Rroxscaffold_5G00348200

Putative serine esterase (DUF676)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0007465
Physical Location & Seq
Forward (+)
20191430 .. 20198308
6879 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00348200.1

Sequence Viewer

Length: 1194 bp
ATGATTGCAGCTACTTCTCCAGTCAACTCCGCATCCCCGATCCCCGCCCCCAACCCGACCCGAACCCGCCGCCCGCGCCAGCTCCGCCTAGGGTTTCCTTCCTTCTCCGAGCCAAGGCTCTGTGCGACGAGGCCCAGGATGGACTTCCTGCACAGAATCGTAGGCGGAGGCTGCTTCCGGGGATCCACGCGCCAGGGGCTGAAGGTCGAGATTGATAGCGGCGGCGAGGATATCTTTGCCGCCGCCGCCAACAACGCCGTTCCCGAACATCTCGTCGTCATGGTCAATGGAATTATCGGCAGTGCTTCAGATTGGAGATATGCTGCAGATCAGTTTGTGAAAAAGCTTCCTGATAAAGTAATTGTGCACCGTATTAGCTGTTGTCAGAAAAAGGTCAGAGGTGCGGAAAATTTCCTTTGTCGCTCACTCTTTGGGGGGTTGGTTGCAAGGTATGCTATCGGGAGGCTGTACGAACCTTTTCCAAAACCCGAACCCTCAGGTGATAATGGTAATTGCTTAATTGAAGGGAATACAAATAATTTGACACAATCCCTTGACCAGCCTCACCAAGGTACGATTGCTGGATTACAAGCTATGAATTTTATAACGGTTGCAACACCACATCTTGGCTCAAGGGGAAATAAACAGCTCCCATTTCTCTGTGGTCTTCCCTTTCTTGAAAGAAGAGCTTCTCAAGCTGCCCACTTTATTGTGGGGAGATCTGGGAAGCATCTCTTCCTAACAGATAAAGACGATGGCAAACCTCCCCTTCTTCTTCAAATGGTTAATGACTCTGACGACCTGAAATTCATGTCAGCATTACGTGCATTTAAGCGTCGTGTGGCATATGCTAATGCAAATTTTGATCGTATCCTTCACTGGTCCAATCTACTTGTAACAGATGAGAAATATCCACATATTGTACATGTGAAGCAAGAAACTTTTGATGATATTCATAATAATGCATCTTCAGCTGCTGAAAACCAAAATATCGACTTGGAAGAGGAGATGATCAGAGGTCTCACTCAAATACCATGGGAACGTGTAGATGTCAGCTTTCAAAAGAGCCGACAAAGATACATTGCTCATAATACCATTCAGGCAAGTTTGTTTAGGCTGTGGGATTCAGGACTGGAGGCCTGTGGTGTGGCGTCTGGCCTAAGAAACAATAGTCTGGCTCGACTTTTGGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF676

Protein Analysis

397

Amino Acids

44.09

Weight (kDa)

9.2

Isoelectric Point (pI)

