MD13G1265600.v1.1

Putative serine esterase (DUF676)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0007465
Physical Location & Seq
Reverse (-)
34277845 .. 34282562
4718 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1265600.v1.1.491

Sequence Viewer

Length: 1302 bp
ATGATCGCCGCCAATTCTCCGCTCAATTCCGCAATCCCCGTCCTCCGCACCGCCGACCCGACCCGAAAGCACTGCTCTTTCGAGCCCCTGTGCTCCAGCAACCACCTAAGTTTTAGCTCCTCCGAGCGTGGGTTGTTTATCACTCGGACCCGGATGGACTTGCTCCGCCGATTTGTCACCGGAGGATGCGTCCGTGACCCCGATCACCAGGGGATAAAGGTGGAGATCGATAGCGGCGGCAAAGATGTATTTGCCGCCGACCCCAAGGGCAGTGGCGTGCCGGAACATCTAGTCATAATGGTCAATGGAATCATCGGCAGTGCTTCAGACTGGAGATACGCCGCAGATCAGTTTGTGAAAAAGCTTCCTGATAAAGTAATTGTGCACCGTAGTGAATGTAACTCTTCAAGACTGACATTTGACGGTGTTGACTTGATGGGTGAGAGGCTAGCTGAAGAGGTATTAGCTGTTGTCAGAAGGAGGTCTGAGGTGCGGAAAATATCTTTTGTGGCTCATTCCTTAGGGGGGTTGGTTGCAAGGTATGCCGTCGGAAGGCTGTATGAACCTTTTCCAAAATCAGAACCCTCAGGTCAAAATGGTAATTGCTTAATTGAAGGGCATACAAATAAGTTGACACAATCCATTGACCAGCCTCACCATGGTACAATTGCTGGACTGGAAGCAATGAACTTTATAACGGTTGCAACACCACATCTTGGTTCAAGGGGAAATAAACAGCTCCCATTTCTCTGTGGTCTTCCTTTTCTCGAGAGAAGAGCTTCTCAAACTGCACACTTTATTGTTGGGAGATCTGGGAAGCATCTCTTCCTAACAGACGATGATGATGGCAGACCTCCTCTTCTTCTTCGAATGGTTAATGACTGTGATGACCTAAAATTCATATCGGCTTTGCGTGCATTTAAGCGCCGTGTAGCATATGCTAATGCAAATTATGATCATATGGTTGGATGGAGAACATCATCAATCCGGCGTCAAGATGAACTTCCAAAGTCCAATCTACTTGTAACCGATGAGAGATATCCACATATCGTATATATCAAGCGAGAAACTTTTGATGATATTCATATGAAAGCTTCTTCATCTGCTCATGACGTAAAAATAGACTTGGAAGAGGAGATGATTAGAGGTCTTACTCAGATACCTTGGGAACGTGTGGATGTCAGCTTTCAAAAAAGTACACAACGATACATTGCTCATAATACTATTCAGGTGAAGAGCTATTGGTTGAATTCTGATGGTGCGGATGTGGTTTTCCATATCATAGATTCCTTCCTTCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF676

Protein Analysis

434

Amino Acids

48.59

Weight (kDa)

8.4

Isoelectric Point (pI)

