AT4G24530

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0008318
Physical Location & Seq
Reverse (-)
12667277 .. 12670222
2946 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G24530.1

Sequence Viewer

Length: 1560 bp
ATGCTCCAAATGAAGCAGCCATTACAGAACCTTAATCAGTCGCAGAAGACAGCTTTAGCTGGTGTTTTCGTGCTCCTTTTCCCGATTCTCTATCCGAATCTCTTCAGCCCTCTTGGTCGCGCCTCTCCTTCTCTCTTCTCCGAATGGAATGCTCCAAGGCCTAGACATTTGAGCCTACTCCAAGGCGCTCTTGATCGACAAATTTCGATCAGACAACAAGTTGAGTTATGGTCTCCCTTGGCAGATCAAGGTTGGAAGCCTTGCACTGAATCTTATAGAGGCGCTTCCTTGCCAGAGAAGTCTGAAGGGTTTCTTCAGGTTTTCCTTGACGGCGGACTAAATCAACAAAGAATGGGAATATGTGATGCTGTTGCTGTTGCCAAGATAATGAACGTTACACTCGTGATTCCACGCCTTGAAGTAAATACTGTTTGGCAAGATTCAAGTTCTTTCACAGATATATTTGATTTGGATCACTTTATAAGTGTCCTTAAAGACGAGGTGCGGATTGTTAGGGAGCTTCCAATACAGTATGCCTGGAGCACTCGTGATTATTACGCTACAGGTATTCGAGCTACTAGGATTAAGACTGCGCCTGTTCACGCTTCAGCAGAGTGGTATTTGGAAAATGTCTTACCTATTATCCAGAGTTATGGCATTGCTGCTGTTGCTCCATTCTCTCACCGTTTGGCATTTGACAATTTGCCTGAAAGTATACAGCGGTTACGCTGTAAAGTAAATTTTGAAGCATTAAACTTTGTTCCTCATATTCGTGAGCTAGGGGACGCTCTTGTGCATCGACTCAGAAATCCTCCTTCCAGCTCTCAAACATCAGGAACTATGGATCCGACAGACAGAATAAACACCATTGTAAAAGCTGGTGCTGGAAAGTTTGCAGTGCTTCATCTTCGTTTCGATAAAGACATGGCTGCTCATTCAGGCTGTGACTTTGAAGGTGGCAAAGCAGAAAAACTGGCGCTGGCAAAATATCGCCAGGTGATCTGGCAAGGAAGGGTCTTAAATTCACAGTTCACAGATGAGGAGCTAAGAAACAAAGGTCGCTGCCCGTTGACACCAGAAGAGATTGGTTTACTGCTGTCAGCTTTGGGTTTCAGTAACAATACCCGTCTCTATCTAGCGTCACACCAGGTTTACGGAGGAGAAGCAAGAATCTCAACGCTGCGTAAACTGTTCCCCGGGATTGAAAACAAGAAAAGCCTAGCCTCTGCTGAGGAACTAGCTGATGTACAAGGGAAAGCTTCTCTGATGGCTGCAGTCGATTACTACGTGAGTATGAAGAGCGACATCTTCATCTCTGCATCTCCCGGGAACATGCACAACGCATTACAGGCGCATAGAGCCTACTTGAATCTCAAAACCATAAGACCCAACATGATACTGTTAGGACAAGTCTTTGTGAACAAGAGCTTAGATTGGTCAGAGTTTGAAGGAGCAGTAATGAATGGGCACAAAAACAGGCAAGGCCAGCTTCGTCTACGCAAACAGAAACAGTCTATTTACACTTATCCAGCTCCTGATTGCATGTGTAAAGTAGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

519

Amino Acids

58.16

Weight (kDa)

9.2

Isoelectric Point (pI)

43.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 106 - 456 1.3e-89 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 482
AccI GTMKAC 2 cut(s) 715, 1495
AccII CGCG 1 cut(s) 120
AciI CCGC 3 cut(s) 333, 505, 721
