MD15G1385100.v1.1

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0008318
Physical Location & Seq
Forward (+)
47574418 .. 47577616
3199 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1385100.v1.1.491

Sequence Viewer

Length: 1011 bp
ATGAAGCTTCACAAAGACTACTATGGCTACTACTACCCCAACCAGTCTCAGAGAGCCGCTTTAGCCGGCCTTTTGGTGGTCCTCTTTCCGGCCTTCCTGCCAAACCTCTTTGGCCCATTGGGCCACGCCTCTCCTTCCATCTTCTCGGAGTGGAATGCCCCGCAACCCCGGCATTCGCGCCTTCTAAGTCCGGCTTTACTTCTTCAATCTTCTGTGGATCAACAGAGCAAGCTTTGGGCTCCGTTGCCCGACCAAGGATGGAAGCATTGTCCTGAAGAGCCTAGAAGTTTATCATCGTCGCCAAAAGAATCTACTGGGTATATTCAGGTGTTCCTTGATGGAGGCTTGAACCAGCAGAAAATGGGGATTTGTGATGCAGTTGCTGTTGCTAAAATTTTGAATGCCACCTTGGTTATTCCACACCTTGAAGTTAATCCTGTGTGGCAAGATTCGAGTTCCTTTGAGGACATTTTTGATGTGGGTCACTTTATTGAAGCCCTACAGGGTGAAGTTTCTATAGTTAAAGCCCTTCCTATTGAATTTTCTTGGAGCACTAGGGAGTATTATGCTACTGGCATACGGATTACAAGAATAAAAACAGCACCTGTTCATGCTTCTTCTGATTGGTATCTGGAAAACGTCCTGCCCATATTGCAGAGATATGGAGTTGCTGCTATCTCCCCATTTTCTCATCGTTTGGCTTTTGAAAACTTGCCTCCAAGCATACAGCACCTACGTTGTAAAGTCAACTTTGAAGCTTTAGCCTTTGTTCCTCATATCAGGAAATTGGGAGAAACCCTTGTTAATCGTCTCCGCTATCCGAATAGAAACCAAAAGGCAGGTACTGGCTCGCAGGACAAAGCAAATGAAATTGAGAAACAGGGAGCTGGGAAATATGTTGATATGCTGCCCATTCAGCCTATGATTTTTGGTGTAAAGCTGAGAAACTTCCCTGAAAATATCGGCAGTAATCGGCAGGTAGGTCTAAAATCCAGTTACAGACGAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

337

Amino Acids

37.76

Weight (kDa)

9.1

Isoelectric Point (pI)

