MD01G1177700.v1.1

acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0010270
Physical Location & Seq
Forward (+)
27821997 .. 27824983
2987 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1177700.v1.1.491

Sequence Viewer

Length: 801 bp
ATGAACTTTTTGAAAATTTTCCTTCTTTTTTCCCTGCTTCCGGTTGCGTTTTCTCACGAGACCTTCGACTCCCGCCTCCTGCCGCGGCCGTTGATCCTCGAATACCCGGACATCGCCGAGACCCACTTCAATGATTTGGAGAATGAGCTCAGATTACACTGCGACAGCTGGAGATTTTCTGTAGAGGCAAACAATGTCAATCCCTGGAAAACAATCCCGGAGGAGTGTGCCGGGTATGTCAAGGATTACTTGACGGGTCGGGCTTACGCATACGATCTCGAAAGGGTGTCTAAGGAGGCTGGGGTTTATGCCAAGAGTGTTGAATTGAATGGCGACGGGAAGGATGTGTGGATTTTCGACATTGATGACACTTTGCTTTCCAACCTTCCCTATTATGCTGACCATGGTTATGGCTTGGAGGTGTTTGACACGGTGGAGTTTGATAAGTGGGTTGAGAAGGCCATGGCACCTGCTATAAAGTCCAGCTTGAAACTCTATGAAGAGGTCTTGAGTTTGGGATTTAAGGTTTTCTTGCTCACGGGGCGCACCGAAGGGAAAAGGAAGGTTACTGTTGAGAATCTGAACAATGCAGGATTCCAAGAATGGCATAAGCTTATTTTGAGGTCCGCTGATGACCACGAGAAATTGGCGATAATTTACAAGTCCGAAAAGAGGAGTGAGATGGAAAAGGAGGGATACAGACTACTTGGGAATTCCGGAGACCAGTGGAGTGATTTACTTGGTACCTCAGTCGGCCTCCGCTCGTTCAAGCTTCCAAATCCTATGTATTACATTCCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Acid_phosphat_B

Protein Analysis

267

Amino Acids

30.67

Weight (kDa)

5.11

Isoelectric Point (pI)

