pycom08g16980

At1g04910-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0008318
Physical Location & Seq
Forward (+)
17190335 .. 17193321
2987 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g16980.1

Sequence Viewer

Length: 1191 bp
ATGAAGCTTCACAAGGACTACTATGGCTACTGCTACCCCAACCAGTCTCAGAAAGCCGCTTTAGCTGGCCTCCTGGTGGTCCTCTTTCCGGCCTTCCTGCCAAACCTGTTTGGCCCATTAAGCCACGCTGCTCCTTCCATCTTCTCGGAGTGGAATGCTCCGAAACCCCGGCACTTGCACCTTCTAAATCCGGCTTTACTTCTTCAATCTTATGTGGATCAACAGAGCAAGCTTTGGGCTCCGTTGCCCAACCAAGGATGGAAGCATTGCCCTGAAGAGCCTAATAGTTTATCATCATCGCCAAAAGAATCTACCTGGTATATTCAGGTGTTCCTTGACGGAGGCTTGAACCAGCAGAAAATGGGGATTTGTGATGCAGTTGCTGTTGCTAAAATTTTGAATGCCACTTTGGTTATTCCACACCTTGAAGTTAATCCCGTGTGGCAAGATTCGAGCTCCTTTGAGGACATTTTTGATGTGGATCACTTTATTGAAGCCCTACAGGGTGAGGTTTCTATAGTTAAAGCCCTTCCTAGTGAATTTTCTTGGAGCACTAGGGAGTATTATGCTACTGGCATAAGGATTACTAGAATAAAAACAGCACCTGTTCACGCTTCTTCTGATTGGTATCTGGAAAACGTCTTGCCCATATTGCAGAGATATGGAGTTGCTGCTATCTCCCCATTTTCTCATCGTTTGGCCTTCGACAACTTGCCTCAAAACATACAGCACTTACGTTGTAAAGTCAACTTTCAAGCTTTAGCCTTTGTTCCTCATATCAGGGAATTGGGAGAAACCCTTGTTAATCGTCTCCGCTACCCTCCCAATAGAAACCCAAAAGCAGGTAGTGACTCACAGGACAAGGCAAATGAAATTGAGAAACAGGGAGCTGGGAAATATGTTGTTTTGCATCTTCGCTTCGATAAGGACATGGCTGCTCATTCAGCCTGTGATTTTGGTGGAGGTAAAGCTGAGAAACTCGCCCTTGCAAAATATCGGCAAGTAATCTGGCAGGGTAGGGTCCTAAAATCTCAGTTCACAGATGAGGATTTAAGAAATCAGGGGCGTTGCCCATTGACTCCTGAAGAGATTGGATTGCTGCTAGCAGCTTTGGGCTTCAACAATACCACCCGACTCTATCTTGCTTCTTACAAGGTTTACGGCGGAGAAGCAAGGATCGATCGACTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

397

Amino Acids

44.55

Weight (kDa)

8.39

Isoelectric Point (pI)

