Rh4CG127400

Putative serine esterase (DUF676)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0007465
Physical Location & Seq
Forward (+)
25019339 .. 25025567
6229 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG127400.1

Sequence Viewer

Length: 1284 bp
ATGATTGCAGCCACTTCTCCTGTCAACTCCGCATCCCCGATCCCCGCCCCGAACCCGACCCGAACCCGAGGCCCGCGCCACCTCCGCCTGGGGTTTCCTTCCTTCTCCGAGCCAAGGCTCTGTGCGACGCGGCCCAGGATGGACTTCCTGCACAGAATCGTAGGCGGAGGCTGCTTCCGGGGATCCACGCGCCAGGGGCTGAAGGTCGAGATTGATAGCGGCGGCGAGGATATCTTCGCCGCCGCCGCCAACAACGCCGTTCCCGAACATCTCGTCGTCATGGTCAATGGAATTATCGGCAGTGCTTCAGATTGGAGATATGCTGCAGATCAGTTTGTGAAAAAGCTTCCTGATAAAGTAATTGTGCACCGCAGTGAATGTAACTCTTCTAGATTGACATTTGATGGAGTTGACTTGATGGGTGAGAGGCTAGCTAAGGAGGTATTAGATGTTGTCAGAAAAAGGTCAGAGGTGCAGAAAATTTCCTTTGTCGCTCACTCTTTGGGGGGGTTGGTTGCAAGGTATGCTATCGGGATGCTTTACGAACCTTTTCCAAAACCCGAACCCTCAGGTGATAATGGTAATTGCATAATTGAAGGGAATATAAATAATTTGACACAATCCCTTGACCAGCCTCACCAAGGTACGATTGCTGGATTACAAGCTATGAATTTTATAACAGTTGCAACACCACATCTTGGCTCAAGGGGAAATAAACAGCTCCCATTTCTCTGTGGTCTTCCCTTTCTTGAAAGAAGAGCTTCTCAAGCTGCCCACTTTATTGTGGGGAGATCTGGGAAGCATCTCTTCCTAACAGATAAAGACGATGGCAAACCTCCCCTTCTTCTTCGAATGGTTAACGACTCTGATGACCTGAAATTCATGTCAGCATTACGTGCATTTAAGCGTCGTGTGGCATATGCTAATGCAAATTTTGATCATATGGTTGGGTGGAGAACTTCATCAATTCGACGTCAACATGAACTTCCGAAGTCCAATCTACTTGTAACAGATGAAAAATATCCACATATTGTACATGTGAAGCAAGAAACTTTAGATGATATTCATAATAATGCATCTTCAGCTGTTGAAAACCAAAATATCGACTTGGAAGAGGAGATGATCAGAGGTCTCACTCAAATACCATGGGAACGTGTAGATGTCAGCTTTCAAAAGAGCCGACAAAGATACATTGCTCATAATACCATTCAGGTCAAAAGATATTGGTTGAATTCTGATGGTGCTGATGTGGTTTTCCATATCATAGATTACTTCCTTCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF676

Protein Analysis

427

Amino Acids

47.73

Weight (kDa)

9.0

Isoelectric Point (pI)

