Rh7AG454100

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0008318
Physical Location & Seq
Reverse (-)
62307661 .. 62311449
3789 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG454100.1

Sequence Viewer

Length: 1542 bp
ATGAAGCTCTACAAAGACTACTACTTCCACCCCAACCACTCTCAGAGAGCCGCCTTGGCTGCCCTCTTCGTCCTTCTCTTCCCCACCTTCTTCCCCAATCTCTTCGGCCCATTGGGCCGCGCCTCTCCTTCCATCTTCTCTGAATGGAATGCTCCAAAGCCCAGACATTTACATCTTCTAGAAGCAGCCTTGTCAACCCAATCTATCAAGGTTTGGGCTCCGCTGCCCAATCAGGGATGGAAGCATTGCCCTGAAGAGCCTAAAAGTGCTTCATCGAAACCGAAAGGATCAAATGGGTATATACAGGTGTTCCTTGATGGAGGCTTGAACCAGCAGAAAATGGGGATTTGTGATGCGGTGGCTGTTGCTAAAATCTTGAATGCAACTCTGGTTCTACCGCACCTTGAAGTTAATCCCGTGTGGCAAGATTCGAGTTCCTTTGAGGAAATTTTTGATGTCGATCACTTTTTAGAAGTCCTACGCAATGAAGTTTCTATAGTTAAAGAACTTCCTAGTGAGTTTTCTTGGAGCTCTAGGGAGTATTATGCTACAGGCATACGGCCTACTAGAATTAAAACGGCACCTGTTCATGCTCCTGCTGAGTGGTATCTGGAAAATGTCTTGCCCATAATGCAGAGTTATGGAGTTGCTGCTATTTCCCCGTTCTCCCATCGTTTAACTTTCGACAATTTGCCCGAAAGCATTCAGCGCCTACGTTGTAAAGTGAACTTTCAAGCTTTAGCCTTTGTTCCTCATATTAGGGAATTGGGAGAAACCCTTGTTAATCGTCTCCGCAACCCTCCTGTAAGAATCCAAAACGCAGAAACTGACTCACAAGAGGGAACAAATGACCTTGATAAACAGGGAGCTGGGAAGTTTGTTGTTTTGCATCTTCGCTTTGACAAAGATATGGCTGCTCATTCCGCCTGTGATTTTGGTGGTGGTAAAGCTGAGAAACTTGCTCTTGCAAAATATCGACAAGTAATTTGGCAGGGAAGGGTCCTAAAATCTCAGTTCTCAGATGAGGAGCTGAGAGGTCAGGGGCGTTGCCCGTTGACGCCTGAAGAGATTGGATTGTTGCTAGCAGCTCTGGGCTTCAACAATAGCACCCGGCTTTATCTTGCTTCCCACAAGGTTTATGGTGGAGAAGCAAGGATCTCAACTCTGAGAAGATTATTTCCACTACTGGAGGATAAGAAGAGCCTTACCTCCGCCGAGAAAAGGGCTAAGGTTGAAGGCAAGGCTTCTCTGTTAGCTGCAGTTGACTATTATGTGAGCATGCAAAGTGACATCTTCATTTCTGCTTCTCCGGGAAATATGCACAATGCACTGGTGTGTCATCGAGCTTATATGAACTTGAAGACTATTAGACCAAGCATGACCCTGTTGAGTAAGCTGTTCTTGAATAAGAGCATGCAGTGGTCAGAATTCCAACATGCAGTTTTGGAAGGGCATAAAAACAGACAAGGGCAGATCAAGTTGAGAAAGGAGAAGCAATCCATATATACATATCCTGCTCCTGATTGCATGTGTCAAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

513

Amino Acids

57.78

Weight (kDa)

9.16

Isoelectric Point (pI)

