FvH4_5g35380

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0008318
Physical Location & Seq
Reverse (-)
25908259 .. 25911924
3666 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g35380.t3

Sequence Viewer

Length: 1542 bp
ATGAAGCCCTACTACTACTCGCCTAACCATTCTCAGAGAGCCGCCTTGGCTGCCCTCTCCCTCCTTCTCTTCCCCACCTTCTTCCCCAGTCTCTTCGGCCCATTGGGTCGCGCCTCTCCTTCAATCTTCTCTGAATGGAATGCTCCAAAGCCCAGACATTTGCATCTTCTAGAAGCTGCCTTGTCAACCCAATCTTCTGTTGAGCAACAGATCAAGGTTTGGGCTCCTTTGGGAAACCAAGGATGGAAGCATTGCCCTGAAGAGCCTAAAAGTGCTTCATTGAAGCCAAAAGGATCAAATGGGTATATTCAGGTGTTCCTTGATGGAGGCTTGAACCAGCAGAAAATGGGGATTTGTGACGCGGTGGCTGTTGCTAAAATCTTGAACGCAACTCTGGTTCTACCGCACCTTGAAGTTAATCCTGTTTGGCAAGATTCGAGTTCCTTTGAGGAGATTTTTGATGTTGATCACTTCTTAGATGTCCTACGGAATGAAGTTTCTATAGTTAAAGAACTTCCTAGTGAGTTTTCTTGGAGCTCTAGGGAGTATTATGCTACAGGCATACGGCCGACTAGAATTAAGACGGCACCAGTTCACGCTCCTGCTGAGTGGTATGTGGAAAATGTCTTGCCCACAATGCAGAGTTATGGAATTGCTGCTATTTCCCCGTTCTCCCATCGTTTGTCTTTCGACAATATGCCTGAAAGCATCCAGCGCCTACGTTGTAAAGTGAACTTTCAAGCTTTATCCTTTGTTCCTCATATCAGGGAATTGGGAGAAGCCCTTGTTAATCGTCTCCGCAACCCTCCTATAAGAAACCAAAACACAGACTCACAAGAGGGAACAGATGACCTTGATAAACAGGGAGCTGGGAAGTTTGTTGTTTTGCATCTTCGCTTTGACAAAGATATGGCTGCTCATTCCGCCTGTGATTTTGGTGGTGGTAAAGCTGAGAAACTTGCTCTTGCAAAATATCGACAAGTGATTTGGCAGGGAAGGGTCCTAAAATCTCAGTTCACAGATGAAGAGCTCAGAATTCAGGGGCGTTGCCCGTTGACGCCTGAAGAGATTGGATTGTTGCTAGCAGCTCTCGGCTTCAACAATAGCACACGGCTTTATCTTGCTTCCCACAAGGTTTATGGCGGAGAAGCAAGGATCTCAACTCTGAGAAGATTATTTCCACTAATGGAGGATAAGAAGAGCCTTACTTCCGCCAAGGAAAGGGCCAAGGTTGAAGGCAAGGCTTCTCTGTCAGCTGCAGTTGACTATTATGTGAGCATGCAAAGTGACATCTTCATTTCTGCTTCTCCGGGAAACATGCACAATGCACTGGTGTGTCATCGAGCTTATATGAACTTAAAGACTATTAGACCAAGCATGACCCTGTTGAGTAAGCTATTCTTGAATAAGAGCATGCAGTGGTCAGAGTTCCAACAGGCAGTTTTGGACGGGCATAAAAACAGACAAGGGCAGATCAGGTTGAGAAAGGAGAAGCAATCCATATACACATATCCTGCTCCTGATTGCATGTGTCAAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

514

Amino Acids

57.65

Weight (kDa)

9.12

Isoelectric Point (pI)