AclI AACGTT 1 cut(s) 393
AclWI GGATC 3 cut(s) 480, 839, 852
AcsI RAATTY 3 cut(s) 201, 739, 1021
AcuI CTGAAG 4 cut(s) 88, 299, 324, 591
AfaI GTAC 1 cut(s) 1248
AgsI TTSAA 7 cut(s) 419, 444, 746, 953, 1205, 1369, 1448
AjnI CCWGG 3 cut(s) 536, 993, 1146
AloI GAACNNNNNNTCC 2 cut(s) 829, 861
Alw21I GWGCWC 2 cut(s) 75, 545
Alw26I GTCTC 2 cut(s) 237, 1133
AlwI GGATC 3 cut(s) 480, 839, 852
AlwNI CAGNNNCTG 1 cut(s) 1535
Ama87I CYCGRG 2 cut(s) 1196, 1325
AoxI GGCC 2 cut(s) 158, 1483
ApeKI GCWGC 6 cut(s) 16, 662, 929, 1062, 1180, 1271
ApoI RAATTY 3 cut(s) 201, 739, 1021
Asp700I GAANNNNTTC 2 cut(s) 101, 309
AspLEI GCGC 6 cut(s) 122, 188, 284, 595, 979, 1354
AsuC2I CCSGG 4 cut(s) 1197, 1198, 1326, 1327
AsuHPI GGTGA 2 cut(s) 674, 1009
AvaI CYCGRG 2 cut(s) 1196, 1325
BaeGI GKGCMC 1 cut(s) 1470
BamHI GGATCC 1 cut(s) 844
BauI CACGAG 2 cut(s) 401, 546
BbsI GAAGAC 1 cut(s) 53
Bbv12I GWGCWC 2 cut(s) 75, 545
BbvCI CCTCAGC 1 cut(s) 1230
BbvI GCAGC 6 cut(s) 28, 649, 916, 1049, 1167, 1258
BccI CCATC 1 cut(s) 1261
BceAI ACGGC 1 cut(s) 346
BcgI CGANNNNNNTGC 2 cut(s) 131, 165
BciT130I CCWGG 3 cut(s) 538, 995, 1148
BcnI CCSGG 4 cut(s) 1197, 1198, 1326, 1327
BcoDI GTCTC 2 cut(s) 237, 1133
BfaI CTAG 6 cut(s) 162, 579, 779, 1136, 1220, 1238
BfmI CTRYAG 2 cut(s) 561, 1272
BfoI RGCGCY 3 cut(s) 189, 285, 980
BisI GCNGC 6 cut(s) 17, 663, 930, 1063, 1181, 1272
BlsI GCNGC 6 cut(s) 18, 664, 931, 1064, 1182, 1273
Bme1390I CCNGG 7 cut(s) 538, 995, 1148, 1197, 1198, 1326, 1327
BmeT110I CYCGRG 2 cut(s) 1196, 1325
BmiI GGNNCC 1 cut(s) 846
BmrFI CCNGG 7 cut(s) 538, 995, 1148, 1197, 1198, 1326, 1327
BmsI GCATC 3 cut(s) 355, 805, 1328
BpiI GAAGAC 1 cut(s) 53
BpmI CTGGAG 1 cut(s) 559
Bpu10I CCTNAGC 1 cut(s) 1230
BpuMI CCSGG 4 cut(s) 1197, 1198, 1326, 1327
BsaAI YACGTR 1 cut(s) 1288
BsaBI GATNNNNATC 1 cut(s) 471
BsaI GGTCTC 1 cut(s) 237
BsaJI CCNNGG 6 cut(s) 155, 181, 237, 1195, 1196, 1325
BsaXI ACNNNNNCTCC 2 cut(s) 532, 562
Bse1I ACTGG 1 cut(s) 978
Bse3DI GCAATG 1 cut(s) 657
Bse8I GATNNNNATC 1 cut(s) 471
BseBI CCWGG 3 cut(s) 538, 995, 1148
BseDI CCNNGG 6 cut(s) 155, 181, 237, 1195, 1196, 1325
BseJI GATNNNNATC 1 cut(s) 471
