MD06G1084200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0006371
Physical Location & Seq
Forward (+)
20567679 .. 20582893
15215 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1084200.v1.1.491

Sequence Viewer

Length: 492 bp
ATGGAGGAAGACGGAGGAGAACCACCTCGACCGCCCAATCCGACGAGCTCCGAAGCCCTAACCTCGGACCCACGTCTCGGTCCACAGTCCACGTGTCCTGATTCCACACTCGATCCCAGTGAGGACAACCGCACCGATTCGAAACCCCCTCCGATGAACCAGACCGAGATTTTCCGAGCTCTCGAAGTGGTCGAGAGAGACTCCCTGGCCATCGCCGACAGCTTCACCTCCCTCTTCGCCTCCCTCCGCCTGGCCCTCTCCGAGGTTACTAGCAACTCAGTTGATCACATGCATTGCTTTGGTGACGCTGCTGGCCGCCTTCAAGAATCAGTGCTTGATGCTGCTACCAAGGGAAATCGGTATATAAATTCATGTCTCAGATTAAATGAAGAAATGAAGGGCATTGACAATCTAGCCTTGCAGCTAAAAATCTTGAGGAGAAATGTTGATGCTCTCGACTCAGGTGTGAACAAGCTCCTTCGTCTTCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BORCS6

Protein Analysis

164

Amino Acids

17.81

Weight (kDa)

4.69

Isoelectric Point (pI)