46.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF676 PF05057 88 - 320 6.6e-69 Putative serine esterase (DUF676)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 677
AatII GACGTC 1 cut(s) 976
AccB7I CCANNNNNTGG 1 cut(s) 698
AccII CGCG 3 cut(s) 76, 130, 190
AclWI GGATC 3 cut(s) 34, 177, 190
AcsI RAATTY 5 cut(s) 480, 670, 878, 931, 1231
AcuI CTGAAG 3 cut(s) 221, 291, 1065
AcyI GRCGYC 1 cut(s) 973
AfaI GTAC 2 cut(s) 646, 1035
AfiI CCNNNNNNNGG 4 cut(s) 88, 114, 641, 698
AflIII ACRYGT 2 cut(s) 1036, 1153
AgsI TTSAA 5 cut(s) 596, 752, 1091, 1172, 1231
AjnI CCWGG 3 cut(s) 87, 134, 192
AleI CACNNNNGTG 1 cut(s) 372
AluBI AGCT 8 cut(s) 346, 434, 665, 721, 761, 770, 1085, 1167
AluI AGCT 8 cut(s) 346, 434, 665, 721, 761, 770, 1085, 1167
Alw21I GWGCWC 1 cut(s) 369
Alw26I GTCTC 1 cut(s) 1136
Alw44I GTGCAC 1 cut(s) 365
AlwI GGATC 3 cut(s) 34, 177, 190
AlwNI CAGNNNCTG 1 cut(s) 199
Ama87I CYCGRG 1 cut(s) 66
AoxI GGCC 2 cut(s) 70, 131
ApaLI GTGCAC 1 cut(s) 365
ApeKI GCWGC 4 cut(s) 8, 171, 323, 770
ApoI RAATTY 5 cut(s) 480, 670, 878, 931, 1231
ArsI GACNNNNNNTTYG 2 cut(s) 258, 290
Asp700I GAANNNNTTC 2 cut(s) 549, 760
AspLEI GCGC 2 cut(s) 78, 192
AspS9I GGNCC 2 cut(s) 71, 132
AsuC2I CCSGG 1 cut(s) 179
AsuHPI GGTGA 3 cut(s) 434, 584, 629
AsuII TTCGAA 1 cut(s) 850
AsuNHI GCTAGC 1 cut(s) 430
AvaI CYCGRG 1 cut(s) 66
AxyI CCTNAGG 1 cut(s) 568
BaeGI GKGCMC 1 cut(s) 369
BamHI GGATCC 1 cut(s) 182
BbsI GAAGAC 1 cut(s) 731
Bbv12I GWGCWC 1 cut(s) 369
BbvI GCAGC 4 cut(s) 20, 158, 310, 757
BccI CCATC 5 cut(s) 133, 398, 412, 821, 1232
BceAI ACGGC 1 cut(s) 242
BciT130I CCWGG 3 cut(s) 89, 136, 194
BclI TGATCA 2 cut(s) 937, 1122
BcnI CCSGG 1 cut(s) 179
BcoDI GTCTC 1 cut(s) 1136
BfaI CTAG 2 cut(s) 390, 431
BfmI CTRYAG 1 cut(s) 324
BglII AGATCT 1 cut(s) 791
Bme1390I CCNGG 4 cut(s) 89, 136, 179, 194
BmeT110I CYCGRG 1 cut(s) 66
BmgT120I GGNCC 2 cut(s) 71, 132
BmiI GGNNCC 1 cut(s) 184
BmrFI CCNGG 4 cut(s) 89, 136, 179, 194
BmsI GCATC 4 cut(s) 41, 525, 811, 1085
BmtI GCTAGC 1 cut(s) 434
BpiI GAAGAC 1 cut(s) 731
Bpu10I CCTNAGC 1 cut(s) 435
Bpu14I TTCGAA 1 cut(s) 850
BpuEI CTTGAG 2 cut(s) 688, 750
BpuMI CCSGG 1 cut(s) 179
BsaAI YACGTR 1 cut(s) 896
BsaHI GRCGYC 1 cut(s) 973
BsaI GGTCTC 1 cut(s) 1136
BsaJI CCNNGG 8 cut(s) 67, 88, 113, 134, 178, 193, 640, 1145
Bsc4I CCNNNNNNNGG 4 cut(s) 88, 114, 641, 698
Bse21I CCTNAGG 1 cut(s) 568
Bse3DI GCAATG 1 cut(s) 1191
BseBI CCWGG 3 cut(s) 89, 136, 194
BseDI CCNNGG 8 cut(s) 67, 88, 113, 134, 178, 193, 640, 1145
BseGI GGATG 3 cut(s) 32, 144, 540
BseLI CCNNNNNNNGG 4 cut(s) 88, 114, 641, 698
BseMI GCAATG 1 cut(s) 1191
