AT4G25150

acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0010270
Physical Location & Seq
Reverse (-)
12901534 .. 12903151
1618 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G25150.1

Sequence Viewer

Length: 783 bp
ATGCGGATTCTCGTGAATCTCATTCTCTTCTCCTTGATTCCTCTGGCGTTCTCCAATGAAAACTCCAGCTCGTATCTCATTGCGCGACCATTGATCTTCGAAACCCAGCTCAAGAACATAAACGACAATGTCAATCTACATTGCACGAGCTGGAGATTCGCGGCGGAGACAAACAATCTCGCTCCATGGAAAACCATTCCAGCTGAATGCGCCGATTACGTGAAAGATTACCTGATGGGTGAAGGTTACGTCGTCGATGTGGAGAGAGTCTCCGAAGAAGCTAAGGTTTACGCGAGCAGCTTCGAATCTAACGGTGACGGGAAAGACATTTGGATTTTCGATATTGATGAGACGTTATTGTCGAATCTTCCTTATTACATGGAACACGGTTGTGGGTTGGAAGTTTTTGATCATTCAAAGTTTGATATGTGGGTAGAGAAAGGGATAGCACCTGCTATAGCACCAAGCTTGAAGCTTTACCAAAAGGTTATACATTTGGGGTACAAAGTTATCTTGCTTACAGGGCGTAGAGAGAACCACCGGGTTATCACTGTCGAAAACCTGCGCAATGCCGGTTTCCATAACTGGGACAAGCTTATTCTGAGATCGTTGGATGATCGGAACAAAACAGCGACCATGTACAAGTCAGAGAAGAGAGAGGAGATGGTGAAAGAAGGGTATAGGATCAGAGGCAATTCAGGTGACCAATGGAGTGATTTGTTGGGTTCTGCAATGTCAGAAAGATCCTTCAAACTTCCAAATCCAATGTATTATATCCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Acid_phosphat_B

Protein Analysis

260

Amino Acids

29.96

Weight (kDa)

5.75

Isoelectric Point (pI)

