Rh4AG119400

Putative serine esterase (DUF676)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0007465
Physical Location & Seq
Forward (+)
25759329 .. 25766200
6872 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG119400.1

Sequence Viewer

Length: 939 bp
ATGATTGCAGCCACTTCCCCTGTCAACTCCGCATCCCCGATCCCCGCCCCGAACCCGACCCGAACCCGCCGCCCGCGCCAGCTCCGCCTAGGGTTTCCTTCCTTCTCCGAGCCGAGGCTCTGTGCGACGCGGCCCAGGATGGACTTCCTGCACAGAATCGTAGGCGGAGGCTGCTTCCGGGGATCCACGCGCCAGGGACTGAAGGTCGAGATTGATAGCGGCGGCGAGGATATCTTCGATGCCGCCGCCAACAACGCCGTTCCCGAACATCTCGTCGTCATGGTCAATGGAATTATCGGCAGTGCTTCAGATTGGAGATATGCTGCAGATCAGTTTGTGAAAAAGCTTCCTGATAAAGTAATTGTGCACCGCAGTGAATGTAACTCTTCTAGATTGACATTTGATGGAGTTGACTTGATGGGTGAGAGGCTAGCTGAGGAGGTATTAGCTGTTGTCAGAAAAAGGTCAGAGGTGCGGAAAATTTCCTTTGTCGCTCACTCTTTGGGGGGGTTGGTTGCAAGGTATGCTATCGGGAGGCTTTACGAACCTTTTCCAAAACCCGAACCCTCAGGTGATAATGGTAATTGCATAATTGAAGGGAATACAAATAATTTGACACAATCCCTTGACCAGCCTCACCAAGGTACGATTGCTGGATTACAAGCTATGAATTTTATAACGGTTGCAACACCACATCTTGGCTCAAGGGGAAATAAACAGCTCCCATTTCTCTGTGGTCTTCCCTTTCTTGAAAGAAGAGCTTCTCAAGCTGCCCACTTTATTGTGGGGAGATCTGGGAAGCATCTCTTCCTAACAGATAAAGACGATGGCAAACCTCCCCTTCTTCTTCGAATGGTTAATGACTCTGATGACCTGAAATTCATGTTAGTATTAATATCACTGCAAATTCTTATTATTTTCATATACATTGAATTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF676

Protein Analysis

312

Amino Acids

34.31

Weight (kDa)

9.16

Isoelectric Point (pI)