44.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF676 PF05057 88 - 126 1.5e-07 Putative serine esterase (DUF676)
DUF676 PF05057 142 - 291 1.4e-33 Putative serine esterase (DUF676)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 605
AccB7I CCANNNNNTGG 1 cut(s) 626
AccII CGCG 2 cut(s) 76, 190
AclWI GGATC 3 cut(s) 34, 177, 190
AcsI RAATTY 4 cut(s) 409, 598, 806, 859
AcuI CTGAAG 3 cut(s) 221, 291, 954
AcyI GRCGYC 1 cut(s) 1151
AfaI GTAC 3 cut(s) 470, 574, 924
AfiI CCNNNNNNNGG 3 cut(s) 114, 569, 626
AflIII ACRYGT 2 cut(s) 925, 1042
AgsI TTSAA 4 cut(s) 524, 680, 779, 1061
AjnI CCWGG 2 cut(s) 134, 192
Alw21I GWGCWC 1 cut(s) 369
Alw26I GTCTC 1 cut(s) 1025
Alw44I GTGCAC 1 cut(s) 365
AlwI GGATC 3 cut(s) 34, 177, 190
AlwNI CAGNNNCTG 2 cut(s) 199, 977
AoxI GGCC 3 cut(s) 131, 1137, 1156
ApaLI GTGCAC 1 cut(s) 365
ApeKI GCWGC 5 cut(s) 8, 171, 323, 698, 974
ApoI RAATTY 4 cut(s) 409, 598, 806, 859
ArsI GACNNNNNNTTYG 2 cut(s) 258, 290
Asp700I GAANNNNTTC 2 cut(s) 477, 688
AspA2I CCTAGG 1 cut(s) 88
AspLEI GCGC 2 cut(s) 78, 192
AspS9I GGNCC 2 cut(s) 132, 882
AsuC2I CCSGG 1 cut(s) 179
AsuHPI GGTGA 2 cut(s) 512, 557
AvaII GGWCC 1 cut(s) 882
AvrII CCTAGG 1 cut(s) 88
AxyI CCTNAGG 1 cut(s) 496
BaeGI GKGCMC 1 cut(s) 369
BamHI GGATCC 1 cut(s) 182
BbsI GAAGAC 1 cut(s) 659
Bbv12I GWGCWC 1 cut(s) 369
BbvI GCAGC 5 cut(s) 20, 158, 310, 685, 961
BccI CCATC 2 cut(s) 133, 749
BceAI ACGGC 1 cut(s) 242
BciT130I CCWGG 2 cut(s) 136, 194
BciVI GTATCC 1 cut(s) 881
BclI TGATCA 1 cut(s) 1011
BcnI CCSGG 1 cut(s) 179
BcoDI GTCTC 1 cut(s) 1025
BfaI CTAG 1 cut(s) 89
BfmI CTRYAG 1 cut(s) 324
BfuI GTATCC 1 cut(s) 881
BglII AGATCT 1 cut(s) 719
BlnI CCTAGG 1 cut(s) 88
Bme1390I CCNGG 3 cut(s) 136, 179, 194
Bme18I GGWCC 1 cut(s) 882
BmgT120I GGNCC 2 cut(s) 132, 882
BmiI GGNNCC 1 cut(s) 184
BmrFI CCNGG 3 cut(s) 136, 179, 194
BmsI GCATC 3 cut(s) 41, 739, 974
BpiI GAAGAC 1 cut(s) 659
BpmI CTGGAG 1 cut(s) 1154
BpuEI CTTGAG 2 cut(s) 616, 678
BpuMI CCSGG 1 cut(s) 179
BsaAI YACGTR 1 cut(s) 824
BsaHI GRCGYC 1 cut(s) 1151
BsaI GGTCTC 1 cut(s) 1025
BsaJI CCNNGG 7 cut(s) 88, 113, 134, 178, 193, 568, 1034
Bsc4I CCNNNNNNNGG 3 cut(s) 114, 569, 626
Bse1I ACTGG 3 cut(s) 20, 884, 1137
Bse21I CCTNAGG 1 cut(s) 496
Bse3DI GCAATG 1 cut(s) 1080
BseBI CCWGG 2 cut(s) 136, 194
BseDI CCNNGG 7 cut(s) 88, 113, 134, 178, 193, 568, 1034
BseGI GGATG 2 cut(s) 32, 144
BseLI CCNNNNNNNGG 3 cut(s) 114, 569, 626
BseMI GCAATG 1 cut(s) 1080
BseMII CTCAG 1 cut(s) 510
BseNI ACTGG 3 cut(s) 20, 884, 1137