38.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Acid_phosphat_B PF03767 35 - 260 9.1e-93 HAD superfamily, subfamily IIIB (Acid phosphatase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010270)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G25150 AT5G51260 AT5G51260
fragaria_vesca FvH4_7g27010 FvH4_7g27010 FvH4_7g27010
malus_domestica MD01G1177700.v1.1 MD07G1249500.v1.1
prunus_persica Prupe.2G273200_v2.0.a1
pyrus_communis pycom01g18850 pycom07g22280
rosa_chinensis RchiOBHm_Chr1g0373811
rosa_laevigata RLG00000026804
rosa_multiflora Rmu_ssc0000294.1_g000001
rosa_roxburghii Rroxscaffold_4G00283990
rosa_rugosa Rorug01G0379500
rosa_samantha Rh1AG388900 Rh1BG353100 Rh1CG366300 Rh1DG384000
rosa_wichuraiana Rw1G034630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 460
AasI GACNNNNNNGTC 1 cut(s) 358
Acc16I TGCGCA 1 cut(s) 566
Acc36I ACCTGC 2 cut(s) 460, 570
AccII CGCG 3 cut(s) 85, 161, 293
AciI CCGC 3 cut(s) 4, 161, 164
AclWI GGATC 2 cut(s) 692, 738
AfaI GTAC 2 cut(s) 503, 641
AfiI CCNNNNNNNGG 1 cut(s) 586
AgsI TTSAA 3 cut(s) 417, 472, 751
AleI CACNNNNGTG 1 cut(s) 390
AluBI AGCT 9 cut(s) 69, 109, 150, 203, 281, 300, 468, 475, 595
AluI AGCT 9 cut(s) 69, 109, 150, 203, 281, 300, 468, 475, 595
Alw26I GTCTC 3 cut(s) 161, 274, 344
AlwI GGATC 2 cut(s) 692, 738
ApeKI GCWGC 1 cut(s) 297
AspLEI GCGC 3 cut(s) 85, 212, 567
AsuC2I CCSGG 1 cut(s) 542
AsuHPI GGTGA 4 cut(s) 251, 326, 679, 713
AsuII TTCGAA 2 cut(s) 99, 303
BauI CACGAG 2 cut(s) 11, 145
BbvI GCAGC 1 cut(s) 309
BccI CCATC 2 cut(s) 229, 658
BclI TGATCA 1 cut(s) 409
BcnI CCSGG 1 cut(s) 542
BcoDI GTCTC 3 cut(s) 161, 274, 344
BfmI CTRYAG 1 cut(s) 456
BfuAI ACCTGC 2 cut(s) 460, 570
BisI GCNGC 2 cut(s) 162, 298
BlsI GCNGC 2 cut(s) 163, 299
Bme1390I CCNGG 1 cut(s) 542
BmrFI CCNGG 1 cut(s) 542
BmrI ACTGGG 1 cut(s) 595
BmuI ACTGGG 1 cut(s) 595
BplI GAGNNNNNCTC 2 cut(s) 254, 286
BpmI CTGGAG 2 cut(s) 49, 172
Bpu10I CCTNAGC 1 cut(s) 282
Bpu14I TTCGAA 2 cut(s) 99, 303
BpuEI CTTGAG 1 cut(s) 95
BpuMI CCSGG 1 cut(s) 542
BsaAI YACGTR 1 cut(s) 220
BsaJI CCNNGG 1 cut(s) 185
Bsc4I CCNNNNNNNGG 1 cut(s) 586
Bse118I RCCGGY 1 cut(s) 572
Bse1I ACTGG 1 cut(s) 590
Bse3DI GCAATG 4 cut(s) 78, 139, 574, 738
BseDI CCNNGG 1 cut(s) 185
BseGI GGATG 1 cut(s) 619
BseLI CCNNNNNNNGG 1 cut(s) 586
BseMI GCAATG 4 cut(s) 78, 139, 574, 738
BseMII CTCAG 1 cut(s) 593
BseNI ACTGG 1 cut(s) 590
BseRI GAGGAG 1 cut(s) 674
BseXI GCAGC 1 cut(s) 309
BseYI CCCAGC 1 cut(s) 105
Bsh1236I CGCG 3 cut(s) 85, 161, 293
BsiSI CCGG 2 cut(s) 541, 573
BslFI GGGAC 1 cut(s) 602
BslI CCNNNNNNNGG 1 cut(s) 586
BsmAI GTCTC 3 cut(s) 161, 274, 344
BsmBI CGTCTC 1 cut(s) 344
BsmFI GGGAC 1 cut(s) 602
BsmI GAATGC 1 cut(s) 212
Bsp119I TTCGAA 2 cut(s) 99, 303
Bsp1407I TGTACA 1 cut(s) 639
Bsp143I GATC 6 cut(s) 93, 409, 605, 616, 684, 743
