Rh4BG112400

Putative serine esterase (DUF676)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0007465
Physical Location & Seq
Forward (+)
19058695 .. 19064911
6217 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG112400.1

Sequence Viewer

Length: 1290 bp
ATGATTGCAGCCACTTCTCCTGTCAACTCCGCATCCCCGATCCCCGCCCCGAACCCGACCCGAACCCGCCGCCCGCGCCAGTTCCGCCTAGGGTTTCCTTCCTTCTCCGAGCCAAGGCTCTGTGCGACGCGGCCCAGGATGGACTTCCTGCACAGAATCGTAGGCGGAGGCTGCTTCCGGGGATCCACGCGCCAGGGACTGAAGGTCGAGATTGATAACGGCGGCGAGGATATCTTCGATGCCGCCGCCGCCGCCAACAACGCCGTTCCCGAACATCTCGTCGTCATGGTCAATGGAATTATCGGCAGTGCTTCAGATTGGAGATATGCTGCAGATCAGTTTGTGAAAAAGCTTCCTGATAAAGTAATTGTGCACCGCAGTGAATGTAACTCTTCTAGATTGACATTTGATGGAGTTGACTTGATGGGTGAGAGGCTAGCTGAGGAGGTATTAGCTGTTGTCAGAAAAAGGTCAGAGGTGCGGAAAATTTCCTTTGTCGCTCACTCTTTGGGGGGGTTGGTTGCAAGGTATGCTATCGGGAGGCTTTACGAACCTTTTCCAAAACCCGAACCCTCAGGTGAAAATGGTAATTGCATAATTGAAGGGAATACAAATAATTTGACACAATCCCTTGACCAGCCTCACCAAGGTACGATTGCTGGATTACAAGCTATGAATTTTATAACGGTTGCAACACCACATCTTGGCTCAAGGGGAAATAAACAGCTCCCATTTCTCTGTGGTCTTCCCTTTCTTGAAAGAAGAGCTTCTCAAGCTGCCCACTTTATTGTGGGGAGATCTGGGAAGCATCTCTTCCTAACAGATAAAGACAATGGCAAACCTCCCCTTCTTCTTCGAATGGTTAATGACTCTGATGACCTGAAATTCATGTCAGCATTACGTGCATTTAAGCGTCGTGTGGCATATGCTAATGCAAATTTTGATCATATGGTTGGGTGGAGAACTTCATCGATTCGACGTCAACATGAACTTCCGAAGTCCAATCTACTTGTAACAGATGAAAAATATCCACATATTGTACATGTGAAGCAAGAAACTTTACATGATATTCATAATAATGCATCTTCAGCTGCTGAAAACCAAAATATCGACTTGGAAGAGGAGATGATCAGAGGTCTCACTCAAATACCATGGGAACGTGTAGATGTCAGCTTTCAAAAGAGCCGACAAAGATACATTGCTCATAATACCATTCAGGTCAAAAGATATTGGTTGAATTCTGATGGTGCTGATGTGGTTTTCCATATCATAGATTACTTCCTTCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF676

Protein Analysis

429

Amino Acids

48.08

Weight (kDa)

9.33

Isoelectric Point (pI)

