AT4G25770

Putative serine esterase (DUF676)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0007465
Physical Location & Seq
Reverse (-)
13117505 .. 13120368
2864 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G25770.1

Sequence Viewer

Length: 1257 bp
ATGATCGCGTCCTTCTCCAGATTCCAAATCTCAATCCCCGATTTGATTCCGTCTTCACCGCGATTTCTTCCGCCTAGTCAAAACCAGTTCCGTTTCAGATTATACCTTCAATTTCCAGCTCAACCTAAGAATCTCTGTCAAATGGAGTGGATGAATCGTCTTGGAATCGGTTGCTTTGCGGCGAATCGAAGCAAAAGAGAGCTGACGACGGAGATCGACAACGGCGGAGAAGACTTTTTCGATGCGGATGTTATGGAGTCTGCGGAGAAGCCTGATCATTTGGTGGTCATGGTTAACGGAATCGTTGGCAGTGCGGCGGATTGGAAATATGCGGCGGAGCAGTTCGTGAAGAAGTTCCCGGATAAAGTACTAGTGCACCGTAGCGAGAGTAATAGTGCAACGTTGACATTTGATGGTGTTGATAAAATGGGTGAAAGGCTAGCTAATGAGGTTTTGGGTGTTGTTAAGCATAGAAGTGGGCTGAAGAAGATATCTTTTGTGGCTCATTCATTAGGAGGTCTTGTTGCAAGATATGCCATAGGGAAGCTCTATGAACAGCCAGGGGAAGTCGACTCTCTGGACTCTCCTTCGAAGGAGAAGAGCGCAAGAGGAGGCGAAATTGCTGGGTTAGAACCTATGAACTTTATAACTTTCGCAACGCCTCATCTTGGTTCAAGAGGACATAGACAGTTCCCGATTCTTTGTGGCCTTCCGTTTCTTGAAAGAACAGCATCCCAAACTGCACATTTGGCTGCTGGGAGGACCGGAAAGCATTTGTTTCTGGTGGACAACGATGATGGAAATGCTCCACTTCTTATCCGGATGGCTACAGATTCTGATGACTTGAAATTCATATCAGCTTTAAATGCTTTCAAGCGTCGTGTGGCATATGCAAACGTGAATTTTGATTCTATGGTTGGATGGAGGACATCTTCAATTCGCCGTCCAAATGAACTCCCAAAGCCAAATCTTCTAGCAACGGATCCAAACTATCCACACATTGTATACGTAGAACGTGGAAATGTGGACAATGGTAGTTGCCAGTCAACTTCTACAGTAGTAACAGAGCAAGATACTGATCTTGAAGAGGAAATGATACATGGACTCAGTCAACTATCTTGGGAAAGAGTAGATGTCAGCTTTCACAATAGCAAGCAACGATACGTCGCGCATAGTACCATCCAGGTGAAAACATATTGGTTACATTCTGATGGCAAAGATGTTGTCTTCCACATGATGGATCATTTCGGTCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF676

Protein Analysis

418

Amino Acids

46.76

Weight (kDa)

6.44

Isoelectric Point (pI)