46.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 100 - 451 1.2e-88 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 580
AccII CGCG 1 cut(s) 120
AciI CCGC 8 cut(s) 51, 118, 221, 356, 398, 793, 924, 1212
AclWI GGATC 2 cut(s) 295, 1163
AcsI RAATTY 2 cut(s) 447, 1427
AcuI CTGAAG 2 cut(s) 273, 1083
AcyI GRCGYC 1 cut(s) 1058
AfiI CCNNNNNNNGG 2 cut(s) 233, 1221
AgsI TTSAA 8 cut(s) 328, 379, 407, 734, 1099, 1235, 1360, 1405
AleI CACNNNNGTG 1 cut(s) 1333
Alw21I GWGCWC 1 cut(s) 533
Alw26I GTCTC 1 cut(s) 794
AlwI GGATC 2 cut(s) 295, 1163
AlwNI CAGNNNCTG 1 cut(s) 827
AoxI GGCC 3 cut(s) 106, 115, 560
ApeKI GCWGC 7 cut(s) 59, 185, 223, 650, 914, 1085, 1256
ApoI RAATTY 2 cut(s) 447, 1427
ArsI GACNNNNNNTTYG 2 cut(s) 969, 1001
Asp700I GAANNNNTTC 1 cut(s) 702
AspLEI GCGC 2 cut(s) 122, 711
AspS9I GGNCC 3 cut(s) 107, 115, 1000
AsuC2I CCSGG 2 cut(s) 1111, 1311
AsuNHI GCTAGC 1 cut(s) 1081
AvaII GGWCC 1 cut(s) 1000
BanI GGYRCC 1 cut(s) 580
BanII GRGCYC 2 cut(s) 220, 533
BbsI GAAGAC 1 cut(s) 1367
Bbv12I GWGCWC 1 cut(s) 533
BbvI GCAGC 7 cut(s) 46, 197, 210, 637, 901, 1097, 1243
BccI CCATC 4 cut(s) 140, 231, 311, 678
BceAI ACGGC 2 cut(s) 575, 594
BcnI CCSGG 2 cut(s) 1111, 1311
BcoDI GTCTC 1 cut(s) 794
BfaI CTAG 5 cut(s) 179, 513, 534, 567, 1082
BfmI CTRYAG 3 cut(s) 495, 549, 1257
BfoI RGCGCY 1 cut(s) 712
BglI GCCNNNNNGGC 2 cut(s) 56, 114
BisI GCNGC 9 cut(s) 51, 60, 118, 186, 224, 651, 915, 1086, 1257
BlsI GCNGC 9 cut(s) 52, 61, 119, 187, 225, 652, 916, 1087, 1258
Bme1390I CCNGG 2 cut(s) 1111, 1311
Bme18I GGWCC 1 cut(s) 1000
BmgT120I GGNCC 3 cut(s) 107, 115, 1000
BmiI GGNNCC 3 cut(s) 219, 582, 1001
BmrFI CCNGG 2 cut(s) 1111, 1311
BmsI GCATC 2 cut(s) 343, 898
BmtI GCTAGC 1 cut(s) 1085
BpiI GAAGAC 1 cut(s) 1367
BpmI CTGGAG 1 cut(s) 1208
Bpu10I CCTNAGC 1 cut(s) 1227
BpuMI CCSGG 2 cut(s) 1111, 1311
BsaBI GATNNNNATC 1 cut(s) 459
BsaHI GRCGYC 1 cut(s) 1058
BsaJI CCNNGG 1 cut(s) 54
Bsc4I CCNNNNNNNGG 2 cut(s) 233, 1221
Bse1I ACTGG 2 cut(s) 1191, 1335
Bse3DI GCAATG 2 cut(s) 244, 490
Bse8I GATNNNNATC 1 cut(s) 459
BseDI CCNNGG 1 cut(s) 54
BseGI GGATG 1 cut(s) 242
BseJI GATNNNNATC 1 cut(s) 459
BseLI CCNNNNNNNGG 2 cut(s) 233, 1221
BseMI GCAATG 2 cut(s) 244, 490
BseMII CTCAG 7 cut(s) 56, 591, 942, 1022, 1025, 1032, 1157
BseNI ACTGG 2 cut(s) 1191, 1335
BseRI GAGGAG 1 cut(s) 1040
BseXI GCAGC 7 cut(s) 46, 197, 210, 637, 901, 1097, 1243
BseYI CCCAGC 1 cut(s) 869
Bsh1236I CGCG 1 cut(s) 120
BshFI GGCC 3 cut(s) 108, 117, 562
BshNI GGYRCC 1 cut(s) 580