45.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF676 PF05057 90 - 322 3.3e-68 Putative serine esterase (DUF676)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 683
AatII GACGTC 1 cut(s) 982
AccB7I CCANNNNNTGG 1 cut(s) 704
AccII CGCG 3 cut(s) 76, 130, 190
AclWI GGATC 3 cut(s) 34, 177, 190
AcsI RAATTY 5 cut(s) 486, 676, 884, 937, 1237
AcuI CTGAAG 3 cut(s) 221, 297, 1071
AcyI GRCGYC 1 cut(s) 979
AfaI GTAC 2 cut(s) 652, 1041
AfiI CCNNNNNNNGG 3 cut(s) 114, 647, 704
AflIII ACRYGT 2 cut(s) 1042, 1159
AgsI TTSAA 4 cut(s) 602, 758, 1178, 1237
AhdI GACNNNNNGTC 1 cut(s) 203
AjnI CCWGG 2 cut(s) 134, 192
AleI CACNNNNGTG 1 cut(s) 378
AluBI AGCT 9 cut(s) 352, 440, 455, 671, 727, 767, 776, 1091, 1173
AluI AGCT 9 cut(s) 352, 440, 455, 671, 727, 767, 776, 1091, 1173
Alw21I GWGCWC 1 cut(s) 375
Alw26I GTCTC 1 cut(s) 1142
Alw44I GTGCAC 1 cut(s) 371
AlwI GGATC 3 cut(s) 34, 177, 190
AlwNI CAGNNNCTG 2 cut(s) 199, 1094
AoxI GGCC 1 cut(s) 131
ApaLI GTGCAC 1 cut(s) 371
ApeKI GCWGC 5 cut(s) 8, 171, 329, 776, 1091
ApoI RAATTY 5 cut(s) 486, 676, 884, 937, 1237
ArsI GACNNNNNNTTYG 2 cut(s) 264, 296
Asp700I GAANNNNTTC 2 cut(s) 555, 766
AspA2I CCTAGG 1 cut(s) 88
AspLEI GCGC 2 cut(s) 78, 192
AspS9I GGNCC 1 cut(s) 132
AsuC2I CCSGG 1 cut(s) 179
AsuHPI GGTGA 3 cut(s) 440, 590, 635
AsuII TTCGAA 1 cut(s) 856
AsuNHI GCTAGC 1 cut(s) 436
AvrII CCTAGG 1 cut(s) 88
AxyI CCTNAGG 1 cut(s) 574
BaeGI GKGCMC 1 cut(s) 375
BamHI GGATCC 1 cut(s) 182
BbsI GAAGAC 1 cut(s) 737
Bbv12I GWGCWC 1 cut(s) 375
BbvCI CCTCAGC 1 cut(s) 441
BbvI GCAGC 5 cut(s) 20, 158, 316, 763, 1078
BccI CCATC 4 cut(s) 133, 404, 418, 1238
BceAI ACGGC 2 cut(s) 235, 248
BciT130I CCWGG 2 cut(s) 136, 194
BclI TGATCA 2 cut(s) 943, 1128
BcnI CCSGG 1 cut(s) 179
BcoDI GTCTC 1 cut(s) 1142
BfaI CTAG 3 cut(s) 89, 396, 437
BfmI CTRYAG 1 cut(s) 330
BglII AGATCT 1 cut(s) 797
BlnI CCTAGG 1 cut(s) 88
Bme1390I CCNGG 3 cut(s) 136, 179, 194
BmeRI GACNNNNNGTC 1 cut(s) 203
BmgT120I GGNCC 1 cut(s) 132
BmiI GGNNCC 1 cut(s) 184
BmrFI CCNGG 3 cut(s) 136, 179, 194
BmsI GCATC 4 cut(s) 41, 229, 817, 1091
BmtI GCTAGC 1 cut(s) 440
BpiI GAAGAC 1 cut(s) 737
