RLG00000001065

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0008318
Physical Location & Seq
Forward (+)
10043666 .. 10046235
2570 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000001065

Sequence Viewer

Length: 1170 bp
ATGCAACTCTGGTTCTACCGCACCTTGAAGTTAATCCCGTGTGGCAAGATTCGAGGTATATATTCCTTTGAGGATATTTTTGATGTTGATCACTTTTTAGAAGTCCTACGCAATGAAGTTTCTATAGTTAAAGAACTTCCTGGTGATTTTTCTTGGAGCTCTAGGGAGTATTATGCTACAGGCATACGGCCTACTAGAATTAAAACGGCACCTGTTCATGCTCCTGCTGAGTGGTATCTGGAAAATGTCTTGCCCATAATGCAGAGTTATGGAGTTGCTGCTATTTCCCCGTTCTCCCATCGTTTAACTTTCGACAATTTGCCTGAAAGCATTCAGCGCCTACGTTGTAAAGTGAACTTTCAAGCTTTAGCCTTTGTTCCTTATATTAGGGAATTGGGAGAAACCCTTGTTAATCGTCTCCGCAACGCTCCTGTAAGAATCCAAAACGTAGAAACTGACTCACAAGAGGGAACAAATGACCTTGATAAACAGGGAGCTGGGAAGTTTGTTGTTTTGCATCTTCGCTTTGACAAAGATATGGCTGCTCATTCCGCCTGTGATTTTGGTGGTGGTAAAGCTGAGAAACTTGCTCTTGCAAAATATCGGCAAGTAATTTGGCAGGGAAGGGTCCTAAAATCTCAGTTCACAGATGAGGAGCTGAGAGTTCAGGGGCGTTGCCCGTTGACGCCTGAAGAGATTGGATTGTTGCTAGCAGCTCTGGGCTTCAACAATAGCACCCGGCTTTATCTTGCTTCCCACAAGGTTTATGGCGGAGAAGCAAGGATCTCAACTCTGCGCAGATTATTTCCACTATTGGAGGATAAGAAGAGCCTTACCTCCGCCGAGGAAAGGGCCAAGGTTGAAGGCAAGGCTTCTTTATTAGCTGCAGTTGACTATTATGTGAGCATGCAAAGTGACATCTTCATTTCTGCTTCTCCAGGAAATATGCACAATGCACTGGTGTGTCATCGAGCTTATATGAACTTGAAGACTATTAGACCGAGTATGACCCTGTTGAGTAAGCTATTCTTGAATAAGAGCATGCGGTGGTCAGAGTTCCAACATGCAGTTCTGGAAGGGCATAAAAACAGACAAGGGCAGATCAAGTTGAGAAAGGAGAAGCAATCAATATATACATATCCTGCTCCTGATTGCATGTGTCAAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

390

Amino Acids

44.33

Weight (kDa)

9.22

Isoelectric Point (pI)

