MD07G1249500.v1.1

acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0010270
Physical Location & Seq
Forward (+)
31836291 .. 31838978
2688 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1249500.v1.1.491

Sequence Viewer

Length: 801 bp
ATGAAGTTTTTGAAAATTTGCCTTCTTTTTTCCCTGCTTTCCGTCGCGTTTTCTCACGGGACCTTCGACTCCCACCTCCTGCCGCGGCCGTTGATCCTTGAATACCAAGAAAACACCGAGACCCAGTTCAAGGAATTGGAGGATGAGCTCGGATTACACTGCGCCAGCTGGAGATTTTCTGTGGAAGCAAACAATGTCAATCCCTGGGAAACAATCCCGGAGGAGTGTGCGGAGTATGTCAAAGATTACTTGACGGGTCGGGCTTACGGGTTCGATCTCGAAAGGGTGTCTAAGGAGGCTGGGGTTTACGCCAAGAGTGTTGAATTGAATGGTGATGGAAAGGATGTGTGGATTTTTGACATTGATGACACGCTGCTCTCTAATCTTCCCTATTATACTGACCATGGTTACGGCTTGGAGGTTTTTGATAATGTGGAGTTTGATAAGTGGGTTGAGAAGGCCATGGCACCTGCCATAAAGTCCAGCTTGAAACTCTATGAAGAGGTCTTGAGTTTGGGTTTTAAGGTTTTCTTGCTCACGGGGCGCACCGAAGGGAAAAGGAAGGTTACTGTTGAGAATCTGAACAATGCAGGGTTTCGAGATTGGCATAAGCTTATTTTGAGGTCCGCTGATGACCGCGAGAAACTGGCGACGATTTACAAGTCAGAGAAGAGGAGTGAGATGGAAAAGGAGGGATATAGAATACTTGGGAATTCCGGAGACCAGTGGAGTGATTTACTGGGTACCTCAGTCTCCCTCCGCTCGTTCAAGCTTCCGAATCCTATGTATTATATTCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Acid_phosphat_B

Protein Analysis

267

Amino Acids

30.63

Weight (kDa)

5.07

Isoelectric Point (pI)

