MD03G1231400.v1.1

May be involved in both secretory and endocytic intracellular trafficking in the endosomal prevacuolar compartments

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0008479
Physical Location & Seq
Reverse (-)
31698220 .. 31702255
4036 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1231400.v1.1.491

Sequence Viewer

Length: 852 bp
ATGATTTTATTCATATTTCACACTCTTTGTACGACCAATATAATTCAAGGAAAAACTTTTATCTTGTCAACGAAACAAACCAAGATAACATCATCATCACAAAAATCCCAAAATCCAAAGCCAAACCAAAAAGTTGCAGGTAGGACTCGGGAGGGAATTACCATGGTATTCACCTCCAACCCTTTATCTCTCAGCGTCCCCGACCCTTTCTTCGAGTCATGGCTCCGCGACAATGGCTACCCAGAAATCATCGACCAGCACACCTCCACAGCCATCACCACCTCCTCCGCAGCCACCGCTGACGCAGCCTCGATCACAAACGGCTTCTTCATCTCACTCTTCTCTCGCATTTTCACCCTTTTCTCTCTCTTCACCATTAACCCCTTCTCCAAGCTCACCAACGATGACCTTGCCTCCCAGACGCCGCCCTGGACCACCTCCTTTTTTGGGTCCTCTGAATCCTACTCTTTCCCTCTCTCTGTTTCTCAGGCTCGCATGAGGGTGCAGGAGAATGTCAAGCGCTATGCTAGAAATTATGCTACTTTGTTCGTGCTCTTCTTTGCTTGCTCGTTATATCGAATGCCACTGGCTCTTGTTGGATTGATGTCTTGTCTGGCACTATGGGATGTTTTTAAGTTCTGTAGTGAAAGGTGGGGACTGGACCAATACCCGATAGTTCGGCAGTGCTTAATTCGTGTTGCTCAATGTGTAGCTGCGGTTATCCTGATATGTTCAAATGTTCAAATGGCTATCTTCTGTGCTCTTACCGTCAGTTATGCAGGCATGATTCTGCATGCTGGATTTCGGAAGCTGACTAGTGCAAAGCAAACTCCTCGAGGTCGATCAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006810 GO:0008150 GO:0012505 GO:0016192 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0051179 GO:0051234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PRA1

Protein Analysis

284

Amino Acids

31.63

Weight (kDa)

9.61

Isoelectric Point (pI)