58.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BORCS6 PF10157 59 - 153 1.8e-20 BLOC-1-related complex sub-unit 6 C-terminal helix
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 32, 130, 247, 316
AclWI GGATC 1 cut(s) 107
AcoI YGGCCR 2 cut(s) 207, 313
AcsI RAATTY 1 cut(s) 367
AcvI CACGTG 1 cut(s) 93
AfiI CCNNNNNNNGG 4 cut(s) 64, 77, 250, 262
AflIII ACRYGT 1 cut(s) 92
AgsI TTSAA 1 cut(s) 323
AjiI CACGTC 1 cut(s) 74
AjnI CCWGG 2 cut(s) 204, 249
AluBI AGCT 5 cut(s) 48, 179, 222, 424, 475
AluI AGCT 5 cut(s) 48, 179, 222, 424, 475
Alw21I GWGCWC 2 cut(s) 50, 181
Alw26I GTCTC 3 cut(s) 80, 192, 380
AlwI GGATC 1 cut(s) 107
AoxI GGCC 3 cut(s) 207, 252, 313
ApeKI GCWGC 3 cut(s) 308, 341, 421
ApoI RAATTY 1 cut(s) 367
AspS9I GGNCC 3 cut(s) 67, 80, 253
AsuHPI GGTGA 2 cut(s) 217, 314
AsuII TTCGAA 1 cut(s) 140
AvaII GGWCC 2 cut(s) 67, 80
BalI TGGCCA 1 cut(s) 209
BanII GRGCYC 2 cut(s) 50, 181
BbrPI CACGTG 1 cut(s) 93
BbsI GAAGAC 2 cut(s) 15, 476
Bbv12I GWGCWC 2 cut(s) 50, 181
BbvI GCAGC 3 cut(s) 295, 328, 433
BccI CCATC 1 cut(s) 218
BciT130I CCWGG 2 cut(s) 206, 251
BclI TGATCA 1 cut(s) 283
BcoDI GTCTC 3 cut(s) 80, 192, 380
BfaI CTAG 2 cut(s) 270, 413
BisI GCNGC 4 cut(s) 309, 316, 342, 422
BlsI GCNGC 4 cut(s) 310, 317, 343, 423
Bme1390I CCNGG 2 cut(s) 206, 251
Bme18I GGWCC 2 cut(s) 67, 80
BmgBI CACGTC 1 cut(s) 74
BmgT120I GGNCC 3 cut(s) 67, 80, 253
BmiI GGNNCC 1 cut(s) 69
BmrFI CCNGG 2 cut(s) 206, 251
BmrI ACTGGG 1 cut(s) 111
BmsI GCATC 2 cut(s) 328, 439
BmuI ACTGGG 1 cut(s) 111
BoxI GACNNNNGTC 1 cut(s) 72
BpiI GAAGAC 2 cut(s) 15, 476
BplI GAGNNNNNCTC 2 cut(s) 185, 217
Bpu14I TTCGAA 1 cut(s) 140
BpuEI CTTGAG 1 cut(s) 454
BsaAI YACGTR 1 cut(s) 93
BsaJI CCNNGG 4 cut(s) 63, 204, 261, 348
Bsc4I CCNNNNNNNGG 4 cut(s) 64, 77, 250, 262
Bse1I ACTGG 1 cut(s) 117
Bse3DI GCAATG 1 cut(s) 292
BseBI CCWGG 2 cut(s) 206, 251
BseDI CCNNGG 4 cut(s) 63, 204, 261, 348
BseLI CCNNNNNNNGG 4 cut(s) 64, 77, 250, 262
BseMI GCAATG 1 cut(s) 292
BseMII CTCAG 3 cut(s) 291, 391, 474
BseNI ACTGG 1 cut(s) 117
BseRI GAGGAG 2 cut(s) 30, 451
BseXI GCAGC 3 cut(s) 295, 328, 433
Bsh1285I CGRYCG 1 cut(s) 32
BshFI GGCC 3 cut(s) 209, 254, 315
BsiEI CGRYCG 1 cut(s) 32
BsiHKAI GWGCWC 2 cut(s) 50, 181
BslI CCNNNNNNNGG 4 cut(s) 64, 77, 250, 262
BsmAI GTCTC 3 cut(s) 80, 192, 380
BsmBI CGTCTC 1 cut(s) 80
BsnI GGCC 3 cut(s) 209, 254, 315
Bsp119I TTCGAA 1 cut(s) 140
Bsp1286I GDGCHC 2 cut(s) 50, 181
Bsp143I GATC 2 cut(s) 112, 283
BspACI CCGC 4 cut(s) 32, 130, 247, 316
BspANI GGCC 3 cut(s) 209, 254, 315
BspCNI CTCAG 3 cut(s) 290, 390, 473
BspLI GGNNCC 1 cut(s) 69