28.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Acid_phosphat_B PF03767 39 - 266 2.7e-88 HAD superfamily, subfamily IIIB (Acid phosphatase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010270)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G25150 AT5G51260 AT5G51260
fragaria_vesca FvH4_7g27010 FvH4_7g27010 FvH4_7g27010
malus_domestica MD01G1177700.v1.1 MD07G1249500.v1.1
prunus_persica Prupe.2G273200_v2.0.a1
pyrus_communis pycom01g18850 pycom07g22280
rosa_chinensis RchiOBHm_Chr1g0373811
rosa_laevigata RLG00000026804
rosa_multiflora Rmu_ssc0000294.1_g000001
rosa_roxburghii Rroxscaffold_4G00283990
rosa_rugosa Rorug01G0379500
rosa_samantha Rh1AG388900 Rh1BG353100 Rh1CG366300 Rh1DG384000
rosa_wichuraiana Rw1G034630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 478
Acc36I ACCTGC 1 cut(s) 478
Acc65I GGTACC 1 cut(s) 743
AccB1I GGYRCC 2 cut(s) 466, 743
AccBSI CCGCTC 1 cut(s) 762
AccII CGCG 3 cut(s) 47, 85, 639
AccIII TCCGGA 1 cut(s) 716
AciI CCGC 6 cut(s) 83, 85, 230, 627, 637, 760
AclWI GGATC 1 cut(s) 88
AcoI YGGCCR 1 cut(s) 86
AcsI RAATTY 2 cut(s) 15, 712
AfaI GTAC 1 cut(s) 745
AfiI CCNNNNNNNGG 1 cut(s) 130
AgsI TTSAA 7 cut(s) 13, 101, 130, 323, 328, 490, 769
AjnI CCWGG 1 cut(s) 203
AluBI AGCT 5 cut(s) 148, 168, 486, 613, 772
AluI AGCT 5 cut(s) 148, 168, 486, 613, 772
Alw21I GWGCWC 1 cut(s) 150
Alw26I GTCTC 3 cut(s) 113, 714, 757
AlwI GGATC 1 cut(s) 88
Aor13HI TCCGGA 1 cut(s) 716
AoxI GGCC 2 cut(s) 86, 459
ApeKI GCWGC 1 cut(s) 373
ApoI RAATTY 2 cut(s) 15, 712
Asp718I GGTACC 1 cut(s) 743
AspLEI GCGC 2 cut(s) 164, 546
AspS9I GGNCC 2 cut(s) 60, 624
AsuC2I CCSGG 1 cut(s) 218
AsuHPI GGTGA 1 cut(s) 344
AvaII GGWCC 2 cut(s) 60, 624
BaeI ACNNNNGTAYC 2 cut(s) 735, 768
BanI GGYRCC 2 cut(s) 466, 743
BanII GRGCYC 1 cut(s) 150
Bbv12I GWGCWC 1 cut(s) 150
BbvI GCAGC 1 cut(s) 360
BccI CCATC 2 cut(s) 329, 676
BceAI ACGGC 2 cut(s) 73, 427
BciT130I CCWGG 1 cut(s) 205
BcnI CCSGG 1 cut(s) 218
BcoDI GTCTC 3 cut(s) 113, 714, 757
BfuAI ACCTGC 1 cut(s) 478
BisI GCNGC 3 cut(s) 83, 86, 374
BlsI GCNGC 3 cut(s) 84, 87, 375
Bme1390I CCNGG 2 cut(s) 205, 218
Bme18I GGWCC 2 cut(s) 60, 624
BmgT120I GGNCC 2 cut(s) 60, 624
BmiI GGNNCC 3 cut(s) 61, 468, 745
BmrFI CCNGG 2 cut(s) 205, 218
BmrI ACTGGG 2 cut(s) 118, 749
BmuI ACTGGG 2 cut(s) 118, 749
BpmI CTGGAG 1 cut(s) 190
BpuEI CTTGAG 1 cut(s) 529
BpuMI CCSGG 1 cut(s) 218
BsaI GGTCTC 2 cut(s) 113, 714
BsaJI CCNNGG 5 cut(s) 83, 203, 204, 403, 462
BsaWI WCCGGW 1 cut(s) 716
Bsc4I CCNNNNNNNGG 1 cut(s) 130
Bse1I ACTGG 4 cut(s) 124, 651, 724, 744
BseAI TCCGGA 1 cut(s) 716
BseBI CCWGG 1 cut(s) 205
BseDI CCNNGG 5 cut(s) 83, 203, 204, 403, 462
BseGI GGATG 2 cut(s) 148, 349
BseLI CCNNNNNNNGG 1 cut(s) 130
BseMII CTCAG 1 cut(s) 762
BseNI ACTGG 4 cut(s) 124, 651, 724, 744
BseRI GAGGAG 2 cut(s) 236, 688
BseX3I CGGCCG 1 cut(s) 86
BseXI GCAGC 1 cut(s) 360
BseYI CCCAGC 1 cut(s) 299
Bsh1236I CGCG 3 cut(s) 47, 85, 639
Bsh1285I CGRYCG 1 cut(s) 89