BseMII CTCAG 1 cut(s) 582
BseRI GAGGAG 1 cut(s) 1130
BseSI GKGCMC 1 cut(s) 369
BseXI GCAGC 4 cut(s) 20, 158, 310, 757
BsgI GTGCAG 2 cut(s) 134, 494
Bsh1236I CGCG 3 cut(s) 76, 130, 190
BshFI GGCC 2 cut(s) 72, 133
BsiHKAI GWGCWC 1 cut(s) 369
BsiHKCI CYCGRG 1 cut(s) 66
BsiSI CCGG 1 cut(s) 178
BslI CCNNNNNNNGG 4 cut(s) 88, 114, 641, 698
BsmAI GTCTC 1 cut(s) 1136
BsnI GGCC 2 cut(s) 72, 133
Bso31I GGTCTC 1 cut(s) 1136
BsoBI CYCGRG 1 cut(s) 66
Bsp119I TTCGAA 1 cut(s) 850
Bsp1286I GDGCHC 1 cut(s) 369
Bsp1407I TGTACA 1 cut(s) 1033
Bsp143I GATC 6 cut(s) 39, 182, 328, 791, 937, 1122
Bsp19I CCATGG 1 cut(s) 1145
BspANI GGCC 2 cut(s) 72, 133
BspCNI CTCAG 1 cut(s) 581
BspFNI CGCG 3 cut(s) 76, 130, 190
BspLI GGNNCC 1 cut(s) 184
BspMAI CTGCAG 1 cut(s) 328
BspOI GCTAGC 1 cut(s) 434
BspPI GGATC 3 cut(s) 34, 177, 190
BspQI GCTCTTC 1 cut(s) 751
BspT104I TTCGAA 1 cut(s) 850
BspTNI GGTCTC 1 cut(s) 1136
BsrDI GCAATG 1 cut(s) 1191
BsrGI TGTACA 1 cut(s) 1033
BssECI CCNNGG 8 cut(s) 67, 88, 113, 134, 178, 193, 640, 1145
BssMI GATC 6 cut(s) 39, 182, 328, 791, 937, 1122
BssNI GRCGYC 1 cut(s) 973
BssT1I CCWWGG 3 cut(s) 113, 640, 1145
Bst2UI CCWGG 3 cut(s) 89, 136, 194
Bst4CI ACNGT 1 cut(s) 682
Bst6I CTCTTC 4 cut(s) 391, 751, 812, 1107
BstACI GRCGYC 1 cut(s) 973
BstAPI GCANNNNNTGC 2 cut(s) 524, 896
BstAUI TGTACA 1 cut(s) 1033
BstBAI YACGTR 1 cut(s) 896
BstBI TTCGAA 1 cut(s) 850
BstC8I GCNNGC 2 cut(s) 74, 432
BstDEI CTNAG 2 cut(s) 435, 568
BstDSI CCRYGG 1 cut(s) 1145
BstENI CCTNNNNNAGG 1 cut(s) 639
BstF5I GGATG 3 cut(s) 32, 144, 540
BstFNI CGCG 3 cut(s) 76, 130, 190
BstHHI GCGC 2 cut(s) 78, 192
BstKTI GATC 6 cut(s) 42, 185, 331, 794, 940, 1125
BstMAI GTCTC 1 cut(s) 1136
BstMBI GATC 6 cut(s) 39, 182, 328, 791, 937, 1122
BstMWI GCNNNNNNNGC 9 cut(s) 84, 171, 196, 245, 254, 524, 767, 896, 1082
BstNI CCWGG 3 cut(s) 89, 136, 194
BstNSI RCATGY 1 cut(s) 1040
BstSCI CCNGG 4 cut(s) 87, 134, 177, 192
BstSFI CTRYAG 1 cut(s) 324
BstSLI GKGCMC 1 cut(s) 369
BstUI CGCG 3 cut(s) 76, 130, 190
BstV1I GCAGC 4 cut(s) 20, 158, 310, 757
BstV2I GAAGAC 1 cut(s) 731
BstX2I RGATCY 2 cut(s) 182, 791
BstYI RGATCY 2 cut(s) 182, 791
Bsu36I CCTNAGG 1 cut(s) 568
BsuRI GGCC 2 cut(s) 72, 133
BtgI CCRYGG 1 cut(s) 1145
BtsCI GGATG 3 cut(s) 32, 144, 540
BtsI GCAGTG 2 cut(s) 307, 379
BtsIMutI CAGTG 2 cut(s) 307, 379
Cac8I GCNNGC 2 cut(s) 74, 432
CaiI CAGNNNCTG 1 cut(s) 199
CfoI GCGC 2 cut(s) 78, 192
Cfr13I GGNCC 2 cut(s) 71, 132
CseI GACGC 2 cut(s) 136, 896
Csp6I GTAC 2 cut(s) 645, 1034
CviAII CATG 5 cut(s) 280, 883, 980, 1037, 1146
CviQI GTAC 2 cut(s) 645, 1034
DdeI CTNAG 2 cut(s) 435, 568