Bpu10I CCTNAGC 1 cut(s) 441
Bpu14I TTCGAA 1 cut(s) 856
BpuEI CTTGAG 2 cut(s) 694, 756
BpuMI CCSGG 1 cut(s) 179
Bsa29I ATCGAT 1 cut(s) 971
BsaAI YACGTR 1 cut(s) 902
BsaHI GRCGYC 1 cut(s) 979
BsaI GGTCTC 1 cut(s) 1142
BsaJI CCNNGG 7 cut(s) 88, 113, 134, 178, 193, 646, 1151
Bsc4I CCNNNNNNNGG 3 cut(s) 114, 647, 704
Bse1I ACTGG 1 cut(s) 79
Bse21I CCTNAGG 1 cut(s) 574
Bse3DI GCAATG 1 cut(s) 1197
BseBI CCWGG 2 cut(s) 136, 194
BseCI ATCGAT 1 cut(s) 971
BseDI CCNNGG 7 cut(s) 88, 113, 134, 178, 193, 646, 1151
BseGI GGATG 2 cut(s) 32, 144
BseLI CCNNNNNNNGG 3 cut(s) 114, 647, 704
BseMI GCAATG 1 cut(s) 1197
BseMII CTCAG 2 cut(s) 432, 588
BseNI ACTGG 1 cut(s) 79
BseRI GAGGAG 2 cut(s) 458, 1136
BseSI GKGCMC 1 cut(s) 375
BseXI GCAGC 5 cut(s) 20, 158, 316, 763, 1078
BsgI GTGCAG 1 cut(s) 134
Bsh1236I CGCG 3 cut(s) 76, 130, 190
BshFI GGCC 1 cut(s) 133
BshVI ATCGAT 1 cut(s) 971
BsiHKAI GWGCWC 1 cut(s) 375
BsiSI CCGG 1 cut(s) 178
BslFI GGGAC 1 cut(s) 210
BslI CCNNNNNNNGG 3 cut(s) 114, 647, 704
BsmAI GTCTC 1 cut(s) 1142
BsmFI GGGAC 1 cut(s) 210
BsnI GGCC 1 cut(s) 133
Bso31I GGTCTC 1 cut(s) 1142
Bsp119I TTCGAA 1 cut(s) 856
Bsp1286I GDGCHC 1 cut(s) 375
Bsp1407I TGTACA 1 cut(s) 1039
Bsp143I GATC 6 cut(s) 39, 182, 334, 797, 943, 1128
Bsp19I CCATGG 1 cut(s) 1151
BspANI GGCC 1 cut(s) 133
BspCNI CTCAG 2 cut(s) 433, 587
BspDI ATCGAT 1 cut(s) 971
BspFNI CGCG 3 cut(s) 76, 130, 190
BspLI GGNNCC 1 cut(s) 184
BspMAI CTGCAG 1 cut(s) 334
BspOI GCTAGC 1 cut(s) 440
BspPI GGATC 3 cut(s) 34, 177, 190
BspQI GCTCTTC 1 cut(s) 757
BspT104I TTCGAA 1 cut(s) 856
BspTNI GGTCTC 1 cut(s) 1142
BsrDI GCAATG 1 cut(s) 1197
BsrGI TGTACA 1 cut(s) 1039
BsrI ACTGG 1 cut(s) 79
BssECI CCNNGG 7 cut(s) 88, 113, 134, 178, 193, 646, 1151
BssMI GATC 6 cut(s) 39, 182, 334, 797, 943, 1128
BssNI GRCGYC 1 cut(s) 979
BssT1I CCWWGG 4 cut(s) 88, 113, 646, 1151
Bst2UI CCWGG 2 cut(s) 136, 194
Bst4CI ACNGT 1 cut(s) 688
Bst6I CTCTTC 4 cut(s) 397, 757, 818, 1113
BstACI GRCGYC 1 cut(s) 979
BstAPI GCANNNNNTGC 2 cut(s) 530, 902
BstAUI TGTACA 1 cut(s) 1039
BstBAI YACGTR 1 cut(s) 902
BstBI TTCGAA 1 cut(s) 856
BstC8I GCNNGC 2 cut(s) 74, 438