BseMI GCAATG 1 cut(s) 657
BseMII CTCAG 2 cut(s) 817, 1221
BseNI ACTGG 1 cut(s) 978
BseRI GAGGAG 2 cut(s) 1055, 1173
BseSI GKGCMC 1 cut(s) 1470
BseXI GCAGC 6 cut(s) 28, 649, 916, 1049, 1167, 1258
Bsh1236I CGCG 1 cut(s) 120
BshFI GGCC 2 cut(s) 160, 1485
BsiHKAI GWGCWC 2 cut(s) 75, 545
BsiHKCI CYCGRG 2 cut(s) 1196, 1325
BsiSI CCGG 2 cut(s) 1197, 1326
BslFI GGGAC 1 cut(s) 797
BsmAI GTCTC 2 cut(s) 237, 1133
BsmBI CGTCTC 1 cut(s) 1133
BsmFI GGGAC 1 cut(s) 797
BsmI GAATGC 1 cut(s) 154
BsnI GGCC 2 cut(s) 160, 1485
Bso31I GGTCTC 1 cut(s) 237
BsoBI CYCGRG 2 cut(s) 1196, 1325
Bsp1286I GDGCHC 3 cut(s) 75, 545, 1470
Bsp1407I TGTACA 1 cut(s) 1246
Bsp143I GATC 6 cut(s) 193, 207, 244, 472, 844, 999
BspACI CCGC 3 cut(s) 333, 505, 721
BspANI GGCC 2 cut(s) 160, 1485
BspCNI CTCAG 2 cut(s) 816, 1222
BspFNI CGCG 1 cut(s) 120
BspLI GGNNCC 1 cut(s) 846
BspMAI CTGCAG 1 cut(s) 1276
BspPI GGATC 3 cut(s) 480, 839, 852
BspQI GCTCTTC 1 cut(s) 1292
BspTNI GGTCTC 1 cut(s) 237
BsrDI GCAATG 1 cut(s) 657
BsrGI TGTACA 1 cut(s) 1246
BsrI ACTGG 1 cut(s) 978
BssECI CCNNGG 6 cut(s) 155, 181, 237, 1195, 1196, 1325
BssMI GATC 6 cut(s) 193, 207, 244, 472, 844, 999
BssNAI GTATAC 1 cut(s) 716
BssSI CACGAG 2 cut(s) 401, 546
BssT1I CCWWGG 3 cut(s) 155, 181, 237
Bst1107I GTATAC 1 cut(s) 716
Bst2BI CACGAG 2 cut(s) 401, 546
Bst2UI CCWGG 3 cut(s) 538, 995, 1148
Bst4CI ACNGT 7 cut(s) 430, 531, 686, 1029, 1191, 1401, 1512
Bst6I CTCTTC 4 cut(s) 107, 140, 1074, 1292
BstAUI TGTACA 1 cut(s) 1246
BstBAI YACGTR 1 cut(s) 1288
BstC8I GCNNGC 2 cut(s) 981, 1487
BstDEI CTNAG 5 cut(s) 803, 1046, 1230, 1429, 1557
BstFNI CGCG 1 cut(s) 120
BstH2I RGCGCY 3 cut(s) 189, 285, 980
BstHHI GCGC 6 cut(s) 122, 188, 284, 595, 979, 1354
BstKTI GATC 6 cut(s) 196, 210, 247, 475, 847, 1002
BstMAI GTCTC 2 cut(s) 237, 1133
BstMBI GATC 6 cut(s) 193, 207, 244, 472, 844, 999
BstMWI GCNNNNNNNGC 4 cut(s) 668, 1349, 1358, 1486
BstNI CCWGG 3 cut(s) 538, 995, 1148
BstNSI RCATGY 2 cut(s) 1336, 1546
BstSCI CCNGG 7 cut(s) 536, 993, 1146, 1195, 1196, 1324, 1325
BstSFI CTRYAG 2 cut(s) 561, 1272
BstSLI GKGCMC 1 cut(s) 1470
BstUI CGCG 1 cut(s) 120
BstV1I GCAGC 6 cut(s) 28, 649, 916, 1049, 1167, 1258
