Rh4DG112100

Putative serine esterase (DUF676)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0007465
Physical Location & Seq
Forward (+)
17594796 .. 17602346
7551 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG112100.1

Sequence Viewer

Length: 1518 bp
ATGATTGCAGCCACTTCTCCTGTCAACTCCGCTTCCCCGATCCCCGCCCCGAACCCGACCCGAACCCGCCGCCCGCGCCAGCTCCGCCTAGGGTTTCCTTCCTTCTCCGAGCCAAGGCTCTGTGCGACGCGGCCCAGGATGGACTTCCTGCACAGAATCGTAGGCGGAGGCTGCTTCCGGGGATCCACGCGCCAGGGGCTGAAGGTCGAGATTGATAGCGGCGGCGAGGATATCTTCGTTGCCGCCGCCAACAACGCCGTTCCCGAACATCTCGTCGTCATGGTCAATGGAATTATCGGCAGTGCTTCAGATTGGAGATATGCTGCAGATCAGTTTGTGAAAAAGCTTCCTGATAAAGTAATTGTGCACCGCAGTGAATGTAACTCTTCTAGATTGACATTTGATGGAGTTGACTTGATGGGTGAGAGGCTAGCTAAGGAGGTATTAGATGTTGTCAGAAAAAGGTCAGAGGTGCAGAAAATTTCCTTTGTCGCTCACTCTTTGGGGGGGTTGGTTGCAAGGTATGCTATCGGGATGCTTTACGAACCTTTTCCAAAACCCGAACCCTCAGGTGATAATAGTAATTGCATAATTGAAGGGAATATAAATAATTTGACACAATCCCTTGACCAGCCTCACCAAGGTACGATTGCTGGATTACAAGCTATGAATTTTATAACAGTTGCAACACCACATCTTGGCTCAAGGGGAAATAAACAGCTCCCATTTCTCTGTGGTCTTCCCTTTCTTGAAAGAAGAGCTTCTCAAGCTGCCCACTTTATTGTGGGGAGATCTGGGAAGCATCTCTTCCTAACAGATAAAGACGATGGCAAACCTCCCCTTCTTCTTCGAATGGTTAACGACTCTGATGACCTGAAATTCATGTCAGCATTACGTGCATTTAAGCGTCGTGTGGCATATGCTAATGCAAATTTTGATCATATGGTTGGGTGGAGAACTTCATCAATTCGACGTCAACATGAACTTCCGAAGTCCAATCTACTTGTAACAGATGAAAAATATCCACATATTGTACATGTGAAGCAAGAAACTTTAGATGATATTCATAATAATGCATCTTCAGCTGCTGAAAACCAAAATATCGACTTGGAAGAGGAGATGATCAGAGGTCTCACTCAAATACCATGGGAACGTGTAGATGTCAGCTTTCAAAAGAGCCGACAAAGATACATTGCTCATAATACCATTCAGGCAAGTTTGGTTATATTGCCATGGATTTTATATGATTTTGCATCAAACTATAAATTTTATGAATACCACCTAAAATTTTGCCTGCGTCAGTTTGACTCGTCCTGTCTCCTGATCTTCAAAGAGGAAACAAAAATGGTTCTTGTTGTCAAATCCATAATATTTTCTGTTAAAGTAACAATGGACCAAGTTTATAGTATACAGGACTGTTTTGTTTTTGGTTTTTTGTTTTATTGTTTGTTTGTTTTTATTTATTTATTTATTAAATTTTTTTTTAAGGATATTGTTGTTTTACATCTTGAAGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF676

Protein Analysis

505

Amino Acids

57.28

Weight (kDa)

8.91

Isoelectric Point (pI)