46.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF676 PF05057 88 - 296 3.9e-60 Putative serine esterase (DUF676)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 677, 937
AccB7I CCANNNNNTGG 1 cut(s) 698
AccII CGCG 3 cut(s) 76, 130, 190
AclWI GGATC 3 cut(s) 34, 177, 190
AcsI RAATTY 4 cut(s) 480, 670, 878, 906
AcuI CTGAAG 2 cut(s) 221, 291
AfaI GTAC 1 cut(s) 646
AfiI CCNNNNNNNGG 3 cut(s) 114, 641, 698
AgsI TTSAA 3 cut(s) 596, 752, 932
AhdI GACNNNNNGTC 1 cut(s) 203
AjnI CCWGG 2 cut(s) 134, 192
AleI CACNNNNGTG 1 cut(s) 372
AluBI AGCT 8 cut(s) 82, 346, 434, 449, 665, 721, 761, 770
AluI AGCT 8 cut(s) 82, 346, 434, 449, 665, 721, 761, 770
Alw21I GWGCWC 1 cut(s) 369
Alw44I GTGCAC 1 cut(s) 365
AlwI GGATC 3 cut(s) 34, 177, 190
AlwNI CAGNNNCTG 1 cut(s) 199
AoxI GGCC 1 cut(s) 131
ApaLI GTGCAC 1 cut(s) 365
ApeKI GCWGC 4 cut(s) 8, 171, 323, 770
ApoI RAATTY 4 cut(s) 480, 670, 878, 906
ArsI GACNNNNNNTTYG 2 cut(s) 258, 290
AseI ATTAAT 1 cut(s) 893
Asp700I GAANNNNTTC 2 cut(s) 549, 760
AspA2I CCTAGG 1 cut(s) 88
AspLEI GCGC 2 cut(s) 78, 192
AspS9I GGNCC 1 cut(s) 132
AsuC2I CCSGG 1 cut(s) 179
AsuHPI GGTGA 3 cut(s) 434, 584, 629
AsuII TTCGAA 1 cut(s) 850
AsuNHI GCTAGC 1 cut(s) 430
AvrII CCTAGG 1 cut(s) 88
AxyI CCTNAGG 1 cut(s) 568
BaeGI GKGCMC 1 cut(s) 369
BamHI GGATCC 1 cut(s) 182
BbsI GAAGAC 1 cut(s) 731
Bbv12I GWGCWC 1 cut(s) 369
BbvCI CCTCAGC 1 cut(s) 435
BbvI GCAGC 4 cut(s) 20, 158, 310, 757
BccI CCATC 4 cut(s) 133, 398, 412, 821
BceAI ACGGC 1 cut(s) 242
BciT130I CCWGG 2 cut(s) 136, 194
BcnI CCSGG 1 cut(s) 179
BfaI CTAG 3 cut(s) 89, 390, 431
BfmI CTRYAG 1 cut(s) 324
BglII AGATCT 1 cut(s) 791
BlnI CCTAGG 1 cut(s) 88
Bme1390I CCNGG 3 cut(s) 136, 179, 194
BmeRI GACNNNNNGTC 1 cut(s) 203
BmgT120I GGNCC 1 cut(s) 132
BmiI GGNNCC 1 cut(s) 184
BmrFI CCNGG 3 cut(s) 136, 179, 194
BmsI GCATC 3 cut(s) 41, 229, 811
BmtI GCTAGC 1 cut(s) 434
BpiI GAAGAC 1 cut(s) 731
Bpu10I CCTNAGC 1 cut(s) 435
Bpu14I TTCGAA 1 cut(s) 850
BpuEI CTTGAG 2 cut(s) 688, 750
BpuMI CCSGG 1 cut(s) 179
BsaJI CCNNGG 6 cut(s) 88, 113, 134, 178, 193, 640
Bsc4I CCNNNNNNNGG 3 cut(s) 114, 641, 698
Bse21I CCTNAGG 1 cut(s) 568
BseBI CCWGG 2 cut(s) 136, 194
BseDI CCNNGG 6 cut(s) 88, 113, 134, 178, 193, 640
BseGI GGATG 2 cut(s) 32, 144
BseLI CCNNNNNNNGG 3 cut(s) 114, 641, 698
BseMII CTCAG 2 cut(s) 426, 582
BseRI GAGGAG 1 cut(s) 452
BseSI GKGCMC 1 cut(s) 369
BseXI GCAGC 4 cut(s) 20, 158, 310, 757
BsgI GTGCAG 1 cut(s) 134
Bsh1236I CGCG 3 cut(s) 76, 130, 190
BshFI GGCC 1 cut(s) 133
BsiHKAI GWGCWC 1 cut(s) 369
BsiSI CCGG 1 cut(s) 178
BslFI GGGAC 1 cut(s) 210
BslI CCNNNNNNNGG 3 cut(s) 114, 641, 698
BsmFI GGGAC 1 cut(s) 210
BsnI GGCC 1 cut(s) 133
Bsp119I TTCGAA 1 cut(s) 850
Bsp1286I GDGCHC 1 cut(s) 369
Bsp143I GATC 4 cut(s) 39, 182, 328, 791
BspANI GGCC 1 cut(s) 133
BspCNI CTCAG 2 cut(s) 427, 581
BspFNI CGCG 3 cut(s) 76, 130, 190