48.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 2 - 336 7e-82 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 586
AccII CGCG 2 cut(s) 111, 362
AciI CCGC 7 cut(s) 42, 362, 404, 799, 924, 1143, 1212
AclWI GGATC 2 cut(s) 301, 1163
AcoI YGGCCR 1 cut(s) 566
AcsI RAATTY 1 cut(s) 1035
AcuI CTGAAG 2 cut(s) 279, 1083
AcyI GRCGYC 1 cut(s) 1058
AfiI CCNNNNNNNGG 1 cut(s) 1221
AgsI TTSAA 9 cut(s) 123, 283, 334, 385, 413, 740, 1099, 1235, 1405
AleI CACNNNNGTG 1 cut(s) 1333
Alw21I GWGCWC 2 cut(s) 539, 1032
Alw26I GTCTC 2 cut(s) 95, 800
AlwI GGATC 2 cut(s) 301, 1163
AoxI GGCC 3 cut(s) 97, 566, 1224
ApeKI GCWGC 6 cut(s) 50, 176, 656, 914, 1085, 1256
ApoI RAATTY 1 cut(s) 1035
ArsI GACNNNNNNTTYG 2 cut(s) 969, 1001
AspLEI GCGC 2 cut(s) 113, 717
AspS9I GGNCC 3 cut(s) 98, 1000, 1224
AsuC2I CCSGG 1 cut(s) 1311
AsuNHI GCTAGC 1 cut(s) 1081
AvaII GGWCC 1 cut(s) 1000
BanI GGYRCC 1 cut(s) 586
BanII GRGCYC 3 cut(s) 226, 539, 1032
BarI GAAGNNNNNNTAC 1 cut(s) 28
Bbv12I GWGCWC 2 cut(s) 539, 1032
BbvI GCAGC 6 cut(s) 37, 163, 643, 901, 1097, 1243
BccI CCATC 3 cut(s) 237, 317, 684
BceAI ACGGC 3 cut(s) 581, 600, 1127
BclI TGATCA 1 cut(s) 466
BcnI CCSGG 1 cut(s) 1311
BcoDI GTCTC 2 cut(s) 95, 800
BfaI CTAG 5 cut(s) 170, 519, 540, 573, 1082
BfmI CTRYAG 3 cut(s) 501, 555, 1257
BfoI RGCGCY 1 cut(s) 718
BglI GCCNNNNNGGC 1 cut(s) 47
BisI GCNGC 7 cut(s) 42, 51, 177, 657, 915, 1086, 1257
BlsI GCNGC 7 cut(s) 43, 52, 178, 658, 916, 1087, 1258
Bme1390I CCNGG 1 cut(s) 1311
Bme18I GGWCC 1 cut(s) 1000
BmgT120I GGNCC 3 cut(s) 98, 1000, 1224
BmiI GGNNCC 3 cut(s) 225, 588, 1001
BmrFI CCNGG 1 cut(s) 1311
BmrI ACTGGG 1 cut(s) 81
BmsI GCATC 3 cut(s) 172, 717, 898
BmtI GCTAGC 1 cut(s) 1085
BmuI ACTGGG 1 cut(s) 81
BpuMI CCSGG 1 cut(s) 1311
BsaBI GATNNNNATC 1 cut(s) 465
BsaHI GRCGYC 1 cut(s) 1058
BsaJI CCNNGG 4 cut(s) 45, 238, 1215, 1227
Bsc4I CCNNNNNNNGG 1 cut(s) 1221
Bse1I ACTGG 3 cut(s) 87, 590, 1335
Bse3DI GCAATG 1 cut(s) 250
Bse8I GATNNNNATC 1 cut(s) 465
BseDI CCNNGG 4 cut(s) 45, 238, 1215, 1227
BseGI GGATG 2 cut(s) 248, 708
BseJI GATNNNNATC 1 cut(s) 465
BseLI CCNNNNNNNGG 1 cut(s) 1221
BseMI GCAATG 1 cut(s) 250
BseMII CTCAG 6 cut(s) 47, 597, 942, 1025, 1045, 1157
BseNI ACTGG 3 cut(s) 87, 590, 1335
BseRI GAGGAG 1 cut(s) 464
BseX3I CGGCCG 1 cut(s) 566
BseXI GCAGC 6 cut(s) 37, 163, 643, 901, 1097, 1243
BseYI CCCAGC 1 cut(s) 869
Bsh1236I CGCG 2 cut(s) 111, 362
Bsh1285I CGRYCG 1 cut(s) 569
BshFI GGCC 3 cut(s) 99, 568, 1226
BshNI GGYRCC 1 cut(s) 586
BsiEI CGRYCG 1 cut(s) 569
BsiHKAI GWGCWC 2 cut(s) 539, 1032