Bsp19I CCATGG 1 cut(s) 185
BspACI CCGC 3 cut(s) 4, 161, 164
BspCNI CTCAG 1 cut(s) 594
BspFNI CGCG 3 cut(s) 85, 161, 293
BspMI ACCTGC 2 cut(s) 460, 570
BspPI GGATC 2 cut(s) 692, 738
BspT104I TTCGAA 2 cut(s) 99, 303
BsrDI GCAATG 4 cut(s) 78, 139, 574, 738
BsrFI RCCGGY 1 cut(s) 572
BsrGI TGTACA 1 cut(s) 639
BsrI ACTGG 1 cut(s) 590
BssAI RCCGGY 1 cut(s) 572
BssECI CCNNGG 1 cut(s) 185
BssMI GATC 6 cut(s) 93, 409, 605, 616, 684, 743
BssSI CACGAG 2 cut(s) 11, 145
BssT1I CCWWGG 1 cut(s) 185
Bst2BI CACGAG 2 cut(s) 11, 145
Bst4CI ACNGT 3 cut(s) 314, 389, 553
Bst6I CTCTTC 2 cut(s) 32, 647
BstAUI TGTACA 1 cut(s) 639
BstBAI YACGTR 1 cut(s) 220
BstBI TTCGAA 2 cut(s) 99, 303
BstC8I GCNNGC 1 cut(s) 295
BstDEI CTNAG 2 cut(s) 282, 602
BstDSI CCRYGG 1 cut(s) 185
BstEII GGTNACC 1 cut(s) 701
BstF5I GGATG 1 cut(s) 619
BstFNI CGCG 3 cut(s) 85, 161, 293
BstHHI GCGC 3 cut(s) 85, 212, 567
BstKTI GATC 6 cut(s) 96, 412, 608, 619, 687, 746
BstMAI GTCTC 3 cut(s) 161, 274, 344
BstMBI GATC 6 cut(s) 93, 409, 605, 616, 684, 743
BstMWI GCNNNNNNNGC 2 cut(s) 209, 523
BstPI GGTNACC 1 cut(s) 701
BstSCI CCNGG 1 cut(s) 540
BstSFI CTRYAG 1 cut(s) 456
BstUI CGCG 3 cut(s) 85, 161, 293
BstV1I GCAGC 1 cut(s) 309
BstX2I RGATCY 1 cut(s) 743
BstYI RGATCY 1 cut(s) 743
BtgI CCRYGG 1 cut(s) 185
BtsCI GGATG 1 cut(s) 619
BtsIMutI CAGTG 1 cut(s) 549
BveI ACCTGC 2 cut(s) 460, 570
Cac8I GCNNGC 1 cut(s) 295
CfoI GCGC 3 cut(s) 85, 212, 567
Cfr10I RCCGGY 1 cut(s) 572
Csp6I GTAC 2 cut(s) 502, 640
CviAII CATG 3 cut(s) 186, 379, 637
CviJI RGCY 9 cut(s) 69, 109, 150, 203, 281, 300, 468, 475, 595
CviKI_1 RGCY 9 cut(s) 69, 109, 150, 203, 281, 300, 468, 475, 595
CviQI GTAC 2 cut(s) 502, 640
DdeI CTNAG 2 cut(s) 282, 602
DpnI GATC 6 cut(s) 95, 411, 607, 618, 686, 745
DpnII GATC 6 cut(s) 93, 409, 605, 616, 684, 743
DrdI GACNNNNNNGTC 1 cut(s) 358
DseDI GACNNNNNNGTC 1 cut(s) 358
Eam1104I CTCTTC 2 cut(s) 32, 647
EarI CTCTTC 2 cut(s) 32, 647
EciI GGCGGA 1 cut(s) 179
Eco130I CCWWGG 1 cut(s) 185
Eco91I GGTNACC 1 cut(s) 701
EcoO65I GGTNACC 1 cut(s) 701
EcoT14I CCWWGG 1 cut(s) 185
ErhI CCWWGG 1 cut(s) 185
Esp3I CGTCTC 1 cut(s) 344
FaeI CATG 3 cut(s) 189, 382, 640
FaqI GGGAC 1 cut(s) 602
FatI CATG 3 cut(s) 185, 378, 636
FbaI TGATCA 1 cut(s) 409
Fnu4HI GCNGC 2 cut(s) 162, 298
FokI GGATG 1 cut(s) 626
Fsp4HI GCNGC 2 cut(s) 162, 298
FspI TGCGCA 1 cut(s) 566
GlaI GCGC 3 cut(s) 84, 211, 566
GluI GCNGC 2 cut(s) 162, 298
GsaI CCCAGC 1 cut(s) 109
GsuI CTGGAG 2 cut(s) 49, 172
HapII CCGG 2 cut(s) 541, 573
HhaI GCGC 3 cut(s) 85, 212, 567
Hin1II CATG 3 cut(s) 189, 382, 640
Hin6I GCGC 3 cut(s) 83, 210, 565
HinP1I GCGC 3 cut(s) 83, 210, 565
HindIII AAGCTT 3 cut(s) 466, 473, 593
HinfI GANTC 7 cut(s) 7, 16, 37, 156, 267, 305, 364
HpaII CCGG 2 cut(s) 541, 573
HphI GGTGA 4 cut(s) 251, 326, 679, 713