43.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 22 - 327 6.3e-67 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 797
AccB1I GGYRCC 1 cut(s) 208
AciI CCGC 6 cut(s) 19, 421, 552, 771, 840, 1045
AclWI GGATC 1 cut(s) 791
AcuI CTGAAG 1 cut(s) 711
AcyI GRCGYC 1 cut(s) 686
AfiI CCNNNNNNNGG 1 cut(s) 849
AgsI TTSAA 6 cut(s) 28, 362, 727, 863, 988, 1033
AjnI CCWGG 2 cut(s) 139, 937
AjuI GAANNNNNNNTTGG 2 cut(s) 1053, 1085
AleI CACNNNNGTG 1 cut(s) 961
Alw21I GWGCWC 1 cut(s) 161
Alw26I GTCTC 1 cut(s) 422
AlwI GGATC 1 cut(s) 791
AoxI GGCC 2 cut(s) 188, 852
ApeKI GCWGC 4 cut(s) 278, 542, 713, 884
Asp700I GAANNNNTTC 1 cut(s) 330
AspLEI GCGC 2 cut(s) 339, 798
AspS9I GGNCC 2 cut(s) 628, 852
AsuC2I CCSGG 1 cut(s) 739
AsuHPI GGTGA 1 cut(s) 155
AsuNHI GCTAGC 1 cut(s) 709
AvaII GGWCC 1 cut(s) 628
BanI GGYRCC 1 cut(s) 208
BanII GRGCYC 1 cut(s) 161
BbsI GAAGAC 1 cut(s) 995
Bbv12I GWGCWC 1 cut(s) 161
BbvI GCAGC 4 cut(s) 265, 529, 725, 871
BccI CCATC 1 cut(s) 306
BceAI ACGGC 2 cut(s) 203, 222
BciT130I CCWGG 2 cut(s) 141, 939
BclI TGATCA 1 cut(s) 88
BcnI CCSGG 1 cut(s) 739
BcoDI GTCTC 1 cut(s) 422
BfaI CTAG 3 cut(s) 162, 195, 710
BfmI CTRYAG 3 cut(s) 123, 177, 885
BfoI RGCGCY 1 cut(s) 340
BisI GCNGC 4 cut(s) 279, 543, 714, 885
BlsI GCNGC 4 cut(s) 280, 544, 715, 886
Bme1390I CCNGG 3 cut(s) 141, 739, 939
Bme18I GGWCC 1 cut(s) 628
BmgT120I GGNCC 2 cut(s) 628, 852
BmiI GGNNCC 2 cut(s) 210, 629
BmrFI CCNGG 3 cut(s) 141, 739, 939
BmsI GCATC 1 cut(s) 526
BmtI GCTAGC 1 cut(s) 713
BpiI GAAGAC 1 cut(s) 995
BpmI CTGGAG 1 cut(s) 921
BpuMI CCSGG 1 cut(s) 739
BsaBI GATNNNNATC 1 cut(s) 87
BsaHI GRCGYC 1 cut(s) 686
BsaJI CCNNGG 2 cut(s) 843, 855
Bsc4I CCNNNNNNNGG 1 cut(s) 849
Bse1I ACTGG 1 cut(s) 963
Bse3DI GCAATG 1 cut(s) 118
Bse8I GATNNNNATC 1 cut(s) 87
BseBI CCWGG 2 cut(s) 141, 939
BseDI CCNNGG 2 cut(s) 843, 855
BseJI GATNNNNATC 1 cut(s) 87
BseLI CCNNNNNNNGG 1 cut(s) 849
BseMI GCAATG 1 cut(s) 118
BseMII CTCAG 4 cut(s) 219, 570, 650, 653
BseNI ACTGG 1 cut(s) 963
BseRI GAGGAG 1 cut(s) 668
BseXI GCAGC 4 cut(s) 265, 529, 725, 871
BseYI CCCAGC 1 cut(s) 497
BshFI GGCC 2 cut(s) 190, 854
BshNI GGYRCC 1 cut(s) 208
BsiHKAI GWGCWC 1 cut(s) 161
BsiSI CCGG 1 cut(s) 739