36.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF676 PF05057 89 - 310 5e-75 Putative serine esterase (DUF676)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 647
AccB7I CCANNNNNTGG 1 cut(s) 1237
AccI GTMKAC 2 cut(s) 570, 1005
AccII CGCG 3 cut(s) 8, 61, 1169
AccIII TCCGGA 1 cut(s) 819
AclI AACGTT 1 cut(s) 401
AclWI GGATC 3 cut(s) 977, 990, 1248
AcsI RAATTY 2 cut(s) 848, 901
AcuI CTGAAG 1 cut(s) 503
AfaI GTAC 2 cut(s) 369, 1177
AfiI CCNNNNNNNGG 2 cut(s) 668, 1237
AgsI TTSAA 7 cut(s) 110, 675, 722, 847, 874, 936, 1085
AhlI ACTAGT 1 cut(s) 370
AjnI CCWGG 2 cut(s) 559, 1182
AluBI AGCT 6 cut(s) 119, 202, 443, 547, 860, 1140
AluI AGCT 6 cut(s) 119, 202, 443, 547, 860, 1140
Alw21I GWGCWC 1 cut(s) 378
Alw44I GTGCAC 1 cut(s) 374
AlwI GGATC 3 cut(s) 977, 990, 1248
AlwNI CAGNNNCTG 1 cut(s) 836
Aor13HI TCCGGA 1 cut(s) 819
AoxI GGCC 1 cut(s) 706
ApaLI GTGCAC 1 cut(s) 374
ApeKI GCWGC 1 cut(s) 752
ApoI RAATTY 2 cut(s) 848, 901
ArsI GACNNNNNNTTYG 2 cut(s) 1209, 1241
Asp700I GAANNNNTTC 1 cut(s) 353
AspLEI GCGC 2 cut(s) 605, 1171
AspS9I GGNCC 1 cut(s) 762
AsuC2I CCSGG 1 cut(s) 359
AsuHPI GGTGA 3 cut(s) 48, 443, 1198
AsuII TTCGAA 1 cut(s) 590
AsuNHI GCTAGC 1 cut(s) 439
AvaII GGWCC 1 cut(s) 762
BaeGI GKGCMC 1 cut(s) 378
BamHI GGATCC 1 cut(s) 982
BbsI GAAGAC 3 cut(s) 45, 237, 1219
Bbv12I GWGCWC 1 cut(s) 378
BbvI GCAGC 1 cut(s) 739
BccI CCATC 7 cut(s) 407, 791, 817, 915, 1187, 1205, 1231
BceAI ACGGC 2 cut(s) 238, 927
BciT130I CCWGG 2 cut(s) 561, 1184
BclI TGATCA 1 cut(s) 274
BcnI CCSGG 1 cut(s) 359
BcuI ACTAGT 1 cut(s) 370
BfaI CTAG 5 cut(s) 75, 371, 440, 974, 1255
BfmI CTRYAG 2 cut(s) 828, 1053
BisI GCNGC 4 cut(s) 180, 315, 333, 753
BlsI GCNGC 4 cut(s) 181, 316, 334, 754
BmcAI AGTACT 1 cut(s) 369
Bme1390I CCNGG 3 cut(s) 359, 561, 1184
Bme18I GGWCC 1 cut(s) 762
BmgT120I GGNCC 1 cut(s) 762
BmiI GGNNCC 1 cut(s) 984
BmrFI CCNGG 3 cut(s) 359, 561, 1184
BmsI GCATC 2 cut(s) 232, 740
BmtI GCTAGC 1 cut(s) 443
BpiI GAAGAC 3 cut(s) 45, 237, 1219
Bpu14I TTCGAA 1 cut(s) 590
BpuMI CCSGG 1 cut(s) 359
BsaAI YACGTR 1 cut(s) 1009
BsaBI GATNNNNATC 1 cut(s) 1077
BsaJI CCNNGG 1 cut(s) 560
BsaWI WCCGGW 2 cut(s) 764, 819
BsaXI ACNNNNNCTCC 2 cut(s) 507, 537
Bsc4I CCNNNNNNNGG 2 cut(s) 668, 1237
Bse1I ACTGG 2 cut(s) 85, 1042
Bse8I GATNNNNATC 1 cut(s) 1077
BseAI TCCGGA 1 cut(s) 819
BseBI CCWGG 2 cut(s) 561, 1184
BseDI CCNNGG 1 cut(s) 560
BseGI GGATG 6 cut(s) 156, 253, 731, 828, 926, 1179
BseJI GATNNNNATC 1 cut(s) 1077
BseLI CCNNNNNNNGG 2 cut(s) 668, 1237