BsiSI CCGG 1 cut(s) 1310
BslI CCNNNNNNNGG 1 cut(s) 1221
BsmAI GTCTC 2 cut(s) 95, 800
BsmBI CGTCTC 1 cut(s) 800
BsmI GAATGC 1 cut(s) 145
BsnI GGCC 3 cut(s) 99, 568, 1226
Bsp1286I GDGCHC 3 cut(s) 226, 539, 1032
Bsp143I GATC 5 cut(s) 210, 293, 466, 1155, 1473
BspACI CCGC 7 cut(s) 42, 362, 404, 799, 924, 1143, 1212
BspANI GGCC 3 cut(s) 99, 568, 1226
BspCNI CTCAG 6 cut(s) 46, 598, 943, 1024, 1044, 1158
BspFNI CGCG 2 cut(s) 111, 362
BspLI GGNNCC 3 cut(s) 225, 588, 1001
BspMAI CTGCAG 1 cut(s) 1261
BspOI GCTAGC 1 cut(s) 1085
BspPI GGATC 2 cut(s) 301, 1163
BspQI GCTCTTC 3 cut(s) 255, 1020, 1193
BspT107I GGYRCC 1 cut(s) 586
BsrDI GCAATG 1 cut(s) 250
BsrI ACTGG 3 cut(s) 87, 590, 1335
BssECI CCNNGG 4 cut(s) 45, 238, 1215, 1227
BssMI GATC 5 cut(s) 210, 293, 466, 1155, 1473
BssNI GRCGYC 1 cut(s) 1058
BssT1I CCWWGG 4 cut(s) 45, 238, 1215, 1227
Bst6I CTCTTC 6 cut(s) 74, 98, 255, 1020, 1059, 1193
BstACI GRCGYC 1 cut(s) 1058
BstC8I GCNNGC 3 cut(s) 1083, 1280, 1415
BstDEI CTNAG 7 cut(s) 33, 475, 606, 951, 1011, 1031, 1166
BstF5I GGATG 2 cut(s) 248, 708
BstFNI CGCG 2 cut(s) 111, 362
BstH2I RGCGCY 1 cut(s) 718
BstHHI GCGC 2 cut(s) 113, 717
BstKTI GATC 5 cut(s) 213, 296, 469, 1158, 1476
BstMAI GTCTC 2 cut(s) 95, 800
BstMBI GATC 5 cut(s) 210, 293, 466, 1155, 1473
BstMCI CGRYCG 1 cut(s) 569
BstMWI GCNNNNNNNGC 5 cut(s) 47, 50, 637, 714, 923
BstNSI RCATGY 4 cut(s) 1282, 1321, 1417, 1531
BstSCI CCNGG 1 cut(s) 1309
BstSFI CTRYAG 3 cut(s) 501, 555, 1257
BstUI CGCG 2 cut(s) 111, 362
BstV1I GCAGC 6 cut(s) 37, 163, 643, 901, 1097, 1243
BstX2I RGATCY 1 cut(s) 1155
BstYI RGATCY 1 cut(s) 1155
BstZI CGGCCG 1 cut(s) 566
BsuRI GGCC 3 cut(s) 99, 568, 1226
BtsCI GGATG 2 cut(s) 248, 708
BtsI GCAGTG 1 cut(s) 1424
BtsIMutI CAGTG 2 cut(s) 1328, 1424
Cac8I GCNNGC 3 cut(s) 1083, 1280, 1415
CfoI GCGC 2 cut(s) 113, 717
Cfr13I GGNCC 3 cut(s) 98, 1000, 1224
CseI GACGC 2 cut(s) 368, 1066
CviAII CATG 5 cut(s) 1279, 1318, 1378, 1414, 1528
DdeI CTNAG 7 cut(s) 33, 475, 606, 951, 1011, 1031, 1166
DpnI GATC 5 cut(s) 212, 295, 468, 1157, 1475
DpnII GATC 5 cut(s) 210, 293, 466, 1155, 1473
EaeI YGGCCR 1 cut(s) 566
EagI CGGCCG 1 cut(s) 566
Eam1104I CTCTTC 6 cut(s) 74, 98, 255, 1020, 1059, 1193
EarI CTCTTC 6 cut(s) 74, 98, 255, 1020, 1059, 1193
EciI GGCGGA 3 cut(s) 913, 1158, 1201
Ecl136II GAGCTC 2 cut(s) 537, 1030
EclXI CGGCCG 1 cut(s) 566
Eco130I CCWWGG 4 cut(s) 45, 238, 1215, 1227
Eco24I GRGCYC 3 cut(s) 226, 539, 1032
Eco47I GGWCC 1 cut(s) 1000
Eco52I CGGCCG 1 cut(s) 566
Eco53kI GAGCTC 2 cut(s) 537, 1030
Eco57I CTGAAG 2 cut(s) 279, 1083