BsiHKAI GWGCWC 1 cut(s) 533
BsiSI CCGG 2 cut(s) 1111, 1310
BslI CCNNNNNNNGG 2 cut(s) 233, 1221
BsmAI GTCTC 1 cut(s) 794
BsmBI CGTCTC 1 cut(s) 794
BsmI GAATGC 3 cut(s) 154, 385, 702
BsnI GGCC 3 cut(s) 108, 117, 562
Bsp1286I GDGCHC 2 cut(s) 220, 533
Bsp143I GATC 4 cut(s) 287, 460, 1155, 1473
BspACI CCGC 8 cut(s) 51, 118, 221, 356, 398, 793, 924, 1212
BspANI GGCC 3 cut(s) 108, 117, 562
BspCNI CTCAG 7 cut(s) 55, 592, 943, 1023, 1024, 1031, 1158
BspFNI CGCG 1 cut(s) 120
BspLI GGNNCC 3 cut(s) 219, 582, 1001
BspMAI CTGCAG 1 cut(s) 1261
BspOI GCTAGC 1 cut(s) 1085
BspPI GGATC 2 cut(s) 295, 1163
BspQI GCTCTTC 2 cut(s) 249, 1193
BspT107I GGYRCC 1 cut(s) 580
BsrDI GCAATG 2 cut(s) 244, 490
BsrI ACTGG 2 cut(s) 1191, 1335
BssECI CCNNGG 1 cut(s) 54
BssMI GATC 4 cut(s) 287, 460, 1155, 1473
BssNI GRCGYC 1 cut(s) 1058
BssT1I CCWWGG 1 cut(s) 54
Bst6I CTCTTC 6 cut(s) 71, 83, 107, 249, 1059, 1193
BstACI GRCGYC 1 cut(s) 1058
BstC8I GCNNGC 3 cut(s) 1083, 1280, 1415
BstDEI CTNAG 8 cut(s) 42, 600, 951, 1011, 1018, 1031, 1166, 1227
BstF5I GGATG 1 cut(s) 242
BstFNI CGCG 1 cut(s) 120
BstH2I RGCGCY 1 cut(s) 712
BstHHI GCGC 2 cut(s) 122, 711
BstKTI GATC 4 cut(s) 290, 463, 1158, 1476
BstMAI GTCTC 1 cut(s) 794
BstMBI GATC 4 cut(s) 287, 460, 1155, 1473
BstMWI GCNNNNNNNGC 6 cut(s) 56, 59, 114, 631, 708, 923
BstNSI RCATGY 4 cut(s) 1282, 1417, 1439, 1531
BstSCI CCNGG 2 cut(s) 1109, 1309
BstSFI CTRYAG 3 cut(s) 495, 549, 1257
BstUI CGCG 1 cut(s) 120
BstV1I GCAGC 7 cut(s) 46, 197, 210, 637, 901, 1097, 1243
BstV2I GAAGAC 1 cut(s) 1367
BstX2I RGATCY 1 cut(s) 1155
BstYI RGATCY 1 cut(s) 1155
BsuRI GGCC 3 cut(s) 108, 117, 562
BtsCI GGATG 1 cut(s) 242
BtsI GCAGTG 1 cut(s) 1424
BtsIMutI CAGTG 2 cut(s) 1328, 1424
Cac8I GCNNGC 3 cut(s) 1083, 1280, 1415
CaiI CAGNNNCTG 1 cut(s) 827
CfoI GCGC 2 cut(s) 122, 711
Cfr13I GGNCC 3 cut(s) 107, 115, 1000
CseI GACGC 1 cut(s) 1066
CviAII CATG 6 cut(s) 590, 1279, 1378, 1414, 1436, 1528
DdeI CTNAG 8 cut(s) 42, 600, 951, 1011, 1018, 1031, 1166, 1227
DpnI GATC 4 cut(s) 289, 462, 1157, 1475
DpnII GATC 4 cut(s) 287, 460, 1155, 1473
Eam1104I CTCTTC 6 cut(s) 71, 83, 107, 249, 1059, 1193
EarI CTCTTC 6 cut(s) 71, 83, 107, 249, 1059, 1193
EciI GGCGGA 2 cut(s) 913, 1201
Ecl136II GAGCTC 1 cut(s) 531
Eco130I CCWWGG 1 cut(s) 54
Eco24I GRGCYC 2 cut(s) 220, 533
Eco47I GGWCC 1 cut(s) 1000
Eco53kI GAGCTC 1 cut(s) 531
Eco57I CTGAAG 2 cut(s) 273, 1083
EcoICRI GAGCTC 1 cut(s) 531
EcoO109I RGGNCCY 1 cut(s) 1000
EcoRI GAATTC 1 cut(s) 1427
EcoT14I CCWWGG 1 cut(s) 54