49.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 107 - 396 5e-72 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 833
AciI CCGC 3 cut(s) 57, 814, 1164
AclWI GGATC 3 cut(s) 225, 489, 1184
AcsI RAATTY 2 cut(s) 393, 539
AcuI CTGAAG 2 cut(s) 294, 1104
AfiI CCNNNNNNNGG 4 cut(s) 76, 88, 254, 842
AgsI TTSAA 7 cut(s) 206, 349, 400, 428, 494, 755, 1120
AjnI CCWGG 2 cut(s) 72, 314
AjuI GAANNNNNNNTTGG 2 cut(s) 392, 424
AluBI AGCT 8 cut(s) 7, 65, 232, 456, 758, 890, 971, 1109
AluI AGCT 8 cut(s) 7, 65, 232, 456, 758, 890, 971, 1109
Alw21I GWGCWC 2 cut(s) 458, 554
Alw26I GTCTC 2 cut(s) 51, 815
AlwI GGATC 3 cut(s) 225, 489, 1184
AlwNI CAGNNNCTG 2 cut(s) 383, 605
AoxI GGCC 4 cut(s) 67, 90, 112, 699
ApeKI GCWGC 5 cut(s) 128, 671, 935, 1099, 1106
ApoI RAATTY 2 cut(s) 393, 539
AspS9I GGNCC 3 cut(s) 79, 113, 1021
AsuC2I CCSGG 1 cut(s) 169
AsuHPI GGTGA 1 cut(s) 518
AsuNHI GCTAGC 1 cut(s) 1102
AvaII GGWCC 2 cut(s) 79, 1021
BanII GRGCYC 2 cut(s) 241, 458
Bbv12I GWGCWC 2 cut(s) 458, 554
BbvI GCAGC 5 cut(s) 115, 658, 922, 1086, 1118
BccI CCATC 2 cut(s) 146, 252
BceAI ACGGC 1 cut(s) 1177
BciT130I CCWGG 2 cut(s) 74, 316
BcnI CCSGG 1 cut(s) 169
BcoDI GTCTC 2 cut(s) 51, 815
BfaI CTAG 4 cut(s) 534, 555, 588, 1103
BfmI CTRYAG 2 cut(s) 500, 516
BfuAI ACCTGC 1 cut(s) 833
BisI GCNGC 6 cut(s) 57, 129, 672, 936, 1100, 1107
BlsI GCNGC 6 cut(s) 58, 130, 673, 937, 1101, 1108
Bme1390I CCNGG 3 cut(s) 74, 169, 316
Bme18I GGWCC 2 cut(s) 79, 1021
BmgT120I GGNCC 3 cut(s) 79, 113, 1021
BmiI GGNNCC 2 cut(s) 240, 1022
BmrFI CCNGG 3 cut(s) 74, 169, 316
BmsI GCATC 2 cut(s) 364, 919
BmtI GCTAGC 1 cut(s) 1106
BpuMI CCSGG 1 cut(s) 169
Bsa29I ATCGAT 1 cut(s) 1179
BsaBI GATNNNNATC 2 cut(s) 480, 627
BsaJI CCNNGG 2 cut(s) 167, 253
Bsc4I CCNNNNNNNGG 4 cut(s) 76, 88, 254, 842
Bse1I ACTGG 2 cut(s) 43, 577
Bse3DI GCAATG 1 cut(s) 265
Bse8I GATNNNNATC 2 cut(s) 480, 627
BseBI CCWGG 2 cut(s) 74, 316
BseCI ATCGAT 1 cut(s) 1179
BseDI CCNNGG 2 cut(s) 167, 253
BseGI GGATG 1 cut(s) 263
BseJI GATNNNNATC 2 cut(s) 480, 627
BseLI CCNNNNNNNGG 4 cut(s) 76, 88, 254, 842
BseMI GCAATG 1 cut(s) 265
BseMII CTCAG 3 cut(s) 62, 963, 1046
BseNI ACTGG 2 cut(s) 43, 577
BseXI GCAGC 5 cut(s) 115, 658, 922, 1086, 1118
BseYI CCCAGC 1 cut(s) 890
Bsh1285I CGRYCG 1 cut(s) 1183
BshFI GGCC 4 cut(s) 69, 92, 114, 701
BshVI ATCGAT 1 cut(s) 1179
BsiEI CGRYCG 1 cut(s) 1183
BsiHKAI GWGCWC 2 cut(s) 458, 554
BsiSI CCGG 3 cut(s) 89, 169, 191
BslI CCNNNNNNNGG 4 cut(s) 76, 88, 254, 842
BsmAI GTCTC 2 cut(s) 51, 815
BsmBI CGTCTC 1 cut(s) 815
BsmI GAATGC 2 cut(s) 160, 406
BsnI GGCC 4 cut(s) 69, 92, 114, 701
Bsp1286I GDGCHC 3 cut(s) 241, 458, 554
Bsp143I GATC 4 cut(s) 217, 481, 1176, 1180
BspACI CCGC 3 cut(s) 57, 814, 1164
BspANI GGCC 4 cut(s) 69, 92, 114, 701
BspCNI CTCAG 3 cut(s) 61, 964, 1045