58.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 107 - 274 3.5e-46 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 830, 967
AccII CGCG 1 cut(s) 178
AciI CCGC 3 cut(s) 57, 161, 814
AclWI GGATC 1 cut(s) 225
AcsI RAATTY 2 cut(s) 393, 539
AcuI CTGAAG 1 cut(s) 294
AfaI GTAC 1 cut(s) 844
AfiI CCNNNNNNNGG 3 cut(s) 76, 88, 254
AgsI TTSAA 8 cut(s) 206, 349, 400, 428, 494, 539, 707, 755
AjuI GAANNNNNNNTTGG 2 cut(s) 392, 424
AluBI AGCT 5 cut(s) 7, 232, 758, 887, 940
AluI AGCT 5 cut(s) 7, 232, 758, 887, 940
Alw21I GWGCWC 1 cut(s) 554
Alw26I GTCTC 2 cut(s) 51, 815
AlwI GGATC 1 cut(s) 225
AlwNI CAGNNNCTG 3 cut(s) 383, 605, 845
AoxI GGCC 4 cut(s) 67, 90, 112, 121
ApeKI GCWGC 2 cut(s) 671, 907
ApoI RAATTY 2 cut(s) 393, 539
AspLEI GCGC 1 cut(s) 180
AspS9I GGNCC 3 cut(s) 79, 113, 121
AsuC2I CCSGG 1 cut(s) 169
AsuHPI GGTGA 1 cut(s) 518
AvaII GGWCC 1 cut(s) 79
BanII GRGCYC 1 cut(s) 241
Bbv12I GWGCWC 1 cut(s) 554
BbvI GCAGC 2 cut(s) 658, 894
BccI CCATC 3 cut(s) 146, 252, 332
BcnI CCSGG 1 cut(s) 169
BcoDI GTCTC 2 cut(s) 51, 815
BfaI CTAG 2 cut(s) 282, 555
BfmI CTRYAG 2 cut(s) 500, 516
BfuAI ACCTGC 2 cut(s) 830, 967
BglI GCCNNNNNGGC 1 cut(s) 120
BisI GCNGC 3 cut(s) 57, 672, 908
BlsI GCNGC 3 cut(s) 58, 673, 909
Bme1390I CCNGG 1 cut(s) 169
Bme18I GGWCC 1 cut(s) 79
BmgT120I GGNCC 3 cut(s) 79, 113, 121
BmiI GGNNCC 1 cut(s) 240
BmrFI CCNGG 1 cut(s) 169
BmrI ACTGGG 1 cut(s) 324
BmsI GCATC 1 cut(s) 364
BmuI ACTGGG 1 cut(s) 324
BpuMI CCSGG 1 cut(s) 169
BsaBI GATNNNNATC 1 cut(s) 627
BsaJI CCNNGG 3 cut(s) 167, 253, 408
Bsc4I CCNNNNNNNGG 3 cut(s) 76, 88, 254
Bse118I RCCGGY 1 cut(s) 65
Bse1I ACTGG 5 cut(s) 43, 319, 577, 850, 993
Bse8I GATNNNNATC 1 cut(s) 627
BseDI CCNNGG 3 cut(s) 167, 253, 408
BseGI GGATG 1 cut(s) 263
BseJI GATNNNNATC 1 cut(s) 627
BseLI CCNNNNNNNGG 3 cut(s) 76, 88, 254
BseMII CTCAG 2 cut(s) 62, 932
BseNI ACTGG 5 cut(s) 43, 319, 577, 850, 993
BseXI GCAGC 2 cut(s) 658, 894
BseYI CCCAGC 1 cut(s) 887
Bsh1236I CGCG 1 cut(s) 178
BshFI GGCC 4 cut(s) 69, 92, 114, 123
BsiHKAI GWGCWC 1 cut(s) 554
BsiSI CCGG 4 cut(s) 66, 89, 169, 191
BslI CCNNNNNNNGG 3 cut(s) 76, 88, 254
BsmAI GTCTC 2 cut(s) 51, 815
BsmBI CGTCTC 1 cut(s) 815
BsmI GAATGC 3 cut(s) 160, 172, 406
BsnI GGCC 4 cut(s) 69, 92, 114, 123
Bsp1286I GDGCHC 2 cut(s) 241, 554
Bsp143I GATC 1 cut(s) 217
BspACI CCGC 3 cut(s) 57, 161, 814
BspANI GGCC 4 cut(s) 69, 92, 114, 123
BspCNI CTCAG 2 cut(s) 61, 933
