Rw7G037650

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0008318
Physical Location & Seq
Reverse (-)
57095701 .. 57099105
3405 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G037650.1

Sequence Viewer

Length: 1560 bp
ATGAAGCTCTACAAAGACTACTACTTCCACCCCAACCACTCTCAGAGAGCCGCCTTGGCTGCCCTCTTCGTCCTTCTCTTCCCCACCTTCTTCCCCAATCTCTTCGGCCCATTGGGCCGCGCCTCTCCTTCCATCTTCTCTGAATGGAATGCTCCAAAGCCCAGACATTTACATCTTCTAGAAGCTGCCTTGTCAACCCAATCTTATGTTGAGCATCAGATCAAGGTTTGGGCTCCGCTGCCCAATCAGGGTTGGAAGCATTGCCCTGAAGAGCCTAAAAGTGCTTCATCGAAACCGAAAGGATCAAATGGGTATATACAGGTGTTCCTTGATGGAGGCTTGAATCAGCAGAAAATGGGGATTTGTGATGCGGTGGCTGTTGCTAAAATCTTGAATGCAACTCTGGTTCTACCGCACCTTGAAGTTAATCCCGTGTGGCAAGATTCGAGTTCCTTTGAGGAAATTTTTGATGTCGATCACTTTTTAGAAGTCCTACGCAATGAAGTTTCTATAGTTAAAGAACTTCCTAGTGAGTTTTCTTGGAGCTCTAGGGAGTATTATGCTACAGGCATACGGCCTACTAGAATTAAAACGGCACCTGTTCATGCTCCTGCTGAGTGGTATCTGGAAAATGTCTTGCCCATAATGCAGAGTTATGGAGTTGCTGCTATTTCCCCGTTCTCCCATCGTTTAACTTTCGACAATTTGCCCAAAAGCATTCAGCGCCTACGTTGTAAAGTGAACTTTCAAGCTTTAGCCTTTGTTCCTCATATTAGGGAATTGGGAGAAACCCTTGTTAATCGTCTCCGCAACCCTCCTGTAAGAATCCAAAACGCAGAAACTGACTCACAAGAGGGAACAAATGACCTTGATAAACAGGGAGCTGGGAAGTTTGTTGTTTGCATCTTCGCTTTGACAAAGTATGATATGGCTGCTCATTCCGCCTGTGATTTTGGTGGTGGTAAAGCTGAGAAACTTGCTCTTGCAAAATATCGACAAGTAATTTGGCAGGGAAGGGTCCTAAAATCTCAGTTCTCAGATGAGGAGCTGAGAGTTCAGGGGCGTTGCCCGTTGACGCCTGAAGAGATTGGATTGTTGCTAGCAGCTTTGGGCTTCAACAATAGCACCCGGCTTTATCTTGCTTCCCACAAGGTTTATGGTGGAGAAGCAAGGATCTCAACTCTGAGAAGATTATTTCCACTACTGGAGGATAAGAAGAGCCTTACCTCCGCCGAGAAAAGGGCTAAGGTTGAAGGCAAGGCTTCTCTGTTAGCTGCAGTTGACTATTATGTGAGCATGCAAAGTGACATCTTCATTTCTGCTTCTCCGGGAAATATGCACAATGCACTGGTGTGTCATCGAGCTTATATGAACTTGAAGACCATTAGACCGAGCATGACCCTGTTGAGTAAGCTGTTCTTGAATAAGAGCATGCAGTGGTCAGAATTCCAACATGCAGTTTTGGAAGGGCATAAAAACAGACAAGGGCAGATCAAGTTGAGAAAGGAGAAGCAATCCATATATACATATCCTGCTCCTGATTGCATGTGTCAAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

519

Amino Acids

58.51

Weight (kDa)

9.17

Isoelectric Point (pI)

