FvH4_4g08890

Putative serine esterase (DUF676)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0007465
Physical Location & Seq
Forward (+)
9788132 .. 9795071
6940 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g08890.t7

Sequence Viewer

Length: 867 bp
ATGGGTGAGAGGCTAGCTGAGGAGGTATTAGCTGTTGTCAGAAAAAGGTTGGAGGTGCGGAAAATTTCTTTTGTCGCTCACTCTTTAGGGGGGTTGGTTGCAAGGTACGCTATCGGGAGGCTGTACGAACCTTTTCCAAAGCCCGAAGCCACAGGTGATAATGGTAATTGCTTAATTGAAGGGAATACAAATAATTTGACACAATCCCATGATCAACCTCACCAAGGTACGATTGCTGGATTACAAGCCATGAATTTTATAACTGTTGCAACACCACATCTTGGCTCAAGGGGCAATAAACAGCTACCATTTCTCTGTGGTCTTCCCTTTCTTGAAAGAAGGGCTTCTCAAGCTGCCCACTTTATTGTGGGGAGATCTGGGAAGCATCTCTTCTTAACGGATAAAGACGATGGTAGACCTCCCCTTCTTCTTCGAATGGTTAATGACTCTGATGACCTGAAATTCATATCAGCATTACATGCATTTAAGCGTCGTGTGGCATATGCTAACGCAAATTTTGATCATATGGTTGGGTGGAGAACATCATCAATTCGACGCCAACATGAACTTCCAAAGTCCAATCTACTTGAAATAGATGAGAAATATCCACATATTGTACATGTGAAGCAAGAAACTTTTGATGATGTTCATAATAATGCATCTTCAGCTGCCGAAGACCAAAATATCGACTTGGAAGAGAAGATGATCAGAGGTCTCACTCAAATACCATGGGAACGTGTAGATGTCAGTTTTCAAAAGAGCCGACAAAGATATATTGCGCATAATACCATTCAGGTCAAAAGGTATTGGTTGAATTCTGATGGTGCTGATGTGGTATTCCATATCATAGATTACTTCCTTCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF676

Protein Analysis

289

Amino Acids

32.77

Weight (kDa)

8.48

Isoelectric Point (pI)