Hpy166II GTNNAC 1 cut(s) 289
Hpy188I TCNGA 6 cut(s) 274, 603, 621, 649, 689, 739
Hpy188III TCNNGA 2 cut(s) 13, 112
Hpy8I GTNNAC 1 cut(s) 289
Hpy99I CGWCG 2 cut(s) 254, 257
HpyAV CCTTC 3 cut(s) 236, 668, 757
HpyCH4III ACNGT 3 cut(s) 314, 389, 553
HpyCH4IV ACGT 3 cut(s) 219, 249, 353
HpyCH4V TGCA 2 cut(s) 144, 731
HpyF10VI GCNNNNNNNGC 2 cut(s) 209, 523
HpyF3I CTNAG 2 cut(s) 282, 602
HpySE526I ACGT 3 cut(s) 219, 249, 353
Hsp92II CATG 3 cut(s) 189, 382, 640
HspAI GCGC 3 cut(s) 83, 210, 565
Ksp22I TGATCA 1 cut(s) 409
Kzo9I GATC 6 cut(s) 93, 409, 605, 616, 684, 743
LmnI GCTCC 1 cut(s) 187
Lsp1109I GCAGC 1 cut(s) 309
MaeII ACGT 3 cut(s) 219, 249, 353
MaeIII GTNAC 3 cut(s) 245, 314, 701
MalI GATC 6 cut(s) 95, 411, 607, 618, 686, 745
MboI GATC 6 cut(s) 93, 409, 605, 616, 684, 743
MboII GAAGA 5 cut(s) 19, 88, 287, 359, 664
MflI RGATCY 1 cut(s) 743
MluCI AATT 1 cut(s) 694
MlyI GAGTC 1 cut(s) 276
MmeI TCCRAC 2 cut(s) 378, 591
MnlI CCTC 3 cut(s) 51, 652, 683
MslI CAYNNNNRTG 1 cut(s) 390
MspA1I CMGCKG 1 cut(s) 203
MspI CCGG 2 cut(s) 541, 573
MspR9I CCNGG 1 cut(s) 542
Mva1269I GAATGC 1 cut(s) 212
MvnI CGCG 3 cut(s) 85, 161, 293
MwoI GCNNNNNNNGC 2 cut(s) 209, 523
NciI CCSGG 1 cut(s) 542
NcoI CCATGG 1 cut(s) 185
NdeII GATC 6 cut(s) 93, 409, 605, 616, 684, 743
NlaIII CATG 3 cut(s) 189, 382, 640
NmuCI GTSAC 2 cut(s) 314, 701
NsbI TGCGCA 1 cut(s) 566
NspV TTCGAA 2 cut(s) 99, 303
OliI CACNNNNGTG 1 cut(s) 390
PaqCI CACCTGC 1 cut(s) 460
PcsI WCGNNNNNNNCGW 1 cut(s) 359
PctI GAATGC 1 cut(s) 212
PfeI GAWTC 6 cut(s) 7, 16, 37, 156, 305, 364
PflFI GACNNNGTC 1 cut(s) 128
PkrI GCNGC 2 cut(s) 163, 299
PleI GAGTC 1 cut(s) 275
PpsI GAGTC 1 cut(s) 275
Ppu21I YACGTR 1 cut(s) 220
PspEI GGTNACC 1 cut(s) 701
PspFI CCCAGC 1 cut(s) 105
PsuI RGATCY 1 cut(s) 743
PsyI GACNNNGTC 1 cut(s) 128
PvuII CAGCTG 1 cut(s) 203
RsaI GTAC 2 cut(s) 503, 641
RsaNI GTAC 2 cut(s) 502, 640
RseI CAYNNNNRTG 1 cut(s) 390
SatI GCNGC 2 cut(s) 162, 298
Sau3AI GATC 6 cut(s) 93, 409, 605, 616, 684, 743
SchI GAGTC 1 cut(s) 276
ScrFI CCNGG 1 cut(s) 542
SfcI CTRYAG 1 cut(s) 456
SfuI TTCGAA 2 cut(s) 99, 303
SmiMI CAYNNNNRTG 1 cut(s) 390
SmlI CTYRAG 1 cut(s) 110
SmoI CTYRAG 1 cut(s) 110
Sse9I AATT 1 cut(s) 694
SsiI CCGC 3 cut(s) 4, 161, 164
StyD4I CCNGG 1 cut(s) 540
StyI CCWWGG 1 cut(s) 185
TaaI ACNGT 3 cut(s) 314, 389, 553
TaiI ACGT 3 cut(s) 222, 252, 356
TaqI TCGA 6 cut(s) 99, 255, 303, 339, 362, 555
TasI AATT 1 cut(s) 694
TatI WGTACW 1 cut(s) 639
TauI GCSGC 1 cut(s) 164
TfiI GAWTC 6 cut(s) 7, 16, 37, 156, 305, 364
TscAI CASTG 1 cut(s) 556
TseFI GTSAC 2 cut(s) 314, 701
TseI GCWGC 1 cut(s) 297
Tsp45I GTSAC 2 cut(s) 314, 701
TspDTI ATGAA 1 cut(s) 72
TspRI CASTG 1 cut(s) 556
Tth111I GACNNNGTC 1 cut(s) 128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.