BseRI GAGGAG 1 cut(s) 1019
BseSI GKGCMC 1 cut(s) 369
BseXI GCAGC 5 cut(s) 20, 158, 310, 685, 961
BsgI GTGCAG 1 cut(s) 134
Bsh1236I CGCG 2 cut(s) 76, 190
BshFI GGCC 3 cut(s) 133, 1139, 1158
BsiHKAI GWGCWC 1 cut(s) 369
BsiSI CCGG 1 cut(s) 178
BslI CCNNNNNNNGG 3 cut(s) 114, 569, 626
BsmAI GTCTC 1 cut(s) 1025
BsnI GGCC 3 cut(s) 133, 1139, 1158
Bso31I GGTCTC 1 cut(s) 1025
Bsp1286I GDGCHC 1 cut(s) 369
Bsp1407I TGTACA 1 cut(s) 922
Bsp143I GATC 6 cut(s) 39, 182, 328, 719, 865, 1011
Bsp19I CCATGG 1 cut(s) 1034
BspANI GGCC 3 cut(s) 133, 1139, 1158
BspCNI CTCAG 1 cut(s) 509
BspFNI CGCG 2 cut(s) 76, 190
BspLI GGNNCC 1 cut(s) 184
BspMAI CTGCAG 1 cut(s) 328
BspPI GGATC 3 cut(s) 34, 177, 190
BspQI GCTCTTC 1 cut(s) 679
BspTNI GGTCTC 1 cut(s) 1025
BsrDI GCAATG 1 cut(s) 1080
BsrGI TGTACA 1 cut(s) 922
BsrI ACTGG 3 cut(s) 20, 884, 1137
BssECI CCNNGG 7 cut(s) 88, 113, 134, 178, 193, 568, 1034
BssMI GATC 6 cut(s) 39, 182, 328, 719, 865, 1011
BssNI GRCGYC 1 cut(s) 1151
BssT1I CCWWGG 4 cut(s) 88, 113, 568, 1034
Bst2UI CCWGG 2 cut(s) 136, 194
Bst4CI ACNGT 2 cut(s) 371, 610
Bst6I CTCTTC 3 cut(s) 679, 740, 996
BstACI GRCGYC 1 cut(s) 1151
BstAPI GCANNNNNTGC 2 cut(s) 452, 824
BstAUI TGTACA 1 cut(s) 922
BstBAI YACGTR 1 cut(s) 824
BstC8I GCNNGC 2 cut(s) 74, 80
BstDEI CTNAG 2 cut(s) 496, 1160
BstDSI CCRYGG 1 cut(s) 1034
BstENI CCTNNNNNAGG 1 cut(s) 567
BstF5I GGATG 2 cut(s) 32, 144
BstFNI CGCG 2 cut(s) 76, 190
BstHHI GCGC 2 cut(s) 78, 192
BstKTI GATC 6 cut(s) 42, 185, 331, 722, 868, 1014
BstMAI GTCTC 1 cut(s) 1025
BstMBI GATC 6 cut(s) 39, 182, 328, 719, 865, 1011
BstNI CCWGG 2 cut(s) 136, 194
BstNSI RCATGY 1 cut(s) 929
BstSCI CCNGG 3 cut(s) 134, 177, 192
BstSFI CTRYAG 1 cut(s) 324
BstSLI GKGCMC 1 cut(s) 369
BstUI CGCG 2 cut(s) 76, 190
BstV1I GCAGC 5 cut(s) 20, 158, 310, 685, 961
BstV2I GAAGAC 1 cut(s) 659
BstX2I RGATCY 2 cut(s) 182, 719
BstYI RGATCY 2 cut(s) 182, 719
Bsu36I CCTNAGG 1 cut(s) 496
BsuI GTATCC 1 cut(s) 881
BsuRI GGCC 3 cut(s) 133, 1139, 1158
BtgI CCRYGG 1 cut(s) 1034
BtsCI GGATG 2 cut(s) 32, 144
BtsI GCAGTG 1 cut(s) 307
BtsIMutI CAGTG 2 cut(s) 307, 877
Cac8I GCNNGC 2 cut(s) 74, 80
CaiI CAGNNNCTG 2 cut(s) 199, 977
CfoI GCGC 2 cut(s) 78, 192
Cfr13I GGNCC 2 cut(s) 132, 882
CseI GACGC 2 cut(s) 824, 1140
Csp6I GTAC 3 cut(s) 469, 573, 923
CviAII CATG 4 cut(s) 280, 811, 926, 1035
CviQI GTAC 3 cut(s) 469, 573, 923
DdeI CTNAG 2 cut(s) 496, 1160
DpnI GATC 6 cut(s) 41, 184, 330, 721, 867, 1013
DpnII GATC 6 cut(s) 39, 182, 328, 719, 865, 1011
Eam1104I CTCTTC 3 cut(s) 679, 740, 996