BspPI GGATC 1 cut(s) 107
BspT104I TTCGAA 1 cut(s) 140
BsrDI GCAATG 1 cut(s) 292
BsrI ACTGG 1 cut(s) 117
BssECI CCNNGG 4 cut(s) 63, 204, 261, 348
BssMI GATC 2 cut(s) 112, 283
BssT1I CCWWGG 1 cut(s) 348
Bst2UI CCWGG 2 cut(s) 206, 251
Bst4CI ACNGT 1 cut(s) 87
Bst6I CTCTTC 1 cut(s) 239
BstBAI YACGTR 1 cut(s) 93
BstBI TTCGAA 1 cut(s) 140
BstC8I GCNNGC 1 cut(s) 313
BstDEI CTNAG 3 cut(s) 277, 377, 460
BstENI CCTNNNNNAGG 1 cut(s) 260
BstKTI GATC 2 cut(s) 115, 286
BstMAI GTCTC 3 cut(s) 80, 192, 380
BstMBI GATC 2 cut(s) 112, 283
BstMCI CGRYCG 1 cut(s) 32
BstNI CCWGG 2 cut(s) 206, 251
BstNSI RCATGY 1 cut(s) 292
BstPAI GACNNNNGTC 1 cut(s) 72
BstSCI CCNGG 2 cut(s) 204, 249
BstV1I GCAGC 3 cut(s) 295, 328, 433
BstV2I GAAGAC 2 cut(s) 15, 476
BsuRI GGCC 3 cut(s) 209, 254, 315
BtgZI GCGATG 1 cut(s) 196
BtrI CACGTC 1 cut(s) 74
BtsIMutI CAGTG 2 cut(s) 124, 336
Cac8I GCNNGC 1 cut(s) 313
Cfr13I GGNCC 3 cut(s) 67, 80, 253
CseI GACGC 1 cut(s) 314
CviAII CATG 3 cut(s) 289, 372, 489
DdeI CTNAG 3 cut(s) 277, 377, 460
DpnI GATC 2 cut(s) 114, 285
DpnII GATC 2 cut(s) 112, 283
EaeI YGGCCR 2 cut(s) 207, 313
Eam1104I CTCTTC 1 cut(s) 239
EarI CTCTTC 1 cut(s) 239
EciI GGCGGA 1 cut(s) 236
Ecl136II GAGCTC 2 cut(s) 48, 179
Eco130I CCWWGG 1 cut(s) 348
Eco24I GRGCYC 2 cut(s) 50, 181
Eco47I GGWCC 2 cut(s) 67, 80
Eco53kI GAGCTC 2 cut(s) 48, 179
Eco72I CACGTG 1 cut(s) 93
EcoICRI GAGCTC 2 cut(s) 48, 179
EcoNI CCTNNNNNAGG 1 cut(s) 260
EcoRII CCWGG 2 cut(s) 204, 249
EcoT14I CCWWGG 1 cut(s) 348
EcoT22I ATGCAT 1 cut(s) 294
EcoT38I GRGCYC 2 cut(s) 50, 181
ErhI CCWWGG 1 cut(s) 348
Esp3I CGTCTC 1 cut(s) 80
FaeI CATG 3 cut(s) 292, 375, 492
FaiI YATR 5 cut(s) 290, 363, 365, 373, 490
FatI CATG 3 cut(s) 288, 371, 488
FbaI TGATCA 1 cut(s) 283
Fnu4HI GCNGC 4 cut(s) 309, 316, 342, 422
FriOI GRGCYC 2 cut(s) 50, 181
Fsp4HI GCNGC 4 cut(s) 309, 316, 342, 422
FspBI CTAG 2 cut(s) 270, 413
GluI GCNGC 4 cut(s) 309, 316, 342, 422
HaeIII GGCC 3 cut(s) 209, 254, 315
HgaI GACGC 1 cut(s) 314
Hin1II CATG 3 cut(s) 292, 375, 492
HinfI GANTC 5 cut(s) 101, 137, 200, 326, 458
HphI GGTGA 2 cut(s) 217, 314
Hpy166II GTNNAC 3 cut(s) 83, 90, 469
Hpy188I TCNGA 7 cut(s) 42, 52, 67, 153, 176, 262, 380
Hpy188III TCNNGA 6 cut(s) 98, 182, 193, 323, 433, 455
Hpy8I GTNNAC 3 cut(s) 83, 90, 469
Hpy99I CGWCG 1 cut(s) 46
HpyAV CCTTC 3 cut(s) 329, 391, 488
HpyCH4III ACNGT 1 cut(s) 87
HpyCH4IV ACGT 2 cut(s) 73, 92
HpyCH4V TGCA 2 cut(s) 292, 421
HpyF3I CTNAG 3 cut(s) 277, 377, 460
HpySE526I ACGT 2 cut(s) 73, 92
Hsp92II CATG 3 cut(s) 292, 375, 492
Ksp22I TGATCA 1 cut(s) 283
Kzo9I GATC 2 cut(s) 112, 283