BstV2I GAAGAC 1 cut(s) 53
BstX2I RGATCY 1 cut(s) 844
BstXI CCANNNNNNTGG 1 cut(s) 653
BstYI RGATCY 1 cut(s) 844
BstZ17I GTATAC 1 cut(s) 716
BsuRI GGCC 2 cut(s) 160, 1485
BtsI GCAGTG 1 cut(s) 903
BtsIMutI CAGTG 2 cut(s) 264, 903
Cac8I GCNNGC 2 cut(s) 981, 1487
CaiI CAGNNNCTG 1 cut(s) 1535
CfoI GCGC 6 cut(s) 122, 188, 284, 595, 979, 1354
Cfr9I CCCGGG 2 cut(s) 1196, 1325
CseI GACGC 2 cut(s) 794, 1128
CsiI ACCWGGT 1 cut(s) 1146
Csp6I GTAC 1 cut(s) 1247
CviAII CATG 4 cut(s) 925, 1333, 1393, 1543
CviQI GTAC 1 cut(s) 1247
DdeI CTNAG 5 cut(s) 803, 1046, 1230, 1429, 1557
DpnI GATC 6 cut(s) 195, 209, 246, 474, 846, 1001
DpnII GATC 6 cut(s) 193, 207, 244, 472, 844, 999
Eam1104I CTCTTC 4 cut(s) 107, 140, 1074, 1292
EarI CTCTTC 4 cut(s) 107, 140, 1074, 1292
EciI GGCGGA 1 cut(s) 348
Eco130I CCWWGG 3 cut(s) 155, 181, 237
Eco147I AGGCCT 1 cut(s) 160
Eco31I GGTCTC 1 cut(s) 237
Eco57I CTGAAG 4 cut(s) 88, 299, 324, 591
Eco88I CYCGRG 2 cut(s) 1196, 1325
EcoRII CCWGG 3 cut(s) 536, 993, 1146
EcoT14I CCWWGG 3 cut(s) 155, 181, 237
ErhI CCWWGG 3 cut(s) 155, 181, 237
Esp3I CGTCTC 1 cut(s) 1133
FaeI CATG 4 cut(s) 928, 1336, 1396, 1546
FalI AAGNNNNNCTT 6 cut(s) 174, 206, 297, 329, 1473, 1505
FaqI GGGAC 1 cut(s) 797
FatI CATG 4 cut(s) 924, 1332, 1392, 1542
FblI GTMKAC 2 cut(s) 715, 1495
Fnu4HI GCNGC 6 cut(s) 17, 663, 930, 1063, 1181, 1272
Fsp4HI GCNGC 6 cut(s) 17, 663, 930, 1063, 1181, 1272
FspBI CTAG 6 cut(s) 162, 579, 779, 1136, 1220, 1238
GlaI GCGC 6 cut(s) 121, 187, 283, 594, 978, 1353
GluI GCNGC 6 cut(s) 17, 663, 930, 1063, 1181, 1272
GsuI CTGGAG 1 cut(s) 559
HaeII RGCGCY 3 cut(s) 189, 285, 980
HaeIII GGCC 2 cut(s) 160, 1485
HapII CCGG 2 cut(s) 1197, 1326
HgaI GACGC 2 cut(s) 794, 1128
HhaI GCGC 6 cut(s) 122, 188, 284, 595, 979, 1354
Hin1II CATG 4 cut(s) 928, 1336, 1396, 1546
Hin6I GCGC 6 cut(s) 120, 186, 282, 593, 977, 1352
HinP1I GCGC 6 cut(s) 120, 186, 282, 593, 977, 1352
HincII GTYRAC 1 cut(s) 1071
HindII GTYRAC 1 cut(s) 1071
HindIII AAGCTT 1 cut(s) 1257
HinfI GANTC 8 cut(s) 85, 97, 269, 406, 440, 801, 1170, 1369
HpaII CCGG 2 cut(s) 1197, 1326
HphI GGTGA 2 cut(s) 674, 1009
