MD16G1266100.v1.1

Putative serine esterase (DUF676)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0007465
Physical Location & Seq
Reverse (-)
33913801 .. 33916189
2389 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1266100.v1.1.491

Sequence Viewer

Length: 849 bp
ATGCAGGTATTAGCTGTCATCAGAAAGAGGTCTGAGGTGTGGAAAATATCCTTTGTGGCTCATTCCTTAGGTGGGTTGGTTGCAAGGTATGCCATCGGAAGGCTGTATGCACCTTTTCCAAAATCAGAACCGTCGTGTCAAAATGGTAATTGGGTAATTGATGGGCATACAAATAATTTGACACAATCCATTGACCAACCTCACCATGGTACAATTGCTGGACTGGAAGCAATGAACTTTATAACGGTCGCAACACCACATCTTGGTTCATGGGGAAATAAACAGCTCCCATTTCTCTGTGGTCTTCCTTTTCTCGAGAGAAGAGCTTCTCAAACTGCACACTTTATTGCTGGGAGATCTGGGAAGCATCTCTTCCTAACAGACGATGATGATGGCAGACCTCCTCTTCTTGTTCGAATGGTTAATGACTGTGATGACCTAAAATTCATATCAGCATTACGTGCATTTAAATGTCGTGTGGCATATGCTAATGCAAATTATGATTATATGGTTGGGTGGAGAACATCATCAATCCGGCGTCAATATGAACTTCCAAAGTCCAACCTCCTTGTAACCGATGAGAGATATCCACATATTGTATATATCAAGCGAGAAACTTCTGATCATATTCATATTAAAGCATCGTCAAGTGCTCAAGACTTTAACATAGACTTGGAAGAGGAGATGATTAGAGGTCTTACTCAGATACCTTGGGAACGCGTGGATGTTAGCTTTAAGAAAAGCACACAACGATATATTGCTCATAATACCATTCAGGTGAAGAGTTATTGGTTCAATTCTGATGGTGCGGATGTGGTTTTCCATATGATAGATTGCTTCCTTCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF676

Protein Analysis

283

Amino Acids

32.2

Weight (kDa)

8.54

Isoelectric Point (pI)