EarI CTCTTC 3 cut(s) 679, 740, 996
EciI GGCGGA 2 cut(s) 74, 180
Eco130I CCWWGG 4 cut(s) 88, 113, 568, 1034
Eco147I AGGCCT 1 cut(s) 1139
Eco31I GGTCTC 1 cut(s) 1025
Eco32I GATATC 1 cut(s) 232
Eco47I GGWCC 1 cut(s) 882
Eco57I CTGAAG 3 cut(s) 221, 291, 954
Eco81I CCTNAGG 1 cut(s) 496
EcoNI CCTNNNNNAGG 1 cut(s) 567
EcoRII CCWGG 2 cut(s) 134, 192
EcoRV GATATC 1 cut(s) 232
EcoT14I CCWWGG 4 cut(s) 88, 113, 568, 1034
EcoT22I ATGCAT 1 cut(s) 967
ErhI CCWWGG 4 cut(s) 88, 113, 568, 1034
FaeI CATG 4 cut(s) 283, 814, 929, 1038
FalI AAGNNNNNCTT 4 cut(s) 673, 705, 719, 751
FatI CATG 4 cut(s) 279, 810, 925, 1034
FauI CCCGC 3 cut(s) 52, 74, 81
FauNDI CATATG 1 cut(s) 847
FbaI TGATCA 1 cut(s) 1011
FokI GGATG 2 cut(s) 19, 151
FspBI CTAG 1 cut(s) 89
GlaI GCGC 2 cut(s) 77, 191
GsuI CTGGAG 1 cut(s) 1154
HaeIII GGCC 3 cut(s) 133, 1139, 1158
HapII CCGG 1 cut(s) 178
HgaI GACGC 2 cut(s) 824, 1140
HhaI GCGC 2 cut(s) 78, 192
Hin1I GRCGYC 1 cut(s) 1151
Hin1II CATG 4 cut(s) 283, 814, 929, 1038
Hin6I GCGC 2 cut(s) 76, 190
HinP1I GCGC 2 cut(s) 76, 190
HincII GTYRAC 1 cut(s) 25
HindII GTYRAC 1 cut(s) 25
HindIII AAGCTT 1 cut(s) 344
HinfI GANTC 3 cut(s) 156, 791, 1124
HpaII CCGG 1 cut(s) 178
HphI GGTGA 2 cut(s) 512, 557
Hpy166II GTNNAC 2 cut(s) 25, 367
Hpy188I TCNGA 6 cut(s) 109, 310, 387, 398, 796, 1016
Hpy188III TCNNGA 6 cut(s) 208, 263, 350, 460, 677, 1128
Hpy8I GTNNAC 2 cut(s) 25, 367
Hpy99I CGWCG 3 cut(s) 130, 278, 840
HpyAV CCTTC 6 cut(s) 108, 112, 196, 518, 779, 884
HpyCH4III ACNGT 2 cut(s) 371, 610
HpyCH4IV ACGT 2 cut(s) 823, 1042
HpyCH4V TGCA 9 cut(s) 8, 151, 326, 367, 446, 614, 827, 857, 965
HpyF3I CTNAG 2 cut(s) 496, 1160
HpySE526I ACGT 2 cut(s) 823, 1042
Hsp92I GRCGYC 1 cut(s) 1151
Hsp92II CATG 4 cut(s) 283, 814, 929, 1038
HspAI GCGC 2 cut(s) 76, 190
Ksp22I TGATCA 1 cut(s) 1011
Kzo9I GATC 6 cut(s) 39, 182, 328, 719, 865, 1011
LguI GCTCTTC 1 cut(s) 679
LmnI GCTCC 2 cut(s) 87, 654
Lsp1109I GCAGC 5 cut(s) 20, 158, 310, 685, 961
LweI GCATC 3 cut(s) 41, 739, 974
MaeI CTAG 1 cut(s) 89
MaeII ACGT 2 cut(s) 823, 1042
MaeIII GTNAC 1 cut(s) 895
MalI GATC 6 cut(s) 41, 184, 330, 721, 867, 1013
MboI GATC 6 cut(s) 39, 182, 328, 719, 865, 1011
MboII GAAGA 7 cut(s) 659, 696, 727, 764, 767, 960, 1013
MflI RGATCY 2 cut(s) 182, 719
MhlI GDGCHC 1 cut(s) 369
MluCI AATT 9 cut(s) 291, 360, 409, 511, 519, 538, 598, 806, 859
MlyI GAGTC 1 cut(s) 785
Mph1103I ATGCAT 1 cut(s) 967
MroXI GAANNNNTTC 2 cut(s) 477, 688
MseI TTAA 3 cut(s) 518, 786, 831
MslI CAYNNNNRTG 1 cut(s) 960