Hpy166II GTNNAC 9 cut(s) 601, 716, 1032, 1071, 1091, 1153, 1187, 1420, 1496
Hpy188I TCNGA 8 cut(s) 96, 142, 212, 304, 806, 849, 1266, 1441
Hpy188III TCNNGA 8 cut(s) 82, 191, 403, 548, 646, 773, 834, 1535
Hpy8I GTNNAC 9 cut(s) 601, 716, 1032, 1071, 1091, 1153, 1187, 1420, 1496
HpyAV CCTTC 6 cut(s) 138, 299, 825, 947, 1005, 1442
HpyCH4III ACNGT 7 cut(s) 430, 531, 686, 1029, 1191, 1401, 1512
HpyCH4IV ACGT 2 cut(s) 393, 1287
HpyCH4V TGCA 7 cut(s) 264, 796, 896, 1274, 1319, 1336, 1542
HpyF10VI GCNNNNNNNGC 4 cut(s) 668, 1349, 1358, 1486
HpyF3I CTNAG 5 cut(s) 803, 1046, 1230, 1429, 1557
HpySE526I ACGT 2 cut(s) 393, 1287
Hsp92II CATG 4 cut(s) 928, 1336, 1396, 1546
HspAI GCGC 6 cut(s) 120, 186, 282, 593, 977, 1352
Kzo9I GATC 6 cut(s) 193, 207, 244, 472, 844, 999
LguI GCTCTTC 1 cut(s) 1292
LmnI GCTCC 9 cut(s) 9, 78, 157, 517, 540, 676, 1042, 1451, 1537
Lsp1109I GCAGC 6 cut(s) 28, 649, 916, 1049, 1167, 1258
LweI GCATC 3 cut(s) 355, 805, 1328
MabI ACCWGGT 1 cut(s) 1146
MaeI CTAG 6 cut(s) 162, 579, 779, 1136, 1220, 1238
MaeII ACGT 2 cut(s) 393, 1287
MaeIII GTNAC 5 cut(s) 394, 723, 944, 1115, 1140
MalI GATC 6 cut(s) 195, 209, 246, 474, 846, 1001
MboI GATC 6 cut(s) 193, 207, 244, 472, 844, 999
MboII GAAGA 8 cut(s) 58, 94, 127, 305, 899, 1091, 1300, 1309
MflI RGATCY 1 cut(s) 844
MhlI GDGCHC 3 cut(s) 75, 545, 1470
MluCI AATT 4 cut(s) 201, 700, 739, 1021
MlyI GAGTC 1 cut(s) 795
MmeI TCCRAC 2 cut(s) 233, 872
MroXI GAANNNNTTC 2 cut(s) 101, 309
MseI TTAA 5 cut(s) 33, 492, 585, 752, 1019
MslI CAYNNNNRTG 1 cut(s) 771
MspA1I CMGCKG 1 cut(s) 721
MspI CCGG 2 cut(s) 1197, 1326
MspR9I CCNGG 7 cut(s) 538, 995, 1148, 1197, 1198, 1326, 1327
Mva1269I GAATGC 1 cut(s) 154
MvaI CCWGG 3 cut(s) 538, 995, 1148
MvnI CGCG 1 cut(s) 120
MwoI GCNNNNNNNGC 4 cut(s) 668, 1349, 1358, 1486
NciI CCSGG 4 cut(s) 1197, 1198, 1326, 1327
NdeII GATC 6 cut(s) 193, 207, 244, 472, 844, 999
NlaIII CATG 4 cut(s) 928, 1336, 1396, 1546
NlaIV GGNNCC 1 cut(s) 846
NmuCI GTSAC 2 cut(s) 944, 1140
NspI RCATGY 2 cut(s) 1336, 1546
PceI AGGCCT 1 cut(s) 160
PciSI GCTCTTC 1 cut(s) 1292
PcsI WCGNNNNNNNCGW 2 cut(s) 399, 1284
PctI GAATGC 1 cut(s) 154
PdmI GAANNNNTTC 2 cut(s) 101, 309