BstDEI CTNAG 2 cut(s) 441, 574
BstDSI CCRYGG 1 cut(s) 1151
BstENI CCTNNNNNAGG 1 cut(s) 645
BstF5I GGATG 2 cut(s) 32, 144
BstFNI CGCG 3 cut(s) 76, 130, 190
BstHHI GCGC 2 cut(s) 78, 192
BstKTI GATC 6 cut(s) 42, 185, 337, 800, 946, 1131
BstMAI GTCTC 1 cut(s) 1142
BstMBI GATC 6 cut(s) 39, 182, 334, 797, 943, 1128
BstNI CCWGG 2 cut(s) 136, 194
BstNSI RCATGY 1 cut(s) 1046
BstSCI CCNGG 3 cut(s) 134, 177, 192
BstSFI CTRYAG 1 cut(s) 330
BstSLI GKGCMC 1 cut(s) 375
BstUI CGCG 3 cut(s) 76, 130, 190
BstV1I GCAGC 5 cut(s) 20, 158, 316, 763, 1078
BstV2I GAAGAC 1 cut(s) 737
BstX2I RGATCY 2 cut(s) 182, 797
BstYI RGATCY 2 cut(s) 182, 797
Bsu15I ATCGAT 1 cut(s) 971
Bsu36I CCTNAGG 1 cut(s) 574
BsuRI GGCC 1 cut(s) 133
BsuTUI ATCGAT 1 cut(s) 971
BtgI CCRYGG 1 cut(s) 1151
BtsCI GGATG 2 cut(s) 32, 144
BtsI GCAGTG 2 cut(s) 313, 385
BtsIMutI CAGTG 2 cut(s) 313, 385
Cac8I GCNNGC 2 cut(s) 74, 438
CaiI CAGNNNCTG 2 cut(s) 199, 1094
CfoI GCGC 2 cut(s) 78, 192
Cfr13I GGNCC 1 cut(s) 132
ClaI ATCGAT 1 cut(s) 971
CseI GACGC 2 cut(s) 136, 902
Csp6I GTAC 2 cut(s) 651, 1040
CviAII CATG 6 cut(s) 286, 889, 986, 1043, 1064, 1152
CviQI GTAC 2 cut(s) 651, 1040
DdeI CTNAG 2 cut(s) 441, 574
DpnI GATC 6 cut(s) 41, 184, 336, 799, 945, 1130
DpnII GATC 6 cut(s) 39, 182, 334, 797, 943, 1128
DriI GACNNNNNGTC 1 cut(s) 203
Eam1104I CTCTTC 4 cut(s) 397, 757, 818, 1113
Eam1105I GACNNNNNGTC 1 cut(s) 203
EarI CTCTTC 4 cut(s) 397, 757, 818, 1113
EciI GGCGGA 2 cut(s) 74, 180
Eco130I CCWWGG 4 cut(s) 88, 113, 646, 1151
Eco31I GGTCTC 1 cut(s) 1142
Eco32I GATATC 1 cut(s) 232
Eco57I CTGAAG 3 cut(s) 221, 297, 1071
Eco81I CCTNAGG 1 cut(s) 574
EcoNI CCTNNNNNAGG 1 cut(s) 645
EcoRI GAATTC 1 cut(s) 1237
EcoRII CCWGG 2 cut(s) 134, 192
EcoRV GATATC 1 cut(s) 232
EcoT14I CCWWGG 4 cut(s) 88, 113, 646, 1151
EcoT22I ATGCAT 1 cut(s) 1084
ErhI CCWWGG 4 cut(s) 88, 113, 646, 1151
FaeI CATG 6 cut(s) 289, 892, 989, 1046, 1067, 1155
FalI AAGNNNNNCTT 4 cut(s) 751, 783, 797, 829
FaqI GGGAC 1 cut(s) 210
FatI CATG 6 cut(s) 285, 888, 985, 1042, 1063, 1151
FauI CCCGC 3 cut(s) 52, 74, 81
FauNDI CATATG 2 cut(s) 925, 948
FbaI TGATCA 2 cut(s) 943, 1128
