Rw5G020750

May be involved in both secretory and endocytic intracellular trafficking in the endosomal prevacuolar compartments

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0008479
Physical Location & Seq
Forward (+)
27468901 .. 27471683
2783 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G020750.1

Sequence Viewer

Length: 720 bp
ATGGTATTCTCAGCCAACCCATTATCCCTCAGCGTCTCCGACCCTTTCTTCGACTCATGGCTCCGTCAAAACGGCTACCCTGAAATCCTCGAGCACCGACCCACCTCCACCACACCCACCACCGTCTCCGCCGCCACAACCTCCACCTCCGTCGGCACCTCAACCTCCACCTCCATCTCCACTGCAAACGGGTTCTTCACCTCACTCTACTCCTCCGTCTGCATCCTCCTCTCTCTCTTCGCCACCAACCCCTTCGCCAAGCTCAGCAACGAGGACCTCGCCGTCCCTACGCCGCCGTGGCAAGCCGGGTTTATTGGCGACTACGAGTCCTACTCGTTCCCGGTGTCTAGCTCTCAGGCCCAGTTGAGGGTCCAGGAGAATGTGAAGAGATATGCTAGGAGCTATGCGTGGTTGTTGGTCATCTTCTTCGCTTGTTCTCTATATCAAATGCCATTGGCTGCTGCTGGATTGATATTTTGTTTGGCACTATGGGAAATCTTTAAGCGCTGCGGTGAAATGTGGGGACTGGATCAGTACCCAACAATTCAGCAAACCATAATACGTGTTGCTCAATGTGTAGCTGCTGTTATCCTGATATATTCGGGTGTTCAAATGGCTCTATTCTCGGCACTTAGTGTCACTTATGCAGGAATGATACTACACGCCGGATTTCGGACGCTGACTCGTGCAAAGCAAGCTTCTCGTGGTAGATCGAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006810 GO:0008150 GO:0012505 GO:0016192 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0051179 GO:0051234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PRA1

Protein Analysis

239

Amino Acids

26.26

Weight (kDa)

8.85

Isoelectric Point (pI)