BspDI ATCGAT 1 cut(s) 1179
BspLI GGNNCC 2 cut(s) 240, 1022
BspMI ACCTGC 1 cut(s) 833
BspOI GCTAGC 1 cut(s) 1106
BspPI GGATC 3 cut(s) 225, 489, 1184
BspQI GCTCTTC 1 cut(s) 270
BsrDI GCAATG 1 cut(s) 265
BsrI ACTGG 2 cut(s) 43, 577
BssECI CCNNGG 2 cut(s) 167, 253
BssMI GATC 4 cut(s) 217, 481, 1176, 1180
BssT1I CCWWGG 1 cut(s) 253
Bst2UI CCWGG 2 cut(s) 74, 316
Bst6I CTCTTC 2 cut(s) 270, 1080
BstC8I GCNNGC 3 cut(s) 67, 230, 1104
BstDEI CTNAG 3 cut(s) 48, 972, 1032
BstF5I GGATG 1 cut(s) 263
BstKTI GATC 4 cut(s) 220, 484, 1179, 1183
BstMAI GTCTC 2 cut(s) 51, 815
BstMBI GATC 4 cut(s) 217, 481, 1176, 1180
BstMCI CGRYCG 1 cut(s) 1183
BstMWI GCNNNNNNNGC 4 cut(s) 62, 120, 652, 944
BstNI CCWGG 2 cut(s) 74, 316
BstSCI CCNGG 3 cut(s) 72, 167, 314
BstSFI CTRYAG 2 cut(s) 500, 516
BstV1I GCAGC 5 cut(s) 115, 658, 922, 1086, 1118
Bsu15I ATCGAT 1 cut(s) 1179
BsuRI GGCC 4 cut(s) 69, 92, 114, 701
BsuTUI ATCGAT 1 cut(s) 1179
BtgZI GCGATG 1 cut(s) 282
BtsCI GGATG 1 cut(s) 263
BveI ACCTGC 1 cut(s) 833
Cac8I GCNNGC 3 cut(s) 67, 230, 1104
CaiI CAGNNNCTG 2 cut(s) 383, 605
Cfr13I GGNCC 3 cut(s) 79, 113, 1021
ClaI ATCGAT 1 cut(s) 1179
CsiI ACCWGGT 1 cut(s) 314
CviAII CATG 1 cut(s) 931
DdeI CTNAG 3 cut(s) 48, 972, 1032
DpnI GATC 4 cut(s) 219, 483, 1178, 1182
DpnII GATC 4 cut(s) 217, 481, 1176, 1180
Eam1104I CTCTTC 2 cut(s) 270, 1080
EarI CTCTTC 2 cut(s) 270, 1080
EciI GGCGGA 1 cut(s) 1179
Ecl136II GAGCTC 1 cut(s) 456
Eco130I CCWWGG 1 cut(s) 253
Eco24I GRGCYC 2 cut(s) 241, 458
Eco47I GGWCC 2 cut(s) 79, 1021
Eco53kI GAGCTC 1 cut(s) 456
Eco57I CTGAAG 2 cut(s) 294, 1104
EcoICRI GAGCTC 1 cut(s) 456
EcoO109I RGGNCCY 1 cut(s) 1021
EcoRII CCWGG 2 cut(s) 72, 314
EcoT14I CCWWGG 1 cut(s) 253
EcoT38I GRGCYC 2 cut(s) 241, 458
ErhI CCWWGG 1 cut(s) 253
Esp3I CGTCTC 1 cut(s) 815
FaeI CATG 1 cut(s) 934
FatI CATG 1 cut(s) 930
Fnu4HI GCNGC 6 cut(s) 57, 129, 672, 936, 1100, 1107
FokI GGATG 1 cut(s) 270
FriOI GRGCYC 2 cut(s) 241, 458
Fsp4HI GCNGC 6 cut(s) 57, 129, 672, 936, 1100, 1107
FspBI CTAG 4 cut(s) 534, 555, 588, 1103
GluI GCNGC 6 cut(s) 57, 129, 672, 936, 1100, 1107
GsaI CCCAGC 1 cut(s) 894
HaeIII GGCC 4 cut(s) 69, 92, 114, 701
HapII CCGG 3 cut(s) 89, 169, 191
Hin1II CATG 1 cut(s) 934
HincII GTYRAC 1 cut(s) 748
HindII GTYRAC 1 cut(s) 748
HindIII AAGCTT 3 cut(s) 5, 230, 756
HinfI GANTC 5 cut(s) 308, 449, 851, 1078, 1134
HpaII CCGG 3 cut(s) 89, 169, 191
HphI GGTGA 1 cut(s) 518
Hpy166II GTNNAC 4 cut(s) 610, 748, 1038, 1159
Hpy188I TCNGA 4 cut(s) 51, 148, 162, 622
Hpy188III TCNNGA 2 cut(s) 632, 1082
Hpy8I GTNNAC 4 cut(s) 610, 748, 1038, 1159
HpyAV CCTTC 5 cut(s) 103, 144, 191, 539, 712
HpyCH4IV ACGT 2 cut(s) 639, 736
HpyCH4V TGCA 5 cut(s) 178, 377, 655, 910, 989
HpyF10VI GCNNNNNNNGC 4 cut(s) 62, 120, 652, 944
HpyF3I CTNAG 3 cut(s) 48, 972, 1032
HpySE526I ACGT 2 cut(s) 639, 736
