTC 2 cut(s) 192, 380
BfaI CTAG 2 cut(s) 270, 413
BfoI RGCGCY 1 cut(s) 405
BisI GCNGC 4 cut(s) 309, 316, 342, 422
BlsI GCNGC 4 cut(s) 310, 317, 343, 423
Bme1390I CCNGG 2 cut(s) 206, 251
BmgBI CACGTC 1 cut(s) 228
BmgT120I GGNCC 1 cut(s) 253
BmrFI CCNGG 2 cut(s) 206, 251
BmrI ACTGGG 1 cut(s) 111
BmsI GCATC 1 cut(s) 328
BmuI ACTGGG 1 cut(s) 111
BpiI GAAGAC 1 cut(s) 15
BplI GAGNNNNNCTC 2 cut(s) 185, 217
Bpu14I TTCGAA 1 cut(s) 140
BpuEI CTTGAG 1 cut(s) 454
BsaJI CCNNGG 2 cut(s) 204, 348
BsaWI WCCGGW 1 cut(s) 357
Bsc4I CCNNNNNNNGG 3 cut(s) 121, 250, 262
Bse118I RCCGGY 1 cut(s) 357
Bse1I ACTGG 1 cut(s) 117
Bse3DI GCAATG 1 cut(s) 292
BseBI CCWGG 2 cut(s) 206, 251
BseDI CCNNGG 2 cut(s) 204, 348
BseLI CCNNNNNNNGG 3 cut(s) 121, 250, 262
BseMI GCAATG 1 cut(s) 292
BseMII CTCAG 5 cut(s) 78, 273, 291, 391, 474
BseNI ACTGG 1 cut(s) 117
BseRI GAGGAG 2 cut(s) 30, 451
BseXI GCAGC 3 cut(s) 295, 328, 433
Bsh1285I CGRYCG 1 cut(s) 130
BshFI GGCC 3 cut(s) 209, 254, 315
BshTI ACCGGT 1 cut(s) 357
BsiEI CGRYCG 1 cut(s) 130
BsiHKAI GWGCWC 2 cut(s) 50, 181
BsiSI CCGG 1 cut(s) 358
BslFI GGGAC 1 cut(s) 214
BslI CCNNNNNNNGG 3 cut(s) 121, 250, 262
BsmAI GTCTC 2 cut(s) 192, 380
BsmFI GGGAC 1 cut(s) 214
BsnI GGCC 3 cut(s) 209, 254, 315
Bsp119I TTCGAA 1 cut(s) 140
Bsp1286I GDGCHC 2 cut(s) 50, 181
Bsp143I GATC 3 cut(s) 79, 127, 283
BspACI CCGC 3 cut(s) 83, 247, 316
BspANI GGCC 3 cut(s) 209, 254, 315
BspCNI CTCAG 5 cut(s) 77, 272, 290, 390, 473
BspPI GGATC 1 cut(s) 74
BspQI GCTCTTC 1 cut(s) 392
BspT104I TTCGAA 1 cut(s) 140
BsrDI GCAATG 1 cut(s) 292
BsrFI RCCGGY 1 cut(s) 357
BsrI ACTGG 1 cut(s) 117
BssAI RCCGGY 1 cut(s) 357
BssECI CCNNGG 2 cut(s) 204, 348
BssMI GATC 3 cut(s) 79, 127, 283
BssT1I CCWWGG 1 cut(s) 348
Bst2UI CCWGG 2 cut(s) 206, 251
Bst4CI ACNGT 1 cut(s) 109
Bst6I CTCTTC 2 cut(s) 239, 392
BstBI TTCGAA 1 cut(s) 140
BstC8I GCNNGC 2 cut(s) 313, 406
BstDEI CTNAG 5 cut(s) 64, 259, 277, 377, 460
BstENI CCTNNNNNAGG 1 cut(s) 260
BstH2I RGCGCY 1 cut(s) 405
BstHHI GCGC 1 cut(s) 404
BstKTI GATC 3 cut(s) 82, 130, 286
BstMAI GTCTC 2 cut(s) 192, 380
BstMBI GATC 3 cut(s) 79, 127, 283
BstMCI CGRYCG 1 cut(s) 130
BstNI CCWGG 2 cut(s) 206, 251
BstNSI RCATGY 1 cut(s) 292
BstSCI CCNGG 2 cut(s) 204, 249
BstV1I GCAGC 3 cut(s) 295, 328, 433
BstV2I GAAGAC 1 cut(s) 15
BsuRI GGCC 3 cut(s) 209, 254, 315
BtgZI GCGATG 1 cut(s) 196