47.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF676 PF05057 88 - 320 1e-68 Putative serine esterase (DUF676)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 677
AatII GACGTC 1 cut(s) 976
AccB7I CCANNNNNTGG 1 cut(s) 698
AccI GTMKAC 1 cut(s) 1408
AccII CGCG 3 cut(s) 76, 130, 190
AclWI GGATC 3 cut(s) 34, 177, 190
AcsI RAATTY 7 cut(s) 480, 670, 878, 931, 1265, 1286, 1475
AcuI CTGAAG 3 cut(s) 221, 291, 1065
AcyI GRCGYC 1 cut(s) 973
AfaI GTAC 2 cut(s) 646, 1035
AfiI CCNNNNNNNGG 3 cut(s) 114, 641, 698
AflIII ACRYGT 2 cut(s) 1036, 1153
AgsI TTSAA 5 cut(s) 596, 752, 1172, 1330, 1511
AjnI CCWGG 2 cut(s) 134, 192
AleI CACNNNNGTG 1 cut(s) 372
AluBI AGCT 9 cut(s) 82, 346, 434, 665, 721, 761, 770, 1085, 1167
AluI AGCT 9 cut(s) 82, 346, 434, 665, 721, 761, 770, 1085, 1167
Alw21I GWGCWC 1 cut(s) 369
Alw26I GTCTC 2 cut(s) 1136, 1322
Alw44I GTGCAC 1 cut(s) 365
AlwI GGATC 3 cut(s) 34, 177, 190
AlwNI CAGNNNCTG 2 cut(s) 199, 1088
AoxI GGCC 1 cut(s) 131
ApaLI GTGCAC 1 cut(s) 365
ApeKI GCWGC 5 cut(s) 8, 171, 323, 770, 1085
ApoI RAATTY 7 cut(s) 480, 670, 878, 931, 1265, 1286, 1475
ArsI GACNNNNNNTTYG 2 cut(s) 258, 290
Asp700I GAANNNNTTC 2 cut(s) 549, 760
AspA2I CCTAGG 1 cut(s) 88
AspLEI GCGC 2 cut(s) 78, 192
AspS9I GGNCC 2 cut(s) 132, 1393
AsuC2I CCSGG 1 cut(s) 179
AsuHPI GGTGA 3 cut(s) 434, 584, 629
AsuII TTCGAA 1 cut(s) 850
AsuNHI GCTAGC 1 cut(s) 430
AvaII GGWCC 1 cut(s) 1393
AvrII CCTAGG 1 cut(s) 88
AxyI CCTNAGG 1 cut(s) 568
BaeGI GKGCMC 1 cut(s) 369
BamHI GGATCC 1 cut(s) 182
BbsI GAAGAC 1 cut(s) 731
Bbv12I GWGCWC 1 cut(s) 369
BbvI GCAGC 5 cut(s) 20, 158, 310, 757, 1072
BccI CCATC 4 cut(s) 133, 398, 412, 821
BceAI ACGGC 1 cut(s) 242
BciT130I CCWGG 2 cut(s) 136, 194
BclI TGATCA 2 cut(s) 937, 1122
BcnI CCSGG 1 cut(s) 179
BcoDI GTCTC 2 cut(s) 1136, 1322
BfaI CTAG 3 cut(s) 89, 390, 431
BfmI CTRYAG 1 cut(s) 324
BglII AGATCT 1 cut(s) 791
BlnI CCTAGG 1 cut(s) 88
Bme1390I CCNGG 3 cut(s) 136, 179, 194
Bme18I GGWCC 1 cut(s) 1393
BmgT120I GGNCC 2 cut(s) 132, 1393
BmiI GGNNCC 1 cut(s) 184
BmrFI CCNGG 3 cut(s) 136, 179, 194
BmsI GCATC 4 cut(s) 525, 811, 1085, 1262
BmtI GCTAGC 1 cut(s) 434
BpiI GAAGAC 1 cut(s) 731
Bpu10I CCTNAGC 1 cut(s) 435
Bpu14I TTCGAA 1 cut(s) 850