42.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF676 PF05057 94 - 326 4.3e-68 Putative serine esterase (DUF676)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 695
AccB7I CCANNNNNTGG 1 cut(s) 716
AccBSI CCGCTC 1 cut(s) 22
AcsI RAATTY 2 cut(s) 896, 1249
AcuI CTGAAG 2 cut(s) 309, 474
AcyI GRCGYC 1 cut(s) 991
AfaI GTAC 2 cut(s) 664, 1198
AfiI CCNNNNNNNGG 5 cut(s) 129, 525, 552, 659, 716
AflIII ACRYGT 1 cut(s) 1171
AgsI TTSAA 5 cut(s) 408, 614, 723, 1190, 1249
AjnI CCWGG 1 cut(s) 207
AleI CACNNNNGTG 1 cut(s) 390
AluBI AGCT 9 cut(s) 117, 364, 452, 467, 739, 779, 1094, 1185, 1239
AluI AGCT 9 cut(s) 117, 364, 452, 467, 739, 779, 1094, 1185, 1239
Alw21I GWGCWC 2 cut(s) 95, 387
Alw44I GTGCAC 1 cut(s) 383
Ama87I CYCGRG 1 cut(s) 767
ApaLI GTGCAC 1 cut(s) 383
ApoI RAATTY 2 cut(s) 896, 1249
Asp700I GAANNNNTTC 2 cut(s) 567, 778
AspLEI GCGC 1 cut(s) 927
AspS9I GGNCC 1 cut(s) 147
AsuC2I CCSGG 1 cut(s) 151
AsuHPI GGTGA 5 cut(s) 169, 197, 452, 647, 1243
AsuII TTCGAA 1 cut(s) 868
AsuNHI GCTAGC 1 cut(s) 448
AvaI CYCGRG 1 cut(s) 767
AvaII GGWCC 1 cut(s) 147
AxyI CCTNAGG 2 cut(s) 520, 586
BaeGI GKGCMC 1 cut(s) 387
BanII GRGCYC 1 cut(s) 87
BbsI GAAGAC 1 cut(s) 749
Bbv12I GWGCWC 2 cut(s) 95, 387
BccI CCATC 5 cut(s) 148, 430, 839, 963, 1250
BceAI ACGGC 2 cut(s) 530, 912
BcgI CGANNNNNNTGC 2 cut(s) 54, 88
BciT130I CCWGG 1 cut(s) 209
BclI TGATCA 1 cut(s) 955
BcnI CCSGG 1 cut(s) 151
BfaI CTAG 2 cut(s) 290, 449
BfoI RGCGCY 1 cut(s) 928
BglII AGATCT 1 cut(s) 809
BisI GCNGC 5 cut(s) 9, 235, 238, 255, 342
BlsI GCNGC 5 cut(s) 10, 236, 239, 256, 343
Bme1390I CCNGG 2 cut(s) 151, 209
Bme18I GGWCC 1 cut(s) 147
BmeT110I CYCGRG 1 cut(s) 767
BmgT120I GGNCC 1 cut(s) 147
BmiI GGNNCC 1 cut(s) 149
BmrFI CCNGG 2 cut(s) 151, 209
BmsI GCATC 2 cut(s) 176, 829
BmtI GCTAGC 1 cut(s) 452
BpiI GAAGAC 1 cut(s) 749
BpmI CTGGAG 2 cut(s) 79, 352
Bpu14I TTCGAA 1 cut(s) 868
BpuMI CCSGG 1 cut(s) 151
Bsa29I ATCGAT 1 cut(s) 228
BsaHI GRCGYC 1 cut(s) 991
BsaJI CCNNGG 4 cut(s) 208, 264, 658, 1163
BsaWI WCCGGW 1 cut(s) 179
BsaXI ACNNNNNCTCC 2 cut(s) 174, 204
Bsc4I CCNNNNNNNGG 5 cut(s) 129, 525, 552, 659, 716
Bse1I ACTGG 2 cut(s) 335, 681
Bse21I CCTNAGG 2 cut(s) 520, 586
Bse3DI GCAATG 2 cut(s) 690, 1209
BseBI CCWGG 1 cut(s) 209
BseCI ATCGAT 1 cut(s) 228
BseDI CCNNGG 4 cut(s) 208, 264, 658, 1163
BseGI GGATG 5 cut(s) 159, 191, 974, 1183, 1270
BseLI CCNNNNNNNGG 5 cut(s) 129, 525, 552, 659, 716
BseMI GCAATG 2 cut(s) 690, 1209
BseMII CTCAG 3 cut(s) 477, 600, 1169