LmnI GCTCC 2 cut(s) 53, 480
LpnPI CCDG 9 cut(s) 111, 130, 173, 191, 218, 236, 263, 297, 447
Lsp1109I GCAGC 3 cut(s) 295, 328, 433
LweI GCATC 2 cut(s) 328, 439
MaeI CTAG 2 cut(s) 270, 413
MaeII ACGT 2 cut(s) 73, 92
MaeIII GTNAC 2 cut(s) 265, 302
MalI GATC 2 cut(s) 114, 285
MboI GATC 2 cut(s) 112, 283
MboII GAAGA 4 cut(s) 20, 226, 401, 476
MhlI GDGCHC 2 cut(s) 50, 181
MlsI TGGCCA 1 cut(s) 209
MluCI AATT 1 cut(s) 367
MluNI TGGCCA 1 cut(s) 209
MlyI GAGTC 2 cut(s) 194, 452
MmeI TCCRAC 1 cut(s) 65
Mox20I TGGCCA 1 cut(s) 209
Mph1103I ATGCAT 1 cut(s) 294
MscI TGGCCA 1 cut(s) 209
MseI TTAA 1 cut(s) 383
Msp20I TGGCCA 1 cut(s) 209
MspR9I CCNGG 2 cut(s) 206, 251
MvaI CCWGG 2 cut(s) 206, 251
NdeII GATC 2 cut(s) 112, 283
NlaIII CATG 3 cut(s) 292, 375, 492
NlaIV GGNNCC 1 cut(s) 69
NmuCI GTSAC 1 cut(s) 302
NsiI ATGCAT 1 cut(s) 294
NspI RCATGY 1 cut(s) 292
NspV TTCGAA 1 cut(s) 140
PcsI WCGNNNNNNNCGW 1 cut(s) 189
PfeI GAWTC 3 cut(s) 101, 137, 326
PkrI GCNGC 4 cut(s) 310, 317, 343, 423
PleI GAGTC 2 cut(s) 194, 452
PmaCI CACGTG 1 cut(s) 93
PmlI CACGTG 1 cut(s) 93
PpsI GAGTC 2 cut(s) 194, 452
Ppu21I YACGTR 1 cut(s) 93
PshAI GACNNNNGTC 1 cut(s) 72
Psp124BI GAGCTC 2 cut(s) 50, 181
Psp6I CCWGG 2 cut(s) 204, 249
PspCI CACGTG 1 cut(s) 93
PspGI CCWGG 2 cut(s) 204, 249
PspN4I GGNNCC 1 cut(s) 69
PspPI GGNCC 3 cut(s) 67, 80, 253
SacI GAGCTC 2 cut(s) 50, 181
SaqAI TTAA 1 cut(s) 383
SatI GCNGC 4 cut(s) 309, 316, 342, 422
Sau3AI GATC 2 cut(s) 112, 283
Sau96I GGNCC 3 cut(s) 67, 80, 253
SchI GAGTC 2 cut(s) 194, 452
ScrFI CCNGG 2 cut(s) 206, 251
SduI GDGCHC 2 cut(s) 50, 181
SfaNI GCATC 2 cut(s) 328, 439
SfuI TTCGAA 1 cut(s) 140
SinI GGWCC 2 cut(s) 67, 80
SmlI CTYRAG 1 cut(s) 433
SmoI CTYRAG 1 cut(s) 433
Sse9I AATT 1 cut(s) 367
SsiI CCGC 4 cut(s) 32, 130, 247, 316
SspMI CTAG 2 cut(s) 270, 413
SstI GAGCTC 2 cut(s) 50, 181
StyD4I CCNGG 2 cut(s) 204, 249
StyI CCWWGG 1 cut(s) 348
TaaI ACNGT 1 cut(s) 87
TaiI ACGT 2 cut(s) 76, 95
TaqI TCGA 6 cut(s) 28, 111, 140, 183, 192, 456
TaqII GACCGA 2 cut(s) 68, 179
TasI AATT 1 cut(s) 367
TauI GCSGC 1 cut(s) 318
TfiI GAWTC 3 cut(s) 101, 137, 326
Tru1I TTAA 1 cut(s) 383
Tru9I TTAA 1 cut(s) 383
TscAI CASTG 2 cut(s) 124, 336
TseFI GTSAC 1 cut(s) 302
TseI GCWGC 3 cut(s) 308, 341, 421
Tsp45I GTSAC 1 cut(s) 302
TspDTI ATGAA 4 cut(s) 170, 360, 402, 410
TspGWI ACGGA 1 cut(s) 27
TspRI CASTG 2 cut(s) 124, 336
VpaK11BI GGWCC 2 cut(s) 67, 80
XagI CCTNNNNNAGG 1 cut(s) 260
XapI RAATTY 1 cut(s) 367
XceI RCATGY 1 cut(s) 292
XspI CTAG 2 cut(s) 270, 413
Zsp2I ATGCAT 1 cut(s) 294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.