BseMII CTCAG 1 cut(s) 1120
BseNI ACTGG 2 cut(s) 85, 1042
BseRI GAGGAG 1 cut(s) 624
BseSI GKGCMC 1 cut(s) 378
BseXI GCAGC 1 cut(s) 739
BseYI CCCAGC 2 cut(s) 623, 755
BsgI GTGCAG 1 cut(s) 726
Bsh1236I CGCG 3 cut(s) 8, 61, 1169
BshFI GGCC 1 cut(s) 708
BsiHKAI GWGCWC 1 cut(s) 378
BsiSI CCGG 3 cut(s) 359, 765, 820
BslI CCNNNNNNNGG 2 cut(s) 668, 1237
BsnI GGCC 1 cut(s) 708
Bsp119I TTCGAA 1 cut(s) 590
Bsp1286I GDGCHC 1 cut(s) 378
Bsp13I TCCGGA 1 cut(s) 819
Bsp143I GATC 6 cut(s) 3, 213, 274, 982, 1078, 1240
BspANI GGCC 1 cut(s) 708
BspCNI CTCAG 1 cut(s) 1119
BspEI TCCGGA 1 cut(s) 819
BspFNI CGCG 3 cut(s) 8, 61, 1169
BspLI GGNNCC 1 cut(s) 984
BspOI GCTAGC 1 cut(s) 443
BspPI GGATC 3 cut(s) 977, 990, 1248
BspQI GCTCTTC 1 cut(s) 593
BspT104I TTCGAA 1 cut(s) 590
BsrI ACTGG 2 cut(s) 85, 1042
BssECI CCNNGG 1 cut(s) 560
BssMI GATC 6 cut(s) 3, 213, 274, 982, 1078, 1240
BssNAI GTATAC 1 cut(s) 1006
Bst1107I GTATAC 1 cut(s) 1006
Bst2UI CCWGG 2 cut(s) 561, 1184
Bst4CI ACNGT 3 cut(s) 380, 690, 1057
Bst6I CTCTTC 2 cut(s) 593, 1080
BstAPI GCANNNNNTGC 1 cut(s) 533
BstBAI YACGTR 1 cut(s) 1009
BstBI TTCGAA 1 cut(s) 590
BstC8I GCNNGC 2 cut(s) 441, 1154
BstDEI CTNAG 2 cut(s) 126, 1106
BstF5I GGATG 6 cut(s) 156, 253, 731, 828, 926, 1179
BstFNI CGCG 3 cut(s) 8, 61, 1169
BstHHI GCGC 2 cut(s) 605, 1171
BstKTI GATC 6 cut(s) 6, 216, 277, 985, 1081, 1243
BstMBI GATC 6 cut(s) 3, 213, 274, 982, 1078, 1240
BstMWI GCNNNNNNNGC 3 cut(s) 533, 749, 866
BstNI CCWGG 2 cut(s) 561, 1184
BstSCI CCNGG 3 cut(s) 357, 559, 1182
BstSFI CTRYAG 2 cut(s) 828, 1053
BstSLI GKGCMC 1 cut(s) 378
BstSNI TACGTA 1 cut(s) 1009
BstUI CGCG 3 cut(s) 8, 61, 1169
BstV1I GCAGC 1 cut(s) 739
BstV2I GAAGAC 3 cut(s) 45, 237, 1219
BstX2I RGATCY 1 cut(s) 982
BstYI RGATCY 1 cut(s) 982
BstZ17I GTATAC 1 cut(s) 1006
BsuRI GGCC 1 cut(s) 708
BtsCI GGATG 6 cut(s) 156, 253, 731, 828, 926, 1179
BtsI GCAGTG 1 cut(s) 316
BtsIMutI CAGTG 1 cut(s) 316
Cac8I GCNNGC 2 cut(s) 441, 1154
CaiI CAGNNNCTG 1 cut(s) 836
CfoI GCGC 2 cut(s) 605, 1171
Cfr13I GGNCC 1 cut(s) 762
CseI GACGC 1 cut(s) 866
Csp6I GTAC 2 cut(s) 368, 1176
CspCI CAANNNNNGTGG 2 cut(s) 128, 163
CviAII CATG 3 cut(s) 289, 1100, 1234
CviQI GTAC 2 cut(s) 368, 1176
DdeI CTNAG 2 cut(s) 126, 1106
DpnI GATC 6 cut(s) 5, 215, 276, 984, 1080, 1242
DpnII GATC 6 cut(s) 3, 213, 274, 982, 1078, 1240
DraI TTTAAA 1 cut(s) 864
Eam1104I CTCTTC 2 cut(s) 593, 1080
EarI CTCTTC 2 cut(s) 593, 1080
EciI GGCGGA 4 cut(s) 60, 240, 332, 350
Eco105I TACGTA 1 cut(s) 1009
Eco32I GATATC 1 cut(s) 492