BslI CCNNNNNNNGG 1 cut(s) 849
BsmAI GTCTC 1 cut(s) 422
BsmBI CGTCTC 1 cut(s) 422
BsmI GAATGC 1 cut(s) 330
BsnI GGCC 2 cut(s) 190, 854
Bsp1286I GDGCHC 1 cut(s) 161
Bsp143I GATC 3 cut(s) 88, 783, 1101
BspACI CCGC 6 cut(s) 19, 421, 552, 771, 840, 1045
BspANI GGCC 2 cut(s) 190, 854
BspCNI CTCAG 4 cut(s) 220, 571, 651, 652
BspLI GGNNCC 2 cut(s) 210, 629
BspMAI CTGCAG 1 cut(s) 889
BspOI GCTAGC 1 cut(s) 713
BspPI GGATC 1 cut(s) 791
BspQI GCTCTTC 1 cut(s) 821
BspT107I GGYRCC 1 cut(s) 208
BsrDI GCAATG 1 cut(s) 118
BsrI ACTGG 1 cut(s) 963
BssECI CCNNGG 2 cut(s) 843, 855
BssMI GATC 3 cut(s) 88, 783, 1101
BssNI GRCGYC 1 cut(s) 686
BssT1I CCWWGG 1 cut(s) 855
Bst2UI CCWGG 2 cut(s) 141, 939
Bst6I CTCTTC 2 cut(s) 687, 821
BstACI GRCGYC 1 cut(s) 686
BstC8I GCNNGC 3 cut(s) 711, 908, 1043
BstDEI CTNAG 4 cut(s) 228, 579, 639, 659
BstH2I RGCGCY 1 cut(s) 340
BstHHI GCGC 2 cut(s) 339, 798
BstKTI GATC 3 cut(s) 91, 786, 1104
BstMAI GTCTC 1 cut(s) 422
BstMBI GATC 3 cut(s) 88, 783, 1101
BstMWI GCNNNNNNNGC 3 cut(s) 259, 336, 551
BstNI CCWGG 2 cut(s) 141, 939
BstNSI RCATGY 4 cut(s) 910, 1045, 1067, 1159
BstSCI CCNGG 3 cut(s) 139, 737, 937
BstSFI CTRYAG 3 cut(s) 123, 177, 885
BstV1I GCAGC 4 cut(s) 265, 529, 725, 871
BstV2I GAAGAC 1 cut(s) 995
BstX2I RGATCY 1 cut(s) 783
BstYI RGATCY 1 cut(s) 783
BsuRI GGCC 2 cut(s) 190, 854
BtsIMutI CAGTG 1 cut(s) 956
Cac8I GCNNGC 3 cut(s) 711, 908, 1043
CfoI GCGC 2 cut(s) 339, 798
Cfr13I GGNCC 2 cut(s) 628, 852
CseI GACGC 1 cut(s) 694
CviAII CATG 5 cut(s) 218, 907, 1042, 1064, 1156
DdeI CTNAG 4 cut(s) 228, 579, 639, 659
DpnI GATC 3 cut(s) 90, 785, 1103
DpnII GATC 3 cut(s) 88, 783, 1101
Eam1104I CTCTTC 2 cut(s) 687, 821
EarI CTCTTC 2 cut(s) 687, 821
EciI GGCGGA 3 cut(s) 541, 786, 829
Ecl136II GAGCTC 1 cut(s) 159
Eco130I CCWWGG 1 cut(s) 855
Eco24I GRGCYC 1 cut(s) 161
Eco47I GGWCC 1 cut(s) 628
Eco53kI GAGCTC 1 cut(s) 159
Eco57I CTGAAG 1 cut(s) 711
EcoICRI GAGCTC 1 cut(s) 159
EcoO109I RGGNCCY 1 cut(s) 628
EcoRII CCWGG 2 cut(s) 139, 937
EcoT14I CCWWGG 1 cut(s) 855
EcoT38I GRGCYC 1 cut(s) 161
ErhI CCWWGG 1 cut(s) 855
Esp3I CGTCTC 1 cut(s) 422
FaeI CATG 5 cut(s) 221, 910, 1045, 1067, 1159
FalI AAGNNNNNCTT 2 cut(s) 1013, 1045