45.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 105 - 457 1.4e-80 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 595
AccII CGCG 1 cut(s) 120
AciI CCGC 8 cut(s) 51, 118, 236, 371, 413, 808, 942, 1230
AclWI GGATC 2 cut(s) 310, 1181
AcsI RAATTY 2 cut(s) 462, 1445
AcuI CTGAAG 2 cut(s) 288, 1101
AcyI GRCGYC 1 cut(s) 1076
AfiI CCNNNNNNNGG 2 cut(s) 248, 1239
AgsI TTSAA 8 cut(s) 343, 394, 422, 749, 1117, 1253, 1378, 1423
AleI CACNNNNGTG 1 cut(s) 1351
Alw21I GWGCWC 1 cut(s) 548
Alw26I GTCTC 1 cut(s) 809
AlwI GGATC 2 cut(s) 310, 1181
AlwNI CAGNNNCTG 1 cut(s) 842
AoxI GGCC 3 cut(s) 106, 115, 575
ApeKI GCWGC 7 cut(s) 59, 185, 238, 665, 932, 1103, 1274
ApoI RAATTY 2 cut(s) 462, 1445
ArsI GACNNNNNNTTYG 2 cut(s) 987, 1019
AspLEI GCGC 2 cut(s) 122, 726
AspS9I GGNCC 3 cut(s) 107, 115, 1018
AsuC2I CCSGG 2 cut(s) 1129, 1329
AsuNHI GCTAGC 1 cut(s) 1099
AvaII GGWCC 1 cut(s) 1018
BanI GGYRCC 1 cut(s) 595
BanII GRGCYC 2 cut(s) 235, 548
BbsI GAAGAC 1 cut(s) 1385
Bbv12I GWGCWC 1 cut(s) 548
BbvI GCAGC 7 cut(s) 46, 172, 225, 652, 919, 1115, 1261
BccI CCATC 3 cut(s) 140, 326, 693
BceAI ACGGC 2 cut(s) 590, 609
BcnI CCSGG 2 cut(s) 1129, 1329
BcoDI GTCTC 1 cut(s) 809
BfaI CTAG 5 cut(s) 179, 528, 549, 582, 1100
BfmI CTRYAG 3 cut(s) 510, 564, 1275
BfoI RGCGCY 1 cut(s) 727
BglI GCCNNNNNGGC 2 cut(s) 56, 114
BisI GCNGC 9 cut(s) 51, 60, 118, 186, 239, 666, 933, 1104, 1275
BlsI GCNGC 9 cut(s) 52, 61, 119, 187, 240, 667, 934, 1105, 1276
Bme1390I CCNGG 2 cut(s) 1129, 1329
Bme18I GGWCC 1 cut(s) 1018
BmgT120I GGNCC 3 cut(s) 107, 115, 1018
BmiI GGNNCC 3 cut(s) 234, 597, 1019
BmrFI CCNGG 2 cut(s) 1129, 1329
BmsI GCATC 3 cut(s) 223, 358, 912
BmtI GCTAGC 1 cut(s) 1103
BpiI GAAGAC 1 cut(s) 1385
BpmI CTGGAG 1 cut(s) 1226
Bpu10I CCTNAGC 1 cut(s) 1245
BpuMI CCSGG 2 cut(s) 1129, 1329
BsaBI GATNNNNATC 1 cut(s) 474
BsaHI GRCGYC 1 cut(s) 1076
BsaJI CCNNGG 1 cut(s) 54
Bsc4I CCNNNNNNNGG 2 cut(s) 248, 1239
Bse1I ACTGG 2 cut(s) 1209, 1353
Bse3DI GCAATG 2 cut(s) 259, 505
Bse8I GATNNNNATC 1 cut(s) 474
BseDI CCNNGG 1 cut(s) 54
BseJI GATNNNNATC 1 cut(s) 474
BseLI CCNNNNNNNGG 2 cut(s) 248, 1239
BseMI GCAATG 2 cut(s) 259, 505
BseMII CTCAG 7 cut(s) 56, 606, 960, 1040, 1043, 1050, 1175
BseNI ACTGG 2 cut(s) 1209, 1353
BseRI GAGGAG 1 cut(s) 1058
BseXI GCAGC 7 cut(s) 46, 172, 225, 652, 919, 1115, 1261
BseYI CCCAGC 1 cut(s) 884
Bsh1236I CGCG 1 cut(s) 120
BshFI GGCC 3 cut(s) 108, 117, 577
BshNI GGYRCC 1 cut(s) 595
BsiHKAI GWGCWC 1 cut(s) 548