BshFI GGCC 2 cut(s) 88, 461
BshNI GGYRCC 2 cut(s) 466, 743
BsiEI CGRYCG 1 cut(s) 89
BsiHKAI GWGCWC 1 cut(s) 150
BsiSI CCGG 2 cut(s) 218, 717
BslFI GGGAC 1 cut(s) 73
BslI CCNNNNNNNGG 1 cut(s) 130
BsmAI GTCTC 3 cut(s) 113, 714, 757
BsmFI GGGAC 1 cut(s) 73
BsnI GGCC 2 cut(s) 88, 461
Bso31I GGTCTC 2 cut(s) 113, 714
Bsp1286I GDGCHC 1 cut(s) 150
Bsp13I TCCGGA 1 cut(s) 716
Bsp143I GATC 2 cut(s) 93, 274
Bsp19I CCATGG 2 cut(s) 403, 462
BspACI CCGC 6 cut(s) 83, 85, 230, 627, 637, 760
BspANI GGCC 2 cut(s) 88, 461
BspCNI CTCAG 1 cut(s) 761
BspEI TCCGGA 1 cut(s) 716
BspFNI CGCG 3 cut(s) 47, 85, 639
BspLI GGNNCC 3 cut(s) 61, 468, 745
BspMI ACCTGC 1 cut(s) 478
BspPI GGATC 1 cut(s) 88
BspT107I GGYRCC 2 cut(s) 466, 743
BspTNI GGTCTC 2 cut(s) 113, 714
BsrBI CCGCTC 1 cut(s) 762
BsrI ACTGG 4 cut(s) 124, 651, 724, 744
BssECI CCNNGG 5 cut(s) 83, 203, 204, 403, 462
BssMI GATC 2 cut(s) 93, 274
BssT1I CCWWGG 2 cut(s) 403, 462
Bst2UI CCWGG 1 cut(s) 205
Bst4CI ACNGT 1 cut(s) 571
Bst6I CTCTTC 2 cut(s) 495, 665
BstC8I GCNNGC 1 cut(s) 166
BstDEI CTNAG 2 cut(s) 291, 748
BstDSI CCRYGG 3 cut(s) 83, 403, 462
BstF5I GGATG 2 cut(s) 148, 349
BstFNI CGCG 3 cut(s) 47, 85, 639
BstHHI GCGC 2 cut(s) 164, 546
BstKTI GATC 2 cut(s) 96, 277
BstMAI GTCTC 3 cut(s) 113, 714, 757
BstMBI GATC 2 cut(s) 93, 274
BstMCI CGRYCG 1 cut(s) 89
BstMWI GCNNNNNNNGC 1 cut(s) 541
BstNI CCWGG 1 cut(s) 205
BstSCI CCNGG 2 cut(s) 203, 216
BstUI CGCG 3 cut(s) 47, 85, 639
BstV1I GCAGC 1 cut(s) 360
BstZI CGGCCG 1 cut(s) 86
BsuRI GGCC 2 cut(s) 88, 461
BtgI CCRYGG 3 cut(s) 83, 403, 462
BtsCI GGATG 2 cut(s) 148, 349
BtsI GCAGTG 1 cut(s) 157
BtsIMutI CAGTG 2 cut(s) 157, 731
BveI ACCTGC 1 cut(s) 478
Cac8I GCNNGC 1 cut(s) 166
CfoI GCGC 2 cut(s) 164, 546
Cfr13I GGNCC 2 cut(s) 60, 624
Cfr42I CCGCGG 1 cut(s) 86
Csp6I GTAC 1 cut(s) 744
CviAII CATG 3 cut(s) 404, 463, 798
CviQI GTAC 1 cut(s) 744
DdeI CTNAG 2 cut(s) 291, 748
DpnI GATC 2 cut(s) 95, 276
DpnII GATC 2 cut(s) 93, 274
EaeI YGGCCR 1 cut(s) 86
EagI CGGCCG 1 cut(s) 86
Eam1104I CTCTTC 2 cut(s) 495, 665
EarI CTCTTC 2 cut(s) 495, 665
Ecl136II GAGCTC 1 cut(s) 148
EclXI CGGCCG 1 cut(s) 86
Eco130I CCWWGG 2 cut(s) 403, 462
Eco24I GRGCYC 1 cut(s) 150
Eco31I GGTCTC 2 cut(s) 113, 714
Eco47I GGWCC 2 cut(s) 60, 624
Eco52I CGGCCG 1 cut(s) 86
Eco53kI GAGCTC 1 cut(s) 148
EcoICRI GAGCTC 1 cut(s) 148
EcoO109I RGGNCCY 1 cut(s) 60
EcoRI GAATTC 1 cut(s) 712
EcoRII CCWGG 1 cut(s) 203
EcoT14I CCWWGG 2 cut(s) 403, 462
EcoT38I GRGCYC 1 cut(s) 150
ErhI CCWWGG 2 cut(s) 403, 462
FaeI CATG 3 cut(s) 407, 466, 801
FalI AAGNNNNNCTT 4 cut(s) 470, 502, 515, 547
FaqI GGGAC 1 cut(s) 73
FatI CATG 3 cut(s) 403, 462, 797
Fnu4HI GCNGC 3 cut(s) 83, 86, 374
FokI GGATG 2 cut(s) 155, 356
FriOI GRGCYC 1 cut(s) 150
Fsp4HI GCNGC 3 cut(s) 83, 86, 374
GlaI GCGC 2 cut(s) 163, 545
GluI GCNGC 3 cut(s) 83, 86, 374
GsaI CCCAGC 1 cut(s) 303
GsuI CTGGAG 1 cut(s) 190
HaeIII GGCC 2 cut(s) 88, 461
HapII CCGG 2 cut(s) 218, 717