43.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRA1 PF03208 99 - 226 9.4e-13 PRA1 family protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 281
AccB1I GGYRCC 1 cut(s) 155
AciI CCGC 4 cut(s) 129, 132, 293, 510
AclWI GGATC 1 cut(s) 537
AdeI CACNNNGTG 1 cut(s) 635
AfaI GTAC 1 cut(s) 536
AfeI AGCGCT 1 cut(s) 506
AfiI CCNNNNNNNGG 3 cut(s) 366, 367, 672
AflIII ACRYGT 1 cut(s) 562
AgsI TTSAA 1 cut(s) 611
AhdI GACNNNNNGTC 1 cut(s) 325
AjnI CCWGG 1 cut(s) 372
AluBI AGCT 5 cut(s) 262, 351, 402, 581, 698
AluI AGCT 5 cut(s) 262, 351, 402, 581, 698
Alw21I GWGCWC 1 cut(s) 96
Alw26I GTCTC 2 cut(s) 40, 130
AlwI GGATC 1 cut(s) 537
Ama87I CYCGRG 1 cut(s) 89
Aor51HI AGCGCT 1 cut(s) 506
AoxI GGCC 1 cut(s) 357
ApeKI GCWGC 4 cut(s) 458, 461, 507, 581
ArsI GACNNNNNNTTYG 2 cut(s) 32, 64
AspLEI GCGC 1 cut(s) 507
AspS9I GGNCC 3 cut(s) 274, 358, 370
AsuC2I CCSGG 2 cut(s) 307, 341
AsuHPI GGTGA 2 cut(s) 190, 524
AvaI CYCGRG 1 cut(s) 89
AvaII GGWCC 2 cut(s) 274, 370
BanI GGYRCC 1 cut(s) 155
BauI CACGAG 2 cut(s) 684, 702
Bbv12I GWGCWC 1 cut(s) 96
BbvCI CCTCAGC 1 cut(s) 29
BbvI GCAGC 4 cut(s) 445, 448, 494, 568
BccI CCATC 1 cut(s) 182
BceAI ACGGC 3 cut(s) 88, 266, 280
BcgI CGANNNNNNTGC 2 cut(s) 683, 717
BciT130I CCWGG 1 cut(s) 374
BcnI CCSGG 2 cut(s) 307, 341
BcoDI GTCTC 2 cut(s) 40, 130
BfaI CTAG 2 cut(s) 348, 396
BfoI RGCGCY 1 cut(s) 508
BglI GCCNNNNNGGC 1 cut(s) 298
BisI GCNGC 6 cut(s) 132, 293, 459, 462, 508, 582
BlpI GCTNAGC 1 cut(s) 263
BlsI GCNGC 6 cut(s) 133, 294, 460, 463, 509, 583
Bme1390I CCNGG 3 cut(s) 307, 341, 374
Bme18I GGWCC 2 cut(s) 274, 370
BmeRI GACNNNNNGTC 1 cut(s) 325
BmeT110I CYCGRG 1 cut(s) 89
BmgT120I GGNCC 3 cut(s) 274, 358, 370
BmiI GGNNCC 3 cut(s) 62, 157, 371
BmrFI CCNGG 3 cut(s) 307, 341, 374
BmrI ACTGGG 1 cut(s) 355
BmsI GCATC 1 cut(s) 231
BmuI ACTGGG 1 cut(s) 355
BplI GAGNNNNNCTC 2 cut(s) 317, 349
Bpu10I CCTNAGC 1 cut(s) 29
Bpu1102I GCTNAGC 1 cut(s) 263
BpuMI CCSGG 2 cut(s) 307, 341
BsaAI YACGTR 1 cut(s) 563
BsaJI CCNNGG 1 cut(s) 296
Bsc4I CCNNNNNNNGG 3 cut(s) 366, 367, 672
Bse1I ACTGG 2 cut(s) 361, 531
BseBI CCWGG 1 cut(s) 374
BseDI CCNNGG 1 cut(s) 296
BseGI GGATG 1 cut(s) 222
BseLI CCNNNNNNNGG 3 cut(s) 366, 367, 672
BseMII CTCAG 4 cut(s) 24, 43, 277, 368
BseNI ACTGG 2 cut(s) 361, 531
BseRI GAGGAG 2 cut(s) 202, 218
BseXI GCAGC 4 cut(s) 445, 448, 494, 568
BshFI GGCC 1 cut(s) 359
BshNI GGYRCC 1 cut(s) 155
BsiHKAI GWGCWC 1 cut(s) 96
BsiHKCI CYCGRG 1 cut(s) 89
BsiSI CCGG 3 cut(s) 306, 341, 666
BslFI GGGAC 2 cut(s) 269, 537
BslI CCNNNNNNNGG 3 cut(s) 366, 367, 672
BsmAI GTCTC 2 cut(s) 40, 130
BsmBI CGTCTC 2 cut(s) 40, 130