BsiSI CCGG 2 cut(s) 1129, 1328
BslI CCNNNNNNNGG 2 cut(s) 248, 1239
BsmAI GTCTC 1 cut(s) 809
BsmBI CGTCTC 1 cut(s) 809
BsmI GAATGC 3 cut(s) 154, 400, 717
BsnI GGCC 3 cut(s) 108, 117, 577
Bsp1286I GDGCHC 2 cut(s) 235, 548
Bsp143I GATC 5 cut(s) 219, 302, 475, 1173, 1491
BspACI CCGC 8 cut(s) 51, 118, 236, 371, 413, 808, 942, 1230
BspANI GGCC 3 cut(s) 108, 117, 577
BspCNI CTCAG 7 cut(s) 55, 607, 961, 1041, 1042, 1049, 1176
BspFNI CGCG 1 cut(s) 120
BspLI GGNNCC 3 cut(s) 234, 597, 1019
BspMAI CTGCAG 1 cut(s) 1279
BspOI GCTAGC 1 cut(s) 1103
BspPI GGATC 2 cut(s) 310, 1181
BspQI GCTCTTC 2 cut(s) 264, 1211
BspT107I GGYRCC 1 cut(s) 595
BsrDI GCAATG 2 cut(s) 259, 505
BsrI ACTGG 2 cut(s) 1209, 1353
BssECI CCNNGG 1 cut(s) 54
BssMI GATC 5 cut(s) 219, 302, 475, 1173, 1491
BssNI GRCGYC 1 cut(s) 1076
BssT1I CCWWGG 1 cut(s) 54
Bst6I CTCTTC 6 cut(s) 71, 83, 107, 264, 1077, 1211
BstACI GRCGYC 1 cut(s) 1076
BstC8I GCNNGC 3 cut(s) 1101, 1298, 1433
BstDEI CTNAG 8 cut(s) 42, 615, 969, 1029, 1036, 1049, 1184, 1245
BstFNI CGCG 1 cut(s) 120
BstH2I RGCGCY 1 cut(s) 727
BstHHI GCGC 2 cut(s) 122, 726
BstKTI GATC 5 cut(s) 222, 305, 478, 1176, 1494
BstMAI GTCTC 1 cut(s) 809
BstMBI GATC 5 cut(s) 219, 302, 475, 1173, 1491
BstMWI GCNNNNNNNGC 6 cut(s) 56, 59, 114, 646, 723, 941
BstNSI RCATGY 4 cut(s) 1300, 1435, 1457, 1549
BstSCI CCNGG 2 cut(s) 1127, 1327
BstSFI CTRYAG 3 cut(s) 510, 564, 1275
BstUI CGCG 1 cut(s) 120
BstV1I GCAGC 7 cut(s) 46, 172, 225, 652, 919, 1115, 1261
BstV2I GAAGAC 1 cut(s) 1385
BstX2I RGATCY 1 cut(s) 1173
BstYI RGATCY 1 cut(s) 1173
BsuRI GGCC 3 cut(s) 108, 117, 577
BtsI GCAGTG 1 cut(s) 1442
BtsIMutI CAGTG 2 cut(s) 1346, 1442
Cac8I GCNNGC 3 cut(s) 1101, 1298, 1433
CaiI CAGNNNCTG 1 cut(s) 842
CfoI GCGC 2 cut(s) 122, 726
Cfr13I GGNCC 3 cut(s) 107, 115, 1018
CseI GACGC 1 cut(s) 1084
CviAII CATG 6 cut(s) 605, 1297, 1396, 1432, 1454, 1546
DdeI CTNAG 8 cut(s) 42, 615, 969, 1029, 1036, 1049, 1184, 1245
DpnI GATC 5 cut(s) 221, 304, 477, 1175, 1493
DpnII GATC 5 cut(s) 219, 302, 475, 1173, 1491
Eam1104I CTCTTC 6 cut(s) 71, 83, 107, 264, 1077, 1211
EarI CTCTTC 6 cut(s) 71, 83, 107, 264, 1077, 1211
EciI GGCGGA 2 cut(s) 931, 1219
Ecl136II GAGCTC 1 cut(s) 546
Eco130I CCWWGG 1 cut(s) 54
Eco24I GRGCYC 2 cut(s) 235, 548
Eco47I GGWCC 1 cut(s) 1018
Eco53kI GAGCTC 1 cut(s) 546
Eco57I CTGAAG 2 cut(s) 288, 1101
EcoICRI GAGCTC 1 cut(s) 546
EcoO109I RGGNCCY 1 cut(s) 1018
EcoRI GAATTC 1 cut(s) 1445
EcoT14I CCWWGG 1 cut(s) 54
EcoT38I GRGCYC 2 cut(s) 235, 548