BpuEI CTTGAG 2 cut(s) 688, 750
BpuMI CCSGG 1 cut(s) 179
BsaAI YACGTR 1 cut(s) 896
BsaHI GRCGYC 1 cut(s) 973
BsaI GGTCTC 1 cut(s) 1136
BsaJI CCNNGG 8 cut(s) 88, 113, 134, 178, 193, 640, 1145, 1232
Bsc4I CCNNNNNNNGG 3 cut(s) 114, 641, 698
Bse21I CCTNAGG 1 cut(s) 568
Bse3DI GCAATG 1 cut(s) 1191
BseBI CCWGG 2 cut(s) 136, 194
BseDI CCNNGG 8 cut(s) 88, 113, 134, 178, 193, 640, 1145, 1232
BseGI GGATG 2 cut(s) 144, 540
BseLI CCNNNNNNNGG 3 cut(s) 114, 641, 698
BseMI GCAATG 1 cut(s) 1191
BseMII CTCAG 1 cut(s) 582
BseRI GAGGAG 1 cut(s) 1130
BseSI GKGCMC 1 cut(s) 369
BseXI GCAGC 5 cut(s) 20, 158, 310, 757, 1072
BsgI GTGCAG 2 cut(s) 134, 494
Bsh1236I CGCG 3 cut(s) 76, 130, 190
BshFI GGCC 1 cut(s) 133
BsiHKAI GWGCWC 1 cut(s) 369
BsiSI CCGG 1 cut(s) 178
BslI CCNNNNNNNGG 3 cut(s) 114, 641, 698
BsmAI GTCTC 2 cut(s) 1136, 1322
BsnI GGCC 1 cut(s) 133
Bso31I GGTCTC 1 cut(s) 1136
Bsp119I TTCGAA 1 cut(s) 850
Bsp1286I GDGCHC 1 cut(s) 369
Bsp1407I TGTACA 1 cut(s) 1033
Bsp143I GATC 7 cut(s) 39, 182, 328, 791, 937, 1122, 1323
Bsp19I CCATGG 2 cut(s) 1145, 1232
BspANI GGCC 1 cut(s) 133
BspCNI CTCAG 1 cut(s) 581
BspFNI CGCG 3 cut(s) 76, 130, 190
BspLI GGNNCC 1 cut(s) 184
BspMAI CTGCAG 1 cut(s) 328
BspOI GCTAGC 1 cut(s) 434
BspPI GGATC 3 cut(s) 34, 177, 190
BspQI GCTCTTC 1 cut(s) 751
BspT104I TTCGAA 1 cut(s) 850
BspTNI GGTCTC 1 cut(s) 1136
BsrDI GCAATG 1 cut(s) 1191
BsrGI TGTACA 1 cut(s) 1033
BssECI CCNNGG 8 cut(s) 88, 113, 134, 178, 193, 640, 1145, 1232
BssMI GATC 7 cut(s) 39, 182, 328, 791, 937, 1122, 1323
BssNAI GTATAC 1 cut(s) 1409
BssNI GRCGYC 1 cut(s) 973
BssT1I CCWWGG 5 cut(s) 88, 113, 640, 1145, 1232
Bst1107I GTATAC 1 cut(s) 1409
Bst2UI CCWGG 2 cut(s) 136, 194
Bst4CI ACNGT 2 cut(s) 682, 1418
Bst6I CTCTTC 4 cut(s) 391, 751, 812, 1107
BstACI GRCGYC 1 cut(s) 973
BstAPI GCANNNNNTGC 2 cut(s) 524, 896
BstAUI TGTACA 1 cut(s) 1033
BstBAI YACGTR 1 cut(s) 896
BstBI TTCGAA 1 cut(s) 850
BstC8I GCNNGC 4 cut(s) 74, 80, 432, 1295
BstDEI CTNAG 2 cut(s) 435, 568
BstDSI CCRYGG 2 cut(s) 1145, 1232
BstENI CCTNNNNNAGG 1 cut(s) 639
BstF5I GGATG 2 cut(s) 144, 540
BstFNI CGCG 3 cut(s) 76, 130, 190
BstHHI GCGC 2 cut(s) 78, 192
BstKTI GATC 7 cut(s) 42, 185, 331, 794, 940, 1125, 1326