MspA1I CMGCKG 1 cut(s) 974
MspI CCGG 1 cut(s) 178
MspR9I CCNGG 3 cut(s) 136, 179, 194
MvaI CCWGG 2 cut(s) 136, 194
MvnI CGCG 2 cut(s) 76, 190
NciI CCSGG 1 cut(s) 179
NcoI CCATGG 1 cut(s) 1034
NdeI CATATG 1 cut(s) 847
NdeII GATC 6 cut(s) 39, 182, 328, 719, 865, 1011
NlaIII CATG 4 cut(s) 283, 814, 929, 1038
NlaIV GGNNCC 1 cut(s) 184
NsiI ATGCAT 1 cut(s) 967
NspI RCATGY 1 cut(s) 929
PceI AGGCCT 1 cut(s) 1139
PciI ACATGT 1 cut(s) 925
PciSI GCTCTTC 1 cut(s) 679
PcsI WCGNNNNNNNCGW 1 cut(s) 261
PdmI GAANNNNTTC 2 cut(s) 477, 688
PfeI GAWTC 2 cut(s) 156, 1124
PflMI CCANNNNNTGG 1 cut(s) 626
PleI GAGTC 1 cut(s) 785
PpsI GAGTC 1 cut(s) 785
Ppu21I YACGTR 1 cut(s) 824
PscI ACATGT 1 cut(s) 925
PsiI TTATAA 1 cut(s) 605
Psp6I CCWGG 2 cut(s) 134, 192
PspGI CCWGG 2 cut(s) 134, 192
PspN4I GGNNCC 1 cut(s) 184
PspPI GGNCC 2 cut(s) 132, 882
PstI CTGCAG 1 cut(s) 328
PstNI CAGNNNCTG 2 cut(s) 199, 977
PsuI RGATCY 2 cut(s) 182, 719
PvuII CAGCTG 1 cut(s) 974
RsaI GTAC 3 cut(s) 470, 574, 924
RsaNI GTAC 3 cut(s) 469, 573, 923
RseI CAYNNNNRTG 1 cut(s) 960
SapI GCTCTTC 1 cut(s) 679
SaqAI TTAA 3 cut(s) 518, 786, 831
Sau3AI GATC 6 cut(s) 39, 182, 328, 719, 865, 1011
Sau96I GGNCC 2 cut(s) 132, 882
SchI GAGTC 1 cut(s) 785
ScrFI CCNGG 3 cut(s) 136, 179, 194
SduI GDGCHC 1 cut(s) 369
SfaNI GCATC 3 cut(s) 41, 739, 974
SfcI CTRYAG 1 cut(s) 324
SinI GGWCC 1 cut(s) 882
SmiMI CAYNNNNRTG 1 cut(s) 960
SmlI CTYRAG 2 cut(s) 631, 693
SmoI CTYRAG 2 cut(s) 631, 693
Sse9I AATT 9 cut(s) 291, 360, 409, 511, 519, 538, 598, 806, 859
SseBI AGGCCT 1 cut(s) 1139
SspMI CTAG 1 cut(s) 89
StuI AGGCCT 1 cut(s) 1139
StyD4I CCNGG 3 cut(s) 134, 177, 192
StyI CCWWGG 4 cut(s) 88, 113, 568, 1034
TaaI ACNGT 2 cut(s) 371, 610
TaiI ACGT 2 cut(s) 826, 1045
TaqI TCGA 3 cut(s) 207, 993, 1180
TasI AATT 9 cut(s) 291, 360, 409, 511, 519, 538, 598, 806, 859
TatI WGTACW 1 cut(s) 922
TauI GCSGC 6 cut(s) 72, 222, 225, 242, 245, 248
TfiI GAWTC 2 cut(s) 156, 1124
Tru1I TTAA 3 cut(s) 518, 786, 831
Tru9I TTAA 3 cut(s) 518, 786, 831
TscAI CASTG 2 cut(s) 307, 884
TseI GCWGC 5 cut(s) 8, 171, 323, 698, 974
TspDTI ATGAA 3 cut(s) 611, 799, 944
TspRI CASTG 2 cut(s) 307, 884
Van91I CCANNNNNTGG 1 cut(s) 626
VneI GTGCAC 1 cut(s) 365
VpaK11BI GGWCC 1 cut(s) 882
XagI CCTNNNNNAGG 1 cut(s) 567
XapI RAATTY 4 cut(s) 409, 598, 806, 859
XceI RCATGY 1 cut(s) 929
XmaJI CCTAGG 1 cut(s) 88
XmnI GAANNNNTTC 2 cut(s) 477, 688
XspI CTAG 1 cut(s) 89
Zsp2I ATGCAT 1 cut(s) 967
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.