BspLI GGNNCC 1 cut(s) 184
BspMAI CTGCAG 1 cut(s) 328
BspOI GCTAGC 1 cut(s) 434
BspPI GGATC 3 cut(s) 34, 177, 190
BspQI GCTCTTC 1 cut(s) 751
BspT104I TTCGAA 1 cut(s) 850
BssECI CCNNGG 6 cut(s) 88, 113, 134, 178, 193, 640
BssMI GATC 4 cut(s) 39, 182, 328, 791
BssT1I CCWWGG 2 cut(s) 88, 640
Bst2UI CCWGG 2 cut(s) 136, 194
Bst4CI ACNGT 1 cut(s) 682
Bst6I CTCTTC 3 cut(s) 391, 751, 812
BstAPI GCANNNNNTGC 1 cut(s) 524
BstBI TTCGAA 1 cut(s) 850
BstC8I GCNNGC 3 cut(s) 74, 80, 432
BstDEI CTNAG 2 cut(s) 435, 568
BstENI CCTNNNNNAGG 1 cut(s) 639
BstF5I GGATG 2 cut(s) 32, 144
BstFNI CGCG 3 cut(s) 76, 130, 190
BstHHI GCGC 2 cut(s) 78, 192
BstKTI GATC 4 cut(s) 42, 185, 331, 794
BstMBI GATC 4 cut(s) 39, 182, 328, 791
BstMWI GCNNNNNNNGC 6 cut(s) 75, 84, 171, 254, 524, 767
BstNI CCWGG 2 cut(s) 136, 194
BstSCI CCNGG 3 cut(s) 134, 177, 192
BstSFI CTRYAG 1 cut(s) 324
BstSLI GKGCMC 1 cut(s) 369
BstUI CGCG 3 cut(s) 76, 130, 190
BstV1I GCAGC 4 cut(s) 20, 158, 310, 757
BstV2I GAAGAC 1 cut(s) 731
BstX2I RGATCY 2 cut(s) 182, 791
BstYI RGATCY 2 cut(s) 182, 791
Bsu36I CCTNAGG 1 cut(s) 568
BsuRI GGCC 1 cut(s) 133
BtsCI GGATG 2 cut(s) 32, 144
BtsI GCAGTG 3 cut(s) 307, 379, 899
BtsIMutI CAGTG 3 cut(s) 307, 379, 899
Cac8I GCNNGC 3 cut(s) 74, 80, 432
CaiI CAGNNNCTG 1 cut(s) 199
CfoI GCGC 2 cut(s) 78, 192
Cfr13I GGNCC 1 cut(s) 132
CseI GACGC 1 cut(s) 136
Csp6I GTAC 1 cut(s) 645
CviAII CATG 2 cut(s) 280, 883
CviQI GTAC 1 cut(s) 645
DdeI CTNAG 2 cut(s) 435, 568
DpnI GATC 4 cut(s) 41, 184, 330, 793
DpnII GATC 4 cut(s) 39, 182, 328, 791
DriI GACNNNNNGTC 1 cut(s) 203
Eam1104I CTCTTC 3 cut(s) 391, 751, 812
Eam1105I GACNNNNNGTC 1 cut(s) 203
EarI CTCTTC 3 cut(s) 391, 751, 812
EciI GGCGGA 2 cut(s) 74, 180
Eco130I CCWWGG 2 cut(s) 88, 640
Eco32I GATATC 1 cut(s) 232
Eco57I CTGAAG 2 cut(s) 221, 291
Eco81I CCTNAGG 1 cut(s) 568
EcoNI CCTNNNNNAGG 1 cut(s) 639
EcoRII CCWGG 2 cut(s) 134, 192
EcoRV GATATC 1 cut(s) 232
EcoT14I CCWWGG 2 cut(s) 88, 640
ErhI CCWWGG 2 cut(s) 88, 640
FaeI CATG 2 cut(s) 283, 886
FalI AAGNNNNNCTT 4 cut(s) 745, 777, 791, 823
FaqI GGGAC 1 cut(s) 210
FatI CATG 2 cut(s) 279, 882
FauI CCCGC 3 cut(s) 52, 74, 81
FokI GGATG 2 cut(s) 19, 151
FspBI CTAG 3 cut(s) 89, 390, 431
GlaI GCGC 2 cut(s) 77, 191
HaeIII GGCC 1 cut(s) 133
HapII CCGG 1 cut(s) 178
HgaI GACGC 1 cut(s) 136
HhaI GCGC 2 cut(s) 78, 192
Hin1II CATG 2 cut(s) 283, 886
Hin6I GCGC 2 cut(s) 76, 190
HinP1I GCGC 2 cut(s) 76, 190
HincII GTYRAC 2 cut(s) 25, 412
HindII GTYRAC 2 cut(s) 25, 412
HindIII AAGCTT 1 cut(s) 344
HinfI GANTC 2 cut(s) 156, 863
HpaII CCGG 1 cut(s) 178
HphI GGTGA 3 cut(s) 434, 584, 629
Hpy166II GTNNAC 3 cut(s) 25, 367, 412
Hpy188I TCNGA 5 cut(s) 109, 310, 458, 469, 868
Hpy188III TCNNGA 6 cut(s) 208, 263, 350, 390, 532, 749
Hpy8I GTNNAC 3 cut(s) 25, 367, 412
Hpy99I CGWCG 2 cut(s) 130, 278
HpyAV CCTTC 5 cut(s) 108, 112, 196, 590, 851
