MD08G1197500.v1.1

At1g04910-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0008318
Physical Location & Seq
Forward (+)
25617984 .. 25621880
3897 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1197500.v1.1.491

Sequence Viewer

Length: 1104 bp
ATGAAGCTTCACAAGGACTACTATGGCTACTGCTACCCCAACCAGTCTCAGAAAACCGCTTTAACTGGCCTCCTGGTGGTCCTCTTTCCAGCCTTCCTGCCAAACCTCTTTGGCCCATTAAGCCACACTGCTCATTCCATCTTCTCGTCTGTGGATCAACAGAGCAAGCTTTGGGCTCCGTTGCCCAACCACGGATGGAAGCATTGCCCTGAAGAGCCTAATAGTTTATCATCATCGCCAAAAGAATCTACCGGGTATATTCAGGTGTTCCTTGACGAAGGCTTGAACCAGCAGAAAATGGGGATTTGTGATGCAGTTGCTGTTGCTGTTGCTAAAATTTTGAATGCCACTTTGGTTATTCCACACCTTGAAGTTAATCCTGTGTGGCAAGATTCGAGCTCCTTTGAGGACATTTTTGATGTGGATCACTTTATTGAAGCCCTACAGGGTGAAGTTTCTATAGTTAAAGCCCTTCCTAGTGAATTTTCTTGGAGCACTAGGGAGTATTATGCTACTGGCATAAGGATTACTAGAATAAAAACAGCACCTGTTCACGCTTCTTCTGATTGGTATCTGGAAAACGTCTTGCCCTTATTGCAAAGATATGGAGTTGCTGCTATCTCCCCATTTTCTCATCGTTTGGCCTTTGACAACTTGCCTCAAAACATACAGCGCGTAAGTTGTAAAGTCAACTTTCAAGCTTTAGCCTTTGTTCCTCATATCAGGGAATTGGGAGAAACCCTTGTTAATCGTCTCCGCTACCCTCCCAATGGAAACCCCAAAGGAGGTAGTGACTCACAGGACAAGGCAAATGAAATTGAGAAACAAGGAGCTGGGAAATATGTTGTTTTGCATCTTCGCTTCAATAAAGACATAGCTGCTCATTCAGCGTGTGATTTTGGTGGAGGTAAAGCTGAGAAACTCGCCCTTGCAAAATATCGGCAAGTAATCTGGCAGGGTAGGGTCCTAAATTCTCAGTTCACAGATGAGGAGTTAAGAAATCAAGGGCGTTGCCCATTGACTCCTGAAGAGATTGGATTGCTGCTGGCAGCTTTGGGCTTCAACAATACCACCCGTCTCTATCTTGCTTCTTACAAGGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

368

Amino Acids

40.99

Weight (kDa)

6.86

Isoelectric Point (pI)