BtrI CACGTC 1 cut(s) 228
BtsIMutI CAGTG 2 cut(s) 72, 124
Cac8I GCNNGC 2 cut(s) 313, 406
CfoI GCGC 1 cut(s) 404
Cfr10I RCCGGY 1 cut(s) 357
Cfr13I GGNCC 1 cut(s) 253
CseI GACGC 1 cut(s) 458
Csp6I GTAC 1 cut(s) 88
CspAI ACCGGT 1 cut(s) 357
CviAII CATG 3 cut(s) 289, 372, 489
CviQI GTAC 1 cut(s) 88
DdeI CTNAG 5 cut(s) 64, 259, 277, 377, 460
DpnI GATC 3 cut(s) 81, 129, 285
DpnII GATC 3 cut(s) 79, 127, 283
EaeI YGGCCR 2 cut(s) 207, 313
Eam1104I CTCTTC 2 cut(s) 239, 392
EarI CTCTTC 2 cut(s) 239, 392
EciI GGCGGA 1 cut(s) 236
Ecl136II GAGCTC 2 cut(s) 48, 179
Eco130I CCWWGG 1 cut(s) 348
Eco24I GRGCYC 2 cut(s) 50, 181
Eco47III AGCGCT 1 cut(s) 403
Eco53kI GAGCTC 2 cut(s) 48, 179
EcoICRI GAGCTC 2 cut(s) 48, 179
EcoNI CCTNNNNNAGG 1 cut(s) 260
EcoRII CCWGG 2 cut(s) 204, 249
EcoT14I CCWWGG 1 cut(s) 348
EcoT22I ATGCAT 1 cut(s) 294
EcoT38I GRGCYC 2 cut(s) 50, 181
ErhI CCWWGG 1 cut(s) 348
FaeI CATG 3 cut(s) 292, 375, 492
FaiI YATR 5 cut(s) 290, 363, 365, 373, 490
FaqI GGGAC 1 cut(s) 214
FatI CATG 3 cut(s) 288, 371, 488
FbaI TGATCA 1 cut(s) 283
FblI GTMKAC 1 cut(s) 111
Fnu4HI GCNGC 4 cut(s) 309, 316, 342, 422
FriOI GRGCYC 2 cut(s) 50, 181
Fsp4HI GCNGC 4 cut(s) 309, 316, 342, 422
FspBI CTAG 2 cut(s) 270, 413
GlaI GCGC 1 cut(s) 403
GluI GCNGC 4 cut(s) 309, 316, 342, 422
HaeII RGCGCY 1 cut(s) 405
HaeIII GGCC 3 cut(s) 209, 254, 315
HapII CCGG 1 cut(s) 358
HgaI GACGC 1 cut(s) 458
HhaI GCGC 1 cut(s) 404
Hin1II CATG 3 cut(s) 292, 375, 492
Hin6I GCGC 1 cut(s) 402
HinP1I GCGC 1 cut(s) 402
HincII GTYRAC 2 cut(s) 112, 448
HindII GTYRAC 2 cut(s) 112, 448
HinfI GANTC 5 cut(s) 101, 137, 200, 326, 458
HpaII CCGG 1 cut(s) 358
HphI GGTGA 2 cut(s) 314, 478
Hpy166II GTNNAC 3 cut(s) 112, 448, 469
Hpy188I TCNGA 4 cut(s) 42, 176, 262, 380
Hpy188III TCNNGA 7 cut(s) 98, 182, 193, 323, 335, 433, 455
Hpy8I GTNNAC 3 cut(s) 112, 448, 469
Hpy99I CGWCG 1 cut(s) 46
HpyAV CCTTC 2 cut(s) 329, 488
HpyCH4III ACNGT 1 cut(s) 109
HpyCH4IV ACGT 1 cut(s) 227
HpyCH4V TGCA 2 cut(s) 292, 421
HpyF3I CTNAG 5 cut(s) 64, 259, 277, 377, 460
HpySE526I ACGT 1 cut(s) 227
Hsp92II CATG 3 cut(s) 292, 375, 492
HspAI GCGC 1 cut(s) 402
Ksp22I TGATCA 1 cut(s) 283
Kzo9I GATC 3 cut(s) 79, 127, 283
LguI GCTCTTC 1 cut(s) 392
LmnI GCTCC 3 cut(s) 53, 61, 480
Lsp1109I GCAGC 3 cut(s) 295, 328, 433
LweI GCATC 1 cut(s) 328
MaeI CTAG 2 cut(s) 270, 413