40.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF676 PF05057 3 - 175 1.8e-42 Putative serine esterase (DUF676)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 242
AccB7I CCANNNNNTGG 1 cut(s) 263
AccII CGCG 1 cut(s) 720
AciI CCGC 1 cut(s) 809
AcsI RAATTY 1 cut(s) 443
AcyI GRCGYC 1 cut(s) 538
AfaI GTAC 1 cut(s) 211
AfiI CCNNNNNNNGG 4 cut(s) 72, 99, 206, 263
AflIII ACRYGT 1 cut(s) 718
AgsI TTSAA 1 cut(s) 796
AluBI AGCT 4 cut(s) 14, 286, 326, 732
AluI AGCT 4 cut(s) 14, 286, 326, 732
Alw21I GWGCWC 1 cut(s) 655
Ama87I CYCGRG 1 cut(s) 314
ApoI RAATTY 1 cut(s) 443
Asp700I GAANNNNTTC 1 cut(s) 325
AsuHPI GGTGA 2 cut(s) 194, 790
AsuII TTCGAA 1 cut(s) 415
AvaI CYCGRG 1 cut(s) 314
AxyI CCTNAGG 1 cut(s) 67
BbsI GAAGAC 1 cut(s) 296
Bbv12I GWGCWC 1 cut(s) 655
BccI CCATC 4 cut(s) 101, 155, 386, 797
BclI TGATCA 1 cut(s) 622
BglII AGATCT 1 cut(s) 356
BmeT110I CYCGRG 1 cut(s) 314
BmsI GCATC 2 cut(s) 376, 650
BpiI GAAGAC 1 cut(s) 296
Bpu14I TTCGAA 1 cut(s) 415
BpuEI CTTGAG 1 cut(s) 639
BsaAI YACGTR 1 cut(s) 461
BsaHI GRCGYC 1 cut(s) 538
BsaJI CCNNGG 2 cut(s) 205, 710
Bsc4I CCNNNNNNNGG 4 cut(s) 72, 99, 206, 263
Bse1I ACTGG 1 cut(s) 228
Bse21I CCTNAGG 1 cut(s) 67
Bse3DI GCAATG 1 cut(s) 237
BseDI CCNNGG 2 cut(s) 205, 710
BseGI GGATG 2 cut(s) 730, 817
BseLI CCNNNNNNNGG 4 cut(s) 72, 99, 206, 263
BseMI GCAATG 1 cut(s) 237
BseMII CTCAG 2 cut(s) 24, 716
BseNI ACTGG 1 cut(s) 228
BseRI GAGGAG 2 cut(s) 393, 695
BseYI CCCAGC 1 cut(s) 350
BsgI GTGCAG 1 cut(s) 321
Bsh1236I CGCG 1 cut(s) 720
Bsh1285I CGRYCG 1 cut(s) 249
BsiEI CGRYCG 1 cut(s) 249
BsiHKAI GWGCWC 1 cut(s) 655
BsiHKCI CYCGRG 1 cut(s) 314
BsiSI CCGG 1 cut(s) 535
BslI CCNNNNNNNGG 4 cut(s) 72, 99, 206, 263
BsoBI CYCGRG 1 cut(s) 314
Bsp119I TTCGAA 1 cut(s) 415
Bsp1286I GDGCHC 1 cut(s) 655
Bsp143I GATC 2 cut(s) 356, 622
Bsp19I CCATGG 1 cut(s) 205
BspACI CCGC 1 cut(s) 809
BspCNI CTCAG 2 cut(s) 25, 715
BspFNI CGCG 1 cut(s) 720
BspQI GCTCTTC 1 cut(s) 316
BspT104I TTCGAA 1 cut(s) 415
BsrDI GCAATG 1 cut(s) 237
BsrI ACTGG 1 cut(s) 228
BssECI CCNNGG 2 cut(s) 205, 710
BssMI GATC 2 cut(s) 356, 622
BssNI GRCGYC 1 cut(s) 538
BssT1I CCWWGG 2 cut(s) 205, 710
Bst4CI ACNGT 3 cut(s) 132, 247, 431
Bst6I CTCTTC 5 cut(s) 316, 377, 411, 672, 776
BstACI GRCGYC 1 cut(s) 538
BstAPI GCANNNNNTGC 2 cut(s) 89, 461
BstBAI YACGTR 1 cut(s) 461
BstBI TTCGAA 1 cut(s) 415
BstDEI CTNAG 3 cut(s) 33, 67, 702
BstDSI CCRYGG 1 cut(s) 205
BstF5I GGATG 2 cut(s) 730, 817
BstFNI CGCG 1 cut(s) 720
BstKTI GATC 2 cut(s) 359, 625
BstMBI GATC 2 cut(s) 356, 622
BstMCI CGRYCG 1 cut(s) 249
BstMWI GCNNNNNNNGC 2 cut(s) 89, 461
BstUI CGCG 1 cut(s) 720
BstV2I GAAGAC 1 cut(s) 296
BstX2I RGATCY 1 cut(s) 356
BstYI RGATCY 1 cut(s) 356
Bsu36I CCTNAGG 1 cut(s) 67
BtgI CCRYGG 1 cut(s) 205
BtsCI GGATG 2 cut(s) 730, 817
CseI GACGC 1 cut(s) 527
Csp6I GTAC 1 cut(s) 210
CviAII CATG 2 cut(s) 206, 270
CviJI RGCY 6 cut(s) 14, 59, 103, 286, 326, 732
CviKI_1 RGCY 6 cut(s) 14, 59, 103, 286, 326, 732
CviQI GTAC 1 cut(s) 210
DdeI CTNAG 3 cut(s) 33, 67, 702
DpnI GATC 2 cut(s) 358, 624
DpnII GATC 2 cut(s) 356, 622
DraI TTTAAA 1 cut(s) 469
Eam1104I CTCTTC 5 cut(s) 316, 377, 411, 672, 776
EarI CTCTTC 5 cut(s) 316, 377, 411, 672, 776