47.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 86 - 365 9.5e-65 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 675
AciI CCGC 2 cut(s) 57, 757
AclWI GGATC 2 cut(s) 162, 432
AcsI RAATTY 3 cut(s) 336, 482, 970
AcuI CTGAAG 2 cut(s) 231, 1047
AfiI CCNNNNNNNGG 4 cut(s) 76, 191, 770, 785
AgsI TTSAA 7 cut(s) 286, 343, 371, 437, 698, 865, 1063
AjnI CCWGG 1 cut(s) 72
AjuI GAANNNNNNNTTGG 2 cut(s) 335, 367
AluBI AGCT 8 cut(s) 7, 169, 399, 701, 833, 878, 914, 1052
AluI AGCT 8 cut(s) 7, 169, 399, 701, 833, 878, 914, 1052
Alw21I GWGCWC 2 cut(s) 401, 497
Alw26I GTCTC 3 cut(s) 51, 758, 1082
AlwI GGATC 2 cut(s) 162, 432
AlwNI CAGNNNCTG 2 cut(s) 320, 548
AoxI GGCC 3 cut(s) 67, 112, 642
ApeKI GCWGC 4 cut(s) 614, 878, 1042, 1049
ApoI RAATTY 3 cut(s) 336, 482, 970
AspLEI GCGC 1 cut(s) 675
AspS9I GGNCC 3 cut(s) 79, 113, 964
AsuC2I CCSGG 1 cut(s) 253
AsuHPI GGTGA 1 cut(s) 461
AvaII GGWCC 2 cut(s) 79, 964
BanII GRGCYC 2 cut(s) 178, 401
Bbv12I GWGCWC 2 cut(s) 401, 497
BbvI GCAGC 4 cut(s) 601, 865, 1029, 1061
BccI CCATC 2 cut(s) 146, 189
BciT130I CCWGG 1 cut(s) 74
BcnI CCSGG 1 cut(s) 253
BcoDI GTCTC 3 cut(s) 51, 758, 1082
BfaI CTAG 3 cut(s) 477, 498, 531
BfmI CTRYAG 2 cut(s) 443, 459
BisI GCNGC 4 cut(s) 615, 879, 1043, 1050
BlsI GCNGC 4 cut(s) 616, 880, 1044, 1051
Bme1390I CCNGG 2 cut(s) 74, 253
Bme18I GGWCC 2 cut(s) 79, 964
BmgT120I GGNCC 3 cut(s) 79, 113, 964
BmiI GGNNCC 2 cut(s) 177, 965
BmrFI CCNGG 2 cut(s) 74, 253
BmsI GCATC 2 cut(s) 301, 862
BpuMI CCSGG 1 cut(s) 253
BsaBI GATNNNNATC 2 cut(s) 423, 570
BsaJI CCNNGG 1 cut(s) 190
Bsc4I CCNNNNNNNGG 4 cut(s) 76, 191, 770, 785
Bse1I ACTGG 3 cut(s) 43, 70, 520
Bse3DI GCAATG 1 cut(s) 202
Bse8I GATNNNNATC 2 cut(s) 423, 570
BseBI CCWGG 1 cut(s) 74
BseDI CCNNGG 1 cut(s) 190
BseGI GGATG 1 cut(s) 200
BseJI GATNNNNATC 2 cut(s) 423, 570
BseLI CCNNNNNNNGG 4 cut(s) 76, 191, 770, 785
BseMI GCAATG 1 cut(s) 202
BseMII CTCAG 3 cut(s) 62, 906, 989
BseNI ACTGG 3 cut(s) 43, 70, 520
BseRI GAGGAG 1 cut(s) 1004
BseXI GCAGC 4 cut(s) 601, 865, 1029, 1061
BseYI CCCAGC 1 cut(s) 833
Bsh1236I CGCG 1 cut(s) 675
BshFI GGCC 3 cut(s) 69, 114, 644
BsiHKAI GWGCWC 2 cut(s) 401, 497
BsiSI CCGG 1 cut(s) 252
BslI CCNNNNNNNGG 4 cut(s) 76, 191, 770, 785
BsmAI GTCTC 3 cut(s) 51, 758, 1082
BsmBI CGTCTC 2 cut(s) 758, 1082
BsmI GAATGC 1 cut(s) 349
BsnI GGCC 3 cut(s) 69, 114, 644
Bsp1286I GDGCHC 3 cut(s) 178, 401, 497
Bsp143I GATC 2 cut(s) 154, 424
BspACI CCGC 2 cut(s) 57, 757
BspANI GGCC 3 cut(s) 69, 114, 644