ErhI CCWWGG 1 cut(s) 54
Esp3I CGTCTC 1 cut(s) 809
FaeI CATG 6 cut(s) 608, 1300, 1399, 1435, 1457, 1549
FalI AAGNNNNNCTT 2 cut(s) 1403, 1435
FatI CATG 6 cut(s) 604, 1296, 1395, 1431, 1453, 1545
Fnu4HI GCNGC 9 cut(s) 51, 60, 118, 186, 239, 666, 933, 1104, 1275
FriOI GRGCYC 2 cut(s) 235, 548
Fsp4HI GCNGC 9 cut(s) 51, 60, 118, 186, 239, 666, 933, 1104, 1275
FspBI CTAG 5 cut(s) 179, 528, 549, 582, 1100
GlaI GCGC 2 cut(s) 121, 725
GluI GCNGC 9 cut(s) 51, 60, 118, 186, 239, 666, 933, 1104, 1275
GsaI CCCAGC 1 cut(s) 888
GsuI CTGGAG 1 cut(s) 1226
HaeII RGCGCY 1 cut(s) 727
HaeIII GGCC 3 cut(s) 108, 117, 577
HapII CCGG 2 cut(s) 1129, 1328
HgaI GACGC 1 cut(s) 1084
HhaI GCGC 2 cut(s) 122, 726
Hin1I GRCGYC 1 cut(s) 1076
Hin1II CATG 6 cut(s) 608, 1300, 1399, 1435, 1457, 1549
Hin6I GCGC 2 cut(s) 120, 724
HinP1I GCGC 2 cut(s) 120, 724
HincII GTYRAC 3 cut(s) 195, 1074, 1282
HindII GTYRAC 3 cut(s) 195, 1074, 1282
HindIII AAGCTT 2 cut(s) 750, 1554
HinfI GANTC 4 cut(s) 343, 443, 825, 845
HpaII CCGG 2 cut(s) 1129, 1328
Hpy166II GTNNAC 4 cut(s) 195, 742, 1074, 1282
Hpy188I TCNGA 6 cut(s) 45, 142, 219, 1039, 1185, 1444
Hpy188III TCNNGA 5 cut(s) 179, 391, 626, 1420, 1538
Hpy8I GTNNAC 4 cut(s) 195, 742, 1074, 1282
HpyAV CCTTC 6 cut(s) 83, 97, 138, 1008, 1247, 1460
HpyCH4IV ACGT 1 cut(s) 730
HpyF10VI GCNNNNNNNGC 6 cut(s) 56, 59, 114, 646, 723, 941
HpyF3I CTNAG 8 cut(s) 42, 615, 969, 1029, 1036, 1049, 1184, 1245
HpySE526I ACGT 1 cut(s) 730
Hsp92I GRCGYC 1 cut(s) 1076
Hsp92II CATG 6 cut(s) 608, 1300, 1399, 1435, 1457, 1549
HspAI GCGC 2 cut(s) 120, 724
Kzo9I GATC 5 cut(s) 219, 302, 475, 1173, 1491
LguI GCTCTTC 2 cut(s) 264, 1211
LmnI GCTCC 7 cut(s) 157, 238, 543, 613, 881, 1045, 1540
Lsp1109I GCAGC 7 cut(s) 46, 172, 225, 652, 919, 1115, 1261
LweI GCATC 3 cut(s) 223, 358, 912
MaeI CTAG 5 cut(s) 179, 528, 549, 582, 1100
MaeII ACGT 1 cut(s) 730
MaeIII GTNAC 1 cut(s) 1304
MalI GATC 5 cut(s) 221, 304, 477, 1175, 1493
MboI GATC 5 cut(s) 219, 302, 475, 1173, 1491
MflI RGATCY 1 cut(s) 1173
MhlI GDGCHC 2 cut(s) 235, 548
MluCI AATT 6 cut(s) 462, 585, 703, 779, 1002, 1445
MlyI GAGTC 1 cut(s) 839
MmeI TCCRAC 2 cut(s) 233, 1474
MseI TTAA 6 cut(s) 426, 516, 588, 692, 798, 1558
MslI CAYNNNNRTG 1 cut(s) 1351
MspA1I CMGCKG 1 cut(s) 238
MspI CCGG 2 cut(s) 1129, 1328
MspR9I CCNGG 2 cut(s) 1129, 1329
Mva1269I GAATGC 3 cut(s) 154, 400, 717
MvnI CGCG 1 cut(s) 120
MwoI GCNNNNNNNGC 6 cut(s) 56, 59, 114, 646, 723, 941
NciI CCSGG 2 cut(s) 1129, 1329