EcoICRI GAGCTC 2 cut(s) 537, 1030
EcoO109I RGGNCCY 1 cut(s) 1000
EcoRI GAATTC 1 cut(s) 1035
EcoT14I CCWWGG 4 cut(s) 45, 238, 1215, 1227
EcoT38I GRGCYC 3 cut(s) 226, 539, 1032
ErhI CCWWGG 4 cut(s) 45, 238, 1215, 1227
Esp3I CGTCTC 1 cut(s) 800
FaeI CATG 5 cut(s) 1282, 1321, 1381, 1417, 1531
FalI AAGNNNNNCTT 2 cut(s) 1385, 1417
FatI CATG 5 cut(s) 1278, 1317, 1377, 1413, 1527
FbaI TGATCA 1 cut(s) 466
Fnu4HI GCNGC 7 cut(s) 42, 51, 177, 657, 915, 1086, 1257
FokI GGATG 2 cut(s) 255, 695
FriOI GRGCYC 3 cut(s) 226, 539, 1032
Fsp4HI GCNGC 7 cut(s) 42, 51, 177, 657, 915, 1086, 1257
FspBI CTAG 5 cut(s) 170, 519, 540, 573, 1082
GlaI GCGC 2 cut(s) 112, 716
GluI GCNGC 7 cut(s) 42, 51, 177, 657, 915, 1086, 1257
GsaI CCCAGC 1 cut(s) 873
HaeII RGCGCY 1 cut(s) 718
HaeIII GGCC 3 cut(s) 99, 568, 1226
HapII CCGG 1 cut(s) 1310
HgaI GACGC 2 cut(s) 368, 1066
HhaI GCGC 2 cut(s) 113, 717
Hin1I GRCGYC 1 cut(s) 1058
Hin1II CATG 5 cut(s) 1282, 1321, 1381, 1417, 1531
Hin6I GCGC 2 cut(s) 111, 715
HinP1I GCGC 2 cut(s) 111, 715
HincII GTYRAC 3 cut(s) 186, 1056, 1264
HindII GTYRAC 3 cut(s) 186, 1056, 1264
HindIII AAGCTT 2 cut(s) 741, 1536
HinfI GANTC 2 cut(s) 434, 830
HpaII CCGG 1 cut(s) 1310
Hpy166II GTNNAC 6 cut(s) 186, 595, 733, 1017, 1056, 1264
Hpy188I TCNGA 5 cut(s) 36, 133, 1034, 1167, 1426
Hpy188III TCNNGA 4 cut(s) 170, 382, 1402, 1520
Hpy8I GTNNAC 6 cut(s) 186, 595, 733, 1017, 1056, 1264
HpyAV CCTTC 5 cut(s) 74, 88, 129, 990, 1229
HpyCH4IV ACGT 1 cut(s) 721
HpyF10VI GCNNNNNNNGC 5 cut(s) 47, 50, 637, 714, 923
HpyF3I CTNAG 7 cut(s) 33, 475, 606, 951, 1011, 1031, 1166
HpySE526I ACGT 1 cut(s) 721
Hsp92I GRCGYC 1 cut(s) 1058
Hsp92II CATG 5 cut(s) 1282, 1321, 1381, 1417, 1531
HspAI GCGC 2 cut(s) 111, 715
Ksp22I TGATCA 1 cut(s) 466
Kzo9I GATC 5 cut(s) 210, 293, 466, 1155, 1473
LguI GCTCTTC 3 cut(s) 255, 1020, 1193
LmnI GCTCC 6 cut(s) 148, 229, 534, 604, 866, 1522
Lsp1109I GCAGC 6 cut(s) 37, 163, 643, 901, 1097, 1243
LweI GCATC 3 cut(s) 172, 717, 898
MaeI CTAG 5 cut(s) 170, 519, 540, 573, 1082
MaeII ACGT 1 cut(s) 721
MaeIII GTNAC 2 cut(s) 356, 1286
MalI GATC 5 cut(s) 212, 295, 468, 1157, 1475
MboI GATC 5 cut(s) 210, 293, 466, 1155, 1473
MflI RGATCY 1 cut(s) 1155
MhlI GDGCHC 3 cut(s) 226, 539, 1032
MluCI AATT 4 cut(s) 576, 651, 770, 1035
MlyI GAGTC 1 cut(s) 824
MmeI TCCRAC 1 cut(s) 1456
MnlI CCTC 9 cut(s) 65, 71, 124, 320, 442, 768, 816, 832, 1183
MseI TTAA 6 cut(s) 417, 507, 579, 789, 1358, 1540
MslI CAYNNNNRTG 1 cut(s) 1333
MspA1I CMGCKG 1 cut(s) 1256
MspI CCGG 1 cut(s) 1310
MspR9I CCNGG 1 cut(s) 1311