38.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRA1 PF03208 143 - 270 4.9e-17 PRA1 family protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 834
Acc36I ACCTGC 1 cut(s) 128
AccII CGCG 1 cut(s) 228
AciI CCGC 5 cut(s) 226, 288, 297, 425, 716
AcyI GRCGYC 1 cut(s) 422
AfaI GTAC 1 cut(s) 31
AfeI AGCGCT 1 cut(s) 521
AfiI CCNNNNNNNGG 1 cut(s) 447
AgsI TTSAA 3 cut(s) 47, 735, 743
AhlI ACTAGT 1 cut(s) 815
AjnI CCWGG 1 cut(s) 428
AluBI AGCT 3 cut(s) 394, 713, 811
AluI AGCT 3 cut(s) 394, 713, 811
Alw21I GWGCWC 2 cut(s) 555, 763
Ama87I CYCGRG 2 cut(s) 147, 834
Aor51HI AGCGCT 1 cut(s) 521
ApeKI GCWGC 3 cut(s) 290, 305, 713
ArsI GACNNNNNNTTYG 2 cut(s) 194, 226
AspLEI GCGC 1 cut(s) 522
AspS9I GGNCC 3 cut(s) 432, 450, 661
AsuHPI GGTGA 5 cut(s) 163, 268, 346, 364, 388
AvaI CYCGRG 2 cut(s) 147, 834
AvaII GGWCC 3 cut(s) 432, 450, 661
Bbv12I GWGCWC 2 cut(s) 555, 763
BbvI GCAGC 3 cut(s) 302, 317, 700
BccI CCATC 1 cut(s) 281
BceAI ACGGC 1 cut(s) 337
BcgI CGANNNNNNTGC 4 cut(s) 392, 426, 815, 849
BciT130I CCWGG 1 cut(s) 430
BcuI ACTAGT 1 cut(s) 815
BfaI CTAG 2 cut(s) 528, 816
BfmI CTRYAG 1 cut(s) 640
BfoI RGCGCY 1 cut(s) 523
BfuAI ACCTGC 1 cut(s) 128
BisI GCNGC 4 cut(s) 291, 306, 425, 714
BlsI GCNGC 4 cut(s) 292, 307, 426, 715
Bme1390I CCNGG 1 cut(s) 430
Bme18I GGWCC 3 cut(s) 432, 450, 661
BmeT110I CYCGRG 2 cut(s) 147, 834
BmgT120I GGNCC 3 cut(s) 432, 450, 661
BmiI GGNNCC 2 cut(s) 224, 451
BmrFI CCNGG 1 cut(s) 430
BsaHI GRCGYC 1 cut(s) 422
BsaJI CCNNGG 2 cut(s) 162, 428
BsaXI ACNNNNNCTCC 4 cut(s) 371, 398, 401, 428
Bsc4I CCNNNNNNNGG 1 cut(s) 447
Bse1I ACTGG 2 cut(s) 591, 663
BseBI CCWGG 1 cut(s) 430
BseDI CCNNGG 2 cut(s) 162, 428
BseGI GGATG 1 cut(s) 631
BseLI CCNNNNNNNGG 1 cut(s) 447
BseMII CTCAG 2 cut(s) 205, 500
BseNI ACTGG 2 cut(s) 591, 663
BseRI GAGGAG 2 cut(s) 274, 822
BseXI GCAGC 3 cut(s) 302, 317, 700
BsgI GTGCAG 1 cut(s) 524
Bsh1236I CGCG 1 cut(s) 228
BsiHKAI GWGCWC 2 cut(s) 555, 763
BsiHKCI CYCGRG 2 cut(s) 147, 834
BslFI GGGAC 2 cut(s) 182, 669
BslI CCNNNNNNNGG 1 cut(s) 447
BsmFI GGGAC 2 cut(s) 182, 669
BsmI GAATGC 1 cut(s) 585
BsoBI CYCGRG 2 cut(s) 147, 834
Bsp1286I GDGCHC 2 cut(s) 555, 763
Bsp143I GATC 2 cut(s) 312, 842
Bsp19I CCATGG 1 cut(s) 162
BspACI CCGC 5 cut(s) 226, 288, 297, 425, 716
BspCNI CTCAG 2 cut(s) 204, 499
BspFNI CGCG 1 cut(s) 228
BspLI GGNNCC 2 cut(s) 224, 451
BspMI ACCTGC 1 cut(s) 128
BspQI GCTCTTC 1 cut(s) 560
BsrI ACTGG 2 cut(s) 591, 663
BssECI CCNNGG 2 cut(s) 162, 428
BssMI GATC 2 cut(s) 312, 842
