RchiOBHm_Chr7g0236731

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0008318
Physical Location & Seq
Reverse (-)
61406937 .. 61411206
4270 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ21215

Sequence Viewer

Length: 1557 bp
ATGAAGCTCTACAAAGACTACTACTTCCACCCCAACCACTCTCAGAGAGCCGCCTTGGCTGCCCTCTTCGTCCTTCTCTTCCCCACCTTCTTCCCCAATCTCTTCGGCCCATTGGGCCGCGCCTCTCCTTCCATCTTCTCTGAATGGAATGCTCCAAAGCCCAGGCATTTACATCTTCTAGAAGCAGCCTTGTCAACCCAATCTTCCGTTGAGCATCAGATCAAGGTTTGGGCTCCGCTGCCCAATCAGGGATGGAAGCATTGCCCTGAAGAGCCTAGAAGTGCTTCATCGAAACCGAAAGGATCAAATGGGTATATACAGGTGTTCCTTGATGGAGGCTTGAATCAGCAGAAAATGGGGATTTGTGATGCGGTGGCTGTTGCTAAAATCTTGAATGCAACTCTGGTTCTACCGCACCTTGAAGTTAATCCCGTGTGGCAAGATTCGAGTTCCTTTGAGGAAATTTTTGATGTCGATCACTTTTTAGAAGTCCTACGCAATGAAGTTTCTATAGTTAAAGAACTTCCTAGTGAGTTTTCTTGGAGCTCTAGGGAGTATTATGCTACAGGCATACGGCCTACTAGAATTAAAACGGCACCTGTTCATGCTCCTGCTGAGTGGTATCTGGAAAATGTCTTGCCCATAATGCAGAGTTATGGAGTTGCTGCTATTTCCCCGTTCTCCCATCGTTTAACTTTCGACAATTTGCCCGAAAGCATTCAGCGCCTACGTTGTAAAGTGAACTTTCAAGCTTTAGCCTTTGTTCCTCATATTAGGGAATTGGGAGAAACCCTTGTTAATCGTCTCCGCAACCCTCCTGTAAGAATCCAAAACGCAGAAACTGACTCACAAGAGGGAACAAATGACCTTGATAAACAGGGAGCTGGGAAGTTTGTTGTTTTGCATCTTCGCTTTGACAAAGATATGGCTGCTCATTCCGCCTGTGATTTTGGTGGCGGTAAAGCTGAGAAACTTGCTCTTGCAAAATATCGACAAGTAATTTGGCAGGGAAGGGTCCTAAAATCTCAGTTCTCAGATGAGGAGCTGAGAGTTCAGGGGCGTTGCCCGTTGACGCCTGAAGAGATTGGATTGTTGCTAGCAGCTCTGGGCTTCAACAATAGCACCCGGCTTTATCTTGCTTCCCACAAGGTTTATGGTGGAGAAGCAAGGATCTCAACTCTGAGAAGATTATTTCCACTACTGGAGGATAAGAAGAGCCTTACCTCCGCCGAGAAAAGGGCTAAGGTTGAAGGCAAGGCTTCTCTGTTAGCTGCTGTTGACTATTATGTGAGCATGCAAAGTGACATCTTCATTTCTGCTTCTCCGGGAAATATGCACAATGCACTGGTGTGTCATCGAGCTTATATGAACTTGAAGACTATTAGACCAAGCATGACCCTGTTGAGTAAGCTGTTCTTGAATAAGAGCATGCAGTGGTCAGAATTCCAACATGCAGTTTTGGAAGGGCATAAAAACAGACAAGGGCAGATCAAGTTGAGAAAGGAGAAGCAATCCATATATACATATCCTGCTCCTGATTGCATGTGTCAAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

518

Amino Acids

58.43

Weight (kDa)

9.11

Isoelectric Point (pI)

47.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 105 - 456 1e-88 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 595