BspFNI CGCG 1 cut(s) 178
BspLI GGNNCC 1 cut(s) 240
BspMI ACCTGC 2 cut(s) 830, 967
BspPI GGATC 1 cut(s) 225
BspQI GCTCTTC 1 cut(s) 270
BsrFI RCCGGY 1 cut(s) 65
BsrI ACTGG 5 cut(s) 43, 319, 577, 850, 993
BssAI RCCGGY 1 cut(s) 65
BssECI CCNNGG 3 cut(s) 167, 253, 408
BssMI GATC 1 cut(s) 217
BssT1I CCWWGG 2 cut(s) 253, 408
Bst6I CTCTTC 1 cut(s) 270
BstC8I GCNNGC 3 cut(s) 67, 230, 851
BstDEI CTNAG 3 cut(s) 48, 185, 941
BstF5I GGATG 1 cut(s) 263
BstFNI CGCG 1 cut(s) 178
BstHHI GCGC 1 cut(s) 180
BstKTI GATC 1 cut(s) 220
BstMAI GTCTC 2 cut(s) 51, 815
BstMBI GATC 1 cut(s) 217
BstMWI GCNNNNNNNGC 5 cut(s) 62, 120, 169, 652, 916
BstSCI CCNGG 1 cut(s) 167
BstSFI CTRYAG 2 cut(s) 500, 516
BstUI CGCG 1 cut(s) 178
BstV1I GCAGC 2 cut(s) 658, 894
BsuRI GGCC 4 cut(s) 69, 92, 114, 123
BtsCI GGATG 1 cut(s) 263
BveI ACCTGC 2 cut(s) 830, 967
Cac8I GCNNGC 3 cut(s) 67, 230, 851
CaiI CAGNNNCTG 3 cut(s) 383, 605, 845
CfoI GCGC 1 cut(s) 180
Cfr10I RCCGGY 1 cut(s) 65
Cfr13I GGNCC 3 cut(s) 79, 113, 121
Csp6I GTAC 1 cut(s) 843
CviAII CATG 1 cut(s) 611
CviQI GTAC 1 cut(s) 843
DdeI CTNAG 3 cut(s) 48, 185, 941
DpnI GATC 1 cut(s) 219
DpnII GATC 1 cut(s) 217
Eam1104I CTCTTC 1 cut(s) 270
EarI CTCTTC 1 cut(s) 270
Eco130I CCWWGG 2 cut(s) 253, 408
Eco24I GRGCYC 1 cut(s) 241
Eco47I GGWCC 1 cut(s) 79
Eco57I CTGAAG 1 cut(s) 294
EcoT14I CCWWGG 2 cut(s) 253, 408
EcoT38I GRGCYC 1 cut(s) 241
ErhI CCWWGG 2 cut(s) 253, 408
Esp3I CGTCTC 1 cut(s) 815
FaeI CATG 1 cut(s) 614
FalI AAGNNNNNCTT 2 cut(s) 178, 210
FatI CATG 1 cut(s) 610
FauI CCCGC 1 cut(s) 168
Fnu4HI GCNGC 3 cut(s) 57, 672, 908
FokI GGATG 1 cut(s) 270
FriOI GRGCYC 1 cut(s) 241
Fsp4HI GCNGC 3 cut(s) 57, 672, 908
FspBI CTAG 2 cut(s) 282, 555
GlaI GCGC 1 cut(s) 179
GluI GCNGC 3 cut(s) 57, 672, 908
GsaI CCCAGC 1 cut(s) 891
HaeIII GGCC 4 cut(s) 69, 92, 114, 123
HapII CCGG 4 cut(s) 66, 89, 169, 191
HhaI GCGC 1 cut(s) 180
Hin1II CATG 1 cut(s) 614
Hin6I GCGC 1 cut(s) 178
HinP1I GCGC 1 cut(s) 178
HincII GTYRAC 1 cut(s) 748
HindII GTYRAC 1 cut(s) 748
HindIII AAGCTT 3 cut(s) 5, 230, 756
HinfI GANTC 2 cut(s) 308, 449
HpaII CCGG 4 cut(s) 66, 89, 169, 191
HphI GGTGA 1 cut(s) 518
Hpy166II GTNNAC 1 cut(s) 748
Hpy188I TCNGA 4 cut(s) 51, 148, 622, 822
Hpy188III TCNNGA 3 cut(s) 272, 632, 781
Hpy8I GTNNAC 1 cut(s) 748
Hpy99I CGWCG 1 cut(s) 301
HpyAV CCTTC 4 cut(s) 103, 144, 191, 539
HpyCH4IV ACGT 2 cut(s) 639, 736
HpyCH4V TGCA 2 cut(s) 377, 655