MaeII ACGT 1 cut(s) 227
MaeIII GTNAC 1 cut(s) 265
MalI GATC 3 cut(s) 81, 129, 285
MboI GATC 3 cut(s) 79, 127, 283
MboII GAAGA 4 cut(s) 20, 226, 401, 409
MhlI GDGCHC 2 cut(s) 50, 181
MlsI TGGCCA 1 cut(s) 209
MluNI TGGCCA 1 cut(s) 209
MlyI GAGTC 2 cut(s) 194, 452
MmeI TCCRAC 1 cut(s) 65
Mox20I TGGCCA 1 cut(s) 209
Mph1103I ATGCAT 1 cut(s) 294
MroXI GAANNNNTTC 1 cut(s) 330
MscI TGGCCA 1 cut(s) 209
MseI TTAA 1 cut(s) 383
Msp20I TGGCCA 1 cut(s) 209
MspA1I CMGCKG 1 cut(s) 281
MspI CCGG 1 cut(s) 358
MspR9I CCNGG 2 cut(s) 206, 251
MvaI CCWGG 2 cut(s) 206, 251
NdeII GATC 3 cut(s) 79, 127, 283
NlaIII CATG 3 cut(s) 292, 375, 492
NsiI ATGCAT 1 cut(s) 294
NspI RCATGY 1 cut(s) 292
NspV TTCGAA 1 cut(s) 140
PciSI GCTCTTC 1 cut(s) 392
PcsI WCGNNNNNNNCGW 2 cut(s) 132, 189
PdmI GAANNNNTTC 1 cut(s) 330
PfeI GAWTC 3 cut(s) 101, 137, 326
PinAI ACCGGT 1 cut(s) 357
PkrI GCNGC 4 cut(s) 310, 317, 343, 423
Ple19I CGATCG 1 cut(s) 130
PleI GAGTC 2 cut(s) 194, 452
PpsI GAGTC 2 cut(s) 194, 452
Psp124BI GAGCTC 2 cut(s) 50, 181
Psp6I CCWGG 2 cut(s) 204, 249
PspGI CCWGG 2 cut(s) 204, 249
PspPI GGNCC 1 cut(s) 253
PvuI CGATCG 1 cut(s) 130
PvuII CAGCTG 1 cut(s) 281
RsaI GTAC 1 cut(s) 89
RsaNI GTAC 1 cut(s) 88
SacI GAGCTC 2 cut(s) 50, 181
SalI GTCGAC 1 cut(s) 110
SapI GCTCTTC 1 cut(s) 392
SaqAI TTAA 1 cut(s) 383
SatI GCNGC 4 cut(s) 309, 316, 342, 422
Sau3AI GATC 3 cut(s) 79, 127, 283
Sau96I GGNCC 1 cut(s) 253
SchI GAGTC 2 cut(s) 194, 452
ScrFI CCNGG 2 cut(s) 206, 251
SduI GDGCHC 2 cut(s) 50, 181
SfaNI GCATC 1 cut(s) 328
SfuI TTCGAA 1 cut(s) 140
SmlI CTYRAG 1 cut(s) 433
SmoI CTYRAG 1 cut(s) 433
SsiI CCGC 3 cut(s) 83, 247, 316
SspMI CTAG 2 cut(s) 270, 413
SstI GAGCTC 2 cut(s) 50, 181
StyD4I CCNGG 2 cut(s) 204, 249
StyI CCWWGG 1 cut(s) 348
TaaI ACNGT 1 cut(s) 109
TaiI ACGT 1 cut(s) 230
TaqI TCGA 6 cut(s) 78, 111, 140, 183, 192, 456
TaqII GACCGA 1 cut(s) 179
TauI GCSGC 1 cut(s) 318
TfiI GAWTC 3 cut(s) 101, 137, 326
Tru1I TTAA 1 cut(s) 383
Tru9I TTAA 1 cut(s) 383
TscAI CASTG 2 cut(s) 72, 124
TseI GCWGC 3 cut(s) 308, 341, 421
TspDTI ATGAA 1 cut(s) 410
TspGWI ACGGA 1 cut(s) 27
TspRI CASTG 2 cut(s) 72, 124
XagI CCTNNNNNAGG 1 cut(s) 260
XceI RCATGY 1 cut(s) 292
XmiI GTMKAC 1 cut(s) 111
XmnI GAANNNNTTC 1 cut(s) 330
XspI CTAG 2 cut(s) 270, 413
Zsp2I ATGCAT 1 cut(s) 294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.