BssNI GRCGYC 1 cut(s) 422
BssT1I CCWWGG 1 cut(s) 162
Bst2UI CCWGG 1 cut(s) 430
Bst4CI ACNGT 1 cut(s) 769
Bst6I CTCTTC 3 cut(s) 344, 374, 560
BstACI GRCGYC 1 cut(s) 422
BstC8I GCNNGC 4 cut(s) 493, 565, 781, 795
BstDEI CTNAG 2 cut(s) 191, 486
BstDSI CCRYGG 1 cut(s) 162
BstF5I GGATG 1 cut(s) 631
BstFNI CGCG 1 cut(s) 228
BstH2I RGCGCY 1 cut(s) 523
BstHHI GCGC 1 cut(s) 522
BstKTI GATC 2 cut(s) 315, 845
BstMBI GATC 2 cut(s) 312, 842
BstMWI GCNNNNNNNGC 3 cut(s) 234, 296, 305
BstNI CCWGG 1 cut(s) 430
BstNSI RCATGY 1 cut(s) 797
BstSCI CCNGG 1 cut(s) 428
BstSFI CTRYAG 1 cut(s) 640
BstUI CGCG 1 cut(s) 228
BstV1I GCAGC 3 cut(s) 302, 317, 700
BtgI CCRYGG 1 cut(s) 162
BtsCI GGATG 1 cut(s) 631
BtsI GCAGTG 1 cut(s) 689
BtsIMutI CAGTG 2 cut(s) 584, 689
BveI ACCTGC 1 cut(s) 128
Cac8I GCNNGC 4 cut(s) 493, 565, 781, 795
CfoI GCGC 1 cut(s) 522
Cfr13I GGNCC 3 cut(s) 432, 450, 661
CseI GACGC 3 cut(s) 184, 311, 430
Csp6I GTAC 1 cut(s) 30
CviAII CATG 5 cut(s) 163, 219, 496, 784, 794
CviQI GTAC 1 cut(s) 30
DdeI CTNAG 2 cut(s) 191, 486
DpnI GATC 2 cut(s) 314, 844
DpnII GATC 2 cut(s) 312, 842
Eam1104I CTCTTC 3 cut(s) 344, 374, 560
EarI CTCTTC 3 cut(s) 344, 374, 560
Eco130I CCWWGG 1 cut(s) 162
Eco47I GGWCC 3 cut(s) 432, 450, 661
Eco47III AGCGCT 1 cut(s) 521
Eco88I CYCGRG 2 cut(s) 147, 834
EcoO109I RGGNCCY 1 cut(s) 450
EcoRII CCWGG 1 cut(s) 428
EcoT14I CCWWGG 1 cut(s) 162
ErhI CCWWGG 1 cut(s) 162
FaeI CATG 5 cut(s) 166, 222, 499, 787, 797
FaqI GGGAC 2 cut(s) 182, 669
FatI CATG 5 cut(s) 162, 218, 495, 783, 793
Fnu4HI GCNGC 4 cut(s) 291, 306, 425, 714
FokI GGATG 1 cut(s) 638
Fsp4HI GCNGC 4 cut(s) 291, 306, 425, 714
FspBI CTAG 2 cut(s) 528, 816
GlaI GCGC 1 cut(s) 521
GluI GCNGC 4 cut(s) 291, 306, 425, 714
HaeII RGCGCY 1 cut(s) 523
HgaI GACGC 3 cut(s) 184, 311, 430
HhaI GCGC 1 cut(s) 522
Hin1I GRCGYC 1 cut(s) 422
Hin1II CATG 5 cut(s) 166, 222, 499, 787, 797
Hin6I GCGC 1 cut(s) 520
HinP1I GCGC 1 cut(s) 520
HincII GTYRAC 1 cut(s) 69
HindII GTYRAC 1 cut(s) 69
HinfI GANTC 4 cut(s) 145, 215, 458, 787
HphI GGTGA 5 cut(s) 163, 268, 346, 364, 388
Hpy166II GTNNAC 1 cut(s) 69
Hpy188I TCNGA 2 cut(s) 457, 807
Hpy188III TCNNGA 2 cut(s) 149, 724
Hpy8I GTNNAC 1 cut(s) 69
HpyAV CCTTC 1 cut(s) 394
HpyCH4III ACNGT 1 cut(s) 769
HpyCH4V TGCA 5 cut(s) 137, 505, 779, 793, 821
HpyF10VI GCNNNNNNNGC 3 cut(s) 234, 296, 305
HpyF3I CTNAG 2 cut(s) 191, 486
Hsp92I GRCGYC 1 cut(s) 422
Hsp92II CATG 5 cut(s) 166, 222, 499, 787, 797
HspAI GCGC 1 cut(s) 520
