AT4G27540

May be involved in both secretory and endocytic intracellular trafficking in the endosomal prevacuolar compartments

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0008479
Physical Location & Seq
Reverse (-)
13753210 .. 13754812
1603 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G27540.2

Sequence Viewer

Length: 726 bp
ATGGCTTTCTCTCCTAATCCTCTCTCTCTAAGCGTCCCCGATCCTGCTTTCGAATCTTGGCTTCGAGATTCCGGTTACCTCGAGCTCCTCGATCACCGCACCTCCGCCGCTGCAGCTGCCGCTTCCTCCTCCGCCTCCGTTTCATCCTCAGCTGCCGCAACTTCTGCGGCATCTGACGATGTCGTTTCATCCATTACCGGTGGTTTCTTCGCTTCTCTCTTATCTCGACTCGTCACTGTCTCTTCTCTTCTTACTATTAACCCTTTCTCTAAGCTCTCCGCCGATGATTTTTCCGGAGATACGACACCGTGGACCACCGGTTTCATTGGCAATTGTGACTCTTATTCGTTCCCGTCGTCTTCTCAGCAAGCGAGAATGAGAGTTCATGAGAATATCAAACGATTCGCTAGGAATTACGCTACGTTGTTCATCGTCTTCTTTGCTTGTGCACTATATCAGATGCCACTTGCATTGGTGGGATTGTTAGGAAGCTTAGCTCTTTGGGAGCTATTCAAATACTGCAGTGATAAGTGGAAATTTGATCGTCACCCTTCCATGAGGAAACTCTCGATTGGTATTGGACAATGCGCAACAGCGGTTCTTCTAACGTTTTTGAATGTCCAAATGGCTCTTTTCTCTGCTCTTGCGATCAGTTATTCAGTTATGATATTGCATGCTGGGTTCAGAAAGCTCACGCCATCCAAGAAACCAACTCGAGGAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006810 GO:0008150 GO:0012505 GO:0016192 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0051179 GO:0051234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PRA1

Protein Analysis

241

Amino Acids

26.07

Weight (kDa)

9.27

Isoelectric Point (pI)