45.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF676 PF05057 1 - 181 2.4e-48 Putative serine esterase (DUF676)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 260
Acc16I TGCGCA 1 cut(s) 780
AccB7I CCANNNNNTGG 1 cut(s) 281
AccI GTMKAC 1 cut(s) 415
AciI CCGC 1 cut(s) 58
AcsI RAATTY 5 cut(s) 63, 253, 461, 514, 814
AcuI CTGAAG 1 cut(s) 648
AcyI GRCGYC 1 cut(s) 556
AfaI GTAC 4 cut(s) 107, 125, 229, 618
AfiI CCNNNNNNNGG 2 cut(s) 224, 281
AflIII ACRYGT 2 cut(s) 619, 736
AgsI TTSAA 5 cut(s) 179, 335, 590, 755, 814
AjuI GAANNNNNNNTTGG 2 cut(s) 552, 584
AluBI AGCT 5 cut(s) 17, 32, 304, 353, 668
AluI AGCT 5 cut(s) 17, 32, 304, 353, 668
Alw26I GTCTC 1 cut(s) 719
ApeKI GCWGC 2 cut(s) 353, 668
ApoI RAATTY 5 cut(s) 63, 253, 461, 514, 814
Asp700I GAANNNNTTC 2 cut(s) 132, 343
AspLEI GCGC 1 cut(s) 781
AsuHPI GGTGA 3 cut(s) 17, 167, 212
AsuII TTCGAA 1 cut(s) 433
AsuNHI GCTAGC 1 cut(s) 13
BbsI GAAGAC 2 cut(s) 314, 681
BbvCI CCTCAGC 1 cut(s) 18
BbvI GCAGC 2 cut(s) 340, 655
BccI CCATC 2 cut(s) 404, 815
BclI TGATCA 3 cut(s) 211, 520, 705
BcoDI GTCTC 1 cut(s) 719
BfaI CTAG 1 cut(s) 14
BglII AGATCT 1 cut(s) 374
BisI GCNGC 2 cut(s) 354, 669
BlsI GCNGC 2 cut(s) 355, 670
BmsI GCATC 2 cut(s) 394, 668
BmtI GCTAGC 1 cut(s) 17
BpiI GAAGAC 2 cut(s) 314, 681
Bpu10I CCTNAGC 1 cut(s) 18
Bpu14I TTCGAA 1 cut(s) 433
BpuEI CTTGAG 2 cut(s) 271, 333
BsaHI GRCGYC 1 cut(s) 556
BsaI GGTCTC 1 cut(s) 719
BsaJI CCNNGG 2 cut(s) 223, 728
Bsc4I CCNNNNNNNGG 2 cut(s) 224, 281
BseDI CCNNGG 2 cut(s) 223, 728
BseLI CCNNNNNNNGG 2 cut(s) 224, 281
BseMII CTCAG 1 cut(s) 9
BseRI GAGGAG 1 cut(s) 35
BseXI GCAGC 2 cut(s) 340, 655
BslI CCNNNNNNNGG 2 cut(s) 224, 281
BsmAI GTCTC 1 cut(s) 719
Bso31I GGTCTC 1 cut(s) 719
Bsp119I TTCGAA 1 cut(s) 433
Bsp1407I TGTACA 1 cut(s) 616
Bsp143I GATC 4 cut(s) 211, 374, 520, 705
Bsp19I CCATGG 1 cut(s) 728
BspACI CCGC 1 cut(s) 58
BspCNI CTCAG 1 cut(s) 10
BspOI GCTAGC 1 cut(s) 17
BspT104I TTCGAA 1 cut(s) 433
BspTNI GGTCTC 1 cut(s) 719
BsrGI TGTACA 1 cut(s) 616
BssECI CCNNGG 2 cut(s) 223, 728
BssMI GATC 4 cut(s) 211, 374, 520, 705
BssNI GRCGYC 1 cut(s) 556
BssT1I CCWWGG 2 cut(s) 223, 728
Bst4CI ACNGT 1 cut(s) 265
Bst6I CTCTTC 2 cut(s) 395, 690
BstACI GRCGYC 1 cut(s) 556
BstAPI GCANNNNNTGC 1 cut(s) 479
BstAUI TGTACA 1 cut(s) 616
BstBI TTCGAA 1 cut(s) 433
BstC8I GCNNGC 1 cut(s) 15
BstDEI CTNAG 1 cut(s) 18
BstDSI CCRYGG 1 cut(s) 728
BstENI CCTNNNNNAGG 1 cut(s) 222
BstHHI GCGC 1 cut(s) 781
BstKTI GATC 4 cut(s) 214, 377, 523, 708
BstMAI GTCTC 1 cut(s) 719
BstMBI GATC 4 cut(s) 211, 374, 520, 705
BstMWI GCNNNNNNNGC 5 cut(s) 107, 291, 350, 479, 665
BstNSI RCATGY 2 cut(s) 482, 623
BstV1I GCAGC 2 cut(s) 340, 655
BstV2I GAAGAC 2 cut(s) 314, 681
BstX2I RGATCY 1 cut(s) 374
BstYI RGATCY 1 cut(s) 374
BtgI CCRYGG 1 cut(s) 728
Cac8I GCNNGC 1 cut(s) 15
CfoI GCGC 1 cut(s) 781
CseI GACGC 2 cut(s) 479, 564
Csp6I GTAC 4 cut(s) 106, 124, 228, 617
CviAII CATG 6 cut(s) 209, 250, 479, 563, 620, 729
CviQI GTAC 4 cut(s) 106, 124, 228, 617
DdeI CTNAG 1 cut(s) 18
DpnI GATC 4 cut(s) 213, 376, 522, 707
DpnII GATC 4 cut(s) 211, 374, 520, 705
Eam1104I CTCTTC 2 cut(s) 395, 690
EarI CTCTTC 2 cut(s) 395, 690
Eco130I CCWWGG 2 cut(s) 223, 728
Eco31I GGTCTC 1 cut(s) 719
Eco57I CTGAAG 1 cut(s) 648
EcoNI CCTNNNNNAGG 1 cut(s) 222
EcoRI GAATTC 1 cut(s) 814
EcoT14I CCWWGG 2 cut(s) 223, 728
EcoT22I ATGCAT 2 cut(s) 484, 661
ErhI CCWWGG 2 cut(s) 223, 728
FaeI CATG 6 cut(s) 212, 253, 482, 566, 623, 732
FalI AAGNNNNNCTT 4 cut(s) 328, 360, 374, 406
FatI CATG 6 cut(s) 208, 249, 478, 562, 619, 728