Hsp92II CATG 1 cut(s) 934
Kzo9I GATC 4 cut(s) 217, 481, 1176, 1180
LguI GCTCTTC 1 cut(s) 270
LmnI GCTCC 6 cut(s) 136, 163, 244, 461, 549, 887
Lsp1109I GCAGC 5 cut(s) 115, 658, 922, 1086, 1118
LweI GCATC 2 cut(s) 364, 919
MabI ACCWGGT 1 cut(s) 314
MaeI CTAG 4 cut(s) 534, 555, 588, 1103
MaeII ACGT 2 cut(s) 639, 736
MaeIII GTNAC 1 cut(s) 848
MalI GATC 4 cut(s) 219, 483, 1178, 1182
MboI GATC 4 cut(s) 217, 481, 1176, 1180
MboII GAAGA 6 cut(s) 133, 194, 287, 609, 905, 1097
MhlI GDGCHC 3 cut(s) 241, 458, 554
MluCI AATT 4 cut(s) 393, 539, 785, 873
MlyI GAGTC 3 cut(s) 845, 1072, 1128
MseI TTAA 5 cut(s) 119, 432, 522, 804, 1052
MspI CCGG 3 cut(s) 89, 169, 191
MspR9I CCNGG 3 cut(s) 74, 169, 316
Mva1269I GAATGC 2 cut(s) 160, 406
MvaI CCWGG 2 cut(s) 74, 316
MwoI GCNNNNNNNGC 4 cut(s) 62, 120, 652, 944
NciI CCSGG 1 cut(s) 169
NdeII GATC 4 cut(s) 217, 481, 1176, 1180
NheI GCTAGC 1 cut(s) 1102
NlaIII CATG 1 cut(s) 934
NlaIV GGNNCC 2 cut(s) 240, 1022
NmuCI GTSAC 1 cut(s) 848
PciSI GCTCTTC 1 cut(s) 270
PctI GAATGC 2 cut(s) 160, 406
PfeI GAWTC 2 cut(s) 308, 449
PkrI GCNGC 6 cut(s) 58, 130, 673, 937, 1101, 1108
Ple19I CGATCG 1 cut(s) 1183
PleI GAGTC 3 cut(s) 845, 1072, 1128
PpsI GAGTC 3 cut(s) 845, 1072, 1128
PpuMI RGGWCCY 1 cut(s) 1021
Psp124BI GAGCTC 1 cut(s) 458
Psp5II RGGWCCY 1 cut(s) 1021
Psp6I CCWGG 2 cut(s) 72, 314
PspFI CCCAGC 1 cut(s) 890
PspGI CCWGG 2 cut(s) 72, 314
PspN4I GGNNCC 2 cut(s) 240, 1022
PspPI GGNCC 3 cut(s) 79, 113, 1021
PspPPI RGGWCCY 1 cut(s) 1021
PstNI CAGNNNCTG 2 cut(s) 383, 605
PvuI CGATCG 1 cut(s) 1183
SacI GAGCTC 1 cut(s) 458
SapI GCTCTTC 1 cut(s) 270
SaqAI TTAA 5 cut(s) 119, 432, 522, 804, 1052
SatI GCNGC 6 cut(s) 57, 129, 672, 936, 1100, 1107
Sau3AI GATC 4 cut(s) 217, 481, 1176, 1180
Sau96I GGNCC 3 cut(s) 79, 113, 1021
SchI GAGTC 3 cut(s) 845, 1072, 1128
ScrFI CCNGG 3 cut(s) 74, 169, 316
SduI GDGCHC 3 cut(s) 241, 458, 554
SexAI ACCWGGT 1 cut(s) 314
SfaNI GCATC 2 cut(s) 364, 919
SfcI CTRYAG 2 cut(s) 500, 516
SinI GGWCC 2 cut(s) 79, 1021
Sse9I AATT 4 cut(s) 393, 539, 785, 873
SsiI CCGC 3 cut(s) 57, 814, 1164
SspMI CTAG 4 cut(s) 534, 555, 588, 1103
SstI GAGCTC 1 cut(s) 458
StyD4I CCNGG 3 cut(s) 72, 167, 314
StyI CCWWGG 1 cut(s) 253
TaiI ACGT 2 cut(s) 642, 739
TaqI TCGA 5 cut(s) 452, 705, 921, 1179, 1183
TasI AATT 4 cut(s) 393, 539, 785, 873
TauI GCSGC 1 cut(s) 59
TfiI GAWTC 2 cut(s) 308, 449
Tru1I TTAA 5 cut(s) 119, 432, 522, 804, 1052
Tru9I TTAA 5 cut(s) 119, 432, 522, 804, 1052
TseFI GTSAC 1 cut(s) 848
TseI GCWGC 5 cut(s) 128, 671, 935, 1099, 1106
Tsp45I GTSAC 1 cut(s) 848
TspDTI ATGAA 2 cut(s) 17, 885
TspGWI ACGGA 2 cut(s) 231, 354
VpaK11BI GGWCC 2 cut(s) 79, 1021
XapI RAATTY 2 cut(s) 393, 539
XspI CTAG 4 cut(s) 534, 555, 588, 1103
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.