Eco47I GGWCC 1 cut(s) 762
Eco57I CTGAAG 1 cut(s) 503
EcoRII CCWGG 2 cut(s) 559, 1182
EcoRV GATATC 1 cut(s) 492
FaeI CATG 3 cut(s) 292, 1103, 1237
FatI CATG 3 cut(s) 288, 1099, 1233
FauNDI CATATG 1 cut(s) 889
FbaI TGATCA 1 cut(s) 274
FblI GTMKAC 2 cut(s) 570, 1005
Fnu4HI GCNGC 4 cut(s) 180, 315, 333, 753
FokI GGATG 6 cut(s) 163, 260, 718, 835, 933, 1166
Fsp4HI GCNGC 4 cut(s) 180, 315, 333, 753
FspBI CTAG 5 cut(s) 75, 371, 440, 974, 1255
GlaI GCGC 2 cut(s) 604, 1170
GluI GCNGC 4 cut(s) 180, 315, 333, 753
GsaI CCCAGC 2 cut(s) 627, 759
HaeIII GGCC 1 cut(s) 708
HapII CCGG 3 cut(s) 359, 765, 820
HgaI GACGC 1 cut(s) 866
HhaI GCGC 2 cut(s) 605, 1171
Hin1II CATG 3 cut(s) 292, 1103, 1237
Hin6I GCGC 2 cut(s) 603, 1169
HinP1I GCGC 2 cut(s) 603, 1169
HincII GTYRAC 5 cut(s) 295, 405, 571, 1047, 1112
HindII GTYRAC 5 cut(s) 295, 405, 571, 1047, 1112
HpaI GTTAAC 1 cut(s) 295
HpaII CCGG 3 cut(s) 359, 765, 820
HphI GGTGA 3 cut(s) 48, 443, 1198
Hpy166II GTNNAC 9 cut(s) 295, 376, 405, 571, 787, 1006, 1027, 1047, 1112
Hpy188I TCNGA 3 cut(s) 98, 838, 1210
Hpy188III TCNNGA 8 cut(s) 18, 346, 578, 675, 694, 719, 820, 1082
Hpy8I GTNNAC 9 cut(s) 295, 376, 405, 571, 787, 1006, 1027, 1047, 1112
Hpy99I CGWCG 3 cut(s) 211, 882, 1169
HpyAV CCTTC 5 cut(s) 22, 116, 586, 597, 719
HpyCH4III ACNGT 3 cut(s) 380, 690, 1057
HpyCH4IV ACGT 5 cut(s) 401, 897, 1008, 1015, 1164
HpyCH4V TGCA 5 cut(s) 376, 398, 527, 743, 893
HpyF10VI GCNNNNNNNGC 3 cut(s) 533, 749, 866
HpyF3I CTNAG 2 cut(s) 126, 1106
HpySE526I ACGT 5 cut(s) 401, 897, 1008, 1015, 1164
Hsp92II CATG 3 cut(s) 292, 1103, 1237
HspAI GCGC 2 cut(s) 603, 1169
Kpn2I TCCGGA 1 cut(s) 819
Ksp22I TGATCA 1 cut(s) 274
KspAI GTTAAC 1 cut(s) 295
Kzo9I GATC 6 cut(s) 3, 213, 274, 982, 1078, 1240
LguI GCTCTTC 1 cut(s) 593
LmnI GCTCC 2 cut(s) 337, 811
Lsp1109I GCAGC 1 cut(s) 739
LweI GCATC 2 cut(s) 232, 740
MaeI CTAG 5 cut(s) 75, 371, 440, 974, 1255
MaeII ACGT 5 cut(s) 401, 897, 1008, 1015, 1164
MaeIII GTNAC 2 cut(s) 1060, 1200
MalI GATC 6 cut(s) 5, 215, 276, 984, 1080, 1242
MboI GATC 6 cut(s) 3, 213, 274, 982, 1078, 1240
MflI RGATCY 1 cut(s) 982
MhlI GDGCHC 1 cut(s) 378
MluCI AATT 5 cut(s) 110, 618, 848, 901, 936
MlyI GAGTC 4 cut(s) 266, 566, 575, 1098
MmeI TCCRAC 1 cut(s) 898
MnlI CCTC 9 cut(s) 442, 509, 602, 605, 671, 672, 753, 918, 1081
MroI TCCGGA 1 cut(s) 819
MroXI GAANNNNTTC 1 cut(s) 353
MseI TTAA 3 cut(s) 294, 465, 863
MslI CAYNNNNRTG 3 cut(s) 474, 1184, 1209
MspI CCGG 3 cut(s) 359, 765, 820
MspR9I CCNGG 3 cut(s) 359, 561, 1184