NdeII GATC 5 cut(s) 219, 302, 475, 1173, 1491
NheI GCTAGC 1 cut(s) 1099
NlaIII CATG 6 cut(s) 608, 1300, 1399, 1435, 1457, 1549
NlaIV GGNNCC 3 cut(s) 234, 597, 1019
NmeAIII GCCGAG 1 cut(s) 1258
NmuCI GTSAC 1 cut(s) 1304
NspI RCATGY 4 cut(s) 1300, 1435, 1457, 1549
OliI CACNNNNGTG 1 cut(s) 1351
PaeI GCATGC 2 cut(s) 1300, 1435
PciSI GCTCTTC 2 cut(s) 264, 1211
PctI GAATGC 3 cut(s) 154, 400, 717
PfeI GAWTC 3 cut(s) 343, 443, 825
PfoI TCCNGGA 1 cut(s) 1327
PkrI GCNGC 9 cut(s) 52, 61, 119, 187, 240, 667, 934, 1105, 1276
PleI GAGTC 1 cut(s) 839
PpsI GAGTC 1 cut(s) 839
PpuMI RGGWCCY 1 cut(s) 1018
Psp124BI GAGCTC 1 cut(s) 548
Psp5II RGGWCCY 1 cut(s) 1018
PspFI CCCAGC 1 cut(s) 884
PspN4I GGNNCC 3 cut(s) 234, 597, 1019
PspPI GGNCC 3 cut(s) 107, 115, 1018
PspPPI RGGWCCY 1 cut(s) 1018
PstI CTGCAG 1 cut(s) 1279
PstNI CAGNNNCTG 1 cut(s) 842
PsuI RGATCY 1 cut(s) 1173
RseI CAYNNNNRTG 1 cut(s) 1351
SacI GAGCTC 1 cut(s) 548
SapI GCTCTTC 2 cut(s) 264, 1211
SaqAI TTAA 6 cut(s) 426, 516, 588, 692, 798, 1558
SatI GCNGC 9 cut(s) 51, 60, 118, 186, 239, 666, 933, 1104, 1275
Sau3AI GATC 5 cut(s) 219, 302, 475, 1173, 1491
Sau96I GGNCC 3 cut(s) 107, 115, 1018
SchI GAGTC 1 cut(s) 839
ScrFI CCNGG 2 cut(s) 1129, 1329
SduI GDGCHC 2 cut(s) 235, 548
SfaNI GCATC 3 cut(s) 223, 358, 912
SfcI CTRYAG 3 cut(s) 510, 564, 1275
SfiI GGCCNNNNNGGCC 1 cut(s) 114
SinI GGWCC 1 cut(s) 1018
SmiMI CAYNNNNRTG 1 cut(s) 1351
SphI GCATGC 2 cut(s) 1300, 1435
Sse9I AATT 6 cut(s) 462, 585, 703, 779, 1002, 1445
SsiI CCGC 8 cut(s) 51, 118, 236, 371, 413, 808, 942, 1230
SspMI CTAG 5 cut(s) 179, 528, 549, 582, 1100
SstI GAGCTC 1 cut(s) 548
StyD4I CCNGG 2 cut(s) 1127, 1327
StyI CCWWGG 1 cut(s) 54
TaiI ACGT 1 cut(s) 733
TaqI TCGA 6 cut(s) 290, 446, 474, 699, 994, 1360
TaqII GACCGA 1 cut(s) 1405
TasI AATT 6 cut(s) 462, 585, 703, 779, 1002, 1445
TauI GCSGC 2 cut(s) 53, 120
TfiI GAWTC 3 cut(s) 343, 443, 825
Tru1I TTAA 6 cut(s) 426, 516, 588, 692, 798, 1558
Tru9I TTAA 6 cut(s) 426, 516, 588, 692, 798, 1558
TscAI CASTG 2 cut(s) 1353, 1442
TseFI GTSAC 1 cut(s) 1304
TseI GCWGC 7 cut(s) 59, 185, 238, 665, 932, 1103, 1274
Tsp45I GTSAC 1 cut(s) 1304
TspDTI ATGAA 6 cut(s) 17, 276, 516, 593, 1303, 1385
TspRI CASTG 2 cut(s) 1353, 1442
VpaK11BI GGWCC 1 cut(s) 1018
XapI RAATTY 2 cut(s) 462, 1445
XbaI TCTAGA 1 cut(s) 178
XceI RCATGY 4 cut(s) 1300, 1435, 1457, 1549
XcmI CCANNNNNNNNNTGG 1 cut(s) 1154
XspI CTAG 5 cut(s) 179, 528, 549, 582, 1100
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.