Mva1269I GAATGC 1 cut(s) 145
MvnI CGCG 2 cut(s) 111, 362
MwoI GCNNNNNNNGC 5 cut(s) 47, 50, 637, 714, 923
NciI CCSGG 1 cut(s) 1311
NdeII GATC 5 cut(s) 210, 293, 466, 1155, 1473
NheI GCTAGC 1 cut(s) 1081
NlaIII CATG 5 cut(s) 1282, 1321, 1381, 1417, 1531
NlaIV GGNNCC 3 cut(s) 225, 588, 1001
NmeAIII GCCGAG 1 cut(s) 1071
NmuCI GTSAC 2 cut(s) 356, 1286
NspI RCATGY 4 cut(s) 1282, 1321, 1417, 1531
OliI CACNNNNGTG 1 cut(s) 1333
PaeI GCATGC 2 cut(s) 1282, 1417
PciSI GCTCTTC 3 cut(s) 255, 1020, 1193
PctI GAATGC 1 cut(s) 145
PfeI GAWTC 1 cut(s) 434
PfoI TCCNGGA 1 cut(s) 1309
PkrI GCNGC 7 cut(s) 43, 52, 178, 658, 916, 1087, 1258
PleI GAGTC 1 cut(s) 824
PpsI GAGTC 1 cut(s) 824
PpuMI RGGWCCY 1 cut(s) 1000
Psp124BI GAGCTC 2 cut(s) 539, 1032
Psp5II RGGWCCY 1 cut(s) 1000
PspFI CCCAGC 1 cut(s) 869
PspN4I GGNNCC 3 cut(s) 225, 588, 1001
PspPI GGNCC 3 cut(s) 98, 1000, 1224
PspPPI RGGWCCY 1 cut(s) 1000
PstI CTGCAG 1 cut(s) 1261
PsuI RGATCY 1 cut(s) 1155
PvuII CAGCTG 1 cut(s) 1256
RseI CAYNNNNRTG 1 cut(s) 1333
SacI GAGCTC 2 cut(s) 539, 1032
SapI GCTCTTC 3 cut(s) 255, 1020, 1193
SaqAI TTAA 6 cut(s) 417, 507, 579, 789, 1358, 1540
SatI GCNGC 7 cut(s) 42, 51, 177, 657, 915, 1086, 1257
Sau3AI GATC 5 cut(s) 210, 293, 466, 1155, 1473
Sau96I GGNCC 3 cut(s) 98, 1000, 1224
SchI GAGTC 1 cut(s) 824
ScrFI CCNGG 1 cut(s) 1311
SduI GDGCHC 3 cut(s) 226, 539, 1032
SfaNI GCATC 3 cut(s) 172, 717, 898
SfcI CTRYAG 3 cut(s) 501, 555, 1257
SinI GGWCC 1 cut(s) 1000
SmiMI CAYNNNNRTG 1 cut(s) 1333
SphI GCATGC 2 cut(s) 1282, 1417
Sse9I AATT 4 cut(s) 576, 651, 770, 1035
SsiI CCGC 7 cut(s) 42, 362, 404, 799, 924, 1143, 1212
SspMI CTAG 5 cut(s) 170, 519, 540, 573, 1082
SstI GAGCTC 2 cut(s) 539, 1032
StyD4I CCNGG 1 cut(s) 1309
StyI CCWWGG 4 cut(s) 45, 238, 1215, 1227
TaiI ACGT 1 cut(s) 724
TaqI TCGA 4 cut(s) 437, 690, 976, 1342
TasI AATT 4 cut(s) 576, 651, 770, 1035
TauI GCSGC 1 cut(s) 44
TfiI GAWTC 1 cut(s) 434
Tru1I TTAA 6 cut(s) 417, 507, 579, 789, 1358, 1540
Tru9I TTAA 6 cut(s) 417, 507, 579, 789, 1358, 1540
TscAI CASTG 2 cut(s) 1335, 1424
TseFI GTSAC 2 cut(s) 356, 1286
TseI GCWGC 6 cut(s) 50, 176, 656, 914, 1085, 1256
Tsp45I GTSAC 2 cut(s) 356, 1286
TspDTI ATGAA 6 cut(s) 17, 267, 507, 1038, 1285, 1367
TspGWI ACGGA 1 cut(s) 502
TspRI CASTG 2 cut(s) 1335, 1424
VpaK11BI GGWCC 1 cut(s) 1000
XapI RAATTY 1 cut(s) 1035
XbaI TCTAGA 1 cut(s) 169
XceI RCATGY 4 cut(s) 1282, 1321, 1417, 1531
XcmI CCANNNNNNNNNTGG 1 cut(s) 1136
XspI CTAG 5 cut(s) 170, 519, 540, 573, 1082
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.