BseNI ACTGG 2 cut(s) 335, 681
BseRI GAGGAG 3 cut(s) 109, 846, 1148
BseSI GKGCMC 1 cut(s) 387
BsgI GTGCAG 1 cut(s) 774
BshVI ATCGAT 1 cut(s) 228
BsiHKAI GWGCWC 2 cut(s) 95, 387
BsiHKCI CYCGRG 1 cut(s) 767
BsiSI CCGG 4 cut(s) 151, 180, 281, 988
BslI CCNNNNNNNGG 5 cut(s) 129, 525, 552, 659, 716
BsoBI CYCGRG 1 cut(s) 767
Bsp119I TTCGAA 1 cut(s) 868
Bsp1286I GDGCHC 3 cut(s) 87, 95, 387
Bsp143I GATC 6 cut(s) 3, 202, 225, 346, 809, 955
Bsp19I CCATGG 1 cut(s) 658
BspCNI CTCAG 3 cut(s) 478, 599, 1168
BspDI ATCGAT 1 cut(s) 228
BspHI TCATGA 1 cut(s) 1108
BspLI GGNNCC 1 cut(s) 149
BspOI GCTAGC 1 cut(s) 452
BspQI GCTCTTC 2 cut(s) 769, 1229
BspT104I TTCGAA 1 cut(s) 868
BsrBI CCGCTC 1 cut(s) 22
BsrDI GCAATG 2 cut(s) 690, 1209
BsrI ACTGG 2 cut(s) 335, 681
BssECI CCNNGG 4 cut(s) 208, 264, 658, 1163
BssMI GATC 6 cut(s) 3, 202, 225, 346, 809, 955
BssNI GRCGYC 1 cut(s) 991
BssT1I CCWWGG 3 cut(s) 264, 658, 1163
Bst2UI CCWGG 1 cut(s) 209
Bst4CI ACNGT 4 cut(s) 389, 425, 700, 884
Bst6I CTCTTC 7 cut(s) 409, 450, 769, 830, 864, 1125, 1229
BstACI GRCGYC 1 cut(s) 991
BstAPI GCANNNNNTGC 1 cut(s) 542
BstBI TTCGAA 1 cut(s) 868
BstC8I GCNNGC 3 cut(s) 278, 450, 915
BstDEI CTNAG 5 cut(s) 107, 486, 520, 586, 1155
BstDSI CCRYGG 1 cut(s) 658
BstF5I GGATG 5 cut(s) 159, 191, 974, 1183, 1270
BstH2I RGCGCY 1 cut(s) 928
BstHHI GCGC 1 cut(s) 927
BstKTI GATC 6 cut(s) 6, 205, 228, 349, 812, 958
BstMBI GATC 6 cut(s) 3, 202, 225, 346, 809, 955
BstMWI GCNNNNNNNGC 2 cut(s) 542, 914
BstNI CCWGG 1 cut(s) 209
BstSCI CCNGG 2 cut(s) 149, 207
BstSLI GKGCMC 1 cut(s) 387
BstV2I GAAGAC 1 cut(s) 749
BstX2I RGATCY 1 cut(s) 809
BstYI RGATCY 1 cut(s) 809
Bsu15I ATCGAT 1 cut(s) 228
Bsu36I CCTNAGG 2 cut(s) 520, 586
BsuTUI ATCGAT 1 cut(s) 228
BtgI CCRYGG 1 cut(s) 658
BtsCI GGATG 5 cut(s) 159, 191, 974, 1183, 1270
BtsI GCAGTG 3 cut(s) 70, 277, 325
BtsIMutI CAGTG 3 cut(s) 70, 277, 325
Cac8I GCNNGC 3 cut(s) 278, 450, 915
CciI TCATGA 1 cut(s) 1108
CfoI GCGC 1 cut(s) 927
Cfr13I GGNCC 1 cut(s) 147
ClaI ATCGAT 1 cut(s) 228
CseI GACGC 2 cut(s) 178, 980
Csp6I GTAC 2 cut(s) 663, 1197
CspCI CAANNNNNGTGG 2 cut(s) 253, 288
CviAII CATG 2 cut(s) 659, 1109
CviQI GTAC 2 cut(s) 663, 1197
DdeI CTNAG 5 cut(s) 107, 486, 520, 586, 1155
DpnI GATC 6 cut(s) 5, 204, 227, 348, 811, 957
DpnII GATC 6 cut(s) 3, 202, 225, 346, 809, 955
Eam1104I CTCTTC 7 cut(s) 409, 450, 769, 830, 864, 1125, 1229
EarI CTCTTC 7 cut(s) 409, 450, 769, 830, 864, 1125, 1229
EciI GGCGGA 1 cut(s) 155
Eco130I CCWWGG 3 cut(s) 264, 658, 1163