Kzo9I GATC 2 cut(s) 312, 842
LguI GCTCTTC 1 cut(s) 560
LmnI GCTCC 1 cut(s) 228
Lsp1109I GCAGC 3 cut(s) 302, 317, 700
MaeI CTAG 2 cut(s) 528, 816
MalI GATC 2 cut(s) 314, 844
MboI GATC 2 cut(s) 312, 842
MboII GAAGA 6 cut(s) 202, 319, 331, 361, 547, 745
MhlI GDGCHC 2 cut(s) 555, 763
MluCI AATT 4 cut(s) 42, 156, 532, 690
MlyI GAGTC 2 cut(s) 139, 224
MmeI TCCRAC 2 cut(s) 201, 577
MseI TTAA 3 cut(s) 378, 633, 689
MslI CAYNNNNRTG 1 cut(s) 500
MspA1I CMGCKG 1 cut(s) 299
MspR9I CCNGG 1 cut(s) 430
Mva1269I GAATGC 1 cut(s) 585
MvaI CCWGG 1 cut(s) 430
MvnI CGCG 1 cut(s) 228
MwoI GCNNNNNNNGC 3 cut(s) 234, 296, 305
NcoI CCATGG 1 cut(s) 162
NdeII GATC 2 cut(s) 312, 842
NlaIII CATG 5 cut(s) 166, 222, 499, 787, 797
NlaIV GGNNCC 2 cut(s) 224, 451
NspI RCATGY 1 cut(s) 797
PaeI GCATGC 1 cut(s) 797
PaeR7I CTCGAG 1 cut(s) 834
PciSI GCTCTTC 1 cut(s) 560
PctI GAATGC 1 cut(s) 585
PfeI GAWTC 2 cut(s) 458, 787
PkrI GCNGC 4 cut(s) 292, 307, 426, 715
PleI GAGTC 2 cut(s) 139, 223
PpsI GAGTC 2 cut(s) 139, 223
PpuMI RGGWCCY 1 cut(s) 450
Psp5II RGGWCCY 1 cut(s) 450
Psp6I CCWGG 1 cut(s) 428
PspGI CCWGG 1 cut(s) 428
PspN4I GGNNCC 2 cut(s) 224, 451
PspPI GGNCC 3 cut(s) 432, 450, 661
PspPPI RGGWCCY 1 cut(s) 450
PspXI VCTCGAGB 1 cut(s) 834
RsaI GTAC 1 cut(s) 31
RsaNI GTAC 1 cut(s) 30
RseI CAYNNNNRTG 1 cut(s) 500
SapI GCTCTTC 1 cut(s) 560
SaqAI TTAA 3 cut(s) 378, 633, 689
SatI GCNGC 4 cut(s) 291, 306, 425, 714
Sau3AI GATC 2 cut(s) 312, 842
Sau96I GGNCC 3 cut(s) 432, 450, 661
SchI GAGTC 2 cut(s) 139, 224
ScrFI CCNGG 1 cut(s) 430
SduI GDGCHC 2 cut(s) 555, 763
SfcI CTRYAG 1 cut(s) 640
Sfr274I CTCGAG 1 cut(s) 834
SinI GGWCC 3 cut(s) 432, 450, 661
SlaI CTCGAG 1 cut(s) 834
SmiMI CAYNNNNRTG 1 cut(s) 500
SmlI CTYRAG 1 cut(s) 834
SmoI CTYRAG 1 cut(s) 834
SpeI ACTAGT 1 cut(s) 815
SphI GCATGC 1 cut(s) 797
Sse9I AATT 4 cut(s) 42, 156, 532, 690
SsiI CCGC 5 cut(s) 226, 288, 297, 425, 716
SspMI CTAG 2 cut(s) 528, 816
StyD4I CCNGG 1 cut(s) 428
StyI CCWWGG 1 cut(s) 162
TaaI ACNGT 1 cut(s) 769
TaqI TCGA 6 cut(s) 213, 252, 311, 577, 835, 841
TasI AATT 4 cut(s) 42, 156, 532, 690
TauI GCSGC 1 cut(s) 427
TfiI GAWTC 2 cut(s) 458, 787
Tru1I TTAA 3 cut(s) 378, 633, 689
Tru9I TTAA 3 cut(s) 378, 633, 689
TscAI CASTG 2 cut(s) 591, 689
TseI GCWGC 3 cut(s) 290, 305, 713
TspDTI ATGAA 1 cut(s) 319
TspRI CASTG 2 cut(s) 591, 689
VpaK11BI GGWCC 3 cut(s) 432, 450, 661
XceI RCATGY 1 cut(s) 797
XhoI CTCGAG 1 cut(s) 834
XspI CTAG 2 cut(s) 528, 816
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.