34.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRA1 PF03208 103 - 174 3.6e-10 PRA1 family protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 589
AccIII TCCGGA 1 cut(s) 293
AciI CCGC 9 cut(s) 97, 105, 108, 120, 132, 156, 167, 279, 596
AclI AACGTT 1 cut(s) 608
AclWI GGATC 1 cut(s) 35
AcsI RAATTY 1 cut(s) 536
AfiI CCNNNNNNNGG 1 cut(s) 716
AgeI ACCGGT 2 cut(s) 197, 317
AgsI TTSAA 2 cut(s) 514, 616
AluBI AGCT 8 cut(s) 85, 116, 152, 274, 492, 497, 508, 691
AluI AGCT 8 cut(s) 85, 116, 152, 274, 492, 497, 508, 691
Alw21I GWGCWC 2 cut(s) 87, 451
Alw26I GTCTC 1 cut(s) 244
Alw44I GTGCAC 1 cut(s) 447
AlwI GGATC 1 cut(s) 35
Ama87I CYCGRG 2 cut(s) 80, 714
Aor13HI TCCGGA 1 cut(s) 293
ApaLI GTGCAC 1 cut(s) 447
ApeKI GCWGC 4 cut(s) 110, 113, 116, 152
ApoI RAATTY 1 cut(s) 536
AsiGI ACCGGT 2 cut(s) 197, 317
AspLEI GCGC 1 cut(s) 590
AspS9I GGNCC 1 cut(s) 312
AsuHPI GGTGA 2 cut(s) 86, 539
AsuII TTCGAA 1 cut(s) 51
AvaI CYCGRG 2 cut(s) 80, 714
AvaII GGWCC 1 cut(s) 312
BaeGI GKGCMC 1 cut(s) 451
BanII GRGCYC 1 cut(s) 87
BbsI GAAGAC 2 cut(s) 351, 427
Bbv12I GWGCWC 2 cut(s) 87, 451
BbvCI CCTCAGC 1 cut(s) 148
BbvI GCAGC 4 cut(s) 97, 103, 125, 139
BccI CCATC 1 cut(s) 706
BcoDI GTCTC 1 cut(s) 244
BfaI CTAG 1 cut(s) 408
BfmI CTRYAG 2 cut(s) 111, 520
BisI GCNGC 8 cut(s) 108, 111, 114, 117, 120, 153, 156, 168
BlpI GCTNAGC 1 cut(s) 493
BlsI GCNGC 8 cut(s) 109, 112, 115, 118, 121, 154, 157, 169
Bme18I GGWCC 1 cut(s) 312
BmeT110I CYCGRG 2 cut(s) 80, 714
BmgT120I GGNCC 1 cut(s) 312
BmsI GCATC 2 cut(s) 179, 450
BpiI GAAGAC 2 cut(s) 351, 427
Bpu10I CCTNAGC 1 cut(s) 148
Bpu1102I GCTNAGC 1 cut(s) 493
Bpu14I TTCGAA 1 cut(s) 51
BsaJI CCNNGG 1 cut(s) 308
BsaWI WCCGGW 4 cut(s) 71, 197, 293, 317
BsaXI ACNNNNNCTCC 2 cut(s) 86, 116
Bsc4I CCNNNNNNNGG 1 cut(s) 716
Bse118I RCCGGY 2 cut(s) 197, 317
BseAI TCCGGA 1 cut(s) 293
BseDI CCNNGG 1 cut(s) 308
BseGI GGATG 3 cut(s) 143, 188, 698
BseLI CCNNNNNNNGG 1 cut(s) 716
BseMII CTCAG 2 cut(s) 162, 377
BseRI GAGGAG 2 cut(s) 77, 118
BseSI GKGCMC 1 cut(s) 451
BseXI GCAGC 4 cut(s) 97, 103, 125, 139
BseYI CCCAGC 1 cut(s) 677
BshTI ACCGGT 2 cut(s) 197, 317
BsiHKAI GWGCWC 2 cut(s) 87, 451
BsiHKCI CYCGRG 2 cut(s) 80, 714
BsiSI CCGG 4 cut(s) 72, 198, 294, 318
BslFI GGGAC 1 cut(s) 20
BslI CCNNNNNNNGG 1 cut(s) 716
BsmAI GTCTC 1 cut(s) 244
BsmFI GGGAC 1 cut(s) 20
BsoBI CYCGRG 2 cut(s) 80, 714
Bsp119I TTCGAA 1 cut(s) 51
Bsp1286I GDGCHC 2 cut(s) 87, 451
Bsp13I TCCGGA 1 cut(s) 293
Bsp143I GATC 4 cut(s) 40, 91, 541, 648
Bsp1720I GCTNAGC 1 cut(s) 493
BspACI CCGC 9 cut(s) 97, 105, 108, 120, 132, 156, 167, 279, 596
BspCNI CTCAG 2 cut(s) 161, 376
BspEI TCCGGA 1 cut(s) 293
BspHI TCATGA 1 cut(s) 385
BspMAI CTGCAG 2 cut(s) 115, 524
BspPI GGATC 1 cut(s) 35
BspT104I TTCGAA 1 cut(s) 51
BsrFI RCCGGY 2 cut(s) 197, 317
BssAI RCCGGY 2 cut(s) 197, 317
BssECI CCNNGG 1 cut(s) 308