BstMAI GTCTC 2 cut(s) 1136, 1322
BstMBI GATC 7 cut(s) 39, 182, 328, 791, 937, 1122, 1323
BstMWI GCNNNNNNNGC 9 cut(s) 75, 84, 171, 196, 254, 524, 767, 896, 1082
BstNI CCWGG 2 cut(s) 136, 194
BstNSI RCATGY 1 cut(s) 1040
BstSCI CCNGG 3 cut(s) 134, 177, 192
BstSFI CTRYAG 1 cut(s) 324
BstSLI GKGCMC 1 cut(s) 369
BstUI CGCG 3 cut(s) 76, 130, 190
BstV1I GCAGC 5 cut(s) 20, 158, 310, 757, 1072
BstV2I GAAGAC 1 cut(s) 731
BstX2I RGATCY 2 cut(s) 182, 791
BstYI RGATCY 2 cut(s) 182, 791
BstZ17I GTATAC 1 cut(s) 1409
Bsu36I CCTNAGG 1 cut(s) 568
BsuRI GGCC 1 cut(s) 133
BtgI CCRYGG 2 cut(s) 1145, 1232
BtsCI GGATG 2 cut(s) 144, 540
BtsI GCAGTG 2 cut(s) 307, 379
BtsIMutI CAGTG 2 cut(s) 307, 379
Cac8I GCNNGC 4 cut(s) 74, 80, 432, 1295
CaiI CAGNNNCTG 2 cut(s) 199, 1088
CfoI GCGC 2 cut(s) 78, 192
Cfr13I GGNCC 2 cut(s) 132, 1393
CseI GACGC 3 cut(s) 136, 896, 1286
Csp6I GTAC 2 cut(s) 645, 1034
CviAII CATG 6 cut(s) 280, 883, 980, 1037, 1146, 1233
CviQI GTAC 2 cut(s) 645, 1034
DdeI CTNAG 2 cut(s) 435, 568
DpnI GATC 7 cut(s) 41, 184, 330, 793, 939, 1124, 1325
DpnII GATC 7 cut(s) 39, 182, 328, 791, 937, 1122, 1323
Eam1104I CTCTTC 4 cut(s) 391, 751, 812, 1107
EarI CTCTTC 4 cut(s) 391, 751, 812, 1107
EciI GGCGGA 2 cut(s) 74, 180
Eco130I CCWWGG 5 cut(s) 88, 113, 640, 1145, 1232
Eco31I GGTCTC 1 cut(s) 1136
Eco32I GATATC 1 cut(s) 232
Eco47I GGWCC 1 cut(s) 1393
Eco57I CTGAAG 3 cut(s) 221, 291, 1065
Eco81I CCTNAGG 1 cut(s) 568
EcoNI CCTNNNNNAGG 1 cut(s) 639
EcoRII CCWGG 2 cut(s) 134, 192
EcoRV GATATC 1 cut(s) 232
EcoT14I CCWWGG 5 cut(s) 88, 113, 640, 1145, 1232
EcoT22I ATGCAT 1 cut(s) 1078
ErhI CCWWGG 5 cut(s) 88, 113, 640, 1145, 1232
FaeI CATG 6 cut(s) 283, 886, 983, 1040, 1149, 1236
FalI AAGNNNNNCTT 4 cut(s) 745, 777, 791, 823
FatI CATG 6 cut(s) 279, 882, 979, 1036, 1145, 1232
FauI CCCGC 3 cut(s) 52, 74, 81
FauNDI CATATG 2 cut(s) 919, 942
FbaI TGATCA 2 cut(s) 937, 1122
FblI GTMKAC 1 cut(s) 1408
FokI GGATG 2 cut(s) 151, 547
FspBI CTAG 3 cut(s) 89, 390, 431
GlaI GCGC 2 cut(s) 77, 191
HaeIII GGCC 1 cut(s) 133
HapII CCGG 1 cut(s) 178
HgaI GACGC 3 cut(s) 136, 896, 1286
HhaI GCGC 2 cut(s) 78, 192
Hin1I GRCGYC 1 cut(s) 973
Hin1II CATG 6 cut(s) 283, 886, 983, 1040, 1149, 1236