HpyF10VI GCNNNNNNNGC 5 cut(s) 62, 120, 169, 652, 916
HpyF3I CTNAG 3 cut(s) 48, 185, 941
HpySE526I ACGT 2 cut(s) 639, 736
Hsp92II CATG 1 cut(s) 614
HspAI GCGC 1 cut(s) 178
KroI GCCGGC 1 cut(s) 65
KroNI GCCGGC 1 cut(s) 67
Kzo9I GATC 1 cut(s) 217
LguI GCTCTTC 1 cut(s) 270
LmnI GCTCC 3 cut(s) 244, 549, 884
Lsp1109I GCAGC 2 cut(s) 658, 894
LweI GCATC 1 cut(s) 364
MaeI CTAG 2 cut(s) 282, 555
MaeII ACGT 2 cut(s) 639, 736
MaeIII GTNAC 2 cut(s) 482, 995
MalI GATC 1 cut(s) 219
MboI GATC 1 cut(s) 217
MboII GAAGA 5 cut(s) 133, 194, 201, 287, 609
MhlI GDGCHC 2 cut(s) 241, 554
MluCI AATT 4 cut(s) 393, 539, 785, 870
MnlI CCTC 7 cut(s) 92, 116, 139, 335, 457, 726, 783
MroNI GCCGGC 1 cut(s) 65
MseI TTAA 3 cut(s) 432, 522, 804
MspI CCGG 4 cut(s) 66, 89, 169, 191
MspR9I CCNGG 1 cut(s) 169
Mva1269I GAATGC 3 cut(s) 160, 172, 406
MvnI CGCG 1 cut(s) 178
MwoI GCNNNNNNNGC 5 cut(s) 62, 120, 169, 652, 916
NaeI GCCGGC 1 cut(s) 67
NciI CCSGG 1 cut(s) 169
NdeII GATC 1 cut(s) 217
NgoMIV GCCGGC 1 cut(s) 65
NlaIII CATG 1 cut(s) 614
NlaIV GGNNCC 1 cut(s) 240
NmuCI GTSAC 1 cut(s) 482
PciSI GCTCTTC 1 cut(s) 270
PctI GAATGC 3 cut(s) 160, 172, 406
PdiI GCCGGC 1 cut(s) 67
PfeI GAWTC 2 cut(s) 308, 449
PkrI GCNGC 3 cut(s) 58, 673, 909
PspFI CCCAGC 1 cut(s) 887
PspN4I GGNNCC 1 cut(s) 240
PspPI GGNCC 3 cut(s) 79, 113, 121
PstNI CAGNNNCTG 3 cut(s) 383, 605, 845
RsaI GTAC 1 cut(s) 844
RsaNI GTAC 1 cut(s) 843
SapI GCTCTTC 1 cut(s) 270
SaqAI TTAA 3 cut(s) 432, 522, 804
SatI GCNGC 3 cut(s) 57, 672, 908
Sau3AI GATC 1 cut(s) 217
Sau96I GGNCC 3 cut(s) 79, 113, 121
ScrFI CCNGG 1 cut(s) 169
SduI GDGCHC 2 cut(s) 241, 554
SfaNI GCATC 1 cut(s) 364
SfcI CTRYAG 2 cut(s) 500, 516
SfiI GGCCNNNNNGGCC 1 cut(s) 120
SinI GGWCC 1 cut(s) 79
Sse9I AATT 4 cut(s) 393, 539, 785, 870
SsiI CCGC 3 cut(s) 57, 161, 814
SspMI CTAG 2 cut(s) 282, 555
StyD4I CCNGG 1 cut(s) 167
StyI CCWWGG 2 cut(s) 253, 408
TaiI ACGT 2 cut(s) 642, 739
TaqI TCGA 1 cut(s) 452
TasI AATT 4 cut(s) 393, 539, 785, 870
TauI GCSGC 1 cut(s) 59
TfiI GAWTC 2 cut(s) 308, 449
Tru1I TTAA 3 cut(s) 432, 522, 804
Tru9I TTAA 3 cut(s) 432, 522, 804
TseFI GTSAC 1 cut(s) 482
TseI GCWGC 2 cut(s) 671, 907
Tsp45I GTSAC 1 cut(s) 482
TspDTI ATGAA 3 cut(s) 17, 599, 882
TspGWI ACGGA 2 cut(s) 231, 595
VpaK11BI GGWCC 1 cut(s) 79
XapI RAATTY 2 cut(s) 393, 539
XspI CTAG 2 cut(s) 282, 555
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.