AccII CGCG 1 cut(s) 120
AciI CCGC 9 cut(s) 51, 118, 236, 371, 413, 808, 939, 957, 1227
AclWI GGATC 2 cut(s) 310, 1178
AcsI RAATTY 2 cut(s) 462, 1442
AcuI CTGAAG 2 cut(s) 288, 1098
AcyI GRCGYC 1 cut(s) 1073
AfiI CCNNNNNNNGG 2 cut(s) 248, 1236
AgsI TTSAA 8 cut(s) 343, 394, 422, 749, 1114, 1250, 1375, 1420
AjnI CCWGG 1 cut(s) 161
AleI CACNNNNGTG 1 cut(s) 1348
Alw21I GWGCWC 1 cut(s) 548
Alw26I GTCTC 1 cut(s) 809
AlwI GGATC 2 cut(s) 310, 1178
AlwNI CAGNNNCTG 1 cut(s) 842
AoxI GGCC 3 cut(s) 106, 115, 575
ApeKI GCWGC 7 cut(s) 59, 185, 238, 665, 929, 1100, 1271
ApoI RAATTY 2 cut(s) 462, 1442
ArsI GACNNNNNNTTYG 2 cut(s) 984, 1016
Asp700I GAANNNNTTC 2 cut(s) 283, 717
AspLEI GCGC 2 cut(s) 122, 726
AspS9I GGNCC 3 cut(s) 107, 115, 1015
AsuC2I CCSGG 2 cut(s) 1126, 1326
AsuNHI GCTAGC 1 cut(s) 1096
AvaII GGWCC 1 cut(s) 1015
BanI GGYRCC 1 cut(s) 595
BanII GRGCYC 2 cut(s) 235, 548
BbsI GAAGAC 1 cut(s) 1382
Bbv12I GWGCWC 1 cut(s) 548
BbvI GCAGC 7 cut(s) 46, 197, 225, 652, 916, 1112, 1258
BccI CCATC 4 cut(s) 140, 246, 326, 693
BceAI ACGGC 2 cut(s) 590, 609
BciT130I CCWGG 1 cut(s) 163
BcnI CCSGG 2 cut(s) 1126, 1326
BcoDI GTCTC 1 cut(s) 809
BfaI CTAG 6 cut(s) 179, 276, 528, 549, 582, 1097
BfmI CTRYAG 2 cut(s) 510, 564
BfoI RGCGCY 1 cut(s) 727
BglI GCCNNNNNGGC 2 cut(s) 56, 114
BisI GCNGC 9 cut(s) 51, 60, 118, 186, 239, 666, 930, 1101, 1272
BlsI GCNGC 9 cut(s) 52, 61, 119, 187, 240, 667, 931, 1102, 1273
Bme1390I CCNGG 3 cut(s) 163, 1126, 1326
Bme18I GGWCC 1 cut(s) 1015
BmgT120I GGNCC 3 cut(s) 107, 115, 1015
BmiI GGNNCC 3 cut(s) 234, 597, 1016
BmrFI CCNGG 3 cut(s) 163, 1126, 1326
BmsI GCATC 3 cut(s) 223, 358, 913
BmtI GCTAGC 1 cut(s) 1100
BpiI GAAGAC 1 cut(s) 1382
BpmI CTGGAG 1 cut(s) 1223
Bpu10I CCTNAGC 1 cut(s) 1242
BpuMI CCSGG 2 cut(s) 1126, 1326
BsaBI GATNNNNATC 1 cut(s) 474
BsaHI GRCGYC 1 cut(s) 1073
BsaJI CCNNGG 2 cut(s) 54, 161
Bsc4I CCNNNNNNNGG 2 cut(s) 248, 1236
Bse1I ACTGG 2 cut(s) 1206, 1350
Bse3DI GCAATG 2 cut(s) 259, 505
Bse8I GATNNNNATC 1 cut(s) 474
BseBI CCWGG 1 cut(s) 163