Hin6I GCGC 2 cut(s) 76, 190
HinP1I GCGC 2 cut(s) 76, 190
HincII GTYRAC 4 cut(s) 25, 412, 859, 977
HindII GTYRAC 4 cut(s) 25, 412, 859, 977
HindIII AAGCTT 1 cut(s) 344
HinfI GANTC 3 cut(s) 156, 863, 1307
HpaI GTTAAC 1 cut(s) 859
HpaII CCGG 1 cut(s) 178
HphI GGTGA 3 cut(s) 434, 584, 629
Hpy166II GTNNAC 6 cut(s) 25, 367, 412, 859, 977, 1409
Hpy188I TCNGA 7 cut(s) 109, 310, 458, 469, 868, 990, 1127
Hpy188III TCNNGA 8 cut(s) 208, 263, 350, 390, 532, 749, 1321, 1508
Hpy8I GTNNAC 6 cut(s) 25, 367, 412, 859, 977, 1409
Hpy99I CGWCG 4 cut(s) 130, 278, 912, 975
HpyAV CCTTC 5 cut(s) 108, 112, 196, 590, 851
HpyCH4III ACNGT 2 cut(s) 682, 1418
HpyCH4IV ACGT 3 cut(s) 895, 973, 1153
HpyF10VI GCNNNNNNNGC 9 cut(s) 75, 84, 171, 196, 254, 524, 767, 896, 1082
HpyF3I CTNAG 2 cut(s) 435, 568
HpySE526I ACGT 3 cut(s) 895, 973, 1153
Hsp92I GRCGYC 1 cut(s) 973
Hsp92II CATG 6 cut(s) 283, 886, 983, 1040, 1149, 1236
HspAI GCGC 2 cut(s) 76, 190
Ksp22I TGATCA 2 cut(s) 937, 1122
KspAI GTTAAC 1 cut(s) 859
Kzo9I GATC 7 cut(s) 39, 182, 328, 791, 937, 1122, 1323
LguI GCTCTTC 1 cut(s) 751
LmnI GCTCC 2 cut(s) 87, 726
Lsp1109I GCAGC 5 cut(s) 20, 158, 310, 757, 1072
LweI GCATC 4 cut(s) 525, 811, 1085, 1262
MaeI CTAG 3 cut(s) 89, 390, 431
MaeII ACGT 3 cut(s) 895, 973, 1153
MaeIII GTNAC 3 cut(s) 380, 1006, 1384
MalI GATC 7 cut(s) 41, 184, 330, 793, 939, 1124, 1325
MboI GATC 7 cut(s) 39, 182, 328, 791, 937, 1122, 1323
MflI RGATCY 2 cut(s) 182, 791
MhlI GDGCHC 1 cut(s) 369
MlyI GAGTC 2 cut(s) 857, 1301
Mph1103I ATGCAT 1 cut(s) 1078
MroXI GAANNNNTTC 2 cut(s) 549, 760
MseI TTAA 5 cut(s) 858, 903, 1380, 1473, 1485
MslI CAYNNNNRTG 2 cut(s) 372, 1071
MspA1I CMGCKG 1 cut(s) 1085
MspI CCGG 1 cut(s) 178
MspR9I CCNGG 3 cut(s) 136, 179, 194
MvaI CCWGG 2 cut(s) 136, 194
MvnI CGCG 3 cut(s) 76, 130, 190
MwoI GCNNNNNNNGC 9 cut(s) 75, 84, 171, 196, 254, 524, 767, 896, 1082
NciI CCSGG 1 cut(s) 179
NcoI CCATGG 2 cut(s) 1145, 1232
NdeI CATATG 2 cut(s) 919, 942
NdeII GATC 7 cut(s) 39, 182, 328, 791, 937, 1122, 1323
NheI GCTAGC 1 cut(s) 430
NlaIII CATG 6 cut(s) 283, 886, 983, 1040, 1149, 1236
NlaIV GGNNCC 1 cut(s) 184
NsiI ATGCAT 1 cut(s) 1078
NspI RCATGY 1 cut(s) 1040
NspV TTCGAA 1 cut(s) 850