FokI GGATG 2 cut(s) 19, 151
FspBI CTAG 3 cut(s) 89, 396, 437
GlaI GCGC 2 cut(s) 77, 191
HaeIII GGCC 1 cut(s) 133
HapII CCGG 1 cut(s) 178
HgaI GACGC 2 cut(s) 136, 902
HhaI GCGC 2 cut(s) 78, 192
Hin1I GRCGYC 1 cut(s) 979
Hin1II CATG 6 cut(s) 289, 892, 989, 1046, 1067, 1155
Hin6I GCGC 2 cut(s) 76, 190
HinP1I GCGC 2 cut(s) 76, 190
HincII GTYRAC 3 cut(s) 25, 418, 983
HindII GTYRAC 3 cut(s) 25, 418, 983
HindIII AAGCTT 1 cut(s) 350
HinfI GANTC 3 cut(s) 156, 869, 973
HpaII CCGG 1 cut(s) 178
HphI GGTGA 3 cut(s) 440, 590, 635
Hpy166II GTNNAC 4 cut(s) 25, 373, 418, 983
Hpy188I TCNGA 8 cut(s) 109, 316, 464, 475, 874, 996, 1133, 1243
Hpy188III TCNNGA 6 cut(s) 208, 269, 356, 396, 538, 755
Hpy8I GTNNAC 4 cut(s) 25, 373, 418, 983
Hpy99I CGWCG 4 cut(s) 130, 284, 918, 981
HpyAV CCTTC 5 cut(s) 108, 112, 196, 596, 857
HpyCH4III ACNGT 1 cut(s) 688
HpyCH4IV ACGT 3 cut(s) 901, 979, 1159
HpyF3I CTNAG 2 cut(s) 441, 574
HpySE526I ACGT 3 cut(s) 901, 979, 1159
Hsp92I GRCGYC 1 cut(s) 979
Hsp92II CATG 6 cut(s) 289, 892, 989, 1046, 1067, 1155
HspAI GCGC 2 cut(s) 76, 190
Ksp22I TGATCA 2 cut(s) 943, 1128
Kzo9I GATC 6 cut(s) 39, 182, 334, 797, 943, 1128
LguI GCTCTTC 1 cut(s) 757
LmnI GCTCC 1 cut(s) 732
Lsp1109I GCAGC 5 cut(s) 20, 158, 316, 763, 1078
LweI GCATC 4 cut(s) 41, 229, 817, 1091
MaeI CTAG 3 cut(s) 89, 396, 437
MaeII ACGT 3 cut(s) 901, 979, 1159
MaeIII GTNAC 2 cut(s) 386, 1012
MalI GATC 6 cut(s) 41, 184, 336, 799, 945, 1130
MboI GATC 6 cut(s) 39, 182, 334, 797, 943, 1128
MboII GAAGA 9 cut(s) 226, 384, 737, 774, 805, 842, 845, 1077, 1130
MflI RGATCY 2 cut(s) 182, 797
MhlI GDGCHC 1 cut(s) 375
MlyI GAGTC 1 cut(s) 863
Mph1103I ATGCAT 1 cut(s) 1084
MroXI GAANNNNTTC 2 cut(s) 555, 766
MseI TTAA 2 cut(s) 864, 909
MslI CAYNNNNRTG 2 cut(s) 378, 1077
MspA1I CMGCKG 1 cut(s) 1091
MspI CCGG 1 cut(s) 178
MspR9I CCNGG 3 cut(s) 136, 179, 194
MvaI CCWGG 2 cut(s) 136, 194
MvnI CGCG 3 cut(s) 76, 130, 190
NciI CCSGG 1 cut(s) 179
NcoI CCATGG 1 cut(s) 1151
NdeI CATATG 2 cut(s) 925, 948
NdeII GATC 6 cut(s) 39, 182, 334, 797, 943, 1128
NheI GCTAGC 1 cut(s) 436
NlaIII CATG 6 cut(s) 289, 892, 989, 1046, 1067, 1155