FauNDI CATATG 2 cut(s) 502, 525
FbaI TGATCA 3 cut(s) 211, 520, 705
FblI GTMKAC 1 cut(s) 415
Fnu4HI GCNGC 2 cut(s) 354, 669
Fsp4HI GCNGC 2 cut(s) 354, 669
FspBI CTAG 1 cut(s) 14
FspI TGCGCA 1 cut(s) 780
GlaI GCGC 1 cut(s) 780
GluI GCNGC 2 cut(s) 354, 669
HgaI GACGC 2 cut(s) 479, 564
HhaI GCGC 1 cut(s) 781
Hin1I GRCGYC 1 cut(s) 556
Hin1II CATG 6 cut(s) 212, 253, 482, 566, 623, 732
Hin6I GCGC 1 cut(s) 779
HinP1I GCGC 1 cut(s) 779
HinfI GANTC 1 cut(s) 446
HphI GGTGA 3 cut(s) 17, 167, 212
Hpy166II GTNNAC 1 cut(s) 416
Hpy188I TCNGA 4 cut(s) 41, 451, 710, 820
Hpy188III TCNNGA 2 cut(s) 115, 332
Hpy8I GTNNAC 1 cut(s) 416
Hpy99I CGWCG 2 cut(s) 495, 558
HpyAV CCTTC 3 cut(s) 173, 333, 434
HpyCH4III ACNGT 1 cut(s) 265
HpyCH4IV ACGT 1 cut(s) 736
HpyCH4V TGCA 4 cut(s) 101, 269, 482, 659
HpyF10VI GCNNNNNNNGC 5 cut(s) 107, 291, 350, 479, 665
HpyF3I CTNAG 1 cut(s) 18
HpySE526I ACGT 1 cut(s) 736
Hsp92I GRCGYC 1 cut(s) 556
Hsp92II CATG 6 cut(s) 212, 253, 482, 566, 623, 732
HspAI GCGC 1 cut(s) 779
Ksp22I TGATCA 3 cut(s) 211, 520, 705
Kzo9I GATC 4 cut(s) 211, 374, 520, 705
LpnPI CCDG 5 cut(s) 138, 222, 363, 470, 779
Lsp1109I GCAGC 2 cut(s) 340, 655
LweI GCATC 2 cut(s) 394, 668
MaeI CTAG 1 cut(s) 14
MaeII ACGT 1 cut(s) 736
MalI GATC 4 cut(s) 213, 376, 522, 707
MboI GATC 4 cut(s) 211, 374, 520, 705
MboII GAAGA 8 cut(s) 314, 382, 419, 422, 654, 686, 707, 712
MflI RGATCY 1 cut(s) 374
MluCI AATT 9 cut(s) 63, 166, 174, 193, 253, 461, 514, 549, 814
MlyI GAGTC 1 cut(s) 440
MmeI TCCRAC 1 cut(s) 30
MnlI CCTC 8 cut(s) 3, 13, 16, 46, 111, 228, 429, 704
Mph1103I ATGCAT 2 cut(s) 484, 661
MroXI GAANNNNTTC 2 cut(s) 132, 343
MseI TTAA 4 cut(s) 173, 395, 441, 486
MslI CAYNNNNRTG 1 cut(s) 654
MspA1I CMGCKG 1 cut(s) 668
MwoI GCNNNNNNNGC 5 cut(s) 107, 291, 350, 479, 665
NcoI CCATGG 1 cut(s) 728
NdeI CATATG 2 cut(s) 502, 525
NdeII GATC 4 cut(s) 211, 374, 520, 705
NheI GCTAGC 1 cut(s) 13
NlaIII CATG 6 cut(s) 212, 253, 482, 566, 623, 732
NsbI TGCGCA 1 cut(s) 780
NsiI ATGCAT 2 cut(s) 484, 661
NspI RCATGY 2 cut(s) 482, 623
NspV TTCGAA 1 cut(s) 433
PciI ACATGT 1 cut(s) 619
PdmI GAANNNNTTC 2 cut(s) 132, 343
PflMI CCANNNNNTGG 1 cut(s) 281
PkrI GCNGC 2 cut(s) 355, 670
PleI GAGTC 1 cut(s) 440
PpsI GAGTC 1 cut(s) 440
PscI ACATGT 1 cut(s) 619
PsiI TTATAA 1 cut(s) 260
PsuI RGATCY 1 cut(s) 374
PvuII CAGCTG 1 cut(s) 668
RsaI GTAC 4 cut(s) 107, 125, 229, 618
RsaNI GTAC 4 cut(s) 106, 124, 228, 617
RseI CAYNNNNRTG 1 cut(s) 654
SaqAI TTAA 4 cut(s) 173, 395, 441, 486
SatI GCNGC 2 cut(s) 354, 669
Sau3AI GATC 4 cut(s) 211, 374, 520, 705
SchI GAGTC 1 cut(s) 440
SfaNI GCATC 2 cut(s) 394, 668
SfuI TTCGAA 1 cut(s) 433
SmiMI CAYNNNNRTG 1 cut(s) 654
SmlI CTYRAG 2 cut(s) 286, 348
SmoI CTYRAG 2 cut(s) 286, 348
Sse9I AATT 9 cut(s) 63, 166, 174, 193, 253, 461, 514, 549, 814
SsiI CCGC 1 cut(s) 58
SspMI CTAG 1 cut(s) 14
StyI CCWWGG 2 cut(s) 223, 728
TaaI ACNGT 1 cut(s) 265
TaiI ACGT 1 cut(s) 739
TaqI TCGA 3 cut(s) 433, 553, 687
TasI AATT 9 cut(s) 63, 166, 174, 193, 253, 461, 514, 549, 814
TatI WGTACW 1 cut(s) 616
Tru1I TTAA 4 cut(s) 173, 395, 441, 486
Tru9I TTAA 4 cut(s) 173, 395, 441, 486
TseI GCWGC 2 cut(s) 353, 668
TspDTI ATGAA 4 cut(s) 266, 454, 579, 638
TspGWI ACGGA 1 cut(s) 413
Van91I CCANNNNNTGG 1 cut(s) 281
XagI CCTNNNNNAGG 1 cut(s) 222
XapI RAATTY 5 cut(s) 63, 253, 461, 514, 814
XceI RCATGY 2 cut(s) 482, 623
XmiI GTMKAC 1 cut(s) 415
XmnI GAANNNNTTC 2 cut(s) 132, 343
XspI CTAG 1 cut(s) 14
Zsp2I ATGCAT 2 cut(s) 484, 661
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.