EcoT38I GRGCYC 2 cut(s) 220, 533
ErhI CCWWGG 1 cut(s) 54
Esp3I CGTCTC 1 cut(s) 794
FaeI CATG 6 cut(s) 593, 1282, 1381, 1417, 1439, 1531
FalI AAGNNNNNCTT 2 cut(s) 1385, 1417
FatI CATG 6 cut(s) 589, 1278, 1377, 1413, 1435, 1527
Fnu4HI GCNGC 9 cut(s) 51, 60, 118, 186, 224, 651, 915, 1086, 1257
FokI GGATG 1 cut(s) 249
FriOI GRGCYC 2 cut(s) 220, 533
Fsp4HI GCNGC 9 cut(s) 51, 60, 118, 186, 224, 651, 915, 1086, 1257
FspBI CTAG 5 cut(s) 179, 513, 534, 567, 1082
GlaI GCGC 2 cut(s) 121, 710
GluI GCNGC 9 cut(s) 51, 60, 118, 186, 224, 651, 915, 1086, 1257
GsaI CCCAGC 1 cut(s) 873
GsuI CTGGAG 1 cut(s) 1208
HaeII RGCGCY 1 cut(s) 712
HaeIII GGCC 3 cut(s) 108, 117, 562
HapII CCGG 2 cut(s) 1111, 1310
HgaI GACGC 1 cut(s) 1066
HhaI GCGC 2 cut(s) 122, 711
Hin1I GRCGYC 1 cut(s) 1058
Hin1II CATG 6 cut(s) 593, 1282, 1381, 1417, 1439, 1531
Hin6I GCGC 2 cut(s) 120, 709
HinP1I GCGC 2 cut(s) 120, 709
HincII GTYRAC 3 cut(s) 195, 1056, 1264
HindII GTYRAC 3 cut(s) 195, 1056, 1264
HindIII AAGCTT 2 cut(s) 735, 1536
HinfI GANTC 3 cut(s) 428, 810, 830
HpaII CCGG 2 cut(s) 1111, 1310
Hpy166II GTNNAC 4 cut(s) 195, 727, 1056, 1264
Hpy188I TCNGA 5 cut(s) 45, 142, 1021, 1167, 1426
Hpy188III TCNNGA 5 cut(s) 179, 376, 611, 1402, 1520
Hpy8I GTNNAC 4 cut(s) 195, 727, 1056, 1264
HpyAV CCTTC 6 cut(s) 83, 97, 138, 990, 1229, 1442
HpyCH4IV ACGT 1 cut(s) 715
HpyF10VI GCNNNNNNNGC 6 cut(s) 56, 59, 114, 631, 708, 923
HpyF3I CTNAG 8 cut(s) 42, 600, 951, 1011, 1018, 1031, 1166, 1227
HpySE526I ACGT 1 cut(s) 715
Hsp92I GRCGYC 1 cut(s) 1058
Hsp92II CATG 6 cut(s) 593, 1282, 1381, 1417, 1439, 1531
HspAI GCGC 2 cut(s) 120, 709
Kzo9I GATC 4 cut(s) 287, 460, 1155, 1473
LguI GCTCTTC 2 cut(s) 249, 1193
LmnI GCTCC 7 cut(s) 157, 223, 528, 598, 866, 1027, 1522
Lsp1109I GCAGC 7 cut(s) 46, 197, 210, 637, 901, 1097, 1243
LweI GCATC 2 cut(s) 343, 898
MaeI CTAG 5 cut(s) 179, 513, 534, 567, 1082
MaeII ACGT 1 cut(s) 715
MaeIII GTNAC 1 cut(s) 1286
MalI GATC 4 cut(s) 289, 462, 1157, 1475
MboI GATC 4 cut(s) 287, 460, 1155, 1473
MflI RGATCY 1 cut(s) 1155
MhlI GDGCHC 2 cut(s) 220, 533
MluCI AATT 6 cut(s) 447, 570, 688, 764, 984, 1427
MlyI GAGTC 1 cut(s) 824
MmeI TCCRAC 1 cut(s) 1456
MroXI GAANNNNTTC 1 cut(s) 702
MseI TTAA 6 cut(s) 411, 501, 573, 677, 783, 1540
MslI CAYNNNNRTG 1 cut(s) 1333
MspA1I CMGCKG 1 cut(s) 223
MspI CCGG 2 cut(s) 1111, 1310
MspR9I CCNGG 2 cut(s) 1111, 1311
Mva1269I GAATGC 3 cut(s) 154, 385, 702
MvnI CGCG 1 cut(s) 120
MwoI GCNNNNNNNGC 6 cut(s) 56, 59, 114, 631, 708, 923
NciI CCSGG 2 cut(s) 1111, 1311