PfeI GAWTC 7 cut(s) 85, 97, 269, 406, 440, 1170, 1369
PkrI GCNGC 6 cut(s) 18, 664, 931, 1064, 1182, 1273
PleI GAGTC 1 cut(s) 795
PpsI GAGTC 1 cut(s) 795
Ppu21I YACGTR 1 cut(s) 1288
PsiI TTATAA 1 cut(s) 482
Psp1406I AACGTT 1 cut(s) 393
Psp6I CCWGG 3 cut(s) 536, 993, 1146
PspGI CCWGG 3 cut(s) 536, 993, 1146
PspN4I GGNNCC 1 cut(s) 846
PstI CTGCAG 1 cut(s) 1276
PstNI CAGNNNCTG 1 cut(s) 1535
PsuI RGATCY 1 cut(s) 844
RsaI GTAC 1 cut(s) 1248
RsaNI GTAC 1 cut(s) 1247
RseI CAYNNNNRTG 1 cut(s) 771
SapI GCTCTTC 1 cut(s) 1292
SaqAI TTAA 5 cut(s) 33, 492, 585, 752, 1019
SatI GCNGC 6 cut(s) 17, 663, 930, 1063, 1181, 1272
Sau3AI GATC 6 cut(s) 193, 207, 244, 472, 844, 999
SchI GAGTC 1 cut(s) 795
ScrFI CCNGG 7 cut(s) 538, 995, 1148, 1197, 1198, 1326, 1327
SduI GDGCHC 3 cut(s) 75, 545, 1470
SexAI ACCWGGT 1 cut(s) 1146
SfaNI GCATC 3 cut(s) 355, 805, 1328
SfcI CTRYAG 2 cut(s) 561, 1272
SmaI CCCGGG 2 cut(s) 1198, 1327
SmiMI CAYNNNNRTG 1 cut(s) 771
Sse9I AATT 4 cut(s) 201, 700, 739, 1021
SseBI AGGCCT 1 cut(s) 160
SsiI CCGC 3 cut(s) 333, 505, 721
SspMI CTAG 6 cut(s) 162, 579, 779, 1136, 1220, 1238
StuI AGGCCT 1 cut(s) 160
StyD4I CCNGG 7 cut(s) 536, 993, 1146, 1195, 1196, 1324, 1325
StyI CCWWGG 3 cut(s) 155, 181, 237
TaaI ACNGT 7 cut(s) 430, 531, 686, 1029, 1191, 1401, 1512
TaiI ACGT 2 cut(s) 396, 1290
TaqI TCGA 6 cut(s) 196, 206, 571, 799, 915, 1278
TasI AATT 4 cut(s) 201, 700, 739, 1021
TatI WGTACW 1 cut(s) 1246
TfiI GAWTC 7 cut(s) 85, 97, 269, 406, 440, 1170, 1369
Tru1I TTAA 5 cut(s) 33, 492, 585, 752, 1019
Tru9I TTAA 5 cut(s) 33, 492, 585, 752, 1019
TscAI CASTG 2 cut(s) 271, 903
TseFI GTSAC 2 cut(s) 944, 1140
TseI GCWGC 6 cut(s) 16, 662, 929, 1062, 1180, 1271
Tsp45I GTSAC 2 cut(s) 944, 1140
TspDTI ATGAA 6 cut(s) 26, 404, 893, 1300, 1310, 1475
TspGWI ACGGA 1 cut(s) 1170
TspMI CCCGGG 2 cut(s) 1196, 1325
TspRI CASTG 2 cut(s) 271, 903
XapI RAATTY 3 cut(s) 201, 739, 1021
XceI RCATGY 2 cut(s) 1336, 1546
XmaI CCCGGG 2 cut(s) 1196, 1325
XmiI GTMKAC 2 cut(s) 715, 1495
XmnI GAANNNNTTC 2 cut(s) 101, 309
XspI CTAG 6 cut(s) 162, 579, 779, 1136, 1220, 1238
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.