FatI CATG 5 cut(s) 217, 906, 1041, 1063, 1155
FbaI TGATCA 1 cut(s) 88
Fnu4HI GCNGC 4 cut(s) 279, 543, 714, 885
FriOI GRGCYC 1 cut(s) 161
Fsp4HI GCNGC 4 cut(s) 279, 543, 714, 885
FspBI CTAG 3 cut(s) 162, 195, 710
FspI TGCGCA 1 cut(s) 797
GlaI GCGC 2 cut(s) 338, 797
GluI GCNGC 4 cut(s) 279, 543, 714, 885
GsaI CCCAGC 1 cut(s) 501
GsuI CTGGAG 1 cut(s) 921
HaeII RGCGCY 1 cut(s) 340
HaeIII GGCC 2 cut(s) 190, 854
HapII CCGG 1 cut(s) 739
HgaI GACGC 1 cut(s) 694
HhaI GCGC 2 cut(s) 339, 798
Hin1I GRCGYC 1 cut(s) 686
Hin1II CATG 5 cut(s) 221, 910, 1045, 1067, 1159
Hin6I GCGC 2 cut(s) 337, 796
HinP1I GCGC 2 cut(s) 337, 796
HincII GTYRAC 2 cut(s) 684, 892
HindII GTYRAC 2 cut(s) 684, 892
HindIII AAGCTT 2 cut(s) 363, 1164
HinfI GANTC 3 cut(s) 49, 438, 458
HpaII CCGG 1 cut(s) 739
HphI GGTGA 1 cut(s) 155
Hpy166II GTNNAC 4 cut(s) 355, 645, 684, 892
Hpy188I TCNGA 1 cut(s) 1054
Hpy188III TCNNGA 4 cut(s) 239, 1030, 1073, 1148
Hpy8I GTNNAC 4 cut(s) 355, 645, 684, 892
HpyAV CCTTC 3 cut(s) 618, 857, 1070
HpyCH4IV ACGT 2 cut(s) 343, 447
HpyF10VI GCNNNNNNNGC 3 cut(s) 259, 336, 551
HpyF3I CTNAG 4 cut(s) 228, 579, 639, 659
HpySE526I ACGT 2 cut(s) 343, 447
Hsp92I GRCGYC 1 cut(s) 686
Hsp92II CATG 5 cut(s) 221, 910, 1045, 1067, 1159
HspAI GCGC 2 cut(s) 337, 796
Ksp22I TGATCA 1 cut(s) 88
Kzo9I GATC 3 cut(s) 88, 783, 1101
LguI GCTCTTC 1 cut(s) 821
LmnI GCTCC 6 cut(s) 156, 226, 433, 494, 655, 1150
Lsp1109I GCAGC 4 cut(s) 265, 529, 725, 871
LweI GCATC 1 cut(s) 526
MaeI CTAG 3 cut(s) 162, 195, 710
MaeII ACGT 2 cut(s) 343, 447
MaeIII GTNAC 1 cut(s) 914
MalI GATC 3 cut(s) 90, 785, 1103
MboI GATC 3 cut(s) 88, 783, 1101
MboII GAAGA 5 cut(s) 512, 704, 838, 913, 1000
MflI RGATCY 1 cut(s) 783
MhlI GDGCHC 1 cut(s) 161
MluCI AATT 4 cut(s) 198, 316, 392, 612
MlyI GAGTC 1 cut(s) 452
MmeI TCCRAC 1 cut(s) 1084
MnlI CCTC 7 cut(s) 47, 64, 460, 646, 811, 838, 847
MroXI GAANNNNTTC 1 cut(s) 330
MseI TTAA 6 cut(s) 32, 129, 201, 305, 411, 1168
MslI CAYNNNNRTG 1 cut(s) 961
MspI CCGG 1 cut(s) 739
MspR9I CCNGG 3 cut(s) 141, 739, 939
Mva1269I GAATGC 1 cut(s) 330
MvaI CCWGG 2 cut(s) 141, 939
MwoI GCNNNNNNNGC 3 cut(s) 259, 336, 551
NciI CCSGG 1 cut(s) 739