NdeII GATC 4 cut(s) 287, 460, 1155, 1473
NheI GCTAGC 1 cut(s) 1081
NlaIII CATG 6 cut(s) 593, 1282, 1381, 1417, 1439, 1531
NlaIV GGNNCC 3 cut(s) 219, 582, 1001
NmeAIII GCCGAG 1 cut(s) 1240
NmuCI GTSAC 1 cut(s) 1286
NspI RCATGY 4 cut(s) 1282, 1417, 1439, 1531
OliI CACNNNNGTG 1 cut(s) 1333
PaeI GCATGC 2 cut(s) 1282, 1417
PciSI GCTCTTC 2 cut(s) 249, 1193
PctI GAATGC 3 cut(s) 154, 385, 702
PdmI GAANNNNTTC 1 cut(s) 702
PfeI GAWTC 2 cut(s) 428, 810
PfoI TCCNGGA 1 cut(s) 1309
PkrI GCNGC 9 cut(s) 52, 61, 119, 187, 225, 652, 916, 1087, 1258
PleI GAGTC 1 cut(s) 824
PpsI GAGTC 1 cut(s) 824
PpuMI RGGWCCY 1 cut(s) 1000
Psp124BI GAGCTC 1 cut(s) 533
Psp5II RGGWCCY 1 cut(s) 1000
PspFI CCCAGC 1 cut(s) 869
PspN4I GGNNCC 3 cut(s) 219, 582, 1001
PspPI GGNCC 3 cut(s) 107, 115, 1000
PspPPI RGGWCCY 1 cut(s) 1000
PstI CTGCAG 1 cut(s) 1261
PstNI CAGNNNCTG 1 cut(s) 827
PsuI RGATCY 1 cut(s) 1155
RseI CAYNNNNRTG 1 cut(s) 1333
SacI GAGCTC 1 cut(s) 533
SapI GCTCTTC 2 cut(s) 249, 1193
SaqAI TTAA 6 cut(s) 411, 501, 573, 677, 783, 1540
SatI GCNGC 9 cut(s) 51, 60, 118, 186, 224, 651, 915, 1086, 1257
Sau3AI GATC 4 cut(s) 287, 460, 1155, 1473
Sau96I GGNCC 3 cut(s) 107, 115, 1000
SchI GAGTC 1 cut(s) 824
ScrFI CCNGG 2 cut(s) 1111, 1311
SduI GDGCHC 2 cut(s) 220, 533
SfaNI GCATC 2 cut(s) 343, 898
SfcI CTRYAG 3 cut(s) 495, 549, 1257
SfiI GGCCNNNNNGGCC 1 cut(s) 114
SinI GGWCC 1 cut(s) 1000
SmiMI CAYNNNNRTG 1 cut(s) 1333
SphI GCATGC 2 cut(s) 1282, 1417
Sse9I AATT 6 cut(s) 447, 570, 688, 764, 984, 1427
SsiI CCGC 8 cut(s) 51, 118, 221, 356, 398, 793, 924, 1212
SspMI CTAG 5 cut(s) 179, 513, 534, 567, 1082
SstI GAGCTC 1 cut(s) 533
StyD4I CCNGG 2 cut(s) 1109, 1309
StyI CCWWGG 1 cut(s) 54
TaiI ACGT 1 cut(s) 718
TaqI TCGA 6 cut(s) 275, 431, 459, 684, 976, 1342
TasI AATT 6 cut(s) 447, 570, 688, 764, 984, 1427
TauI GCSGC 2 cut(s) 53, 120
TfiI GAWTC 2 cut(s) 428, 810
Tru1I TTAA 6 cut(s) 411, 501, 573, 677, 783, 1540
Tru9I TTAA 6 cut(s) 411, 501, 573, 677, 783, 1540
TscAI CASTG 2 cut(s) 1335, 1424
TseFI GTSAC 1 cut(s) 1286
TseI GCWGC 7 cut(s) 59, 185, 223, 650, 914, 1085, 1256
Tsp45I GTSAC 1 cut(s) 1286
TspDTI ATGAA 6 cut(s) 17, 261, 501, 578, 1285, 1367
TspRI CASTG 2 cut(s) 1335, 1424
VpaK11BI GGWCC 1 cut(s) 1000
XapI RAATTY 2 cut(s) 447, 1427
XbaI TCTAGA 1 cut(s) 178
XceI RCATGY 4 cut(s) 1282, 1417, 1439, 1531
XcmI CCANNNNNNNNNTGG 1 cut(s) 1136
XmnI GAANNNNTTC 1 cut(s) 702
XspI CTAG 5 cut(s) 179, 513, 534, 567, 1082
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.