HpyCH4III ACNGT 1 cut(s) 682
HpyCH4V TGCA 8 cut(s) 8, 151, 326, 367, 518, 588, 686, 904
HpyF10VI GCNNNNNNNGC 6 cut(s) 75, 84, 171, 254, 524, 767
HpyF3I CTNAG 2 cut(s) 435, 568
Hsp92II CATG 2 cut(s) 283, 886
HspAI GCGC 2 cut(s) 76, 190
Kzo9I GATC 4 cut(s) 39, 182, 328, 791
LguI GCTCTTC 1 cut(s) 751
LmnI GCTCC 2 cut(s) 87, 726
Lsp1109I GCAGC 4 cut(s) 20, 158, 310, 757
LweI GCATC 3 cut(s) 41, 229, 811
MaeI CTAG 3 cut(s) 89, 390, 431
MaeIII GTNAC 1 cut(s) 380
MalI GATC 4 cut(s) 41, 184, 330, 793
MboI GATC 4 cut(s) 39, 182, 328, 791
MboII GAAGA 7 cut(s) 226, 378, 731, 768, 799, 836, 839
MflI RGATCY 2 cut(s) 182, 791
MhlI GDGCHC 1 cut(s) 369
MlyI GAGTC 1 cut(s) 857
MroXI GAANNNNTTC 2 cut(s) 549, 760
MseI TTAA 2 cut(s) 858, 893
MslI CAYNNNNRTG 1 cut(s) 372
MspI CCGG 1 cut(s) 178
MspR9I CCNGG 3 cut(s) 136, 179, 194
MvaI CCWGG 2 cut(s) 136, 194
MvnI CGCG 3 cut(s) 76, 130, 190
MwoI GCNNNNNNNGC 6 cut(s) 75, 84, 171, 254, 524, 767
NciI CCSGG 1 cut(s) 179
NdeII GATC 4 cut(s) 39, 182, 328, 791
NheI GCTAGC 1 cut(s) 430
NlaIII CATG 2 cut(s) 283, 886
NlaIV GGNNCC 1 cut(s) 184
NmeAIII GCCGAG 1 cut(s) 138
NspV TTCGAA 1 cut(s) 850
OliI CACNNNNGTG 1 cut(s) 372
PciSI GCTCTTC 1 cut(s) 751
PcsI WCGNNNNNNNCGW 1 cut(s) 261
PdmI GAANNNNTTC 2 cut(s) 549, 760
PfeI GAWTC 1 cut(s) 156
PflMI CCANNNNNTGG 1 cut(s) 698
PleI GAGTC 1 cut(s) 857
PpsI GAGTC 1 cut(s) 857
PshBI ATTAAT 1 cut(s) 893
PsiI TTATAA 2 cut(s) 677, 937
Psp6I CCWGG 2 cut(s) 134, 192
PspGI CCWGG 2 cut(s) 134, 192
PspN4I GGNNCC 1 cut(s) 184
PspPI GGNCC 1 cut(s) 132
PstI CTGCAG 1 cut(s) 328
PstNI CAGNNNCTG 1 cut(s) 199
PsuI RGATCY 2 cut(s) 182, 791
RsaI GTAC 1 cut(s) 646
RsaNI GTAC 1 cut(s) 645
RseI CAYNNNNRTG 1 cut(s) 372
SapI GCTCTTC 1 cut(s) 751
SaqAI TTAA 2 cut(s) 858, 893
Sau3AI GATC 4 cut(s) 39, 182, 328, 791
Sau96I GGNCC 1 cut(s) 132
SchI GAGTC 1 cut(s) 857
ScrFI CCNGG 3 cut(s) 136, 179, 194
SduI GDGCHC 1 cut(s) 369
SfaNI GCATC 3 cut(s) 41, 229, 811
SfcI CTRYAG 1 cut(s) 324
SfuI TTCGAA 1 cut(s) 850
SmiMI CAYNNNNRTG 1 cut(s) 372
SmlI CTYRAG 2 cut(s) 703, 765
SmoI CTYRAG 2 cut(s) 703, 765
SspMI CTAG 3 cut(s) 89, 390, 431
StyD4I CCNGG 3 cut(s) 134, 177, 192
StyI CCWWGG 2 cut(s) 88, 640
TaaI ACNGT 1 cut(s) 682
TaqI TCGA 3 cut(s) 207, 237, 850
TauI GCSGC 6 cut(s) 72, 133, 222, 225, 245, 248
TfiI GAWTC 1 cut(s) 156
Tru1I TTAA 2 cut(s) 858, 893
Tru9I TTAA 2 cut(s) 858, 893
TscAI CASTG 3 cut(s) 307, 379, 906
TseI GCWGC 4 cut(s) 8, 171, 323, 770
TspDTI ATGAA 3 cut(s) 683, 871, 910
TspRI CASTG 3 cut(s) 307, 379, 906
Van91I CCANNNNNTGG 1 cut(s) 698
VneI GTGCAC 1 cut(s) 365
VspI ATTAAT 1 cut(s) 893
XagI CCTNNNNNAGG 1 cut(s) 639
XapI RAATTY 4 cut(s) 480, 670, 878, 906
XbaI TCTAGA 1 cut(s) 389
XmaJI CCTAGG 1 cut(s) 88
XmnI GAANNNNTTC 2 cut(s) 549, 760
XspI CTAG 3 cut(s) 89, 390, 431
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.