BseDI CCNNGG 2 cut(s) 54, 161
BseGI GGATG 1 cut(s) 257
BseJI GATNNNNATC 1 cut(s) 474
BseLI CCNNNNNNNGG 2 cut(s) 248, 1236
BseMI GCAATG 2 cut(s) 259, 505
BseMII CTCAG 7 cut(s) 56, 606, 957, 1037, 1040, 1047, 1172
BseNI ACTGG 2 cut(s) 1206, 1350
BseRI GAGGAG 1 cut(s) 1055
BseXI GCAGC 7 cut(s) 46, 197, 225, 652, 916, 1112, 1258
BseYI CCCAGC 1 cut(s) 884
Bsh1236I CGCG 1 cut(s) 120
BshFI GGCC 3 cut(s) 108, 117, 577
BshNI GGYRCC 1 cut(s) 595
BsiHKAI GWGCWC 1 cut(s) 548
BsiSI CCGG 2 cut(s) 1126, 1325
BslI CCNNNNNNNGG 2 cut(s) 248, 1236
BsmAI GTCTC 1 cut(s) 809
BsmBI CGTCTC 1 cut(s) 809
BsmI GAATGC 3 cut(s) 154, 400, 717
BsnI GGCC 3 cut(s) 108, 117, 577
Bsp1286I GDGCHC 2 cut(s) 235, 548
Bsp143I GATC 5 cut(s) 219, 302, 475, 1170, 1488
BspACI CCGC 9 cut(s) 51, 118, 236, 371, 413, 808, 939, 957, 1227
BspANI GGCC 3 cut(s) 108, 117, 577
BspCNI CTCAG 7 cut(s) 55, 607, 958, 1038, 1039, 1046, 1173
BspFNI CGCG 1 cut(s) 120
BspLI GGNNCC 3 cut(s) 234, 597, 1016
BspOI GCTAGC 1 cut(s) 1100
BspPI GGATC 2 cut(s) 310, 1178
BspQI GCTCTTC 2 cut(s) 264, 1208
BspT107I GGYRCC 1 cut(s) 595
BsrDI GCAATG 2 cut(s) 259, 505
BsrI ACTGG 2 cut(s) 1206, 1350
BssECI CCNNGG 2 cut(s) 54, 161
BssMI GATC 5 cut(s) 219, 302, 475, 1170, 1488
BssNI GRCGYC 1 cut(s) 1073
BssT1I CCWWGG 1 cut(s) 54
Bst2UI CCWGG 1 cut(s) 163
Bst6I CTCTTC 6 cut(s) 71, 83, 107, 264, 1074, 1208
BstACI GRCGYC 1 cut(s) 1073
BstC8I GCNNGC 3 cut(s) 1098, 1295, 1430
BstDEI CTNAG 8 cut(s) 42, 615, 966, 1026, 1033, 1046, 1181, 1242
BstF5I GGATG 1 cut(s) 257
BstFNI CGCG 1 cut(s) 120
BstH2I RGCGCY 1 cut(s) 727
BstHHI GCGC 2 cut(s) 122, 726
BstKTI GATC 5 cut(s) 222, 305, 478, 1173, 1491
BstMAI GTCTC 1 cut(s) 809
BstMBI GATC 5 cut(s) 219, 302, 475, 1170, 1488
BstMWI GCNNNNNNNGC 6 cut(s) 56, 59, 114, 646, 723, 938
BstNI CCWGG 1 cut(s) 163
BstNSI RCATGY 4 cut(s) 1297, 1432, 1454, 1546
BstSCI CCNGG 3 cut(s) 161, 1124, 1324
BstSFI CTRYAG 2 cut(s) 510, 564
BstUI CGCG 1 cut(s) 120
BstV1I GCAGC 7 cut(s) 46, 197, 225, 652, 916, 1112, 1258
BstV2I GAAGAC 1 cut(s) 1382
BstX2I RGATCY 1 cut(s) 1170