BssMI GATC 4 cut(s) 40, 91, 541, 648
Bst4CI ACNGT 2 cut(s) 238, 309
Bst6I CTCTTC 2 cut(s) 247, 252
BstAPI GCANNNNNTGC 1 cut(s) 164
BstBI TTCGAA 1 cut(s) 51
BstC8I GCNNGC 2 cut(s) 369, 675
BstDEI CTNAG 5 cut(s) 29, 148, 270, 363, 493
BstDSI CCRYGG 1 cut(s) 308
BstEII GGTNACC 1 cut(s) 74
BstF5I GGATG 3 cut(s) 143, 188, 698
BstHHI GCGC 1 cut(s) 590
BstKTI GATC 4 cut(s) 43, 94, 544, 651
BstMAI GTCTC 1 cut(s) 244
BstMBI GATC 4 cut(s) 40, 91, 541, 648
BstMWI GCNNNNNNNGC 4 cut(s) 113, 116, 119, 164
BstNSI RCATGY 1 cut(s) 677
BstPI GGTNACC 1 cut(s) 74
BstSFI CTRYAG 2 cut(s) 111, 520
BstSLI GKGCMC 1 cut(s) 451
BstV1I GCAGC 4 cut(s) 97, 103, 125, 139
BstV2I GAAGAC 2 cut(s) 351, 427
BtgI CCRYGG 1 cut(s) 308
BtsCI GGATG 3 cut(s) 143, 188, 698
BtsI GCAGTG 1 cut(s) 529
BtsIMutI CAGTG 2 cut(s) 234, 529
Cac8I GCNNGC 2 cut(s) 369, 675
CciI TCATGA 1 cut(s) 385
CfoI GCGC 1 cut(s) 590
Cfr10I RCCGGY 2 cut(s) 197, 317
Cfr13I GGNCC 1 cut(s) 312
CseI GACGC 1 cut(s) 22
CspAI ACCGGT 2 cut(s) 197, 317
CspCI CAANNNNNGTGG 2 cut(s) 453, 488
CviAII CATG 3 cut(s) 386, 556, 674
DdeI CTNAG 5 cut(s) 29, 148, 270, 363, 493
DpnI GATC 4 cut(s) 42, 93, 543, 650
DpnII GATC 4 cut(s) 40, 91, 541, 648
Eam1104I CTCTTC 2 cut(s) 247, 252
EarI CTCTTC 2 cut(s) 247, 252
EciI GGCGGA 3 cut(s) 94, 121, 268
Ecl136II GAGCTC 1 cut(s) 85
Eco24I GRGCYC 1 cut(s) 87
Eco47I GGWCC 1 cut(s) 312
Eco53kI GAGCTC 1 cut(s) 85
Eco88I CYCGRG 2 cut(s) 80, 714
Eco91I GGTNACC 1 cut(s) 74
EcoICRI GAGCTC 1 cut(s) 85
EcoO65I GGTNACC 1 cut(s) 74
EcoT38I GRGCYC 1 cut(s) 87
FaeI CATG 3 cut(s) 389, 559, 677
FaiI YATR 5 cut(s) 387, 454, 557, 665, 675
FaqI GGGAC 1 cut(s) 20
FatI CATG 3 cut(s) 385, 555, 673
Fnu4HI GCNGC 8 cut(s) 108, 111, 114, 117, 120, 153, 156, 168
FokI GGATG 3 cut(s) 130, 175, 685
FriOI GRGCYC 1 cut(s) 87
Fsp4HI GCNGC 8 cut(s) 108, 111, 114, 117, 120, 153, 156, 168
FspBI CTAG 1 cut(s) 408
FspI TGCGCA 1 cut(s) 589
GlaI GCGC 1 cut(s) 589
GluI GCNGC 8 cut(s) 108, 111, 114, 117, 120, 153, 156, 168
GsaI CCCAGC 1 cut(s) 681
HapII CCGG 4 cut(s) 72, 198, 294, 318
HgaI GACGC 1 cut(s) 22
HhaI GCGC 1 cut(s) 590
Hin1II CATG 3 cut(s) 389, 559, 677
Hin6I GCGC 1 cut(s) 588
HinP1I GCGC 1 cut(s) 588
HindIII AAGCTT 1 cut(s) 490
HinfI GANTC 5 cut(s) 53, 68, 228, 338, 402
HpaII CCGG 4 cut(s) 72, 198, 294, 318
HphI GGTGA 2 cut(s) 86, 539
Hpy166II GTNNAC 2 cut(s) 312, 449
Hpy188I TCNGA 3 cut(s) 175, 459, 686
Hpy188III TCNNGA 5 cut(s) 65, 225, 294, 386, 568
Hpy8I GTNNAC 2 cut(s) 312, 449
Hpy99I CGWCG 1 cut(s) 358
HpyAV CCTTC 1 cut(s) 561
HpyCH4III ACNGT 2 cut(s) 238, 309
HpyCH4IV ACGT 2 cut(s) 422, 608
HpyCH4V TGCA 5 cut(s) 113, 449, 470, 522, 673
HpyF10VI GCNNNNNNNGC 4 cut(s) 113, 116, 119, 164
HpyF3I CTNAG 5 cut(s) 29, 148, 270, 363, 493
HpySE526I ACGT 2 cut(s) 422, 608
Hsp92II CATG 3 cut(s) 389, 559, 677
HspAI GCGC 1 cut(s) 588
Kpn2I TCCGGA 1 cut(s) 293