NlaIV GGNNCC 1 cut(s) 184
NsiI ATGCAT 1 cut(s) 1084
NspI RCATGY 1 cut(s) 1046
NspV TTCGAA 1 cut(s) 856
OliI CACNNNNGTG 1 cut(s) 378
PciI ACATGT 1 cut(s) 1042
PciSI GCTCTTC 1 cut(s) 757
PcsI WCGNNNNNNNCGW 1 cut(s) 267
PdmI GAANNNNTTC 2 cut(s) 555, 766
PfeI GAWTC 2 cut(s) 156, 973
PflMI CCANNNNNTGG 1 cut(s) 704
PleI GAGTC 1 cut(s) 863
PpsI GAGTC 1 cut(s) 863
Ppu21I YACGTR 1 cut(s) 902
PscI ACATGT 1 cut(s) 1042
PsiI TTATAA 1 cut(s) 683
Psp6I CCWGG 2 cut(s) 134, 192
PspGI CCWGG 2 cut(s) 134, 192
PspN4I GGNNCC 1 cut(s) 184
PspPI GGNCC 1 cut(s) 132
PstI CTGCAG 1 cut(s) 334
PstNI CAGNNNCTG 2 cut(s) 199, 1094
PsuI RGATCY 2 cut(s) 182, 797
PvuII CAGCTG 1 cut(s) 1091
RsaI GTAC 2 cut(s) 652, 1041
RsaNI GTAC 2 cut(s) 651, 1040
RseI CAYNNNNRTG 2 cut(s) 378, 1077
SapI GCTCTTC 1 cut(s) 757
SaqAI TTAA 2 cut(s) 864, 909
Sau3AI GATC 6 cut(s) 39, 182, 334, 797, 943, 1128
Sau96I GGNCC 1 cut(s) 132
SchI GAGTC 1 cut(s) 863
ScrFI CCNGG 3 cut(s) 136, 179, 194
SduI GDGCHC 1 cut(s) 375
SfaNI GCATC 4 cut(s) 41, 229, 817, 1091
SfcI CTRYAG 1 cut(s) 330
SfuI TTCGAA 1 cut(s) 856
SmiMI CAYNNNNRTG 2 cut(s) 378, 1077
SmlI CTYRAG 2 cut(s) 709, 771
SmoI CTYRAG 2 cut(s) 709, 771
SspMI CTAG 3 cut(s) 89, 396, 437
StyD4I CCNGG 3 cut(s) 134, 177, 192
StyI CCWWGG 4 cut(s) 88, 113, 646, 1151
TaaI ACNGT 1 cut(s) 688
TaiI ACGT 3 cut(s) 904, 982, 1162
TaqI TCGA 6 cut(s) 207, 237, 856, 971, 976, 1110
TatI WGTACW 1 cut(s) 1039
TauI GCSGC 7 cut(s) 72, 133, 225, 245, 248, 251, 254
TfiI GAWTC 2 cut(s) 156, 973
Tru1I TTAA 2 cut(s) 864, 909
Tru9I TTAA 2 cut(s) 864, 909
TscAI CASTG 2 cut(s) 313, 385
TseI GCWGC 5 cut(s) 8, 171, 329, 776, 1091
TspDTI ATGAA 6 cut(s) 689, 877, 957, 1002, 1035, 1061
TspRI CASTG 2 cut(s) 313, 385
Van91I CCANNNNNTGG 1 cut(s) 704
VneI GTGCAC 1 cut(s) 371
XagI CCTNNNNNAGG 1 cut(s) 645
XapI RAATTY 5 cut(s) 486, 676, 884, 937, 1237
XbaI TCTAGA 1 cut(s) 395
XceI RCATGY 1 cut(s) 1046
XmaJI CCTAGG 1 cut(s) 88
XmnI GAANNNNTTC 2 cut(s) 555, 766
XspI CTAG 3 cut(s) 89, 396, 437
ZraI GACGTC 1 cut(s) 980
Zsp2I ATGCAT 1 cut(s) 1084
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.