BstYI RGATCY 1 cut(s) 1170
BsuRI GGCC 3 cut(s) 108, 117, 577
BtsCI GGATG 1 cut(s) 257
BtsI GCAGTG 1 cut(s) 1439
BtsIMutI CAGTG 2 cut(s) 1343, 1439
Cac8I GCNNGC 3 cut(s) 1098, 1295, 1430
CaiI CAGNNNCTG 1 cut(s) 842
CfoI GCGC 2 cut(s) 122, 726
Cfr13I GGNCC 3 cut(s) 107, 115, 1015
CseI GACGC 1 cut(s) 1081
CviAII CATG 6 cut(s) 605, 1294, 1393, 1429, 1451, 1543
DdeI CTNAG 8 cut(s) 42, 615, 966, 1026, 1033, 1046, 1181, 1242
DpnI GATC 5 cut(s) 221, 304, 477, 1172, 1490
DpnII GATC 5 cut(s) 219, 302, 475, 1170, 1488
Eam1104I CTCTTC 6 cut(s) 71, 83, 107, 264, 1074, 1208
EarI CTCTTC 6 cut(s) 71, 83, 107, 264, 1074, 1208
EciI GGCGGA 2 cut(s) 928, 1216
Ecl136II GAGCTC 1 cut(s) 546
Eco130I CCWWGG 1 cut(s) 54
Eco24I GRGCYC 2 cut(s) 235, 548
Eco47I GGWCC 1 cut(s) 1015
Eco53kI GAGCTC 1 cut(s) 546
Eco57I CTGAAG 2 cut(s) 288, 1098
EcoICRI GAGCTC 1 cut(s) 546
EcoO109I RGGNCCY 1 cut(s) 1015
EcoRI GAATTC 1 cut(s) 1442
EcoRII CCWGG 1 cut(s) 161
EcoT14I CCWWGG 1 cut(s) 54
EcoT38I GRGCYC 2 cut(s) 235, 548
ErhI CCWWGG 1 cut(s) 54
Esp3I CGTCTC 1 cut(s) 809
FaeI CATG 6 cut(s) 608, 1297, 1396, 1432, 1454, 1546
FalI AAGNNNNNCTT 2 cut(s) 1400, 1432
FatI CATG 6 cut(s) 604, 1293, 1392, 1428, 1450, 1542
Fnu4HI GCNGC 9 cut(s) 51, 60, 118, 186, 239, 666, 930, 1101, 1272
FokI GGATG 1 cut(s) 264
FriOI GRGCYC 2 cut(s) 235, 548
Fsp4HI GCNGC 9 cut(s) 51, 60, 118, 186, 239, 666, 930, 1101, 1272
FspBI CTAG 6 cut(s) 179, 276, 528, 549, 582, 1097
GlaI GCGC 2 cut(s) 121, 725
GluI GCNGC 9 cut(s) 51, 60, 118, 186, 239, 666, 930, 1101, 1272
GsaI CCCAGC 1 cut(s) 888
GsuI CTGGAG 1 cut(s) 1223
HaeII RGCGCY 1 cut(s) 727
HaeIII GGCC 3 cut(s) 108, 117, 577
HapII CCGG 2 cut(s) 1126, 1325
HgaI GACGC 1 cut(s) 1081
HhaI GCGC 2 cut(s) 122, 726
Hin1I GRCGYC 1 cut(s) 1073
Hin1II CATG 6 cut(s) 608, 1297, 1396, 1432, 1454, 1546
Hin6I GCGC 2 cut(s) 120, 724
HinP1I GCGC 2 cut(s) 120, 724
HincII GTYRAC 3 cut(s) 195, 1071, 1279
HindII GTYRAC 3 cut(s) 195, 1071, 1279
HindIII AAGCTT 2 cut(s) 750, 1551
HinfI GANTC 4 cut(s) 343, 443, 825, 845
HpaII CCGG 2 cut(s) 1126, 1325