Kzo9I GATC 4 cut(s) 40, 91, 541, 648
LmnI GCTCC 2 cut(s) 90, 505
LpnPI CCDG 6 cut(s) 57, 85, 211, 307, 331, 663
Lsp1109I GCAGC 4 cut(s) 97, 103, 125, 139
LweI GCATC 2 cut(s) 179, 450
MaeI CTAG 1 cut(s) 408
MaeII ACGT 2 cut(s) 422, 608
MaeIII GTNAC 4 cut(s) 74, 232, 335, 545
MalI GATC 4 cut(s) 42, 93, 543, 650
MboI GATC 4 cut(s) 40, 91, 541, 648
MboII GAAGA 6 cut(s) 199, 234, 239, 351, 427, 593
MfeI CAATTG 1 cut(s) 331
MhlI GDGCHC 2 cut(s) 87, 451
MluCI AATT 3 cut(s) 331, 412, 536
MlyI GAGTC 2 cut(s) 222, 332
MroI TCCGGA 1 cut(s) 293
MseI TTAA 1 cut(s) 258
MspA1I CMGCKG 4 cut(s) 110, 116, 152, 596
MspI CCGG 4 cut(s) 72, 198, 294, 318
MunI CAATTG 1 cut(s) 331
MwoI GCNNNNNNNGC 4 cut(s) 113, 116, 119, 164
NdeII GATC 4 cut(s) 40, 91, 541, 648
NlaIII CATG 3 cut(s) 389, 559, 677
NmuCI GTSAC 3 cut(s) 232, 335, 545
NsbI TGCGCA 1 cut(s) 589
NspI RCATGY 1 cut(s) 677
NspV TTCGAA 1 cut(s) 51
PaeI GCATGC 1 cut(s) 677
PaeR7I CTCGAG 2 cut(s) 80, 714
PagI TCATGA 1 cut(s) 385
PcsI WCGNNNNNNNCGW 2 cut(s) 87, 353
PfeI GAWTC 3 cut(s) 53, 68, 402
PflFI GACNNNGTC 1 cut(s) 179
PinAI ACCGGT 2 cut(s) 197, 317
PkrI GCNGC 8 cut(s) 109, 112, 115, 118, 121, 154, 157, 169
PleI GAGTC 2 cut(s) 222, 332
PpsI GAGTC 2 cut(s) 222, 332
Psp124BI GAGCTC 1 cut(s) 87
Psp1406I AACGTT 1 cut(s) 608
PspEI GGTNACC 1 cut(s) 74
PspFI CCCAGC 1 cut(s) 677
PspPI GGNCC 1 cut(s) 312
PspXI VCTCGAGB 2 cut(s) 80, 714
PstI CTGCAG 2 cut(s) 115, 524
PsyI GACNNNGTC 1 cut(s) 179
PvuII CAGCTG 2 cut(s) 116, 152
SacI GAGCTC 1 cut(s) 87
SaqAI TTAA 1 cut(s) 258
SatI GCNGC 8 cut(s) 108, 111, 114, 117, 120, 153, 156, 168
Sau3AI GATC 4 cut(s) 40, 91, 541, 648
Sau96I GGNCC 1 cut(s) 312
SchI GAGTC 2 cut(s) 222, 332
SduI GDGCHC 2 cut(s) 87, 451
SfaNI GCATC 2 cut(s) 179, 450
SfcI CTRYAG 2 cut(s) 111, 520
Sfr274I CTCGAG 2 cut(s) 80, 714
SfuI TTCGAA 1 cut(s) 51
SinI GGWCC 1 cut(s) 312
SlaI CTCGAG 2 cut(s) 80, 714
SmlI CTYRAG 2 cut(s) 80, 714
SmoI CTYRAG 2 cut(s) 80, 714
SphI GCATGC 1 cut(s) 677
Sse9I AATT 3 cut(s) 331, 412, 536
SsiI CCGC 9 cut(s) 97, 105, 108, 120, 132, 156, 167, 279, 596
SspMI CTAG 1 cut(s) 408
SstI GAGCTC 1 cut(s) 87
TaaI ACNGT 2 cut(s) 238, 309
TaiI ACGT 2 cut(s) 425, 611
TaqI TCGA 7 cut(s) 51, 64, 81, 90, 226, 569, 715
TasI AATT 3 cut(s) 331, 412, 536
TauI GCSGC 4 cut(s) 110, 122, 158, 170
TfiI GAWTC 3 cut(s) 53, 68, 402
Tru1I TTAA 1 cut(s) 258
Tru9I TTAA 1 cut(s) 258
TscAI CASTG 2 cut(s) 241, 529
TseFI GTSAC 3 cut(s) 232, 335, 545
TseI GCWGC 4 cut(s) 110, 113, 116, 152
Tsp45I GTSAC 3 cut(s) 232, 335, 545
TspDTI ATGAA 5 cut(s) 132, 177, 313, 374, 418
TspGWI ACGGA 1 cut(s) 127
TspRI CASTG 2 cut(s) 241, 529
Tth111I GACNNNGTC 1 cut(s) 179
VneI GTGCAC 1 cut(s) 447
VpaK11BI GGWCC 1 cut(s) 312
XapI RAATTY 1 cut(s) 536
XceI RCATGY 1 cut(s) 677
XhoI CTCGAG 2 cut(s) 80, 714
XspI CTAG 1 cut(s) 408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.