32.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Acid_phosphat_B PF03767 40 - 266 2.6e-88 HAD superfamily, subfamily IIIB (Acid phosphatase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010270)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G25150 AT5G51260 AT5G51260
fragaria_vesca FvH4_7g27010 FvH4_7g27010 FvH4_7g27010
malus_domestica MD01G1177700.v1.1 MD07G1249500.v1.1
prunus_persica Prupe.2G273200_v2.0.a1
pyrus_communis pycom01g18850 pycom07g22280
rosa_chinensis RchiOBHm_Chr1g0373811
rosa_laevigata RLG00000026804
rosa_multiflora Rmu_ssc0000294.1_g000001
rosa_roxburghii Rroxscaffold_4G00283990
rosa_rugosa Rorug01G0379500
rosa_samantha Rh1AG388900 Rh1BG353100 Rh1CG366300 Rh1DG384000
rosa_wichuraiana Rw1G034630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 478
Acc36I ACCTGC 1 cut(s) 478
Acc65I GGTACC 1 cut(s) 743
AccB1I GGYRCC 2 cut(s) 466, 743
AccBSI CCGCTC 1 cut(s) 762
AccII CGCG 1 cut(s) 85
AccIII TCCGGA 1 cut(s) 716
AciI CCGC 5 cut(s) 73, 83, 85, 627, 760
AclWI GGATC 1 cut(s) 88
AcoI YGGCCR 1 cut(s) 86
AcsI RAATTY 2 cut(s) 15, 712
AfaI GTAC 1 cut(s) 745
AfiI CCNNNNNNNGG 2 cut(s) 40, 672
AgsI TTSAA 6 cut(s) 13, 130, 323, 328, 490, 769
AjnI CCWGG 1 cut(s) 203
AluBI AGCT 5 cut(s) 148, 168, 486, 613, 772
AluI AGCT 5 cut(s) 148, 168, 486, 613, 772
Alw21I GWGCWC 1 cut(s) 150
Alw26I GTCTC 3 cut(s) 53, 113, 714
AlwI GGATC 1 cut(s) 88
Aor13HI TCCGGA 1 cut(s) 716
AoxI GGCC 3 cut(s) 86, 459, 754
ApoI RAATTY 2 cut(s) 15, 712
Asp700I GAANNNNTTC 1 cut(s) 17
Asp718I GGTACC 1 cut(s) 743
AspLEI GCGC 1 cut(s) 546
AspS9I GGNCC 1 cut(s) 624
AsuC2I CCSGG 3 cut(s) 107, 218, 232
AvaII GGWCC 1 cut(s) 624
BaeI ACNNNNGTAYC 2 cut(s) 735, 768
BanI GGYRCC 2 cut(s) 466, 743
BanII GRGCYC 1 cut(s) 150
BauI CACGAG 2 cut(s) 56, 638
Bbv12I GWGCWC 1 cut(s) 150
BccI CCATC 1 cut(s) 676
BceAI ACGGC 1 cut(s) 73
BciT130I CCWGG 1 cut(s) 205
BciVI GTATCC 1 cut(s) 689
BcnI CCSGG 3 cut(s) 107, 218, 232
BcoDI GTCTC 3 cut(s) 53, 113, 714
BfmI CTRYAG 1 cut(s) 180
BfuAI ACCTGC 1 cut(s) 478
BfuI GTATCC 1 cut(s) 689
BisI GCNGC 2 cut(s) 83, 86
BlsI GCNGC 2 cut(s) 84, 87
Bme1390I CCNGG 4 cut(s) 107, 205, 218, 232
Bme18I GGWCC 1 cut(s) 624
BmgT120I GGNCC 1 cut(s) 624
BmiI GGNNCC 2 cut(s) 468, 745
BmrFI CCNGG 4 cut(s) 107, 205, 218, 232
BpmI CTGGAG 1 cut(s) 190
BpuEI CTTGAG 1 cut(s) 529
BpuMI CCSGG 3 cut(s) 107, 218, 232
BsaI GGTCTC 3 cut(s) 53, 113, 714
BsaJI CCNNGG 4 cut(s) 83, 203, 403, 462
BsaWI WCCGGW 2 cut(s) 40, 716
Bsc4I CCNNNNNNNGG 2 cut(s) 40, 672
Bse1I ACTGG 1 cut(s) 724
BseAI TCCGGA 1 cut(s) 716
BseBI CCWGG 1 cut(s) 205
BseDI CCNNGG 4 cut(s) 83, 203, 403, 462
BseGI GGATG 1 cut(s) 349
BseLI CCNNNNNNNGG 2 cut(s) 40, 672
BseMII CTCAG 2 cut(s) 163, 762
BseNI ACTGG 1 cut(s) 724
BseRI GAGGAG 2 cut(s) 236, 688
BseX3I CGGCCG 1 cut(s) 86
BseYI CCCAGC 1 cut(s) 299
Bsh1236I CGCG 1 cut(s) 85
Bsh1285I CGRYCG 1 cut(s) 89
BshFI GGCC 3 cut(s) 88, 461, 756