Eco130I CCWWGG 2 cut(s) 205, 710
Eco32I GATATC 1 cut(s) 587
Eco81I CCTNAGG 1 cut(s) 67
Eco88I CYCGRG 1 cut(s) 314
EcoRV GATATC 1 cut(s) 587
EcoT14I CCWWGG 2 cut(s) 205, 710
ErhI CCWWGG 2 cut(s) 205, 710
FaeI CATG 2 cut(s) 209, 273
FalI AAGNNNNNCTT 2 cut(s) 356, 388
FatI CATG 2 cut(s) 205, 269
FauNDI CATATG 2 cut(s) 484, 825
FbaI TGATCA 1 cut(s) 622
FokI GGATG 2 cut(s) 737, 824
GsaI CCCAGC 1 cut(s) 354
HapII CCGG 1 cut(s) 535
HgaI GACGC 1 cut(s) 527
Hin1I GRCGYC 1 cut(s) 538
Hin1II CATG 2 cut(s) 209, 273
HpaII CCGG 1 cut(s) 535
HphI GGTGA 2 cut(s) 194, 790
Hpy188I TCNGA 7 cut(s) 23, 34, 98, 127, 622, 705, 802
Hpy188III TCNNGA 3 cut(s) 314, 316, 656
Hpy99I CGWCG 1 cut(s) 136
HpyAV CCTTC 1 cut(s) 93
HpyCH4III ACNGT 3 cut(s) 132, 247, 431
HpyCH4IV ACGT 1 cut(s) 460
HpyCH4V TGCA 6 cut(s) 4, 83, 110, 338, 464, 494
HpyF10VI GCNNNNNNNGC 2 cut(s) 89, 461
HpyF3I CTNAG 3 cut(s) 33, 67, 702
HpySE526I ACGT 1 cut(s) 460
Hsp92I GRCGYC 1 cut(s) 538
Hsp92II CATG 2 cut(s) 209, 273
Ksp22I TGATCA 1 cut(s) 622
Kzo9I GATC 2 cut(s) 356, 622
LguI GCTCTTC 1 cut(s) 316
LmnI GCTCC 1 cut(s) 291
LpnPI CCDG 6 cut(s) 204, 209, 336, 345, 548, 761
LweI GCATC 2 cut(s) 376, 650
MaeII ACGT 1 cut(s) 460
MaeIII GTNAC 1 cut(s) 571
MalI GATC 2 cut(s) 358, 624
MboI GATC 2 cut(s) 356, 622
MboII GAAGA 6 cut(s) 296, 333, 364, 398, 689, 793
MfeI CAATTG 1 cut(s) 213
MflI RGATCY 1 cut(s) 356
MhlI GDGCHC 1 cut(s) 655
MluCI AATT 7 cut(s) 148, 156, 175, 213, 443, 496, 796
MluI ACGCGT 1 cut(s) 718
MmeI TCCRAC 1 cut(s) 585
MnlI CCTC 8 cut(s) 21, 28, 210, 411, 414, 575, 673, 686
MroXI GAANNNNTTC 1 cut(s) 325
MseI TTAA 5 cut(s) 423, 468, 636, 663, 735
MslI CAYNNNNRTG 2 cut(s) 469, 776
MspI CCGG 1 cut(s) 535
MunI CAATTG 1 cut(s) 213
MvnI CGCG 1 cut(s) 720
MwoI GCNNNNNNNGC 2 cut(s) 89, 461
NcoI CCATGG 1 cut(s) 205
NdeI CATATG 2 cut(s) 484, 825
NdeII GATC 2 cut(s) 356, 622
NlaIII CATG 2 cut(s) 209, 273
NspV TTCGAA 1 cut(s) 415
PaeR7I CTCGAG 1 cut(s) 314
PciSI GCTCTTC 1 cut(s) 316
PdmI GAANNNNTTC 1 cut(s) 325
PflMI CCANNNNNTGG 1 cut(s) 263
Ppu21I YACGTR 1 cut(s) 461
PsiI TTATAA 1 cut(s) 242
PspFI CCCAGC 1 cut(s) 350
PsuI RGATCY 1 cut(s) 356
RsaI GTAC 1 cut(s) 211
RsaNI GTAC 1 cut(s) 210
RseI CAYNNNNRTG 2 cut(s) 469, 776
SapI GCTCTTC 1 cut(s) 316
SaqAI TTAA 5 cut(s) 423, 468, 636, 663, 735
Sau3AI GATC 2 cut(s) 356, 622
SduI GDGCHC 1 cut(s) 655
SfaNI GCATC 2 cut(s) 376, 650
Sfr274I CTCGAG 1 cut(s) 314
SfuI TTCGAA 1 cut(s) 415
SlaI CTCGAG 1 cut(s) 314
SmiI ATTTAAAT 1 cut(s) 469
SmiMI CAYNNNNRTG 2 cut(s) 469, 776
SmlI CTYRAG 2 cut(s) 314, 654
SmoI CTYRAG 2 cut(s) 314, 654
Sse9I AATT 7 cut(s) 148, 156, 175, 213, 443, 496, 796
SsiI CCGC 1 cut(s) 809
StyI CCWWGG 2 cut(s) 205, 710
SwaI ATTTAAAT 1 cut(s) 469
TaaI ACNGT 3 cut(s) 132, 247, 431
TaiI ACGT 1 cut(s) 463
TaqI TCGA 2 cut(s) 315, 415
TasI AATT 7 cut(s) 148, 156, 175, 213, 443, 496, 796
Tru1I TTAA 5 cut(s) 423, 468, 636, 663, 735
Tru9I TTAA 5 cut(s) 423, 468, 636, 663, 735
TspDTI ATGAA 5 cut(s) 248, 258, 436, 561, 620
Van91I CCANNNNNTGG 1 cut(s) 263
XapI RAATTY 1 cut(s) 443
XcmI CCANNNNNNNNNTGG 1 cut(s) 203
XhoI CTCGAG 1 cut(s) 314
XmnI GAANNNNTTC 1 cut(s) 325
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.