BsmFI GGGAC 2 cut(s) 269, 537
BsnI GGCC 1 cut(s) 359
BsoBI CYCGRG 1 cut(s) 89
Bsp1286I GDGCHC 1 cut(s) 96
Bsp143I GATC 2 cut(s) 529, 710
Bsp1720I GCTNAGC 1 cut(s) 263
BspACI CCGC 4 cut(s) 129, 132, 293, 510
BspANI GGCC 1 cut(s) 359
BspCNI CTCAG 4 cut(s) 23, 42, 276, 367
BspLI GGNNCC 3 cut(s) 62, 157, 371
BspPI GGATC 1 cut(s) 537
BspT107I GGYRCC 1 cut(s) 155
BsrI ACTGG 2 cut(s) 361, 531
BssECI CCNNGG 1 cut(s) 296
BssMI GATC 2 cut(s) 529, 710
BssSI CACGAG 2 cut(s) 684, 702
Bst2BI CACGAG 2 cut(s) 684, 702
Bst2UI CCWGG 1 cut(s) 374
Bst4CI ACNGT 1 cut(s) 124
Bst6I CTCTTC 2 cut(s) 242, 380
BstBAI YACGTR 1 cut(s) 563
BstC8I GCNNGC 2 cut(s) 303, 696
BstDEI CTNAG 5 cut(s) 10, 29, 263, 354, 632
BstDSI CCRYGG 1 cut(s) 296
BstF5I GGATG 1 cut(s) 222
BstH2I RGCGCY 1 cut(s) 508
BstHHI GCGC 1 cut(s) 507
BstKTI GATC 2 cut(s) 532, 713
BstMAI GTCTC 2 cut(s) 40, 130
BstMBI GATC 2 cut(s) 529, 710
BstMWI GCNNNNNNNGC 2 cut(s) 298, 695
BstNI CCWGG 1 cut(s) 374
BstSCI CCNGG 3 cut(s) 305, 339, 372
BstV1I GCAGC 4 cut(s) 445, 448, 494, 568
BsuRI GGCC 1 cut(s) 359
BtgI CCRYGG 1 cut(s) 296
BtsCI GGATG 1 cut(s) 222
BtsI GCAGTG 1 cut(s) 180
BtsIMutI CAGTG 1 cut(s) 180
Cac8I GCNNGC 2 cut(s) 303, 696
CfoI GCGC 1 cut(s) 507
Cfr13I GGNCC 3 cut(s) 274, 358, 370
CseI GACGC 2 cut(s) 22, 685
Csp6I GTAC 1 cut(s) 535
CviAII CATG 1 cut(s) 57
CviQI GTAC 1 cut(s) 535
DdeI CTNAG 5 cut(s) 10, 29, 263, 354, 632
DpnI GATC 2 cut(s) 531, 712
DpnII GATC 2 cut(s) 529, 710
DraIII CACNNNGTG 1 cut(s) 635
DrdI GACNNNNNNGTC 1 cut(s) 281
DriI GACNNNNNGTC 1 cut(s) 325
DseDI GACNNNNNNGTC 1 cut(s) 281
Eam1104I CTCTTC 2 cut(s) 242, 380
Eam1105I GACNNNNNGTC 1 cut(s) 325
EarI CTCTTC 2 cut(s) 242, 380
EciI GGCGGA 1 cut(s) 118
Eco47I GGWCC 2 cut(s) 274, 370
Eco47III AGCGCT 1 cut(s) 506
Eco88I CYCGRG 1 cut(s) 89
EcoO109I RGGNCCY 1 cut(s) 274
EcoRII CCWGG 1 cut(s) 372
Esp3I CGTCTC 2 cut(s) 40, 130
FaeI CATG 1 cut(s) 60
FaiI YATR 8 cut(s) 58, 393, 405, 442, 490, 557, 598, 645
FaqI GGGAC 2 cut(s) 269, 537
FatI CATG 1 cut(s) 56
Fnu4HI GCNGC 6 cut(s) 132, 293, 459, 462, 508, 582
FokI GGATG 1 cut(s) 209
Fsp4HI GCNGC 6 cut(s) 132, 293, 459, 462, 508, 582
FspBI CTAG 2 cut(s) 348, 396
GlaI GCGC 1 cut(s) 506
GluI GCNGC 6 cut(s) 132, 293, 459, 462, 508, 582
HaeII RGCGCY 1 cut(s) 508
HaeIII GGCC 1 cut(s) 359
HapII CCGG 3 cut(s) 306, 341, 666
HgaI GACGC 2 cut(s) 22, 685
HhaI GCGC 1 cut(s) 507
Hin1II CATG 1 cut(s) 60
Hin6I GCGC 1 cut(s) 505
HinP1I GCGC 1 cut(s) 505
HindIII AAGCTT 1 cut(s) 696
HinfI GANTC 3 cut(s) 53, 326, 682
HpaII CCGG 3 cut(s) 306, 341, 666
HphI GGTGA 2 cut(s) 190, 524