DpnI GATC 6 cut(s) 41, 184, 330, 793, 939, 1124
DpnII GATC 6 cut(s) 39, 182, 328, 791, 937, 1122
Eam1104I CTCTTC 4 cut(s) 391, 751, 812, 1107
EarI CTCTTC 4 cut(s) 391, 751, 812, 1107
EciI GGCGGA 2 cut(s) 74, 180
Eco130I CCWWGG 3 cut(s) 113, 640, 1145
Eco31I GGTCTC 1 cut(s) 1136
Eco32I GATATC 1 cut(s) 232
Eco57I CTGAAG 3 cut(s) 221, 291, 1065
Eco81I CCTNAGG 1 cut(s) 568
Eco88I CYCGRG 1 cut(s) 66
EcoNI CCTNNNNNAGG 1 cut(s) 639
EcoRI GAATTC 1 cut(s) 1231
EcoRII CCWGG 3 cut(s) 87, 134, 192
EcoRV GATATC 1 cut(s) 232
EcoT14I CCWWGG 3 cut(s) 113, 640, 1145
EcoT22I ATGCAT 1 cut(s) 1078
ErhI CCWWGG 3 cut(s) 113, 640, 1145
FaeI CATG 5 cut(s) 283, 886, 983, 1040, 1149
FalI AAGNNNNNCTT 4 cut(s) 745, 777, 791, 823
FatI CATG 5 cut(s) 279, 882, 979, 1036, 1145
FauI CCCGC 2 cut(s) 52, 81
FauNDI CATATG 2 cut(s) 919, 942
FbaI TGATCA 2 cut(s) 937, 1122
FokI GGATG 3 cut(s) 19, 151, 547
FspBI CTAG 2 cut(s) 390, 431
GlaI GCGC 2 cut(s) 77, 191
HaeIII GGCC 2 cut(s) 72, 133
HapII CCGG 1 cut(s) 178
HgaI GACGC 2 cut(s) 136, 896
HhaI GCGC 2 cut(s) 78, 192
Hin1I GRCGYC 1 cut(s) 973
Hin1II CATG 5 cut(s) 283, 886, 983, 1040, 1149
Hin6I GCGC 2 cut(s) 76, 190
HinP1I GCGC 2 cut(s) 76, 190
HincII GTYRAC 4 cut(s) 25, 412, 859, 977
HindII GTYRAC 4 cut(s) 25, 412, 859, 977
HindIII AAGCTT 1 cut(s) 344
HinfI GANTC 2 cut(s) 156, 863
HpaI GTTAAC 1 cut(s) 859
HpaII CCGG 1 cut(s) 178
HphI GGTGA 3 cut(s) 434, 584, 629
Hpy166II GTNNAC 5 cut(s) 25, 367, 412, 859, 977
Hpy188I TCNGA 8 cut(s) 109, 310, 458, 469, 868, 990, 1127, 1237
Hpy188III TCNNGA 6 cut(s) 208, 263, 350, 390, 532, 749
Hpy8I GTNNAC 5 cut(s) 25, 367, 412, 859, 977
Hpy99I CGWCG 4 cut(s) 130, 278, 912, 975
HpyAV CCTTC 5 cut(s) 108, 112, 196, 590, 851
HpyCH4III ACNGT 1 cut(s) 682
HpyCH4IV ACGT 3 cut(s) 895, 973, 1153
HpyF10VI GCNNNNNNNGC 9 cut(s) 84, 171, 196, 245, 254, 524, 767, 896, 1082
HpyF3I CTNAG 2 cut(s) 435, 568
HpySE526I ACGT 3 cut(s) 895, 973, 1153
Hsp92I GRCGYC 1 cut(s) 973
Hsp92II CATG 5 cut(s) 283, 886, 983, 1040, 1149
HspAI GCGC 2 cut(s) 76, 190
Ksp22I TGATCA 2 cut(s) 937, 1122
KspAI GTTAAC 1 cut(s) 859
Kzo9I GATC 6 cut(s) 39, 182, 328, 791, 937, 1122
LguI GCTCTTC 1 cut(s) 751
LmnI GCTCC 1 cut(s) 726
Lsp1109I GCAGC 4 cut(s) 20, 158, 310, 757
LweI GCATC 4 cut(s) 41, 525, 811, 1085
MaeI CTAG 2 cut(s) 390, 431
MaeII ACGT 3 cut(s) 895, 973, 1153
MaeIII GTNAC 2 cut(s) 380, 1006
MalI GATC 6 cut(s) 41, 184, 330, 793, 939, 1124
MboI GATC 6 cut(s) 39, 182, 328, 791, 937, 1122
MboII GAAGA 9 cut(s) 226, 378, 731, 768, 799, 836, 839, 1071, 1124
MflI RGATCY 2 cut(s) 182, 791
MhlI GDGCHC 1 cut(s) 369
MlyI GAGTC 1 cut(s) 857