BspCNI CTCAG 3 cut(s) 61, 907, 988
BspFNI CGCG 1 cut(s) 675
BspLI GGNNCC 2 cut(s) 177, 965
BspPI GGATC 2 cut(s) 162, 432
BspQI GCTCTTC 1 cut(s) 207
BsrDI GCAATG 1 cut(s) 202
BsrI ACTGG 3 cut(s) 43, 70, 520
BssECI CCNNGG 1 cut(s) 190
BssMI GATC 2 cut(s) 154, 424
Bst2UI CCWGG 1 cut(s) 74
Bst6I CTCTTC 2 cut(s) 207, 1023
BstC8I GCNNGC 2 cut(s) 167, 1047
BstDEI CTNAG 3 cut(s) 48, 915, 975
BstDSI CCRYGG 1 cut(s) 190
BstF5I GGATG 1 cut(s) 200
BstFNI CGCG 1 cut(s) 675
BstHHI GCGC 1 cut(s) 675
BstKTI GATC 2 cut(s) 157, 427
BstMAI GTCTC 3 cut(s) 51, 758, 1082
BstMBI GATC 2 cut(s) 154, 424
BstMWI GCNNNNNNNGC 3 cut(s) 120, 595, 887
BstNI CCWGG 1 cut(s) 74
BstSCI CCNGG 2 cut(s) 72, 251
BstSFI CTRYAG 2 cut(s) 443, 459
BstUI CGCG 1 cut(s) 675
BstV1I GCAGC 4 cut(s) 601, 865, 1029, 1061
BsuRI GGCC 3 cut(s) 69, 114, 644
BtgI CCRYGG 1 cut(s) 190
BtgZI GCGATG 1 cut(s) 219
BtsCI GGATG 1 cut(s) 200
BtsI GCAGTG 1 cut(s) 126
BtsIMutI CAGTG 1 cut(s) 126
Cac8I GCNNGC 2 cut(s) 167, 1047
CaiI CAGNNNCTG 2 cut(s) 320, 548
CfoI GCGC 1 cut(s) 675
Cfr13I GGNCC 3 cut(s) 79, 113, 964
DdeI CTNAG 3 cut(s) 48, 915, 975
DpnI GATC 2 cut(s) 156, 426
DpnII GATC 2 cut(s) 154, 424
Eam1104I CTCTTC 2 cut(s) 207, 1023
EarI CTCTTC 2 cut(s) 207, 1023
Ecl136II GAGCTC 1 cut(s) 399
Eco24I GRGCYC 2 cut(s) 178, 401
Eco47I GGWCC 2 cut(s) 79, 964
Eco53kI GAGCTC 1 cut(s) 399
Eco57I CTGAAG 2 cut(s) 231, 1047
EcoICRI GAGCTC 1 cut(s) 399
EcoO109I RGGNCCY 1 cut(s) 964
EcoRII CCWGG 1 cut(s) 72
EcoT38I GRGCYC 2 cut(s) 178, 401
Esp3I CGTCTC 2 cut(s) 758, 1082
Fnu4HI GCNGC 4 cut(s) 615, 879, 1043, 1050
FokI GGATG 1 cut(s) 207
FriOI GRGCYC 2 cut(s) 178, 401
Fsp4HI GCNGC 4 cut(s) 615, 879, 1043, 1050
FspBI CTAG 3 cut(s) 477, 498, 531
GlaI GCGC 1 cut(s) 674
GluI GCNGC 4 cut(s) 615, 879, 1043, 1050
GsaI CCCAGC 1 cut(s) 837
HaeIII GGCC 3 cut(s) 69, 114, 644
HapII CCGG 1 cut(s) 252
HhaI GCGC 1 cut(s) 675
Hin6I GCGC 1 cut(s) 673
HinP1I GCGC 1 cut(s) 673
HincII GTYRAC 1 cut(s) 691
HindII GTYRAC 1 cut(s) 691
HindIII AAGCTT 3 cut(s) 5, 167, 699
HinfI GANTC 4 cut(s) 245, 392, 794, 1021
HpaII CCGG 1 cut(s) 252
HphI GGTGA 1 cut(s) 461
Hpy166II GTNNAC 3 cut(s) 553, 691, 981
Hpy188I TCNGA 2 cut(s) 51, 565
Hpy188III TCNNGA 2 cut(s) 575, 1025
Hpy8I GTNNAC 3 cut(s) 553, 691, 981
HpyAV CCTTC 3 cut(s) 103, 272, 482
HpyCH4IV ACGT 1 cut(s) 582
HpyCH4V TGCA 4 cut(s) 314, 598, 853, 932
HpyF10VI GCNNNNNNNGC 3 cut(s) 120, 595, 887
HpyF3I CTNAG 3 cut(s) 48, 915, 975