Hpy188I TCNGA 2 cut(s) 40, 675
Hpy188III TCNNGA 1 cut(s) 592
Hpy99I CGWCG 1 cut(s) 155
HpyAV CCTTC 1 cut(s) 262
HpyCH4III ACNGT 1 cut(s) 124
HpyCH4IV ACGT 1 cut(s) 562
HpyCH4V TGCA 4 cut(s) 185, 222, 647, 689
HpyF10VI GCNNNNNNNGC 2 cut(s) 298, 695
HpyF3I CTNAG 5 cut(s) 10, 29, 263, 354, 632
HpySE526I ACGT 1 cut(s) 562
Hsp92II CATG 1 cut(s) 60
HspAI GCGC 1 cut(s) 505
Kzo9I GATC 2 cut(s) 529, 710
LmnI GCTCC 2 cut(s) 66, 399
Lsp1109I GCAGC 4 cut(s) 445, 448, 494, 568
LweI GCATC 1 cut(s) 231
MaeI CTAG 2 cut(s) 348, 396
MaeII ACGT 1 cut(s) 562
MaeIII GTNAC 1 cut(s) 637
MalI GATC 2 cut(s) 531, 712
MboI GATC 2 cut(s) 529, 710
MboII GAAGA 6 cut(s) 40, 187, 229, 397, 415, 418
MhlI GDGCHC 1 cut(s) 96
MluCI AATT 1 cut(s) 543
MlyI GAGTC 3 cut(s) 47, 335, 676
MmeI TCCRAC 1 cut(s) 63
MseI TTAA 1 cut(s) 501
MspI CCGG 3 cut(s) 306, 341, 666
MspR9I CCNGG 3 cut(s) 307, 341, 374
MvaI CCWGG 1 cut(s) 374
MwoI GCNNNNNNNGC 2 cut(s) 298, 695
NciI CCSGG 2 cut(s) 307, 341
NdeII GATC 2 cut(s) 529, 710
NlaIII CATG 1 cut(s) 60
NlaIV GGNNCC 3 cut(s) 62, 157, 371
NmeAIII GCCGAG 1 cut(s) 605
NmuCI GTSAC 1 cut(s) 637
PaeR7I CTCGAG 1 cut(s) 89
PfoI TCCNGGA 1 cut(s) 372
PkrI GCNGC 6 cut(s) 133, 294, 460, 463, 509, 583
PleI GAGTC 3 cut(s) 47, 334, 676
PpsI GAGTC 3 cut(s) 47, 334, 676
Ppu21I YACGTR 1 cut(s) 563
PpuMI RGGWCCY 1 cut(s) 274
Psp5II RGGWCCY 1 cut(s) 274
Psp6I CCWGG 1 cut(s) 372
PspGI CCWGG 1 cut(s) 372
PspN4I GGNNCC 3 cut(s) 62, 157, 371
PspPI GGNCC 3 cut(s) 274, 358, 370
PspPPI RGGWCCY 1 cut(s) 274
PspXI VCTCGAGB 1 cut(s) 89
RsaI GTAC 1 cut(s) 536
RsaNI GTAC 1 cut(s) 535
SaqAI TTAA 1 cut(s) 501
SatI GCNGC 6 cut(s) 132, 293, 459, 462, 508, 582
Sau3AI GATC 2 cut(s) 529, 710
Sau96I GGNCC 3 cut(s) 274, 358, 370
SchI GAGTC 3 cut(s) 47, 335, 676
ScrFI CCNGG 3 cut(s) 307, 341, 374
SduI GDGCHC 1 cut(s) 96
SfaNI GCATC 1 cut(s) 231
Sfr274I CTCGAG 1 cut(s) 89
SinI GGWCC 2 cut(s) 274, 370
SlaI CTCGAG 1 cut(s) 89
SmlI CTYRAG 1 cut(s) 89
SmoI CTYRAG 1 cut(s) 89
Sse9I AATT 1 cut(s) 543
SsiI CCGC 4 cut(s) 129, 132, 293, 510
SspMI CTAG 2 cut(s) 348, 396
StyD4I CCNGG 3 cut(s) 305, 339, 372
TaaI ACNGT 1 cut(s) 124
TaiI ACGT 1 cut(s) 565
TaqI TCGA 3 cut(s) 51, 90, 713
TasI AATT 1 cut(s) 543
TauI GCSGC 2 cut(s) 134, 295
Tru1I TTAA 1 cut(s) 501
Tru9I TTAA 1 cut(s) 501
TscAI CASTG 1 cut(s) 187
TseFI GTSAC 1 cut(s) 637
TseI GCWGC 4 cut(s) 458, 461, 507, 581
Tsp45I GTSAC 1 cut(s) 637
TspGWI ACGGA 3 cut(s) 53, 139, 205
TspRI CASTG 1 cut(s) 187
VpaK11BI GGWCC 2 cut(s) 274, 370
XhoI CTCGAG 1 cut(s) 89
XspI CTAG 2 cut(s) 348, 396
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.