OliI CACNNNNGTG 1 cut(s) 372
PciI ACATGT 1 cut(s) 1036
PciSI GCTCTTC 1 cut(s) 751
PcsI WCGNNNNNNNCGW 1 cut(s) 261
PdmI GAANNNNTTC 2 cut(s) 549, 760
PfeI GAWTC 1 cut(s) 156
PflMI CCANNNNNTGG 1 cut(s) 698
PleI GAGTC 2 cut(s) 857, 1301
PpsI GAGTC 2 cut(s) 857, 1301
Ppu21I YACGTR 1 cut(s) 896
PscI ACATGT 1 cut(s) 1036
PsiI TTATAA 1 cut(s) 677
Psp6I CCWGG 2 cut(s) 134, 192
PspGI CCWGG 2 cut(s) 134, 192
PspN4I GGNNCC 1 cut(s) 184
PspPI GGNCC 2 cut(s) 132, 1393
PstI CTGCAG 1 cut(s) 328
PstNI CAGNNNCTG 2 cut(s) 199, 1088
PsuI RGATCY 2 cut(s) 182, 791
PvuII CAGCTG 1 cut(s) 1085
RsaI GTAC 2 cut(s) 646, 1035
RsaNI GTAC 2 cut(s) 645, 1034
RseI CAYNNNNRTG 2 cut(s) 372, 1071
SapI GCTCTTC 1 cut(s) 751
SaqAI TTAA 5 cut(s) 858, 903, 1380, 1473, 1485
Sau3AI GATC 7 cut(s) 39, 182, 328, 791, 937, 1122, 1323
Sau96I GGNCC 2 cut(s) 132, 1393
SchI GAGTC 2 cut(s) 857, 1301
ScrFI CCNGG 3 cut(s) 136, 179, 194
SduI GDGCHC 1 cut(s) 369
SfaNI GCATC 4 cut(s) 525, 811, 1085, 1262
SfcI CTRYAG 1 cut(s) 324
SfuI TTCGAA 1 cut(s) 850
SinI GGWCC 1 cut(s) 1393
SmiMI CAYNNNNRTG 2 cut(s) 372, 1071
SmlI CTYRAG 2 cut(s) 703, 765
SmoI CTYRAG 2 cut(s) 703, 765
SspI AATATT 1 cut(s) 1371
SspMI CTAG 3 cut(s) 89, 390, 431
StyD4I CCNGG 3 cut(s) 134, 177, 192
StyI CCWWGG 5 cut(s) 88, 113, 640, 1145, 1232
TaaI ACNGT 2 cut(s) 682, 1418
TaiI ACGT 3 cut(s) 898, 976, 1156
TaqI TCGA 4 cut(s) 207, 850, 970, 1104
TatI WGTACW 1 cut(s) 1033
TauI GCSGC 6 cut(s) 72, 133, 222, 225, 245, 248
TfiI GAWTC 1 cut(s) 156
Tru1I TTAA 5 cut(s) 858, 903, 1380, 1473, 1485
Tru9I TTAA 5 cut(s) 858, 903, 1380, 1473, 1485
TscAI CASTG 2 cut(s) 307, 379
TseI GCWGC 5 cut(s) 8, 171, 323, 770, 1085
TspDTI ATGAA 7 cut(s) 683, 871, 951, 996, 1029, 1055, 1287
TspRI CASTG 2 cut(s) 307, 379
Van91I CCANNNNNTGG 1 cut(s) 698
VneI GTGCAC 1 cut(s) 365
VpaK11BI GGWCC 1 cut(s) 1393
XagI CCTNNNNNAGG 1 cut(s) 639
XapI RAATTY 7 cut(s) 480, 670, 878, 931, 1265, 1286, 1475
XbaI TCTAGA 1 cut(s) 389
XceI RCATGY 1 cut(s) 1040
XmaJI CCTAGG 1 cut(s) 88
XmiI GTMKAC 1 cut(s) 1408
XmnI GAANNNNTTC 2 cut(s) 549, 760
XspI CTAG 3 cut(s) 89, 390, 431
ZraI GACGTC 1 cut(s) 974
Zsp2I ATGCAT 1 cut(s) 1078
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.