Eco24I GRGCYC 1 cut(s) 87
Eco32I GATATC 1 cut(s) 1040
Eco47I GGWCC 1 cut(s) 147
Eco57I CTGAAG 2 cut(s) 309, 474
Eco81I CCTNAGG 2 cut(s) 520, 586
Eco88I CYCGRG 1 cut(s) 767
EcoRI GAATTC 1 cut(s) 1249
EcoRII CCWGG 1 cut(s) 207
EcoRV GATATC 1 cut(s) 1040
EcoT14I CCWWGG 3 cut(s) 264, 658, 1163
EcoT38I GRGCYC 1 cut(s) 87
ErhI CCWWGG 3 cut(s) 264, 658, 1163
FaeI CATG 2 cut(s) 662, 1112
FalI AAGNNNNNCTT 4 cut(s) 809, 841, 987, 1019
FatI CATG 2 cut(s) 658, 1108
FauNDI CATATG 3 cut(s) 937, 960, 1086
FbaI TGATCA 1 cut(s) 955
Fnu4HI GCNGC 5 cut(s) 9, 235, 238, 255, 342
FokI GGATG 5 cut(s) 166, 198, 981, 1190, 1277
FriOI GRGCYC 1 cut(s) 87
Fsp4HI GCNGC 5 cut(s) 9, 235, 238, 255, 342
FspBI CTAG 2 cut(s) 290, 449
GlaI GCGC 1 cut(s) 926
GluI GCNGC 5 cut(s) 9, 235, 238, 255, 342
GsuI CTGGAG 2 cut(s) 79, 352
HaeII RGCGCY 1 cut(s) 928
HapII CCGG 4 cut(s) 151, 180, 281, 988
HgaI GACGC 2 cut(s) 178, 980
HhaI GCGC 1 cut(s) 927
Hin1I GRCGYC 1 cut(s) 991
Hin1II CATG 2 cut(s) 662, 1112
Hin6I GCGC 1 cut(s) 925
HinP1I GCGC 1 cut(s) 925
HincII GTYRAC 2 cut(s) 430, 633
HindII GTYRAC 2 cut(s) 430, 633
HindIII AAGCTT 2 cut(s) 362, 1092
HinfI GANTC 2 cut(s) 309, 1286
HpaII CCGG 4 cut(s) 151, 180, 281, 988
HphI GGTGA 5 cut(s) 169, 197, 452, 647, 1243
Hpy166II GTNNAC 4 cut(s) 385, 430, 633, 1199
Hpy188I TCNGA 9 cut(s) 124, 147, 328, 476, 487, 551, 580, 1158, 1255
Hpy188III TCNNGA 6 cut(s) 368, 408, 767, 769, 995, 1109
Hpy8I GTNNAC 4 cut(s) 385, 430, 633, 1199
Hpy99I CGWCG 1 cut(s) 551
HpyAV CCTTC 4 cut(s) 471, 546, 608, 1300
HpyCH4III ACNGT 4 cut(s) 389, 425, 700, 884
HpyCH4IV ACGT 2 cut(s) 1113, 1171
HpyCH4V TGCA 6 cut(s) 385, 536, 704, 791, 917, 947
HpyF10VI GCNNNNNNNGC 2 cut(s) 542, 914
HpyF3I CTNAG 5 cut(s) 107, 486, 520, 586, 1155
HpySE526I ACGT 2 cut(s) 1113, 1171
Hsp92I GRCGYC 1 cut(s) 991
Hsp92II CATG 2 cut(s) 662, 1112
HspAI GCGC 1 cut(s) 925
Ksp22I TGATCA 1 cut(s) 955
Kzo9I GATC 6 cut(s) 3, 202, 225, 346, 809, 955
LguI GCTCTTC 2 cut(s) 769, 1229
LmnI GCTCC 4 cut(s) 98, 122, 168, 744
LweI GCATC 2 cut(s) 176, 829
MaeI CTAG 2 cut(s) 290, 449
MaeII ACGT 2 cut(s) 1113, 1171
MaeIII GTNAC 4 cut(s) 175, 194, 398, 1024
MalI GATC 6 cut(s) 5, 204, 227, 348, 811, 957
MbiI CCGCTC 1 cut(s) 22
MboI GATC 6 cut(s) 3, 202, 225, 346, 809, 955
MfeI CAATTG 1 cut(s) 666
MflI RGATCY 1 cut(s) 809
MhlI GDGCHC 3 cut(s) 87, 95, 387
MluCI AATT 9 cut(s) 13, 25, 378, 601, 609, 666, 896, 949, 1249
MmeI TCCRAC 2 cut(s) 529, 946
MroXI GAANNNNTTC 2 cut(s) 567, 778