Hpy166II GTNNAC 4 cut(s) 195, 742, 1071, 1279
Hpy188I TCNGA 6 cut(s) 45, 142, 219, 1036, 1182, 1441
Hpy188III TCNNGA 5 cut(s) 179, 391, 626, 1417, 1535
Hpy8I GTNNAC 4 cut(s) 195, 742, 1071, 1279
HpyAV CCTTC 6 cut(s) 83, 97, 138, 1005, 1244, 1457
HpyCH4IV ACGT 1 cut(s) 730
HpyF10VI GCNNNNNNNGC 6 cut(s) 56, 59, 114, 646, 723, 938
HpyF3I CTNAG 8 cut(s) 42, 615, 966, 1026, 1033, 1046, 1181, 1242
HpySE526I ACGT 1 cut(s) 730
Hsp92I GRCGYC 1 cut(s) 1073
Hsp92II CATG 6 cut(s) 608, 1297, 1396, 1432, 1454, 1546
HspAI GCGC 2 cut(s) 120, 724
Kzo9I GATC 5 cut(s) 219, 302, 475, 1170, 1488
LguI GCTCTTC 2 cut(s) 264, 1208
LmnI GCTCC 7 cut(s) 157, 238, 543, 613, 881, 1042, 1537
Lsp1109I GCAGC 7 cut(s) 46, 197, 225, 652, 916, 1112, 1258
LweI GCATC 3 cut(s) 223, 358, 913
MaeI CTAG 6 cut(s) 179, 276, 528, 549, 582, 1097
MaeII ACGT 1 cut(s) 730
MaeIII GTNAC 1 cut(s) 1301
MalI GATC 5 cut(s) 221, 304, 477, 1172, 1490
MboI GATC 5 cut(s) 219, 302, 475, 1170, 1488
MflI RGATCY 1 cut(s) 1170
MhlI GDGCHC 2 cut(s) 235, 548
MluCI AATT 6 cut(s) 462, 585, 703, 779, 999, 1442
MlyI GAGTC 1 cut(s) 839
MmeI TCCRAC 1 cut(s) 1471
MroXI GAANNNNTTC 2 cut(s) 283, 717
MseI TTAA 6 cut(s) 426, 516, 588, 692, 798, 1555
MslI CAYNNNNRTG 1 cut(s) 1348
MspA1I CMGCKG 1 cut(s) 238
MspI CCGG 2 cut(s) 1126, 1325
MspR9I CCNGG 3 cut(s) 163, 1126, 1326
Mva1269I GAATGC 3 cut(s) 154, 400, 717
MvaI CCWGG 1 cut(s) 163
MvnI CGCG 1 cut(s) 120
MwoI GCNNNNNNNGC 6 cut(s) 56, 59, 114, 646, 723, 938
NciI CCSGG 2 cut(s) 1126, 1326
NdeII GATC 5 cut(s) 219, 302, 475, 1170, 1488
NheI GCTAGC 1 cut(s) 1096
NlaIII CATG 6 cut(s) 608, 1297, 1396, 1432, 1454, 1546
NlaIV GGNNCC 3 cut(s) 234, 597, 1016
NmeAIII GCCGAG 1 cut(s) 1255
NmuCI GTSAC 1 cut(s) 1301
NspI RCATGY 4 cut(s) 1297, 1432, 1454, 1546
OliI CACNNNNGTG 1 cut(s) 1348
PaeI GCATGC 2 cut(s) 1297, 1432
PciSI GCTCTTC 2 cut(s) 264, 1208
PctI GAATGC 3 cut(s) 154, 400, 717
PdmI GAANNNNTTC 2 cut(s) 283, 717
PfeI GAWTC 3 cut(s) 343, 443, 825
PfoI TCCNGGA 1 cut(s) 1324
PkrI GCNGC 9 cut(s) 52, 61, 119, 187, 240, 667, 931, 1102, 1273