MvaI CCWGG 2 cut(s) 561, 1184
MvnI CGCG 3 cut(s) 8, 61, 1169
MwoI GCNNNNNNNGC 3 cut(s) 533, 749, 866
NciI CCSGG 1 cut(s) 359
NdeI CATATG 1 cut(s) 889
NdeII GATC 6 cut(s) 3, 213, 274, 982, 1078, 1240
NheI GCTAGC 1 cut(s) 439
NlaIII CATG 3 cut(s) 292, 1103, 1237
NlaIV GGNNCC 1 cut(s) 984
NspV TTCGAA 1 cut(s) 590
PciSI GCTCTTC 1 cut(s) 593
PdmI GAANNNNTTC 1 cut(s) 353
PflFI GACNNNGTC 1 cut(s) 1107
PflMI CCANNNNNTGG 1 cut(s) 1237
PfoI TCCNGGA 1 cut(s) 357
PkrI GCNGC 4 cut(s) 181, 316, 334, 754
PleI GAGTC 4 cut(s) 265, 566, 575, 1098
PpsI GAGTC 4 cut(s) 265, 566, 575, 1098
Ppu21I YACGTR 1 cut(s) 1009
PsiI TTATAA 1 cut(s) 647
Psp1406I AACGTT 1 cut(s) 401
Psp6I CCWGG 2 cut(s) 559, 1182
PspFI CCCAGC 2 cut(s) 623, 755
PspGI CCWGG 2 cut(s) 559, 1182
PspN4I GGNNCC 1 cut(s) 984
PspPI GGNCC 1 cut(s) 762
PstNI CAGNNNCTG 1 cut(s) 836
PsuI RGATCY 1 cut(s) 982
PsyI GACNNNGTC 1 cut(s) 1107
RsaI GTAC 2 cut(s) 369, 1177
RsaNI GTAC 2 cut(s) 368, 1176
RseI CAYNNNNRTG 3 cut(s) 474, 1184, 1209
SalI GTCGAC 1 cut(s) 569
SapI GCTCTTC 1 cut(s) 593
SaqAI TTAA 3 cut(s) 294, 465, 863
SatI GCNGC 4 cut(s) 180, 315, 333, 753
Sau3AI GATC 6 cut(s) 3, 213, 274, 982, 1078, 1240
Sau96I GGNCC 1 cut(s) 762
ScaI AGTACT 1 cut(s) 369
SchI GAGTC 4 cut(s) 266, 566, 575, 1098
ScrFI CCNGG 3 cut(s) 359, 561, 1184
SduI GDGCHC 1 cut(s) 378
SfaNI GCATC 2 cut(s) 232, 740
SfcI CTRYAG 2 cut(s) 828, 1053
SfuI TTCGAA 1 cut(s) 590
SinI GGWCC 1 cut(s) 762
SmiMI CAYNNNNRTG 3 cut(s) 474, 1184, 1209
SnaBI TACGTA 1 cut(s) 1009
SpeI ACTAGT 1 cut(s) 370
Sse9I AATT 5 cut(s) 110, 618, 848, 901, 936
SspMI CTAG 5 cut(s) 75, 371, 440, 974, 1255
StyD4I CCNGG 3 cut(s) 357, 559, 1182
TaaI ACNGT 3 cut(s) 380, 690, 1057
TaiI ACGT 5 cut(s) 404, 900, 1011, 1018, 1167
TaqI TCGA 5 cut(s) 187, 216, 240, 570, 590
TaqII GACCGA 1 cut(s) 1238
TasI AATT 5 cut(s) 110, 618, 848, 901, 936
TatI WGTACW 1 cut(s) 367
TauI GCSGC 3 cut(s) 182, 317, 335
Tru1I TTAA 3 cut(s) 294, 465, 863
Tru9I TTAA 3 cut(s) 294, 465, 863
TscAI CASTG 1 cut(s) 316
TseI GCWGC 1 cut(s) 752
TspDTI ATGAA 6 cut(s) 167, 498, 567, 653, 841, 966
TspGWI ACGGA 6 cut(s) 39, 80, 224, 312, 702, 995
TspRI CASTG 1 cut(s) 316
Tth111I GACNNNGTC 1 cut(s) 1107
Van91I CCANNNNNTGG 1 cut(s) 1237
VneI GTGCAC 1 cut(s) 374
VpaK11BI GGWCC 1 cut(s) 762
XapI RAATTY 2 cut(s) 848, 901
XmiI GTMKAC 2 cut(s) 570, 1005
XmnI GAANNNNTTC 1 cut(s) 353
XspI CTAG 5 cut(s) 75, 371, 440, 974, 1255
ZrmI AGTACT 1 cut(s) 369
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.