HpySE526I ACGT 1 cut(s) 582
HspAI GCGC 1 cut(s) 673
Kzo9I GATC 2 cut(s) 154, 424
LguI GCTCTTC 1 cut(s) 207
LmnI GCTCC 4 cut(s) 181, 404, 492, 830
Lsp1109I GCAGC 4 cut(s) 601, 865, 1029, 1061
LweI GCATC 2 cut(s) 301, 862
MaeI CTAG 3 cut(s) 477, 498, 531
MaeII ACGT 1 cut(s) 582
MaeIII GTNAC 1 cut(s) 791
MalI GATC 2 cut(s) 156, 426
MboI GATC 2 cut(s) 154, 424
MboII GAAGA 5 cut(s) 133, 224, 552, 848, 1040
MhlI GDGCHC 3 cut(s) 178, 401, 497
MluCI AATT 5 cut(s) 336, 482, 728, 816, 970
MlyI GAGTC 2 cut(s) 788, 1015
MseI TTAA 7 cut(s) 62, 119, 375, 465, 747, 995, 1102
MspI CCGG 1 cut(s) 252
MspR9I CCNGG 2 cut(s) 74, 253
Mva1269I GAATGC 1 cut(s) 349
MvaI CCWGG 1 cut(s) 74
MvnI CGCG 1 cut(s) 675
MwoI GCNNNNNNNGC 3 cut(s) 120, 595, 887
NciI CCSGG 1 cut(s) 253
NdeII GATC 2 cut(s) 154, 424
NlaIV GGNNCC 2 cut(s) 177, 965
NmuCI GTSAC 1 cut(s) 791
PciSI GCTCTTC 1 cut(s) 207
PctI GAATGC 1 cut(s) 349
PfeI GAWTC 2 cut(s) 245, 392
PkrI GCNGC 4 cut(s) 616, 880, 1044, 1051
PleI GAGTC 2 cut(s) 788, 1015
PpsI GAGTC 2 cut(s) 788, 1015
PpuMI RGGWCCY 1 cut(s) 964
Psp124BI GAGCTC 1 cut(s) 401
Psp5II RGGWCCY 1 cut(s) 964
Psp6I CCWGG 1 cut(s) 72
PspFI CCCAGC 1 cut(s) 833
PspGI CCWGG 1 cut(s) 72
PspN4I GGNNCC 2 cut(s) 177, 965
PspPI GGNCC 3 cut(s) 79, 113, 964
PspPPI RGGWCCY 1 cut(s) 964
PstNI CAGNNNCTG 2 cut(s) 320, 548
SacI GAGCTC 1 cut(s) 401
SapI GCTCTTC 1 cut(s) 207
SaqAI TTAA 7 cut(s) 62, 119, 375, 465, 747, 995, 1102
SatI GCNGC 4 cut(s) 615, 879, 1043, 1050
Sau3AI GATC 2 cut(s) 154, 424
Sau96I GGNCC 3 cut(s) 79, 113, 964
SchI GAGTC 2 cut(s) 788, 1015
ScrFI CCNGG 2 cut(s) 74, 253
SduI GDGCHC 3 cut(s) 178, 401, 497
SfaNI GCATC 2 cut(s) 301, 862
SfcI CTRYAG 2 cut(s) 443, 459
SinI GGWCC 2 cut(s) 79, 964
Sse9I AATT 5 cut(s) 336, 482, 728, 816, 970
SsiI CCGC 2 cut(s) 57, 757
SspMI CTAG 3 cut(s) 477, 498, 531
SstI GAGCTC 1 cut(s) 401
StyD4I CCNGG 2 cut(s) 72, 251
TaiI ACGT 1 cut(s) 585
TaqI TCGA 1 cut(s) 395
TasI AATT 5 cut(s) 336, 482, 728, 816, 970
TfiI GAWTC 2 cut(s) 245, 392
Tru1I TTAA 7 cut(s) 62, 119, 375, 465, 747, 995, 1102
Tru9I TTAA 7 cut(s) 62, 119, 375, 465, 747, 995, 1102
TscAI CASTG 1 cut(s) 133
TseFI GTSAC 1 cut(s) 791
TseI GCWGC 4 cut(s) 614, 878, 1042, 1049
Tsp45I GTSAC 1 cut(s) 791
TspDTI ATGAA 2 cut(s) 17, 828
TspGWI ACGGA 2 cut(s) 168, 207
TspRI CASTG 1 cut(s) 133
VpaK11BI GGWCC 2 cut(s) 79, 964
XapI RAATTY 3 cut(s) 336, 482, 970
XspI CTAG 3 cut(s) 477, 498, 531
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.