HhaI GCGC 2 cut(s) 164, 546
Hin1II CATG 3 cut(s) 407, 466, 801
Hin6I GCGC 2 cut(s) 162, 544
HinP1I GCGC 2 cut(s) 162, 544
HindIII AAGCTT 2 cut(s) 611, 770
HinfI GANTC 3 cut(s) 68, 577, 778
HpaII CCGG 2 cut(s) 218, 717
HphI GGTGA 1 cut(s) 344
Hpy166II GTNNAC 1 cut(s) 307
Hpy188I TCNGA 4 cut(s) 152, 582, 667, 777
Hpy188III TCNNGA 4 cut(s) 278, 508, 599, 717
Hpy8I GTNNAC 1 cut(s) 307
Hpy99I CGWCG 2 cut(s) 47, 655
HpyAV CCTTC 5 cut(s) 32, 73, 451, 545, 556
HpyCH4III ACNGT 1 cut(s) 571
HpyCH4V TGCA 1 cut(s) 590
HpyF10VI GCNNNNNNNGC 1 cut(s) 541
HpyF3I CTNAG 2 cut(s) 291, 748
Hsp92II CATG 3 cut(s) 407, 466, 801
HspAI GCGC 2 cut(s) 162, 544
Kpn2I TCCGGA 1 cut(s) 716
KpnI GGTACC 1 cut(s) 747
KspI CCGCGG 1 cut(s) 86
Kzo9I GATC 2 cut(s) 93, 274
Lsp1109I GCAGC 1 cut(s) 360
MaeIII GTNAC 2 cut(s) 407, 565
MalI GATC 2 cut(s) 95, 276
MbiI CCGCTC 1 cut(s) 762
MboI GATC 2 cut(s) 93, 274
MboII GAAGA 3 cut(s) 377, 512, 682
MhlI GDGCHC 1 cut(s) 150
MluCI AATT 4 cut(s) 15, 134, 323, 712
MlyI GAGTC 1 cut(s) 62
MroI TCCGGA 1 cut(s) 716
MseI TTAA 1 cut(s) 522
MspA1I CMGCKG 3 cut(s) 85, 168, 629
MspI CCGG 2 cut(s) 218, 717
MspR9I CCNGG 2 cut(s) 205, 218
MvaI CCWGG 1 cut(s) 205
MvnI CGCG 3 cut(s) 47, 85, 639
MwoI GCNNNNNNNGC 1 cut(s) 541
NciI CCSGG 1 cut(s) 218
NcoI CCATGG 2 cut(s) 403, 462
NdeII GATC 2 cut(s) 93, 274
NlaIII CATG 3 cut(s) 407, 466, 801
NlaIV GGNNCC 3 cut(s) 61, 468, 745
PaqCI CACCTGC 1 cut(s) 478
PasI CCCWGGG 1 cut(s) 204
PcsI WCGNNNNNNNCGW 1 cut(s) 63
PfeI GAWTC 2 cut(s) 577, 778
PfoI TCCNGGA 1 cut(s) 216
PkrI GCNGC 3 cut(s) 84, 87, 375
PleI GAGTC 1 cut(s) 62
PpsI GAGTC 1 cut(s) 62
PpuMI RGGWCCY 1 cut(s) 60
Psp124BI GAGCTC 1 cut(s) 150
Psp5II RGGWCCY 1 cut(s) 60
Psp6I CCWGG 1 cut(s) 203
PspFI CCCAGC 1 cut(s) 299
PspGI CCWGG 1 cut(s) 203
PspN4I GGNNCC 3 cut(s) 61, 468, 745
PspPI GGNCC 2 cut(s) 60, 624
PspPPI RGGWCCY 1 cut(s) 60
PvuII CAGCTG 1 cut(s) 168
RsaI GTAC 1 cut(s) 745
RsaNI GTAC 1 cut(s) 744
SacI GAGCTC 1 cut(s) 150
SacII CCGCGG 1 cut(s) 86
SaqAI TTAA 1 cut(s) 522
SatI GCNGC 3 cut(s) 83, 86, 374
Sau3AI GATC 2 cut(s) 93, 274
Sau96I GGNCC 2 cut(s) 60, 624
SchI GAGTC 1 cut(s) 62
ScrFI CCNGG 2 cut(s) 205, 218
SduI GDGCHC 1 cut(s) 150
Sfr303I CCGCGG 1 cut(s) 86
SgrBI CCGCGG 1 cut(s) 86
SinI GGWCC 2 cut(s) 60, 624
SmlI CTYRAG 1 cut(s) 508
SmoI CTYRAG 1 cut(s) 508
Sse9I AATT 4 cut(s) 15, 134, 323, 712
SsiI CCGC 6 cut(s) 83, 85, 230, 627, 637, 760
SstI GAGCTC 1 cut(s) 150
StyD4I CCNGG 2 cut(s) 203, 216
StyI CCWWGG 2 cut(s) 403, 462
TaaI ACNGT 1 cut(s) 571
TaqI TCGA 4 cut(s) 66, 273, 279, 598
TasI AATT 4 cut(s) 15, 134, 323, 712
TauI GCSGC 2 cut(s) 85, 88
TfiI GAWTC 2 cut(s) 577, 778
Tru1I TTAA 1 cut(s) 522
Tru9I TTAA 1 cut(s) 522
TscAI CASTG 2 cut(s) 164, 731
TseI GCWGC 1 cut(s) 373
TspDTI ATGAA 2 cut(s) 17, 513
TspGWI ACGGA 1 cut(s) 31
TspRI CASTG 2 cut(s) 164, 731
VpaK11BI GGWCC 2 cut(s) 60, 624
XapI RAATTY 2 cut(s) 15, 712
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.