Mph1103I ATGCAT 1 cut(s) 1078
MroXI GAANNNNTTC 2 cut(s) 549, 760
MseI TTAA 2 cut(s) 858, 903
MslI CAYNNNNRTG 2 cut(s) 372, 1071
MspA1I CMGCKG 1 cut(s) 1085
MspI CCGG 1 cut(s) 178
MspR9I CCNGG 4 cut(s) 89, 136, 179, 194
MvaI CCWGG 3 cut(s) 89, 136, 194
MvnI CGCG 3 cut(s) 76, 130, 190
MwoI GCNNNNNNNGC 9 cut(s) 84, 171, 196, 245, 254, 524, 767, 896, 1082
NciI CCSGG 1 cut(s) 179
NcoI CCATGG 1 cut(s) 1145
NdeI CATATG 2 cut(s) 919, 942
NdeII GATC 6 cut(s) 39, 182, 328, 791, 937, 1122
NheI GCTAGC 1 cut(s) 430
NlaIII CATG 5 cut(s) 283, 886, 983, 1040, 1149
NlaIV GGNNCC 1 cut(s) 184
NsiI ATGCAT 1 cut(s) 1078
NspI RCATGY 1 cut(s) 1040
NspV TTCGAA 1 cut(s) 850
OliI CACNNNNGTG 1 cut(s) 372
PciI ACATGT 1 cut(s) 1036
PciSI GCTCTTC 1 cut(s) 751
PcsI WCGNNNNNNNCGW 1 cut(s) 261
PdmI GAANNNNTTC 2 cut(s) 549, 760
PfeI GAWTC 1 cut(s) 156
PflMI CCANNNNNTGG 1 cut(s) 698
PleI GAGTC 1 cut(s) 857
PpsI GAGTC 1 cut(s) 857
Ppu21I YACGTR 1 cut(s) 896
PscI ACATGT 1 cut(s) 1036
PsiI TTATAA 1 cut(s) 677
Psp6I CCWGG 3 cut(s) 87, 134, 192
PspGI CCWGG 3 cut(s) 87, 134, 192
PspN4I GGNNCC 1 cut(s) 184
PspPI GGNCC 2 cut(s) 71, 132
PstI CTGCAG 1 cut(s) 328
PstNI CAGNNNCTG 1 cut(s) 199
PsuI RGATCY 2 cut(s) 182, 791
PvuII CAGCTG 1 cut(s) 1085
RsaI GTAC 2 cut(s) 646, 1035
RsaNI GTAC 2 cut(s) 645, 1034
RseI CAYNNNNRTG 2 cut(s) 372, 1071
SapI GCTCTTC 1 cut(s) 751
SaqAI TTAA 2 cut(s) 858, 903
Sau3AI GATC 6 cut(s) 39, 182, 328, 791, 937, 1122
Sau96I GGNCC 2 cut(s) 71, 132
SchI GAGTC 1 cut(s) 857
ScrFI CCNGG 4 cut(s) 89, 136, 179, 194
SduI GDGCHC 1 cut(s) 369
SfaNI GCATC 4 cut(s) 41, 525, 811, 1085
SfcI CTRYAG 1 cut(s) 324
SfuI TTCGAA 1 cut(s) 850
SmiMI CAYNNNNRTG 2 cut(s) 372, 1071
SmlI CTYRAG 2 cut(s) 703, 765
SmoI CTYRAG 2 cut(s) 703, 765
SspMI CTAG 2 cut(s) 390, 431
StyD4I CCNGG 4 cut(s) 87, 134, 177, 192
StyI CCWWGG 3 cut(s) 113, 640, 1145
TaaI ACNGT 1 cut(s) 682
TaiI ACGT 3 cut(s) 898, 976, 1156
TaqI TCGA 4 cut(s) 207, 850, 970, 1104
TatI WGTACW 1 cut(s) 1033
TauI GCSGC 6 cut(s) 133, 222, 225, 242, 245, 248
TfiI GAWTC 1 cut(s) 156
Tru1I TTAA 2 cut(s) 858, 903
Tru9I TTAA 2 cut(s) 858, 903
TscAI CASTG 2 cut(s) 307, 379
TseI GCWGC 4 cut(s) 8, 171, 323, 770
TspDTI ATGAA 6 cut(s) 683, 871, 951, 996, 1029, 1055
TspRI CASTG 2 cut(s) 307, 379
Van91I CCANNNNNTGG 1 cut(s) 698
VneI GTGCAC 1 cut(s) 365
XagI CCTNNNNNAGG 1 cut(s) 639
XapI RAATTY 5 cut(s) 480, 670, 878, 931, 1231
XbaI TCTAGA 1 cut(s) 389
XceI RCATGY 1 cut(s) 1040
XmnI GAANNNNTTC 2 cut(s) 549, 760
XspI CTAG 2 cut(s) 390, 431
ZraI GACGTC 1 cut(s) 974
Zsp2I ATGCAT 1 cut(s) 1078
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.