MseI TTAA 3 cut(s) 608, 876, 921
MslI CAYNNNNRTG 1 cut(s) 390
MspI CCGG 4 cut(s) 151, 180, 281, 988
MspR9I CCNGG 2 cut(s) 151, 209
MunI CAATTG 1 cut(s) 666
MvaI CCWGG 1 cut(s) 209
MwoI GCNNNNNNNGC 2 cut(s) 542, 914
NciI CCSGG 1 cut(s) 151
NcoI CCATGG 1 cut(s) 658
NdeI CATATG 3 cut(s) 937, 960, 1086
NdeII GATC 6 cut(s) 3, 202, 225, 346, 809, 955
NheI GCTAGC 1 cut(s) 448
NlaIII CATG 2 cut(s) 662, 1112
NlaIV GGNNCC 1 cut(s) 149
NmuCI GTSAC 2 cut(s) 175, 194
NspV TTCGAA 1 cut(s) 868
OliI CACNNNNGTG 1 cut(s) 390
PaeR7I CTCGAG 1 cut(s) 767
PagI TCATGA 1 cut(s) 1108
PciSI GCTCTTC 2 cut(s) 769, 1229
PdmI GAANNNNTTC 2 cut(s) 567, 778
PfeI GAWTC 2 cut(s) 309, 1286
PflMI CCANNNNNTGG 1 cut(s) 716
PkrI GCNGC 5 cut(s) 10, 236, 239, 256, 343
PsiI TTATAA 1 cut(s) 695
Psp6I CCWGG 1 cut(s) 207
PspGI CCWGG 1 cut(s) 207
PspN4I GGNNCC 1 cut(s) 149
PspPI GGNCC 1 cut(s) 147
PsuI RGATCY 1 cut(s) 809
RsaI GTAC 2 cut(s) 664, 1198
RsaNI GTAC 2 cut(s) 663, 1197
RseI CAYNNNNRTG 1 cut(s) 390
SapI GCTCTTC 2 cut(s) 769, 1229
SaqAI TTAA 3 cut(s) 608, 876, 921
SatI GCNGC 5 cut(s) 9, 235, 238, 255, 342
Sau3AI GATC 6 cut(s) 3, 202, 225, 346, 809, 955
Sau96I GGNCC 1 cut(s) 147
ScrFI CCNGG 2 cut(s) 151, 209
SduI GDGCHC 3 cut(s) 87, 95, 387
SfaNI GCATC 2 cut(s) 176, 829
Sfr274I CTCGAG 1 cut(s) 767
SfuI TTCGAA 1 cut(s) 868
SinI GGWCC 1 cut(s) 147
SlaI CTCGAG 1 cut(s) 767
SmiMI CAYNNNNRTG 1 cut(s) 390
SmlI CTYRAG 1 cut(s) 767
SmoI CTYRAG 1 cut(s) 767
Sse9I AATT 9 cut(s) 13, 25, 378, 601, 609, 666, 896, 949, 1249
SspMI CTAG 2 cut(s) 290, 449
StyD4I CCNGG 2 cut(s) 149, 207
StyI CCWWGG 3 cut(s) 264, 658, 1163
TaaI ACNGT 4 cut(s) 389, 425, 700, 884
TaiI ACGT 2 cut(s) 1116, 1174
TaqI TCGA 4 cut(s) 81, 228, 768, 868
TasI AATT 9 cut(s) 13, 25, 378, 601, 609, 666, 896, 949, 1249
TatI WGTACW 1 cut(s) 1196
TauI GCSGC 5 cut(s) 11, 237, 240, 257, 344
TfiI GAWTC 2 cut(s) 309, 1286
Tru1I TTAA 3 cut(s) 608, 876, 921
Tru9I TTAA 3 cut(s) 608, 876, 921
TscAI CASTG 3 cut(s) 77, 277, 325
TseFI GTSAC 2 cut(s) 175, 194
Tsp45I GTSAC 2 cut(s) 175, 194
TspDTI ATGAA 7 cut(s) 576, 701, 889, 1014, 1073, 1089, 1103
TspGWI ACGGA 1 cut(s) 182
TspRI CASTG 3 cut(s) 77, 277, 325
Van91I CCANNNNNTGG 1 cut(s) 716
VneI GTGCAC 1 cut(s) 383
VpaK11BI GGWCC 1 cut(s) 147
XapI RAATTY 2 cut(s) 896, 1249
XcmI CCANNNNNNNNNTGG 1 cut(s) 656
XhoI CTCGAG 1 cut(s) 767
XmnI GAANNNNTTC 2 cut(s) 567, 778
XspI CTAG 2 cut(s) 290, 449
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.