BshNI GGYRCC 2 cut(s) 466, 743
BsiEI CGRYCG 1 cut(s) 89
BsiHKAI GWGCWC 1 cut(s) 150
BsiSI CCGG 5 cut(s) 41, 107, 218, 231, 717
BslI CCNNNNNNNGG 2 cut(s) 40, 672
BsmAI GTCTC 3 cut(s) 53, 113, 714
BsnI GGCC 3 cut(s) 88, 461, 756
Bso31I GGTCTC 3 cut(s) 53, 113, 714
Bsp1286I GDGCHC 1 cut(s) 150
Bsp13I TCCGGA 1 cut(s) 716
Bsp143I GATC 2 cut(s) 93, 274
Bsp19I CCATGG 2 cut(s) 403, 462
BspACI CCGC 5 cut(s) 73, 83, 85, 627, 760
BspANI GGCC 3 cut(s) 88, 461, 756
BspCNI CTCAG 2 cut(s) 162, 761
BspEI TCCGGA 1 cut(s) 716
BspFNI CGCG 1 cut(s) 85
BspLI GGNNCC 2 cut(s) 468, 745
BspMI ACCTGC 1 cut(s) 478
BspPI GGATC 1 cut(s) 88
BspT107I GGYRCC 2 cut(s) 466, 743
BspTNI GGTCTC 3 cut(s) 53, 113, 714
BsrBI CCGCTC 1 cut(s) 762
BsrI ACTGG 1 cut(s) 724
BssECI CCNNGG 4 cut(s) 83, 203, 403, 462
BssMI GATC 2 cut(s) 93, 274
BssSI CACGAG 2 cut(s) 56, 638
BssT1I CCWWGG 2 cut(s) 403, 462
Bst2BI CACGAG 2 cut(s) 56, 638
Bst2UI CCWGG 1 cut(s) 205
Bst4CI ACNGT 2 cut(s) 433, 571
Bst6I CTCTTC 1 cut(s) 495
BstDEI CTNAG 3 cut(s) 149, 291, 748
BstDSI CCRYGG 3 cut(s) 83, 403, 462
BstF5I GGATG 1 cut(s) 349
BstFNI CGCG 1 cut(s) 85
BstHHI GCGC 1 cut(s) 546
BstKTI GATC 2 cut(s) 96, 277
BstMAI GTCTC 3 cut(s) 53, 113, 714
BstMBI GATC 2 cut(s) 93, 274
BstMCI CGRYCG 1 cut(s) 89
BstMWI GCNNNNNNNGC 1 cut(s) 541
BstNI CCWGG 1 cut(s) 205
BstSCI CCNGG 4 cut(s) 105, 203, 216, 230
BstSFI CTRYAG 1 cut(s) 180
BstUI CGCG 1 cut(s) 85
BstXI CCANNNNNNTGG 1 cut(s) 410
BstZI CGGCCG 1 cut(s) 86
BsuI GTATCC 1 cut(s) 689
BsuRI GGCC 3 cut(s) 88, 461, 756
BtgI CCRYGG 3 cut(s) 83, 403, 462
BtgZI GCGATG 1 cut(s) 97
BtsCI GGATG 1 cut(s) 349
BtsI GCAGTG 1 cut(s) 157
BtsIMutI CAGTG 2 cut(s) 157, 731
BveI ACCTGC 1 cut(s) 478
CfoI GCGC 1 cut(s) 546
Cfr13I GGNCC 1 cut(s) 624
Cfr42I CCGCGG 1 cut(s) 86
Csp6I GTAC 1 cut(s) 744
CviAII CATG 2 cut(s) 404, 463
CviQI GTAC 1 cut(s) 744
DdeI CTNAG 3 cut(s) 149, 291, 748
DpnI GATC 2 cut(s) 95, 276
DpnII GATC 2 cut(s) 93, 274
EaeI YGGCCR 1 cut(s) 86
EagI CGGCCG 1 cut(s) 86
Eam1104I CTCTTC 1 cut(s) 495
EarI CTCTTC 1 cut(s) 495
Ecl136II GAGCTC 1 cut(s) 148
EclXI CGGCCG 1 cut(s) 86
Eco130I CCWWGG 2 cut(s) 403, 462
Eco24I GRGCYC 1 cut(s) 150
Eco31I GGTCTC 3 cut(s) 53, 113, 714
Eco47I GGWCC 1 cut(s) 624
Eco52I CGGCCG 1 cut(s) 86
Eco53kI GAGCTC 1 cut(s) 148
EcoICRI GAGCTC 1 cut(s) 148
EcoRI GAATTC 1 cut(s) 712
EcoRII CCWGG 1 cut(s) 203
EcoT14I CCWWGG 2 cut(s) 403, 462
EcoT38I GRGCYC 1 cut(s) 150
ErhI CCWWGG 2 cut(s) 403, 462
FaeI CATG 2 cut(s) 407, 466
FalI AAGNNNNNCTT 6 cut(s) 233, 265, 470, 502, 515, 547
FatI CATG 2 cut(s) 403, 462
FauI CCCGC 1 cut(s) 80
Fnu4HI GCNGC 2 cut(s) 83, 86
FokI GGATG 1 cut(s) 356
FriOI GRGCYC 1 cut(s) 150
Fsp4HI GCNGC 2 cut(s) 83, 86
GlaI GCGC 1 cut(s) 545
GluI GCNGC 2 cut(s) 83, 86
GsaI CCCAGC 1 cut(s) 303
GsuI CTGGAG 1 cut(s) 190
HaeIII GGCC 3 cut(s) 88, 461, 756
HapII CCGG 5 cut(s) 41, 107, 218, 231, 717
HhaI GCGC 1 cut(s) 546
Hin1II CATG 2 cut(s) 407, 466