NdeII GATC 3 cut(s) 88, 783, 1101
NheI GCTAGC 1 cut(s) 709
NlaIII CATG 5 cut(s) 221, 910, 1045, 1067, 1159
NlaIV GGNNCC 2 cut(s) 210, 629
NmeAIII GCCGAG 1 cut(s) 868
NmuCI GTSAC 1 cut(s) 914
NsbI TGCGCA 1 cut(s) 797
NspI RCATGY 4 cut(s) 910, 1045, 1067, 1159
OliI CACNNNNGTG 1 cut(s) 961
PaeI GCATGC 2 cut(s) 910, 1045
PciSI GCTCTTC 1 cut(s) 821
PctI GAATGC 1 cut(s) 330
PdmI GAANNNNTTC 1 cut(s) 330
PfeI GAWTC 2 cut(s) 49, 438
PfoI TCCNGGA 1 cut(s) 937
PkrI GCNGC 4 cut(s) 280, 544, 715, 886
PleI GAGTC 1 cut(s) 452
PpsI GAGTC 1 cut(s) 452
PpuMI RGGWCCY 1 cut(s) 628
Psp124BI GAGCTC 1 cut(s) 161
Psp5II RGGWCCY 1 cut(s) 628
Psp6I CCWGG 2 cut(s) 139, 937
PspFI CCCAGC 1 cut(s) 497
PspGI CCWGG 2 cut(s) 139, 937
PspN4I GGNNCC 2 cut(s) 210, 629
PspPI GGNCC 2 cut(s) 628, 852
PspPPI RGGWCCY 1 cut(s) 628
PstI CTGCAG 1 cut(s) 889
PsuI RGATCY 1 cut(s) 783
RseI CAYNNNNRTG 1 cut(s) 961
SacI GAGCTC 1 cut(s) 161
SapI GCTCTTC 1 cut(s) 821
SaqAI TTAA 6 cut(s) 32, 129, 201, 305, 411, 1168
SatI GCNGC 4 cut(s) 279, 543, 714, 885
Sau3AI GATC 3 cut(s) 88, 783, 1101
Sau96I GGNCC 2 cut(s) 628, 852
SchI GAGTC 1 cut(s) 452
ScrFI CCNGG 3 cut(s) 141, 739, 939
SduI GDGCHC 1 cut(s) 161
SfaNI GCATC 1 cut(s) 526
SfcI CTRYAG 3 cut(s) 123, 177, 885
SinI GGWCC 1 cut(s) 628
SmiMI CAYNNNNRTG 1 cut(s) 961
SphI GCATGC 2 cut(s) 910, 1045
Sse9I AATT 4 cut(s) 198, 316, 392, 612
SsiI CCGC 6 cut(s) 19, 421, 552, 771, 840, 1045
SspMI CTAG 3 cut(s) 162, 195, 710
SstI GAGCTC 1 cut(s) 161
StyD4I CCNGG 3 cut(s) 139, 737, 937
StyI CCWWGG 1 cut(s) 855
TaiI ACGT 2 cut(s) 346, 450
TaqI TCGA 3 cut(s) 52, 312, 970
TaqII GACCGA 1 cut(s) 1015
TasI AATT 4 cut(s) 198, 316, 392, 612
TfiI GAWTC 2 cut(s) 49, 438
Tru1I TTAA 6 cut(s) 32, 129, 201, 305, 411, 1168
Tru9I TTAA 6 cut(s) 32, 129, 201, 305, 411, 1168
TscAI CASTG 1 cut(s) 963
TseFI GTSAC 1 cut(s) 914
TseI GCWGC 4 cut(s) 278, 542, 713, 884
Tsp45I GTSAC 1 cut(s) 914
TspDTI ATGAA 4 cut(s) 129, 206, 913, 995
TspRI CASTG 1 cut(s) 963
VpaK11BI GGWCC 1 cut(s) 628
XceI RCATGY 4 cut(s) 910, 1045, 1067, 1159
XcmI CCANNNNNNNNNTGG 1 cut(s) 764
XmnI GAANNNNTTC 1 cut(s) 330
XspI CTAG 3 cut(s) 162, 195, 710
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.