PleI GAGTC 1 cut(s) 839
PpsI GAGTC 1 cut(s) 839
PpuMI RGGWCCY 1 cut(s) 1015
Psp124BI GAGCTC 1 cut(s) 548
Psp5II RGGWCCY 1 cut(s) 1015
Psp6I CCWGG 1 cut(s) 161
PspFI CCCAGC 1 cut(s) 884
PspGI CCWGG 1 cut(s) 161
PspN4I GGNNCC 3 cut(s) 234, 597, 1016
PspPI GGNCC 3 cut(s) 107, 115, 1015
PspPPI RGGWCCY 1 cut(s) 1015
PstNI CAGNNNCTG 1 cut(s) 842
PsuI RGATCY 1 cut(s) 1170
RseI CAYNNNNRTG 1 cut(s) 1348
SacI GAGCTC 1 cut(s) 548
SapI GCTCTTC 2 cut(s) 264, 1208
SaqAI TTAA 6 cut(s) 426, 516, 588, 692, 798, 1555
SatI GCNGC 9 cut(s) 51, 60, 118, 186, 239, 666, 930, 1101, 1272
Sau3AI GATC 5 cut(s) 219, 302, 475, 1170, 1488
Sau96I GGNCC 3 cut(s) 107, 115, 1015
SchI GAGTC 1 cut(s) 839
ScrFI CCNGG 3 cut(s) 163, 1126, 1326
SduI GDGCHC 2 cut(s) 235, 548
SfaNI GCATC 3 cut(s) 223, 358, 913
SfcI CTRYAG 2 cut(s) 510, 564
SfiI GGCCNNNNNGGCC 1 cut(s) 114
SinI GGWCC 1 cut(s) 1015
SmiMI CAYNNNNRTG 1 cut(s) 1348
SphI GCATGC 2 cut(s) 1297, 1432
Sse9I AATT 6 cut(s) 462, 585, 703, 779, 999, 1442
SsiI CCGC 9 cut(s) 51, 118, 236, 371, 413, 808, 939, 957, 1227
SspMI CTAG 6 cut(s) 179, 276, 528, 549, 582, 1097
SstI GAGCTC 1 cut(s) 548
StyD4I CCNGG 3 cut(s) 161, 1124, 1324
StyI CCWWGG 1 cut(s) 54
TaiI ACGT 1 cut(s) 733
TaqI TCGA 6 cut(s) 290, 446, 474, 699, 991, 1357
TasI AATT 6 cut(s) 462, 585, 703, 779, 999, 1442
TauI GCSGC 2 cut(s) 53, 120
TfiI GAWTC 3 cut(s) 343, 443, 825
Tru1I TTAA 6 cut(s) 426, 516, 588, 692, 798, 1555
Tru9I TTAA 6 cut(s) 426, 516, 588, 692, 798, 1555
TscAI CASTG 2 cut(s) 1350, 1439
TseFI GTSAC 1 cut(s) 1301
TseI GCWGC 7 cut(s) 59, 185, 238, 665, 929, 1100, 1271
Tsp45I GTSAC 1 cut(s) 1301
TspDTI ATGAA 6 cut(s) 17, 276, 516, 593, 1300, 1382
TspGWI ACGGA 1 cut(s) 196
TspRI CASTG 2 cut(s) 1350, 1439
VpaK11BI GGWCC 1 cut(s) 1015
XapI RAATTY 2 cut(s) 462, 1442
XbaI TCTAGA 1 cut(s) 178
XceI RCATGY 4 cut(s) 1297, 1432, 1454, 1546
XcmI CCANNNNNNNNNTGG 1 cut(s) 1151
XmnI GAANNNNTTC 2 cut(s) 283, 717
XspI CTAG 6 cut(s) 179, 276, 528, 549, 582, 1097
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.