Hin6I GCGC 1 cut(s) 544
HinP1I GCGC 1 cut(s) 544
HindIII AAGCTT 2 cut(s) 611, 770
HinfI GANTC 3 cut(s) 68, 577, 594
HpaII CCGG 5 cut(s) 41, 107, 218, 231, 717
Hpy188I TCNGA 3 cut(s) 152, 582, 667
Hpy188III TCNNGA 4 cut(s) 56, 278, 508, 717
Hpy99I CGWCG 1 cut(s) 338
HpyAV CCTTC 7 cut(s) 32, 73, 334, 395, 451, 545, 556
HpyCH4III ACNGT 2 cut(s) 433, 571
HpyCH4V TGCA 1 cut(s) 590
HpyF10VI GCNNNNNNNGC 1 cut(s) 541
HpyF3I CTNAG 3 cut(s) 149, 291, 748
Hsp92II CATG 2 cut(s) 407, 466
HspAI GCGC 1 cut(s) 544
Kpn2I TCCGGA 1 cut(s) 716
KpnI GGTACC 1 cut(s) 747
KspI CCGCGG 1 cut(s) 86
Kzo9I GATC 2 cut(s) 93, 274
MaeIII GTNAC 1 cut(s) 565
MalI GATC 2 cut(s) 95, 276
MbiI CCGCTC 1 cut(s) 762
MboI GATC 2 cut(s) 93, 274
MboII GAAGA 1 cut(s) 512
MhlI GDGCHC 1 cut(s) 150
MluCI AATT 5 cut(s) 15, 323, 644, 654, 712
MlyI GAGTC 1 cut(s) 62
MmeI TCCRAC 1 cut(s) 405
MroI TCCGGA 1 cut(s) 716
MroXI GAANNNNTTC 1 cut(s) 17
MseI TTAA 1 cut(s) 522
MslI CAYNNNNRTG 2 cut(s) 129, 408
MspA1I CMGCKG 3 cut(s) 85, 168, 629
MspI CCGG 5 cut(s) 41, 107, 218, 231, 717
MspR9I CCNGG 4 cut(s) 107, 205, 218, 232
MvaI CCWGG 1 cut(s) 205
MvnI CGCG 1 cut(s) 85
MwoI GCNNNNNNNGC 1 cut(s) 541
NciI CCSGG 3 cut(s) 107, 218, 232
NcoI CCATGG 2 cut(s) 403, 462
NdeII GATC 2 cut(s) 93, 274
NlaIII CATG 2 cut(s) 407, 466
NlaIV GGNNCC 2 cut(s) 468, 745
NmeAIII GCCGAG 1 cut(s) 142
PaqCI CACCTGC 1 cut(s) 478
PcsI WCGNNNNNNNCGW 1 cut(s) 63
PdmI GAANNNNTTC 1 cut(s) 17
PfeI GAWTC 2 cut(s) 577, 594
PfoI TCCNGGA 1 cut(s) 216
PkrI GCNGC 2 cut(s) 84, 87
PleI GAGTC 1 cut(s) 62
PpsI GAGTC 1 cut(s) 62
Psp124BI GAGCTC 1 cut(s) 150
Psp6I CCWGG 1 cut(s) 203
PspFI CCCAGC 1 cut(s) 299
PspGI CCWGG 1 cut(s) 203
PspN4I GGNNCC 2 cut(s) 468, 745
PspPI GGNCC 1 cut(s) 624
PvuII CAGCTG 1 cut(s) 168
RsaI GTAC 1 cut(s) 745
RsaNI GTAC 1 cut(s) 744
RseI CAYNNNNRTG 2 cut(s) 129, 408
SacI GAGCTC 1 cut(s) 150
SacII CCGCGG 1 cut(s) 86
SaqAI TTAA 1 cut(s) 522
SatI GCNGC 2 cut(s) 83, 86
Sau3AI GATC 2 cut(s) 93, 274
Sau96I GGNCC 1 cut(s) 624
SchI GAGTC 1 cut(s) 62
ScrFI CCNGG 4 cut(s) 107, 205, 218, 232
SduI GDGCHC 1 cut(s) 150
SfcI CTRYAG 1 cut(s) 180
Sfr303I CCGCGG 1 cut(s) 86
SgrBI CCGCGG 1 cut(s) 86
SinI GGWCC 1 cut(s) 624
SmiMI CAYNNNNRTG 2 cut(s) 129, 408
SmlI CTYRAG 1 cut(s) 508
SmoI CTYRAG 1 cut(s) 508
Sse9I AATT 5 cut(s) 15, 323, 644, 654, 712
SsiI CCGC 5 cut(s) 73, 83, 85, 627, 760
SstI GAGCTC 1 cut(s) 150
StyD4I CCNGG 4 cut(s) 105, 203, 216, 230
StyI CCWWGG 2 cut(s) 403, 462
TaaI ACNGT 2 cut(s) 433, 571
TaqI TCGA 4 cut(s) 66, 99, 279, 357
TasI AATT 5 cut(s) 15, 323, 644, 654, 712
TauI GCSGC 2 cut(s) 85, 88
TfiI GAWTC 2 cut(s) 577, 594
Tru1I TTAA 1 cut(s) 522
Tru9I TTAA 1 cut(s) 522
TscAI CASTG 2 cut(s) 164, 731
TspDTI ATGAA 2 cut(s) 17, 513
TspGWI ACGGA 1 cut(s) 786
TspRI CASTG 2 cut(s) 164, 731
VpaK11BI GGWCC 1 cut(s) 624
XapI RAATTY 2 cut(s) 15, 712
XmnI GAANNNNTTC 1 cut(s) 17
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.